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All Journal Kolaboratif Bioscience Pendidikan Biologi Jurnal Biologi dan Pembelajarannya (JBP) Biomedika Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis Jurnal Agrotek Tropika JURNAL PENDIDIKAN TAMBUSAI Logista: Jurnal Ilmiah Pengabdian Kepada Masyarakat Acta Biochimica Indonesiana Eksakta : Berkala Ilmiah Bidang MIPA AL-ULUM: JURNAL SAINS DAN TEKNOLOGI Serambi Biologi Pelita Eksakta Jurnal Ilmu Pertanian Tirtayasa Sainstek : Jurnal Sains dan Teknologi Jurnal Biogenerasi Jurnal Biosense Symbiotic: Journal of Biological Education and Science Jurnal Serambi Biologi Jurnal Sains dan Kesehatan
Linda Advinda
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Universitas Negeri Padang, West Sumatera, Indonesia
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Fluorescent Pseudomonad Association in Plant Rhizosphere Intan Febriani; Linda Advinda; Dezi Handayani; Siska Alicia Farma; Dwi Hilda Putri
Jurnal Serambi Biologi Vol. 8 No. 2 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Plant growth promoting rhizobacteria or known as Plant Growth Promoting Rhizobacteria (PGPR) are bacteria found on the surface of plant roots and are beneficial microorganisms. The rhizosphere is an ideal area for the growth and development of soil microorganisms. This situation is supported by its function, namely as a provider of nutrients and also as a place for the growth and development of microorganisms. One of the bacteria found in the rhizosphere of plants is the fluorescent pseudomonad. Fluorescent pseudomonads are biological agents that can be isolated from the surface area of plant roots. This study aims to collect and analyze articles related to the association of fluorescent pseudomonads from plant rhizosphere. This type of research is research that uses the literature review method and literature collection using internet sources from the Google Scholar database. This stage consists of identification, screening, eligibility and acceptance. Based on the articles collected, it was found that the use of fluorescent pseudomonads as a biological control agent has been widely carried out because of their ability to produce antimicrobial compounds.
Pseudomonas fluorescens as Plant Growth Promoting Rhizobacteria (PGPR) Atiqahh Muthiah; Linda Advinda; Azwir Anhar; Irma Leilani Eka Putri; Siska Alicia Farma
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

The continuous use of chemical fertilizers will cause negative impacts such as pollution of the agricultural environment. This needs to be addressed by using Plant Growth Promoting Rhizobacteria (PGPR) which can trigger plant growth. Pseudomonas fluorescens is a bacterium that lives in the rhizosphere and is included in PGPR bacteria. P. fluorescens has the ability to produce siderophores, IAA, dissolve phosphate and fix nitrogen. Therefore, these bacteria can increase growth, provide plant nutrients and inhibit pathogens that cause plant diseases. This study aims to collect and analyze articles related to Pseudomonas fluorescens as PGPR. This type of research is research that uses the literature review method and literature collection using internet sources from the Google Scholar database. This stage consists of identification, screening, eligibility and acceptance. Based on the articles collected, it was found that P. fluorescens as PGPR can increase plant growth by having the ability to produce IAA, dissolve phosphate, produce siderophores and fix nitrogen.
Pseudomonas fluorescens as a Biocontrol Agent for Controlling Various Plant Diseases Beni Gusnadi; Linda Advinda; Azwir Anhar; Irma Leilani Eka Putri; Moralita Chatri
Jurnal Serambi Biologi Vol. 8 No. 2 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Biological control or biocontrol is a way that involves organisms to reduce the growth of pathogens and reduce the impact of disease. The use of biocontrol agents is an alternative to control plant disease-causing pathogens. One of the biocontrol agents that can be used is Pseudomonas fluorescens. Basically P. fluorescens is the largest group producing antibiotics and antimicrobial compounds of cyanide acid (HCN) and siderophores. The compounds it produces can inhibit activity and control pathogens. Therefore, P. fluorescens has great potential as a biocontrol agent for controlling plant diseases. This study aims to collect and analyze articles related to P. fluorescens as a biocontrol agent for controlling various plant diseases. This type of research is research that uses internet sources from the Google Scholar database. The stages consist of identification, screening, eligibility and acceptance. Based on the articles collected, it was found that P. fluorescens is a biocontrol agent that can control various plant diseases. Biocontrol agents influence the activity and growth of pathogens thanks to the compounds they produce
Saponins Compounds as Antifungal Against Plant Pathogens Resti Yulia; Moralita Chatri; Linda Advinda; Dezi Handayani
Jurnal Serambi Biologi Vol. 8 No. 2 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Saponin merupakan salah satu senyawa metabolit sekunder yang terkandung dalam tanaman. Saponin tersebar luas pada seluruh organ tanaman diantaranya yaitu pada buah, biji, daun, umbi, batang, dan akar. Kandungan saponin lebih banyak ditemukan pada tanaman yang masih muda dari pada tanaman berumur tua. Metode yang digunakan yaitu studi literature. Ulasan ini bertujuan untuk memberikan gambaran mengenai efektivitas saponin sebagai antifungi. Saponin dapat diperoleh dari tanaman dengan metode ekstraksi. Metode ekstraksi merupakan sebuah proses pemisahaan berdasarkan perbedaan kelarutan bahan. Mekanisme kerja saponin sebagai antifungi yaitu dengan cara menurunkan membrane sterol sehingga mengakibatkan permeabilitas meningkat, lalu sel bengkak dan pecah sehingga mengakibatkan sel menjadi mati. Matinya sel tersebut mengakibatkan pertumbuhan dan perkembangan jamur menjadi terhambat. Dari beberapa penelitian telah terbukti bahwa saponin yang terdapat dalam tanaman terbukti memiliki aktivitas antifungi sehingga dapat dijadikan sebagai fungisida nabati.Kata Kunci: antifungi, tanaman, saponin, sel, jamur
Endophytic Fungi from Simpei Fern Rhizomes and Its Phosphate Solubilization Activity Windi Nopitasari; Dezi Handayani; Linda Advinda; Irdawati Irdawati
Jurnal Serambi Biologi Vol. 8 No. 2 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Phosphate is very abundant in the soil, but only a small amount was dissolved. Some fungi have the ability to dissolve phosphates. This fungi is expected to overcome the excessive use of phosphate fertilizers. Those fungi ussualy found in soil or rhizorfere and sometimes they are endophytic. Rhizome of the simpei fern has endophytic fungi and its phosphate solubilizing activity was not known yet. This study aims to isolate endophytic fungi from rhizome of the simpei fern and to determine its phosphate solubilizing activity. Samples of simpei fern rhizome were taken from Nagari Aka Barayun, Harau District, Lima Puluh Kota District, West Sumatra. Surface sterilization using 0.5% NaOCL. The isolation medium for endophytic fungi used PDA medium and the phosphate solvent activity test used Pikovskaya medium. Six isolates of endophytic fungi were successfully isolated from the rhizome of the simpei fern and only three isolates were able to dissolve phosphate. The isolates are isolates R4, R5, and R6. Phosphate solubilizing activity of isolate R5 was higher than other isolates, although all isolates had a low phosphate solubility index.
Senyawa Aktif Flavonoid yang Terdapat Pada Tumbuhan Idos Susila Ningsih; moralita Chatri; Linda Advinda
Jurnal Serambi Biologi Vol. 8 No. 2 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Metabolit sekunder merupakan komponen kimia yang dihasilkan tumbuhan melalui biosintesis senyawa metabolit primer. Metabolit sekunder memiliki berbagai macam fungsi salah satunya sebagai pertahanan terhadap patogen. Senyawa metabolit sekunder yang umum terdapat pada tumbuhan adalah flavonoid, alkaloid, saponin, fenol, tanin, steroid, dan triterpenoid. Flavonoid merupakan kelompok polifenol dan diklasifikasikan berdasarkan struktur kimia serta biosintesisnya. Flavonoid mempunyai kerangka dasar karbon yang terdiri dari 15 atom karbon. Dimana dua cincin benzena (C6) terikat oleh rantai propana (C3). Flavonoid memiliki beberapa bentuk seperti isoflavon, flavonol dan flavon, dan flavanon. Flavonoid ini memiliki berbagai macam funfsi seperti sebagai antioksidan, antiinflamasi, antibakteri dan antijamur. Kata kunci: Flavonoid, Antioksidan, Antijamur, Antiinflamasi, Senyawa metabolit sekunder
Karakteristik Saponin Senyawa Metabolit Sekunder pada Tumbuhan Puspa Anggraeni Putri; Moralita Chatri; Linda Advinda
Jurnal Serambi Biologi Vol. 8 No. 2 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Senyawa kimia dalam tumbuhan merupakan hasil metabolisme sekunder dari tumbuhan itu sendiri. Senyawa metabolit sekunder yaitu senyawa organik yang dihasilkan tumbuhan tetapi tidak memiliki fungsi langsung pada fotosintesis, pertumbuhan atau respirasi, transportasi zat terlarut, translokasi, sintesis protein, asimilasi nutrien, diferensiasi, pembentukan karbohidrat, protein dan lipid. Senyawa metabolit sekunder ini dikelompokkan menjadi beberapa golongan berdasarkan stuktur kimianya, salah satunya yakni saponin. Istilah saponin diturunkan dari bahasa Latin “sapo” yang berarti sabun, diambil dari kata Saponaria vaccaria, suatu tumbuhan yang mengandung saponin digunakan sebagai sabun untuk mencuci. Pada tumbuhan, saponin tersebar merata dalam bagian-bagiannya seperti akar, batang, umbi, daun, biji dan buah. Struktur kimia saponin merupakan glikosida yang tersusun atas glikon dan aglikon. Saponin merupakan senyawa glikosida kompleks dengan berat molekul tingi yang dihasilkan terutama oleh tumbuhan, hewan laut tingkat rendah dan beberapa bakteri. Saponin dapat menurunkan tegangan permukaan air, sehingga akan mengakibatkan terbentuknya buih pada permukaan air setelah dikocok. Sifat ini mempunyai kesamaan dengan surfaktan. Rasa saponin sangat ekstrim, dari sangat pahit hingga sangat manis dan merupakan senyawa ampifilik. Saponin dapat digunakan sebagai pembasmi hama udang, sebagai deterjen, sebagai pembentuk busa pada sampo, dan dapat menghambat jamur dan melindungi tumbuhan dari serangan serangga. Saponin dapat menjadi antijamur dengan cara menurunkan tegangan permukaan dinding sel dan merusak permeabilitas membran. Saponin berdifusi melalui membran luar dan dinding sel yang rentan kemudian mengikat membran sitoplasma sehingga mengganggu dan mengurangi kestabilan membran sel.
ANALYSIS SPECIFIC ACTIVITY OF XYLANASE ENZYME PRODUCED BY THE CONSORTIUM OF THERMOPHILIC BACTERIA FROM SAPAN SUNGAI ARO HOT SPRING Feby Yeriska; Mulia; Irdawati; Linda Advinda; Dwi Hilda Putri
Jurnal Serambi Biologi Vol. 8 No. 3 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Thermophilic bacteria are bacteria that are able to survive in high temperatures ranging from 45 – 80oC so that they have the potential to produce enzymes that are stable against heat. Thermophilic bacteria can be found in various places in nature that have potential hot springs. One of the potentials of thermophilic bacteria in producing enzymes is the xylanase enzyme. Xylanase is able to hydrolyze xylan (hemicellulose) into xylo-olisaccharides and xylose. In the industrial world, xylanase enzymes are used as paper whiteners, increase the volume of bread, as juice purification, and are used in the manufacture of detergents. This research is a type of descriptive research that aims to determine the specific activity of the xylanase enzyme and the levels of xylanase protein produced by thermophilic bacteria SSA (Sapan Sungai Aro) with bacterial isolates SSA3, SSA4, SSA5, SSA6, SSA7, SSA8, SSA13, SSA14 and SSA15. The results showed that the bacterial isolates SSA3, SSA4, SSA5; isolates SSA6, SSA7, and SSA8; and SSA13, SSA14 and SSA15 respectively had specific activity of the xylanase enzyme with an average value of 0.1614 Unit/mg; 0.1638 Unit/mg and 0.1473 Unit/mg. The final measurement results showed that the highest specific activity of the xylanase enzyme was in the consortium of thermophilic bacteria isolates SSA6, SSA7, and SSA8 with an average of 0.1638 Unit/mg.
Specific Primer Design and Optimization of Monodehydroascorbate reductase (MDHAR) Gene Amplification in Rice (Oryza sativa L.) Jumatul Hafsah; Afifatul Achyar; Zulyusri; Yusni Atifah; Linda Advinda; Violita
Jurnal Serambi Biologi Vol. 8 No. 3 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Monodehydroascorbate reductase (MDHAR) is an enzyme responsible for growth and response to biotic and abiotic stress. MDHAR in rice shows a higher sensitivity to stress compared to other plants. This study aims to obtain specific primers for the MDHAR gene in rice to be used in PCR amplification so that it can amplify the MDHAR gene. Primers are designed using the Pickprimer and Geneious Primer tools. Optimization of annealing temperature was carried out using the gradient PCR method and then an in vitro primary specification test was carried out using the Touchdown PCR method. The results of the primary design obtained one candidate primer that met the ideal primer requirements, namely a pair of primers (5'-AAAAACACTGCATGGGTCGTC-3' and 5'-CGCCTACCGTTTCCCAAGTT-3') with an amplicon length of 160 bp. The visualization results of PCR products using 1.5% agarose showed that 6 samples were able to amplify the MDHAR gene at 160 bp in size. However, in each lane there is a non-specific DNA band (Primer dimer). In vitro primer specification testing with Touchdown succeeded in increasing product formation specifications and was able to reduce non-specific DNA bands (Primer dimers).
PRODUCTION OF SPESIFIC XYLANASE ENZYME BY MUDIAK SAPAN HOT WATER THERMOPHILIC BACTERY Irdawati Irdawati; Muhammad Deedat Ayasy; Azwir Anhar; Linda Advinda; Yusrizal Yusrizal
Bioscience Vol 8, No 1 (2024): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v8i1.122728

Abstract

Xylanase is an extracellular enzyme capable of hydrolyzing hemicellulose so that it can convert xylan into xylose. Thermostable xylanase enzymes can be produced by thermophilic bacteria. Thermophilic bacteria are used because they have advantages such as the ability to increase enzyme production in adjustable catalytic specifications. Bacteria are not only in a single form but also exist in a mixed form called a consortium. Compared with a single isolate, the performance of the consortium is better. Consortium is a mixture of microbial populations in the form of communities that have cooperative, commensal, and mutualistic relationships. This study aims to look at the cooperation between the consortium isolates and the consortium's ability to produce xylanase enzymes. This research is a descriptive study. The bacterial consortium is fermented in beechwood xylan medium. The results of this study were that MSS 11, MS 18, MS 16 consortium produced the highest xylanase enzyme activity, namely 12,887.Xilanase merupakan enzim ekstraseuler yang mampu menghidrolisis hemiselulosa sehingga dapat merubah xilan menjadi xilosa. Enzim xilanase termostabil dapat diproduksi oleh bakteri termofilik. Bakteri termofilik digunakan karena dapat memiliki keunggulan seperti aktivitas dan peningkatan spesifikasi katalisis yang dapat diatur. bakteri tidak hanya dalam bentuk tunggal namun juga ada dalam bentuk campuran yang disebut konsorsium. Dibandingkan dengan isolat tunggal, kinerja konsorsium lebih baik. Konsorsium merupakan campuran populasi mikroba dalambentuk komunitas yang mempunyai hubungan kooperatif, komensal, dan mutualitsik. Penelitian ini bertujuan untuk melihat kerjasama antar isolat konsorsium dan kemampuan konsorsium dalam menghasilkan enzim xylanase. Penelitian ini bersifat deskriptif. Pada penelitian ini, konsorsium bakteri difermentasikan dalam medium beechwood xylan. Hasil penelitian ini adalah konsorsium MSS 11, MS 18, MS 16 menghasilkan aktivitas enzim xylanase spesifik tertinggi yaitu 12,887.
Co-Authors A. Nabilla Nabilla Achyar, Afifatul Ade Indra Utama Aditya Willy Putra Ahmad Wibisana, Ahmad Aissa Febrya, Malika Alya Fariani Fariani Amelya Ningsih, Putri Andesmora, Evan Vria Andriani Andriani Aprilika, Kenny Arlina, Sistika Atifah, Yusni Atiqahh Muthiah Aulia, Fadila Azwir Anhar Beni Gusnadi Betti Anggrayeni Chahya, Anisha Claudia Ayesha Dartiani Sagurung, Mariance Delfi Suganda Des M Devi Aprillia Sary Dezi Handayani Dhea Sabrina Aprilia Dilla, Aprina Dina Sukma Dwi Hilda Putri Eka Nuraini, Fauzi Elsa Badriyya Elsa Yuniarti Ernawati Ernawati Febri Doni Febrianti, Liza Feby Yeriska Fevria, Resti Fitri Angraini Fitri Arsih Fitri, Ryantika Fitria, Davina Fronica, Imelda Ghiffari, Muhammad Hamdah, Alya Handayani, Delsi Fitri Haqil Triyatdipa Hervi, Fauziah Huda Asra, Rahmatul Husnul Khatimah Idos Susila Ningsih Ilham Pratama Inayatul Fatia Indra Hartanto Indrawani Matondang Intan Febriani Irawati, Wike IRDAWATI Irdawati Irdawati Irma Leilani Irma Leilani Eka Putri Irma Leilani Eka Putri Jannah Koftiah Jumatul Hafsah Kiki Amelia Kiki Amelia, Kiki Larashinda, Mentari Lati Jovanita Listi Handayani M Maizirwan Mades Fifendy Mantoviana, Tiffany Mardhiyah Nazri, Laila Marisa Marisa Marsha Utami Maysyarah Ardiana Mentari Larashinda Milka Saputri Moralita Chatri Muhammad Deedat Ayasy Mukhlis Mukhlis Mulia Mulia Mulia Mulia, Nanta Mulyadi, Rival Nabilah, Rezi Nadia Nazri, Laila Mardhiyah Nella Fauziah Niken Maharani Putri Nola Nurdianata Norman, Mayanda Novi Novi Nur Vaizi Nuraini, Fauzi Eka Nurfa Dewiza Luzik Nurfatihah Z, Zahara Pratama Putra, Abil Puspa Anggraeni Putri Putri Andam Dewi Putri, Santi Diana Rahmadhani Fitri Rahmawita Rahmawita Rahmawita Rahmawita Rahmi Holinesti Rahmi, Elva Ramadhan Sumarmin Resti Yulia Rusendra, Nabilla Makra S. Syamsurizal Salma Sakina Salsabilla, Vishtari Sari, Feby Djumaita sari, Mutia Selly Fildawati Silviana Okwisan Simbolon, Perima Siska Alicia Farma Situmorang, Rivaldo Sri Rahmadani Fitri Syahrastani, Syahrastani Syamsurizal Syamsurizal - - Syamsurizal Syamsurizal Taufiqqurrahman Taufiqqurrahman Vauzia, Vauzia Violita Violita Violita Violita Viona, Alda Wilna Sari Wilna Sari Winardi, Fhuji Windi Nopitasari Wulandari, Tesya Yossi Rahmadeni Yulanda Saputri Yuni Ahda Yunis Marni Yusrizal Y Yusrizal Yusrizal Yusrizal Yusrizal Zen, Muhammad Nazif Zulyusri, Zulyusri