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The Potency of Olive Oil, Coconut Oil, and Bleached Palm Oil as Clearing Agent in Histological Preparation Ruth Liananda Citra Dolok Saribu; Laili Mufli Zusrina; Murtiadi Erlan Supraitno; Ardaning Nuriliani; Bambang Retnoaji; Hendry T.S.S.G. Saragih; Zuliyati Rohmah
Berkala Ilmiah Biologi Vol 14 No 2 (2023)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v14i2.6528

Abstract

Clearing is an important process in histological preparations. The aim of the clearing process is to remove alcohol from the tissues upon dehydration. The most commonly used clearing agent is xylol, however it is known that there are negative effects from using xylol. This literature study purpose to study the potential of three vegetable oils, i.e., olive oil, coconut oil, and refined palm oil, as clearing agents to replace xylol. This literature study was conducted using search engines such as Semantic Scholar, NCBI, ICAMS, IJPRSE, JOMFP, PubMed, and ResearchGate with the keywords olive oil, coconut oil, palm oil, xylol, clearing agent, and histological preparations. There is no year limitation of references in this literature study. Based on the literatures, it is known that the use of olive oil as a clearing agent produces similar histological preparation qualities to xylol. This can be seen from several criteria that were measured, including the level of tissue translucency and the ability to maintain the quality of the staining. Similar results were shown by purified coconut oil and palm oil, both of which can produce tissues with optimal levels of transparency. The conclusion from this literature study is that refined olive oil, coconut oil, and palm oil can be used as clearing agents for xylol substitutes that are safer for researchers and the environment.
Fixative Solution for Macromolecules in Histological Preparations Hayu Swari Alimi; Ria Vena Septhay Sari; Tia Apriliyani; Ardaning Nuriliani; Bambang Retnoaji; Hendry Saragih; Zuliyati Rohmah
Berkala Ilmiah Biologi Vol 14 No 3 (2023)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v14i3.6531

Abstract

The fixation process is subjected to preserve cells, tissues, and their components so that the structure can be maintained as their alive condition and prevent autolysis. This literature review purpose to determine the appropriate use of fixative solutions for several macromolecules, such as proteins, lipids, carbohydrates, and nucleic acids. The literature review data is obtained form the secondary data included books, journals, or articles which discussed about fixative solutions without a limitation on the year of publication. The databases were sourced from Google Scholar, PubMed, NCBI, Science Direct, SpringerLink, Research Gate, and Nature. The keywords for the book search were tissue processing, pathology, and histology. Keywords for searching journals or articles were protein fixation, formaldehyde fixation lipids, fixatives for nucleic acids, Glyo-Fixx fixatives, mercury chloride fixative lipids, formalin-fixed carbohydrates, and ethanol or methanol for fixative lipids. Based on the literature review conducted, it can be concluded that the use of fixative solutions is according to the macromolecules to be observed.
The Bambara Groundnut’s Potential for Heart Histological Repair in Protein-Deficient Female Mice Puteri, Nabila Ramiza; Gunanegara, Rimonta Febby; Sunarti; Nuriliani, Ardaning
Acta VETERINARIA Indonesiana Vol. 11 No. 3 (2023): November 2023
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.11.3.234-243

Abstract

Defisiensi protein diketahui memberikan perubahan histologis yang signifikan pada jantung seperti hipertrofi, penebalan dinding ventrikel kiri, peningkatan deposisi matriks ekstraseluler dan diameter kardiomiosit, serta fibrosis interstisial. Kacang bambara (Vigna subterranea) dengan kandungan protein dan asam amino berpotensi untuk menanggulangi defisiensi protein dan memperbaiki perubahan struktur histologis pada jantung. Penelitian ini bertujuan untuk mempelajari pengaruh defisiensi protein 10% dan penambahan kacang bambara terhadap struktur histologis jantung mencit betina galur Swiss-Webster. Lima belas jantung dari 5 kelompok dikumpulkan untuk dipreparasi dengan metode parafin setebal 6 μm, dan diwarnai dengan Hematoxylin-Eosin dan Mallory Acid Fuchsin. Data dalam penelitian ini adalah parameter biometrik, lebar kardiomiosit, dimensi internal ventrikel kiri (LVID), ketebalan dinding posterior ventrikel kiri (LVPW), dan struktur histologis otot jantung. Data kuantitatif dianalisis dengan ANOVA satu arah dan post hoc dengan Duncan (p-value=0,05), sedangkan data kualitatif dianalisis menggunakan Kruskal Wallis (p<0,05). Tidak terdapat perbedaan yang signifikan (p>0,05) antara kelompok kontrol dan perlakuan pada parameter biometrik, LVID, LVPW, dan lebar kardiomiosit. Pada parameter struktur histologis otot jantung, kelompok defisiensi protein menunjukkan perubahan berupa lesi atrofi, hipertrofi, nekrosis, dan fibrosis yang lebih banyak dibandingkan dengan kelompok kacang bambara. Dengan demikian, penambahan kacang bambara berpotensi sebagai suplemen protein yang dapat memperbaiki struktur jantung pada kondisi defisiensi protein
Paparan Pestisida Menginduksi Senesen Dini pada Mesenchymal Stem Cell In Vitro Adaninggar, Ascarti; Oktavya, Galuh; Putri, Raden Roro Risang Ayu Dewayani; Aprilianti, Suci Hari Utami; Nuriliani, Ardaning
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 1 (2024): February 2024
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i1.6340

Abstract

Pestisida merupakan senyawa kimia yang banyak digunakan sebagai pengendali hama pada aktivitas pertanian. Residu penggunaan pestisida ini dapat mengakibatkan polusi pada lingkungan perairan khususnya di sekitar lahan pertanian. Pestisida yang terakumulasi pada lingkungan perairan maupun hasil pertanian memberikan dampak buruk bagi manusia antara lain gangguan pada sistem organ, jaringan, perkembangan, dan pada tingkat sel mengarah ke senesen. Senesen merupakan suatu kondisi saat sel berhenti melakukan proliferasi. Sel yang mengalami senesen secara alami umumnya terjadi pada individu tua, sebagai respons terhadap pemendekan telomer. Senesen dini akibat paparan pestisida pada umumnya melibatkan mekanisme stres oksidatif, kerusakan DNA, dan disfungsi mitokondria. Senesen memicu penurunan fungsi organ yang mengakibatkan berbagai masalah kesehatan seperti kanker, osteoporosis, penyakit kardiovaskuler hingga demensia. Selain itu, senesen juga dapat menyebabkan berhentinya siklus sel punca antara lain pada mesenchymal stem cells (MSCs). Ulasan ini fokus membahas mekanisme senesen akibat paparan pestisida pada sel punca terutama MSCs. Metode yang digunakan yaitu koleksi data dan analisis dari jurnal terindeks Scopus dengan menggunakan VOSviewer. Berdasarkan hasil ulasan diketahui bahwa pestisida menginduksi senesen pada MSCs melalui jaras peningkatan konsentrasi ROS dalam sel dan penurunan aktivitas ALDH. Hal tersebut menyebabkan aktivasi p53, dan p21, yang kemudian akan menyebabkan hambatan pada CDK2 dan  pRB, berakibat pada  inaktivasi E2F serta induksi senesen. Senesen juga akan memberikan respons patofisiologis lain hingga efek tumorigenesis. 
INVENTORY AND STUDY PHARMACOLOGICAL POTENTIAL OF PLANTS IN THE PAJANGAN HAMLET, WEDOMARTANI, NGEMPLAK, SLEMAN, YOGYAKARTA Utami, Zidni Meirizka; Rahayu, Fadiah Sri; Yuvita, Arneta; Wardani, Ester Dewanti Yovita; Artik, Arba' Ramadhani; Nuriliani, Ardaning; Sudarsi, Nurini Aru
Jurnal Pendidikan Matematika dan IPA Vol 15, No 2 (2024): May 2024
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/jpmipa.v15i2.75245

Abstract

This research aims to inventory and study pharmacological potention of plants in Pajangan Hamlet, Wedomartani, Ngemplak, Sleman, Yogyakarta as medicinal plants and live stalls. The research was conducted from June to October 2023 using observational methods. Plants were identified with PlantNet software and reference againts the Flora of Java book. In addition, the study of the utilization of these plants was carried out by literature study and distributing questionnaires to the residents. The results found 104 plant species with 19 different families can be identified in Pajangan Hamlet. The plants are categorized as herbs, ornamental plants/flowers, fruits, vegetables, and wild plants. These findings highlight the richness of biodiversity in Pajangan Hamlet with significant potentital for improving health and economic livelihoods through the use of local plant species in traditional practices and diets. Based on the result, it can be concluded that Pajangan Hamlet has a variety of plants that are widely used in traditional medicine and food, thereby underscoring the importance of local biodiversity in improving health and well-being.
Effect of Black Rice Bran ‘Sembada Hitam’ on T47D Breast Cancer Cells: Effect of Black rice bran on T47D Cios Conara , Flafiani; Oktavya, Galuh; Asih Purwestri, Yekti; Nuriliani, Ardaning
Journal of Tropical Life Science Vol. 13 No. 3 (2023)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.13.03.17

Abstract

Indonesians have a rich tradition of incorporating black rice into their diet as a functional food due to its high antioxidant content. This research investigates the effects of the ethanolic extract of "Sembada Hitam" black rice bran on T47D breast cancer cells, specifically its cytotoxic, cell growth, and apoptotic induction properties. The MTT assay method was used to evaluate the cytotoxicity of the black rice bran extract on T47D cells after 24 and 48 hours of incubation. The acridine orange/propidium iodide (AO/PI) dye double staining method was employed to assess the apoptosis-inducing properties of the extract. Additionally, a cell growth assay was conducted to evaluate the effect of the extract on cell growth, with observations recorded on days 0, 3, and 6. The results revealed that the ethanolic extract of black rice bran "Sembada Hitam," when administered at various concentrations ranging from 7.81 to 1000 µg/mL, did not exhibit cytotoxic effects on T47D cells during the 24 and 48 hours of incubation. The cell growth assay revealed that T47D cells treated with the ethanolic extract of "Sembada Hitam" black rice bran at concentrations of 250 and 500 µg/mL exhibited lower growth rates than the 1% DMSO group from the 3rd to 6th day after incubation. The ethanolic extract of "Sembada Hitam" at concentrations of 250, 500, and 1000 µg/mL resulted in cell death of 10.64 ± 2.98%, 9.99 ± 5.87%, and 5.84 ± 0.78%, respectively. In conclusion, this study found that the ethanolic extract of "Sembada Hitam" black rice bran did not demonstrate significant cytotoxic effects on T47D breast cancer cells and is, therefore, unlikely to be a suitable candidate for an anti-cancer agent.
Extract of Black Rice (Oryza sativa L. ‘Sembada Hitam’) Bran Protect Cytotoxicity of Hydrogen Peroxide on Vero Cells in a Short Time Incubation: Black rice bran ‘Sembada Hitam’ protect cytotoxicity of H2O2 Aprilianti, Suci Hari; Purwestri, Yekti Asih; Saragih, Hendry T.S.S.G; Nuriliani, Ardaning
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.01

Abstract

Oxidative stress induced by hydrogen peroxide (H2O2) can lead to cellular damage, contributing to degenerative diseases and aging. Black rice bran is a functional food known for its antioxidant properties, which are crucial in reducing the adverse effects of oxidative stress and maintaining redox balance. In this study, we aimed to investigate the protective effect of the extract of black rice bran (EBRB) 'Sembada Hitam' on Vero cells against H2O2 toxicity. To evaluate the protective effect, a co-culture method was employed, and cell viability was assessed using the MTT assay. Additionally, cell growth was examined through trypan blue staining. Vero cells were exposed to different concentrations of H2O2 and EBRB for a 24-hour period. The results demonstrated that EBRB at concentrations of 15.625, 250, and 500 μg/mL exhibited a protective effect on Vero cells exposed to H2O2 at concentrations of 100, 200, and 400 μM, respectively. Notably, when Vero cells were treated with EBRB at concentrations of 250 or 500 μg/mL for five days in conjunction with H2O2 exposure at concentrations of 200 or 400 μM for 24 hours, a significant decrease in cell viability was observed on day 3. Based on the collective findings, it can be concluded that EBRB has the potential to protect Vero cells against H2O2 -induced toxicity, but primarily during a short-term incubation period. Overall, this study highlights the protective properties of EBRB against H2O2 -induced cellular damage and emphasizes the importance of further investigations to fully elucidate the underlying mechanisms and potential long-term effects of EBRB on cell viability.
Anticancer Potential of Ethanolic Extract Artocarpus heterophyllus Lam. Leaves against Human Colon Cancer WiDr Cell Line Mutiyani, Novita; Nuriliani, Ardaning
Biology, Medicine, & Natural Product Chemistry Vol 13, No 1 (2024)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2024.131.221-226

Abstract

Jackfruit (Artocarpus heterophyllus Lam.) is a plant contains various compounds that has potential as anticancer drugs. Secondary metabolites of jackfruit leaves are flavonoids, alkaloids, tannins, triterpenoids, and saponins. A typical flavonoid compound group in the Artocarpus genus, namely artocarpin, is able to kill cancer cells through apoptosis. Not many have conducted cytotoxicity research and apoptosis induction of ethanolic extracts from jackfruit leaves, especially against WiDr colon cancer cells. Therefore, this study aims to study the cytotoxic effects and apoptosis induction of ethanolic extracts of jackfruit leaves against WiDr colon cancer cells. The research was conducted by cytotoxicity test using the MTT assay. Apoptosis test was done using double staining method with AO/PI dye. The treatment was conducted at various concentrations of ethanolic extract of jackfruit leaves, doxorubicin as positive control, and DMSO as solvent control. The data were analyzed by one way ANOVA (p ≤ 0.05) and continued using Tukey HSD Post Hoc test. The results showed that the ethanolic extract of jackfruit leaves was not toxic to WiDr cells with an IC50 740.43 μg/mL, but could significantly reduce cell viability at a concentration of 500 μg/mL. The ethanolic extract of jackfruit leaves could also induce apoptotic cell death at a concentration of 500 μg/mL. Based on these results, ethanolic extract of jackfruit leaves has little potential to be developed as an anticancer drug.
Therapeutic Effects of BRC Functional Food from Indonesian Black Rice on Body Weight and Haematological Parameters in Obese Rats Sofyantoro, Fajar; Syam, Adi Mazdi; Adania, Baik Aisyah; Almunawar, Muhammad Fikri; Nasution, Nurlita Putri Bela; Hidayat, Rheina Faticha Asyamsa; Mataram, Made Bagus Auriva; Maharesi, Chesa Ekani; Nurhidayah, Septika; Purwestri, Yekti Asih; Nuriliani, Ardaning; Hidayati, Lisna; Pratiwi, Rarastoeti
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.85847

Abstract

Obesity increases the risk of various diseases. Black rice, renowned for its high anthocyanin content, is considered a potential functional food for preventing metabolic disorders. The current study investigated the effects of black rice crunch (BRC) on body weight and haematological profiles in obese rats. Rats were fed with high-fat diet to induce obesity and supplemented with different concentrations of BRC for 4 and 8 weeks. The results showed that high-fat diet effectively induced obesity, as evidenced by significant increase in body weight. Importantly, 75% BRC supplementation resulted in significant weight reduction in obese rats. Further analysis revealed an increase in erythrocyte numbers in obese groups supplemented with 75% BRC, but no significant changes in haemoglobin concentration or haematocrit percentage. Further investigation showed that 75% BRC led to a decrease in mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), and mean corpuscular volume (MCV), potentially affecting the size and concentration of haemoglobin within erythrocytes. The total leucocytes count increased with the high-fat diet, while BRC supplementation alone did not have significant impact. Lymphocyte percentage remained stable across the groups, indicating minimal influence of the dietary interventions. Neutrophil percentage varied initially but was not specific to BRC or the high-fat diet. Platelet count and distribution width were not significantly influenced, but mean platelet volume (MPV) increased after 8 weeks of BRC treatment, suggesting larger platelet sizes associated with obesity. Overall, the study provides important insights into the effects of BRC supplementation on body weight and haematological parameters related to obesity. 
Mekanisme Penyakit Kardiovaskular terkait Penuaan Nuriliani, Ardaning; Apriliyani, Tia; Pusparini, Nur Ainun Oktavia; Karmilah, Karmilah; Rohmah, Zuliyati; Tunjung, Woro Anindito Sri
Bioma : Berkala Ilmiah Biologi Vol. 26 No 2, Tahun 2024
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.2024.59115

Abstract

Penuaan merupakan proses patofisiologis ditandai dengan penurunan fungsi sel dan jaringan yang menyebabkan peningkatan risiko penyakit terkait penuaan, salah satunya penyakit kardiovaskular. Kajian pustaka ini bertujuan untuk mempelajari mekanisme beberapa penyakit kardiovaskular seperti hipertensi, aritmia, dan stroke. Data kajian berupa data sekunder yang berasal dari jurnal dan artikel tanpa batasan tahun terbit. Basis data yang digunakan yaitu Science direct, google scholar, NCBI, scopus, dan web. Kata kunci yang digunakan untuk pencarian buku, jurnal, dan artikel adalah hipertensi, hypertension mechanism, hypertension factor, stroke diseases, stroke mechanisms, risk factor causes stroke, stroke molecular mechanisms, arrhythmia, arrhythmia disease, arrhythmia mechanism, risk factor of arrhythmia, risk factor of arrhythmia. Hipertensi disebabkan oleh ROS  yang mengikat nitrat oksida (NO) menghasilkan peroksinitrit (ONOO2) sehingga terjadi disfungsi endotel. Aritmia terjadi akibat kondisi inflamasi yang mengaktivasi oksidase NADPH menyebabkan perkembangan fibrilasi atrium kronis. Stroke terjadi akibat penyempitan pembuluh darah yang menyebabkan akumulasi sel senesen dan menginduksi peradangan serta penuaan endotel. Penyakit kardiovaskular seperti hipertensi, aritmia, dan stroke dapat disebabkan oleh mekanisme yang berkaitan dengan penuaan.
Co-Authors Abdul Rachman Adania, Baik Aisyah Adaninggar, Ascarti Adelfiani, Adelfiani Adi Susanto Adi Susanto Adi Susanto Aeniah, Siti Agus Hermawan Allimi, Hayu Swari Almunawar, Muhammad Fikri Andi Mahendra Andriyani, Eka Fitri Siti Anisya, Alifia Anita Restu Puji Raharjeng Aprilianti, Suci Hari Aprilianti, Suci Hari Utami Apriliyani, Tia Ariesti, Wiwin Arif Luthfi Nurul Huda Artik, Arba' Ramadhani Ascarti Adaninggar Ashfiya Hanif Hasnadewi Asih Purwestri, Yekti Aswi A. Rofiqoh Bambang Retnoaji Bima Mahendra Bima Mahendra Bima Mahendra Cios Conara , Flafiani Conara, Flafiani Cios Dafa, Maula Haqul Della Blatama Empra, Desi Eka Putri Fajar Sofyantoro Faulana, Arum Fahmi Firdiana, Vykra Aulia Flafiani Cios Conara Flafiani Cios Conara Flafiani Cios Conara Gunanegara, Rimonta Febby Halim, Shuha Ma’muriyah Haris Setiawan Hayu Swari Alimi Hendry Saragih Hendry Saragih Hendry Saragih Hendry T.S.S.G. Saragih Hendry T.S.S.G. Saragih Hendry Tri Sakti Saragih Hendry Tri Sakti SG Saragih Hidayah, Lailly Tsania Nur Hidayat, Rheina Faticha Asyamsa Ibnu Agus Ariyanto Illiyin, Sirayya Jessy Octavia Harahap Johanes Sugiyanto Karlina, Ina Karmilah, Karmilah Laili Mufli Zusrina Lailly Tsania Lailly Tsania Nur Hidayah Lailly Tsania Nur Hidayah Larasshita Putri Adhitya Sani Lisna Hidayati, Lisna Luthfi Nurhidayat Maharesi, Chesa Ekani Manesta Edelweis Jingga Mataram, Made Bagus Auriva Mayani, Olvita Mei Ria Santi Mulyati Mulyati Murtiadi Erlan Supraitno Mutiyani, Novita Nastiti Wijayanti Nasution, Nurlita Putri Bela Ni Wayan Erly Sintya Dewi Nurhidayah, Septika Nurhidayat, Luthfi Oktaviana, Shintia Oktavya, Galuh Pertiwi, Anggita Endar Podhi, Felisitas Moli Prasasti, Filosofia Florista Tesla Aulia Pratiwi, Arini Dian Primahesa, Alfian Prissandi, Anthera Al Firdaus Pusparini, Nur Ainun Oktavia Puteri, Nabila Ramiza Putri, Raden Roro Risang Ayu Dewayani Rahayu, Fadiah Sri Rahayu, Sekar Rahmadian, Desti Rarastoeti Pratiwi Retno Wulandari Ria Vena Septhay Sari Roesma Narulita Rohmah, Zuliyati Rumansara, Papuani Samparisna Ruth Liananda Citra Dolok Saribu Sadewo, Imran Sadiman Sadiman Saeed, Faisal Sandi Francisco Pratama Saragih, Hendry Saragih, Hendry T. S. S. G. Saragih, Hendry T.S. Saragih, Hendry T.S.S.G Saragih, Hendry Tri Sakti SG Sari, Dini Wahyu Kartika Saribu, Ruth Liananda Citra Dolok Sari’ah Cintami Damayanti Septriani, Nur Indah Septriani, Nur Indah Setyo, Agnessia Asian Budi Shalihah, Fianicha Simanungkalit, Eben Ezer Siwi Dwi Rahayu Slamet Widiyanto Sudarsi, Nurini Aru Sularsah, Sais Sunarti Susilo Hadi, Susilo Syam, Adi Mazdi Tia Apriliyani Utami, Zidni Meirizka Wardani, Ester Dewanti Yovita Woro Anindito Sri Tunjung Yekti Asih Purwestri Yuny Erwanto Yuvita, Arneta Zeyna Listy Zulfatin Zuliyati Rohmah Zusrina, Laili Mufli