Article Per Year (5 Year)
p-Index From 2021 - 2026
6.407
P-Index
This Author published in this journals
All Journal Agrovigor: Jurnal Agroekoteknologi Biotropika Jurnal Agroekoteknologi Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Journal of Tropical Biodiversity and Biotechnology BIOTROPIC The Journal of Tropical Biology Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis Agrisocionomics: Jurnal Sosial Ekonomi Pertanian Journal of Dedicators Community Jurnal Ilmiah Manajemen Publik dan Kebijakan Sosial JAIM (Jurnal Abdi Masyarakat) Bubungan Tinggi: Jurnal Pengabdian Masyarakat Indian Journal of Forensic Medicine & Toxicology Jurnal Agroteknologi J-ABDIPAMAS (Jurnal Pengabdian Kepada Masyarakat) Sewagati JATIMAS : Jurnal Pertanian dan Pengabdian Masyarakat BIO-CONS: Jurnal Biologi dan Konservasi Jurnal Ilmiah Pertanian Nasional Genbinesia Journal of Biology
Rasyadan Taufiq Probojati
Program Studi Agroteknologi, Fakultas Pertanian, Universitas Kediri, Kediri, Jawa Timur
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Physics Public Health Social Sciences Other

Published : 42 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search
Journal : Biotropika

 1 
Identification and Genetic Diversity of Pineapple Local Accessions from Kediri and Closely Related Species (Bromeliaceae) Based on matK Gene Rasyadan Taufiq Probojati; Hadiyanti, Nugraheni; Hapsari, Lia
Biotropika: Journal of Tropical Biology Vol. 12 No. 3 (2024)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2024.012.03.01

Abstract

Ananas comosus L. belongs to the Bromeliaceae family and has around eight subfamilies. Accessions of local pineapple have spread widely in several areas, one of which is in Kediri. However, some accessions and closely related species (Bromeliaceae) likely remain poorly understood relationships. The highly diverse morphological characters and low levels of sequence divergence in Bromeliaceae have been problematic in resolving the reconstruction of phylogenetic relationships. This study aimed to evaluate the efficiency of the matK gene as a DNA barcode to determine genetic diversity and reconstruct a phylogenetic tree in local pineapple and closely related species (Bromeliaceae). A total of 15 specimens were used in this research, of which 7 were local pineapple accessions from Kediri and eight were closely related species from the Bromeliaceae family. Whole genome DNA was isolated using a Tiangen kit and amplified using the polymerase chain reaction (PCR) technique using a specific primer. The results showed that matK was easily amplified with a DNA fragment length of 750-800 bp. The average composition value of each base was 36.9% T (U) bases, 30.1% in A bases, 15.9% in G bases, and 17.1% in C. BLAST analysis of the sequences showed that all samples were confirmed and identified according to the species names spread across local pineapple production areas (with query cover of 96-98%). However, the species names registered in the PBG database were confirmed to be different species but still in the same genus, i.e., samples B9-B14. The phylogenetic relationship analysis distinguished each accession into two large clades according to sub-families, i.e., Bromelioideae and Pitcairnioideae. This research shows that the matK gene is suitable and recommended as a DNA barcode for local pineapple and closely related species.
Co-Authors Abror, M. Daimul Achmad Masykur Ghazali Achmad Yogi Pambudi Adi Sofyan Ansori, Muhammad Agustia Dwi Pamujiati Ahadin Noor Ahmad Affan Ali Murtadlo Ahmad Haris Hasanuddin Slamet Aisyah Hadi Ramadani Alvida Mustika Rukmi Amaq Fadholly Andreas Zulkarnain Anggun Wulandari Anindita, Devina Cinantya Anshor, Yoyok Zakaria Arif Nur Muhammad Ansori Azkiyah, Lailatul Bambang Dwi Moeljanto Daimul Abror Daniel Happy Putra David Fajar Setyawan Dharmawan, Muhammad Alsyifaa Dhea Kharisma, Viol Dhony Hermanto Dian, Farida Aryani Didik Wahyudi Dings, Tim Godefridus Antonius Djoko Rahardjo Edy Kustiani Fahas, Exzanidan Fatikhatun Nikmatus Sholihah Firdausi, Muhammad Riza Firmansyah, Edo Prastiko Fredi Hadiyati, Nugraheni Hendri Cahyo Nugroho Hendy Hikam, Agus Mohammad Iin Nur A'idah Imam Mukhlash Junaidi Junaidi Junaidi Junaidi Kharisma, Viol Dhea Lia Hapsari Lia Hapsari Liya Agustina, Liya M. Daimul Abror Mariyono Mariyono Masyhuri, Aga Adi MOCH. FAIZUL HUDA Mochammad Saiful Efendi Mohammad Iqbal Mohammad Isa Irawan Mufiana Alfatin Mufiana Alfatin Muhammad Riza Firdausi muharram, Muhammad Murtadlo, Ahmad Affan Ali Nareswari, Aptika Hana Prastiwi Nata, Candra Dwi Nina Lisanty Nugraheni Hadiyanti Nurul Hidayat Nurul Ismillayli Nurul Jadid Mubarokati Ospa Pea Yuanita Meishanti Pamungkas, Kharisma Satria Prayoga, Reksa Nanda Putri, Rossanita Truelovin Hadi Rahadian Zainul Reksa Nanda Prayoga Rochmad Krissanjaya Rossanita Truelovin Hadi Putri Rukmini, Meme Ryan Edy Saputra Santoso, Yusuf Saptorini Saptorini, Saptorini Satriya Bayu Aji Satriya Bayu Aji, Satriya Bayu savitri, Lisa Setiawan, Andika Putra Sri Yunaning Subarkah, Minal Kholik Prima Supandji Supandji Supandji Supandji Supandji, Supandji Susi Ratnawati Tacharina, Martia Rani Tamam, Muhammad Badrut Tjatur Prijo Rahardjo Tjatur Prijo Rahardjo Turista, Dora Dayu Rahma Tutut Dwi Sutiknjo Ullah, Md. Emdad Viol Dhea Kharisma Wardatul Chamro’ Wicaksono, Adhityo Widi Artini Widyananda, Muhammad Hermawan Winata, Brama Sukma WIWIEK ANDAJANI Yulanda Antonius Yusuf, Taufan Achmad Gozali