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Evaluasi Komposisi Tubuh dan Pemanfaatan Nutrien di Ambing Kambing Peranakan Etawah Laktasi yang Diberi Pakan Fermentasi Limbah Tempe: Evaluation of Body Composition and Nutrient Uptake on the Mammary Gland of Ettawah Crossbred Goat Lactation Fed by Fermented Tempeh Waste Dewi Apri Astuti; Erika B Laconi
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol. 17 No. 3 (2019): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1072.966 KB) | DOI: 10.29244/jintp.17.3.59-63

Abstract

The aim of this study was to evaluate the body composition, nutrient uptake in mammary gland and milk amino acid profile of lactating Etawah Crossbred goats fed by tempeh by product. Twelve lactating goats were randomly allocated into three groups which fed by ration containing concentrate (R1), concentrate plus 25% fresh tempeh waste (R2) and concentrate plus 25% fermented tempeh waste (R3). Tempeh waste was fermented by Aspergillus niger. Kinggrass was given 50% of the total ration for all groups. Urea space technique was used to measure body composition before and after the experiment was done. Milk production was calculated two times a day during two months after giving birth (postpartum). Total milk protein and amino acid, whey and casein were analyzed by kjeldahl method and amino acid analyzer respectively. Nutrient uptake in mammary gland was calculated base on Fick principles. Result showed that there was significantly different on body weight, but on body water, protein and fat had no significant difference between the treatments. The best milk production was found in fermented tempeh waste group. Concentration of glutamic acid was dominant than other essential amino acids in whole milk and casein but there were no significant difference between groups for those essential amino acid profile. It was concluded that fermented tempeh waste could substitute 50% of total concentrate and had the highest body weight, milk yield and nutrient uptake in the mammary gland of lactating Etawah Crossbred goats. Key words: body composition, fermentation, lactating PE goat, nutrient uptake, tempeh waste DAFTAR PUSTAKA Astuti DA & Sastradipradja D. 1998. Measurement of body composition using slaughter technique and urea-space in local sheep. Indonesian Journal of Veterinary Science. 3: 1-9 Astuti DA & Sastradipradja D. 1999. Evaluation of body composition using urea dilution and slaughter technique of growing Priangan sheep. Media Veteriner. 6 (3) : 5-9. Astuti DA, Sastradipradja D & Sutardi T. 2000. Nutrient balance and glucose metabolism of female growing, late pregnant and lactating ettawah crossbred goats. Asian-Australasian Journal of Animal Sciences. 13:8: 1068-1077 Astuti DA& Wina E. 2002. Protein balance and excreation of purine derivatives in urine of lactating etawah crossbred goats fed with tempe waste. Jurnal Peternakan dan Veteriner. 7(3) : 162-166 Astuti DA, Baba AS & Wibawan IWT. 2011. Rumen fermentation, blood metabolites, and performance of sheep fed tropical browse plants. Media Peternakan. 34 (3) : 201-206 Astuti DA & Sudarman A. 2015. Status fisiologi, profil darah dan komposisi tubuh domba yang diberi minyak lemuru tersaponifikasi dan disalut dengan herbal. Buletin Peternakan. 39 (2): 116-122. Badan Pengendalian Pengendalian Dampak Lingkungan Daerah. 2000. Laporan Pertanggungjawaban Pembangunan Instalasi Pengolahan Limbah Cair Industri Tahu Tempe PRIMKOPTI Ngoto Yogyakarta. Yogyakarta (ID) : Setwilda Propinsi DIY Bishop JM, Hill, DJ, & Hosking CS. 1990. Goat milk does not suppress the immune system. Journal of Pediatrics. 116: 862-867 Bruhn JC, FST & Davis CA. 1999. Dairy goat milk composition. https://drinc.ucdavis.edu/goat-dairy-foods/dairy-goat-milk-composition Cant JP, DePeters EJ & Baldwin RL, 1993. Mammary amino acid utilization in dairy cows fed fat and its relationship to milk protein depression. Journal of Dairy Science. 76 (3) :762-774 Chaiyabutr N, Komolvanich S, Preuksagorn S & Chanpongsang S. 2000. Comparative studies on the utilization of glucose in the mammary gland of crossbred holstein cattle feeding on different types of roughage during different stages of lactation. Asian-Australasian Journal of Animal Sciences. 13 (3): 334 – 347. Laconi EB, & Jayanegara A. 2015. Improving nutritional quality of cocoa pod (theobroma cacao) through chemical and biological treatments for ruminant feeding: in vitro and in vivo evaluation. Asian-Australasian Journal of Animal Sciences Open Access. http://dx.doi.org/10.5713/ajas.13.0798. Lehninger AL. 1982. Principles of Biochemistry. New York (US): Woth Publisher, Inc. NRC. 1990. Nutrient Requirement of Goat. Washington (US): National Academy of Science. Panaretto BA & Till A.R.. 1963. Body compositition in vivo. II. The composition of mature goats and its relationship to the antypyrene, tritiated water and acetyl-4-aminoantipyrene spaces. Australian Journal of Agricultural Research. 14 (6): 926 – 943 Park YW. 1991. Goat milk as a substitute for those who are lactose intolerant. Journal of Dairy Science 74:3326-3333 Riis PM. 1983. Dynamic Biochemistry of Animal Production. New York (US): Elsevier Science Rovanis. 1995. Letters in applied microbiology. Journal of Milk and Food Technology. 20 (3): 164-167 Steel RGD & Torrie JH, 1993. Principles and Procedures of Statistics. New York (US): McGraw Hill Book Co. Inc. Shapiro BA, Harrison RA & Walton JR, 1982. Clinical Application of Blood Gas. 3rd ed. London (UK): Book Medical Publishers, Inc. Waghorn GC & Baldwin, RL. 1984. Model of metabolic flux within mammary gland of the lactating cows. Journal of Dairy Science. 67: 531-544
Kecernaan Nutrien Domba Fase Akhir Kebuntingan yang Diberi Ransum Flushing dengan Frekuensi yang Berbeda: Nutrient Digestibility of Local Ewes in Late Pregnancy Phase Fed by Flushing Ration with Different Feeding Frequency Aulia Nurul Saputri; Dewi Apri Astuti; Dilla Mareistia Fassah
Jurnal Ilmu Nutrisi dan Teknologi Pakan (Nutrition and Feed Technology Journal) Vol. 20 No. 1 (2022): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.20.1.14-18

Abstract

The aim of this research was to evaluate the nutrient digestibility of late pregnancy local ewes treated with flushing ration and different feeding frequency. Twelve local ewes in the late pregnancy phase (BW 49.96 ± 9.67 kg) were divided on a Randomized Block Design with 4 treatments and 3 replications. The treatments were P1 (without flushing), P2 (flushing at the before and after mating), P3 (flushing at the before and after mating, and middle of pregnancy), and P4 (flushing at the before and after mating, middle, and late of pregnancy). Flushing concentrate containing 2% coconut oil and 5% lemuru fish oil. The variables observed were nutrient consumption and nutrient digestibility. The data were analyzed by using ANOVA, the data with significant differences in the treatment effects were further tested using Duncan Test. The result showed that the treatments significantly affected (p<0.01) consumption of crude fat. Giving the flushing ration at the before and after mating, and mid pregnancy significantly increased (p<0.05) digestibility of dry matter, crude protein, crude fiber, and N-free extract. It can be concluded that the feeding frequency of flushing affects the consumption and digestibility of local sheep at the late stages of pregnancy. Giving flushing ration at the before and after mating, and middle of pregnancy showed the best digestibility of dry matter, crude protein, crude fiber, and N-free extract. Key words: flushing, lemuru fish oil, local ewes, nutrient digestibility
Single Nucleotide Polymorphism within the Promoter Region of Cynomolgus Monkey LDLR Gene Achmad, Taher; Solihin, Dedy Duryadi; Sulistiyani, Sulistiyani; Astuti, Dewi Apri; Sajuthi, Dondin
Makara Journal of Science Vol. 22, No. 1
Publisher : UI Scholars Hub

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Abstract

The cynomolgus monkey (Macaca fascicularis) is one of the most common nonhuman primates used as an animal model in biomedical research related to atherosclerosis. However, little is known about genetic variation in the low density lipoprotein receptor (LDLR) gene, its effect on cholesterol levels, and associated risks of atherosclerosis. There-fore this study aimed to identify genetic polymorphisms, namely single nucleotide polymorphisms (SNPs), within the promoter region of LDLR and their relationship with animal responsiveness to hypercholesterolemia due to an atherogenic diet. In this research, SNPs were studied using DNA isolated from22 cynomolgus monkeys obtained from aprevious study, consisting of two hyporesponders, 19 hyperresponders, and one extremecase. The result showed that two SNPs existed in the promoter region, namelyg.−169T>C and g.−265G>A. SNP g.−265G>A showed linkages with extreme responsiveness and can be used as a potential genetic marker for extreme animals. The result of this study has extended our knowledge of polymorphism in the LDLR geneand its use intheselection of cynomolgus monkeys as animal models in research on hypercholesterolemia and atherosclerosis.
Feeding Behavior of Tonkean Macaques (Macaca tonkeana) in Schmutzer Primates Center and Ragunan Zoo, Jakarta Riptianingsih, Fery Dwi; Farajallah, Dyah Perwitasari; Astuti, Dewi Apri
Makara Journal of Science Vol. 19, No. 2
Publisher : UI Scholars Hub

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Abstract

Tonkean macaques are one of seven endemic macaque species on Sulawesi Island. Feeding management in captivity should pay attention to the quality, palatability, and feeding behavior patterns of animals. The goal of this study was to compare the feeding behavior of two social groups of Tonkean macaques at Schmutzer Primates Center (SPC) and Ragunan Zoo (RZ) with different captive management, which was expected to affect feeding behavior. Ad libitum sampling was used to observe daily behavior and hierarchy, while focal animal sampling was used to observe feeding behavior and feed preference. Data were collected from September 2013 until March 2014 with a total of 495 hours of observations. There were significant differences between the daily behavior of two groups of Tonkean macaques. Resting behavior was dominant in RZ group with non-enrichment feed cage, while feeding behavior was more common in the SPC group with an enrichment feed cage. The SPC group spent most of their feeding time in searching for feed, while choosing, carrying and refusing were greater in the RZ group. Both Tonkean macaque groups showed individual dominance in their feeding behavior. Provisioned feed in both locations had different diversity and preference values. The selection of feed required was based on preference values with attention to Tonkean macaques’ feed in nature. Cage construction, such as the SPC cage, was able to reduce abnormal behavior exhibited by individuals.
Analysis of Strategy for Developing Beef Cattle Production on a Smallholder Farming Basis in Musi Banyuasin Regency South Sumatra Aulia Evi Susanti; Rudy Priyanto -; Muladno - -; Dewi Apri Astuti; Lucia Cyrilla Eko Nugrohowati
Buletin Peternakan Vol 46, No 4 (2022): BULETIN PETERNAKAN VOL. 46 (4) NOVEMBER 2022
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v46i4.73137

Abstract

The increased beef cattle production at traditional farms is a strategy to achieve meat self-sufficiency in 2026. This is accomplished through various parties, including the government, academics, researchers, the private sector, and farmers as the main actors. Therefore, this study aimed to determine and analyze criteria, performance criteria, strategies, and alternatives for sustainable beef cattle production in traditional farms based on the opinion of experts. The research location was School for Smallholder Community (SPR) Maju Bersama, Sungai Lilin Sub-District, Musi Banyuasin Regency, South Sumatra Province, Indonesia. Primary data were collected through FGD (focus group discussion) and interview with expert stakeholders, including farmers, policymakers, entrepreneurs, academics, and research analyst. They were analyzed using the Analytical Hierarchy Process (AHP) with R studio version 4.1.2. The result showed that Musi Banyuasin Regency has a natural resource potential for supporting beef cattle production with an institutional collaborative strategy. The interaction between the production factors showed that the feeding criteria were at the highest level, with 32.05%. Considering the performance criteria, business sustainability was essential at 35.70% and should be achieved by adopting a corporate strategy of 85.52%, implemented through alternative business management at 61.40%.
Profil Hematologi Induk Domba dengan Pemberian Pakan Flushing Berbeda: Hematologic Profile of The Ewes by Giving Different Flushing Feed Dewi Apri Astuti; NE Maharani; D Diapari; L Khotijah; K Komalasari
Jurnal Ilmu Nutrisi dan Teknologi Pakan (Nutrition and Feed Technology Journal) Vol. 20 No. 2 (2022): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.20.2.44-50

Abstract

This study aimed to analyze the hematological profiles of late pragnant sheep fed by flushing ration. This research used twelve multiparous lactating ewes of thin tale local sheep, with the average body weight of 29,75 ± 6,20 kg head-1 and placed in individual sheep pen. This research used a randomized block design (RBD) with 4x3 that was grouped based on body weight of ewes. The treatments consisted of R1 = treatment without flushing, R2 = flushing treatments before and after matting, R3 = flushing treatment same with R2 plus during in the middle of pregnancy, R4 = flushing treatment same with R3 plus during at the end of pregnancy. Ratio forages and concentrate in the ration were 30:70% based on dry matter basis. The observed variables were hematological profiles and nutrient intake. The data were analyzed using ANOVA and the significance of treatments was continued to have the analysis through Duncan test. The results showed that the treatments did not affect significantly to the hematological profiles nor the nutrients intake, except to the amount of white blood cell. The conclusion of this research was the different flushing management for the late pregnancy ewes did not give a significant effect on hematological profiles and nutrient consumption. Key words: flushing, hematology, local thin tale sheep ewes, mating
PREGNANT AND LACTATING Macaca nigra: BEHAVIOR AND FOOD SELECTION Dyah Perwitasari-Farajallah; Arismayanti Eka; Qomariah Indira Nurul; Pasetha Andre; Astuti Dewi Apri; Waterman James O.
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 29 No. 2 (2022): BIOTROPIA Vol. 29 No. 2 Agustus 2022
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2022.29.2.1687

Abstract

Pregnancy and lactation are reproductive phases that require large amounts of energy. Females in the reproductive period need good quality and quantity of food to provide nutrition for the fetus, milk production and child care. The mother will adapt to changes in behavior patterns and food type to meet these needs. The influence of parity and environmental conditions can affect the behavior patterns of females. During pregnancy, the Macaca nigra is known to have different proportion of activities in each period, while the behavior during the lactation phase in each mester is unknown. Therefore, this study aimed to analyze the behavior patterns in each mester and the food selection of Macaca nigra during the pregnancy and lactation phases, as well as the influence of female parity and environmental toward the behavior patterns. There were 39 females Macaca nigra observed from two groups from August 2018 to July 2019. An instantaneous focal sampling method was performed to observe females’ daily activities, continuous focal sampling to monitor food types and a selectivity index to analyze food type preferences. The results showed that the female Macaca nigra pattern was influenced by the reproductive phase, female parity and environmental conditions. Females at the end of the pregnancy and lactation phases had a high proportion of feeding and eat more arthropods. Primiparous females mostly performed resting activities. Food preference was influenced by reproductive factors and food availability. The choice of fruit could be affected by fruit availability, and their favorite food was D. mangiferum and Euginia sp.  
Karakteristik Fermentasi Rumen Domba Secara In Vitro dengan Pemberian Maggot Black Soldier Fly yang Dipelihara dengan Ampas Teh dan Ampas Sagu: Characteristics of In-Vitro Sheep Rumen Fermentation of Black Soldier Fly Maggot Reared on Tea Waste and Sago Pulp Dilla Mareistia Fassah; Nella Nurhazizah; Dewi Apri Astuti; Lilis Khotijah
Jurnal Ilmu Nutrisi dan Teknologi Pakan (Nutrition and Feed Technology Journal) Vol. 20 No. 3 (2022): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.20.3.111-116

Abstract

The objective of this study was to evaluate the chemical composition of Black Soldier Fly (BSF) maggot grown in tea waste or sago pulp medium and their effects on fermentability and digestibility (in vitro). A randomized block design with 3 dietary treatments and 4 groups of rumen source from different sheep was used in this study. Dietary treatments consisted of P1 = soybean meal protein ration, P2 = BSF maggot meal protein ration grown in tea waste, P3 = BSF maggot meal protein ration grown in sago pulp. The data were analysed using ANOVA followed by Duncan's test. The results show that the NH3 concentration was higher (p<0.05) in treatment containing maggot meal protein grown in tea waste than others. However, each treatment did not have a significant effect on pH, total VFA, dry matter digestibility and organic matter digestibility in vitro. In conclusion, BSF maggot grown in tea waste and sago pulp media can replace soybean meal as a protein source in the ration without any negative effects on in vitro fermentability and digestibility. Key words: black soldier fly, digestibility, fermentability, in vitro, rumen microbes
In vitro study: Soybean meal substitution with maggot on sheep digestibility and rumen fermentation Lilis Khotijah; Putri Sri Rahayu; Dewi Apri Astuti; Rima Shidqiyya Hidayati Martin
Jurnal Ilmu Peternakan Terapan Vol 6 No 2 (2023): Jurnal Ilmu Peternakan Terapan
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/jipt.v6i2.3771

Abstract

The in vitro study aimed to evaluate the utilization of maggot flour from selected substrates as a substitute for soybean meal in sheep rations on digestibility and rumen fermentation in vitro. Maggot were reared using three different substrates i.e., sheep feces, household waste and bread waste. The BSF maggot product with feces substrate showed the highest protein was used in this study. The treatments in this study were T1=100% soybean meal, T2=50% soybean meal + 50% maggot flour, T3=100% maggot flour. In vitro rumen fermentation and digestibility were conducted and the parameters were pH, NH3, volatile fatty acid (VFA), dry matter digestibility (DMD) and organic matter digestibility (OMD). Maggot rearing data were presented descriptively while the in vitro data were analyzed using analysis of variance. The result showed that the treatments had no significant effect (P>0.05) on pH, VFA, DMD and OMD, but replacement of soybean meal (100% maggot flour) affected NH3 production. The utilization of maggot as a substitute for soybean meal in sheep ration did not have a negative impact on in vitro fermentability and rumen digestibility.
Kajian Pemenuhan Kebutuhan Nutrien dan Energi pada Kuda Bunting dan Laktasi di Peternakan Kuda di Bogor: Study of Nutrient Requirement and Feeding of Pregnant and Lactating Mares in Several Stable in Bogor Alma Agnia; Dewi Apri Astuti; Yuli Retnani
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol. 21 No. 1 (2023): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.21.1.21-28

Abstract

This study aimed to determine the nutrient and energy requirement in pregnant and lactating mares. This study used an observation methodology and it was conducted on eight stables with 11 pregnant mares and 16 lactating mares. Observed variables included mare profile (estimated body weight, horse type, physiological condition (pregnancy or lactation), feeding (type of feed, amount of feed, nutrient content, total feed consumption) then compared with nutrient requirement based on NRC (2007). Data on feed consumption were collected based on different physiological conditions (pregnancy and lactation) and body weight. Given feed was analysed by proximate analysis. The results of this study showed that the nutrient consumption of pregnant and lactating mares on one stable did not meet the overall requirement, three stables had not met the energy requirement and two stables had higher ratio of Ca:P to P. Key words: lactating mare, mare, mare feed, nutrient, pregnant mare
Co-Authors - Hernawati . Sumiati A Setiyono Achmad - Taher ACHMAD FARAJALLAH Achmad Taher Achmad Taher Achmad, Taher Agik Suprayogi Agita Rakhmawati Ainia Herminiati Akeme Cyril Njume Alma Agnia Alusyanti Primawati Anar, Muhammad Agung Firdhawansyah Andi Tarigan Andi Tarigan Andri Cahya Irawan Andri Cahya Irawan Anja Meryandini Anneke Anggraeni Anneke Anggraeni Annisa Annisa Annisa Annisa Anuraga Jayanegara Aris Purwanto Arismayanti Eka Armaji, Yone Asep Gunawan Asep Sudarman Aulia Evi Susanti Aulia Nurul Saputri Budi Setiawan Cece Sumantri Chairrusyuhur Arman Chusnul Choliq D Diapari D M Suci Didid Diapari Djokowoerjo Sastradipradja Dondin Sajuthi dortiana sijung, maria Dwierra Evvyernie DYAH PERWITASARI -FARAJALLAH Ekowati Handharyani Elizabeth Wina Elizabeth Wina Elmy mariana Elmy Mariana Elok Budi Retnani Endah Yuniarti Entang Iskandar Erika B. Laconi Evy Damayanthi f, Dilla Mareistia Fanny Rahmasari Daeli Fery Dwi Riptianingsih Firdus . Firkani, Rahmatiana Widi Fitriana, Eko Lela Hairani, Atikah Hanny Hafiar Harlystiarini Harlystiarini Harnowo Permadi Heri Ahmad Sukria Hernawati Hernawati I Gusti Bagus Wiksuana I Komang Gede Wiryawan I NENGAH BUDIARSA I wayan Teguh Wibawan Ida Wiryanti Imas Sukaesih Sitanggang Irawan Sugoro Irawan Sugoro Irawan Sugoro Irma Herawati Suparto Jakaria Jakaria Janah, Fatiah Finanur Jean-Baptise Menassol K Komalasari Kausar Daulay Kevin Alexander, Kevin Klaus Becker Kokom Komalasari Komang G. Wiryawan Kurnia Bagus Ariyanto L Khotijah Lilis Khotijah Lilis Khotijah Linar Zalinar Udin Lucia Cyrilla Eko Nugrohowati Luki Abdullah Mareistia Fassah, Dilla Mila Taniasari Mohamad Yamin Mohamad Yamin Mohammad Yamin Muhammad Agung Firdhawansyah Anar Muladno - nabawi, Soviro Nurul Lisa Nahid Ritcher Nahrowi Nahrowi Nanda Nadhifa Nuraini Nanis Nurhidayah Natsir Sandiah NE Maharani Nella Nurhazizah Novita Anggraeni Nur Bambang Priyo Utomo Nurina Rahmawati Pamungkas, Joko Panca Dewi Manu Hara Karti Pasetha Andre Pijoh, Deyv Pipih Suptijah Pursani paridjo Putri Sri Rahayu Qomariah Indira Nurul R. Iis Arifiantini Rangkuti, Farhan Ananda Rangkuti, Farhan Ananda Rangkuti Razak Achmad Hamzah Retno Wulansari Ria Oktarina Ridwan Raafi'udin Ridwan, Habibi Rima Shidqiyya Hidayati Martin Rimbawan , Rini Herlina Mulyono Riptianingsih, Fery Dwi Rita Mutia Rizki Palupi Ronnie Permana Ronny Rachman Noor Rudy Priyanto Sitanggang, Imas S. Siti Aslimah Siti Zubaidah Slamet Widodo Slamet Widodo sri murtini . Sri Rahayu SRI RAHAYU Sri Rahmatul Laila Sri Suharti Sri Supraptini Mansjoer Suharti, Sri Sukarman Sukarman Sulistiyani Sulistiyani SULISTIYANI SULISTIYANI SULISTIYANI SULISTIYANI Sulistiyani, Sulistiyani Sumiati . Sumiati . Sumiati . . Sumiati Sumiati SYAHRIAL SYAHRIAL Syamsul Arifin TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Teguh Wahyono Tiurma Pasaribu Triansyah, Irvan Walberto Sinaga Wasmen Manalu Waterman James O. Widya Hermana Wirdateti . Yuli Retnani