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In Vitro Antibacterial Activity of Black Soldier Fly (Hermetia Illucens) Larva Extracts Against Gram-Negative Bacteria Harlystiarini Harlystiarini; Rita Mutia; I Wayan Teguh Wibawan; Dewi Apri Astuti
Buletin Peternakan Vol 43, No 2 (2019): BULETIN PETERNAKAN VOL. 43 (2) MAY 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v43i2.42833

Abstract

The aim of this study was to evaluate the in vitro antibacterial activity of Black soldier fly (BSF) larva extract. The BSF larva was extracted using methanol and then tested for antibacterial activity using agar diffusion method (zone growth inhibition). The antibacterial activity was conducted against Salmonella sp. and Escherichia coli, two important bacterial strains in poultry, using six dilution levels (10 mg/ml, 20 mg/ml, 40 mg/ml, 80 mg/ml, 160 mg/ml and 320 mg/ml). All the results were subjected analyze using t-test method. Based on the diameter of the inhibition zone, the BSF larva extract has a strong (P<0.05) antibacterial activity against Salmonella sp. and E. coli when the concentration used 320 mg/ml. In addition, BSF larva extract also contain high amount of lauric acid (49.18%), a saturated fatty acid that has been proven to proposes as antibacterial agent. Therefore, it could be concluded that the BSF larva extract could be used as a candidate for antibacterial substances. 
Profil Hematologi dan Status Metabolit Darah Domba Garut yang Diberi Pakan Limbah Tauge pada Pagi atau Sore Hari (BLOOD HAEMATOLOGICAL PROFILE AND METABOLITE STATUS OF GARUT LAMB FED DIETS MUNG BEAN SPROUT WASTE IN THE MORNING OR EVENING) Sri Rahayu; Mohamad Yamin; Cece Sumantri; Dewi Apri Astuti
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (92.712 KB) | DOI: 10.19087/jveteriner.2017.18.1.38

Abstract

The aim of this study was to evaluate the effect of diet containing mung bean sprout waste (MBSW) and feeding times on the hematological profiles and metabolite status of garut lambs. Experimental design used in this study was a factorial randomized block design with two factors of diet and two feeding times. Two factors of diets were 60% concentrate 1+40% natural grass and 60% concentrate 2+40% MBSW. Feeding times were in the morning (6:00-7:00 am) or the evening (5:00-6:00 pm). The animals were reared in individual cages and fed with 5% DM/kg body weight/day. The hematological profiles of the goat were examined and analyzed with Anova. The results showed the treatment of rations significant effect on blood glucose, whereas feeding time very significant to glucose, erythrocytes and hemoglobin, and the interaction of both treatments significantly affect hemoglobin, hematocrit, and glucose level. Meanwhile,leukocytes, neutrophils, lymphocytes, neutrophils/lymphocyte ratio (N/L), urea-N, and blood cholesterol were not affected by the treatments and their interactions. But overall the number of erythrocytes 8.72-12.78x106/mm3, hemoglobin 7.6-10.02 g/dL, leukocytes 8.94-12.27x103/mm3, neutrophils 33.00-52.20%, lymphocytes 38.80-52.40%, N/L 0.78-1.66, glucose 41.94-54.24 mg/dL, urea-N 29.91-35.87 mg/dL, and blood cholesterol 24.57-30.28 mg/dL. These results suggest that diets containing 40% MBSW together with a morning or an evening feeding time did not cause disturbances in haematological profile and blood metabolite status of garut lamb. ABSTRAK Penelitian ini bertujuan untuk mengevaluasi efek ransum mengandung hijauan limbah tauge dan waktu pemberian yang berbeda terhadap profil hematologi dan status metabolit darah domba garut. Materi penelitian berupa 20 ekor domba garut berumur 6-7 bulan (bobot badan 15,42 ± 2,42 kg) dipelihara selama tiga bulan dan dikandangkan secara individu serta diberi pakan sebanyak 5% bahan kering/kg berat badan/hari. Penelitian ini menggunakan rancangan acak kelompok pola faktorial dengan dua perlakuan yaitu jenis ransum dan waktu pemberian pakan. Dua jenis ransum yang diformulasikan dengan basis bahan kering (BK) adalah Ransum 1 (60% konsentrat 1 + 40% rumput lapang) dan Ransum 2 (60% konsentrat 2 + 40% limbah tauge). Waktu pemberian pakan terdiri dari pemberian pakan pada pagi hari (06.00-07.00) dan sore hari (17.00-18.00). Pengambilan sampel darah dilakukan pada akhir bulan kedua setelah masa pemeliharaan. Data yang diperoleh dianalisis dengan sidik ragam. Perlakuan jenis ransum berpengaruh nyata terhadap kadar glukosa darah. Waktu pemberian pakan berpengaruh nyata dan sangat nyata terhadap kadar glukosa, eritrosit dan hemoglobin. Interaksi kedua perlakuan tersebut berpengaruh nyata terhadap hemoglobin, hematokrit, dan glukosa darah. Leukosit, neutrofil, limfosit, rasio neutrofil/limfosit (N/L), urea-N, dan kolesterol darah tidak dipengaruhi oleh kedua perlakuan dan interaksinya. Secara keseluruhan jumlah eritrosit 8,72-12,78 x 106/mL, hemoglobin 7,6-10,02 g/dL, leukosit 8,94-12,27 x 103/mL, neutrofil 33,00-52,20%, limfosit 38,80-52,40%, N/L 0,78-1,66, glukosa 41,94-54,24 mg/dL, urea-N 29,91-35,87 mg/dL, dan kolesterol darah 24,57-30,28 mg/dL. Simpulan penelitian adalah ransum yang mengandung 40% limbah tauge dengan waktu pemberian pakan pagi atau sore hari tidak menyebabkan gangguan pada profil hematologis dan status metabolit darah domba garut.
Tanggap Kebal dan Tampilan Produksi Ayam Pedaging yang Diberi Ekstrak Buah Mengkudu (IMMUME RESPONSE AND PRODUCTION PERFORMANCE OF BROILERS GIVEN NONI FRUIT EXTRACTS) Razak Achmad Hamzah; Ida Wiryanti; Dewi Apri Astuti; Fadjar Satrija
Jurnal Veteriner Vol 13 No 1 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Noni fruit (Morinda citrifolia) has been used extensively as a drug, which is advertised with a varietyof benefits, such as can increase endurance, improve productivity. Those statements have not been provedscientifically. The results of this study, are expected to provide some information to the public regardingthe true benefits of Morinda citrifolia. The aims of this research were to study the effect of Noni (Morindacitrifolia) fruit extract given to broilers to their antibody titers and the counts of lymphocytes, basophils,eosinophils, monocytes, heterophils, and leukocytes. Production performance of these chickens was alsostudied. One hundred and sixty Cobb’s strain of broilers were used in this researh and were divided intofour groups of treatments which were divided into four experiment units which each comprised of tenchickens. Mengkudu fruit extract was given for 14 consecutive days orally using doses 0.0 g, 0.1 g , 0.5 g, and1.0 g per kilogram of body weight (kbw). The chickens utilized as control (0.0 g /gbw) were given only thesame plain water which was used to extract the fruits. The blood sampling were collected at the 3rd, 5th, 14th,and 21st days of the research. The study showed mengkudu fruit extract given at dose of 0.5 g/kbw was ableto elevate immune response (antibody) significantly if given in seven succesive days, and maintain toperform as an antiallergic when the extract given at dose 0.1, 0.5, dan 1.0 g/kww for 5 consecutive days. Butthe effect on the production performance had never been observed at all level of the dose and duration ofobservation.
Variasi Kolesterol Plasma Individual Monyet Ekor Panjang (Macaca fascicularis) sebagai Respons terhadap Diet Aterogenik IPB-1 [INDIVIDUAL PLASMA CHOLESTEROL VARIATION OF CYNOMOLGUS MACAQUE (MACACA FASCICULARIS) IN RESPONSE TO IPB-1 ATHEROGENIC DIET] Achmad Taher; Dedy Duryadi Solihin; Sulistiyani Sulistiyani; Dondin Sajuthi; Dewi Apri Astuti
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The effectiveness of atherogenic diet in raising plasma cholesterol level of cynomolgus macaques(Macaca fascicularis) can be predicted for population, but not for individuals.This study aimed to evaluatethe individual plasma cholesterol of cynomolgus macaques in response to IPB-1 atherogenic diet. Theanimals under study were 22 adult malemonkeys from the animal facility of Primate Research CenterBogor Agricultural University (PSSP IPB). All animals were intervened with the IPB-1 atherogenic diet forthree months and individual plasma cholesterol wasevaluated in a monthly basis.The results showedthat the monkeys’ plasma cholesterol had increased significantly (P <0.05) after one month of interventionperiod and the increases were very significantly different (P <0.01) after three months. Individually, increasesin plasma cholesterol varied among animals. Based on these variations, 2 animals were categorised ashypo-response, 18 animals as hyper-response and 2 animals as extreme.This study showed the variationof individual plasma cholesterol of cynomolgus macaques in response tothe IPB-1 atherogenic diet so thatthe animals might be selected based on the their responsiveness.
Prevalensi dan Faktor Risiko Infeksi Parasit Saluran Pencernaan pada Kerbau Lumpur di Kabupaten Brebes, Jawa Tengah Nanis Nurhidayah; Fadjar Satrija; Elok Budi Retnani; Dewi Apri Astuti; Sri Murtini
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (249.598 KB) | DOI: 10.19087/jveteriner.2019.20.4.572

Abstract

Kerbau lumpur merupakan ternak multiguna yang memiliki kedekatan dengan kehidupan masyarakat di kawasan lumbung ternak khususnya di Kabupaten Brebes, Jawa Tengah. Infeksi parasit saluran pencernaan pada kerbau lumpur bedampak negatif terhadap kesehatan ternak sekaligus menyebabkan kerugian ekonomi. Data epidemiologi infeksi saluran pencernaan pada kerbau lumpur di Kabupaten Brebes belum tersedia. Penelitian ini bertujuan untuk mengukur prevalensi, derajat infeksi serta analisis faktor risiko infeksi parasit saluran pencernaan pada kerbau lumpur di Kabupaten Brebes. Sebanyak 240 sampel tinja diperoleh dari kerbau di empat kecamatan, yaitu; Salem, Bantarkawung, Tonjong dan Brebes. Hasil pemeriksaan mikroskopik dengan teknik Mc Master menunjukkan bahwa 27.5% kerbau terinfeksi oleh parasit saluran pencernaan. Infeksi disebabkan oleh Toxocara vitulorum (1.67%; telur tiap gram tinja [TTGT] 174.4), cacing Strongyle (Cooperia, Nematodirrus, Trichostrongylus) (4.17%; TTGT 140.18), Trichuris spp. (3.33%; TTGT 64.84), Moniezia expansa (9.17%; TTGT 122.47) dan Eimeria spp (21.25%; TTGT 162.37). Infeksi lebih tinggi tercatat pada kelompok jantan, umur muda (>12–30 bulan), kerbau di Kecamatan Salem dan yang dipelihara pada daerah bertipe iklim D4. Variabel umur merupakan satu-satunya faktor risiko infeksi yang ditunjukkan dengan ­P-value 0.03. Nilai odd ratio kelompok muda dan pedet adalah 3.424 dan 2.529, artinya, kelompok muda dan pedet memiliki risiko 3.424 dan 2.529 lebih tinggi dibandingkan kerbau dewasa (referensi). Dari penelitian ini dapat disimpulkan, baik prevalensi maupun derajat infeksi parasit saluran pencernaan di Kabupaten Brebes terjadi dalam taraf yang rendah dan dipengaruhi oleh faktor umur ternak.
Penurunan Kadar Gula Darah pada Monyet Ekor Panjang Obes dengan Pemberian Nikotin Dosis Rendah (DECRESE IN BLOOD SUGAR LEVEL IN LONG TAILED OBESE MACAQUES GIVING WITH LOW DOSE NICOTINE) Chusnul Choliq; Dondin Sajuthi; Irma Herawati Suparto; Dewi Apri Astuti; Retno Wulansari
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of the study was to evaluate the role of low dose nicotine on the profile of blood glucose and  â-cell of pancreatic islets.  Fourteen adult (aged 6 – 8 years) male cynomolgus monkeys grouped based ontheir Body Mass Index (BMI) into preobese (BMI=23.65 – 25.00) and obese (BMI e” 26.00) were used inthis study. Subsequently animals were grouped into four:  (i) preobese monkeys with nicotine (pOb+), (ii)obese monkeys with nicotine (Ob+), (iii) preobese monkeys without nicotine (pOb-), and (iv) obese monkeyswithout nicotine (Ob-). Animals in the nicotine groups were fed with high fat diet mixed with nicotine dose0.5–0.75mg/kg body weight/day for three months and the others were fed Monkey Chow® only as thecontrol group. Blood samples were collected every month for glucose analysis and necropsy was performedat the end of study. Pancreas tissues were processed histologically and stained using  immunohistochemicalmethod. The results showed that the blood glucose either preobese (28.37%) or  obese (33.72%) animals inthe nicotine groups significantly decrease (p<0.05) during the study period in comparison to the controlgroup.  Based on brown color intensity of granules cytoplasm of insulin producing cells or immunoreactiveâ-cells, it is shown that the cells of animals in the non nicotine group were more reactive than those in thenicotine groups. In conclusion, there was positive effect of low dose nicotine in maintaining the bloodglucose level in normal range by stimulation of islet cells proliferation to maintain the production ofinsulin in the pancreatic islet.
Assessing the Effectiveness of Top Leaf Meal of Indigofera zollingeriana to Substitute Soybean Meal through Evaluation on Protein Quality and Metabolic Energy in Poultry Feed Rizki Palupi; Sumiati Sumiati; Dewi Apri Astuti; Luki Abdullah
IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Vol 3, No 2 (2018): June 2018
Publisher : IJFAC (Indonesian Journal of Fundamental and Applied Chemistry)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24845/ijfac.v3.i2.47

Abstract

This study aimed to evaluate protein quality of top leaf meal of Indigofera zollingerina based on its net protein utilization (NPU) as well as to evaluate its metabolizable energy. Fifty broiler chicks were used in determination of NPU while fifteen broiler strain Cobbs chickens aged 5 weeks were used for metabolizable energy measurement. This study used descriptive method in which data obtained were analyzed and compared to NPU and metabolizable energy of soybean meal. The results showed that top leaf meal of Indigoferazollingerinahad NPU of 38.58 – 46.98, which is 70.14% - 85-42% of that of soybean meal (55). Its metabolizable energy was 2791.12 kcal/kg and was 9.46% higher than that of soybean meal. As the NPU and metabolizable energy were close to those of soybean meal, top leaf meal of Indigoferazollingerinacan be used to substitute protein from soybean meal in poultry feed.
The effect of bean sprout waste and feeding time to the chemical composition of Garut lamb Siti Aslimah; Mohammad Yamin; Dewi Apri Astuti
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 29, No 3 (2019): Desember
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2019.029.03.03

Abstract

This research aims to determine the chemical composition of Garut lamb fed with bean sprout waste along with different feeding time. The subjects of this      research were 12 Garut sheep aged 6 to 7 months with the initial weight at 16.76 ± 2.09 kg (co-efficient of variation = 12.47%). Both of the given feed were consisted of 14% isopro-tein and 65% iso-total digestible nutrients (iso-TDN), which divided into R1 (40% for-age + 60% concentrate 1) and R2 (40% bean sprout waste + 60% concentrate 2). The research was conducted in a completely randomized design with 2x2 factorial and rep-licated 3 times. The first factor was two feed types (R1 and R2), while the      second factor was the feeding time (morning and afternoon). The obtained chemical composi-tion data of lamb were then analyzed with ANOVA. The results showed that the chem-ical composition of Garut lamb fed with bean sprout waste was similar to the lamb fed with forage. Moreover, the different feeding time did not show significant   differences as well (P>0.05), and there was no interaction between type of feed with feeding time to the chemical composition of Garut meat. The water, fat, protein, and ash content of Garut lamb fed with bean sprout waste were 77.09 ± 2.07%; 1.60 ± 0.69%; 16.66 ± 0.38%; and 0.42 ± 0.41%, while fed with forage were 73.25 ± 1.66%; 3.27 ± 2.02%; 17.68 ± 1.82%; and 0.48 ± 0.20%, respectively.
Supplementation of black soldier fly (Hermetia illucens) on productivity and blood hematology Andri Cahya Irawan; Dewi Apri Astuti; I Wayan Teguh Wibawan; Widya Hermana
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 30, No 1 (2020): April 2020
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2020.030.01.06

Abstract

Black Soldier Fly larvae are natural antibiotics. The use of methanol extract in BSF larvae has a dual function, namely high protein content and high antibiotic content to kill Gram-negative bacteria. The biological structure of BSF larvae with antimicrobial peptide (AMP) acts as an inhibitor of pathogenic microorganisms, has a high lauric acid function as a natural antimicrobial and chitin, the polysaccharide plays a role in enhancing the immune response of the animal. This study aims to gain the best type of BSF protein from feed treatments containing live BSF, dry BSF, and BSF extract to increase blood productivity and hematology. The results of this study show that the best P3 treatment for the productivity of laying hens at the age of 18 to 26 weeks with the use of BSF extract since it contains the highest protein compared to other treatments. Productivity variables show the best P3 treatment, each egg weight 57.17 g bird-1, daily egg production 90.88%, egg mass 2408.16 g bird-1 and ration conversion 2.0. The mean hematology of the blood showed the highest P3 treatment, each erythrocyte 2.91±0.13 x 106 mm-3, leucocytes 17.69±3.68 x 103 mm-3, hematocrit 27.8±1.14%, hemoglobin 9.96±1.12%, MCV 95.77±4.41 fl, MCHC 35.99±5.21%, MCH 34.38±4.53 pg, lymphocytes 61.2 ± 4.86%, heterophile 31.2±2%, monocytes 5±0.62% and ratio H/L 0.51±0.04.
EVALUASI KUALITAS WARNA IKAN KLOWN Amphiprion percula Lacepède 1802 TANGKAPAN ALAM DAN HASIL BUDIDAYA Sukarman Sukarman; Dewi Apri Astuti; Nur Bambang Priyo Utomo
Jurnal Riset Akuakultur Vol 12, No 3 (2017): (September 2017)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (189.316 KB) | DOI: 10.15578/jra.12.3.2017.231-239

Abstract

Kualitas warna ikan klown hasil budidaya lebih rendah dibandingkan tangkapan alam, hal ini dipengaruhi oleh berbagai faktor, namun belum ada data ilmiah sebagai dasar untuk melakukan perbaikan. Tujuan penelitian adalah menganalisis dan mengevaluasi kualitas warna ikan klown (Amphiprion percula) hasil tangkapan alam dibandingkan dengan hasil budidaya. Kualitas warna diukur pada dua zona: zona-I kulit berwarna oranye antara insang dengan band warna putih pada tengah badan dan zona-II adalah bagian kulit warna oranye antara band putih tengah badan dengan band warna putih pada pangkal ekor, dengan parameter nilai L* (lightness), a* (redness), b* (yellowness), C (chroma), H (Hue). Analisis total karotenoid (TC) dilakukan pada kulit kedua zona, sirip pektoral, sirip dorsal, sirip kaudal, dan serum darah. Analisis kromatografi lapis tipis (KLT) dilakukan pada kulit dan sirip untuk mengonfirmasi jenis karotenoid dalam kulit dan sirip. Data kualitas warna dianalisis menggunakan t-test, hubungan kualitas warna dengan TC dianalisis dengan regresi sederhana, dan analisis deskriptif untuk hasil KLT. Hasil penelitian menunjukkan bahwa kualitas warna ikan klown tangkapan alam lebih baik dibanding budidaya, didukung oleh tingginya total karotenoid pada kulit zona-I, kulit zona-II, sirip pektoral, sirip dorsal, sirip kaudal, dan serum darah berturut-turut 51,64; 51,24; 136,40; 124,37; 194,18 mg/kg; dan 2,2 mg/mL; pada ikan hasil budidaya berurut-turut 2,5; 3,5; 8,45; 10,01; 23,43 mg/kg; dan 0,8 mg/mL. Hasil KLT menunjukkan bahwa jenis karotenoid pada kulit dan sirip ikan klown adalah astaxanthin, serta satu jenis karotenoid diduga zeaxanthin. Berdasarkan hasil penelitian, maka perlu ditambahkan pigmen karotenoid, dan prekursor pigmen lainnya melalui pakan untuk ikan klown budidaya.The color quality of cultured clownfish is not as good as the wild one. However, it’s influenced by several factors. However, but there is not enough scientific data to be used as the basis for improvement. The purpose of this study was to analyze and evaluate the color quality difference between cultured and wild clown fish Amphiprion percula. Color qualities were measured in two zones: an orange-colored at zone-I was measured between gills and white band at the center of the body and an orange-colored at zone-II was measured between the center of white band and white band near the caudal peduncle. Parameters analyzed were L* (lightness), a* (redness), b* (yellowness), C (Chroma), H (Hue). Total carotenoid (TC) was analyzed on both zones-I and II, pectoral-fins, dorsal-fin, caudal-fin, and blood serum. Thin layer chromatography (TLC) was used to analyze the type of carotenoids in the skin and fin tissues. Color quality data was analyzed by T-test. Simple linier regression and descriptive analyses were used to analyzed the other parameters. The results showed that the color quality of wild clown fish was better than that of the cultured clown fish, indicated by high TC content in skin of zone-I, skin of zone-II, pectoral-fin, dorsal-fin, caudal-fin, and blood serum (51.64, 51.24, 136.40, 124.37, 194.18 mg/kg, and 2.2 mg/mL, respectively); and in cultured fish 2.5, 3.5, 8.45, 10.01, 23.43 mg/kg, and 0.8 mg/mL, respectively. TLC test results showed that carotenoid type in skin and fin of clownfish were astaxanthin, and one type of carotenoid was suspected as zeaxanthin. Based on the results of the study, it is necessary to add carotenoid pigments, especially astaxanthin or other precursor the feed to improve the color quality of cultured clown fish.
Co-Authors - Hernawati . Sumiati A Setiyono Achmad - Taher ACHMAD FARAJALLAH Achmad Taher Achmad Taher Achmad, Taher Agik Suprayogi Agita Rakhmawati Ainia Herminiati Akeme Cyril Njume Alma Agnia Alusyanti Primawati Anar, Muhammad Agung Firdhawansyah Andi Tarigan Andi Tarigan Andri Cahya Irawan Andri Cahya Irawan Anja Meryandini Anneke Anggraeni Anneke Anggraeni Annisa Annisa Annisa Annisa Anuraga Jayanegara Aris Purwanto Arismayanti Eka Armaji, Yone Asep Gunawan Asep Sudarman Aulia Evi Susanti Aulia Nurul Saputri Budi Setiawan Cece Sumantri Chairrusyuhur Arman Chusnul Choliq D Diapari D M Suci Didid Diapari Djokowoerjo Sastradipradja Dondin Sajuthi dortiana sijung, maria Dwierra Evvyernie DYAH PERWITASARI -FARAJALLAH Ekowati Handharyani Elizabeth Wina Elizabeth Wina Elmy Mariana Elmy mariana Elok Budi Retnani Endah Yuniarti Entang Iskandar Erika B. Laconi Evy Damayanthi f, Dilla Mareistia Fanny Rahmasari Daeli Fery Dwi Riptianingsih Firdus . Firkani, Rahmatiana Widi Fitriana, Eko Lela Hairani, Atikah Hanny Hafiar Harlystiarini Harlystiarini Harnowo Permadi Heri Ahmad Sukria Hernawati Hernawati I Gusti Bagus Wiksuana I Komang Gede Wiryawan I NENGAH BUDIARSA I wayan Teguh Wibawan Ida Wiryanti Imas Sukaesih Sitanggang Irawan Sugoro Irawan Sugoro Irawan Sugoro Irma Herawati Suparto Jakaria Jakaria Janah, Fatiah Finanur Jean-Baptise Menassol K Komalasari Kausar Daulay Kevin Alexander, Kevin Klaus Becker Kokom Komalasari Komang G. Wiryawan Kurnia Bagus Ariyanto L Khotijah Lilis Khotijah Lilis Khotijah Linar Zalinar Udin Lucia Cyrilla Eko Nugrohowati Luki Abdullah Mareistia Fassah, Dilla Mila Taniasari Mohamad Yamin Mohamad Yamin Mohammad Yamin Muhammad Agung Firdhawansyah Anar Muladno - nabawi, Soviro Nurul Lisa Nahid Ritcher Nahrowi Nahrowi Nanda Nadhifa Nuraini Nanis Nurhidayah Natsir Sandiah NE Maharani Nella Nurhazizah Novita Anggraeni Nur Bambang Priyo Utomo Nurina Rahmawati Pamungkas, Joko Panca Dewi Manu Hara Karti Pasetha Andre Pijoh, Deyv Pipih Suptijah Pursani paridjo Putri Sri Rahayu Qomariah Indira Nurul R. Iis Arifiantini Rangkuti, Farhan Ananda Rangkuti, Farhan Ananda Rangkuti Razak Achmad Hamzah Retno Wulansari Ria Oktarina Ridwan Raafi'udin Ridwan, Habibi Rima Shidqiyya Hidayati Martin Rimbawan , Rini Herlina Mulyono Riptianingsih, Fery Dwi Rita Mutia Rizki Palupi Ronnie Permana Ronny Rachman Noor Rudy Priyanto Sitanggang, Imas S. Siti Aslimah Siti Zubaidah Slamet Widodo Slamet Widodo sri murtini . Sri Rahayu SRI RAHAYU Sri Rahmatul Laila Sri Suharti Sri Supraptini Mansjoer Suharti, Sri Sukarman Sukarman SULISTIYANI SULISTIYANI Sulistiyani Sulistiyani SULISTIYANI SULISTIYANI Sulistiyani, Sulistiyani Sumiati . Sumiati . Sumiati . . Sumiati Sumiati SYAHRIAL SYAHRIAL Syamsul Arifin TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Teguh Wahyono Tiurma Pasaribu Triansyah, Irvan Walberto Sinaga Wasmen Manalu Waterman James O. Widya Hermana Wirdateti . Yuli Retnani