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PENINGKATAN KUALITAS BIJI KAKAO (Theobroma cacao L) MELALUI FERMENTASI MENGGUNAKAN Lactobacillus sp. dan Pichia kudriavzevii Anja Meryandini; Asrianti Basri; Titi Candra Sunarti
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (848.014 KB) | DOI: 10.29122/jbbi.v6i1.3048

Abstract

The Improvement of Cacao Beans Quality through Fermentation by Using Lactobacillus sp. and Pichia kudriavzeviiABSTRACTIndonesia is one of the main cacao producers in the world. Indonesian cacao product is, however, relatively of low quality. Quality improvement of cacao beans is thus needed to increase added value of the product through such method as fermentation using bacteria and yeast. This study was conducted using four fermentation treatments, namely F1 (spontaneous fermentation without the addition of inoculum), F2 (addition of lactic acid bacteria inoculum), F3 (addition of yeast inoculum), F4 (addition of mixed lactic acid bacteria and yeast inoculum). The fermentation was carried out for 5 days. The parameters measured were the microbial cell number, pH, ethanol, total reducing sugar, and total acid concentration, as well as cacao seed quality. Results showed that, compared to the other treatments, the F4 treatment gave the best result, namely 83% of the cacao seeds being fermented, 2% non-fermented, 14% unfermented, 1% moldy, and 2% germinated. The liquid produced during the fermentation contained the highest reducing sugar of 123.38 mg·mL-1, the highest total acid of 24.42 mg·mL-1, and 3.57% ethanol.Keywords: cacao beans, fermentation, lactic acid bacteria, starter, yeast ABSTRAKIndonesia adalah salah satu penghasil kakao utama di dunia. Namun berdasarkan mutu, produk kakao Indonesia masih relatif tergolong rendah. Peningkatan kualitas biji kakao diperlukan untuk memberikan nilai tambah pada produk melalui metode seperti fermentasi menggunakan bakteri dan khamir. Penelitian ini dilakukan dengan empat perlakuan fermentasi yaitu F1 (fermentasi secara spontan tanpa penambahan inokulum), F2 (dengan penambahan inokulum bakteri asam laktat (BAL)), F3 (dengan penambahan inokulum khamir), F4 (dengan penambahan inokulum campuran bakteri asam laktat dan khamir). Fermentasi dilakukan selama 5 hari, dan parameter yang diukur selama fermentasi adalah jumlah mikroba, pH, kadar etanol, gula pereduksi, total asam serta kualitas biji. Hasil menunjukkan bahwa, dibandingkan perlakuan lainnya, perlakuan F4 memberikan hasil terbaik yaitu 83% biji terfermentasi, 2% tidak terfermentasi, 14% terfermentasi sebagian, 1% berjamur, dan 2% berkecambah. Cairan fermentasi tersebut mengandung gula reduksi yang paling tinggi 123,38 mg·mL-1, total asam tertinggi 24,42 mg·mL-1, dan kadar etanol mencapai 3,57%.Kata Kunci: bakteri asam laktat (BAL), biji kakao, fermentasi, khamir, starter
PENGOLAHAN LIMBAH CAIR PERIKANAN MENGGUNAKAN KONSORSIUMMIKROBA INDIGENOUS PROTEOLITIK DAN LIPOLITIK Devi Ambarwaty Oktavia; Djumali Mangunwidjaja; Singgih Wibowo; Titi Candra Sunarti; Mulyorini Rahayuningsih
AGROINTEK Vol 6, No 2 (2012)
Publisher : Agroindustrial Technology, University of Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrointek.v6i2.1975

Abstract

Fish processing industries produce liquid waste at different capacity in every level from production line. Because of less attention and knowledge, wastes become worse. The aim of this study was to discover potential microbial consortium that can degrade protein and fat of liquid waste from fish production processing. The consortiums formula were chosen from bacteria that  have the highest specific growth. Formulation A which degraded soluble protein than others. Formulation of B which degraded fat than others. All formulation had pH in range are 6 - 9. Liquid waste which outoclaved had degraded protein, soluble protein and pH parameters
Bioinsecticides Production by Bacillus thuringiensis subsp. aizawai using Agroindustrial by-Product in Solid Fermentation Kirana Sanggrami Sasmitaloka; Titi Candra Sunarti; Mulyorini Rahayuningsih
Jurnal Penelitian Pascapanen Pertanian Vol 13, No 1 (2016): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v13n1.2016.1-10

Abstract

Bacillus thuringiensis subsp. aizawai is one of well-known bioinsectiside resources, and utilized in organic farming for the replacement of chemical insecticide. The active compounds produced from fermentation process of B. thuringiensis subsp. aizawai are bacterial spores and protein crystal of δ-endotoxins, which are variously toxic to larvae of the Lepidoptera and Coleoptera. The use of bioinsecticides in Indonesia is still rarely because bioinsecticide marketed in Indonesia is still an import product so that price is relatively expensive. This problem can be overcome with producing bioinsecticide contain active B. thuringiensis using agroindustrial by product as a raw materials. This research compared the bioinsecticide production from several agroindustrial by product in solid fermentation of B. thuringiensis subsp. aizawai. This research aimed to find out the ability of B. thuringiensis subsp. aizawai using substrate combination of carbon source (cassava dregs, pulp of coffee, starch fractions of iles-iles, and sago dregs) and nitrogen source (tofu’s waste, peanut meal, palm kernel cake, and corn hominy) in bioinsecticides production using solid media cultivation. The research consisted of three stages, there were substrates characterization, microorganisms characterization, and bioinsecticides production. The main parameters to select the carbon and nitrogen sources are LC50 value and potential bioinsecticides products. The results showed that the best cultivation of B. thuringiensis subsp. aizawai contained in combination cassava dregs and palm kernel cake blends. This cultivation produced total cell counts 11.2 log CFU/g, viable spore counts 8.9 log CFU/g, LC50 0.04 μg/ ml and potential products 20000 IU/mg. Biopesticide produced can be used to kill Crocidolomia binotalis on cabbage. PRODUKSI BIOINSEKTISIDA OLEH Bacillus thuringiensis subs. aizawai PADA KULTIVASI MEDIA PADAT MENGGUNAKAN LIMBAH AGROINDUSTRI Bacillus thuringiensis subsp. aizawai merupakan salah satu bakteri yang digunakan untuk menghasilkan bioinsektisida dan digunakan pada pertanian organik untuk menggantikan pemakaian insektisida kimia. Komponen aktif yang diproduksi dari proses fermentasi B. thuringiensis subsp.aizawai adalah spora dan kristal protein δ-endotoksin, yang bersifat toksin terhadap larva Lepidoptera dan Coleoptera. Penggunaan bioinsektisida di Indonesia masih jarang karena biasanya berupa produk impor yang harganya mahal. Permasalahan ini dapat diselesaikan dengan memproduksi bioinsektisida menggunakan bahan aktif B. thuringiensis dan hasil samping agroindustri sebagai bahan baku. Penelitian ini bertujuan mengkaji kemampuan produksi B. thuringiensis subsp. aizawai pada beberapa jenis substrat sumber C (onggok, kulit kopi, fraksi pati iles-iles, dan ela sagu) dan sumber N (ampas tahu, bungkil kacang tanah, bungkil inti sawit, dan ampok jagung) dalam memproduksi bioinsektisida dari limbah agroindustri. Penelitian ini terdiri dari tiga tahap, yaitu karakterisasi substrat, karakterisasi isolat dan produksi bioinsektisida. Parameter utama untuk menetapkan sumber karbon dan nitrogen yang tepat adalah nilai LC50 dan potensi produk bioinsektisida. Hasil penelitian menunjukkan bahwa produksi bioinsektisida yang terbaik terdapat pada kultivasi B. thuringiensis subsp. aizawai menggunakan kombinasi onggok dan bungkil inti sawit. Pada kultivasi ini dihasilkan jumlah sel hidup 11.2 log CFU/g, jumlah spora 8.9 log CFU/g, nilai LC50 0.04 μg/ ml dan potensi bioinsektisida 20000 IU/mg. Bioinsektisida yang dihasilkan dapat digunakan untuk membasmi larva Croccidolomia binotalis pada tanaman kubis.
PRODUKSI ENZIM SELULASE OLEH AKTINOMISET MENGGUNAKAN FROND SAGU Sumarni Nompo; Anja Meryandini; Titi Candra Sunarti
Jurnal Penelitian Pascapanen Pertanian Vol 16, No 2 (2019): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v16n2.2019.80-89

Abstract

Fround Sagu adalah pucuk batang sagu yang masih dibungkus oleh pelepah dan tidak diamnfaatkan oleh industri pengolahan sagu. Fround sagu memiliki kandungan serat dengan kandungan selulosa yang tinggi serta berpotensi dijadikan bahan baku untuk produksi selulase. Enzim selulase diproduksi melalui kultivasi substrat cair frond sagu oleh Aktinomiset. Subtart berupa tepung sagu dan ampas frond sagu, diinokulasi oleh isolat HJ4 (3b) dan HJ5 (4b). Kedua isolat diremajakan dalam medium ISP-4 selama 5 hari, kemudian diinokulasikan ke dalam media tepung frond sagu dan ampas frond dan diinkubasi dalam shaker pada suhu runag selama 9 hari. Kedua isolat Aktinomiset mampu menghasilkan enzim selulase pada kedua substrat dan metode kultivasi. Isolat HJ4 (3b) dan HJ4 (5b) pada perlakuan kultivasi substrat padat ampas frond sagu menghasilkan aktivitas spesifik yaitu endoglukase (CMCase) tertinggi yaitu 0.314 U mg-1 dan 0.294 U mg-1 dan aktivitas spesifik enzim eksoglukanase (FPase) yaitu 0.269 U mg-1 dan 0.258 U mg-1, sedangkan pada perlakuan kultivasi substat padat menggunakan tepung frond sagu dihasilkan aktivitas spesifik endoglukanase masing-masing sebesar 0.258 U mg-1 dan 0.254 U mg-1 serta aktivitas spesifik eksoglukanase 0.205 U mg-1 dan 0.198 U mg-1. Production of Cellulase Enzyme by Actinomycet Using Sago FrondSago frond is the upper part of sago trunk which is still wrapped by leaflet, and is not used by the sago processing industry. Sago frond contains fiber with high cellulose content that could potentially be used by as raw material for cellulase production. Cellulase enzymes were produced through both solid-state and submerged cultivation of sago frond by Actinomicycetes. Two substrates, sago frond flour and pulp of sago fronds, were inoculated by isolate HJ4 (3b) and HJ4 (5b). Both isolates were rejuvenated in Sp-4 medium for 5 days, then were inoculated into substrate of frond flour and hampas, and were incubated in a shaker at room temperature for 9 days. Both Actinomycetes isolates were able to produce cellulase enzymes by using both substrates and cultivation methods. The isolates of HJ4 (3b) and HJ4 (5b) by using pulp and solid-state cultivation produced the highest endoglucanase (CMCase) specific activity of 0.294 U mg-1 and 0.276 U mg-1 and exoglucanase (FPase) substrate specific activity os 0.252 U mg-1 and 0.241 U mg-1, while in the solid-state cultivation and by using sago fronds flour resulted in specific endoglucanase activities which were 0.242 U mg-1 and 0.238 U mg-1 and exoglucanase specific activities 0.192 U mg-1 and 0.185 U mg-1, respectively.
SINTESIS NANOSELULOSA DARI SERAT NANAS DAN APLIKASINYA SEBAGAINANOFILLERPADA FILM BERBASIS POLIVINIL ALKOHOL Evi Savitri Iriani; Kendri Wahyuningsih; Titi Candra Sunarti; Asep Wawan Permana
Jurnal Penelitian Pascapanen Pertanian Vol 12, No 1 (2015): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v12n1.2015.11-19

Abstract

A variety of attempts have been made to reduce the dependence on petroleum raw materials based plastics which are limited supply the mechanical properties close to conventional plastic. The objectives of research was to know the effect of  pineapple nanocellulose effective in increasing tensile strength and elongation, but the higher addition (of up to 50%) resulted in decreased elongation. The addition and without glycerol
PEMANFAATAN BIOMASSA LIGNOSELULOSA AMPAS TEBU UNTUK PRODUKSI BIOETANOL Euis Hermiati; Djumali Mangunwidjaja; Titi Candra Sunarti; Ono Suparno; Bambang Prasetya
Jurnal Penelitian dan Pengembangan Pertanian Vol 29, No 4 (2010): Desember, 2010
Publisher : Badan Penelitian dan Pengembangan Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jp3.v29n4.2010.p121-130

Abstract

Utilization of lignocellulosic biomass from sugarcane bagasse for bioethanol production Sugarcane bagasse is one of potential lignocellulosic biomass for energy through physical, chemical or biological conversion. The material is renewable and abundantly available, especially as wastes or by-products of sugarcane industries. Of many conversion processes, lignocellulosic conversion to ethanol becomes focus of interest recently, since ethanol can be further used as biofuel to substitute gasoline for transportation. Lignocellulosic material, including sugarcane bagasse mainly consists of three components, namely cellulose, hemicellulose, and lignin. The conversion of these materials basically consists of pretreatment, cellulose hydrolysis, sugar fermentation to ethanol, and purification of ethanol. Production cost of this conversion is still high; therefore, many researches have been conducted to improve the conversion process, either pretreatment, hydrolysis, fermentation or purification, so that the cost could be reduced. This paper reviewed literatures on potential and characteristics of lignocellulosic materials, especially sugarcane bagasse, and conversion of these materials to ethanol. There is as much as 614,827 kL/year of ethanol potentially produced from sugarcane bagasse resulted from sugarcane factories in Indonesia. This amount of ethanol would have a great contribution to fulfill 1.10 million tons demand of ethanol. However, there are still some recalcitrans in production and implementation of lignocellulosic bioethanol, especially due to the unproven conversion technology of lignocellulosic biomass to ethanol and the high production cost. Therefore, government policies in supporting research and development, providing special incentives for sugarcane factories that produced ethanol from sugarcane bagasse, and giving subsidy to reduce bioethanol price, are needed.Keywords: Sugar by products, lignocellulose, conversion, ethanol
PRODUKSI DAN APLIKASI PATI NANOPARTIKEL Christina Winarti; Titi Candra Sunarti; Nur Richana
Buletin Teknologi Pasca Panen Vol 7, No 2 (2011): Buletin Teknologi Pascapanen Pertanian
Publisher : Buletin Teknologi Pasca Panen

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pati merupakan biopolimer alami yang banyak digunakan sebagai bahan baku industri. Teknologi nanopartikel akan memperbaiki karakteristik pati sehingga memiliki viskositas suspensi rendah pada konsentrasi yang relatif tinggi, dan mempunyai kekuatan pengikatan yang tinggi karena luas permukaan aktif yang besar. Pati nanopartikel bisa diaplikasikan sebagai penguat bahan pengemas, komposit biodegradable, matriks pembawa bahan aktif pangan atau obat, coating (pelapis) dan perekat biodegradable. Produksi pati nanopartikel bisa dilakukan karena struktur pati memungkinkan membentuk nanopartikel secara spontan (self assembly) dengan cara hidrolisis asam atau enzim menghasilkan pati nanokristal, sedangkan presipitasi pembentukan kompleks dari pati tergelatinisasi atau emulsifikasi menghasilkan pati nanopartikel. Produksi nanokristal dengan hidrolisis asam kuat pada suhu di bawah suhu gelatinisasi akan menghasilkan kristalit berukuran nano yang digunakan sebagai penguat atau pengisi polimer lain seperti karet, plastik atau pati termoplastis. Nanopartikel yang dihasilkan dari pati tergelatinisasi menghasilkan pati berukuran nano yang lebih amorf dan berfungsi sebagai matriks pembawa bahan aktif. Dalam tulisan ini dipaparkan mengenai preparasi/ produksi, aplikasi serta peluang dan tantangan yang dihadapi dalam pengembangan pati nanopartikel.
BLOCKCHAIN TECHNOLOGY IN AGRO-INDUSTRIAL SUPPLY CHAIN: REVIEW Ratna Ekawati; Yandra Arkeman; Suprihatin Suprihatin; Titi Candra Sunarti
Journal of Engineering and Management in Industrial System Vol 9, No 2 (2021)
Publisher : Badan Penerbit Jurnal, Faculty of Engineering, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jemis/2021.009.02.2

Abstract

This paper provides a review of the application of blockchain technology in industrial supply chains in general. It aims to investigate how current blockchain technology is and its applications and to look for research loopholes that have not been done before. The theory of several research papers that have been published in journals to date, along with several reports from the grayish literature as a means of streamlining judgments about growing blockchain-based supply chains is included in this review. The research step is to identify papers based on the year of publication so that a theory-based understanding of blockchain technology in the Agro-Industrial Supply Chain ecosystem, between 2007 and 2019. Blockchain technology in the agro-industry supply chain described in the framework in 2018 is 6.57%, while the implication for the case study is 9.11% and identified in the hypothesis with a questionnaire regarding blockchain recognition and exploration so that 4.5% of the blockchain model simulation. Based on the graph, there are 52% of blockchain research articles on the agro-industrial supply chain will be followed up by researchers, thereby increasing the value chain in agro-industry more. Research that discusses the value (value) in the supply chain that occurs from upstream to downstream in the agro-industrial supply chain ecosystem is very little, only around 2%. Excellence blockchain can identify the stakeholders that exist in the supply chain in each ecosystem from upstream to downstream processes so that it can transform a rigid and uncertain supply chain system into an efficient performance supply chain with transparency and trust between stakeholders so that integration, collaboration, and accuracy accurate data information
INNOVATION STRATEGY TO IMPROVE THE COMPETITIVENESS OF MICRO, SMALL, AND MEDIUM ENTERPRISES OF BANDAR LAMPUNG BANANA CHIPS Hartami Dewi; Mohamad Syamsul Maarif; Titi Candra Sunarti
Indonesian Journal of Business and Entrepreneurship (IJBE) Vol. 3 No. 1 (2017): IJBE, Vol. 3 No. 1, January 2017
Publisher : School of Business, IPB University (SB-IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17358/ijbe.3.1.64

Abstract

Innovation is needed by micro, small, and medium enterprises to grow and develop into large businesses. However, that innovations are limited by existing constraints such as limited number of employees, total assets, total revenues and total budget for innovation. Human resources or the entrepreneur is known to be an important factor in improving the performance and innovation of SMEs or UMKM. This research aims to formulate strategies given the constraints of innovation, human resource capacity and opportunities for innovation in improving competitiveness. The analytical method used is confirmed method, the evaluation model of structural equation modeling (SEM) and the analytic hierarchy process (AHP). The analysis showed an effect of human resources amounting to 0.761 on the settlement of the constraints and influence of 0.806 to successful innovation. The main obstacle that have to be resolved are human resources, promotion, managerial finance and administration, location, and marketing. The innovation that most influence in purchasing decisions and according to the ability of SMEs are service innovation, organization, business model, supply chain, and marketing. The best strategy formulation that can be done in improving the competitiveness of businesses are participation in exhibitions and entrepreneurship seminars, technical assistance, training and the online marketing promotion, as well as the provision of machinery and appropriate process equipment.Keywords: innovation, competitiveness, structural equation modeling, analytic hierarchy process
Characterization of Chemical and Physical Properties of Hydroxypropylated and Cross-linked Arrowroot (Marantha arundinacea) Starch Rijanti Rahaju Maulani; Dedi Fardiaz; Feri Kusnandar; Titi Candra Sunarti
Journal of Engineering and Technological Sciences Vol. 45 No. 3 (2013)
Publisher : Institute for Research and Community Services, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.eng.technol.sci.2013.45.3.1

Abstract

The modern food industry and a variety of food products require tolerant starch as raw material for processing in a broad range of techniques, from preparation to storage and distribution. Dual modification of arrowroot starch using hydroxypropylation and cross-linking was carried out to overcome the lack of native arrowroot starch in food processing application. The modifications applied were: combined propylene oxide (8%, 10%, and 12%); sodium tri meta phosphate/STMP (1%, 2%, and 3%); and sodium tri poly phosphate/STPP (4%, 5%, and 6%). These modifications significantly affected the composition of the amylose and amylopectin and the amount of phosphorus in the granules. Higher amounts of phosphate salt gave a higher phosphorus content, which increased the degree of substitution (DS) and the degree of cross-link. Arrowroot starch that was modified using a concentration of 8-10% propylene oxide and 1-2% STMP : 3-5% STPP produced a starch with < 0.4% phosphorus content. A higher concentration of propylene oxide provided a higher degree of hydroxypropyl. The changed physical properties of the modified granular arrowroot starch were examined through SEM testing, and its changed crystalline patterns through X-ray diffraction measurements. Especially, provision of a high concentration of propylene oxide (12%) combined with 3% STMP : 6% STPP affected the granular morphology and the crystallinity.
Co-Authors Adisalamun Adisalamun Agus Setiyono AHMAD JUNAEDI Aisyah, Nadira Akhmad Endang Zainal Hasan Anas Miftah Fauzi Andi Nurul Aulia Sari Andika Susantri Ani Suryani Ani Suryani Anja Meryandini Anja Meryandini ANJA MERYANDINI Anja Meryandini Anja Meryandini Anja Meryandini Anja Meryandini Anja Meryandini ANJA MERYANDINI Anja Meryandini Anja Meryandini Anuraga Jayanegara APRILIA NAOMI Aprilia Nurhasna Aris Purwanto Asep Wawan Permana Asep Wawan Permana, Asep Wawan Asrianti Basri Asrianti Basri Ayu Rahayu SARASWATI Bambang Prasetya Basri, Asrianti Basri, Asrianti Besty Maranatha Birahy, Deford Cristy Bruce A. Welt, Bruce A. Cahyana, Purwa Tri Christina Winarti Christina Winarti Dede Heri Yuli Yanto Dedi Fardiaz Devi Ambarwaty Oktavia Dewi Diniaty Djumali Mangunwidjaja Djumali Mangunwidjaja Djumali Mangunwidjaja Djumali Mangunwidjaja Djumali Mangunwidjaja Dwi Setyaningsih Eka Putri EKA RURIANI EKA RURIANI Eka Ruriani Elisa Julianti Elly Rosyidah Endang Warsiki Endang Warsiki Erliza Noor Euis Hermiati Evi Savitri Iriani Evi Savitri Iriani, Evi Savitri Faqih Udin FARAH FAHMA Februadi Bastian Feri Kusnandar Ferry Mutia FERY MUTIA Fiora Helmi Frangky J. Paat Glisina Dwinoor Rembulan Hadi Karia Purwadaria, Hadi Karia Hari Eko Irianto Hartami Dewi Hartrisari Hardjomidjojo Hasrul Satria Hasrul Satria, Hasrul Ifah Munifah Ika Amalia Kartika Ilham Marvie Indah Yuliasih IRA ERDIANDINI, IRA Irmayanti Irmayanti Irmayanti Irmayanti, Irmayanti Irvan Anwar Iskandar Lubis Kendri Wahyuningsih Kendri Wahyuningsih, Kendri Khaswar Syamsu Kirana Sanggrami Sasmitaloka Kurniawan Yuniarto Kurniawan, Dede Yudo Laily, Noer Lala Nurmala Lisman Suryanegara M. EDY SOFIYANTO Machfud Machfud Mala Nurilmala Manalu, Lamhot Maranatha, Besty Marimin , Matasik, Delfania Maya Melati Mohamad Syamsul Ma’arif Mulyorini Rahayuningsih Nastiti Siswi Indrasti Nida El Husna Niken Financia Gusmawati NISA RACHMANIA MUBARIK Nisfatin Shofiana Nur Richana Nuri Andarwulan Odi Andanu Ono Suparno Paramuji, Muji Pasaribu, Fatimah J Petrus Adam Pramana, Yanuar Sigit Purwanto, Yanuar Jarwadi Purwoko Purwoko Pustika Adwiyani RAMADHAN, ZULFA AULIA Ratna Ekawati Ratna Ekawati Ridwansyah Ridwansyah Rijanti Rahaju Maulani Rijanti Rahaju Maulani Rijanti Rahaju Maulani Rini Purnawati Rismawati . RM Muhammad Nur Fauzan Roh santoso Ronny Soputan Rosyidah, Elly Ruka Yulia SAFITRI NURLAELA Salfauqi Nurman, Salfauqi Sanbein, Primus Sapta Raharja Savira Astri Adriana Setyo Purwanto Singgih Wibowo Sitti Rahbiah Akram Stanislaus Aditya Agung Sugiyanta Sukard Sumarni Nompo Suprihatin Suprihatin Suprihatin Suprihatin Suprihatin Suprihatin Suprihatin Suyono, Meisy Nawang Taufik Hidayat Tjahja Muhandri Tryanisa Ridla Amalia Utomo, Prasetyo Hadi Vestika Iskawati Wahidul Hijah Wahyu Widosari Wawan Hermawan Widiatmaka Widiatmaka Widosari, Wahyu WULAN, RAHAYU Yandra Arkeman Yaya Rukayadi Yuana Susmiati Yulia, Ruka YULIANA, META YULIN LESTARI Yulistika, Efri Yunus Triyonggo, Yunus Yusmiati Yusmiati Zahrani, Siti Mutia