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Journal : Journal Of World Science

Exploration and Antimicrobial Activity Test of Secondary Metabolites from Endophytic Fungi of Curcuma Zedoaria from West Nusa Tenggara Mario, Mario; Rizky Prima, Sylvia; Primahana, Gian
Journal of World Science Vol. 4 No. 9 (2025): Journal of World Science
Publisher : Riviera Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58344/jws.v4i9.1505

Abstract

Antibiotic resistance has become a significant public health issue in Indonesia, prompting the search for alternative antimicrobial agents from natural sources. Endophytic fungi symbiotically living inside medicinal plants such as Curcuma zedoaria (temu putih) have the potential to produce bioactive secondary metabolites that could serve as new antibacterial agents. This study aimed to isolate endophytic fungi from the rhizome of temu putih originating from West Nusa Tenggara (NTB), evaluate the antibacterial activity of isolates grown on Potato Dextrose Broth (PDB) and Yeast Malt Broth (YMB), and identify as well as elucidate the structure of the secondary metabolite compounds produced. This experimental comparative study involved isolation of endophytic fungi by surface sterilization, culturing on PDB and YMB media, extraction with ethyl acetate, and antibacterial testing against Staphylococcus aureus ATCC 6538 and Escherichia coli ATCC 8739 using the microdilution method. Metabolite profiling was performed using HPLC-DAD, preparative HPLC for fractionation, LC-HRMS for molecular mass identification, and NMR spectroscopy for structural elucidation. Five endophytic fungal isolates were successfully obtained from the temu putih rhizome. Antibacterial activity was stronger in isolates cultured on PDB compared to YMB. From isolate TP 3 cultured in PDB, several secondary metabolite fractions were isolated, with fraction 4 (F4) containing linoleic acid as the pure compound. F4 demonstrated a Minimum Inhibitory Concentration (MIC) of 200 µg/mL against S. aureus, comparable to the TP 3 PDB isolate, while against E. coli, F4 showed MIC > 200 µg/mL and TP 3 PDB isolate showed an MIC of 100 µg/mL.
Co-Authors A.A. Ketut Agung Cahyawan W Abda Abda Ace Tatang Hidayat Ahmad Randy Aisya, Sitti Akhmad Darmawan Amalia, Senya Putri Anggraini, Meisi Anisa, Hida Arliani Nur Anjayani, Nuri Ariani, Novita Asep Saefurohman Asih Triastuti Atwita, Syelziva Yonda Barokah, Giri Rohmad Chang, Helen Cordova, Datu Muhammad Desi Harneti Putri Huspa Dewijanti, Indah D. Dewijanti, Indah Dwiatmi Dwi Retnowati Eti Nurwening Sholikhah Febi Nurilmala Fikriyah, Annisaa Nurul Filailla, Euis Harlita Harlita Hasidu, La Ode Abdul Fajar Hendris Hendarsyah Kurniawan, Hendris Hendarsyah Hening, Egiyanti Nur Widhia Hidayatullah, Sigit Hutagaol, Ricson Pemimpin Iffa Afiqa Khairani Imaniar, Lisana Husna Jekmal Malau Jepri Agung Priyanto, Jepri Agung Khalijah Awang Linar Zalinar Udin, Linar Zalinar LINDA SUKMARINI Mario Mario Maulidiyah Medi Hendra, Medi Megawati Megawati - Meti Indrowati Minarti Minarti Muhammad Nurdin N. Nurjanah Permatasari, Vera Prastya, Muhamad Eka Prastya, Muhammad Eka Prima, Sylvia Rizky Pulung, Maria Ludya Putra, Rayhan Helmyana Respati Tri Swasono Rhesi Kristiana Riana, Elisa Nurma Ridha, Rafifa Riskiana, Angger Arfi Rizna Triana Dewi Rosita, Lisa Rosmalena, Rosmalena Satyaningsih, Desy Shiono, Yoshihito Simbolon, Sumihartati Slamet Santosa Sofa Fajriah Sri Pujiyanto SUKIRNO Sukirno Sukirno Suryanti, Erma Tamhid, Hady Anshory TATI NURHAYATI Tjandrawati Mozef Tri Joko Raharjo UMI FATMAWATI Unang Supratman Vivitri Dewi Prasasty yati, indri Yogaswara, Radite Yuswan, Apriza Zahra, Aliya Azkia Zienitha, Anggie Meilinda