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All Journal HAYATI Journal of Biosciences MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management Jurnal Ilmu Pertanian Indonesia Jurnal Ilmu dan Teknologi Hasil Hutan Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Forum Pasca Sarjana Jurnal Sain Veteriner Biospecies Jurnal Veteriner ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Natur Indonesia Prosiding Seminar Biologi Microbiology Indonesia AL KAUNIYAH Jurnal Penelitian Hutan dan Konservasi Alam Jurnal Matematika & Sains agriTECH TREUBIA BERITA BIOLOGI JURNAL BIOLOGI INDONESIA ZOO INDONESIA Jurnal Biodjati BIOEDUSCIENCE Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Floribunda Jurnal Ilmu Kefarmasian Indonesia Konservasi Hayati Zoo Indonesia Proceeding Biology Education Conference Makara Journal of Science Jurnal Kedokteran Hewan Jurnal Kefarmasian Indonesia

TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN

Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor

Author-ID : 251065

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health Veterinary Other

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Keanekaragaman Genetik Rayap Tanah Genus Coptotermes (Isoptera: Rhinotermitidae) di Pulau Jawa Niken Subekti; Dodi Nandika; Dedy Duryadi Solihin
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 2 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.2.156

Subterranean termite Coptotermes spp. has been known as the most economically important structural pest in Indonesia. Due to morphological ambiguity, traditional identification of Coptotermes spp. has always been difficult and unreliable. Through molecular diagnostic method, a study was conducted to determine genetic variation of Coptotermes spp. occurring in Java Island. Termite specimens were collected from Banten 1, Banten 2, DKI 1, DKI 2, Jabar 1, Jabar 2, Jateng 1, Jateng 2, Yogya 1, Yogya 2, Jatim 1, and Jatim 2. The method for identification was PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis using four restriction enzymes each of which was applied to CO II amplicon for all the Coptotermes spp. being analyzed. The results showed the existence of two species of Coptotermes in Java which are different from both Coptotermes gestroi and Coptotermes formosanus.

Variasi Sekuens Gen Mitokondrial Sitokrom C Oksidase I dari Siput Lola (Trochus niloticus) Agus Nuryanto; Dedy Duryadi Solihin
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 1 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.1.143

A study on sequence variation in the mitochondrial DNA of Trochus niloticus has been conducted. Polymerase chain reactions (PCR) technique was applied to amplify cytochrome c oxidase I gene of 533 bp and ABI 310 aotomatic DNA sequencer was used to sequence the PCR product. This study was aimed to know the genetic variation of T. niloticus and the suitability of partial sequence of cytochrome c oxidase I gene as a molecular marker in the genetic study. The results showed that from two T. niloticus specimens, 12 polymorphic sites were detected yielding 2 haplotypes. This indicated a highly genetic variation in thecytochrome c oxidase I (COI) gene of T niloticus. In addition, cytochrome c oxidase I gene was proved to be a suitable marker for the genetics studies of various species of organisms.

Genetic Diversity of the Endangered Endemic Anoa (Bubalus spp): Implication for Conservation Dwi Sendi Priyono; Dedy Duryadi Solihin; Achmad Farajallah; Bambang Purwantara
HAYATI Journal of Biosciences Vol. 29 No. 5 (2022): September 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.5.586-596

Anoa is an endemic ungulate in Sulawesi and its status now is endangered because the population continues to decline. Conservation genetics is one of the crucial issues in the anoa conservation strategy and action plan 2013-2022 document, but this genetic data is not yet available. To investigate and provide valuable information for conservation genetics measures, thirteen polymorphic microsatellites were used to analyze 20 adult anoa. Anoa has relatively low genetic diversity within populations (HO = 0.58), and high genetic differentiation among populations (FST = 0157). Although the anoa population has a bottleneck signal (T.P.M: 0.019; P0.05), the bottleneck simulation results show that the loss of genetic diversity is being slow over the next 100 years (9.5%). We provide some recommendations for conservation genetics based on the findings in this paper, including monitoring and genetically mapping for other anoa populations due to bottleneck signals, establishing the founder of the ex-situ population by examining their genetic diversity status, maintaining and increasing the number of individuals in the ex-situ population to genetically safe population size, and managing anoa populations by avoiding inbreeding. In-situ and ex-situ conservation programs should be combined to maintain the genetic diversity of anoa.

Single Nucleotide Polymorphism within the Promoter Region of Cynomolgus Monkey LDLR Gene Achmad, Taher; Solihin, Dedy Duryadi; Sulistiyani, Sulistiyani; Astuti, Dewi Apri; Sajuthi, Dondin
Makara Journal of Science Vol. 22, No. 1
Publisher : UI Scholars Hub

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The cynomolgus monkey (Macaca fascicularis) is one of the most common nonhuman primates used as an animal model in biomedical research related to atherosclerosis. However, little is known about genetic variation in the low density lipoprotein receptor (LDLR) gene, its effect on cholesterol levels, and associated risks of atherosclerosis. There-fore this study aimed to identify genetic polymorphisms, namely single nucleotide polymorphisms (SNPs), within the promoter region of LDLR and their relationship with animal responsiveness to hypercholesterolemia due to an atherogenic diet. In this research, SNPs were studied using DNA isolated from22 cynomolgus monkeys obtained from aprevious study, consisting of two hyporesponders, 19 hyperresponders, and one extremecase. The result showed that two SNPs existed in the promoter region, namelyg.−169T>C and g.−265G>A. SNP g.−265G>A showed linkages with extreme responsiveness and can be used as a potential genetic marker for extreme animals. The result of this study has extended our knowledge of polymorphism in the LDLR geneand its use intheselection of cynomolgus monkeys as animal models in research on hypercholesterolemia and atherosclerosis.

CORRELATION BETWEEN ESTROGEN HORMONE CONCENTRATION AND ESTROUS CYCLE OF RAT FED SOYBEAN FLOUR AND TEMPEH FLOUR Safrida Safrida; Dedy Duryadi Solihin; Mustafa Sabri
Jurnal Kedokteran Hewan Vol 13, No 1 (2019): March
Publisher : Universitas Syiah Kuala

The objective of this research was to examine the correlation between estrogen level and estrous cycle of rat fed with soybean flour and tempeh flour. A completely randomized design was applied in this study with 6 treatment groups and 5 replications. Rats in group 1, 2, and 3 were non-ovariectomized rats which were fed with pellet, 10 g soybean flour/100 g body weight/day, and 10 g tempeh flour/ 100 g body weight/day, respectively. The rats in group 4, 5, qnd 6 were ovariectomized rat which were fed with pellet, 10 g soybean flour/100 g body weight/day, and 10 g tempeh flour/100 g body weight/day, respectively. The rats were after fed with pellet, soybean and tempeh flour for 4 weeks, the estrous cycle phases of rats were examined based on the presence of vaginal epithelial cells and the number of qualitative vaginal epithelial cells, while the estrogen concentrations in serum were measured by radioimmunoassay method. The data was analyzed using analysis of variance and followed by Duncan multiple range test at 95% confidence. Non-ovariectomized rats given tempeh flour have longer estrus cycle than the non-ovariectomized rats which given soybean flour. The isoflavon contained in tempeh flour and soybean flour induced proliferation and cornification of vaginal epithelial cell. Feeding the soybean flour and tempeh flour to ovariectomized rats could optimize the estrogen hormone in initiating the estrus phase, in which of the tempeh flour addition had better effect than soybean flour. Correlation between the level of estrogen to the length of estrous cycle in both nonovariectomized rats and ovariectomized rats was positive, in which the higher the levels of estrogen the longer the length of estrous cycle.

GENETIC VARIATION OF cDNA OF LDLR GENE IN HYPORESPONDER CYNOMOLGUS MACAQUES (Macaca fascicularis) Achmad Taher; Dedy Duryadi Solihin; Sulistiyani Sulistiyani; Dondin Sajuthi; Dewi Apri Astuti
Jurnal Kedokteran Hewan Vol 11, No 3 (2017): September
Publisher : Universitas Syiah Kuala

The study aimed to identify genetic variation of cDNA sequence from low density lipoprotein receptor (LDLR) gene of hyporesponder cynomolgus macaques. The animal used in this study was one hyporesponder cynomolgus macaque obtained from selection result in Primate Research Center-Bogor Institute of Agriculture (PSSP IPB). Amplification of cDNA from LDLR gene was performed using polymerase chain reaction (PCR) method with 4 pairs of walking primer. Alignment of amplification result sequence from 4 pairs of walking primer generated target sequence of 2353 bp which located on position 188-2540. Analysis of target sequence alignment on reference sequence in GenBank found 8 single nucleotide polymorphism (SNP), namely c408CT; c.1200CT; c.1497CT; c.1644TC; 1791TC; 1804AG; 2088CT; and 2377GA. Of 8 SNPs, c.1804AG and 2377GA. Two SNP (c.1804AG; dan 2377GA) caused changing of amino acids composition namely p.K602E (lysine → glutamate) and p.V793I (valine → isoleucine). This result proved the potential use of genetic variation of cDNA sequence from LDLR gene as genetic marker for selection of hyporesponder cynomolgus macaques.

istribution Patterns of the Morphology, Species, and Sex in the Stingray Species Complex of Himantura uarnak, Himantura undulata, and Himantura leoparda in Indonesia Arlyza1, Irma Shita; Solihin, Dedy Duryadi; Soedharma, Dedi
Makara Journal of Science Vol. 17, No. 2
Publisher : UI Scholars Hub

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The assessment of the morphology and stingray species distribution of the Himantura uarnak species complex covers eight sampling sites in western and eastern Indonesia as many as 113 individuals. The observation of the reticulate pattern was accomplished by directly checking the dorsal side of the stingrays, and growth observations also carried out. The distribution patterns were analyzed using the principal component analysis (PCA), while the growth patterns were evaluated using correlation analyses. The reticulation patterns of the H. uarnak species complex were highly variable, and the distribution patterns based on the morphological parameters, species, and sex produced four groups. The distribution of the length frequency of H. undulata and H. uarnak showed a range of different sizes. The body length of the female in the western region is relatively longer than the male, while in the eastern region the female revealed a range in relative size showing that the male was longer than the female. The length-weight relationships of the stingrays were negative allometric, indicating a b-value of less than 3. The b-values of both the male and female H. undulata were 1.5860 and 0.4380, respectively; while the male and female H. uarnak were 0.2956 and 0.4376, respectively.

Identification of Gene Resistance to Avian Influenza Virus (Mx Gene) among Wild Waterbirds Elfidasari, Dewi; Solihin, Dedy Duryadi; Soejoedono, Retno Damayanti; Murtini, Sri
Makara Journal of Science Vol. 17, No. 1
Publisher : UI Scholars Hub

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The Mx gene is an antiviral gene used to determine the resistance or the susceptibility to different types of viruses, including the Avian Influenza (AI) virus subtype H5N1. The AI virus subtype H5N1 infection in chickens causes Mx gene polymorphism. The Mx+ gene shows resistant to the AI virus subtype H5N1, whereas the Mxgene shows signs of susceptible. The objective of this research was to detect the Mx gene in wild aquatic birds using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method with the primer pairs F2 and NE-R2/R and the RsaI restriction enzyme. DNA samples were obtained from eight species of wild waterbirds with positive and negative exposure to the AI virus subtype H5N1. DNA amplification results showed that the Mx gene in wild aquatic birds is found in a 100 bp fragment, which is the same as the Mx gene found in chickens. However, unlike chickens, the Mx gene in wild aquatic birds did not show any polymorphism. This study proves that Mx- based resistance to AI virus subtype H5N1 in different in wild birds than in chickens.

SEROSURVEILLANCE OF AVIAN INFLUENZA VIRUS SUBTYPE H5N1 WITH HAEMAGGLUTINATION-INHIBITION ON WILD AQUATIC BIRDS IN PULAU DUA SERANG NATURAL RESERVES, BANTEN PROVINCE Elfidasari, Dewi; Solihin, Dedy Duryadi; Soejoedono, Retno Damayanti; Murtini, Sri; Noor, Yus Rusila
Makara Journal of Science Vol. 15, No. 2
Publisher : UI Scholars Hub

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Further detailed research is required to obtain deeper information on the role of wild birds on the distribution of Avian influenza in Asia. A research has been carried out on February–June 2007 focused on blood sampling (serosurveillance) of wild birds in Pulau Dua Nature Reserves (CAPD), Serang, Banten. The research is aimed to investigate the infection of AI virus sub-tye H5N1 on the studied wild birds. The blood samples were taken from studied aquatic birds, followed by HI (haemagglutination-inhibition) test. A total of 183 samples represent 7 water bird species were taken i.e Cattle egret Bubulcus ibis, Javan pond-heron Ardeola speciosa, Little egret Egretta garzetta, Intermediate egret Egretta intermedia, Black-crowned night heron Nycticorax nycticorax, Great egret Casmerodius albus and Grey heron Ardea cinerea. The result revealed that 41 (23.27%) samples showed the present of AIV antibodies serotype H5N1 which is identified as positive. Data showed 5 positive-test species, including B. ibis (29.27%), E. garzetta (29.27%), E. intermedia (4.88%), Ardeola speciosa (7.32%), and N. nycticorax (29.27%). A total of 41.46% were infected adult individual, whereas 58.54% were juveniles.

Bacterial and Fungal Communities in Tempeh as Reveal by Amplified Ribosomal Intergenic Sequence Analysis SEUMAHU, CECILIA ANNA; SUWANTO, ANTONIUS; RUSMANA, IMAN; SOLIHIN, DEDY DURYADI
HAYATI Journal of Biosciences Vol. 20 No. 2 (2013): June 2013
Publisher : Bogor Agricultural University, Indonesia

Tempeh is an Indonesian traditional fermented food produced using Rhizopus as a starter culture. In practice, however, the starter culture as well as fermentation processes would yield a polymicrobial fermentation, which generated a unique tempeh flavor and texture. This condition makes Indonesian tempeh as one of the most complex fermented food, while at the same time would make it difficult to scale up tempeh production with uniform quality and consistency. The aim of this study was to compare a number of tempeh microbial communities employing Amplified Ribosomal Intergenic Sequence Analysis (ARISA). Fresh tempeh samples were obtained from tempeh producers in Java and Moluccas. 16S rRNA gene libraries and DNA sequencing were employed to analyze further the nature of bacterial diversity in two selected tempeh samples. The results of our study showed that different tempeh producer possessed different Bacterial ARISA (BARISA) or fungi ARISA (FARISA) profiles. However, BARISA profiles were found to be more discriminative than FARISA, and therefore BARISA would be more useful for tempeh genetic fingerprint or barcoding.

Co-Authors Abdul Rahman Singkam Abdul Rahman Singkam Achmad - Taher ACHMAD FARAJALLAH Achmad Machmud Achmad Machmud Thohari Achmad Taher Achmad Taher Achmad, Taher Agus Nuryanto Agus Wahyana Anggara Agus Wahyana Anggara Agus Wahyana Anggara, Agus Wahyana Alam Putra Persada Ani Mardiastuti Ani Suryani Antonius Suwanto Any Aryani Ardi Kapahang Arief Boediono Arlyza1, Irma Shita Arzyana Sunkar Bahiyah Bahiyah Bambang Purwantara Cece Sumantri CECILIA ANNA SEUMAHU CECILIA ANNA SEUMAHU CHRISTIAN HANSJOACHIM SCHULZE D.D. Sastraatmadja DAMAYANTI BUCHORI Daniel Happy Putra Dedi . Soedharma Dedi Soedharma DEDI SOEDHARMA DEWI APRI ASTUTI Dewi Elfidasari Dewi Malia Prawiradilaga Dewi Malia Prawiradilaga, Dewi Malia DIAH ISKANDRIATI Diah Iskandriati DIETMAR BLOHM Dodi Nandika Dondin Sajuthi Dwi ASTUTI Dwi Astuti Dwi Sendi Priyono DYAH PERWITASARI -FARAJALLAH Epa Paujiah, Epa Evy Ayu Arida EVY AYU ARIDA Fahma Wijayanti Fahri Fahrudin, Fahri Faisal Mustafa Findra, Muhammad Nur FUNGKEY HOETAMA Gita Kusuma Rahayu Hadi Allikodra Hadi S Alikodra HAJRIAL ASWIDINNOOR Handayani , Handayani Handayani Handayani Hari Prayogo Harini Nurcahya Mariandayani Heddy Julistiono HEDDY JULISTIONO Hermanu Triwidodo I Gusti Agung Arta Putra I WAYAN SUANA Ibnu Maryanto Iman Rusmana Indah Fajarwati, Indah Irma Shita Arlyza Irzaman, Irzaman Isdradjad Setyobudiandi Ismayati Afifah Jakaria Jakaria Jamhari Jamhari Jarulis Jarulis Jarulis Jarulis Jarulis Jarulis Jito Jito Jito Sugardjito Jusmaldi Jusmaldi Kadarwan Soewardi Khustina, Yenny Chusna Khustina, Yenny Chusna Kunio Watanabe Lia Aseptin Murdini Lilik Budi Lilik Budi Prasetyo LILIK BUDIPRASETYO Lucia Johana Lambey M F Rahardjo M. F. Rahardjo M. Zairin Junior Mahmud, Rois MARIA BINTANG MF Rahardjo Mustafa Sabri Nastiti Kusumorini NEVIATY PUTRI ZAMANI Niken Subekti Niken TM Pratiwi Nurlisa Alias Butet Pamungkas, Joko Prasetyo Prasetyo Priyono, Siti N. Puji Rianti Retno Damayanti Soejoedono RICHARD F GRANT RIDWAN AFFANDI Rini Widayanti Robba Fahrisy Darus Roedhy Poerwanto Roedy Poerwanto Roedy Poerwanto Roni Koneri Ronny Rachman Noor Roza Elvyra Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudi Afnan Rudi Tarumingkeng Rudy C Tarumingkeng Safrida Safrida Safrida Safrida Saroyo Saroyo SATA YOSHIDA SRIE RAHAYU SATRIYAS ILYAS SELA SEPTIMA MARIYA SILMI MARIYA Siti N. Priyono SJAFRIDA MANUWOTO Soaloon Sinaga Soaloon Sinaga Sobir Sobir Sobir Sobir Sobir Sobir Solihin Solihin Sri Catur Setyawatiningsih Sri Catur Setyawatiningsih sri murtini . SRI NINGSIH Sri Sulandari Sri Supraptini Mansjoer Subyakto Subyakto Sugardjito Sugardjito Suharsono Suharsono SULISTIYANI SULISTIYANI SULISTIYANI SULISTIYANI Sulistiyani Sulistiyani Sulistiyani, Sulistiyani Surjono Surjokusumo Syaiful Anwar Syamsul Bachry Taher, Achmad Tedjo Sukmono Thohari Thohari Tike Sartika Tri Haryoko Tri Haryoko, Tri Tutik Wresdiyati UUS SAEPULOH UUS SAEPULOH Wasmen Manalu Yuli Wahyu Tri Mulyani YULIN LESTARI Yuni Cahya Endrawati Yus Rusila Noor Yus Rusila Noor Yusnarti Yus

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