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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan Jurnal Teknologi Industri Pertanian E-jurnal Agro-Industri Indonesia Reaktor Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Agrointek Jurnal Penelitian Pascapanen Pertanian Jurnal Penelitian dan Pengembangan Pertanian Buletin Teknologi Pasca Panen Journal of Engineering and Management in Industrial System Indonesian Journal of Business and Entrepreneurship (IJBE) Journal of Engineering and Technological Sciences agriTECH Jurnal Sumberdaya Hayati Science and Technology Indonesia Jurnal Bioteknologi & Biosains Indonesia (JBBI) Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology EDUFORTECH Journal of Tropical Crop Science Menara Perkebunan Journal of Applied Engineering and Technological Science (JAETS) Indonesian Journal of Electrical Engineering and Computer Science Jurnal Penelitian dan Pengembangan Pertanian International Journal of Mechanical Engineering Technologies and Applications (MECHTA) Jurnal Agroekoteknologi Terapan ABDI PUSTAKA: Jurnal Perpustakaan dan Kearsipan Proceedings of International Conference on Multidiciplinary Research Journal of Applied Agricultural Science and Technology Prosiding Seminar Nasional Universitas Serambi Mekkah PERTANIAN TROPIK Pharmaceutical Sciences and Research (PSR) Makara Journal of Science Makara Journal of Technology Jurnal Natural Journal of Sciencce Technology and Visual Culture JURNAL REKAYASA KIMIA & LINGKUNGAN

Titi Candra Sunarti

Departemen Teknologi Industri Pertanian Fakultas Teknologi Pertanian Institut Pertanian Bogor Jl. Lingkar Akademik, Kampus IPB Darmaga, Bogor 16680; Telp. (0251) 8621974

Author-ID : 2059856

Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Neuroscience Physics Social Sciences Veterinary Other

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Rekayasa Proses Hidrolisis Pati dan Serat Ubi Kayu (Manihot utilissima) untuk Produksi Bioetanol Yuana Susmiati; Dwi Setyaningsih; Titi Candra Sunarti
agriTECH Vol 31, No 4 (2011)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Ethanol production from cassava (Manihot utilissima) usually uses enzymatic process for starch hydrolysis. Enzymatichydrolysis by α-amylase and amyloglucosidase enzymes are not able to convert cassava fibers into sugars. Dilute acid hydrolysis is applied to convert both starch and fibers, which will increase the yield of simple sugars as fermentable sugars and resulting in high ethanol production. In this research there are two steps of dilute acid hydrolysis, first for starch hydrolysis at H SO concentration of 0.1-0.5 M, 5-15 minutes and second for fiber hydrolysis at 0.5-1.0 24M H SO , 10-20 minutes, at the same temperature of 121-127 oC and pressure of 1.0-1.5 atm. The disadvantage of acid hydrolysis is the formation of toxic compounds such as hydroxymethyl furfural (HMF) which is inhibited yeast fermentation. Therefore, acid hydrolyzates were detoxified with NH OH before use as fermentation substrate. The best starch hydrolysis condition was obtained at 0.4 M H SO for 10 minutes which gave 257.37 g/l of total sugars, 24229.38 g/l of reducing sugars, 89.59 of dextrose equivalent (DE) and 0.57 g/l of HMF. While the best fiber hydrolysis performed at 1.0 M H SO solution for 20 minutes which gave 79.74 g/l of total sugars, 70.88 g/l of reducing sugars, 2488.99 of DE and 0.0142 g/l of HMF. Single direct acid hydrolysis was the most suitable substrate for yeast fermentationwith the ethanol concentration of 5.7 % (w/v) and 30.5 % (w/w) of ethanol yield. This result is comparable with enzymatic hydrolysis which gave ethanol yield of 30 % (w/w).ABSTRAKProduksi etanol dari ubi kayu biasanya menggunakan enzim untuk menghidrolisis pati. Hidrolisis secara enzimatismenggunakan enzim α-amilase dan amiloglukosidase tidak mampu mengkonversi serat menjadi gula. Hidrolisis asam berkonsentrasi rendah dilakukan untuk mengkonversi pati dan serat, sehingga gula-gula sederhana yang dapat difermentasi meningkat dan menghasilkan produksi etanol tinggi. Pada penelitian ini ada dua tahap hidrolisis menggunakan asam berkonsentrasi rendah, yaitu tahap pertama untuk menghidrolisis pati dengan konsentrasi H SO 0,1-0,5 M selama 5-15 menit dan tahap kedua untuk menghidrolisis serat dengan kensentrasi H SO 0,5-1,0 M selama 2410-20 menit pada suhu dan tekanan sama, yaitu 121-127 oC dan 1,0-1,5 atm. Kekurangan pada hidrolisis asam adalahterbentuknya senyawa toksik seperti hidroksimetil furfural (HMF) yang mengganggu fermentasi khamir. Oleh karena itu hidrolisat asam didetoksifikasi menggunakan NH OH sebelum digunakan sebagai substrat fermentasi. Kondisi terbaik hidrolisis pati diperoleh pada konsentrasi H SO 0,4 M selama 10 menit dengan nilai total gula 257,37 g/l, 24gula pereduksi 229,38 g/l, dextrose equivalent (DE) 89,59 dan HMF 0,57 g/l. Selain itu kondisi terbaik hidrolisis serat diperoleh pada konsentrasi H SO 1,0 M selama 20 menit dengan nilai total gula 79,74 g/l, gula pereduksi 70,88 g/l, 24DE 88,99 dan HMF 0,0142 g/l. Hidrolisat asam yang paling sesuai digunakan sebagai substrat fermentasi adalah darihidrolisis satu tahap tanpa pemisahan serat yang menghasilkan etanol dengan konsentrasi 5,7 % (b/v) dan rendemen etanol 30,5 (b/b). Hasil tersebut tidak berbeda jauh dengan hidrolisis enzimatis yang mengasilkan rendemen etanol 30% (b/b).

PENINGKATAN KECERAHAN PADA PROSES SINTESIS SURFAKTAN NONIONIK ALKIL POLIGLIKOSIDA (APG) BERBASIS TAPIOKA DAN DODEKANOL Februadi Bastian; Ani Suryani; Titi Candra Sunarti
Reaktor Volume 14, Nomor 2, Oktober 2012
Publisher : Dept. of Chemical Engineering, Diponegoro University

Alkylpolyglycosides (APG) is a nonionic surfactant that has been getting some green labels such asEcocert, EU Eco-flower and Green Seal as an environmentally friendly surfactant. Sugar is the mainraw material which is supplied the hydrophilic group, and fatty alcohol as hydrophobic group. Someundesirable compounds formed during the APG production and caused low quality. The aim of thisresearch to increase the quality and performance of APG, by controlling its process. Addition 0-10%of activated carbon and 0-0.3% of NaBH4 in APG pre-purification process; 2% (w/w) of H2O2, 35%and 500 ppm of MgO in the bleaching process were examined to process high quality and highperformance of APG. The best APG was obtained from purification step by addition 0% of activatedcarbon and 0.2% of NaBH4, with the characteristics of clarity of 59.02(%T); the ability to reducesurface and interfacial tensions at 1% concentration were 61.94% and 95.6% respectively; 81.71%of stability of emulsion, 62.5% of foam height and stable up to 315 minutes. Alkil Poliglikosida (APG) merupakan surfaktan nonionic yang telah mendapatkan beberapa greenlabel seperti Ecocert, EU Eco-flower Green Seal dan sebagainya sebagai surfaktan yang ramahlingkungan. Bahan dasar APG yaitu gula untuk membentuk gugus hidrofiliknya dan fatty alcoholuntuk membentuk gugus hidrofobiknya. Permasalahan dalam produksi APG adalah timbulnya warnagelap yang tidak dikehendaki yang menyebabkan penurunan mutu APG. Tujuan dari penelitian iniyaitu untuk meningkatkan mutu dan kinerja dari APG. Untuk meningkatkan kecerahan APG, makasebelum dilakukan proses destilasi dilakukan penambahan arang aktif 0-10% serta NaBH4 0-0,3%.Pada proses pemucatan ditambahkan 2% (b/b) H2O2 35% dan MgO 500 ppm. Hasil terbaik yaitupenambahan arang aktif 0% dan NaBH4 0,2% dengan karakteristik kecerahan 59,02(%T);kemampuan menurunkan tegangan permukaan dan antar muka pada konsentrasi 1% sebesar 61,94%dan 95,6%; kestabilan emulsi 81,71%, tinggi busa 62,5% dan umur busa 315 menit.

Seleksi Bakteri Asam Laktat dan Pemanfaatannya Sebagai Starter Kering Menggunakan Matriks Tapioka Asam Ira Erdiandini; Titi Candra Sunarti; Anja Meryandini
Jurnal Sumberdaya Hayati Vol. 1 No. 1 (2015)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.1.1.26-33

The development of industrial fermentation food could not separate with the availability of culture starter that suffice to support its production. Dried starter can be an option to use in fermentation industry because it can be stored for longer time without rejuvenation. However, in the process of production of dried starter needs the matrix to maintain cell viability, economically and availability of raw material. This research was conducted to use selected dried starter of indigenous lactic acid bacteria by using sour cassava starch matrix. Eleven local isolates lactic acid bacteria isolates from spontaneous fermentation of carbohydrates commodity were selected based on their acid production capabilities and antibiotics susceptibilities. Isolate of E 1222 showed the best result and was identified as Pediococcus pentosaceus. The isolate was encapsulated with sour cassava starch matrix for making dried starter by using freeze dryer and spray dryer. Freeze dried starter culture could maintained the cell viability higher than spray dried starter culture i.e 10.34 log CFU/g and 8.91 log CFU/g, respectively. Finally, freeze dried starter culture could maintain the percentage of cell viability until 89.38% during four-weeks storage at 4 oC.

Formula Media Pertumbuhan Bakteri Asam Laktat Pediococcus pentosaceus Menggunakan Substrat Whey Tahu SAFITRI NURLAELA; TITI CANDRA SUNARTI; ANJA MERYANDINI
Jurnal Sumberdaya Hayati Vol. 2 No. 2 (2016)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.2.2.31-38

The lactic acid bacteria (LAB) and its lactic acid are commonly used to preserve the food and to extend the food’s shelf life. MRS media is a growth medium for LAB, but it is not feasible for industrial scale application. Cheaper substrate from agriculture products is therefore required, such as tofu whey, which is potential to be used as LAB medium. Tofu whey contains important components to support the LAB growth, but it needs C source (5% of glucose) and nitrogen source (1% ammonium sulphate or urea) supplementations. This study aimed to investigate the influence of N-source to Pediococcus pentosaceus growth and its capability in producing acid compounds. The result showed that addition of urea increased pH fermentation, contrarily to that ammonium sulphate supplementation. The highest bacterial growth rate (μmaks) was observed on media with urea (0.43 jam-1), while the highest acid production was occured on media with ammonium sulphate (9.13 mg/mL). Supplementation of ammonium sulphate and urea on tofu whey highly supported the growth of bacterial population for about 6.5 × 108 CFU/mL and 5.4 × 108 CFU/ mL, respectively, but still lower compared to MRS media (2.03 × 1010 CFU/mL).

PEMANFAATAN BIOMASSA LIGNOSELULOSA AMPAS TEBU UNTUK PRODUKSI BIOETANOL Euis Hermiati; Djumali Mangunwidjaja; Titi Candra Sunarti; Ono Suparno; Bambang Prasetya
Jurnal Penelitian dan Pengembangan Pertanian Vol 29, No 4 (2010): Desember, 2010
Publisher : Badan Penelitian dan Pengembangan Pertanian

Utilization of lignocellulosic biomass from sugarcane bagasse for bioethanol production Sugarcane bagasse is one of potential lignocellulosic biomass for energy through physical, chemical or biological conversion. The material is renewable and abundantly available, especially as wastes or by-products of sugarcane industries. Of many conversion processes, lignocellulosic conversion to ethanol becomes focus of interest recently, since ethanol can be further used as biofuel to substitute gasoline for transportation. Lignocellulosic material, including sugarcane bagasse mainly consists of three components, namely cellulose, hemicellulose, and lignin. The conversion of these materials basically consists of pretreatment, cellulose hydrolysis, sugar fermentation to ethanol, and purification of ethanol. Production cost of this conversion is still high; therefore, many researches have been conducted to improve the conversion process, either pretreatment, hydrolysis, fermentation or purification, so that the cost could be reduced. This paper reviewed literatures on potential and characteristics of lignocellulosic materials, especially sugarcane bagasse, and conversion of these materials to ethanol. There is as much as 614,827 kL/year of ethanol potentially produced from sugarcane bagasse resulted from sugarcane factories in Indonesia. This amount of ethanol would have a great contribution to fulfill 1.10 million tons demand of ethanol. However, there are still some recalcitrans in production and implementation of lignocellulosic bioethanol, especially due to the unproven conversion technology of lignocellulosic biomass to ethanol and the high production cost. Therefore, government policies in supporting research and development, providing special incentives for sugarcane factories that produced ethanol from sugarcane bagasse, and giving subsidy to reduce bioethanol price, are needed.Keywords: Sugar by products, lignocellulose, conversion, ethanol

Optimization on Na and Ca bentonite activation using response surface method for increasing selectivity of stevioside in stevia extract Ayu Rahayu SARASWATI; Erliza NOOR; Titi Candra SUNARTI
E-Journal Menara Perkebunan Vol 89, No 2 (2021): Oktober, 2021
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v89i2.459

The demand for stevia extract consumption as an alternative sweetener that contains stevioside with zero-calorie is increasing. However, dark color and high tannin content from stevia leaf extract causes a disturbing aftertaste and reduces interest in consumption. Therefore, the purification process is a mandatory step to be done. Several studies on purification methods show the best approach by adsorption using bentonite. However, natural bentonite has limited adsorption capacity and low selectivity. An activation with acid and high temperature is expected to increase the adsorption capacity of color and its selectivity on maintaining the stevioside at the extract. This study aimed to obtain the optimum acid concentration and temperature for the activation using the Response Surface Method (RSM) experimental design and its application to the purification of stevia leaf extract. Based on the parameter of methylene blue number, the most optimum concentration of H2SO4 used for activation was 0.17 N for both natural bentonite. The heating temperature was 358°C for Na-bentonite and 481°C for Ca bentonite. The maximum adsorption capacities of activated Na and Ca-bentonite were increased from 15.65 and 38.23 mg g-1 to 197.72 and 169.52 mg g-1, respectively. The best adsorbent used for purification is Ca-activated, which increased extract clarification up to 81.37% at 655 nm and 86.64% at 410 nm compared to natural Ca bentonite. It also reduced tannin up to 97.46% and was more selective to recover 50.64% stevia content in the solution, which was higher than other previously reported studies.

Biodiversity of Cellulolytic Bacteria Isolated from the Solid Wastes of Agar Seaweed Processing Industry Ifah Munifah; Titi Candra Sunarti; Hari Eko Irianto; Anja Meryandini
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 10, No 3 (2015): December 2015
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v10i3.129

Agar is polysaccharide extracted from the seaweed Gracilaria sp. It is commonly used for food, medical, pharmaceutical and biotechnology industries. The red seaweed Gracilaria verrucosa is the most common material used for the extraction of agar, processed into sheet and powder. A total of 7169 tons solid waste is produced from the agar industry in Indonesia which can further result in 4301.4 tons per year of cellulose. However, solid wastes from agar seaweed processing industry have not been managed and used optimally, and therefore seaweed solid wastes can be used as one of alternative source of cellulose and cellulolytic bacteria. In this research, we reported the isolation of cellulose-producing bacteria from seaweed processing industry wastes using specific media 1 % LIA (Limbah Industri Agar, waste of seaweed processing industry). Sequencing and subsequent phylogenetic analysis of the 16S rRNA genes amplified from the obtained 7 isolates identified them as Serratia marcescens, Chryseobacterium indovlogenes, Bacillus pumilus, Bacillus cereus, Strenatrophomonas maltophila, and Pseudomonas aeruginosa.

Teknik Ekstraksi dan Nanoenkapsulasi Komponen Bioaktif Buah Malaka: Tinjauan Literatur Nida El Husna; Erliza Noor; Farah Fahma; Titi Candra Sunarti
AGROINTEK Vol 16, No 2 (2022)
Publisher : Agroindustrial Technology, University of Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrointek.v16i2.12433

The bioactive components of malacca (Emblica officinalis), which consist of phenolic, alkaloids, phytosterols, organic acids, and vitamins, are valuable for human health. The yield and quality of the bioactive components in the extract highly depend on the extraction technique, so it is essential to know the development of research on extraction techniques of the bioactive components of malacca fruit. In addition, the bioactive components in the extract have limitations in their application due to their stability, solubility, absorption, and bioavailability properties. Currently, nanoencapsulation technology has been applied to extracts of bioactive components to improve their properties. This review aims to provide comprehensive information about extraction techniques to obtain bioactive components of malacca fruit and presents the technique and purpose of the nanoencapsulation of malacca fruit extract. In addition to conventional techniques, several modern extraction techniques such as microwave-assisted extraction (MAE), ultrasound (UAE), pulsed electric field (PEF), and supercritical fluid (SFE) have been used to extract bioactive components of malacca fruit. Modern extraction techniques can produce extracts of bioactive components with higher yields than conventional techniques. The application of nanoencapsulation technology to malacca fruit extract consists of nanoliposomes and nanoparticles can increase permeability, antioxidant activity, antidiabetic and anti-inflammatory properties of malacca fruit extract.

The Antioxidant Effect of Nanoparticle Gels Grounds Arabica Coffee (Coffea arabica L.) Salfauqi Nurman; Ruka Yulia; Irmayanti Irmayanti; Erliza Noor; Titi Candra Sunarti
International Conference on Multidisciplinary Research Vol 3, No 1 (2020): ICMR
Publisher : Universitas Serambi Mekkah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32672/pic-mr.v3i1.2599

Arabica coffee’s grounds are the wastes from the production of coffee beverages and are known to contain several active compounds that can be used as an antioxidant. This research is therefore a follow-up work on the use of these coffee grounds nanoparticles as an active compound in gel production. This study focuses on the antioxidant effects of the gel product, using Box-Behnken Design with 3 factors(x) (carbopol 940, TEA, and coffee grounds nanoparticles) at 3 levels. Based on the results of this study, it is found that the high antioxidant effect with an average inhibition value was 43.485%.Furthermore, data analysis on the other hand showed a significant effect of linear Box-Behnken Design model on percentage inhibition with a p-value greater than 0.0001 and insignificant lack of fit validated by an F-value of 0.60, in addition to a p-value of 0.7621. The optimal solution of about 0.50% carbopol, 940 formulation, 0.40% TAE, and 2,313% nanoparticles produced 45,636% inhibition value with 0.812 desirability. Moreover, this arabica coffee grounds can be claimed as a new invention that has great potential in the health sector as an active compound for pharmaceutical preparations.Keywords: Inhibition, Nanogel, Coffee Grounds, Box-behnken

FORMULASI DAN UJI EFEK ANTIOKSIDAN GEL EKSTRAK AIR AMPAS KOPI ARABIKA (Coffea arabica L.) Salfauqi Nurman; Ruka Yulia; Irmayanti Irmayanti; Erliza Noor; Titi Candra Sunarti
Prosiding Seminar Nasional USM Vol 2, No 1 (2019): Semnas Multidisiplin Ilmu
Publisher : Universitas Serambi Mekkah

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2668.551 KB)

Senyawa antioksidan sangat dibutuhkan oleh tubuh untuk menangkal radikal bebas dari lingkungan, salah satu bahan alam yang mengadung senyawa antioksidan adalah ampas kopi arabika. Penelitian ini bertujuan untuk membuat formulasi gel antioksidan dari ekstrak air ampas kopi arabika dan menguji efektivitas antioksidan dari gel yang dihasilkan. Penelitian ini menggunakan Box-Behnken Design dengan 3 faktor (x) (carbopol 940, TEA dan ekstrak ampas kopi) dan 3 level untuk optimasi. % inhibisi yang dihasilkan oleh gel ekstrak ampas kopi arabika rata-rata adalah 32,650%. Model desain quadratic dipilih dalam penelitian karena berpengaruh signifikan terhadap % inhibisi dengan nilai p-value yaitu 0,0013 dan ketidaksignifikan lack of fit dengan F-value 1,24 dan nilai p-value 40,54%. Solusi optimal yang dihasilkan dari Box-Behnken Design adalah dengan formulasi carbopol 940 0,500 gram; TAE 0,600 mL; dan ekstrak 2,408 mL akan menghasilkan inhibisi 39,282% dengan desirability 0,965. Ampas kopi arabika memiliki potensi besar dalam bidang kesehatan sebagai sumber senyawa aktif dalam sedian farmasi.

Co-Authors Adisalamun Adisalamun Adriana, Savira Astri Adwiyani, Pustika Agus Setiyono AHMAD JUNAEDI Aisyah, Nadira Akhmad Endang Zainal Hasan Anas Miftah Fauzi Andi Nurul Aulia Sari Andika Susantri Ani Suryani Ani Suryani Ani Suryani Anja Meryandini Anja Meryandini Anja Meryandini Anja Meryandini Anja Meryandini Anja Meryandini Anja Meryandini Anja Meryandini Anuraga Jayanegara Anwar, Irvan Aris Purwanto Asep Wawan Permana Asep Wawan Permana, Asep Wawan Asrianti Basri Asrianti Basri Ayu Rahayu SARASWATI Bambang Prasetya Basri, Asrianti Basri, Asrianti Besty Maranatha Birahy, Deford Cristy Bruce A. Welt, Bruce A. Cahyana, Purwa Tri Christina Winarti Christina Winarti Dede Heri Yuli Yanto Dedi Fardiaz Devi Ambarwaty Oktavia Dewi Diniaty Djumali Mangunwidjaja Djumali Mangunwidjaja Djumali Mangunwidjaja Djumali Mangunwidjaja Djumali Mangunwidjaja Dwi Setyaningsih Efri Yulistika Eka Putri EKA RURIANI EKA RURIANI Eka Ruriani Elisa Julianti Elly Rosyidah Endang Warsiki Endang Warsiki Erliza Noor Euis Hermiati Evi Savitri Iriani Evi Savitri Iriani, Evi Savitri Faqih Udin FARAH FAHMA Fauzan, RM Muhammad Nur Februadi Bastian Feri Kusnandar Ferry Mutia Fiora Helmi Frangky J. Paat Glisina Dwinoor Rembulan Hadi Karia Purwadaria, Hadi Karia Hari Eko Irianto Hartami Dewi Hartrisari Hardjomidjojo Hasrul Satria Hasrul Satria, Hasrul Ifah Munifah Ika Amalia Kartika Ilham Marvie Indah Yuliasih IRA ERDIANDINI, IRA Irmayanti Irmayanti Irmayanti Irmayanti, Irmayanti Iskandar Lubis Kendri Wahyuningsih Kendri Wahyuningsih, Kendri Khaswar Syamsu Kirana Sanggrami Sasmitaloka Kurniawan Yuniarto Kurniawan, Dede Yudo Laily, Noer Lala Nurmala Machfud Machfud Mala Nurilmala Manalu, Lamhot Maranatha, Besty Marimin , Matasik, Delfania Maya Melati Mohamad Syamsul Ma’arif Mulyorini Rahayuningsih MUTIA, FERY NAOMI, APRILIA Nastiti Siswi Indrasti Nida El Husna Niken Financia Gusmawati NISA RACHMANIA MUBARIK Nisfatin Shofiana Nur Richana Nuri Andarwulan Odi Andanu Ono Suparno Paramuji, Muji Pasaribu, Fatimah J Petrus Adam Pramana, Yanuar Sigit Purwanto, Yanuar Jarwadi Purwoko Purwoko RAMADHAN, ZULFA AULIA Ratna Ekawati Ratna Ekawati Ridwansyah Ridwansyah Rijanti Rahaju Maulani Rijanti Rahaju Maulani Rijanti Rahaju Maulani Rini Purnawati Rismawati . Roh santoso Ronny Soputan Rosyidah, Elly Ruka Yulia SAFITRI NURLAELA Salfauqi Nurman, Salfauqi Sanbein, Primus Sapta Raharja Setyo Purwanto Singgih Wibowo Sitti Rahbiah Akram SOFIYANTO, M. EDY Stanislaus Aditya Agung Sugiyanta Sukard Sumarni Nompo Suprihatin Suprihatin Suprihatin Suprihatin Suprihatin Suprihatin Suprihatin Suryanegara, Lisman Suyono, Meisy Nawang Taufik Hidayat Tjahja Muhandri Tryanisa Ridla Amalia Utomo, Prasetyo Hadi Vestika Iskawati Wahidul Hijah Wahyu Widosari Wawan Hermawan Widiatmaka Widiatmaka Widosari, Wahyu WULAN, RAHAYU Yandra Arkeman Yaya Rukayadi Yuana Susmiati Yulia, Ruka YULIANA, META YULIN LESTARI Yunus Triyonggo, Yunus Yusmiati Yusmiati Zahrani, Siti Mutia

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