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All Journal Buletin Peternakan Biotropika Biology, Medicine, & Natural Product Chemistry Journal of Tropical Biodiversity and Biotechnology BIOMA : Jurnal Biologi Makassar Jurnal Biodjati Jurnal Penelitian Pendidikan IPA (JPPIPA) BIOEDUSCIENCE Jurnal Biologi Tropis Justek : Jurnal Sains Dan Teknologi Biosel: Biologi Science and Education Jurnal Pengabdian Magister Pendidikan IPA Biota BIO-EDU: Jurnal Pendidikan Biologi Jurnal Abdidas Koordinat : Jurnal Pembelajaran Matematika dan Sains Bioscientist : Jurnal Ilmiah Biologi Media Eksakta Journal Evaluation in Education (JEE) AGRI-TEK : Jurnal Penelitian Ilmu-ilmu Eksakta Interdisciplinary International Journal of Conservation and Culture Otus Education Jurnal Biologi dan Pendidikan Biologi Journal of Biology Science and Education
Manap Trianto
Universitas Tadulako
Agriculture, Biological Sciences & Forestry Arts Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Diversity and Composition of Insect Species Associated with Natural Rattan Habitats in Namo Village, Kulawi, Central Sulawesi Musdalifah Nurdin; Samsurizal M Suleman; Syech Zainal; Moh Sabran; Raya Agni; Manap Trianto
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.1289-1295

Abstract

Tropical forest ecosystems are home to diverse insect communities that play essential ecological roles, including pollination, decomposition, and nutrient cycling. Among the key forest plants, rattan (Arecaceae) provides both ecological and economic benefits and serves as an important microhabitat for various insect species. This study aimed to identify and analyze the diversity of insect species associated with natural rattan habitats in Namo Village, Kulawi District, Central Sulawesi, Indonesia. Insect sampling was carried out using sweep nets, pitfall traps, and yellow pan traps along rattan-dominated forest transects. A total of 125 individual insects were collected, representing 7 orders, 28 families, and 37 species. The order Hymenoptera showed the highest abundance (41.6%), dominated by Formicidae and Apidae families, followed by Coleoptera (25.6%) and Diptera (17.6%). The Shannon–Wiener Diversity Index (H’ = 2.84) indicated moderate species diversity with high evenness (E = 0.83). Functionally, the insect community consisted of pollinators (38.2%), herbivores (25.4%), decomposers (19.6%), and predators (16.8%). These findings demonstrate that natural rattan ecosystems support diverse and functionally balanced insect assemblages, reflecting stable ecological conditions and minimal anthropogenic disturbance. Conserving natural rattan stands is therefore crucial for maintaining biodiversity and sustaining ecological functions such as pollination and decomposition within tropical forest ecosystems.
Analysis of Nutritional Composition and Antioxidant Activity of Yogurt Enriched with Butterfly Pea (Clitoria ternatea L.) Extract Yulia Windarsih; I Made Budiarsa; Manap Trianto; Akram Akram; Abdul Ashari
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.831-841

Abstract

The growing demand for functional foods has encouraged the development of dairy products enriched with natural bioactive ingredients to improve nutritional value and health benefits. This study aimed to analyze the nutritional composition and antioxidant activity of yogurt enriched with butterfly pea (Clitoria ternatea L.) extract. Yogurt was prepared from fresh goat milk with varying concentrations of butterfly pea extract and evaluated for protein, fat, carbohydrate, moisture, ash, vitamin C contents, antioxidant activity, and sensory acceptance. Proximate analysis, UV–Vis spectrophotometry, and the DPPH method were used for chemical determinations, while a hedonic test assessed color, taste, aroma, and texture. Results showed that increasing butterfly pea extract significantly decreased protein and fat contents but increased vitamin C and antioxidant activity, with the highest values observed in yogurt containing 60% extract and 4% formulation (N60K2). Moisture and ash contents were not significantly affected. Sensory evaluation indicated that yogurt with 60% extract and 2% formulation (N60K1) was preferred regarding color, taste, aroma, and texture. Considering compliance with the Indonesian National Standard (SNI) and consumer acceptance, N60K1 was the optimal formulation. The findings suggest that butterfly pea extract can enhance the functional properties of yogurt, particularly its antioxidant potential, while maintaining acceptable sensory quality. This provides insights for developing functional dairy products with natural plant-based bioactives.
Molecular Identification of Lepidopteran Predators of Coccidae on Coffee Plants (Coffea canephora) in Sigi Regency I Made Budiarsa; Manap Trianto; Bahana Aditya Adnan
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.983-992

Abstract

This study aimed to identify the species of Lepidopteran predators that prey on soft scale insects (Coccidae) found on coffee plants in Sigi Regency using molecular techniques. DNA was extracted using the modified CTAB method (Doyle & Doyle, 1990). PCR amplification targeted the COI gene using universal primers LCO1490 and HCO2198 with a GeneAmp PCR System 9700. Visualization was done using 1% agarose gel and UV-transilluminator. Sequencing was conducted externally. Data were analyzed using BioEdit 7.2.5, BLAST, BOLD Systems, and MEGA11 software. The DNA sequence of the predator sample showed 97.91% similarity to Autoba rubra based on GenBank and 97.59% in BOLD Systems. Phylogenetic analysis confirmed a close genetic relationship with A. rubra, distinct from Eublemma. Morphological similarities with Eublemma were misleading, highlighting the accuracy of molecular identification. This study is the first to confirm the identity of a Lepidopteran predator of coffee scale insects in Indonesia using molecular techniques, revealing its close relation to Autoba rubra. The results correct previous morphological misidentifications and contribute new data on predator diversity in biological control contexts.
The Effectiveness of Honey Purity Testing from Several Regions in Central Sulawesi Province I Made Budiarsa; Manap Trianto; Akram Akram; Yulia Windarsih; Abdul Ashari
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.1231-1240

Abstract

Honey is a valuable natural product with nutritional, medicinal, and economic importance, but its high commercial value makes it prone to adulteration, which threatens consumer trust and product quality. This study aimed to evaluate the effectiveness of simple purity tests in identifying adulteration in honey samples collected from several regions in Central Sulawesi Province. A total of 12 honey samples from different bee species and floral sources were analyzed using seven tests, namely solubility, cloudiness and foam, heating, hexagonal pattern, seepage, raw fish, and onion tests. The results showed that most tests successfully distinguished pure honey from adulterated samples. Specifically, the solubility, cloudiness and foam tests, as well as heating, and raw fish tests indicated that 66.67% of the samples were pure, while the hexagonal pattern, seepage, and onion tests confirmed purity in all samples (100%). These findings demonstrate that simple, low-cost methods can provide reliable initial screening of honey purity and are suitable for routine use in local communities where advanced laboratory facilities are limited. The application of these tests is expected to support honey quality assurance, protect consumer trust, and promote the sustainable development of the beekeeping industry in Central Sulawesi.
Major Royal Jelly Protein 2 (mrjp2) Gene in Apis cerana from Central Sulawesi, Indonesia Budiarsa, I Made; Trianto, Manap; Akram, Akram; Windarsih, Yulia; Ashari, Abdul
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.39-44

Abstract

Indonesia is recognized as one of the world’s biodiversity hotspots, supporting a wide range of pollinating insects, including honey bee species of the genus Apis. Among these, Apis cerana is the most widely managed species and contributes significantly to national honey production, particularly in Sulawesi. However, the increasing economic value of honey has led to frequent cases of entomological origin fraud, in which honey is falsely labeled as being produced by a different bee species. This study aimed to determine the entomological origin of honey sold in Central Sulawesi using the Major Royal Jelly Protein 2 (mrjp2) gene as a molecular marker. Seven honey samples claimed as “forest honey” were analyzed through DNA extraction, PCR amplification using species-specific primers (CF-CR), and sequencing followed by BLAST-n analysis. All samples yielded high-quality DNA with purity ratios appropriate for PCR amplification. PCR successfully amplified the mrjp2 gene, producing fragments of approximately 201-208 bp, consistent with the expected size for A. cerana. Sequence analysis showed percent identity values ranging from 99.20% to 100% when compared with reference sequences of A. cerana. Based on molecular verification, three samples (H3, H5, and H7) did not match the seller’s claims and were confirmed to originate from A. cerana rather than A. dorsata binghami. These findings demonstrate that sequencing of the mrjp2 gene is an effective and reliable approach to authenticate the entomological origin of honey and to detect fraudulent labeling in commercial honey products.
Co-Authors Abd Rauf Abd. Rauf Abdul Ashari Abdul Hakim Laenggeng Abia, Hikma Achmad Ramadhan Achmad Ramadhan Adnan, Bahana Aditya Agung Dwi Kurniawan Ahmad Ramadhan Akram Akram Akram, Akram Alimudin, Sukmawati Amalia Buntu Amiruddin Kasim Anisa Anisa Aprilya, Dinda Ari Pratama Artha, Fandy Ashari, Abdul Asmara, Dudun Candra Asriani Hasanuddin Astija Bahana Aditya Adnan Baharuddin Hamzah Biung, Yelsan Kanan Burhanuddin, Alga Patricia Bustamin Bustamin Bustamin Bustamin Bustamin Carolus Noprianto Carolus Noprianto Daratul Maulydiia Delima, Nur Dirham Dirham Dirham Dirham Dirham Dirham Dirham Dirham Dirham Dirham Dirham, Dirham EFENDI Efendi Efendi Efendi Fajri Marisa Fajri Marisa Fajri Marisa Fajri Marisa Faried, Dirham Fariza, Ifat Fatma Dhafir Fatmah Dhafir Febriani, Vita Indri Febriawan, Aan Hamsa, Hamsa Hari Purwanto Hari Purwanto Hartina, Siti Hayyatun Mawaddah Herjayanti, Nur Herlangga Adiputra Hidyanisa, Dhea Hj. Musdalifah Nurdin I Ketut Suada I Made Budiarsa I Nengah Kundera Idham Cholik Ramadhan Isninar, Isnainar Ivan Permana Putra Kaini, Kaini Kaltisa, Kaltisa Khaerunnisa Kusumaningati, Lilis Tri Laksono Trisnantoro Lembah, Rocky Reviko T. Lestari M.P Alibasyah M. Abimanyu Marisa, Fajri Masriani . Masrianih Moh Dahri Kisman Moh Dahri Kisman Moh Dahri Kisman Moh Dahri Kisman Moh. Dahri Kisman Mohammad Jamhari Mursito S. Bialangi Musdalifah Nurdin Ni Gusti Ayu Galuh Candra Kirana Ni Putu Siswandari Ningsi, Ririn Apriani Noprianto, Carolus Novalina Serdiati Novi, Winda Cinthia NOVITASARI Nur Alam Nur Herjayanti Nur Herjayanti Nur Hikmah Nur'aini Nur'aini Nuraini Nuraini Nuraini Nuraini Nuraini Nuraini Nuraini Trianto Nurfadila A, Nurfadila A Nurfadilah Rahma Pratama, Rizaldy Pratama, Sandi Fransisco Puspitasari, Amira Putri, Regina Triana RAFIQA RAFIQA, RAFIQA Rahayu, Irma Septi Raya Agni Ritman Ishak Paudi Rizaldy Pratama Rocky Reviko T. Lembah Rocky RT Lembah Rosichon Ubaidillah Sabran , Moh. Saliyem, Saliyem Samsurizal M Suleman Samsurizal, M. Sulaeman Saridewi, Uut Sitti Nur Fadhilah Sitti Nuryanti Sri Hardianti sukmawati Sukmawati Sukmawati Sukmawati Sukmawati Sukmawati Suprianto Sutrisnawati Mardin Syech Zainal Syech Zainal Tiktiek Kurniawati Trianto, Nuraini Tuty Arisuryanti Varsha Salsabillah Wahyudi Wahyudi Warsih, Eko Winarsih Winarsih Windarsih, Yulia Yulia Windarsih Zasmita, Eka Zulaikhah Dwi Jayanti