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Warsidah, Warsidah
Program Studi Ilmu Kelautan, FMIPA Universitas Tanjungpura
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Journal : Pharmascience

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Uji Cemaran Mikroorganisme Pada Tiga Varian Daun Kratom (Mitragyna speciosa Korth.) Widian Sari, Margareta Lita; Masriani, Masriani; Warsidah, Warsidah; Yeni, Laili Fitri; Rasmawan, Rahmat
Jurnal Pharmascience Vol 11, No 1 (2024): Jurnal Pharmascience
Publisher : Program Studi Farmasi FMIPA Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v11i1.16230

Abstract

Tanaman kratom (Mitragyna speciosa Korth.) merupakan salah satu komoditas ekspor yang cukup menjanjikan dari Indonesia. Salah satu syarat suatu komoditas dapat diekspor harus memenuhi batasan kandungan mikrobiologi yang mengacu pada SNI dan AHPA. Bahan baku kratom yang terkontaminasi mikroorganisme, terutama mikroorganisme patogen akan membahayakan kesehatan konsumen. Oleh karena itu, penelitian ini bertujuan untuk menganalisis cemaran mikrobiologis tiga varian daun kratom, yaitu kratom merah, putih, dan hijau dalam bentuk serbuk kering simplisia. Uji cemaran mikrobiologi daun kratom meliputi cemaran bakteri Salmonella sp., Escherichia coli, dan kapang khamir. Uji cemaran bakteri Salmonella sp. dilakukan dengan menggunakan media Xylose Lysine Deoxycholate (XLD) dan Brilliat Green Agar (BGA) dengan suhu inkubasi 37oC selama 24 jam. Uji cemaran terhadap bakteri E. coli dilakukan dengan menggunakan media ColiformEscherichia coli Color Indicator dengan suhu inkubasi 35oC selama 24 jam. Penentuan kontaminasi kapang khamir menggunakan media Yeast and Mold Color Indicator dengan suhu 25oC selama 72 jam. Hasil uji yang telah dilakukan menunjukkan bahwa daun kratom merah, putih dan hijau tidak terkontaminasi bakteri Salmonella sp. dan E. Colitetapi terkontaminasi kapang khamir  yang lebih dari 104 koloni/gram. Kata Kunci: Ganja Borneo, Salmonella sp., Escherichia coli, Kapang Khamir, Koloni  The kratom plant (Mitragyna speciosa Korth.) is one of the most promising export commodities from Indonesia. One of the conditions for a commodity to be exported must meet the microbiological content limits referring to SNI and AHPA. Export commodities that do no meet the standards will be detained during the production and delivery process. Therefore, this study aims to analyze the microbiological contamination of three variants of kratom leaves, namely red, white and green kratom. Tests for microbiological contamination of kratom leaves include contamination of Salmonella sp., Escherichia coli, and yeast molds. Salmonella sp. bacteria contamination test carried out using Xylose Lysine Deoxycholate (XLD) and Brilliat Green Agar (BGA) media with an incubation temperature of 37oC for 24 hours. Contamination tests on E. coli bacteria were carried out using Coliform Escherichia coli Color Indicator media with an incubation temperature of 35oC for 24 hours. Determination of yeast contamination using Yeast and Mold Color Indicator media at 25oC for 72 hours. The results of the tests that have been carried out show that the red, white and green kratom leaves are not contaminated with Salmonella sp. and E. coli, but contaminated with yeast and Mold which is more than 104 colonies/gram.
Co-Authors . Apriansyah A. Kushadiwijayanto, Arie A.Nurrahman, Yusuf Abdul Hafiz, Iqbal Ade Hidayat, Ade Agustina, Sella Alfadzri, Andry Amelia, Debie Rizky Amran, Arman Andini, Dina Andryani, Semi Aninda, Dea Apriansyah Ari Widiyantoro Arief Nurrahman, Yusuf Aritonang, Anthoni B. Aritonang, Anthoni Batahan Ashari, Asri Mulya Astuti, Mega Sri Aswandi Aswandi, Aswandi Awlia, Isti Ayyash, Muhammad Yahya Bambang Kurniadi, Bambang BAMBANG SUJATMIKO Cahyany, Brigita Sari Kusuma Dahliana Dahliana dedi irawan Deni Deni Diah Wulandari Rousdy Dwi Gusmalawati Dzul Fadly Edwin Eny Enawaty Fadliah Nur, Andi Denisa Farhaby, Arthur M Fatmadiwi, Anggi Gusti Eva Tavita Haq, Izhar Amirul Hardan, Hardan Harianto Harianto Harianto Harianto, Miko Hasanah, Fadiah Amalia Helena, Shifa Hidayat, Maulana Idawati, Nora Ikha Ikha Safitri Irawan, Suhardi Irwan Irwan Nurdiansyah, Sy. Irwan Nurdiansyah, Syarif Izroil, Uwaiz Maulana Jayatri, Rahmi Jayuska, Afghani Juane, Mega Sari Junaidi, Levy Juniarti Juniarti Kalija, Tedi Ahmad Kasbutin, Hendri Kurniawanalamm Kushadiwijayanto , Arie Antasari Kushadiwijayanto, Arie Antasari Kusuma, Kwirinus Rio Kusumardana, Setra Laili Fitri Yeni Laksmitandari, Gania Ahlul Jannah Listy, Irma Lucky Hartanti, Lucky Lukas, Novan Luis Pernando Mardianto, Tomi Mardini, Delvia Devi Mariana Br. Surbakti Maryani Maryani Maser, Agnes Putri Masriani . Mega Sari Juane Sofiana Meidiantino, Stefans Melia Trianasta Minsas , Sukal Minsas, Sukal Muhardi Muhardi Muliadi Nabil, Ilhan Nail Neva Satyahadewi Nora Idiawati Noyanti, Resti Nur, Widia Nurdiansyah , Syarif Irwan Nurdiansyah, Irwan Syarif Nurdiansyah, Sy. Irwan Nurdiansyah, Syarif Irwan Nurhamidah, Anggia Nurrahman , Yusuf Arief Nurul Asikin Oktavia Oktavia Oktavia Paulus Paulus Pratiwi, Mutiara Anugerah Prayitno, Dwi Imam Priani , Wina Puji Ardiningsih Putri, Kristina Meissy Andini Raden Mohamad Herdian Bhakti Rafdinal Rafdinal Rahmat Rasmawan Ramadhan Sailendra, Pingkan Retno, Mayang Sari Rini Muharini Risko, Risko Rita Kurnia Apindiati Riza Linda Rody Putra Sartika Rudiyansyah Rudiyansyah Rudiyanto Rudiyanto, Rudiyanto S.J Sofiana, Mega Safitri , Ikha Sakina, Haiwatus Samira Samira Satyahadewi , Neva Selviana, Alya Siahaan, Jni Walbet Sihotang, Nesya Lia Simamora, Chinda Setia Lestari Sofiana, Mega Sj Sudiono, Gatot Sukal Minsas Suparnawati, Suparnawati Supriwanti, Supriwanti Surya Darma Sy. Irwan Nurdiansyah Syamswisna , Tahirah Hasan Tengku Riza Zarzani N Tia Nuraya Triono, Riski WAHYUNI Waldi Widian Sari, Margareta Lita yasnidar yasir Yuliono, Agus Yunivah, Adinda Yusuf Arief Nurrahman `B Aritonang, Anthoni