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Antimicrobic Activities Of Solid Soap Against Staphylococcus aureus Dan Escherichia coli Human Pathogen Bacteria A. Nabilla Nabilla; Linda Advinda
Jurnal Serambi Biologi Vol. 7 No. 4 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Staphylococcus aureus and Escherichia coli are the two types of bacteria that most often attack the human body. Solid bath soap is a soap that is used to clean the body and can function as an antimicrobial if the soap contains antimicrobial ingredients. This study aims to determine the antimicrobial activity of solid bath soap against S. aureus and E. coli bacteria. This research is an experimental study using a completely randomized design (CRD) with 5 treatments, namely A1 (initials: Lx), A2 (initials: Lb), A3 (initials: Gv), A4 (initials: Ct), and A5 (initials: Sz), with 3 repetitions. The method used is the disc diffusion method using a 5 mm diameter paper disc to determine the antimicrobial activity of soap and see the inhibition zone formed in the test medium. The results obtained showed that the antimicrobial activity of solid bath soap had no significant effect on the growth of S. aureus and E. coli bacteria. The highest antimicrobial activity in inhibiting the growth of S. aureus was A2 soap with an inhibition zone diameter of 22,27 mm, and the lowest was A1 soap with an inhibition zone diameter of 12,4 mm. While the highest inhibiting E. coli was A1 soap with an inhibition zone diameter of 20,17 mm, and the lowest was A5 soap with an inhibition zone diameter of 14,37 mm.
Pengaruh Berbagai Konsentrasi Sabun Padat Antiseptik Terhadap Escherichia coli Alya Fariani Fariani; Linda Advinda
Jurnal Serambi Biologi Vol. 7 No. 3 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi sabun padat antiseptik A1 (merek S), A2 (merek N), dan A3 (merek D) terhadap bakteri E. coli. Penelitian ini adalah penelitian eksperimen yang dilakukan dengan metode difusi cakram dengan menggunakan kertas cakram diameter 5 mm untuk mengetahui pengaruh konsentrasi sabun dan melihat zona hambat yang terbentuk di media uji. Hasil yang diperoleh menunjukkan terbentukya zona hambat A3 (merek D) yang paling tinggi terhadap E. coli dengan rerata sebesar 21,9 mm, sabun A1 (merek S) memiliki zona hambat terhadap E. coli dengan rerata sebesar 21,6 mm sedangkan, sabun A2 (merek N) memiliki zona hambat yang paling rendah terhadap E. coli dengan rerata sebesar 13,8 mm. Sabun A1 (merek S) dan A3 (merek D) menunjukkan hasil yang tidak berbeda nyata dengan sabun A2 (merek N). Sedangkan sabun A2 (merek N) berbeda nyata dengan sabun A1 (merek S) dan A3 (merek D). Dari hasil tersebut sabun A3 terbukti yang paling efektif dalam membunuh dan menghambat pertumbuhan bakteri E. coli.
Effect of Sungkai Leaf Extract (Peronema canescens J.) on Colony Diameter and Percentage of Growth of Inhibition Fusarium oxysporum Marisa Marisa; Moralita Chatri; Linda Advinda; Mades Fifendy
Jurnal Serambi Biologi Vol. 7 No. 3 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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The purpose of this study was to determine the effect of the concentration of leaf extract P. canescens on colony diameter and the percentage of inhibition of growth of F. oxysporum. This research was conducted from November 2021 to December 2021, at the Integrated Research Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, Padang State University. This study was an experimental study consisting of 5 treatments and 3 replications with leaf extract at P. canescens concentrations of 0%, 10%, 20%, 30%, 40%. The data obtained were analyzed by means of variance (ANOVA) with Duncan's New Multiple Range Test (DNMRT) further test. The results showed that the leaf extract of P. canescens . Effect on the diameter of the fungal colonies of F. oxysporum. The concentration of leaf extract affected the percentage of growth of inhibition F.oxysporum. The lowest percentage of inhibition was at a concentration of 10% and the highest at a concentration of 40%.
The Response Of The Growth Of Robusta Coffee (Coffea Canephora. L) That Received Treatment Of Time Of Immersion And Concentration Of Sulphic Acid (H2SO4) Sri Rahmadani Fitri; Azwir Anhar; Linda Advinda; Violita Violita
Jurnal Serambi Biologi Vol. 7 No. 4 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Robusta coffee seeds (Coffea canephora L.) require dormancy breaking treatment to accelerate germination. One of the treatments for breaking dormancy that can be done is chemical scarification by adjusting the concentration and duration of sulfuric acid (H2SO4) immersion. This study aims to determine the best concentration (H2SO4) and soaking time for breaking dormancy of robusta coffee seeds. This research was conducted in the Biology laboratory of the Faculty of Mathematics and Natural Sciences, Padang State University, in November-January 2022 using a two-factor completely randomized design. The first factor is the concentration of H2SO4 solution (0%, 10%, 15% and 20%). The second factor is the immersion time (25 minutes, and 50 minutes). Each treatment combination consisted of 4 replications. Parameters observed were germination percentage, germination rate, vigor index, hypocotyl length (cm), root length (cm), and dry weight (grams). The results showed that the H2SO4 concentration treatment had a very significant effect on the percentage of germination parameters, and dry weight while the other parameters had no significant effect. The treatment duration of immersion had a significant effect on the parameters of germination percentage, germination rate, and dry weight while the other parameters had no significant effect. The combination treatment between H2SO4 concentration and soaking time had a very significant effect on the germination rate parameter, significantly affected the germination percentage parameter, and had no significant effect on other parameters.
The Effect Of Ecoenzyme Spraying On The Number Of Leaves Of Spinach (Amaranthus hybridus L.) Cultivated Hydroponically. Dhea Sabrina Aprilia; Resti Fevria; Vauzia; Linda Advinda
Jurnal Serambi Biologi Vol. 7 No. 3 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Spinach is one of the vegetables favored by the people of Indonesia and also has high nutritional value. Spinach can be cultivated conventionally or hydroponically. Hydroponics is one solution to the problem of limited land in farming that uses water as a nutrient medium that will be directly absorbed by plants to support plant growth. Hydroponics uses Ab-Mix nutrients. To get better results, ecoenzyme can be used as a support for plant fertilizers. This study aims to see the effect of spraying ecoenzyme on the number of spinach leaves. This study is an experimental study consisting of 5 treatments and 5 replications with a dilution of ecoenzyme solution, P1 (control), P2 (2mL ecoenzyme + 1L water), P3 (3mL ecoenzyme + 1L water), P4 (4mL ecoenzyme + 1L water), and P5 (5mL ecoenzyme + 1L water). The data obtained were analyzed by means of variance (ANOVA) with Duncan's New Multiple Range Test (DNMRT) further test. The results showed that spraying ecoenzyme on spinach plants had no effect on the number of plant leaves.
The Effectiveness Of Noni Leaf Extract (Morinda citrifolia L.) As Antifungal Against The Growth Of Sclerotium rolfsii In Vitro Marsha Utami; Linda Advinda; Violita Violita; Moralita Chatri
Jurnal Serambi Biologi Vol. 7 No. 2 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Pathogens can cause disease in plants such as stem rot, wilting and germination are the fungus Sclerotium rolfsii. S. rolfsii fungus control using synthetic fungicides. However, synthetic fungicides can cause negative impacts such as environmental pollution. Another alternative can be used is a plant-based fungicide. Plants can be used as vegetable fungicides, namely noni leaves (Morinda citrifolia L.) contain several antimicrobial compounds such as flavonoids, alkaloids and steroids that can inhibit fungal growth. The purpose of this study was to test the effectiveness and antifungal activity of noni leaf extract in inhibiting the growth of S. rolfsii. This type of research is an experimental research with 5 treatments and 3 replications which will be carried out in November-December 2021 at the Research Laboratory of the Biology Department, FMIPA UNP. The treatments given were: 0% concentration (control), 10%, 20%, 30% and 40%. The antifungal activity was analyzed descriptively. The results showed that noni leaf extract was effective in inhibiting the growth of the fungus S. rolfsii and all treatments were significantly different from the control. The antifungal activity of noni leaf extract against S. rolfsii at concentrations of 10%, 20% and 30% was weak, while the concentration of 40% was moderate.
Seed Biopriming Using Plant Growth Promoting Rhizobacteria (PGPR) Milka Saputri; Linda Advinda; Azwir Anhar; Violita; Moralita Chatri
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Rhizobacteria are bacteria found on the surface of plant roots and are able to increase plant growth known as Plant Growth Promoting Rhizobacteria (PGPR). Rhizobakteri can be used for plant germination by biopriming. Biopriming is hydration of seeds in a controlled manner using biological compounds. Several species of plant growth promoting rhizobacteria that can be used for biopriming are Pseudomonas fluorescens, P. putida and Bacillus subtilis. Biopriming is an alternative method for controlling seeds from soil-borne pathogens and can help farmers use microbes to apply the crops they want. From several research results, biopriming has been proven to increase plant productivity.
Formulation of Pseudomonad Fluorescent as Plant Growth Promoting Rhizobacteria Haqil Triyatdipa; Linda Advinda; Azwir Anhar; Dezi Handayani; Irdawati
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Plant growth promoting rhizobacteria, known as Plant Growth Promoting Rhizobacteria (PGPR), are bacteria found on the surface of plant roots and are beneficial microorganisms. Fluorescent pseudomonads are a group of bacteria belonging to PGPR and have the ability to dissolve phosphates, produce nitrogen, phosphorus, siderophores, and Indole Acetic Acid (IAA) to stimulate plant growth. PGPR must be immobilized in a certain carrier in the form of a formulation so that it is easy to use, store, commercialize, and apply to the field. Due to the increasingly expensive instant media, the development of media formulations continues to be carried out which can be organic or inorganic.
Potential Of Pseudomonas fluorescens As Plant Growth Promoting Bacteria Claudia Ayesha; Linda Advinda; Violita Violita; Dezi Handayani
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Plant growth promoting bacteria or Plant Growth Promoting Rhizobacteria (PGPR) are soil bacteria found in the root area which function to increase plant growth. Most of them came from the Gram-negative group with the highest number of strains from the genus Pseudomonas. Pseudomonas fluorescens is able to produce IAA, siderophores and phosphate solubilizing compounds, fixes nitrogen and exhibits plant growth promoting activity. Therefore, this bacterium is very potential as a PGPR. This study aims to collect and analyze articles related to the potential of Pseudomonas fluorescens as a plant growth promoting bacterium. This type of research is research that uses internet sources from the Google Scholar database. The stages consist of identification, screening, eligibility and acceptance. Based on the articles collected, it was found that Pseudomoas fluorescens is one of the Plant Growth Promoting Rhizobacteria (PGPR). PGPR affects plant health and soil fertility by offering an excellent combination of properties that are useful in promoting plant growth, namely producing IAA and siderophores, dissolving phosphate, fixing nitrogen and exhibiting plant growth promoting activity.
Potential of Pseudomonad Fluoresen as Control of Plant Diseases Silviana Okwisan; Linda Advinda; Dezi Handayani; Dwi Hilda Putri; Irma Leilani Eka Putri
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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The use of fertilizers and pesticides provides various side effects such as environmental pollution and higher production costs due to the high prices of fertilizers and pesticides. Therefore it is necessary to look for other alternatives to control plant diseases. One of the safe and environmentally friendly control measures is to use biological control agents. Fluorescent pseudomonads are antagonistic bacteria that are widely used as biological agents for fungi, pathogenic bacteria and viruses. Biological control using fluorescent pseudomonads has been studied for its effectiveness in suppressing plant diseases. This study aims to collect and analyze articles related to the potential of fluorescent pseudomonads in controlling plant diseases. This type of research is research that uses the literature review method and collects literature using internet sources from the Google Scholar database. The stages consist of identification, screening, eligibility and acceptance. Based on the articles collected, it was found that fluorescent pseudomonads are potential and effective in controlling various plant diseases.
Co-Authors A. Nabilla Nabilla Achyar, Afifatul Ade Indra Utama Aditya Willy Putra Ahmad Wibisana, Ahmad Aissa Febrya, Malika Alya Fariani Fariani Amelya Ningsih, Putri Andesmora, Evan Vria Andriani Andriani Aprilika, Kenny Arlina, Sistika Atifah, Yusni Atiqahh Muthiah Azwir Anhar Beni Gusnadi Betti Anggrayeni Chahya, Anisha Claudia Ayesha Dartiani Sagurung, Mariance Delfi Suganda Des M Devi Aprillia Sary Dezi Handayani Dhea Sabrina Aprilia Dilla, Aprina Dina Sukma Dwi Hilda Putri Elsa Badriyya Elsa Yuniarti Ernawati Ernawati Febri Doni Feby Yeriska Fevria, Resti Fitri Angraini Fitri Arsih Fitri, Ryantika Fitria, Davina Fronica, Imelda Ghiffari, Muhammad Handayani, Delsi Fitri Haqil Triyatdipa Hervi, Fauziah Huda Asra, Rahmatul Husnul Khatimah Idos Susila Ningsih Ilham Pratama Inayatul Fatia Indra Hartanto Indrawani Matondang Intan Febriani Irawati, Wike IRDAWATI Irdawati Irdawati Irdawati Irdawati Irdawati Irdawati Irma Leilani Irma Leilani Eka Putri Irma Leilani Eka Putri Jannah Koftiah Jumatul Hafsah Kiki Amelia, Kiki Larashinda, Mentari Lati Jovanita Listi Handayani M Maizirwan Mades Fifendy Mantoviana, Tiffany Marisa Marisa Marsha Utami Maysyarah Ardiana Milka Saputri Moralita Chatri Muhammad Deedat Ayasy Mukhlis Mukhlis Mulia Mulia Mulia Mulia, Nanta Mulyadi, Rival Nabilah, Rezi Nadia Nella Fauziah Niken Maharani Putri Nola Nurdianata Novi Novi Nur Vaizi Nurfa Dewiza Luzik Nurfatihah Z, Zahara Puspa Anggraeni Putri Putri Andam Dewi Putri, Santi Diana Rahmadhani Fitri Rahmawita Rahmawita Rahmawita Rahmawita Rahmi Holinesti Rahmi, Elva Ramadhan Sumarmin Resti Yulia Rusendra, Nabilla Makra S. Syamsurizal Salma Sakina Salsabilla, Vishtari Sari, Feby Djumaita sari, Mutia Selly Fildawati Silviana Okwisan Simbolon, Perima Siska Alicia Farma Situmorang, Rivaldo Sri Rahmadani Fitri Syahrastani, Syahrastani Syamsurizal Syamsurizal - - Syamsurizal Syamsurizal Vauzia, Vauzia Violita Violita Violita Violita Viona, Alda Wilna Sari Windi Nopitasari Yossi Rahmadeni Yulanda Saputri Yuni Ahda Yusrizal Y Yusrizal Y Yusrizal Yusrizal Zulyusri, Zulyusri