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All Journal Jurnal Akuakultur Rawa Indonesia Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Ilmu-Ilmu Perikanan dan Budidaya Perairan Jurnal Pengabdian Kepada Masyarakat Sakai Sambayan An-Nuha : Jurnal Kajian Islam, Pendidikan, Budaya Dan Sosial Jurnal Sumberdaya Akuatik Indopasifik MODELING: Jurnal Program Studi PGMI Jurnal Riset Akuakultur Jurdimas (Jurnal Pengabdian Kepada Masyarakat) Royal Jurnal Mangifera Edu Kumawula: Jurnal Pengabdian Kepada Masyarakat SCAFFOLDING: Jurnal Pendidikan Islam dan Multikulturalisme Scholastica : Jurnal Pendidikan dan Kebudayaan PELAGICUS: Jurnal IPTEK Terapan Perikanan dan Kelautan Reswara: Jurnal Pengabdian Kepada Masyarakat Muaddib: Islamic Education Journal Jurnal PKM Manajemen Bisnis JOMPA ABDI: Jurnal Pengabdian Masyarakat Ummul Qura : Jurnal Ilmiah Institut Pesantren Sunan Drajat (INSUD) Lamongan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Quagga: Jurnal Pendidikan dan Biologi Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Journal of Fisheries & Marine Jurnal Ilmiah Teknik Sipil (JIMATS) Lughawiyyat: Jurnal Pendidikan Bahasa dan Sastra Arab Journal of Islamic Economic Laws
Mochamad Syaifudin, Mochamad
Universitas Sriwijaya
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Journal : Journal of Fisheries

 1 
DNA Barcoding of Freshwater Eels Anguilla spp from Kuari River Based on Cytochrome C Oxidase Subunit I (COI) Gene Maulidya, Ainayyah; Syaifudin, Mochamad; Wijayanti, Marini
Jurnal Ilmiah Perikanan dan Kelautan Vol. 17 No. 1 (2025): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.vi.55006

Abstract

Graphical Abstract Highlight Research Freshwater eels in Kuari River consisted of two species marmorata and A. bengalensis Anguilla marmorata had the highest similarity of 99.82%-100%, while bengalensis indicated the highest identity of 99.84%-100% to the same species in the GenBank database. Phylogenetic species indicated that Anguilla marmorata and Anguilla bengalensis form two different sub-clusters. The water quality in the Bengkulu Kuari River were still in tolerance for fish growth and survival.     Abstract Freshwater eels (Anguilla spp.) are classified in the family Anguillidae, and included in the catadromous group. This study aimed to determine the COI gene sequences of the mitochondrial DNA, analyze the genetic distances, and phylogenetics, and characterize the physical and chemical parameters of between freshwater eels habitat in the Kuari River Bengkulu. This research was conducted from November 2020 – April 2021. The methods used in barcoding eel species were DNA isolation, DNA amplification using PCR (Polymerase Chain Reaction), electrophoresis, and sequencing of COI gene regions in mtDNA. The COI mtDNA gene fragments were obtained from PCR results with an annealing temperature of 50°C for 30 seconds in 35 cycles. BLASTn analysis of eel samples AM3 and AM4 had the highest similarity of 99.82%-100% to Anguilla marmorata, and samples AB2-AB5 indicated the highest identity of 99.84%-100% to Anguilla bengalensis. Phylogenetic species indicated that Anguilla marmorata and Anguilla bengalensis form two different sub-clusters. The water qualities in the Bengkulu Kuari River were temperatures 26.5-27.5oC, pH 7.1-8.7, dissolved oxygen 6.19-9.54 mg L-1, brightness 21-47 cm, ammonia 0.16-0.41 mg L-1, total alkalinity 20–52 mg L-1, TDS 33–49 mg L-1, salinity 0.3-0.4 ppt and water velocity 0.5-0.8 ms-1. The COI gene in DNA barcoding is very appropriate to be used for the identification of Anguilla spp species by comparing the DNA sequence of the COI gene with the existing database in the Genbank.
Co-Authors Ade Irawan Agus Oman Sudrajat Agus Putra AS Ahmad Fali Oklilas Ahmad Musadad Akhmad Taufiq, Mirwan Alimuddin Anita Desiani Annisa Siregar Anugerah Al-Amin Mangkunegara Apit Fathurohman Ari Prastiawan Aritonang, Lilis Pritamasari Bakhruddin, Mukhammad Batubara, Agung Setia Dade Jubaedah Dade Jubaedah Danang Yonarta Diah Kartika Sari Dini Puspa Monica Dwi Wulan Sari Elisa Wildayana Esti Susiloningsih Fachry Abda El Rahman Fatimatur Rusydiyah, Evy Fauzi, Alfa Zaidanil Fauziyah Fauziyah Ferdinand Hukama Taqwa Gion Tanbao Suselin Hamdani, Hijral Herpandi . Humairani, Rindhira Ilham Zulfahmi Indah Widiastuti Indah Widiastuti Indriani Agustini Jambak, Muhammad Ihsan Khazin, A. Mufti Khoirun Nasik Komar Sumantadinata Madyasta Anggana Rarassari Marini Wijayanti Marsi Marsi Marsi Marsi Maulidya, Ainayyah Mirna Fitrani Mirna Fitrani Mirna Fitrani Mohamad Amin muhammad Fery Artha Muhammad Sofwan bin Harizan Muhammad Syaikhon Mukri, Retno Cahya Muliari Muliari Muslim Muslim Muslim Muslim Muslim Nahidloh, Shofiyun Prily Lovian Putra Pujiati, Tri Purnama, Nanda Rizki Rarassari, Madyasta Anggana Ratu Siti Aliah Retno Cahya Mukri Retno Cahya Mukti Rinto . Rinto Rinto Rossanty, Elydia Sefti Heza Dwinanti Sefti Heza Dwinanti Sefti Heza Dwinanti, Sefti Heza Selly Oktarina Sumantriyadi, Sumantriyadi Susi Lestari SUSI LESTARI Syaikhu, Ahmad Syamsi, Ahmad Badarus Tanbiyakur Tanbiyaskur Tanbiyaskur Tanbiyaskur Tanbiyaskur Tanbiyaskur Tanbiyaskur, Tanbiyakur Tanbiyaskur, Tanbiyaskur Taqwa, Ferdinand Hukuma Tisnawati UTUT WIDYASTUTI Wahid Nur Tualeka, Muhamad Yanal Hadid Yonarta, Danang YULISMAN Yusrizal Akmal Zahro, Umi Indasyah