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EFFECTS OF HUMIC ACID ADDITION TO FEEDS WITH HEAVY METAL CADMIUM CONTAMINATION FROM GREEN MUSSELS ON THE GROWTH PERFORMANCE OF ASIAN SEABASS Rasidi, Rasidi; Jusadi, Dedi; Setiawati, Mia; Yuhana, Munti; Jr., Muhammad Zairin; Sugama, Ketut
BIOTROPIA Vol. 26 No. 3 (2019): BIOTROPIA Vol. 26 No. 3 December 2019
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (236.409 KB) | DOI: 10.11598/btb.2019.26.3.1114

Abstract

Although it contains heavy metals, the highly nutritious green mussel Perna viridis is used as a fish meal replacement in fish diets. Fortunately, humic acid (HA) has the ability to chelate heavy metals in animal feeds. Its addition to fish feed formulation is, therefore, needed to prevent the accumulation of heavy metals in the fish's body. Hence, an experiment using a Completely Randomized Design (CRD) with 5 treatments and 3 replications was conducted to evaluate the performance and feed efficiency of Asian seabass Lates calcarifer juveniles with the addition of HA to their green mussel–based diet containing the heavy metal cadmium (Cd). Five experimental diets were formulated with different dosages of HA addition at 0, 400, 800, 1200, and 1600 mg kg⁻¹ of feed as treatments A, B, C, D, and E, respectively. The experimental diets were given to triplicate groups of 15 Asian seabass juveniles with an initial body weight of 4.30 ± 0.60 g over a 70-day cultivation period, feeding until satiation. The results showed that feed consumption, feed digestibility, protein retention, growth performance, and feed efficiency were significantly affected by HA addition in the fish diet. Among all treatments, HA addition of 1600 mg kg⁻¹ produced the best biological response from the Asian seabass. To conclude, the HA addition of 1600 mg kg⁻¹ into the diet was the best dosage, resulting in optimal growth performance and feed efficiency in Asian seabass. The highest dose of HA in Cd-contaminated feeds could reduce Cd content, but had not yet been able to eliminate Cd in fish meat. Therefore, green mussel meal, with HA addition as an alternative protein source, has potential as a feed additive for Cd-contaminated diets of Asian seabass juveniles.
Utilization of mung bean sprout waste Vigna radiata hydrolyzed cellulase enzyme in feed on the digestibility of Nile tilapia Oreochromis sp. Audia Wuryas Pradita Negara, Grenda; Setiawati, Mia; Ekasari, Julie; Jusadi, Dedi
Jurnal Akuakultur Indonesia Vol. 25 No. 1 (2026): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.25.1.138-150

Abstract

This study evaluated the utilization of mung bean sprout waste flour hydrolyzed by cellulase enzyme (LTe) as the feed ingredient of red Nile tilapia weighing 10.00 ± 0.01 g/seed and 7.00 ± 0.15 in length. This study used two stages, each consisting of four treatments and four replications. The first step was performed by evaluating LTe flour added with cellulase enzyme of 0 g/kg (control), 0.4 g/kg, 0.8 g/kg, and 1.2 g/kg. The second step was the digestibility test of LTe and growth performance on red Nile tilapia seeds. The results showed that the addition of cellulase enzyme at a 1.2 g/kg was significantly able to reduce the crude fiber of LTe with 78.19% %, hemicellulose at 19.22%, neutral detergent fiber at 41.69%, acid detergent fiber at 61.85%, lignin 64.06%, cellulose 62.47% besides having the best value of ingredient, protein, and energy digestibility. The test results on the growth performance of red Nile tilapia seeds fed with LTe feed with a dose of 1.2 g/kg cellulase enzyme have the highest value significantly different from the control feed based on the value of daily growth rate (SGR), ratio efficiency protein (REP), protein retention (PR), and improvement of feed conversion ratio (RKP).   Keywords: cellulase enzyme, digestibility, growth performance, mung bean sprout waste, tilapia   Abstrak Penelitian ini mengevaluasi pemanfaatan tepung limbah kecambah kacang hijau yang dihidrolisis enzim selulase (LTe) sebagai bahan baku pakan pada benih ikan nila merah dengan bobot 10,00 ± 0,01 g/ekor dan panjang 7,00 ± 0,15 cm. Penelitian ini menggunakan dua tahap dan masing-masing tahap terdiri dari empat perlakuan dan empat ulangan. Tahap pertama dilakukan evaluasi tepung LTe sebesar 0 g/kg (kontrol), 0,4 g/kg, 0,8 g/kg, dan 1,2 g/kg. Tahap kedua dilakukan uji kecernaan bahan LTe dan kinerja pertumbuhan benih ikan nila merah. Hasil penelitian menunjukkan bahwa penambahan enzim selulase pada dosis 1,2 g/kg signifikan mampu menurunkan serat kasar LTe sebesar 78,19 %, hemiselulosa 19,22%, neutral detergent fiber 41,69%, acid detergent fiber 61,85%, lignin 64,06%, selulosa 62,47% dan memberikan nilai tertinggi terhadap kecernaan bahan, kecernaan protein, dan kecernaan energi. Hasil uji terhadap kinerja pertumbuhan benih ikan nila merah yang diberi pakan LTe dengan enzim selulase dosis 1,2 g/kg memiliki nilai tertinggi berbeda nyata terhadap pakan kontrol berdasarkan nilai laju pertumbuhan harian (SGR), retensi protein (PR), rasio efisiensi protein (REP) dan perbaikan nilai rasio konversi pakan (RKP). Kata kunci: enzim selulase, ikan nila merah, kecernaan, kinerja pertumbuhan, limbah kecambah kacang hijau
ENHANCING PROTEIN UTILIZATION AND GROWTH PERFORMANCE IN STRIPED CATFISH WITH CINNAMALDEHYDE AND OPTIMIZED ENERGY-TO-PROTEIN RATIOS Wahyudi, Imam Tri; Jusadi, Dedi; Setiawati, Mia; Ekasari, Julie; Suprayudi, Muhammad Agus; Kurniawinata, Mohamad Iqbal; Imlani, Ainulyakin Hasan
Indonesian Aquaculture Journal Vol 20, No 2 (2025): (December, 2025)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.20.2.2025.221-230

Abstract

Efficient dietary protein utilization is essential to reduce feed costs and environmental impacts in sustainable aquaculture. This study aimed to evaluate the effects of cinnamaldehyde (CA) supplementation in feed with various energy-to-protein (E:P) ratios on the chemical composition of Pangasianodon hypophthalmus. The five feed formulas that made up the treatment feed were as follows: 28:13-C0 (28 % Protein with an E:P ratio of 13 and CIN 0 g kg-1); 25:14-C1 (25 % protein with an E:P ratio of 14 and CIN 1.2 g kg-1); 25:14-C2 (25 % protein with an E:P ratio of 14 and CIN 1.7 g kg-1); 25:15-C1 (25 % protein with an E:P ratio of 15 and CIN 1.2 g kg-1); and 25:15-C2 (25 % Protein with an E:P ratio of 15 and CIN 1.7 g kg-1). Striped catfish weighing 28.06 ± 0.19 g were placed in a hapa (2 × 1 × 1 m3) at a density of 25 fish per cage. Fish were fed to apparent satiation three times daily for 60 d. The 25:14-C2, 25:15-C1, and 25:15-C2 diets increased albumin levels and reduced cholesterol, while 25:15-C2 also yielded the highest total protein and lowest triglyceride levels. Growth performance and feed efficiency were comparable among 28:13-C0, 25:14-C2, 25:15-C1, and 25:15-C2 (final weight: 141.62–143.75 g; FCR: 1.16–1.19). Protein efficiency ratio was highest in 25:15-C1 and 25:15-C2, whereas protein retention peaked in 25:14-C2. The hepatosomatic index was elevated in 25:14-C1, 25:14-C2, and 25:15-C1. Body lipid content was highest in 25:15-C1, while muscle lipid content was lowest in 25:14-C2 and 25:15-C1. Reducing dietary protein from 28 % to 25 % did not compromise growth performance at the E:P ratio level of 15 with a supplementation of 1.2 g kg-1 CIN.
BROMELIN PADA PAKAN DAPAT MEMPERBAIKI KINERJA PERTUMBUHAN LARVA IKAN NILA Oreochromis niloticus Rahma, Balqis Aulia; Jusadi, Dedi; Setiawati, Mia; Adestia, Talita Shofa
Media Akuakultur Vol 20, No 2 (2025): Desember 2025
Publisher : Politeknik Kelautan dan Perikanan Bone

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/ma.20.2.2025.63-69

Abstract

Penelitian ini adalah bertujuan untuk mengkaji efek penambahan enzim bromelin dalam pakan terhadap pertumbuhan ikan nila (Oreochromis niloticus) selama tahap pembenihan. Penelitian ini menggunakanRancangan Acak Lengkap (RAL) dengan empat perlakuan pakan yang mengandung bromelin pada kadar 0, 5, 10, serta 15 g kg-1 pakan dan masing-masing diulang sebanyak empat kali. Seratus larva ikan nila, bobotawal rata-rata 0,06±0,02 (g), umur 7 hari ditebar perakuarium dengan volume air 90 L, lalu dipelihara selama 40 hari. Pemberian pakan dilakukan lima kali sehari hingga ikan mencapai tingkat kenyang (at satiation) selama masa pemeliharaan 20 hari pertama dan 4 kali sehari pada 20 hari berikutnya. Hasil penelitian ini membuktikan bahwa penambahan bromelin pada pakan dapat meningkatkan efisiensi pemanfaatan pakan oleh ikan nila karena dapat meningkatkan biomassa ikan (280,60±2,31), rasio efisiensi protein (2,22±0,02), serta menurunkan rasio konversi pakan (1,12±0,01). Bobot individu ikan tidak berbeda nyata antar perlakuan, namun nilai kelangsungan hidup yang tinggi pada perlakuan penambahan bromelin 10 g kg-1, menyebabkan biomassa ikan panen meningkat secara signifikan yang berbeda nyata dari perlakuan 0 g kg-1. Dengan demikian dapat disimpulkan bahwa dosis terbaik penambahan bromelin di fase pembenihan ikan nila adalah 10 g kg-1. 
The Growth Performance of Tilapia (Oreochromis sp.) using Different Doses of Organic Cooper (Cu–O) in the Diets Jusadi, Dedi; Rahmayanti, Yessy; Gumelar, Muhammad Raditya; Adestia, Talita Shofa; Suprayudi, Muhammad Agus
Journal of Aquaculture and Fish Health Vol. 15 No. 1 (2026): JAFH Vol. 15 No. 1 February 2026
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v15i1.78070

Abstract

Copper (Cu-O) is a micromineral required in small amounts in the body, yet it plays an important role in the metabolism and health of fish. This study aimed to evaluate the growth performance of tilapia (Oreochromis sp.) fed diets supplemented with organic copper (Cu-O). Four treatments were tested: K (control, without Cu-O), Cu-O 1 (1 mg kg⁻¹ Cu-O), Cu-O 2 (2 mg kg⁻¹ Cu-O), and Cu-O 3 (3 mg kg⁻¹ Cu-O). Fifteen tilapia with an average initial weight of 8.75 ± 0.02 g were reared in aquaria (60 × 50 × 40 cm, water depth 30 cm) for 60 days. Fish were fed to satiation three times daily. The results showed that the growth parameters of tilapia fed Cu-O-supplemented diets were significantly higher (P < 0.05) than those of the control. The optimal dose of Cu-O supplementation was found to be 2 mg kg⁻¹ feed; subsequent studies should investigate interspecies variations and refine dosage recommendations accordingly.
Co-Authors , Sarmin , Sofian, , . Syafiuddin A. Shofy Mubarak A.I. Nirwana Achmad Noerkhaerin Putra Adang Saputra Ade Sunarma Adiasmara Giri, I Nyoman Agustinus Ngaddi Ahmad Ghufron Mustofa Ahmad Yazid Latif Aimma, Muhammad Ariful Alimuddin Aliyah Sakinah, Aliyah Andi Tiara Eka Diana Puteri, Andi Tiara Eka Diana Anny Hary Ayu Apriana Vinasyiam Arbajayanti, Rahma Dini Asda Laining Asda Laining Audia Wuryas Pradita Negara, Grenda Ayi Rahmat Azis Kurniansyah B.A. Hasyim Dadang Syafruddin Darina Putri Darsiani Darsiani darsiani, Darsiani Deddy Yaniharto Deddy Yaniharto dedy yaniharto Dewi Sulasingkin Diamahesa, Wastu Ayu Didi Humaedi Yusuf, Didi Humaedi Dinamella Wahjuningrum Dini Harianto Dodi Hermawan E. Gandara Eddy Supriyono Enang Harris Enang Harris Enang Harris Enang Harris Enang Harris Enang Harris Endang Purnama Sari Fardila Putri, Rizqiyatul Febrina Rolin Fitriani, Farida Gumelar, Muhammad Raditya Hasan Abidin Hasan Nasrullah Hendriana, Andri Hestirianoto, Totok I Made Artika I MADE ARTIKA I Nyoman Adi Asmara Giri I. Mokoginta Ichsan Achmad Fauzi Ika Wahyuni Putri Imlani, Ainulyakin Hasan Imron Imron, Imron Ing Mokoginta Ing Mokoginta Ing Mokoginta Ing Mokoginta ING MOKOGINTA Ing Mokoginta Iqbal Kurniawinata, Mohamad Irzal Effendi Ismail Rahmat Ismarica, Ismarica Jefry Jefry Jr., Muhammad Zairin Juli Ekasari Julie Ekasari Jullie Ekasari Ketut Sugama Ketut Sugama Kukuh Nirmala M. Zairin Junior Mas Bayu Syamsunarno Mas Tri Djoko Sunarno Mas Tri Djoko Sunarno Mas Tri Djoko Sunarno, Mas Tri Djoko Meilisza, Nina Mia Setiawati Mohamad Amin MUHAMMAD AGUS SUPRAYUDI Muhammad Roikhan Amanullah Muhammad Zairin Jr. Muhammad Zairin Jr. Muhammad Zairin Jr. Mujizat Kawaroe MUNTI YUHANA Nadisa Theresia Putri Neltje Nobertine Palinggi Neltje Nobertine Palinggi nFN Safratilofa Nina Meilisza Nur Bambang Priyo Utomo Nur Bambang Priyo Utomo Nur Bambang Priyo Utomo Nur Hikma Mahasu Nur, Abidin NurBambang Priyono Utomo Nurina Pratiwi Odang Carman Otie Dylan Soebhakti Hasan Pangentasari, Dwinda R Rifai Rahma, Balqis Aulia Rahmayanti, Yessy Rakhmawati, Rakhmawati, Rasidi Rasidi Rasidi, Rasidi Reza Samsudin Ria Septy Anggraini, Ria Septy Ricky Ramadhan RIDWAN AFFANDI Rina Hirnawati Riska Diana Rizkan Fahmi Robin, , Shella Marlinda Shidik, Taufik Shidik Adi Nugroho Siti Komariyah Siti Murniasih Sri Nuryati Sri Nuryati Suardi Laheng Sumantri, Iwan Sumiana, I Kadek Syarifah Ruchyani Syefti Palmi, Revita T.L. Pelawi Talita Shofa Adestia Thoy Batun Citra Rahmadani Tira Silvianti Titin Kurniasih Toshiro Masumoto Triana Retno Palupi Tulas Aprilia Ucu Cahyadi Usman Usman Usman Usman Usman Usman Uttari Dewi Wahyu Pamungkas Wahyu Pamungkas Wahyudi, Imam Tri Wasjan Wasjan Wasjan WIDANARNI WIDANARNI Widya Puspitasari Wijianti, Hani Wiwik Hildayanti Yulfiperius, Yulfiperius Yulintine Yulintine YULISMAN Yuni Puji Hastuti Yutaka Haga Zuraida .