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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan Jurnal Ilmu dan Teknologi Kelautan Tropis Buletin Kimia VALENSI CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) JURNAL KIMIA SAINS DAN APLIKASI Microbiology Indonesia Current Biochemistry The Journal of Pure and Applied Chemistry Research Jurnal Riset Kimia Indonesian Journal of Pharmaceutical Science and Technology Jurnal Sumberdaya Hayati SC (Savana Cendana) : Jurnal Pertanian Konservasi Lahan Kering BERITA BIOLOGI BIOMA Indonesian Journal of Chemistry QARDHUL HASAN: MEDIA PENGABDIAN KEPADA MASYARAKAT EKOLOGIA : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Menara Perkebunan Jurnal Ilmu Kefarmasian Indonesia Jurnal Jamu Indonesia Jurnal Agronomi Tanaman Tropika (JUATIKA) Pharma Xplore : Jurnal Sains dan Ilmu Farmasi Indonesian Journal of Applied Research (IJAR) Jurnal Pusat Inovasi Masyarakat Makara Journal of Science Molekul: Jurnal Ilmiah Kimia Biosaintifika: Journal of Biology & Biology Education Indonesian Journal of Jamu Biodiversitas Journal of Biological Diversity
LAKSMI AMBARSARI
Department Of Biochemistry, Faculty Of Mathematics And Natural Sciences, Institut Pertanian Bogor. Jl. Meranti, Kampus IPB Dramaga, Bogor 16680, West Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health Social Sciences Other

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INHIBISI ENZIM α-GLUKOSIDASE OLEH SENYAWA FLAVONOID DAUN KELOR (Moringa oleifera) IN SILICO DAN IN VITRO Puspita, Puspa Julistia; Alimah, Shobiroh Nuur; Ambarsari, Laksmi; Wahyuni, Riksa Nur
Current Biochemistry Vol. 10 No. 2 (2023)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.10.2.3

Abstract

Moringa leaves flavonoid have potential to inhibit α-glucosidase but they have low bioavailability, so they are made in nanoparticles. It is also not known which specific flavonoid compounds from Moringa leaves have the potential to inhibit α-glucosidase. This research aimed to determine the inhibition potential of α-glucosidase by the moringa leaves flavonoid in silico through molecular docking and determine the inhibitory activity of α-glucosidase by moringa leaves flavonoid in extracts and nanoparticles in vitro. Moringa leaves flavonoid have potential to be a competitive inhibiton of α-glucosidase with the highest to lowest inhibitory potential are cryptochlorogenic acid, quercetin-3-O-beta-D-glucopyranoside, quercetin-3-glucoside, kaempferol-3-O-a-ramnoside, kaempferol-3-O-glucoside, epicathechins, catechins, quercetin, kaempferol, glucomoringin isothiocyanate. Cryptochlorogenic acid has the best potential with ΔG and Ki values -8.5 kcal/mol and 0.5788 μM. Inhibition α-glucosidase moringa leaves flavonoid in extract and nanoparticles respectively are classified as inactive (IC 50 = 5.84x10 3 ppm) and active (IC 50 = 1.59x10 1 ppm) in vitro, so nanoparticles can increase the inhibitory activity.
Optimization of PCR Conditions for Adding XhoI Restriction Sites to the Glucose Oxidase Gene of Aspergillus niger IPBCC 08.610 Aryani, Hanifah; Akbar, Nadhira Fathiaz; Kurniatin, Popi Asri; Fuad, Asrul Muhamad; Ambarsari, Laksmi
Current Biochemistry Vol. 11 No. 1 (2024)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.11.1.2

Abstract

Glucose oxidase (GOX) is naturally produced by fungi Aspergillus niger. The GOX enzyme catalyzes the oxidation reaction of β-D-glucose to produce δ-gluconolactone and hydrogen peroxide a (H2O2). Hydrolysis of δ-gluconolactone will produce gluconic acid. Gluconic acid and its derivatives have benefits in the pharmaceutical field as a drug for mineral deficiencies. A. niger IPBCC 08.610 is a local isolate that produce intracellular GOX with higher yield than extracellularly. GOX can be expressed extracellularly by cloning into the expression vector pPICZαB which has the signal peptide α-mating factor (α-MF). GOX gene construction needs to be done by adding some features such as XhoI restriction sites at the 5' and 3' ends, XbaI restriction site at 3’ side, and spacer peptide glu-ala-glu-ala at 5’ side. This research aims to optimize Polymerase Chain Reaction (PCR) conditions in two stages of amplification, stage I to copy the GOX gene and stage II to add those features so it is hoped that recombinant GOX can increase gluconic acid production. The results of primer concentration optimization showed that primers with a concentration of 10 µM produced clearer PCR amplicons than those with a concentration of 20 µM. The optimal temperature for amplification stage I is 58°C. The amplification stage II annealing temperature was modified with the first ten cycles based on the lowest Tm of primer value, 52°C, and the subsequent 25 cycles based on the highest Tm of primer value, 61°C.
Evaluasi Faktor Yang Mempengaruhi Ekstraksi Rimpang Temu Ireng Berdasarkan Aktivitas Penghambatan α-Glukosidase Artika, I Made; Ambarsari, Laksmi; Nurcholis, Waras
Jurnal Jamu Indonesia Vol. 3 No. 2 (2018): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v3i2.57

Abstract

Terdapat beberapa faktor yang dapat digunakan dalam ekstraksi rimpang temu ireng yang memiliki aktivitas farmakologi. Penelitian ini bertujuan untuk mengidentifikasi faktor pH, konsentrasi etanol, suhu, waktu, dan rasio cairan-padatan dalam proses ekstraksi rimpang yang memberikan kontribusi signifikan sebagai inhibitor enzim a-glukosidase. Rancangan optimasi dilakukan dengan menggunakan faktoral fraksional 2(5-1). Urutan hasil dari ekstrak yang paling berkontribusi sebagai inhibitor enzim a-glukosidase: konsentrasi etanol > rasio cairan terhadap padatan > suhu > waktu > pH. Diantara faktor yang dianalisis menunjukkan bahwa konsentrasi etanol dan interaksi antara pH dan konsentrasi etanol merupakan faktor yang signifikan sebagai inhibitor enzim a-glukosidase. Dengan demikian, rimpang temu ireng memiliki potensi sebagai anti-hiperglikemia alami.
Gelatin Extraction and Characterization from Femur Bones of Bovine and Porcine with Acid Process Purwantiningsih Sugita; Muhamad Rifai; Ambarsari, Laksmi; Rahayu, Dyah Utami Cahyaning; Dianhar, Hanhan
Jurnal Jamu Indonesia Vol. 6 No. 1 (2021): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v6i1.188

Abstract

Gelatin has been widely used as an additive in food industry pharmaceutical, and cosmetic. The similar physical appearance between bovine and porcine gelatin causes an issue for some communities like a Muslim due to awareness of halal food. This study aims to produce gelatin from femur bones of bovines with acid hydrolysis and their characteristics compared to standard gelatin of bovine and porcine. Bovine and porcine bones were soaked in 5% HCl for 10 days and every 2 days a HCl solution was replaced to get ossein. Ossein is hydrolyzed by gradual heating at 65, 75, and 85oC. Gelatin confirmed by the physico-chemical characters, FT-IR and analysis amino acid with HPLC.The results showed that the yield of bovine gelatin was 4.33%. The physico-chemical characters of bovine gelatin resulting from isolation and bovine gelatin standards are in conformity with porcine gelatin standards and meet the requirements of SNI 06-3735-1995 and GMIA. Therefore, bovine gelatin is specifically capable of substituting porcine gelatin for application in the pharmaceutical field. The FTIR spectrum of bovine gelatin shows the presence of amide A, amide I, amide II and amide III groups. The amino acid characters of gelatin were identified as glycine (13.57%) and proline (1.62%) for bovine gelatin and glycine (0.51%) and proline (0.09%) for porcine gelatin.
Novel Compounds Design of Acertannin, Hamamelitannin, and Petunidin-3-Glucoside Typical Compounds of African Leaves (Vernonia amygdalina Del) as Antibacterial Based on QSAR and Molecular Docking Kurniawan, Ilham; Ambarsari, Laksmi; Kurniatin, Popi Asri; Wahyudi, Setyanto Tri
Jurnal Jamu Indonesia Vol. 8 No. 2 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i2.326

Abstract

Antibacterial secondary metabolites such as tannins and their derivatives are found in the Vernonia amygdalina Del. Antibiotic resistance can develop due to overuse, reducing the efficacy of drugs to prevent and treat infections. This research aims to use the Quantitative Structure-Activity Relationship (QSAR) and the semi-empirical method Austin Model 1 (AM1) to design a modified novel compound from African leaves that has improved antibacterial activity. This research includes a descriptor calculation of QSAR using AM1 MOE on typical compounds from African leaves, and calculation results are chosen based on a multilinear regression statistical analysis. The model equation represents the three primary parameters of QSAR, which are electronic, hydrophobic, and steric parameters, which will be used to measure modified compounds. Molecular docking using Autodock Tools (The Scripps Research Institute, USA), and analysis of results of docking Autodock Tools using Discovery Studio 3.5 Client. The best QSAR model obtained is LogEC50 = (0.829 x LogP) - (1,302 x AM1_HOMO) - (0.339 x AM1_dipole) - (5,128 x mr) + (0.145 x vol) - (11,355). The results showed that EC50 prediction of modified hamamelitannin has the best activity with the lowest ΔGbind -9.0 kcal/mol and inhibition constant of 0.249 μM. In summary, the novel compound's design calculation has better antibacterial activity, as indicated by a lower EC50, than fosfomycin or compounds without modification. The modified hamamelitannin compound was found to have better antibacterial activity (prediction EC50 = 0.1933 μM) than the original (experimental EC50 = 145.50 μM).
Molecular Docking Study of Bioactive Compounds from Curcuma aeruginosa Roxb. as Antioxidant and Anticancer Activities Ambarsari, Laksmi; Nurjanah, Siti; Artika, I Made; Fatriani, Rizka
Jurnal Jamu Indonesia Vol. 8 No. 2 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i2.327

Abstract

Cancer is one type of dangerous disease that is increasing every year. Free radicals are the cause of gene mutations (cancer). Cancer will develop uncontrollably due to the occurrence of the cell cycle and the presence of compounds that trigger cell proliferation and inhibit the process of apoptosis. This cancer treatment is carried out by giving cyclin-dependent kinase2 (CDK2) and cyclooxygenase-2 (COX-2) inhibitor drugs to inhibit cancer development, as well as lipoxygenase (LOX) inhibitor drugs for the formation of free radicals. Curcuma aeruginosa RoxB., the plant, is known to have the potential for antioxidant and anticancer properties. This study aims to determine the molecular interaction between the dominant compound in the ethanol extract of C. aeruginosa with CDK2, COX-2, and LOX receptors. The type of ligand interaction with the receptor was determined through the parameters of affinity energy (∆G), inhibition constant (Ki), type of interaction, and percentage of binding site similarity (%BSS). The results showed that the gajutsulacton A had the best potential in inhibiting CDK2. The cucumenol may be a COX-2 inhibitor, and there are no compounds that can inhibit LOX as well as an antioxidant. Thus, our findings demonstrate the potential for C. aeruginosa bioactive to serve as anticancer candidate molecules against CDK2 and COX-2 receptors.
Cloning of the GOX-Xho Gene IPBCC 08.610 into Plasmid pTA2 and Its Characterization Aryani, Hanifah; Akbar, Nadhira Fathiaz; Fuad, Asrul Muhamad; Ambarsari, Laksmi; Kurniatin, Popi Asri
Jurnal Kimia Valensi Jurnal Kimia VALENSI, Volume 10, No. 2, November 2024
Publisher : Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v10i2.39602

Abstract

Glucose oxidase (GOX) from Aspergillus niger catalyzes the oxidation of β-D-glucose to δ-gluconolactone and hydrogen peroxide, making it valuable for industrial applications. Intracellular GOX exhibits higher activity than its extracellular counterpart. This study focuses on enhancing the extracellular production of GOX through recombinant DNA technology. This study aimed to reconstruct the GOX gene by adding XhoI sites at both ends, inserting a glu-ala-glu-ala spacer at the 5' end, and introducing an XbaI site at the 3' end. These modifications facilitate the cloning of the GOX-Xho gene into the pTA2 vector and its subsequent ligation into the pPICZαB expression vector, allowing for extracellular production of GOX through fusion with the α-mating factor (α-MF) signal peptide. The GOX-Xho gene was successfully amplified, cloned, and characterized. The pTA2-GOX-Xho recombinant plasmid was verified through sequencing and restriction analysis, confirming the present and correct orientation of the 1797 bp GOX-Xho gene. However, sequencing revealed several point mutations, necessitating further computational analysis to predict their impact on the enzyme's structure and function before recombinant protein expression.
Phytochemicals and Lipase Inhibition of Citronella, Galangal, and Sand Galangal: In Vitro–In Silico Approaches Aghnia, Diya; Hasim, Hasim; Ambarsari, Laksmi; Faridah, Didah Nur; Slameut, Fitria; Kandi, Rizky Putra
Jurnal Teknologi dan Industri Pangan Vol. 36 No. 2 (2025): Jurnal Teknologi dan Industri Pangan
Publisher : Perhimpunan Ahli Teknologi Pangan Indonesia bekerjasama dengan Departemen Ilmu dan Teknologi Pangan, IPB University Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/jtip.2025.36.2.264

Abstract

Obesity is a major global health concern, often treated by inhibiting pancreatic lipase to reduce fat absorption. While chemical-based medicine is a widely used synthetic inhibitor, its side effects highlight the need for safer, natural alternatives. This study aimed to characterize the total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity, and pancreatic lipase inhibition of citronella leaf (Cymbopogon nardus), galangal rhizome (Alpinia galanga), and sand galangal rhizome (Kaempferia galanga) through in vitro and in silico analyses and to identify the potential phytochemical compounds responsible for the activity. Citronella showed the highest TPC, TFC, and FRAP values (14.20±0.21 mg GAE/g, 17.36±9.51 mg QE/g, and 92.01±1.88 µmol TE/g, respectively), indicating strong antioxidant potential. Galangal exhibited the highest extraction yield (21.86±5.34%) and DPPH activity (1.09±0.27 µmol TE/g). In vitro lipase inhibition assays revealed galangal and sand galangal had moderate inhibitory effects (IC50= 401.2±18.24 and 374±11.24 µg/mL), while citronella showed weak activity. LC-MS/MS analysis of galangal identified eight compounds, including galangin, eugenol, and galanganol C. Molecular docking showed galangin had the strongest binding affinity (ΔG= -10.239 kcal/mol), interacting with catalytic residues Ser152 and His263 of pancreatic lipase via hydrophobic and electrostatic interactions. These findings suggest that citronella, galangal, and sand galangal possess potential as natural pancreatic lipase inhibitors, with galangal particularly galangin showing the most promising activity for obesity prevention and management.  
Optimization of Extraction Conditions to Enhance Polyphenol Content and Antioxidant Activity İn Orthosiphon Aristatus Leaves Febriyanto, Dimas; Setyawati, Inda; Ambarsari, Laksmi; Nurcholis, Waras
Molekul Vol 21 No 1 (2026)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2026.21.1.16820

Abstract

ABSTRACT. Orthosiphon aristatus (Java tea) is a medicinal plant known for its high polyphenol content and antioxidant properties. Unlike previous studies that focused solely on solvent type or temperature, this study simultaneously optimizes temperature and pH extraction parameters to achieve maximal bioactive compound recovery. This study aimed to optimize extraction parameters—temperature and pH—to enhance polyphenol yield and antioxidant activity in methanolic extracts of Java tea leaves. Samples were extracted at temperatures ranging from 30 °C to 80 °C (pH 7) and pH levels from 2 to 6 (at room temperature). Total phenolic content (TPC) and total flavonoid content (TFC) were determined to quantify polyphenol levels, and antioxidant activity was evaluated using four complementary in vitro assays: DPPH, ABTS, FRAP, and CUPRAC. The highest TPC (9.68 mg GAE/g DW) and TFC (6.53 mg QE/g DW) were observed at 70 °C and 80 °C, respectively. Maximum antioxidant activities were observed at 70–80 °C and pH 2–3, with peak values of 102.71 µmol TE/g DW (ABTS), 101.9 µmol TE/g DW (FRAP), and 56.12 µmol TE/g DW (CUPRAC). A strong correlation was found between phenolic content and antioxidant capacity. These findings highlight the critical role of extraction conditions in maximizing the biofunctional potential of O. aristatus and support its application in pharmaceutical and nutraceutical formulations. The study provides a scientific basis for developing standardized extraction protocols to improve functional ingredient consistency in herbal product development. Keywords: Antioxidant activity, extraction optimization, Orthosiphon aristatus, pH effect, temperature
Demonstrasi Pengolahan Bubuk Jahe Merah sebagai Ide Wirausaha Minuman Instan Berkhasiat di Desa Batu Busuk Asvarhoza, Genta; Halim, Muhammad; Sarmila, Sarmila; Putra, Adriansyah Nanda; Annisa , Alifia Mutiara; Winarti, Novia; Hamidah, Wanda; Vino , Okta; Yudha, Trah; Elvaza, Azzara Putri; Ambarsari, Laksmi
Jurnal Pusat Inovasi Masyarakat Vol. 5 No. 1 (2023): April 2023
Publisher : Direktorat Pengembangan Masyarakat Agromaritim, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpim.5.1.68-76

Abstract

Umumnya jahe digunakan oleh masyarakat di Desa Batu Busuk untuk berbagai kepentingan, misalnya sebagai campuran bahan makanan dan minuman. Jahe merah memiliki banyak manfaat yang berguna untuk kesehatan. Selama masa pandemi ini kebutuhan masyarakat akan jahe mengalami peningkatan sehingga perlu adanya inovasi produk olahan jahe salah satunya bubuk jahe merah instan. Pengolahan jahe merah yang dipadukan dengan gula serta bisa ditambahkan rempah lain sebagai pelengkap sehingga menjadi bubuk jahe merah yang dinamakan jahe next level (JNL). Olahan jahe merah ini nantinya bisa dijadikan minuman yang berkhasiat untuk kesehatan. Pengolahan jahe merah menjadi bubuk jahe merah meningkatkan nilai mutu produk pangan tersebut di samping khasiatnya yang sangat bermanfaat. Bubuk jahe merah dapat menjadi produk pangan alternatif bagi masyarakat yang ingin mengonsumsi minuman jahe dengan penyajian yang mudah yaitu dengan cara diseduh dengan air hangat. Selain itu, pengolahan bubuk jahe merah juga dapat menjadi ladang wirausaha yang menguntungkan bagi masyarakat di Desa Batu Busuk. Melalui demonstrasi pengolahan bubuk jahe merah diharapkan dapat memberikan ide produk wirausaha minuman berkhasiat bagi masyarakat di Desa Batu Busuk.
Co-Authors -, Suryani . SURYANI A.E. Zainal Hasan Ade Heri Mulyati Adrian Adiva Aghnia, Diya Agnia Nurul Jannati Agung Isnanto Agustina L.N. Aminin Ahmad Irvan Pratama Ainia Hanifitri Aisyah Girindra Akbar, Nadhira Fathiaz Akhiruddin Maddu Akhmad Endang Zainal Hasan Alimah, Shobiroh Nuur Andiany, Fairuz Eka Anggita Aziz Pratiwi ANINDA INDRIANI Anja Meryandini Annisa , Alifia Mutiara Annisa Dhiya Athiyyah Khanza Aris Tri Wahyudi Aris Tri Wahyudi Arwansyah Arwansyah Aryani, Hanifah ASMAUL CHUSNA AL ANSHORY Asrul Muhamad Fuad, Asrul Muhamad Assifah Eryandini Assifah Eryandini Asvarhoza, Genta Ayu Lestari Azhari, Muhammad Alwin Chelsea Chelsea, Chelsea Chelsy Narita Choirunnisa Miftahul Jannah Deki Geraldi Dewi Seswita Zilda Diana Widiastuti DIMAS ANDRIANTO Dimas Andrianto Djarot Sasongko Hami Seno DWI NINGSIH SUSILOWATI DWI NINGSIH SUSILOWATI Dwi SUBIYARTI Dwiningsih Susilowati Edy Djauhari Purwakusumah Edy Djauhari Purwakusumah Efi Sanfitri Harahap Elvaza, Azzara Putri Faliha Arinda Lestari Farhan Azhwin Maulana Fathin, Muhammad Faris Fatriani, Rizka Febrianti, Riska Febriyanto, Dimas G Jeni christi A GIA PERMASKU Giovanny, Lisa Gustini Syahbirin Gustini Syahbirin H. M. Mochtar Hamidah, Wanda Hanaa Ashiilah Hanhan Dianhar Harahap, Efi Sanfitri HARTUTIK EKA SUSANTI Haryo Tejo Prakoso Hasim - Hasim Hasim Hayati Minarsih Heri Purwoto Hidayat, Radika Evita Husnawati Husnawati Husnawati, . I MADE ARTIKA I Made Samudera I Made Samudera, I Made I MADE SAMUDRA Ifa Manzila Ifa Manzila Ika Yuni Astutik Ika Yuni Astutik, Ika Yuni Ike Wahyuni Putri Ilham Kurniawan Ilham Kurniawan Imelia Dewi Imron RIYADI Inayah, Mazidah Noer Inda Setyawati Inda Setyawati, Inda Irma Rahmayani IRMANIDA BATUBARA Isnanto, Agung Jaya Hardi Kandi, Rizky Putra Kapitan, Origenes Boy Karichsa Hariana Latifah K Darusman Lestari, Faliha Arinda Liliyani , Ni Putu Peggy Lisa Giovanny Made Suhandana Madyastuti, Rini Maheswari Alfira Dwicesaria Marfira, Nurul MARLIN MARLIN Martini Hudayanti Mega Safithri Mega Safithri Miantika, Shafillah Muhamad Rifai Muhamad Rifai Muhammad Alwin Azhari Muhammad Farhan Muhammad Faris Fathin Muhammad Halim, Muhammad Mustika Luthfia Mustika Permatasari Mustopa, Syahrul Nabila Nur Nafiati Nadine Rudiyana Natalia Natalia Marbun Nisa Mubarik Nisa Rachmania Mubarik NISA RACHMANIA MUBARIK NOVIAN LIWANDA Nur Qadri Rasyid Nurhidayat Nurhidayat Nurma Angeliani Komalasari Nurul Marfira Nuur’Alimah, Shobiroh Popi Asri Kurniatin Pratama, Ahmad Irvan Purwantiningsih Sugita Purwanto, Ukhradiya Magharaniq Safira Purwatiningsih Sugita Puspa Julistia Puspita Putra, Adriansyah Nanda Putri, Ike Wahyuni Radika Evita Hidayah Rahayu, Dyah Utami Cahyaning Rahmadhani Ambarsavitri RAISADIBA PUTRI BOVANI Rara Annisaur Rosyidah Rasyid, Nur Qadri Ridho Pratama Rifany Fairuz Aqilah Riki Riki Riksa Nur Wahyuni Rini Kurniasih Rini Kurniasih, Rini Rini Novita Riska Febrianti Rury Eryna Putri Rury Eryna Putri Sakina Maya Maulawi Sanro Tachibana Sarmila, Sarmila Setyanto Tri Wahyudi Shobiroh Nuur' Alimah Shobiroh Nuur'Alimah Shobiroh Nuur'Alimah Siswanto Siswanto Siti Nur'aeni Siti Nurjanah Siti Warnasih Slameut, Fitria Soekarno Mismana Putra Sri Wahyuni Steffanus Gozales Suryani - SURYANI SURYANI Suryani Suryani Syabilla Aulia Rahman Syamsul Falah Syamsul Falah TATI NURHAYATI Tirta Setiawan Tony Sumaryada Tri Puji Priyatno Tri Puji Priyatno Uswatun Hasanah Vino , Okta Wahyuni, Riksa Nur WARAS NURCHOLIS Waras Nurcholis Waras Nurcholis Winarti, Novia Yadi Suryadi Yadi Suryadi Yudha, Trah Zahra Silmi Muscifa Zuniar Subastian