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All Journal HAYATI Journal of Biosciences MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management Jurnal Ilmu Pertanian Indonesia Jurnal Ilmu dan Teknologi Hasil Hutan Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Forum Pasca Sarjana Jurnal Sain Veteriner Biospecies Jurnal Veteriner ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Natur Indonesia Prosiding Seminar Biologi Microbiology Indonesia AL KAUNIYAH Jurnal Penelitian Hutan dan Konservasi Alam Jurnal Matematika & Sains agriTECH TREUBIA BERITA BIOLOGI JURNAL BIOLOGI INDONESIA ZOO INDONESIA Jurnal Biodjati BIOEDUSCIENCE Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Floribunda Jurnal Ilmu Kefarmasian Indonesia Konservasi Hayati Zoo Indonesia Proceeding Biology Education Conference Makara Journal of Science Jurnal Kedokteran Hewan Jurnal Kefarmasian Indonesia
TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health Veterinary Other

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CORRELATION BETWEEN ESTROGEN HORMONE CONCENTRATION AND ESTROUS CYCLE OF RAT FED SOYBEAN FLOUR AND TEMPEH FLOUR Safrida, Safrida; Solihin, Dedy Duryadi; Sabri, Mustafa
Jurnal Kedokteran Hewan Vol 13, No 1 (2019): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v13i1.12631

Abstract

The objective of this research was to examine the correlation between estrogen level and estrous cycle of rat fed with soybean flour and tempeh flour. A completely randomized design was applied in this study with 6 treatment groups and 5 replications. Rats in group 1, 2, and 3 were non-ovariectomized rats which were fed with pellet, 10 g soybean flour/100 g body weight/day, and 10 g tempeh flour/ 100 g body weight/day, respectively. The rats in group 4, 5, qnd 6 were ovariectomized rat which were fed with pellet, 10 g soybean flour/100 g body weight/day, and 10 g tempeh flour/100 g body weight/day, respectively. The rats were after fed with pellet, soybean and tempeh flour for 4 weeks, the estrous cycle phases of rats were examined based on the presence of vaginal epithelial cells and the number of qualitative vaginal epithelial cells, while the estrogen concentrations in serum were measured by radioimmunoassay method. The data was analyzed using analysis of variance and followed by Duncan multiple range test at 95% confidence. Non-ovariectomized rats given tempeh flour have longer estrus cycle than the non-ovariectomized rats which given soybean flour. The isoflavon contained in tempeh flour and soybean flour induced proliferation and cornification of vaginal epithelial cell. Feeding the soybean flour and tempeh flour to ovariectomized rats could optimize the estrogen hormone in initiating the estrus phase, in which of the tempeh flour addition had better effect than soybean flour. Correlation between the level of estrogen to the length of estrous cycle in both nonovariectomized rats and ovariectomized rats was positive, in which the higher the levels of estrogen the longer the length of estrous cycle.
GENETIC VARIATION OF cDNA OF LDLR GENE IN HYPORESPONDER CYNOMOLGUS MACAQUES (Macaca fascicularis) Taher, Achmad; Solihin, Dedy Duryadi; Sulistiyani, Sulistiyani; Sajuthi, Dondin; Astuti, Dewi Apri
Jurnal Kedokteran Hewan Vol 11, No 3 (2017): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i3.2939

Abstract

The study aimed to identify genetic variation of cDNA sequence from low density lipoprotein receptor (LDLR) gene of hyporesponder cynomolgus macaques. The animal used in this study was one hyporesponder cynomolgus macaque obtained from selection result in Primate Research Center-Bogor Institute of Agriculture (PSSP IPB). Amplification of cDNA from LDLR gene was performed using polymerase chain reaction (PCR) method with 4 pairs of walking primer. Alignment of amplification result sequence from 4 pairs of walking primer generated target sequence of 2353 bp which located on position 188-2540. Analysis of target sequence alignment on reference sequence in GenBank found 8 single nucleotide polymorphism (SNP), namely c408CT; c.1200CT; c.1497CT; c.1644TC; 1791TC; 1804AG; 2088CT; and 2377GA. Of 8 SNPs, c.1804AG and 2377GA. Two SNP (c.1804AG; dan 2377GA) caused changing of amino acids composition namely p.K602E (lysine glutamate) and p.V793I (valine isoleucine). This result proved the potential use of genetic variation of cDNA sequence from LDLR gene as genetic marker for selection of hyporesponder cynomolgus macaques.
Modeling The Habitat Suitability Of Javan Banteng (Bos javanicus javanicus) Using Geographic Information System In Ujung Kulon National Park Handayani , Handayani; Solihin, Dedy Duryadi; Alikodra, Hadi Sukadi; Jakaria, Jakaria; Rianti, Puji; Mahmud, Rois
BIOEDUSCIENCE Vol 7 No 3 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11547

Abstract

Background: The Banteng population in Ujung Kulon National Park (TNUK) is less than 500 individuals. The habitat of Java Banteng in conservation areas has largely decreased. One approach to assessing the current status of biodiversity at all levels, especially in endangered species, is to use geospatial technology such as remote sensing and geographic information systems combined with spatial data science. This study aims to create a spatial model of the suitability of the Javan Banteng habitat in the TNUK area and identify the use of the Java Banteng habitat and environmental variables that affect the presence of Javan Banteng. Methods: This research data was collected through coordinate data for stool sampling and data from BTNUK using a method called maximum entropy (maximum). The analysis used the Relative Use Index, Maximum Entropy modeling, and Relative Abundance Index. Results: Based on research, the use of habitat by Java Banteng with the value of making a spatial distribution model can be analyzed by analyzing the contribution of environmental variables based on the level of contribution in percent and the results of the jackknife test, namely the percentage of contribution of environmental variables in this study showed that environmental parameters, slope (37.6%) were the highest parameters, followed elevation (25.8%), land cover (25.3%), and NDVI (6%),  rivers (5.3%). The analysis of five environmental variables used in making the Javan Banteng distribution model showed that at an altitude of 45 meters above sea level, Java Banteng preferred to show 95%. The graph decreased at an altitude above 45 mdpl, and Java Banteng at 200 meters above sea level looked at 0%. Conclusions: Javan Banteng do not like or do not choose places with altitudes ranging from 200 - 625 meters above sea level.
Keragaman Morfometrik dan Gen cytochrome b DNA Mitokondria Ompok hypopthalmus di DAS Batang Hari. Singkam, Abdul Rahman; Solihin, Dedy Duryadi; Affandi, Ridwan
Biospecies Vol. 11 No. 2 (2018): Juli 2018
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.702 KB) | DOI: 10.22437/biospecies.v11i2.5724

Abstract

This research aims to analyse the variation on morphometric and cytochrome b (cyt b) DNA mitocondria gene of Ompok hypopthalmusin Batang Hari river. Cyt b DNA mitocondria gene was amplified by polymerase chain reactionand targeted1104bp nucleotids. The results show there is no significant differentiation on morphometric structure of the samples. This result is consistent for both of morphometric (p=0.96) and index (p=0.99). Amplification of cyt b DNA mitocondria gene generates927 bp nucleotids with cytosin as the majour component and guanin as the lowest. Only one nucleotide(0.11%) is vary among all the sequences i.e.883thof the partial cyt b gene. The 883th nucleotidein O. hypopthalmus Simpang and Sungai Bengkal is guanin, while inMandiangin dan Pelayangan is adenin. This subtitution occured on the first codon and changed the 295th putative amino acid ofcyt b gene. The putative amino acid in O. hypopthalmus Simpang-Sungai Bengkal is alanina (GCC), while inMandiangin-Pelayangan is treonina (ACC).
Gambier for Diabetes: Comparison of the Antidiabetic Potency between Two Types of Extracts from Uncaria gambir (W. Hunter) Roxb. Fajarwati, Indah; Solihin, Dedy Duryadi; Wresdiyati, Tutik
Jurnal Kefarmasian Indonesia VOLUME 14, NUMBER 1, FEBRUARY 2024
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Gambier is a dry extract obtained from both the leaves and twigs of the Uncaria gambir. It is an agricultural product produced by the local community through traditionally unique methods. The main objective of this study was to evaluate the antidiabetic potency of gambier. The study used two types of gambier, namely traditional aqueous gambier (TAG) from traditional extraction and maceration ethanol gambier (MEG) from maceration extraction. The phytochemical compound, alpha-glucosidase inhibition, and antioxidant activity were analyzed. Moreover, in vivo hypoglycemia activity study was carried out. The results showed that both TAG and MEG contained flavonoids, tannin, and triterpenoids, while saponins were detected in TAG and steroids in MEG only. Both TAG and MEG also contained quercetin and catechin which were higher in the TAG compared to the MEG. The DPPH scavenging activities of the TAG and MEG, measured in IC50 values, were 15.40 ± 0.21 μg/ml and 12.25 ± 0.04 μg/ml, respectively. The IC50 values of the alpha-glucosidase inhibition of TAG and MEG were 35.84 ± 1.75 μg/ml and 83.14 ± 1.26 μg/ml, respectively. The best hypoglycemic activity was observed in the TAG group. The group given TAG and high glucose had lower blood glucose (127 mg/dl) compared to the group only given high glucose (178 mg/dl). These results indicated that TAG had a better potential for antidiabetic activities compared to MEG, and it can be proposed for further investigations as a potential antidiabetic agent.
Co-Authors Abdul Rahman Singkam Abdul Rahman Singkam Achmad - Taher ACHMAD FARAJALLAH Achmad Machmud Achmad Machmud Thohari Achmad Taher Achmad Taher Achmad, Taher Agus Nuryanto Agus Wahyana Anggara Agus Wahyana Anggara Agus Wahyana Anggara, Agus Wahyana Alam Putra Persada Ani Mardiastuti Ani Suryani Antonius Suwanto Any Aryani Ardi Kapahang Arief Boediono Arlyza1, Irma Shita Arzyana Sunkar Bahiyah Bahiyah Bambang Purwantara Cece Sumantri CECILIA ANNA SEUMAHU CECILIA ANNA SEUMAHU CHRISTIAN HANSJOACHIM SCHULZE D.D. Sastraatmadja DAMAYANTI BUCHORI Daniel Happy Putra Dedi . Soedharma DEDI SOEDHARMA Dedi Soedharma DEWI APRI ASTUTI Dewi Elfidasari Dewi Malia Prawiradilaga Dewi Malia Prawiradilaga, Dewi Malia Diah Iskandriati DIAH ISKANDRIATI DIETMAR BLOHM Dodi Nandika Dondin Sajuthi Dwi ASTUTI Dwi Astuti Dwi Sendi Priyono DYAH PERWITASARI -FARAJALLAH Epa Paujiah, Epa Evy Ayu Arida EVY AYU ARIDA Fahma Wijayanti Fahri Fahrudin, Fahri Faisal Mustafa Findra, Muhammad Nur FUNGKEY HOETAMA Gita Kusuma Rahayu Hadi Allikodra Hadi S Alikodra HAJRIAL ASWIDINNOOR Handayani , Handayani Handayani Handayani Hari Prayogo Harini Nurcahya Mariandayani Heddy Julistiono HEDDY JULISTIONO Hermanu Triwidodo I Gusti Agung Arta Putra I WAYAN SUANA Ibnu Maryanto Iman Rusmana Indah Fajarwati, Indah Irma Shita Arlyza Irzaman, Irzaman Isdradjad Setyobudiandi Ismayati Afifah Jakaria Jakaria Jamhari Jamhari Jarulis Jarulis Jarulis Jarulis Jarulis Jarulis Jito Jito Jito Sugardjito Jusmaldi Jusmaldi Kadarwan Soewardi Khustina, Yenny Chusna Khustina, Yenny Chusna Kunio Watanabe Lia Aseptin Murdini Lilik Budi Lilik Budi Prasetyo LILIK BUDIPRASETYO Lucia Johana Lambey M F Rahardjo M. F. Rahardjo M. Zairin Junior Mahmud, Rois MARIA BINTANG MF Rahardjo Mustafa Sabri Nastiti Kusumorini NEVIATY PUTRI ZAMANI Niken Subekti Niken TM Pratiwi Nurlisa Alias Butet Pamungkas, Joko Prasetyo Prasetyo Priyono, Siti N. Puji Rianti Retno Damayanti Soejoedono RICHARD F GRANT RIDWAN AFFANDI Rini Widayanti Robba Fahrisy Darus Roedhy Poerwanto Roedy Poerwanto Roedy Poerwanto Roni Koneri Ronny Rachman Noor Roza Elvyra Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudi Afnan Rudi Tarumingkeng Rudy C Tarumingkeng Safrida Safrida Safrida Safrida Saroyo Saroyo SATA YOSHIDA SRIE RAHAYU SATRIYAS ILYAS SELA SEPTIMA MARIYA SILMI MARIYA Siti N. Priyono SJAFRIDA MANUWOTO Soaloon Sinaga Soaloon Sinaga Sobir Sobir Sobir Sobir Sobir Sobir Solihin Solihin Sri Catur Setyawatiningsih Sri Catur Setyawatiningsih sri murtini . SRI NINGSIH Sri Sulandari Sri Supraptini Mansjoer Subyakto Subyakto Sugardjito Sugardjito Suharsono Suharsono SULISTIYANI SULISTIYANI SULISTIYANI SULISTIYANI Sulistiyani Sulistiyani Sulistiyani, Sulistiyani Surjono Surjokusumo Syaiful Anwar Syamsul Bachry Taher, Achmad Tedjo Sukmono Thohari Thohari Tike Sartika Tri Haryoko Tri Haryoko, Tri Tutik Wresdiyati UUS SAEPULOH UUS SAEPULOH Wasmen Manalu Yuli Wahyu Tri Mulyani YULIN LESTARI Yuni Cahya Endrawati Yus Rusila Noor Yus Rusila Noor Yusnarti Yus