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All Journal Kolaboratif Bioscience Pendidikan Biologi Jurnal Biologi dan Pembelajarannya (JBP) Biomedika Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis Jurnal Agrotek Tropika JURNAL PENDIDIKAN TAMBUSAI Logista: Jurnal Ilmiah Pengabdian Kepada Masyarakat Acta Biochimica Indonesiana Eksakta : Berkala Ilmiah Bidang MIPA AL-ULUM: JURNAL SAINS DAN TEKNOLOGI Serambi Biologi Pelita Eksakta Jurnal Ilmu Pertanian Tirtayasa Sainstek : Jurnal Sains dan Teknologi Jurnal Biogenerasi Jurnal Biosense Symbiotic: Journal of Biological Education and Science Jurnal Serambi Biologi Jurnal Sains dan Kesehatan
Linda Advinda
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Universitas Negeri Padang, West Sumatera, Indonesia
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Specific primer design and optimization of annealing temperature for amplification gene peroxidase (POD) in Oryza sativa L. Nella Fauziah; Afifatul Achyar; Zulyusri Zulyusri; Yusni Atifah; Linda Advinda; Violita Violita
Bioscience Vol 7, No 2 (2023): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v7i2.122972

Abstract

Peroksidase (POD) merupakan enzim antioksidan yang memiliki beragam fungsi dalam siklus hidup tanaman, salah satunya adalah sebagai pertahanan dalam melawan ROS dengan mengkatalisis konversi H2O2 menjadi udara dan O2 . Kemampuan aktifitas enzim POD dalam mengatur kandungan H2O2 memungkinkan enzim tersebut dapat mempertahankan tanaman dari cekaman. Metode yang dapat digunakan untuk amplifikasi gen POD salah satunya yaitu kuantitatif reverse transcription- PCR (qRT-PCR). Metode ini memerlukan beberapa komponen penting salah satunya yaitu primer ( forward dan reverse ). Primer yang digunakan dalam amplifikasi gen harus spesifik terhadap gen target sehingga dapat mengenali dan menempel pada gen target yang diinginkan. Penelitian ini bertujuan untuk mendesain primer yang sesuai untuk amplifikasi gen POD menggunakan teknik qRT-PCR. Primer dirancang menggunakan perangkat PrimerQuest. Primer yang telah dirancang kemudian dianalisis untuk spesifikasinya dengan geneious prime . Kemudian spesifikasi primer di cek menggugakan primer BLAST. Hasil desain primer dengan kriteria terbaik untuk amplifikasi gen POD yaitu Forward POD 5'-AAATGCGTCGATCTACTGTACCT-3' dan Reverse POD 5'-GTGTTGAAAATGGCAATAAACCGG-3'
The Potential of Hot Water Sapan Sungai Aro Thermophilic Bacteria Consortium in Producing Bioethanol Inayatul Fatia; Irdawati Irdawati; Linda Advinda; Azwir Anhar
Bioscience Vol 7, No 2 (2023): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v7i2.123265

Abstract

Biofuel is a potentially renewable alternative fuel in Indonesia. Bioethanol is one example of the most commonly used biofuel. Microorganisms of thermophilic bacteria are known to contribute to the production of bioethanol. Thermophilic bacteria are efficient against high temperature conditions so as to minimize contamination. Production of bioethanol can also use joint culture (consortium). Bioethanol production using a microbial biculture consortium is known to significantly increase the level of bioethanol production. The purpose of this study was to determine the compatibility and to determine the optimum potential of the thermophilic bacterial biculture consortium of Sapan Sungai Aro hot water for bioethanol production. This research is a type of descriptive research. To test the cooperation between consortium isolates of thermophilic bacteria producing bioethanol, a compatibility test was carried out using the disk diffusion method. Then the consortium isolates were fermented in liquid TMM (Thermophilic Minimum Media) medium, the bioethanol content was measured after distillation using a pycnometer. The results of the bacterial compatibility test showed that there was one pair of isolates that were not compatible, namely SSA 8 & SSA 14 due to the presence of a clear zone. On research results. The production of bioethanol by a consortium of thermophilic bacteria gives more optimal results compared to a single isolate. The best thermophilic bacterial biculture consortium from the Sapan Sungai Aro hot spring in producing biofuels is SSA 14 & SSA 16 which is 3.009%. 
Jumlah Bakteri Pseudomonad Fluoresen Isolat Cas dalam Berbagai Formula Media Tumbuh Devi Aprillia Sary; Linda Advinda
Symbiotic: Journal of Biological Education and Science Vol. 2 No. 1 (2021): Symbiotic: Journal of Biological Education and Science Vol. 2 No. 1 April 2021
Publisher : Institut Agama Islam Negeri Kerinci

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32939/symbiotic.v2i1.24

Abstract

Fluorescent pseudomonad is a biological agent that can be used as a controlling plant disease, increasing plant growth and phosphate availability for plants, as well as producing compounds that are signals for plants to produce secondary metabolites that are antimicrobial. Pseudomonad fluorescent can be grown in various growing media. The purpose of the study was to find out the number of pseudomonad fluorescent bacteria in Cas isolates grown in various formulas. This research is an experimental study. The design used complete random design (RAL) with 5 treatments and 3 replays. The treatment is formula M1 (molasses 10 g/L+ ZA 5 g/L), M2 (molasses 10 g/L+ ZA 10 g/L), M3 (molasses 5 g/L+ ZA 5 g/L), M4 (molasses 5 g/L+ ZA 10 g/L), and M5 (NB 8 g/). The data obtained was analyzed with Anova and DNMRT dvanced tests at a real rate of 5%. The results showed that pseudomonad fluorescent isolate Cas grown in formula M3 ( molasses 5g/L + ZA 5 g/L) produced the highest number of bacteria which is 118.3 x 108 while the lowest number of bacteria in formula M5 ( NB 8g/L) is 9.76 x 108 cfu/mL.
The Potential of Hot Water Mudiak Sapan Thermophilic Bactery Consortium Formulation in Producing Xylanase Enzyme Irdawati; Indrawani Matondang; Linda Advinda; Azwir Anhar; Yusrizal Y
Jurnal Biologi Tropis Vol. 23 No. 4 (2023): October - December
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i4.5309

Abstract

The xylanase enzyme has high commercial value in the industrial sector, including the food industry, animal feed, bleaching of pulp/pulp, lignocellulosic bioconversion as fuel, and in the food industry, namely the cheese, bread and meat industries, while in the non-food industry this can be used in detergents. Xylanase enzymes can be produced from microorganisms, one of which is thermophilic bacteria. Thermophilic bacteria are known to be able to produce thermostable enzymes and proteins that are heat resistant. Xylanase production in compatible bacterial consortia was higher than monoculture. The purpose of this study was to determine the bicultur consortium that has the potential to produce xylanase enzymes. This research is an experimental research. Enzyme activity testing used the Miller method with DNS (Dinitrosalycilic acid) reagent using a completely randomized design (CRD) with 3 repetitions. Data on the results of xylanase activity were analyzed by ANOVA test and DMRT follow-up test with a 5% significance level.The results of the thermophilic bacterial isolates in the consortium that have the potential to produce xylanase enzymes are MS18 & MSS15,  MSS15, MS16 and MS18 & MS16.
The Ability of Fluorescent Pseudomonad to Produce Indole Acetic Acid (IAA) Maysyarah Ardiana; Linda Advinda
Jurnal Serambi Biologi Vol. 7 No. 1 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Fluorescent pseudomonads are a group of bacteria derived from the rhizosphere of plants. Fluorescent pseudomonads have potential as plant growth promoters capable of producing Indole Acetic Acid (IAA). This study aims to obtain fluorescent pseudomonad isolates that can produce IAA. The fluorescent pseudomonad isolates used were isolate Pf31 (derived from the rhizosphere of the longan plant), Pf32 (derived from the rhizosphere of the cassava plant), Pf33 (derived from the rhizosphere of the guava plant), Pf35 (derived from the rhizosphere of the galangal plant), Pf36 (derived from the rhizosphere of the guava plant). red ginger), Pf37 (derived from the rhizosphere of the Alocasia plant), Pf38 (derived from the rhizosphere of the celery plant), Pf39 (derived from the rhizosphere of the turmeric plant), and Pf40 (derived from the rhizosphere of the Oxalis plant). namely the detection of the ability of fluorescent pseudomonads to produce IAA. The results obtained showed that the fluorescent pseudomonads isolates Pf31, Pf32, Pf33, Pf35, Pf36, Pf37, Pf38, Pf39, and Pf40 had the ability to produce IAA with the highest concentration produced by isolate Pf36 which was 9.86 ppm and the lowest concentration was produced by isolate Pf38. ie 3.65 ppm.
Compatibility Test of Fluorescent Pseudomonad Isolated from Plant Rhizosphere Lati Jovanita; Linda Advinda
Jurnal Serambi Biologi Vol. 7 No. 1 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Fluorescent pseudomonads are a group of plant rhizosphere bacteria capable of producing antimicrobial compounds in the form of siderophores, HCN, decomposing phosphates and producing growth hormone Indole Acetic Acid (IAA). Fluorescent pseudomonads have various potentials as agents for controlling disease and increasing plant growth. Therefore, to maximize the potential of fluorescent pseudomonads, it is necessary to test compatible with each other. The aim of this study was to obtain compatible fluorescent pseudomonad isolates. This research is a descriptive study that informs the compatibility of fluorescent pseudomonad isolates isolated from plant rhizosphere. Compatibility test was carried out between the isolated fluorescent pseudomonad isolates. The results of this study obtained 6 combinations of compatible isolates: Pf1 x Pf8; Pf1 x Pf10; Pf7 x Pf9; Pf7 x Pf10; Pf8 x Pf9; and Pf9 x Pf10.
Effect of Fluorescent Pseudomonad Fluorescent Producing Indole Acetic Acid (IAA) Against Germination Red Chili (Capsicum annuum L.) Aditya Willy Putra; Linda Advinda
Jurnal Serambi Biologi Vol. 7 No. 1 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Red chili (Capsicum annuum L.) is an important horticultural commodity in Indonesia. Red chili propagation can be done by seed. Giving biological agents to seeds can accelerate the germination process. One of the biological agents capable of accelerating germination is fluorescent pseudomonad. Several species of pseudomonads are biological agents that can produce Indole Acetic Acid (IAA). This hormone plays a role in accelerating plant growth and development. The aim of this study was to examine the effect of fluorescent pseudomonads producing Indole Acetic Acid (IAA) on the germination of red chili (Capsicum annuum L.). This research is descriptive research and experimentation. The study design used was a complete randomized design (CRD) with 6 treatments (pf1, pf5, pf8, pf9 and pf10 isolates, and a aquades) and 3 repeats. The results showed that all the fluorescent pseudomonad isolates were able to produce IAA. The application of fluorescent pseudomonads did not significantly differ on germination, but significantly different on root length and stem length.
The Ability of Fluoresncent Pseudomonad to Produce Cyanide Acid Putri Andam Dewi; Linda Advinda
Jurnal Serambi Biologi Vol. 7 No. 1 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Fluorescent pseudomonads are a group of bacteria derived from plant rhizosphere and have the ability to produce antimicrobial compounds, namely cyanide acid. This study aims to obtain fluorescent pseudomonad isolates that can produce cyanide acid. The fluorescent pseudomonad isolates used were isolate Pf31 (derived from the rhizosphere of the longan plant), Pf32 (derived from the rhizosphere of the cassava plant), Pf33 (derived from the rhizosphere of the guava plant), Pf35 (derived from the rhizosphere of the galangal plant), Pf36 (derived from the rhizosphere of the guava plant). red ginger), Pf37 (derived from the rhizosphere of the Alocasia plant), Pf38 (derived from the rhizosphere of the celery plant), Pf39 (derived from the rhizosphere of the turmeric plant), and Pf40 (derived from the rhizosphere of the Oxalis plant). This research is a descriptive study conducted by detecting the ability of fluorescent pseudomonads to produce cyanide. The results of this study showed that 4 isolates produced cyanide, namely Pf31, Pf37, Pf38, and Pf39. Pf31 and Pf37 isolates produced the highest cyanide acid, indicated by a brick red color on the filter paper and Pf 32, Pf33, Pf35, Pf 36 and Pf40 isolates did not produce cyanide acid with the filter paper remaining yellow.
THE EFFECT OF VARIOUS CONCENTRATIONS OF ANTI-ACNE LIQUID SOAP ON THE BACTERIA OF Staphylococcus aureus CAUSES ACNE Delfi Suganda; Mades Fifendy; Linda Advinda
Jurnal Serambi Biologi Vol. 7 No. 4 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

his study aims to determine the effect of various concentrations of anti-acne liquid soap on Staphylococcus aureus and the interaction of the two types of liquid soap with their concentrations on the growth of S. aureus bacteria that causes acne. This research is an experimental study using a completely randomized design (CRD) in a 6 x 2 factorial with 3 replications. Factor A is liquid soap for acne, 2 levels are A1 = (Initial: P) , A2 = (Initial: C&C) Factor B is soap concentration, 6 levels are B1 = 2.5% (w/v), B2 = 5 % (w/v), B3 = 7.5% (w/v), B4 = 10% (w/v), B5 = 12.5% ​​(w/v), B6 ​​= Control (sterile distilled water). The results of this study indicate that the effect of various concentrations of anti-acne liquid soap on Staphylococcus aureus bacteria that causes acne is able to inhibit the growth of S. aureus, because the C&C brand of liquid anti-acne soap is able to form an average inhibition zone diameter of 1.94 cm at a concentration of 12, 5% while the anti acne P liquid soap brand was able to form an average inhibition zone diameter of 1.64 cm. From the statistical analysis data using the ANOVA test, the effect of various anti-acne liquid soaps on S.aureus had no significant effect and there was no interaction between the types of anti-acne liquid soap and its concentration on S.aureus which causes acne. Key words anti acne liquid soap, Staphylococcus aureus, concentration
Anti acne Solid Soap Antimicrobial Activity Test against Staphylococcus aureus Bacteria that Causes Acne Ade Indra Utama; Mades Fifendy; Linda Advinda
Jurnal Serambi Biologi Vol. 7 No. 1 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

The purpose of this study was to determine the antimicrobial activity of the anti-acne solid soap type and the effect of its concentration as well as the interaction between the anti-acne solid soap type and its concentration against Staphylococcus aureus bacteria that causes acne. This research was conducted from October - December 2021 at the Microbiology Laboratory, Faculty of Mathematics and Science, Universitas Negeri Padang. The methodology used in this study was an experiment with disc diffusion method using a completely randomized design (CRD) in a factorial consisting of 6 treatments and 3 repetitions with anti acne solid soap at concentrations of 2.5%, 5%, 7.5%, 10 %, 12.5% (w/v) and control (sterile distilled water). The inhibition zone diameter data were statistically analyzed using ANOVA, with = 5% and continued with Duncan's New Multiple Range Test (DNMRT) further test. The results of this study showed that both anti-acne solid soaps were able to provide antimicrobial activity against S. aureus bacteria at different concentrations because brand A and J solid anti-acne soap were able to form the highest average diameter of inhibition zones of 2.67 cm and 4, respectively. ,39 cm at 5% concentration. From the statistical analysis data using ANOVA, it was seen that the type and concentration of anti-acne solid soap had no significant effect on S. aureus bacteria and there was no interaction between the type of anti-acne solid soap and its concentration on S. aureus bacteria that causes acne.
Co-Authors A. Nabilla Nabilla Achyar, Afifatul Ade Indra Utama Aditya Willy Putra Ahmad Wibisana, Ahmad Aissa Febrya, Malika Alya Fariani Fariani Amelya Ningsih, Putri Andesmora, Evan Vria Andriani Andriani Aprilika, Kenny Arlina, Sistika Atifah, Yusni Atiqahh Muthiah Azwir Anhar Beni Gusnadi Betti Anggrayeni Chahya, Anisha Claudia Ayesha Dartiani Sagurung, Mariance Delfi Suganda Des M Devi Aprillia Sary Dezi Handayani Dhea Sabrina Aprilia Dilla, Aprina Dina Sukma Dwi Hilda Putri Elsa Badriyya Elsa Yuniarti Ernawati Ernawati Febri Doni Feby Yeriska Fevria, Resti Fitri Angraini Fitri Arsih Fitri, Ryantika Fitria, Davina Fronica, Imelda Ghiffari, Muhammad Handayani, Delsi Fitri Haqil Triyatdipa Hervi, Fauziah Huda Asra, Rahmatul Husnul Khatimah Idos Susila Ningsih Ilham Pratama Inayatul Fatia Indra Hartanto Indrawani Matondang Intan Febriani Irawati, Wike IRDAWATI Irdawati Irdawati Irdawati Irdawati Irdawati Irdawati Irma Leilani Irma Leilani Eka Putri Irma Leilani Eka Putri Jannah Koftiah Jumatul Hafsah Kiki Amelia, Kiki Larashinda, Mentari Lati Jovanita Listi Handayani M Maizirwan Mades Fifendy Mantoviana, Tiffany Marisa Marisa Marsha Utami Maysyarah Ardiana Milka Saputri Moralita Chatri Muhammad Deedat Ayasy Mukhlis Mukhlis Mulia Mulia Mulia Mulia, Nanta Mulyadi, Rival Nabilah, Rezi Nadia Nella Fauziah Niken Maharani Putri Nola Nurdianata Novi Novi Nur Vaizi Nurfa Dewiza Luzik Nurfatihah Z, Zahara Puspa Anggraeni Putri Putri Andam Dewi Putri, Santi Diana Rahmadhani Fitri Rahmawita Rahmawita Rahmawita Rahmawita Rahmi Holinesti Rahmi, Elva Ramadhan Sumarmin Resti Yulia Rusendra, Nabilla Makra S. Syamsurizal Salma Sakina Salsabilla, Vishtari Sari, Feby Djumaita sari, Mutia Selly Fildawati Silviana Okwisan Simbolon, Perima Siska Alicia Farma Situmorang, Rivaldo Sri Rahmadani Fitri Syahrastani, Syahrastani Syamsurizal Syamsurizal - - Syamsurizal Syamsurizal Vauzia, Vauzia Violita Violita Violita Violita Viona, Alda Wilna Sari Windi Nopitasari Yossi Rahmadeni Yulanda Saputri Yuni Ahda Yusrizal Y Yusrizal Y Yusrizal Yusrizal Zulyusri, Zulyusri