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All Journal Zuriat Kolaboratif Bioscience Bioeducation Journal Jurnal Biologi dan Pembelajarannya (JBP) SEMIRATA 2015 Jurnal Pendidikan Biologi Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis JURNAL PENDIDIKAN TAMBUSAI Acta Biochimica Indonesiana Global Conference Index AL-ULUM: JURNAL SAINS DAN TEKNOLOGI Atrium Pendidikan Biologi Serambi Biologi Pelita Eksakta Abdi: Jurnal Pengabdian dan Pemberdayaan Masyarakat Jurnal Ilmu Pertanian Tirtayasa Sainstek : Jurnal Sains dan Teknologi Jurnal Biogenerasi BIO-EDU: Jurnal Pendidikan Biologi Konservasi Hayati Suluah Bendang: Jurnal Ilmiah Pengabdian Kepada Masyarakat Science and Environmental Journals for Postgraduate BioEksakta : Jurnal Ilmiah Biologi Unsoed Journal of Educational Sciences JNPM (Jurnal Nasional Pendidikan Matematika) Ta'dib Jurnal Serambi Biologi Biodik: Jurnal Ilmiah Pendidikan Biologi Literasi Bahasa dan Sastra Jurnal

Azwir Anhar

Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Negeri Padang

Author-ID : 228996

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Articles

Title

The effect of Trichoderma spp. against germination speed of batok glutinous rice seeds (Oryza sativa L. var. glutinosa) Wibi m Syofian; Azwir Anhar
Bioscience Vol 6, No 1 (2022): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202261109181-0-00

Batok glutinous rice especially local varieties, is one of the agricultural commodities that can be used as a source of food. Besides having a good taste, batok glutinous rice is also not easily attacked by pests and rice seeds easily obtained. However, obstacles in the utilization of glutinous rice for local varieties are long harvest time and low production. Therefore, seed priming is carried out by utilizing biofertilizer derived from the rhizosphere fungus, Trichoderma spp. Trichoderma is a function that is classified as Plant Growth Promoting Function (PGPF) which is able to stimulate plant growth by producing growth regulators. This study aims to determine the response of the germination of batok glutinous rice seeds against of Trichoderma spp.This research is an experimental study using a completely randomized design with 7 treatments and 3 replications. The treatment given was soaking seeds with 6 types of Trichoderma spp. isolates and 1 control soaked with aquadest. The data obtained were analyzed statistically using ANOVA. If there are significant differences, further tests of DMRT are carried out with α = 5%.The results obtained indicate that Trichoderma spp. no significant effect on the percentage of germination and vigor index, but significant effect on the germination speed with RE isolates is the best isolate in increasing the seed germination speed.Beras ketan Batok khususnya varietas lokal, merupakan salah satu komoditas pertanian yang dapat dimanfaatkan sebagai sumber pangan. Selain memiliki rasa yang enak, beras ketan batok juga tidak mudah terserang hama dan bibit padi mudah didapatkan. Namun kendala dalam pemanfaatan beras ketan untuk varietas lokal adalah waktu panen yang lama dan produksi yang rendah. Oleh karena itu, priming benih dilakukan dengan memanfaatkan pupuk hayati yang berasal dari cendawan rizosfer Trichoderma spp. Trichoderma merupakan fungsi yang tergolong Plant Growth Promoting Function (PGPF) yang mampu merangsang pertumbuhan tanaman dengan memproduksi zat pengatur tumbuh. Penelitian ini bertujuan untuk mengetahui respon perkecambahan benih ketan batok terhadap Trichoderma spp. Penelitian ini merupakan penelitian eksperimen dengan menggunakan rancangan acak lengkap dengan 7 perlakuan dan 3 ulangan. Perlakuan yang diberikan adalah perendaman benih dengan 6 jenis Trichoderma spp. isolat dan 1 kontrol direndam dengan aquadest. Data yang diperoleh dianalisis secara statistik menggunakan ANOVA. Jika terdapat perbedaan yang signifikan, selanjutnya dilakukan uji DMRT dengan = 5%. Hasil yang diperoleh menunjukkan bahwa Trichoderma spp. tidak berpengaruh nyata terhadap persentase daya berkecambah dan indeks vigor, namun berpengaruh nyata terhadap kecepatan berkecambah dengan isolat RE merupakan isolat terbaik dalam meningkatkan kecepatan berkecambah benih.

Respon Pertumbuhan Benih Padi Varietas Anak Daro Asal Solok Terhadap Isolat Trichoderma Indeginous Azwir Anhar; Dwi HIlda Putri; Febri Doni; Linda Advinda
Bioscience Vol 4, No 1 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Trichoderma spp adalah jamur non patogen yang berasosiasi dengan rhizosfer dan daun tanaman. Jamur ini juga mampu mengendalikan penyakit dan menghasilkan zat pengatur tumbuh, sehingga potensial digunakan dalam pertaniankhususnya dalam budidaya pertanian organik. Penelitian kemampuan Trichoderma spp dalam meningkatkan pertumbuhan benih padi masih terbatas. Penelitian ini, melaporkan kemampuan Trichoderma setempat untuk meningkatkan poertumbuhan kecambah benih padi. Hasil penelitian menunjukkan bahwa isolat Trichoderma mampu meningkatkan daya kecambah dan vigor benih padi. Hasil penelitian membuktikan bahwa isolat Trichoderma indegenous memiliki potensi untuk digunakan sebagai pengatur pertumbuhan benih padi. Hasil penelitian meningkatkan pemahaman lebih lanjut tentang peranan jamur dalam meningkatkan pertumbuhan padi

The Effect of Mango Juice (Mangifera Indica) and Long Ripening on the Quality Of Cow's Milk Curd Selly Fildawati; Linda Advinda; Azwir Anhar
Bioscience Vol 1, No 2 (2017): Biology
Publisher : UNIVERSITAS NEGERI PADANG

The curd is a traditional food Minangkabau in West Sumatra. This product is made from buffalo milk made with buffalo milk entering into the bamboo and covered with banana leaves and left at room temperature for about 1 to 2 days. Due to the limited availability of buffalo milk, buffalo milk is replaced with cow's milk. Obstacles encountered in the development of the curd is curd less flavorful savory and original taste sour curd so that the public interest is reduced. Therefore, we need a way to neutralize the smell and taste of sour curds, one of which is the juice of mango (Mangifera indica). The purpose of this study was to determine the effect of mango juice and long ripening on the quality of cow's milk curd. This study is experimental and descriptive. The design used was a 3x2 factorial randomized block design with three replications. A factor is the mango juice consisting of 3 treatment, and long ripening factor B is composed of 2 treatments. Research was conducted in January 2016 in the Laboratory of Livestock Product Technology Faculty of Animal UNAND. Parameters measured were total acid content, protein content, and organoleptic. Data were analyzed by descriptive qualitative analysis of variance (ANOVA) and a further test DNMRT at 5% level. The results of this study are the addition of mango juice and long curing does not affect the total acid content (% TAT). However, the addition of mango juice and long curing effect on protein levels although there is no interaction between the concentration and duration of ripening. Lowest total acid level was the control treatment on a long curing 48 hours and total acid concentration is the highest concentration of 3% at 36 hours. Lowest protein content that is the control treatment at 36 hours and the highest concentration of 3% at 48 hours. The results of organoleptic tests show the variation of each treatment. Keyword: cow's milk curd, mango juice, total acid content, protein content, organoleptic.

Comparison of Three Different DNA Isolation Methods To Degradate The Trichoderma Fungi Cell Wall Widya Ruchi; Dwi Hilda Putri; Azwir Anhar
Bioscience Vol 3, No 1 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Dinding sel jamur Trichoderma tersusun atas senyawa kitin yang sangat kokoh dan resisten terhadap aktivitas enzim. Teknik dan metode spesifik diperlukan untuk mendegradasi dinding sel jamur. Penelitian ini bertujuan untuk menganalisis metode isolasi DNA terbaik dalam melisiskan dinding sel jamur dengan membandingkan beberapa metode isolasi. Metode isolasi yang digunakan adalah DNeasy Plant Mini Kit, kombinasi DNeasy Plant Mini Kit dan pemanasan, serta kombinasi DNeasy Plant Mini Kit dan cara fisik (menggerus sampel dengan menggunakan mortar dan pestle dalam nitrogen cair). Penelitian ini dilakukan pada bulan Maret sampai September 2018 yang bertempat di Laboratorium Mikrobiologi, Laboratorium Bioteknologi dan Genetika, serta Laboratorium Penelitian Terpadu, FMIPA, UNP. Data dianalisis secara kualitatif dengan elektroforesis gel agarose dan secara kuantitatif dengan menghitung nilai kemurnian dan konsentrasi DNA menggunakan Nanodrop. Hasil penelitian menunjukkan bahwa metode kombinasi DNeasy Plant Mini Kit dengan cara fisik memberikan hasil isolasi DNA dengan konsentrasi yang tertinggi yaitu 2,4 μg/ml dengan nilai kemurnian 1,857. Pada metode DNeasy Plant Mini Kit dan metode kombinasi DNeasy Plant Mini Kit dengan pemanasan, konsentrasi DNA yang diperoleh berturut-turut 1,211 µg/ml dan 0,933 µg/ml dengan nilai kemurnian 1,728 dan 1,708. Hasil uji elektroforesis menunjukkan adanya pita DNA berupa garis tipis pada sampel yang diisolasi dengan kombinasi metode kit dan cara fisik, sedangkan pada kedua sampel lainnya tidak ditemukan adanya pita DNA. Dapat disimpulkan metode isolasi DNA yang dapat dijadikan rujukan untuk mendegradasi dinding sel jamur Trichoderma adalah metode kombinasi kit dengan nitrogen cair dengan catatan dibutuhkan penambahan kadar isolat yang digunakan.Kata Kunci: Isolasi DNA, Dinding sel, Trichoderma

The Potential of Hot Water Sapan Sungai Aro Thermophilic Bacteria Consortium in Producing Bioethanol Inayatul Fatia; Irdawati Irdawati; Linda Advinda; Azwir Anhar
Bioscience Vol 7, No 2 (2023): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v7i2.123265

Biofuel is a potentially renewable alternative fuel in Indonesia. Bioethanol is one example of the most commonly used biofuel. Microorganisms of thermophilic bacteria are known to contribute to the production of bioethanol. Thermophilic bacteria are efficient against high temperature conditions so as to minimize contamination. Production of bioethanol can also use joint culture (consortium). Bioethanol production using a microbial biculture consortium is known to significantly increase the level of bioethanol production. The purpose of this study was to determine the compatibility and to determine the optimum potential of the thermophilic bacterial biculture consortium of Sapan Sungai Aro hot water for bioethanol production. This research is a type of descriptive research. To test the cooperation between consortium isolates of thermophilic bacteria producing bioethanol, a compatibility test was carried out using the disk diffusion method. Then the consortium isolates were fermented in liquid TMM (Thermophilic Minimum Media) medium, the bioethanol content was measured after distillation using a pycnometer. The results of the bacterial compatibility test showed that there was one pair of isolates that were not compatible, namely SSA 8 & SSA 14 due to the presence of a clear zone. On research results. The production of bioethanol by a consortium of thermophilic bacteria gives more optimal results compared to a single isolate. The best thermophilic bacterial biculture consortium from the Sapan Sungai Aro hot spring in producing biofuels is SSA 14 & SSA 16 which is 3.009%.

PRODUCTION OF SPESIFIC XYLANASE ENZYME BY MUDIAK SAPAN HOT WATER THERMOPHILIC BACTERY Irdawati Irdawati; Muhammad Deedat Ayasy; Azwir Anhar; Linda Advinda; Yusrizal Yusrizal
Bioscience Vol 8, No 1 (2024): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v8i1.122728

Xylanase is an extracellular enzyme capable of hydrolyzing hemicellulose so that it can convert xylan into xylose. Thermostable xylanase enzymes can be produced by thermophilic bacteria. Thermophilic bacteria are used because they have advantages such as the ability to increase enzyme production in adjustable catalytic specifications. Bacteria are not only in a single form but also exist in a mixed form called a consortium. Compared with a single isolate, the performance of the consortium is better. Consortium is a mixture of microbial populations in the form of communities that have cooperative, commensal, and mutualistic relationships. This study aims to look at the cooperation between the consortium isolates and the consortium's ability to produce xylanase enzymes. This research is a descriptive study. The bacterial consortium is fermented in beechwood xylan medium. The results of this study were that MSS 11, MS 18, MS 16 consortium produced the highest xylanase enzyme activity, namely 12,887.Xilanase merupakan enzim ekstraseuler yang mampu menghidrolisis hemiselulosa sehingga dapat merubah xilan menjadi xilosa. Enzim xilanase termostabil dapat diproduksi oleh bakteri termofilik. Bakteri termofilik digunakan karena dapat memiliki keunggulan seperti aktivitas dan peningkatan spesifikasi katalisis yang dapat diatur. bakteri tidak hanya dalam bentuk tunggal namun juga ada dalam bentuk campuran yang disebut konsorsium. Dibandingkan dengan isolat tunggal, kinerja konsorsium lebih baik. Konsorsium merupakan campuran populasi mikroba dalambentuk komunitas yang mempunyai hubungan kooperatif, komensal, dan mutualitsik. Penelitian ini bertujuan untuk melihat kerjasama antar isolat konsorsium dan kemampuan konsorsium dalam menghasilkan enzim xylanase. Penelitian ini bersifat deskriptif. Pada penelitian ini, konsorsium bakteri difermentasikan dalam medium beechwood xylan. Hasil penelitian ini adalah konsorsium MSS 11, MS 18, MS 16 menghasilkan aktivitas enzim xylanase spesifik tertinggi yaitu 12,887.

Floristic composition and economic value of trees at agroforestry parak in Tanjung Raya District, Agam Regency, West Sumatra Indonesia Alfitrah Habibullah. M; Irma Leilani eka Putri; Azwir Anhar; Reki Kardiman
Bioscience Vol 8, No 1 (2024): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v8i1.125863

This study aimed to investigate the floristic composition and the economic values of trees at the agroforestry parak in Maninjau, Tanjung Raya District. Four different villages were selected purposively according to the tree structure of parak, and each village represented each compass point surrounding the Maninjau Lake. 20 x 100 m plot was established at each sites, where all tree species were identified, and five owner of the parak at each location were asked about the fruiting and harvesting season, management and potential income from each of tree species. 20 tree species belong to 13 families were found across the sites, but only five were occured at all sites, while the others were missed at least from one site. Most species produced fruits with fruiting and harvesting season ranged from one to three times a year, but only seven species knew the income from selling the commodity. From all seven species, which varied on the harvesting season, the owner of each parak gained up to four million IDR per month. This study showed that variation of species and the harvesting season beneficial the owner of the parak and may kept the parak as primary or alternative livelihood.

Co-Authors Abdul Fatah, Firdaus Abdul Razak Abdul Razak Afyeni, Yutrin Nisya Al Adawiyah Alfitrah Habibullah. M Amrianto Amrianto Amrianto Amrianto, Amrianto Andesmora, Evan Vria Annisa Putri Aslim Rasyad Atiqahh Muthiah Beni Gusnadi Celsi Ananda Chahyunisa, Alifia Desmayanti, Resti Dezi Handayani Diana Susanti Djoelvinanda, Habibullah Dwi Hilda Putri Edwin Musdi Eka Vidya Putra Elmanazifa, Sintia F, Farida Fadhlurrohman, Reza Falta, Lira Andika Farhanah Shofwah Verina Farikh, Muhammad Farma, Sisca Alicia Febri Doni Feby Djumaita Sari Fevria, Resti Fitri, Selfi Kurnia Fitri, Yosi Maidia Fitria, Laili Fitriana Fitriana Gustina Indriati Handayani, Delsi Fitri Handayani, Lena Putri Hapni, Nur Haqil Triyatdipa hilda, Dwi hilda putri Huda Asra, Rahmatul Husnul Khatimah I Made Arnawa I Made Arnawa Ilham Rizky Ritonga Inayatul Fatia Indra Yuzandi Indrawani Matondang IRDAWATI Irdawati Irdawati Irdawati Irdawati Irma Lailani Eka Putri Irma Leilani Irma Leilani Eka Putri Irma Leilani Eka Putri Iskandar, Ingriani Kardiman, Reki Kartika Sari Khairunisa Khairunisa Kurniasih, Yulvina Laili, Fitria Leilani Eka Putri Lia Angela Linda Advinda Linda Advinda Listi Handayani Lufri Lufri Mades Fifendy Maiyusri Eka Putri Marten, Threo Wanda Masnidar Masnidar Miftahul Jannah Milka Saputri Monica, Della Trya Moralita Chatri Muhammad Deedat Ayasy Mulyadi, Rival Nadira Nadira Nia Ramadhanti Nuari, Sevira Della Nurfa Dewiza Luzik Oktaviani, Mutia Pebriyeni, Silvi Pertiwi, Lastri Pratama, Chelsylia Dara Puspitasari, Winda Putri Erianti Putri, Cici Adelia Putri, Irma Leilani Eka Putri, Lidya Eka Rahmi Septia Sari, Rahmi Septia Rahmi Zahri Zani Rahmi Zahri Zani Ramadan Sumarmin Ramadhan Sumarmin Reki Kardiman Riska Riska Ristiono Ristiono Ristiono Ristiono Roza Yolanda Safira Nurul Fadila Sagurung, Mariance Dartiani Salma Sakina Sari Wirdaningsih Selaras, Ganda Hijrah Selly Fildawati Sepriadi, Ilham Simbolon, Perima Sintia Elmanazifa Siska Alicia Farma Siti Aisyah Sri Rahmadani Fitri Sunarsan, Debra Fortuna Surya Tati Syafni Nola Putri Syahfitri, Aulia Insyani Syahrastani, Syahrastani Syari, Elsy Melia Tarigan, Siti Nadiah Zahra Br Tazri, Mufidah Insani Tiffany Mantoviana Tomi Apra Santosa Santosa Ulfa, Mariyah Valofi, Nagra Aulia Vauzia Vauzia, Vauzia Violita Violita Violita Violita Violita Wibi m Syofian Widya Ruchi Wilny Chintya Wirdaningsih, Sari Wita Ferwati Wulandari, Tesya Yosefin Nisa Aulia Yuni Ahda Yuni Ahda Yusrizal Y Yusrizal Y Yusrizal Yusrizal Zaenab, Nurul Zulyusri Zulyusri Zulyusri, Zulyusri

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