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The Anti-proliferation Effect of an Isolated Butanol Fraction of Tampa Badak (Voacanga foetida (Bl.) K. Schum) Leaves on Leukemia, Lung, and Cervical Cancer Susanty, Adriani; Dachriyanus, Dachriyanus; Yanwirasti, Yanwirasti; Sri Wahyuni, Fatma; Sekar, Andisyah Putri; Alimin, Nur; Magdazaleni, Magdazaleni; Sofia, Sri Esky; Dewi, Citra Kartika
Pharmaceutical Sciences and Research Vol. 7, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Voacanga foetida (Bl.) K.Schum leaves are known to have cytotoxic activity against blood cancer cells. This study was aimed to determine the potential of an isolated butanol fraction of Voacanga foetida (Bl.) K.Schum leaves (Tb3 compound) in inhibiting the proliferation of several cancer cells, including leukemia (K562), lung cancer (A549), and cervical cancer (He-La) using the dye exclusion method. We found that the percentage of anti-proliferation of the Tb3 compound was increased in a dose-dependent manner, where it showed 83% (K562), 72.3% (He-La), and 70.7% (A549) inhibition at higher concentration. These values were relatively higher compared to doxorubicin as a positive control, 77.1%, 63.8%, and 62.7%, respectively. Moreover, the IC50 values of the Tb3 compound were still in the range of active cytotoxic compounds based on NCI classification, which is less than 4 µg/mL; 0.5 μg/mL, 2.4 μg/mL, and 3.6 μg/mL for K562, A549, and He-La, respectively. Therefore, this study indicated the potential anticancer effect of an isolated butanol fraction of the Tb3 compound to inhibit cancer cell proliferation, which provides an alternative for cancer therapy.
Anti-Inflammatory Effect of Isolate Compounds from Ethyl Acetate Fraction of Piper crocatum Ruiz and Pav Leaves on Lipopolysaccharide-induced RAW 264.7 Cells Nilda Lely; . Dachriyanus; Yufi Aldi; . Almahdy; Fatma Sri Wahyuni
HAYATI Journal of Biosciences Vol. 30 No. 1 (2023): January 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.1.28-34

Abstract

Red betel (Piper crocatum Ruiz and Pav) is a traditional Indonesian plant. The ethyl acetate fraction of red betel leaf has the potential as an anti-inflammatory. This research aimed to determine the anti-inflammatory effect of isolate compound from ethyl acetate fraction of Piper crocatum leaves on the production of cytokine pro-inflammatory TNF-α and ICAM-1 in lipopolysaccharide-induced RAW 264.7 cells. Cell viability was determined by the MTT method in the concentration range of 1.25; 2.5; 5; 10; 20, and 40 µg/ml. The TNF-α and ICAM-1 level was determined by the TNF-α and ICAM-1 assay kit as measured by an ELISA plate reader. Cell viability test of isolate compound from ethyl acetate fraction, the concentrations that gave viability percentages above 80% were 10; 5; 2.5 and 1.25 ug/ml. Isolate compound at concentrations of 10, 5, 2.5 and 1.25 ug/ml significantly inhibited TNF-α and ICAM-1 production with p<0.05 (p = 0.000).
PENGEMBANGAN TANAMAN OBAT KELUARGA DI PANTI ASUHAN RAHMATAN LIL ALAMIN SAWAHAN KECAMATAN PADANG TIMUR KOTA PADANG Elidahanum Husni; Dira Hefni; Dachriyanus Dachriyanus; Netty Suhatri; Meri Susanti
BULETIN ILMIAH NAGARI MEMBANGUN Vol 5 No 4 (2022)
Publisher : LPPM (Institute for Research and Community Services) Universitas Andalas Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/bina.v5i4.462

Abstract

Indonesia is a rich country with biodiversity consisting of thousands of medicinal plant species and approximately 300 species are used as traditional medicine by the traditional medicine industry. Therefore, the existing biodiversity in Indonesia is an asset and resource that must be maintained and managed so that it can become an ancestral heritage and benefit the community for maintaining health. FMP (Family Medicinal Plants) is a type of plant of choice that is efficacious as medicine with easy maintenance and relatively low cost. FMP is a safe alternative for family medicine because it rarely causes side effects, is easy to process, and is consumed for first aid in cases of minor illnesses such as fever, cough, or to help maintain stamina. The existence of FMP in the home environment is very important because apart from being an alternative treatment it can also improve family welfare. The purpose of carrying out this community service activity is that the residents of the Rahmatan Lil Alamin Orphanage know the benefits, how to use, and how to make traditional dosage form from FMP. The method used in this activity is providing counseling, training, giving FMP seeds, and tools that can be used to make products of traditional medicinal plants. In counseling activities, pretest and posttest sheets were also given. The results of the service showed that the average pretest score of the participants was 85 and increased in the post test to 94. It can be concluded that the participants took part in the activity enthusiastically so that their knowledge of the Family Medicinal Plants (FMP) increased.
Analisis Rendang Daging Sapi dan Daging Babi Hutan Menggunakan Metode Spektroskopi FTIR Kombinasi Kemometrik untuk Autentikasi Halal Regina Andayani; Ditya Kesumaningrum; Tadzkia Nisa; Elidahanum Husni; Suryati Suryati; Syofyan Syofyan; Dachriyanus Dachriyanus
Jurnal Sains Farmasi & Klinis Vol 10, No 1 (2023): J Sains Farm Klin 10(1), April 2023
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jsfk.10.1.78-88.2023

Abstract

ABSTRAKRendang merupakan salah satu makanan tradisional Sumatra Barat dari olahan daging sapi. Harga daging sapi yang mahal, mendorong pemalsuan menggunakan daging babi hutan. Penelitian ini bertujuan untuk mengembangkan metode analisis autentikasi rendang sapi menggunakan spektroskopi FTIR kombinasi kemometrik. Sampel yang digunakan adalah campuran daging sapi dan babi hutan yang dibuat dalam 11 konsentrasi (training data set) serta rendang rumah makan (testing data set). Komponen lemak rendang diekstraksi menggunakan metode Folch dan Bligh Dyer kemudian dianalisis dengan spektroskopi FTIR. Spektra FTIR yang dihasilkan digunakan sebagai variabel pemodelan kemometrik. Untuk kuantifikasi, model PLS di bilangan gelombang 1250-950 cm-1 memberikan model terbaik pada metode Folch dengan nilai R2 kalibrasi 0,9946, R2 prediksi 0,9954, RMSEC 0,0328,  RMSEP 0,0402 dan pada metode Bligh Dyer, model PCR di bilangan gelombang 1800-500 cm-1 memberikan model terbaik dengan nilai R2 kalibrasi 0,9940, R2 prediksi 0,9919, RMSEC 0,0345, RMSEP 0,0457. Untuk klasifikasi, model DA di daerah gabungan bilangan gelombang 1800-1600 cm-1 dan 1250-950 cm-1 dengan metode Folch dan bilangan gelombang 1800-650 cm-1 dengan metode Bligh Dyer, berhasil mengelompokkan training data set menjadi beberapa kelas dan mengelompokkan testing data set ke dalam kelas sapi. Dengan demikian disimpulkan bahwa analisis spektroskopi FTIR kombinasi kemometrik merupakan metode screening yang cepat, sederhana dan murah untuk autentikasi rendang sapi dan babi hutan.Kata kunci: autentikasi, rendang sapi, babi hutan, FTIR, kemometrik ABSTRACTRendang is one of West Sumatra's traditional foods made from processed beef. The high price of beef encourages counterfeiting using wild boar meat. This study aims to develop a method of authentication analysis of beef rendang using FTIR spectroscopy in combination with chemometrics. The sample used is a mixture of beef and boar meat made in 11 concentrations (training data set) and restaurant rendang (testing data set). The rendang fat component was extracted using the Folch and Bligh Dyer methods and then analyzed by FTIR spectroscopy. The resulting FTIR spectra are used as a chemometric modelling variable. For quantification, the PLS model at wave number 1250-950 cm-1 gives the best model in the Folch method with a calibration R2 value of 0.9946, prediction R2 of 0.9954, RMSEC 0.0328, RMSEP 0.0402 and in the Bligh Dyer method, the model PCR at wavenumber 1800-500 cm-1 gave the best model with a calibration R2 value of 0.9940, prediction R2 of 0.9919, RMSEC 0.0345, RMSEP 0.0457. For classification, the DA model in the combined area of wave numbers 1800-1600 cm-1 and 1250-950 cm-1 with the Folch method and wave numbers 1800-650 cm-1 with the Bligh Dyer method, successfully grouped the training data set into several classes and grouped testing data set into cow class. Thus it was concluded that FTIR spectroscopic analysis combined with chemometrics is a fast, simple, and inexpensive screening method for the authentication of beef rendang and wild boar.Keywords: authentication, beef rendang, wild boar, FTIR, chemometrics 
PEMBUATAN PRODUK SABUN CAIR DARI ECO-ENZYME DI KELURAHAN ANDALAS KECAMATAN PADANG TIMUR KOTA PADANG Purnawan Pontana Putra; Fatma Sri Wahyuni; Yelly Oktavia Sari; Erizal Erizal; Dachriyanus Dachriyanus; Yufri Aldi; Dedy Almasdy; Salman Salman
Jurnal Hilirisasi IPTEKS Vol 6 No 1 (2023)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jhi.v6i1.644

Abstract

Eco-Enzyme, a multifunctional liquid produced from a mixture of organic waste such as vegetables, fruits, brown sugar, coconut sugar, corn sugar, sugarcane, and water that has undergone fermentation, has various benefits for the environment and human health. Eco-Enzyme contains beneficial enzymes that accelerate biochemical reactions in the environment. The enzymes found in Eco-Enzyme can be used for various applications, such as organic liquid fertilizer for plants, an additive in detergents, floor cleaners, pesticide residue cleaners, and rust removers. Using Eco-Enzyme as a liquid soap can help reduce soil pollution. Meanwhile, using Eco-Enzyme as a cleaning agent can help reduce the use of hazardous chemicals that damage the environment. This integrated program is carried out in three stages: the first is to socialize the benefits of using Eco-Enzyme, the second is to make Eco-Enzyme, and the third is to make soap from Eco-Enzyme. The production of Eco-Enzyme begins with the collection of organic waste consisting of fruit and vegetable waste, which is then mixed with brown sugar and water and left to ferment for three months. After the Eco-Enzyme product is ready, soap-making uses ingredients such as potassium hydroxide (KOH), coconut oil, cooking oil, distilled water, orange-scented perfume, and Eco-Enzyme. Training on Eco-Enzyme and soap making is conducted in the Andalas Subdistrict, which is welcomed enthusiastically by the local community. This program is expected to serve as a reference for transforming organic waste into more practical and environmentally friendly sustainable products.
PENGEMBANGAN TANAMAN PEMANIS STEVIA REBAUDIANA (BERTONI) DI EKOWISATA SUNGKAI PARK LAMBUNG BUKIT KECAMATAN PAUH KOTA PADANG Elidahanum Husni; Dira Hefni; Dachriyanus Dachriyanus; Netty Suhatri; Meri Susanti
BULETIN ILMIAH NAGARI MEMBANGUN Vol 6 No 1 (2023)
Publisher : LPPM (Institute for Research and Community Services) Universitas Andalas Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/bina.v6i1.461

Abstract

The human need for sugar as an additional ingredient to give sweet taste to food and drinks is very high. However, excessive consumption of sugar can cause problems, especially for people with obesity and diabetes. Therefore, an alternative sweetener is needed to replace sugar which does not have harmful effects on health and is low in calories, one of which is stevia sweetener. The purpose of this activity is for the community/medicinal plant farmers in the Sungkai Park Memorial Area to know about the types of plants that have health benefits such as the Stevia plant as a natural sweetener that can be consumed for health and can be stored for a long time. The activity was carried out at the Self-Help Agriculture and Rural Training Center (P4S) in Sungkai Village, Sungkai Green Park Ecotourism, Lambung Bukit Village, Padang City. This activity was carried out in three stages, namely: 1. counseling and training on how to make good quality traditional medicine namely Stevia, 2. practice of making Stevia leaf extract products, and 3. evaluation. The results of the implementation of the activity were that it could increase public awareness and awareness of the use of Stevia leaves in making health drinks. Stevia is obtained from the Stevia rebaudiana plant and is safe to consume in moderate doses of 0.1-4 mg per kilogram of body weight per day. stevia has many advantages compared to sugar, including 300 times sweeter than sucrose, does not damage teeth, can lower blood pressure and does not increase blood sugar levels. In addition, stevia can increase blood insulin levels, although the increase is relatively small. In addition to its use as a substitute for sugar as a sweetener. It is hoped that Stevia will become a natural sweetener that can be consumed for health and can be accepted by the market so that it can improve the community's economy, especially from the agricultural sector.
Cytotoxic Activity of Ethyl Acetate Extract from Voacanga foetida (Bl.) Rolfe Leaves Against T47D Breast Cancer Cells Adriani Susanty; Mira Febrina; Dian Sanita Putri; Ihsan Ikhtiarudin; Fatma Sri Wahyuni; Dachriyanus Dachriyanus
Borneo Journal of Pharmacy Vol. 6 No. 1 (2023): Borneo Journal of Pharmacy
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjop.v6i1.3499

Abstract

The cytotoxicity of ethyl acetate extract from Tampa badak (Voacanga foetida (Bl.) Rolfe) leaves against Artemia salina leach larvae was determined using the brine shrimp lethality test (BSLT) method and was evaluated against T47D breast cancer cells using MTT assay method. The result of BSLT showed a consistent result with MTT assay, which is the result obtained that ethyl acetate extract is very toxic against A. salina Leach larvae with LC50 value of 8.61 µg/mL and very cytotoxic against T47D breast cancer cells with IC50 values of 0.87; 0.66; and 0.95 µg/mL at the 24, 48 and 72 hours of incubation times, respectively. The MTT assay data were analyzed using a two-way ANOVA statistical method to see the effect of the dependent variable (concentration and time) on the independent variable (% viability). Based on the statistical test result, there is a difference in % viability between concentrations of 0.1; 1; and 10 µg/mL (p <0.05), but the length of incubation does not affect % viability (p >0.05).
Uji Senyawa Bioaktif Dan Toksisitas Limbah Cangkang Kerang Hijau (Pera Viridis) Suhaera Suhaera; Aprilya Sri Rachmayanti; Shinta Sari Dewi; St. Mursyidah; Dachriyanus Dachriyanus
Jurnal Ilmu Kedokteran dan Kesehatan Indonesia Vol. 1 No. 3 (2021): NOVEMBER : Jurnal Ilmu Kedokteran dan Kesehatan Indonesia
Publisher : Pusat Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/jikki.v1i3.2932

Abstract

Green mussels are organisms belonging to the class pelecypoda, this class has two bilaterally symmetric valve shells, these green mussels are referred to as bivalves, and are included in the soft-bodied marine biota (Mollusca). This study aims to determine the bioactive compounds and toxicity contained in the shells of green mussels (Perna viridis) which are processed seafood typical of the Riau Islands. Secondary metabolite screening tests include alkaloids, flavonoids, tannins, saponins, steroids and terpenoids as for toxicity tests using the BSLT (Brine Shrimp Lethality Test) method with shrimp larvae (Artemia salina Leach) test animals. The results of secondary metabolite screening on the test for alkaloids, flavonoids, tannins, steroids and terpenoids were declared negative while the saponin test was positive with the formation of stable foam. The results of the BSLT toxicity test (Brine Shrimp Lethality Test) with concentrations of 10 ppm, 100 ppm, and 1000 ppm obtained LC50 <1000 g/ml, which is 1127.28 ppm in the non-toxic category. In the sense that it is safe to be used as processed food.
Isolation and Cytotoxic Activity Test of Alkaloids from Dichloromethane Fraction of Bark of Tampa Badak (Voacanga foetida (Blume) Rolfe) Against T47D Cell Line Adriani Susanty; Nurdina Putri; Ihsan Ikhtiarudin; Novia Sinata; Dira Dira; Fatma Sri Wahyuni; Dachriyanus Dachriyanus
Journal of Tropical Pharmacy and Chemistry Vol. 7 No. 2 (2023): J. Trop. Pharm. Chem.
Publisher : Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia, 75117, Gedung Administrasi Fakultas Farmasi Jl. Penajam, Kampus UNMUL Gunung Kelua, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jtpc.v7i2.583

Abstract

Cancer is a problem in the health sector because this disease has an increasing incidence of morbidity and mortality. While the treatment still leaves some drug side effects, which cause discomfort for cancers sufferers. The arises because most cancer drugs work non-selectively. Because of some of the problems above, it is necessary to encourage the search for alternative drugs treatment of cancer. Voacanga foetida (Blume) Rolfe (Apocynaceae) is a species of plant from the genus Voacanga known as the tampa badak from West Sumatra. This plant is known to be source of indole alkaloids and also bis-indole alkaloids potential as cancer drugs. Therefore, in this study we are interested to explore the potency of this plant as natural product sources with potential cytotoxic activity. The study was started from the isolation process and continued by characterization of the isolated compounds and then testing their cytotoxic activity T47D breast cancer cell line. The isolation process of alkaloid compounds were performed by acid-base extraction, liquid-liquid fractionation, and followed by separation using column chromatography. Then,the potency of cytotoxic activity of the isolates were screened through Brine Shrimp Lethality Test (BSLT) method and evaluated by MTT assay. The structure of three isolated alkaloids (VFB-DB1.1; VFB-DB2.1; and VFB-DB2.2) were charachterized using spectroscopic analyses, including UV and FT-IR. One isolated compound (VFB-DB1.1) was also characterized using 1H NMR spectroscopy. Based on the similarity of 1H NMR spectra of isolated compound when compared with the literature, compound VFB-DB1.1 was identified as voacangine. Then, the BSLT and MTT assay result showed that VFB-DB1.1 exhibited LC50 value of 15.8 µg/mL againt Artemia salina L.and IC50 value of 8.9 µg/mL against T47D breast cancer cells, respectively.
Penentuan Kadar Fenolat Total, Uji Aktivitas Antioksidan dan Antibakteri dari Ekstrak dan Fraksi Kulit Batang Bintangor (Calophyllum soulattri Burm. F) Husni, Elidahanum; Dachriyanus, Dachriyanus; Saputri, Veny Wahyu
JSFK (Jurnal Sains Farmasi & Klinis) Vol 7 No 1 (2020): J Sains Farm Klin 7(1), April 2020
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jsfk.7.1.92-98.2020

Abstract

Bintangor (Calophyllum soulatri Burm. F) merupakan tumbuhan dari familia Clusiaceae. Beberapa bagian dari tumbuhan ini seperti kulit batangnya telah lama digunakan sebagai obat tradisional. Penelitian ini bertujuan untuk mengetahui kadar fenolat total, aktivitas antioksidan dan antibakteri dari kulit batang bintangor dalam bentuk ekstrak dan fraksi. Ekstrak kulit batang bintangor dibuat dengan teknik maserasi dengna pelarut etanol, sedangkan fraksinasi dilakukan dengan pelarut n-heksan, etil asetat dan butanol. Kadar fenolat total dari ekstrak dan fraksi ditentukan menggunakan metode Folin-Ciaocalteau. Aktivitas antioksidan ditentukan dengan metode Ferric Reducing Antioxidan Power, sedangkan aktivitas antibakteri dievaluasi dengan metode difusi agar terhadap bakteri patogen. Hasil penelitian menunjukkan bahwa kadar fenolat total tertinggi diperoleh dari ekstrak etanol (60,481 g/100 g), demikian juga dengan aktivitas antioksidan tertinggi (4,29 mmol Fe(II)/100 g). Aktivitas antibakteri paling baik diperlihatkan oleh ekstrak etanol dengan konsentrasi hambat minimum 0,8%, menghasilkan diameter hambat 6,96 mm, diikuti oleh fraksi n-heksan (konsentrasi 0,9%, menghasilkan diameter hambat 6,63 mm), fraksi etil asetat (konsentrasi 0,625%, menghasilkan diameter hambat 6,28 mm), dan fraksi butanol (konsentrasi 0,5%, menghasilkan diameter hambat 6,03 mm).
Co-Authors ', Darwati ', Supriyatna . Almahdy . Ellyza . Yanwirasti A. Manaf Ali AA Sudharmawan, AA Abd. Rasyid Syamsuri Abdul Rohman Abu Bakar, Nor Kartini Adriani Susanty Agung Tri Sasongko Alimin, Nur Almahdy A Almahdy, . Amella Gusty Amri Bachtiar Andayani, Regina Andryani, Putu Cisya Aprilya Sri Rachmayanti Darwati - Dewi, Citra Kartika Dian Dwiana Maydinar Dian Handayani Dian Sanita Putri Dira Dira Dira Hefni Ditya Kesumaningrum Dwisesaria Irma Lena Eka Roza Wijaya Ekaprasada, M. Taufik Elfita Elfita Elfita Elfita Elfita Elfita Elfita Elidahanum Husni Ellis Mawarni Elyunaida, Elyunaida Erizal Erizal Faleh Setia Budi Fatma Sri Wahyuni Fitra Yeni Gusty, Amella Hadi Ihsan Hadi Nasbey Haiyul Fadhli Harmawati Harmawati Hazli Nurdin Hazli Nurdin Hazli Nurdin Hazli Nurdin Henny Lucida Hj. Lajisc, Nordin Hj. Lajisc, Nordin Husein Hernadi Bahti Husen H Bahti Husen H. Bahti Husen H. Bahti - I Gusti Bagus Wiksuana Ihsan Ikhtiarudin Jufri Al Fajri Kamal Rullah Kesumaningrum, Ditya Krisyanella Krisyanella Leni Merdawati Linda Eka Putri Lusianti, Mittha Lusianti, Mittha M. Taufik Ekaprasada M. Taufik Ekaprasada M. V. Sargent Madyawati Latief Magdazaleni, Magdazaleni Maisa, Esthika Ariany Masrul Md Nordin Hj Lajis Md Nordin Hj Lajis Meri Ardianti Meri Susanti Meri Susanti Miftahul Husna Mira Febrina Muharni Muharni Muharni Muharni Muharni Muharni N. H. Ladjis Nancy Dewi Yuliana Netty Suhatri Nilda Lely Nilda Tri Putri Nisa, Tadzkia Nordin Lajis Novi Safitri Noviatri Noviatri, Noviatri Noviyandi Sayuti Nurdin, Hazli Nurdin, Hazli Nurdina Putri Pedro Anugrah Aswan Putra, Deddy Prima Putra, Deddy Prima Rahma Yulia Rahmat, Rosita Dewi Rahmat, Rosita Dewi Regina Handayani Handayani, Regina Handayani Rizal Fahmi Rob W.M van Soest Rosfita Rasyid Rustini Rustini Salman - Sanusi Ibrahim Saputri, Veny Wahyu Sartika, Zikra Sartika, Zikra Saufitri, Dian Saufitri, Dian Sekar, Andisyah Putri Shinta Sari Dewi Sinata, Novia Sofia, Sri Esky St. Mursyidah Suhaera Suhaera Suhatri Suhatri Supriyatna - Supriyatna Soetardjo Supriyatna Supriyatna Supriyatna Supriyatna Supriyatna Supriyatna Supriyatna Supriyatna Supriyatna Supriyatna Surya Dharma Suryati Suryati . Suryati Suryati Suryati Suryati Suryati Suryati Suryatir - Syofyan Syofyan Tadzkia Nisa Titen Yasril Veny Wahyu Saputri Vetty Priscilla Wijaya, Eka Roza Yanwirasti Yanwirasti Yanwirasti Yanwirasti Yasman, Yulia Yelly Oktavia Sari Yohannes Alen Yufi Aldi Yufri Aldi Yulia Yasman Yunazar Manjang Yunazar Manjang