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DEDI JUSADI
Department Of Aquaculture, Faculty Of Fisheries And Marine Science, IPB University, Bogor, West Java 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Library & Information Science Veterinary

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Dietary evaluation of cinnamaldehyde supplementation with different protein energy levels and ratios in Pacific whitleg Shrimp Litopenaeus vannamei Hendriana, Andri; Setiawati, Mia; Jusadi, Dedi; Suprayudi, Muhammad Agus; Ekasari, Julie; Wahjuningrum, Dinamella
Jurnal Akuakultur Indonesia Vol. 23 No. 1 (2024): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.23.1.79-91

Abstract

This study aimed to evaluate the growth performance and carbohydrate metabolism of Pacific whiteleg shrimp after feeding with different cinnamaldehyde concentrations and protein-energy ratios. The study used a completely randomized design with six treatments in triplicates. The treatments were S003213; treatment S053213; treatment S052814; treatment S102814; treatment S052815; and treatment S102815. The study was conducted for 56 days in a 76 L volume aquarium using shrimps with 1.38 ± 0.01 g at 200 individuals/m3. The results showed that the S053213 treatment was significantly different (P<0.05) compared to other treatments for the specific growth rate (SGR). Hexokinase (hk) and phosphoenolpyruvate carboxykinase (pepck) produced by the S053213 treatment were significantly different (P<0.05) from the S003213 treatment. The S052815 and S102815 treatments produced higher protein retention (PR) and protein efficiency ratio (PER) compared to other treatments (P<0.05) and also produced the same final average weight (FW) as the S003213 treatment. This research shows that vannamei fed by 0.10% supplementation dose of cinnamaldehyde with a decreased feed protein up to 28% and C/P ratio 14 and 15 are able to utilize feed as well as protein 32% without cinnamaldehyde. The addition of cinnamaldehyde with a higher C/P ratio requires a higher dose of cinnamaldehyde than the optimal dose. Keywords: Carbohydrate metabolism, cinnamaldehyde, growth, feed energy ratio, Pacific whiteleg shrimp ABSTRAK Penelitian ini bertujuan untuk mengevaluasi kinerja pertumbuhan dan metabolisme karbohidrat udang vaname Litopenaeus vannamei yang diberikan kadar sinamaldehid pada protein dan rasio energi pakan berbeda. Penelitian menggunakan rancangan acak lengkap (RAL) dengan enam perlakuan dan tiga ulangan. Adapun perlakuan terdiri dari perlakuan kontrol S003213; perlakuan S053213; perlakuan S052814; perlakuan S102814; perlakuan S052815; dan perlakuan S102815. Penelitian dilakukan selama 56 hari pemeliharaan pada akuarium volume 76 L menggunakan udang vaname berukuran 1,38 ± 0,01 g dengan kepadatan 200 individuals/m3. Hasil penelitian menunjukkan bahwa perlakuan S053213 berbeda nyata (P<0,05) dibandingkan perlakuan lainnya untuk parameter laju pertumbuhan spesifik (SGR). Parameter heksokinase (hk) dan phosphoenolpyruvate carboxykinase (pepck) yang tertinggi dihasilkan oleh perlakuan S053213 dan berbeda nyata (P<0,05) dengan perlakuan S003213. Pada perlakuan S052815 dan S102815 menghasilkan retensi protein (PR) dan rasio efisiensi protein (PER) lebih tinggi dibandingkan dengan perlakuan lainnya (P<0,05) serta menghasilkan bobot rata-rata akhir (FW) sama dengan perlakuan S003213. Penelitian ini menunjukkan bahwa udang vaname yang diberikan suplementasi sinamaldehid sebesar 0,10% dengan protein pakan 28% dan rasio C/P menjadi 14 dan 15 mampu pemanfaatan protein pakan yang sama dengan protein pakan 32% tanpa suplementasi sinamaldehid. Penambahan sinamaldehid dengan rasio C/P yang lebih tinggi membutuhkan dosis sinamaldehid yang lebih tinggi dari dosis optimal. Kata kunci: metabolisme karbohidrat, pertumbuhan, rasio energi pakan, sinamaldehid, udang vaname
Utilization of fish bone charcoal in feed on growth and physiological responses of catfish fry Clarias gariepinus Fardila Putri, Rizqiyatul; Setiawati, Mia; Jusadi, Dedi; Ekasari, Julie
Jurnal Akuakultur Indonesia Vol. 24 No. 1 (2025): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.24.1.82-91

Abstract

This study aimed to assess the impact of charcoal inclusion in feed on the physiological responses and growth of catfish. The experiment employed a completely randomized design (CRD) with five treatments and four replicates. Charcoal doses in the feed were set at 0% (C0, control), 0.5% (C0.5), 1% (C1), 2% (C2), and 3% (C3). The catfish used weighed 3.95 ± 0.03 g and were kept in 20 hapa units (2×1×1 m³) with a stocking density of 70 fish/hapa for 60 days. The results indicated significant differences (p<0.05) in the hepatosomatic index between treatments, with the highest values recorded in C1 (2.15 ± 0.10b) and C0.5 (1.91 ± 0.19b). However, liver glycogen levels did not significantly vary across treatments (p>0.05). Charcoal supplementation enhanced blood mineral levels (calcium, phosphorus, manganese, and zinc) in the C2 and C3 groups. The highest blood glucose level was observed in C1 (p<0.05). Intestinal histological analysis showed that the highest villi height and surface area were recorded in the C2 group, with values of 540.0 ± 10.2 µm and 34.122 ± 1.311 µm², respectively. Additionally, 2% charcoal supplementation improved final weight, daily growth rate, and protein retention, while reducing feed consumption and the feed conversion ratio compared to the control. Overall, the inclusion of 2% charcoal positively influenced the intestinal histology of catfish, contributing to enhanced growth performance. The study also demonstrated that charcoal addition affected the hepatosomatic index, blood glucose, and blood mineral levels in catfish. Keywords: catfish, charcoal, growth, physiological response ABSTRAK Penelitian ini bertujuan untuk mengevaluasi pengaruh pemberian charcoal dalam pakan terhadap respons fisiologis dan pertumbuhan ikan lele. Penelitian dirancang dengan Rancangan Acak Lengkap (RAL) dengan lima perlakuan dan empat ulangan. Perlakuan dosis charcoal yang yang berbeda yaitu 0 (C0, kontrol), 0.5 (C0,5), 1 (C1), 2 (C2), dan 3% (C3). Ikan lele yang digunakan berbobot 3,95 ± 0,03 g/ekor dipelihara di hapa berukuran 2×1×1 m³ sebanyak 20 unit dengan kepadatan 70 ekor/hapa selama 60 hari. Hasil penelitian menunjukkan hepatosomatik indeks beda nyata antar perlakuan (p<0,05) nilai tertinggi terdapat pada perlakuan C1 2,15 ± 0,10b dan C0,5 1,91± 0,19b. Penambahan charcoal pada pakan tidak berbeda nyata terhadap glikogen hati (P>0,05). Penambahan charcoal dalam pakan dapat meningkatkan kandungan mineral darah ikan (kalsium, fosfor, mangan, dan zinc) pada perlakuan C2 dan C3. Nilai glukosa darah tertinggi pada pakan yang diberi pakan C1 (p<0,05). Hasil histologi usus menunjukkan nilai tertinggi pada tinggi vili dan luas permukaan vili usus terdapat pada perlakuan C2 (2% charcoal) dengan tinggi vili (540,0±10,2 µm) dan luas permukaan vili (34122 ± 1311 µm). Penambahan charcoal 2% pada pakan dapat meningkatkan bobot akhir, laju pertumbuhan harian, retensi protein, menurunkan konsumsi pakan dan feed convertion ratio dibandingkan kontrol. Pemberian charcoal 2% dalam pakan berpengaruh baik terhadap histologi usus ikan lele, sehingga dapat meningkatkan pertumbuhan pada perlakuan C2. Pada penelitian ini juga di temukan bahwa penambahan charcoal dalam pakan berpengaruh terhadap indeks hepatosomatik, glukosa darah, serta mineral darah ikan lele. Kata kunci : charcoal, ikan lele, pertumbuhan, respons fisiologis
Rotifer Brachionus rotundiformis enriched with taurine for survival and growth of larval milkfish Chanos chanos Aimma, Muhammad Ariful; Dedi Jusadi; Suprayudi, Muhammad Agus; Alimuddin, Alimuddin; Adiasmara Giri, I Nyoman; Shofa Adestia, Talita
Jurnal Akuakultur Indonesia Vol. 24 No. 1 (2025): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.24.1.102-110

Abstract

This research evaluated the effect of taurine-enriched rotifers on the survival and growth of milkfish larvae. Twenty-five fertilized milkfish eggs were placed in a 250 L cylindrical fiber tank. This study used a completely randomized design with four treatments and five replications. Three days old larvae were given rotifers enriched with taurine at 0, 25, 50, or 75 mg/L. Larvae were cultured until they were 18 days old. The results showed that the taurine content in rotifers was higher in rotifer at 50 mg/L treatment, then decreased at the 75 mg/L treatment. Taurine content in the milkfish larvae aligned with taurine levels in rotifers. Furthermore, the taurine content in rotifers affected milkfish larvae' survival and body length. The highest larval survival and growth was obtained in the 50 mg/L taurine treatment. Thus, it can be concluded that taurine at 50 mg/L is the optimal rotifer enrichment dose for milkfish larvae. Keywords: growth, milkfish larvae, notochord, survival rate, taurine ABSTRAK Penelitian ini dilakukan untuk mengevaluasi pengaruh pemberian rotifera yang diperkaya taurin terhadap kelangsungan hidup dan pertumbuhan larva ikan bandeng. Dua puluh lima butir telur ikan bandeng yang telah dibuahi ditempatkan pada tangki fiber berbentuk silinder berukuran 250 L. Penelitian ini menggunakan rancangan acak lengkap dengan empat perlakuan dan lima ulangan. Larva berumur tiga hari setelah menetas diberi rotifer yang diperkaya taurin dengan dosis 0, 25, 50, atau 75 mg/L. Larva dipelihara hingga berumur 18 hari. Hasil penelitian menunjukkan bahwa kandungan taurin pada rotifer tertinggi diperoleh pada perlakuan rotifer 50 mg/L, kemudian menurun pada perlakuan rotifer 75 mg/L. Kandungan taurin pada larva ikan bandeng sebanding dengan kandungan taurin pada rotifer. Kandungan taurin pada rotifer berpengaruh nyata terhadap kelangsungan hidup dan panjang tubuh larva ikan bandeng. Kelangsungan hidup dan pertumbuhan larva tertinggi diperoleh pada perlakuan dosis 50 mg/L. Dari hasil penelitian dapat disimpulkan bahwa pemberian taurin 50 mg/L pada pengayaan rotifer merupakan dosis yang optimal untuk diberikan pada larva ikan bandeng. Kata Kunci: kelangsungan hidup, larva bandeng, notochord, pertumbuhan, taurin
The dynamics glucose on carbohydrate utilization of striped catfish Pangasianodon hypophthalmus with yacon leaf Smallanthus sonchifolius dietary supplementation Syefti Palmi, Revita; Setiawati, Mia; Jusadi, Dedi; Ekasari, Julie
Jurnal Akuakultur Indonesia Vol. 24 No. 2 (2025): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.24.2.158-171

Abstract

The objective of the study was to test dietary supplementation of yacon leaf on the carbohydrate utilization, growth performance, and glucose tolerance of striped catfish during the early-stage period. Fish were fed with five different levels of yacon leaf supplementation (0%, 0.5%, 1%, 1.5%, 2%) for nine weeks. Three hundred striped catfish (initial mean body weight: 14 ± 0.14 g) were maintained in 15 rectangular net cages. The results showed that the specific growth rate of fish with yacon leaf supplementation (3.46-3.79%) was higher than the control fish (3.19%) also the feed efficiency value. The highest protein retention was found on 1% yacon leaf fish diet, while the highest lipid retention was found on 1.5%. The blood glucose post-prandial was rise slower than the control fish. The glucose tolerance test also obtained less time to return to basal level after the glucose load. The increased of enzymatic were also present at the supplemented fish. The liver lipid and glycogen concentration was decreased, and the hepatic somatic index was increased. The blood biochemistry showed the lower level of total protein plasma and albumin in supplemented fish, and higher triglyceride and cholesterol levels due to the replenishment of energy storage in adipose tissues while fasting. Therefore, this study concludes that the yacon leaf dietary supplementation has the potential to improve carbohydrate utilization by promoting glucose use efficiently as the main energy, while protein can be utilized for growth. Our data suggest that the best level of yacon leaf supplementation in fish diets is 1.36%. Keywords: yacon-leaf, homeostatic, glucose, sparing-effect ABSTRAK Tujuan dari penelitian ini adalah untuk mengkaji efektifitas suplementasi daun insulin pada pakan terhadap pemanfaatan karbohidrat, kinerja pertumbuhan, dan toleransi glukosa pada benih ikan patin. Ikan diberi pakan dengan lima tingkat suplementasi daun insulin yang berbeda yaitu 0%, 0,5%, 1%, 1,5%, 2% selama sembilan minggu. Tiga ratus benih ikan patin (rata-rata biomasa awal: 14 ± 0,14 g) dipelihara dalam 15 keramba jaring berbentuk persegi panjang. Hasil penelitian menunjukkan bahwa laju pertumbuhan spesifik ikan yang diberi suplementasi daun insulin (3,46-3,79%) lebih tinggi dibandingkan ikan kontrol (3,19%) linier dengan nilai efisiensi pakan. Retensi protein tertinggi terdapat pada ikan dengan pakan daun insulin 1%, sedangkan retensi lemak tertinggi terdapat pada pakan 1,5%. Kenaikan glukosa darah post-prandial lebih lambat dibandingkan pada ikan kontrol. Kadar toleransi glukosa juga memperoleh waktu yang lebih singkat untuk kembali ke level basal setelah injeksi glukosa. Peningkatan aktivitas enzimatik juga terjadi pada ikan yang diberi suplementasi daun insulin. Konsentrasi lipid dan glikogen hati menurun, dan indeks somatik hati meningkat. Biokimia darah menunjukkan rendahnya kadar total protein plasma dan albumin pada ikan yang diberi suplementasi, serta kadar trigliserida dan kolesterol yang lebih tinggi selama proses pemulihan menggunakan cadangan energi dari jaringan adiposa saat dipuasakan. Oleh karena itu, penelitian ini menyimpulkan bahwa suplementasi daun insulin berpotensi untuk meningkatkan pemanfaatan karbohidrat dengan menstimulasi penggunaan glukosa secara efisien sebagai energi utama, sementara protein dapat dimanfaatkan untuk pertumbuhan. Data kami menunjukkan bahwa tingkat suplementasi daun insulin terbaik dalam pakan ikan adalah 1,36%. Kata kunci: daun insulin, efek hemat, homeostatis, glukosa
RESPONS PERTUMBUHAN, PEMANFAATAN NUTRIEN, DAN AKTIVITAS ANTIOKSIDAN IKAN TORSORO (Tor soro) YANG DIBERI PAKAN DENGAN SUPLEMENTASI GLUTAMIN Samsudin, Reza; Jusadi, Dedi; Setiawati, Mia; Widanarni, Widanarni; Alimuddin, Alimuddin; Sunarno, Mas Tri Djoko
Jurnal Riset Akuakultur Vol 19, No 3 (2024): September (2024)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.19.3.2024.205-227

Abstract

Penelitian ini bertujuan untuk mengevaluasi pengaruh suplementasi glutamin terhadap pertumbuhan dan aktivitas antioksidan pada ikan Torsoro (Tor soro). Ikan uji yang digunakan adalah juvenil ikan dengan berat awal rata-rata 5,21 ± 0,04 g. Perlakuan yang diberikan terdiri atas suplementasi glutamin bebas dengan dosis yang berbeda (0; 0,5; 1,0; 1,5; dan 2%) dan alanil-glutamin (0,84; 1,67; 2,51; dan 3,35%) pada pakan, setiap perlakuan memiliki lima kali ulangan. Perlakuan pakan diberikan tiga kali sehari sekenyangnya. Ikan uji dipelihara selama 60 hari. Parameter yang diamati meliputi performa pertumbuhan, pemanfaatan nutrisi, indeks biologis, dan aktivitas antioksidan. Hasil penelitian menunjukkan bahwa pakan yang disuplementasi glutamin bebas berpengaruh nyata (p<0,05) terhadap performa pertumbuhan, pemanfaatan nutrisi, indeks biologis, dan aktivitas antioksidan ikan uji. Hasil uji lebih lanjut menunjukkan bahwa suplementasi alanil-glutamin dalam pakan sebesar 2,51% memberikan hasil terbaik terhadap bobot akhir, laju pertumbuhan spesifik, retensi protein, rasio efisiensi protein, konversi pakan, rasio viseral somatik, rasio panjang usus, aktivitas superoksida dismutase, glutation peroksidase, dan katalase pada ikan Torsoro. Berdasarkan evaluasi keseluruhan terhadap parameter yang diamati, penelitian ini menunjukkan bahwa suplementasi alanil-glutamin dalam pakan pada tingkat 2,51% mampu meningkatkan kinerja pertumbuhan, pemanfaatan nutrisi, indeks biologis, dan aktivitas antioksidan pada ikan Torsoro.This study aimed to evaluate the effects of glutamine supplementation on the growth of and antioxidant activity in Torsoro fish (Tor soro). The test fish used were Torsoro fish juveniles with an average initial weight of 5.21 ± 0.04 g. The treatments consisted of different supplementation levels of free glutamine (0; 0.5; 1.0; 1.5; and 2%) and alanyl-glutamine (0.84; 1.67; 2.51; and 3.35%) in feed, where each treatment had five replicates. The feed treatments were given three times a day ad satiation. The experimental fish were reared for 60 days. The parameters observed included growth performance, nutrient utilization, biological indices, and antioxidant activity. The results showed that free glutamine-supplemented feed significantly affects (p<0.05) the growth performance, nutrient utilization, biological indices, and antioxidant activity of the tested fish. Further test results showed that alanyl glutamine supplementation in feed at 2.51% produced the best results on the final weight, specific growth rate, protein retention, protein efficiency ratio, feed conversion, somatic visceral ratio, intestine length ratio, superoxide dismutase, glutathione peroxidase, and catalase activities of Torsoro fish. Based on the overall achievement of the observed parameters, this study determines that alanyl-glutamine supplementation in feed at 2.51% improves the growth performance, nutrient utilization, biological indices, and antioxidant activity of  Torsoro fish.
SUPLEMENTASI L-KARNITIN DAN KAYU MANIS PADA PAKAN TERHADAP PENURUNAN LEMAK DAN TEKSTUR FILET IKAN PATIN Pangasianodon hypophthalmus PADA FASE PEMBESARAN Wahyudi, Imam Tri; Jusadi, Dedi; Setiawati, Mia; Ekasari, Julie; Suprayudi, Muhammad Agus
Jurnal Riset Akuakultur Vol 18, No 1 (2023): (Maret 2023)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.18.1.2023.1-14

Abstract

upaya untuk mengurangi kadar lemak tersebut. Tujuan penelitian ini adalah mengevaluasi suplementasi L-karnitin dan tepung kayu manis terhadap kadar lemak dan tekstur daging ikan patin pada fase pembesaran. Ikan patin berukuran 125,4 ± 7,85 g dan panjang 24,71±0,68 cm dipelihara selama 60 hari dalam wadah hapa berukuran 2 x 1 x 1 m3. Ikan diberi pakan tiga kali sehari ad satiation dengan perlakuan, sebagai berikut: kontrol (K), penambahan L-karnitin 1 g kg-1 (LK 1), L-karnitin 2 g kg-1 (LK 2), kayu manis 5 g kg-1 (KM 5), dan kayu manis 10 g kg-1 (KM 10). Sampel diambil pada awal, pertengahan, dan akhir pemeliharaan untuk pengukuran kadar lemak daging. Parameter yang diamati yaitu lemak daging dan tekstur daging. Hasil menunjukkan bahwa pemberian KM 10 menghasilkan kadar lemak yang lebih rendah dari perlakuan lainnya setelah 30 hari dan penurunan yang lebih besar yaitu berkisar 51,06% dan 42,55% pada perlakuan LK 2 dan KM 10 pada hari ke-60. Nilai indeks hepatosomatik juga menurun yang diikuti oleh penurunan kadar lemak hati. Nilai kekerasan daging menunjukkan peningkatan kualitas yang terlihat dari nilai yang semakin rendah. Pemberian tepung kayu manis 10 g kg-1 pada fase pembesaran menunjukkan hasil terbaik pada pemberian selama 60 hari dalam menurunkan lemak daging ikan patin hingga memenuhi standar filet, karena adanya proses lipolisis serta pemanfaatan lemak menjadi energi. Kayu manis ini menjadi sangat potensial untuk dikembangkan sebagai feed additive dalam upaya memperbaiki kualitas daging ikan patin fase pembesaran.Striped catfish meat has a high-fat content which is undesirable to markets and customers alike and needs to be reduced. This study aims to evaluate the effect of dietary L-carnitine and cinnamon powder on reducing the fat content of striped catfish meat in the grow-out stage. Striped catfish measuring 125.4 ± 7.85 g and 24.71 ± 0.68 cm body length were kept for 60 days in a hapa with size 2 x 1 x 1 m3. Thefish were fed three times a day at satiation with the following treatments: control (K), the addition of L-carnitine 1 g kg-1 (LK 1), L-carnitine 2 g kg-1 (LK 2), cinnamon 5 g kg-1 (KM 5) and cinnamon 10 g kg-1 (KM 10). Sampling was conducted on the initial, middle and final day for meat fat content analysis. The parameters observed were meat fat and meat texture. The results showed that dietary KM 10 significantly reduced meat fat content compared to controls on the 30th and it showed a more significant reduction, namely 51.06% and 42.55%, in the treatment LK 2 and KM 10 after 60 days treatment. The hepatosomatic index value also decreased, followed by decreased liver fat levels. The lower values of meat hardness imply an increase in meat quality. It can be concluded that the application of dietary cinnamon powder at 10 g kg-1 (KM 10) is the best level to reduce the fat content of striped catfish meat in meeting the fillet standards. This cinnamon has excellent potential to be developed as a feed additive to improve the quality of striped catfish meat in the rearing phase.
ENHANCING PROTEIN UTILIZATION AND GROWTH PERFORMANCE IN STRIPED CATFISH WITH CINNAMALDEHYDE AND OPTIMIZED ENERGY-TO-PROTEIN RATIOS Wahyudi, Imam Tri; Jusadi, Dedi; Setiawati, Mia; Ekasari, Julie; Suprayudi, Muhammad Agus; Kurniawinata, Mohamad Iqbal; Imlani, Ainulyakin Hasan
Indonesian Aquaculture Journal Vol 20, No 2 (2025): (December, 2025)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.20.2.2025.221-230

Abstract

Efficient dietary protein utilization is essential to reduce feed costs and environmental impacts in sustainable aquaculture. This study aimed to evaluate the effects of cinnamaldehyde (CA) supplementation in feed with various energy-to-protein (E:P) ratios on the chemical composition of Pangasianodon hypophthalmus. The five feed formulas that made up the treatment feed were as follows: 28:13-C0 (28 % Protein with an E:P ratio of 13 and CIN 0 g kg-1); 25:14-C1 (25 % protein with an E:P ratio of 14 and CIN 1.2 g kg-1); 25:14-C2 (25 % protein with an E:P ratio of 14 and CIN 1.7 g kg-1); 25:15-C1 (25 % protein with an E:P ratio of 15 and CIN 1.2 g kg-1); and 25:15-C2 (25 % Protein with an E:P ratio of 15 and CIN 1.7 g kg-1). Striped catfish weighing 28.06 ± 0.19 g were placed in a hapa (2 × 1 × 1 m3) at a density of 25 fish per cage. Fish were fed to apparent satiation three times daily for 60 d. The 25:14-C2, 25:15-C1, and 25:15-C2 diets increased albumin levels and reduced cholesterol, while 25:15-C2 also yielded the highest total protein and lowest triglyceride levels. Growth performance and feed efficiency were comparable among 28:13-C0, 25:14-C2, 25:15-C1, and 25:15-C2 (final weight: 141.62–143.75 g; FCR: 1.16–1.19). Protein efficiency ratio was highest in 25:15-C1 and 25:15-C2, whereas protein retention peaked in 25:14-C2. The hepatosomatic index was elevated in 25:14-C1, 25:14-C2, and 25:15-C1. Body lipid content was highest in 25:15-C1, while muscle lipid content was lowest in 25:14-C2 and 25:15-C1. Reducing dietary protein from 28 % to 25 % did not compromise growth performance at the E:P ratio level of 15 with a supplementation of 1.2 g kg-1 CIN.
BROMELIN PADA PAKAN DAPAT MEMPERBAIKI KINERJA PERTUMBUHAN LARVA IKAN NILA Oreochromis niloticus Rahma, Balqis Aulia; Jusadi, Dedi; Setiawati, Mia; Adestia, Talita Shofa
Media Akuakultur Vol 20, No 2 (2025): Desember 2025
Publisher : Politeknik Kelautan dan Perikanan Bone

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/ma.20.2.2025.63-69

Abstract

Penelitian ini adalah bertujuan untuk mengkaji efek penambahan enzim bromelin dalam pakan terhadap pertumbuhan ikan nila (Oreochromis niloticus) selama tahap pembenihan. Penelitian ini menggunakanRancangan Acak Lengkap (RAL) dengan empat perlakuan pakan yang mengandung bromelin pada kadar 0, 5, 10, serta 15 g kg-1 pakan dan masing-masing diulang sebanyak empat kali. Seratus larva ikan nila, bobotawal rata-rata 0,06±0,02 (g), umur 7 hari ditebar perakuarium dengan volume air 90 L, lalu dipelihara selama 40 hari. Pemberian pakan dilakukan lima kali sehari hingga ikan mencapai tingkat kenyang (at satiation) selama masa pemeliharaan 20 hari pertama dan 4 kali sehari pada 20 hari berikutnya. Hasil penelitian ini membuktikan bahwa penambahan bromelin pada pakan dapat meningkatkan efisiensi pemanfaatan pakan oleh ikan nila karena dapat meningkatkan biomassa ikan (280,60±2,31), rasio efisiensi protein (2,22±0,02), serta menurunkan rasio konversi pakan (1,12±0,01). Bobot individu ikan tidak berbeda nyata antar perlakuan, namun nilai kelangsungan hidup yang tinggi pada perlakuan penambahan bromelin 10 g kg-1, menyebabkan biomassa ikan panen meningkat secara signifikan yang berbeda nyata dari perlakuan 0 g kg-1. Dengan demikian dapat disimpulkan bahwa dosis terbaik penambahan bromelin di fase pembenihan ikan nila adalah 10 g kg-1. 
Utilization of mung bean sprout waste Vigna radiata hydrolyzed cellulase enzyme in feed on the digestibility of Nile tilapia Oreochromis sp. Grenda Audia Wuryas Pradita Negara; Mia Setiawati; Julie Ekasari; Dedi Jusadi
Jurnal Akuakultur Indonesia Vol. 25 No. 1 (2026): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.25.1.138-150

Abstract

This study evaluated the utilization of mung bean sprout waste flour hydrolyzed by cellulase enzyme (LTe) as the feed ingredient of red Nile tilapia weighing 10.00 ± 0.01 g/seed and 7.00 ± 0.15 in length. This study used two stages, each consisting of four treatments and four replications. The first step was performed by evaluating LTe flour added with cellulase enzyme of 0 g/kg (control), 0.4 g/kg, 0.8 g/kg, and 1.2 g/kg. The second step was the digestibility test of LTe and growth performance on red Nile tilapia seeds. The results showed that the addition of cellulase enzyme at a 1.2 g/kg was significantly able to reduce the crude fiber of LTe with 78.19% %, hemicellulose at 19.22%, neutral detergent fiber at 41.69%, acid detergent fiber at 61.85%, lignin 64.06%, cellulose 62.47% besides having the best value of ingredient, protein, and energy digestibility. The test results on the growth performance of red Nile tilapia seeds fed with LTe feed with a dose of 1.2 g/kg cellulase enzyme have the highest value significantly different from the control feed based on the value of daily growth rate (SGR), ratio efficiency protein (REP), protein retention (PR), and improvement of feed conversion ratio (RKP).   Keywords: cellulase enzyme, digestibility, growth performance, mung bean sprout waste, tilapia   Abstrak Penelitian ini mengevaluasi pemanfaatan tepung limbah kecambah kacang hijau yang dihidrolisis enzim selulase (LTe) sebagai bahan baku pakan pada benih ikan nila merah dengan bobot 10,00 ± 0,01 g/ekor dan panjang 7,00 ± 0,15 cm. Penelitian ini menggunakan dua tahap dan masing-masing tahap terdiri dari empat perlakuan dan empat ulangan. Tahap pertama dilakukan evaluasi tepung LTe sebesar 0 g/kg (kontrol), 0,4 g/kg, 0,8 g/kg, dan 1,2 g/kg. Tahap kedua dilakukan uji kecernaan bahan LTe dan kinerja pertumbuhan benih ikan nila merah. Hasil penelitian menunjukkan bahwa penambahan enzim selulase pada dosis 1,2 g/kg signifikan mampu menurunkan serat kasar LTe sebesar 78,19 %, hemiselulosa 19,22%, neutral detergent fiber 41,69%, acid detergent fiber 61,85%, lignin 64,06%, selulosa 62,47% dan memberikan nilai tertinggi terhadap kecernaan bahan, kecernaan protein, dan kecernaan energi. Hasil uji terhadap kinerja pertumbuhan benih ikan nila merah yang diberi pakan LTe dengan enzim selulase dosis 1,2 g/kg memiliki nilai tertinggi berbeda nyata terhadap pakan kontrol berdasarkan nilai laju pertumbuhan harian (SGR), retensi protein (PR), rasio efisiensi protein (REP) dan perbaikan nilai rasio konversi pakan (RKP). Kata kunci: enzim selulase, ikan nila merah, kecernaan, kinerja pertumbuhan, limbah kecambah kacang hijau
Development of Siganid (Siganus guttatus) larvae during the transition period Darsiani Darsiani; Mia Setiawati; Dedi Jusadi; Muhammad Agus Suprayudi; Asda Laining
Depik Vol 11, No 1 (2022): April 2022
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.11.1.22296

Abstract

Siganid is better known as rabbit fish. In hatcheries, constraint that is still faced is the low survival, which was assumed to occur because of the timing for initial feeding is not solidly known. This research aimed to examine the best initial feeding time for siganus, based on evaluation on eyes and yolk reserves during the transition. The research was conducted from 24-29 March 2021 in IPUW Barru, South Sulawesi. Larvae were obtained from the second progeny (G2) of domesticated Siganus guttatus. Larvae were reared for 5-6 days without feeding. Evaluated parameters include eyes diameter and yolk reserves. Samples were observed with microscope and will be explained descriptively. Water quality parameters were measured, namely DO, salinity, pH and temperature. Eyes diameter at 6 Hour After Hatching (HAH) ranges between 81.5-128.9 m, 13 HAH= 125.5-167.7 m, 24 HAH= 138.2-213.9 m, two days after hatching 2 Day After Hatching (DAH) = 113.6-193.1 m, 3 DAH= 163.1-219.2 m, 4 DAH= 190.4-212.6 m. Yolk reserves diameter ranged between 137-194m (6 HAH), 13 HAH= 152-191m, 24 HAH= 94.0-185m, 2 DAH= 75.3-99.63m, 3 DAH= 42.33-87.58m, 4 DAH= 38.17-55.59m. After age 5 DAH, there are no larvae found alive (dead). Eyes developed at age 6 HAH and experienced pigmentation at age 24 HAH. Conversely, yolk reserves diameters were getting smaller since age 24 HAH and completely disappear at age 4 DAH. It indicates that eyes effectively see feeds at age 2 DAH. Therefore, initial feeding should be started. The water quality parameters measured were still in normal conditions according to the life of S. guttatus larvae. From this research, it can be concluded that eyes have been well functioned at age 2 DAH and yolk reserves was finished at age 4 DAH. Therefore, the initial feeding should be done at the age of 2 DAH.Keywords:Development, Siganus guttatus larvae,Transition
Co-Authors , Sarmin , Sofian, , . Syafiuddin A. Shofy Mubarak A.I. Nirwana Achmad Noerkhaerin Putra Adang Saputra Ade Sunarma Adiasmara Giri, I Nyoman Agustinus Ngaddi Ahmad Ghufron Mustofa Ahmad Yazid Latif Aimma, Muhammad Ariful Alimuddin Aliyah Sakinah, Aliyah Andi Tiara Eka Diana Puteri, Andi Tiara Eka Diana Anny Hary Ayu Apriana Vinasyiam Asda Laining Ayi Rahmat Azis Kurniansyah B.A. Hasyim Dadang Syafruddin Darina Putri Darsiani Darsiani Deddy Yaniharto Deddy Yaniharto dedy yaniharto Dewi Sulasingkin Diamahesa, Wastu Ayu Didi Humaedi Yusuf, Didi Humaedi Dinamella Wahjuningrum Dini Harianto Dodi Hermawan E. Gandara Eddy Supriyono Enang Harris Enang Harris Enang Harris Enang Harris Enang Harris Enang Harris Endang Purnama Sari Fardila Putri, Rizqiyatul Febrina Rolin Fitriani, Farida Grenda Audia Wuryas Pradita Negara Hasan Abidin Hasan Nasrullah Hendriana, Andri Hestirianoto, Totok I Made Artika I MADE ARTIKA I Nyoman Adi Asmara Giri I. Mokoginta Ichsan Achmad Fauzi Ika Wahyuni Putri Imlani, Ainulyakin Hasan Imron Imron, Imron ING MOKOGINTA Ing Mokoginta ING MOKOGINTA Ing Mokoginta Ing Mokoginta Ing Mokoginta Ing Mokoginta Iqbal Kurniawinata, Mohamad Irzal Effendi Ismail Rahmat Ismarica, Ismarica Jefry Jefry Juli Ekasari Julie Ekasari Jullie Ekasari Ketut Sugama Ketut Sugama Kukuh Nirmala M. Zairin Junior Mas Bayu Syamsunarno Mas Tri Djoko Sunarno Mas Tri Djoko Sunarno Mas Tri Djoko Sunarno, Mas Tri Djoko Meilisza, Nina Mia Setiawati Mohamad Amin MUHAMMAD AGUS SUPRAYUDI Muhammad Roikhan Amanullah Muhammad Zairin Jr. Muhammad Zairin Jr. Muhammad Zairin Jr. Muhammad Zairin Jr. Mujizat Kawaroe MUNTI YUHANA Nadisa Theresia Putri Neltje Nobertine Palinggi Neltje Nobertine Palinggi nFN Safratilofa Nina Meilisza Nur Bambang Priyo Utomo Nur Hikma Mahasu Nur, Abidin NurBambang Priyono Utomo Nurina Pratiwi Odang Carman Otie Dylan Soebhakti Hasan Pangentasari, Dwinda R Rifai Rahma Dini Arbajayanti Rahma, Balqis Aulia Rahmadani, Thoy Batun Citra Rakhmawati, Rakhmawati, Rasidi Rasidi Rasidi Rasidi Reza Samsudin Ria Septy Anggraini, Ria Septy Ricky Ramadhan RIDWAN AFFANDI Rina Hirnawati Riska Diana Rizkan Fahmi Robin, , Shella Marlinda Shidik, Taufik Shidik Adi Nugroho Siti Komariyah Siti Murniasih Sri Nuryati Sri Nuryati Suardi Laheng Sumantri, Iwan Sumiana, I Kadek Syarifah Ruchyani Syefti Palmi, Revita T.L. Pelawi Talita Shofa Adestia Tira Silvianti Titin Kurniasih Toshiro Masumoto Triana Retno Palupi Tulas Aprilia Ucu Cahyadi Usman Usman Usman Usman Usman Usman Uttari Dewi Wahyu Pamungkas Wahyu Pamungkas Wahyudi, Imam Tri Wasjan Wasjan Wasjan WIDANARNI WIDANARNI Widya Puspitasari Wijianti, Hani Wiwik Hildayanti Yulfiperius, Yulfiperius Yulintine Yulintine YULISMAN Yuni Puji Hastuti Yutaka Haga Zuraida .