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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Akuakultur Indonesia Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Jurnal Akuakultur Rawa Indonesia ILMU KELAUTAN: Indonesian Journal of Marine Sciences Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology BIOTROPIA - The Southeast Asian Journal of Tropical Biology Omni-Akuatika Indonesian Aquaculture Journal Jurnal Riset Akuakultur Media Akuakultur Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Journal of Aquaculture and Fish Health Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Jurnal Perikanan
DEDI JUSADI
Department Of Aquaculture, Faculty Of Fisheries And Marine Science, IPB University, Bogor, West Java 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Library & Information Science Veterinary

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Journal : Omni-Akuatika

 1 
6-Desaturase-Like Encoding Gene Introduction in Catfish (Clarias gariepinus) Anny Hary Ayu; Alimuddin Alimuddin; Dedi Jusadi
Journal Omni-Akuatika Vol 13, No 2 (2017): Omni-Akuatika November
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (347.456 KB) | DOI: 10.20884/1.oa.2017.13.2.188

Abstract

African catfish (Clarias gariepinus) is one of the economically valuable aquaculture fish species in Indonesia. This research was aimed to produce F0 transgenic catfish carrying masou salmon Δ6-desaturase-like (OmΔ6FAD) gene. The Δ6-desaturase enzyme is involved in highly unsaturated fatty acid biosynthesis. Transgenic catfish was produced by sperm-mediated gene transfer using electroporation method. In this study, as the first step, sperms were electroporated with three different OmΔ6FAD concentration (25, 50, and 100 µg mL-1) to have the highest sperm viability after electroporation (125 V/cm, pulse frequency 5 times, pulse length 30 millisecond, pulse interval 0.1 second). The highest sperm viability and sperm carrying OmΔ6FAD were obtain at 100 µg mL-1. This concentration was then used to produce F0 transgenic catfish in the second step. Sperm motility, sperm viability, fertilization rate, hatching rate, and larval survival at 14 days after hatching were the same as the controls (p>0.05). Genomic DNA was extracted from caudal fin and then used as template to identify transgenic F0 by PCR method using specific primer for OmΔ6FAD gene. The PCR result showed that 53.84% of F0 carried OmΔ6FAD gene. The result of fatty acid analysis showed that EPA and DHA contents of F0 transgenic fish and non-transgenic fish were similar. Keywords: catfish, Δ6-desaturase-like gene, fatty acids, electroporation
Design a Photobioreactor for Microalgae Cultivation with the IOTs (Internet of Things) System Ayi Rahmat; Indra Jaya; Totok Hestirianoto; Dedi Jusadi; Mujizat Kawaroe
Journal Omni-Akuatika Vol 16, No 1 (2020): Omni-Akuatika May
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.oa.2020.16.1.791

Abstract

Internet of Things (IOTs) is an evolution of the concept of internet use that aims to expand the benefits of internet connectivity that is connected continuously with the ability to control remotely (remote control), share data (data sharing), carry out continuous monitoring (real time monitoring) and current (up to date). This research related to the cultivation of microalgae as a source of food and energy of the future, in the design of photobioreactors that are integrated with IoT, so that it can be monitored continuously, controlled and used as a model for the development of greater microalgae cultivation technology. Cultivation in this study was a closed system photobioreactor, will produce microalgae that are not contaminated by external contaminants, growth analysis can be done based on the parameters that influence it, including the cultivation room temperature, lighting level (luminance), and the color of water in the photosynthesis process of microalgae, and also control of water circulation. Visualization of controlled parameters includes, temperature parameters, light intensity, water color changes. The observed parameters will be displayed in a graphical user interface (GUI) in real time using the internet. The advantages of this system can see the growth of microalgae in detail over time, and obtained raw data that can be processed for various research purposes.
Co-Authors , Sarmin , Sofian, , . Syafiuddin A. Shofy Mubarak A.I. Nirwana Achmad Noerkhaerin Putra Adang Saputra Ade Sunarma Adiasmara Giri, I Nyoman Agustinus Ngaddi Ahmad Ghufron Mustofa Ahmad Yazid Latif Aimma, Muhammad Ariful Alimuddin Aliyah Sakinah, Aliyah Andi Tiara Eka Diana Puteri, Andi Tiara Eka Diana Anny Hary Ayu Apriana Vinasyiam Arbajayanti, Rahma Dini Asda Laining Asda Laining Audia Wuryas Pradita Negara, Grenda Ayi Rahmat Azis Kurniansyah B.A. Hasyim Dadang Syafruddin Darina Putri Darsiani Darsiani darsiani, Darsiani Deddy Yaniharto Deddy Yaniharto dedy yaniharto Dewi Sulasingkin Diamahesa, Wastu Ayu Didi Humaedi Yusuf, Didi Humaedi Dinamella Wahjuningrum Dini Harianto Dodi Hermawan E. Gandara Eddy Supriyono Enang Harris Enang Harris Enang Harris Enang Harris Enang Harris Enang Harris Endang Purnama Sari Fardila Putri, Rizqiyatul Febrina Rolin Fitriani, Farida Gumelar, Muhammad Raditya Hasan Abidin Hasan Nasrullah Hendriana, Andri Hestirianoto, Totok I Made Artika I MADE ARTIKA I Nyoman Adi Asmara Giri I. Mokoginta Ichsan Achmad Fauzi Ika Wahyuni Putri Imlani, Ainulyakin Hasan Imron Imron, Imron Ing Mokoginta Ing Mokoginta Ing Mokoginta Ing Mokoginta Ing Mokoginta ING MOKOGINTA Iqbal Kurniawinata, Mohamad Irzal Effendi Ismail Rahmat Ismarica, Ismarica Jefry Jefry Jr., Muhammad Zairin Juli Ekasari Julie Ekasari Jullie Ekasari Ketut Sugama Ketut Sugama Kukuh Nirmala M. Zairin Junior Mas Bayu Syamsunarno Mas Tri Djoko Sunarno Mas Tri Djoko Sunarno Mas Tri Djoko Sunarno, Mas Tri Djoko Meilisza, Nina Mia Setiawati Mohamad Amin MUHAMMAD AGUS SUPRAYUDI Muhammad Roikhan Amanullah Muhammad Zairin Jr. Muhammad Zairin Jr. Muhammad Zairin Jr. Mujizat Kawaroe MUNTI YUHANA Nadisa Theresia Putri Neltje Nobertine Palinggi Neltje Nobertine Palinggi nFN Safratilofa Nina Meilisza Nur Bambang Priyo Utomo Nur Bambang Priyo Utomo Nur Bambang Priyo Utomo Nur Hikma Mahasu Nur, Abidin NurBambang Priyono Utomo Nurina Pratiwi Odang Carman Otie Dylan Soebhakti Hasan Pangentasari, Dwinda R Rifai Rahma, Balqis Aulia Rahmayanti, Yessy Rakhmawati, Rakhmawati, Rasidi Rasidi Rasidi, Rasidi Reza Samsudin Ria Septy Anggraini, Ria Septy Ricky Ramadhan RIDWAN AFFANDI Rina Hirnawati Riska Diana Rizkan Fahmi Robin, , Shella Marlinda Shidik, Taufik Shidik Adi Nugroho Siti Komariyah Siti Murniasih Sri Nuryati Sri Nuryati Suardi Laheng Sumantri, Iwan Sumiana, I Kadek Syarifah Ruchyani Syefti Palmi, Revita T.L. Pelawi Talita Shofa Adestia Thoy Batun Citra Rahmadani Tira Silvianti Titin Kurniasih Toshiro Masumoto Triana Retno Palupi Tulas Aprilia Ucu Cahyadi Usman Usman Usman Usman Usman Usman Uttari Dewi Wahyu Pamungkas Wahyu Pamungkas Wahyudi, Imam Tri Wasjan Wasjan Wasjan WIDANARNI WIDANARNI Widya Puspitasari Wijianti, Hani Wiwik Hildayanti Yulfiperius, Yulfiperius Yulintine Yulintine YULISMAN Yuni Puji Hastuti Yutaka Haga Zuraida .