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Struktur 3D Protein Struktural VP1 pada Enterovirus A71 Menggunakan Swiss-Model Suprianto; Made Budiarsa; Fatmah Dhafir
BIOEDUSCIENCE Vol 4 No 1 (2020): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (642.404 KB) | DOI: 10.29405/j.bes/4137-474353

Abstract

Background: Protein struktural VP1 berperan sebagai pemain kunci dalam patogenesis, memiliki keunikan yang cukup menarik untuk dikaji dengan mempelajari sifat dan fungsi protein struktural VP1. Penelitian ini bertujuan untuk memprediksi struktur tiga dimensi protein VP1 pada EV-A71. Metode: Protein target diperoleh dari server UniProt dengan kode akses A0A097EV89 menggunakan template 4cey.1.A (PDB ID) dianalisis secara in silico melalui metode homologi menggunakan server SWISS-MODEL. Hasil: analisis penelitian menunjukkan protein target dan template memiliki identity 95,29 % dan tersusun dari 297 asam amino dengan nilai QMEAN -2,15. Protein struktural VP1 pada Ramachandran Plots memiliki struktur stabil, residu non-glisin pada daerah outlier hanya berkisar 0,34 % (A53 ALA) dengan Nilai rotamer outliers 1,61 %. Kesimpulan: Model struktur tiga dimensi protein yang diteliti memiliki struktur stabil dan informasi yang didapatkan berguna untuk penelitian lebih lanjut dalam pengembangan vaksin penyakit yang disebabkan oleh EV-A71.
Keanekaragaman dan Pola Penyebaran Makrozoobentos di Sungai Pondo dan Pemanfaatannya sebagai Sumber Belajar Ayu Rifka Ramadani; Fatmah Dhafir; Masrianih Masrianih; Syech Zainal
Media Eksakta Vol 17 No 1 (2021)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (381.815 KB) | DOI: 10.22487/me.v17i1.812

Abstract

The Pondo River is one of the rivers in Central Sulawesi which crosses the Poboya, Lasoani, Tanamodindi and Talise villages. Administratively, it is located in Mantikulore District, Poboya Village. This study aims to determine the level of diversity and distribution patterns of macrozoobenthos in the Pondo River and its use as a learning resource. This research is a descriptive study that uses a random sampling method, with surbernet as a means of taking samples. To determine the level of diversity using the Shannon-Winner diversity index formula, to determine the distribution pattern using the Morista distribution index. The results showed the diversity of macrozoobenthos in the Pondo River, namely: H '= 1.36 moderate diversity. The distribution pattern of macrozoobenthos, namely: The pattern of distribution in groups (clustered) and uniform (regular), of the 10 macrozoobenthos found in the species Hydropsyche sp. has a clustered distribution pattern (clustered). Based on the results of the validation test, this research is suitable to be used as a learning resource in the form of a pocket book with a feasibility percentage of 82.1%.
Kandungan Mikroplastik pada Saluran Pencernaan Ikan Katombo (Rastrellinger kanagurta) dari Teluk Palu dan Pemanfaatannya sebagai Media Pembelajaran Moh Dhuhri Surya Wirawan; Fatmah Dhafir; I Made Budiarsa; Gamar Binti Non Shamdas
Media Eksakta Vol 17 No 2 (2021)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (319.667 KB) | DOI: 10.22487/me.v17i2.1017

Abstract

Microplastics are plastic pieces smaller than 5 mm. This study aims to describe the mikroplastic content in the digestive tract of Mackerel fish (Rastrellinger kanagurta) arising from the Palu Bay, and its use as a learning media. Samples in this research are some 10 % of the catch fishermen. Technique data collection was carried out using a technique analysis of the stomach contents. Result of the research are that abundance highest on station I with 2,2 particles, station II 0,83 particles, and in stations III 1,5 particles.  Validated of video by team validator obtained to 76.86 %, video can be described as learning so that it can be used as a Learning media.
Tingkah Laku Bertelur Burung Maleo (Macrochepalon maleo S. Muller) di Taman Nasional Lore Lindu (TNLL) Blok Saluki Fatmah Dhafir; Bustamin Bustamin; Isnainar Isnainar; Manap Trianto
Media Eksakta Vol 18 No 1 (2022)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (384.18 KB) | DOI: 10.22487/me.v18i1.1712

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Knowledge of behavior is very important to maintain the habitat of the maleo as an endemic animal to Sulawesi that must be preserved. Research takes place in March-December 2021. The research location is in the Saluki Lore Lindu National Park, Central Sulawesi Province. The study took place in March-December 2021. The findings targeted in the study are: (1) Information on egg-laying behavior, (2). When laying eggs, (3) After laying eggs, (4) Determining the length of time for laying eggs, (5) Physical characteristics of the nest and vegetation. The research method used the survey method. Observations of maleo bird activity were carried out every day for 3 months in the egg-laying season from May to July. The results showed that the physical condition of the average nest depth was 67.78 cm, the average nest width was 52.78 cm, the average nest temperature was 33.44°C, the nest humidity was 76.11% and, the soil acidity (pH) with an average of 6.35. The vegetation found at the nesting site consisted of 18 species. The maleo maleo bird's egg-laying behavior activities are playing, snooping (observation), and making a trick hole. There were four activities of female maleo bird laying behavior, namely playing, digging holes for laying eggs, lurking, making trick holes and laying eggs. The duration of time needed by male maleo birds when playing, snooping, making holes, until the female finishes laying eggs, which takes 92 minutes. While the female takes 84 minutes.
Kelimpahan Jenis Burung di Areal Kampus Universitas Tadulako dan Pemanfaatannya sebagai Media Pembelajaran Musdayanti Musdayanti; Fatmah Dhafir; Syech Zainal
Media Eksakta Vol 18 No 1 (2022)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (270.378 KB) | DOI: 10.22487/me.v18i1.1978

Abstract

This study aims to describe the abundance of bird species in campus area of the Tadulako University and the results of this study is used as a Pocket Book about bird species as a medium for learning biology. The method used is the IPA (Indices Ponctual Abundance) method with a purposive sampling technique. The results of this study found 25 species of birds including: Lanius sahach, Passer montanus, Pycnonotus aurigaster, Lalage sueurii, Hirundo rustica, Saxicola caprata, Lonchura pallida, Corvus enca, Gerygone sulphurea, Acridotheres javanicus, Hirundo rustica, Saxicola caprata, Lonchura pallida, Corvus enca, Gerygone sulphurea, Acridotheres javanicus, Necterinia jugulopus, Stylus , Ptilinopus melanospilus, Streptopelia chinensis, Geopelia striata, Centropus bengaleansis, Cacomantis merulinus, Merops philippinus, Todirhampus chloris, Collocalia esculenta, Turnix suscitatur, Gallirallus torquatus, Falco moluccensis, Cystola moluncensis, Cisticola juncid. 8 orders, 21 families, and 25 genera. and for the dominant abundance level there are 5 types namely Passer montanus, Pycnonotus aurigaster, Hirundo rustica, Lonchura pallida, and Zosterops palpebrosus. Evaluation of media by content experts by 78%, design experts by 84%, and media experts by 67.14%. Validation from students as many as 15 people amounted to 87.25% thus the pocket book media was declared fit for use as a learning medium.
Pollen collected by stingless bees Tetragonula laeviceps (Smith, 1857) (Hymenoptera: Apidae: Meliponini) from Central Sulawesi Masrianih Masrianih; Fatmah Dhafir; Manap Trianto
Jurnal Biologi Tropis Vol. 22 No. 3 (2022): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v22i3.3885

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The diversity of pollinating insects is used as conservation management. Pollen is gametophyte from male which can give characteristics to its offspring. The pollen carried by bees can be used as an identification of feed favored by bees. This study aims to identity pollen derived from the legs of stingless bees based on the color morphological characteristics of the pollen. The methods include color identification of pollen collected by stingless bees, pollen extraction, acetolysis, and pollen identification. Pollen with the same species at the study site has a color that is not much different. Stingless bees take pollen based on the size and distance of the plant to the hive. Smell and color as a secondary binder for stingless bees. Different plant pollen species can also be influenced by the protein requirements of each stingless bee colony. It can recognize the presence of flowering plants aroud the hive. The size of pollen can affect the bees in taking plant pollen.  
Studi In Silico: Hasil BLAST Gen Clock pada Megapodiidae I Made Budiarsa; Fatmah Dhafir; Suprianto Suprianto
JRST (Jurnal Riset Sains dan Teknologi) Volume 6 No. 1 Maret 2022: JRST
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (929.116 KB) | DOI: 10.30595/jrst.v6i1.10827

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Gen clock merupakan salah satu gen yang berperan penting dalam mengontrol ritme harian dan perilaku hewan. Beberapa jenis burung memiliki kerentanan terhadap kondisi fisik lingkungan yang berubah-ubah, seperti kelompok burung dari famili megapodiidae. Urutan nukleotida gen target diperoleh dari beberapa spesies Megapodiidae di GenBank dengan kode akses KY762758.1 (Megapodius eremite) dan KY762668.1 (Alectura lathami). Penelitian ini menggunakan metode komputasi, semua data yang diperoleh dihasilkan dari analisis secara  in silico urutan nukleotida gen target. Tujuan penelitian ini adalah untuk menganalisis beberapa informasi penting, terkait filogenetik dan perkembangan database gen clock pada Megapodiidae di GenBank. Urutan nukleotida gen clock pada Megapodius eremite mempunyai panjang  530 bp, nilai identitas 100 % (530/530), Gaps 0 % (0/530), Strand Plus/Plus, Expect 0.0 dan Skor 961 bits (520). Urutan nukleotida gen clock pada Alectura lathami mempunyai panjang 522 bp, nilai identitas 97 % (514/530), Gaps 1 % (8/530), Strand Plus/Plus, Expect 0.0 dan Skor 894 bits (484). Hasil Run BLAST menggambarkan bahwa informasi sequence gen clock pada Megapodiidae masih sangat jarang dikaji dan diteliti, sehingga informasi dasar terkait gen clock dari sebagian besar spesies kelompok Megapodiidae belum terdaftar di GenBank sehingga hal ini sangat mempengaruhi studi lanjut terkait gen clock pada Megapodiidae yang sangat dibutuhkan untuk keperluan riset-riset selanjutnya dibidang molekuler atau bahkan konservasi.
Variasi Morfometrik Tetragonula laeviceps di Berbagai Habitat di Provinsi Sulawesi Tengah I Made Budiarsa; Fatmah Dhafir; Manap Trianto
BIOEDUSCIENCE Vol 6 No 2 (2022): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (301.233 KB) | DOI: 10.22236/j.bes/625062

Abstract

Background: Habitat is a place for living things to grow and develop. Differences in habitat in an area can affect the size of an organism. This study aims to determine the morphometric variations of Tetragonula laeviceps found in urban and forest habitats in Central Sulawesi Province. Methods: This research was conducted with roaming technique. Morphometric characters observed were 35 characters of stingless bee and analyzed using Microsoft Excel software. Results: The results of this study indicated that the morphometric size variation of T. laeviceps found in forest habitats (3.58 mm) is greater than that of wild bee in urban habitats (3.05 mm). Conclusions: Different habitat characteristics can affect the morphometry variations of the bee, especially in T. laeviceps species. The bee's morphometry body size is greater than in the forest habitat, because in that habitat there are environmental temperatures and types of plant sources of nectar which are more supportive for bee life compared to urban habitats.
Morphology, Morphometry, and Nest Structure of Tetragonula biroi (Hymenoptera: Meliponini) In Central Sulawesi Sitti Nur Fadhilah; I Made Budiarsa; Masrianih; Fatmah Dhafir; Achmad Ramadhan; Manap Trianto
Jurnal Biologi Tropis Vol. 23 No. 3 (2023): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i3.4985

Abstract

Stingless bees are social bees that live in colonies. Indonesia has a variety of stingless bee species that can be identified based on morphological, morphometric and nest structure characters. This study aims to describe the morphological, morphometric and nest structure characteristics of Stingless bee Tetragonula biroi from Sigi Regency. This study used the roaming method to find samples in nature, with a purposive sampling technique. Obtained morphological, morphometry and nest structure data were analyzed using the Principal Component Analysis (PCA) method with PAST4 software. The morphological characters of the worker bee Tetragonula biroi have a dominant black body, blackish brown abdomen, antennae with 11 flagellomeres, black thorax, black hair standing on the mesoscutum and mesoscutellum, brownish black mandibles, with 5 hamuli. The most dominant character in the formation of seven groups of stingless bees in this study, namely Hamuli Number (HN), Fore Wing Length (FWL), and Length of Forewing Including Tegula (WL1). The nest entrance hole of T. biroi is elliptical in shape with a width of 7.1 cm and a height of 2.8 cm and is light brown in color. The internal structure of the nest of T. biroi consists of honey cells, pollen cells and stem cells. The existence of size differences in the same specimen and from different places, can be influenced by several environmental factors and is also a form of morphological adaptation.
Characteristics Morphology and Morphometry Lepidotrigona terminata (Hymenoptera: Meliponini) in Central Sulawesi Nur Hikmah; I Made Budiarsa; Fatmah Dhafir; Mursito S. Bialangi; Masrianih; Manap Trianto
Jurnal Biologi Tropis Vol. 23 No. 3 (2023): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i3.5005

Abstract

Stingless bees are eusocial bees belonging to the Apidae family and the Meliponini subfamily. This study aims to describe the morphological and morphometric and nest structure characteristics of Stingless bee Lepidotrigona terminata from Parigi Moutong Regency. This study used the roaming method with a purposive sampling technique. Obtained morphological and morphometry data were analyzed using the Principal Component Analysis (PCA) method with PAST4 software. The morphological characters of Lepidotrigona terminata had a dominant black body, on the thorax, black scutum and scutellum covered with brown hair and yellow packaging on the edges. The tegula is brown, the tibia on the legs is black, the hind tibia is slightly hairy (plumose), antennae with 11 flagellomeres, the number of hamuli is 8. The most dominant character in the formation of seven groups of stingless bees in this study were Hamuli Number (HN), Fore Wing Length (FWL), and Length of Forewing Including Tegula (WL1). There are differences in character size compared to the same or different species in other areas.
Co-Authors Abd Hakim Laenggeng, Abd Hakim Abd Rauf Abd. Hakim Laenggeng, Abd. Hakim Abd. Rauf Abdul Hakim Achmad - Ramadhan, Achmad - Achmad Ramadhan Achmad Ramadhan Achmad, Mestawaty As. Agustin Tiwow Amalia Buntu Amiruddin Kasim Amran Rede Amran Rede Andi Mamas Andi Tanra Tellu Annisa, Rezky Artha, Fandy Ashari, Abdul Ayu Rifka Ramadani Burhan Burhan, Burhan Bustamin Bustamin Bustamin Bustamin Bustamin Bustamin D, Mutiara Datubaringan, Jein David Pebriyanto Tulak Dawa, Linda M Delima, Nur Dewi Tureni Erna, Ni Kadek Fadhilah Fadhilah Faradila, Ratna Febriawan, Aan Firga Feliana, Firga Gamar Binti Non Shamdas Hadijah S. Pago Hj. Musdalifah Nurdin I Ketut Suada I Made Budiarsa I Nengah Kundera Illah, Ininna Nur Isnainar Isnainar Isninar, Isnainar Kaltisa, Kaltisa Laksono Trisnantoro Latompai, Nismawati I. Lilies Lusiana - - Manap Trianto Manap Trianto MARIA BINTANG Maria Ulpa Djuanda Masriani . Masrianih Mestawaty As. Achmad Mestawaty Mestawaty Minarni Rama Jura Moh Dhuhri Surya Wirawan Mohammad Jamhari Murnadi, Ni Kade Mursito S. Bialangi Musdayanti Musdayanti Najma AR. Talamoa Nismawati I. Latompai Novriadi Novriadi Nur Hikmah Nursam Nursam Nurwahda, Nurwahda Permatasari, Ni Kadek Dewi Puspitasari, Amira Putri, Adelia Putri, Aticha Anata Rafiqa RAFIQA RAFIQA, RAFIQA Rahmadhani, Lili Ratnawathi, Ni Wayan Raya Agni Rezkiana, Sry Ayu Safitri, Gusti Ayu Kadek Yunita Samsurizal, M. Sulaeman Silvia Agustin Sitti Nur Fadhilah Suardi, Magfira SULFIANTI, SULFIANTI Suprianto Suprianto Suprianto Suprianto Suprianto Sutrisna Sutrisna Sutrisnawati Mardin Syaifuloh, Syaifullah Syech Zainal Tulak, David Pebriyanto Windarsih, Yulia Yusril Ihza Mahendra Yusril Ihza Mahendra Zulkaidah - -, Zulkaidah -