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All Journal Jurnal Kesehatan Andalas The Indonesian Biomedical Journal Sari Pediatri Molecular and Cellular Biomedical Sciences (MCBS) Buletin Ilmiah Nagari Membangun Journal of Midwifery Majalah Kedokteran Andalas Indonesian Journal of Global Health research Jurnal Kebidanan Indonesia Jurnal Ilmu Kesehatan Indonesia (JIKSI) International Journal of Medicine and Health Journal of Pharmaceutical and Sciences. Jurnal Otorinolaringologi Kepala dan Leher Indonesia (JOKLI) Malahayati International Journal of Nursing and Health Science
Hirowati Ali
Bagian Biokimia Fakultas Kedokteran Universitas Andalas
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Pengaruh Pemberian Mesenchymal Stem Cells Wharton's Jelly terhadap Ekspresi Gen Sirtuin 1 pada Tikus Model Alzheimer Havina Nurul Aizah; Amel Yanis; Hirowati Ali; Tofrizal Tofrizal; Cimi Ilmiawati; Dessy Arisanty
Jurnal Kesehatan Andalas Vol 12, No 1 (2023): Online March 2023
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v12i1.2140

Abstract

Mesenchymal Stem Cells Wharton's Jelly (MSC-WJ) has become a new hope for Alzheimer's disease due to its capability to suppress Amyloid-beta (Aβ) plaques formation. It is found that people with Alzheimer's disease have a lower expression of Sirtuin-1 because of the oxidative stress that was produced by Aβ plaques. Objective: To observe the Sirtuin-1 gene in Alzheimer's disease rat models that AlCl3 was inducing after being given MSC-WJ. Methods: This was an experimental study with a post-test-only control group design, including three groups with a total sample of 18 samples of Alzheimer RNA rat models. Group-1without AlCl3 and MSC-WJ; Group-2 with AlCl3; Group-3 AlCl3 and MSC-WJ. Mean value of the Sirtuin-1 gene was obtained by the Image-J application using a semiquantitative method which was then tested using the One Way ANOVA test. The study was significant if the p-value < 0,05. Results: This study found that the mean ± standard error value of the Sirtuin-1 gene in three groups were 0.18±0.04; 0.16±0.03; and 0.36±0.06 respectively. The statistical test showed a significant difference between the three groups (p = 0.014). Conclusion: There was an increased Sirtuin 1 gene expression in Alzheimer’s rat Model after being given Mesenchymal Stem Cells Wharton’s Jelly.Keywords:  Alzheimer, MSC-WJ, Sirtuin-1
Pengaruh Ekstrak Daun Kemangi (Ocimum Basilicum) terhadap Ekspresi TNF-Α pada Tikus Diabetes Melitus Gestasional Muhammad Faisal Rachman; Hirowati Ali; Siti Nurhajjah
Jurnal Ilmu Kesehatan Indonesia Vol 4 No 3 (2023): September 2023
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jikesi.v4i3.762

Abstract

Latar Belakang: Jumlah penderita diabetes melitus gestasional terus meningkat di seluruh dunia. Kondisi hiperglikemia yang terjadi pada masa kehamilan dapat memicu terjadinya stress oksidatif yang dapat menstimulasi peningkatan ekspresi Tumor Necrosis Factor-α (TNF-α). Ekstrak daun kemangi (Ocimum basilicum) mengandung zat antidiabetes dan antioksidan. Penelitian ini bertujuan untuk melihat efektifitas ekstrak daun kemangi (Ocimum basilicum) terhadap ekspresi TNF-α pada tikus model diabetes melitus gestasional. Objektif: Untuk mengetahui pengaruh pemberian ektrak daun kemangi (Ocimum basilicum) terhadap ekspresi TNF-α pada tikus model diabetes melitus gestasional. Metode: Penelitian ini merupakan penelitian eksperimental dengan menggunakan metode post test only control group design. Sampel penelitian menggunakan bahan biologis tersimpan dari RNA tikus yang telah diinduksi streptozotocin 40 mg/KgBB sebagai animal model DMG pada manusia yang disimpan di Lab Biomedik FK Unand. Hasil: Ekspresi TNF-α pada kontrol negatif, kontrol positif, perlakuan satu dan perlakuan dua berturut-turut adalah 1,01, 1,06, 0,95, dan 0,61. Kesimpulan: Menunjukkan adanya pengaruh pemberian ekstrak daun kemangi (Ocimum basilicum) terhadap ekspresi TNF-α pada tikus model diabetes melitus gestasional.
Pengaruh Pemberian Mesenchymal Stem Cells Wharton’s Jelly terhadap Ekspresi Gen PPAR-γ pada Tikus Alzheimer Nabila Priscilla Putri; Hirowati Ali; Tofrizal Tofrizal; Eryati Darwin; Restu Susanti; Hasmiwati Hasmiwati
Jurnal Kesehatan Andalas Vol 12, No 2 (2023): Online July 2023
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v12i2.2142

Abstract

The increased production of beta-amyloid marks Alzheimer's disease. PPAR-γ shows involvement in Alzheimer's disease. PPAR-γ Gene is suspected to reduce beta-amyloid plaques and reduce inflammation and oxidative stress in Alzheimer's disease. Mesenchymal Stem Cells Wharton's Jelly (MSC-WJ) is expected to increase the PPAR-γ Gene expression. Objective:  To observed the PPAR-γ Gene expression in Alzheimer's Mice. Methods: The mice are induced using AlCl3 and were given MSC-WJ. This study was an experimental study with a post-test-only control group design on 18 samples of RNA from Alzheimer's mice that were divided into 3 groups. Negative control group (K-), positive control group (K+), and experimented group (P). The mean of PPAR-γ gene expression was obtained by comparing the PPAR-γ gene with the GAPDH gene. This study used semiquantitative methods using ImageJ. Data analysis was used with the Kruskal-Wallis test. It is significant if the value is <0,05. Results: The mean ratio of PPAR-γ gene expression that was obtained in K-, K+, and P were 0.12, 0.06, and 0.08, respectively. There were significant differences between each group, with a p-value of 0,023 (p<0,05). Conclusion: Giving MSC-WJ could increase the PPAR-γ gene expression. Further study using real-time PCR is highly needed to increase the MSC-WJ treatment in patients with Alzheimer's disease.Keywords:  Alzheimer disease, MSC-WJ, PPAR- γ
Exploring the Effects of Bone Marrow Mesenchymal Stem Cells on Amyloid Plaque Reduction in a Rat Model of Alzheimer's Disease Annita Annita; Gusti Revilla; Hirowati Ali; Almurdi Almurdi
Majalah Kedokteran Andalas Vol 46, No 6 (2023): Online Oktober
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v46.i6.p1036-1044.2023

Abstract

Objective: This study aimed to evaluate the therapeutic potential of Bone Marrow-derived Mesenchymal Stem Cells (BM-MSCs) in mitigating amyloid plaque formation in Alzheimer's disease induced by Aluminum Chloride (AlCl3) in rats. Methods: Rats were divided into three groups: negative control, positive control (AlCl3-induced), and treatment (AlCl3 + BM-MSCs). Cognitive function was assessed five days post-induction, and amyloid plaques were quantified using Congo Red staining in the cortex and hippocampus. Results: A significant reduction in amyloid plaque was observed in rats treated with BM-MSCs. The proportion of stained areas decreased from 1.88 to 1.73 in the cortex and from 1.61 to 1.47 in the hippocampus, compared to AlCl3-only controls. Conclusion: BM-MSCs demonstrated a moderate but statistically significant reduction in amyloid plaque in a rat model of Alzheimer's disease, indicating their therapeutic potential.
EDUKASI KESEHATAN DAN PEMERIKSAAN GOLONGAN DARAH PADA MURID SD NEGERI 13 DI DESA TUNGKAL SELATAN KECAMATAN PARIAMAN UTARA Yustini Alioes; Husnil Kadri; Eti Yerizel; Hirowati Ali; Rauza Sukma Rita; Desy Arisanti
BULETIN ILMIAH NAGARI MEMBANGUN Vol. 4 No. 2 (2021)
Publisher : LPPM (Institute for Research and Community Services) Universitas Andalas Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/bina.v4i2.289

Abstract

Elementary school students are consumers who are among those who consume the most food and beverages. Food and drinks sold around the school contain many hazardous substances such as dyes that have been banned from circulating (Rhodamine B in iced drinks, jelly drinks, and so on) and other additives such as formalin which is used as a chewy substance in meatballs sold in carts and restaurants that can cause acute poisonings such as allergies, coughs and chronic poisonings such as cancer of the liver, kidneys and other organs. The purpose of this activity is to increase knowledge about substances in food and an introduction to blood group examination. This activity was carried out at SD Negeri 13 Tungkal Selatan Village in North Pariaman District. The method of activity is in the form of counseling and demonstration. According to the survey before the blood group examination, it turned out that most of the elementary school students did not know their blood type. Before the blood group examination, the students were also given knowledge about determining a person's blood type. After this counseling was carried out, respondents became more aware of the impact of food/snacks on health, and respondents knew their respective blood types. Of the 64 students, there are 22 A, 17 B, 12 AB, 13 O. To avoid problems related to food and blood type. There is a need for continuous health education for elementary school students.
Efek Terapi Adjuvan Gel Kolagen terhadap Kadar Matriks Metaloproteinase-8 Saliva pada Gingivitis Pasca Scaling Root Planing Haria Fitri; Nila Kasuma; Hardisman Hardisman; Hirowati Ali
Journal of Pharmaceutical and Sciences JPS Volume 9 Nomor 2 (2026)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v9i2.1500

Abstract

Gingivitis is a reversible inflammatory condition of the gingival tissue without attachment loss, primarily caused by plaque accumulation, which can trigger an immune response. Matrix metalloproteinase-8 (MMP-8) is an enzyme that contributes to collagen degradation. Scaling and root planing (SRP) is the standard therapy; however, it may induce a transient inflammatory response and result in suboptimal tissue regeneration, thereby necessitating adjunctive therapy. This study aimed to evaluate the effect of adjunctive collagen gel therapy on salivary matrix metalloproteinase-8 levels in gingivitis. This experimental study employed a pre- and post-test control group design involving 50 subjects divided into five groups: healthy control, gingivitis treated with SRP, and gingivitis treated with SRP combined with 1%, 2%, and 4% collagen gel. Salivary MMP-8 levels were measured to assess the inflammatory response. Statistical analysis revealed that collagen gel at concentrations of 1% and 2% had a significant effect on MMP-8 levels. In conclusion, Collagen gel at 1% and 2% concentrations significantly modulated salivary MMP-8 levels, suggesting a potential role in the tissue remodeling phase following SRP.
The Effect of Kemangi Giving on HIF-1α Expression on DMG Model Rats Muhammad Iqbal Dasril; Hudila Rifa Karmia; Hirowati Ali
International Journal of Medicine and Health Vol. 3 No. 3 (2024): September : International Journal of Medicine and Health (IJMH)
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/ijmh.v3i3.3854

Abstract

The incidence of diabetes continues to increase worldwide. The state of hyperglycemia during pregnancy can trigger oxidative stress which can result in hypoxic conditions in the tissues characterized by an increase in HIF-1α. Basil which contains antioxidants is expected to reduce the expression of HIF-1α. This study aims to determine the effect of the administration of basil on the expression of HIF-1α in gestational diabetes mellitus model rats. To determine the effect of the administration of basil extract (Ocimum basilicum) on the expression of HIF-1α in gestational diabetes mellitus model rats. This research is experimental research conducted using the post test only control group design method. The research sample used biological material stored from rat RNA that had been induced by streptozotocin 40 mg/kgBB as an animal model of DMG in humans stored in the Biomedical Lab, FK Unand. The results of this study indicate the effect of the administration of basil extract (Ocimum basilicum) on the expression of HIF-1α in gestational diabetes mellitus model rats. Researchers used conventional PCR tools and still manually used ImageJ to look for HIF-1α expression values from electrophoresis results, so the possibility of bias still exists.
Relationship Between Dried Amnion Sheet and Dry Powdered Amnion with Changes in Wound Area in Acute Wounds Ferdinand Ferdinand; Afriwardi Afriwardi; David Sontani Perdanakusuma; Hirowati Ali
Indonesian Journal of Global Health Research Vol 7 No 5 (2025): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v7i5.6906

Abstract

Dried sheet amnion has long been used as a wound treatment that provides better results than standard general care. In addition to dried amnion sheet, dry powdered amnion preparations have begun to be used for wound care. This study aims to evaluate the effectiveness of dry powdered amnion compared to dried amnion sheets in wound healing, and to compare both treatments with standard acute wound care. Sixty-three healthy Wistar rats were randomly divided into three groups: dried amnion sheet treatment group, dry powdered amnion treatment group, and standard wet wound care control group. The research procedure began with the administration of anesthesia using penthotal at a dose of 10mg/kgBW intramuscularly. The backs of the rats were shaved and a 2x1cm wound was designed using a GOAL razor blade after disinfection with 10% betadine solution and 1:30 savlon. Wounds were made on the backs of the rats, and treatments were given according to the group. The wound area was evaluated at time points 6 hours after treatment, day 2 and day 5. Statistical analysis was performed to compare the effectiveness of the treatments. Both treatments with dry powdered amnion and dried amnion sheets significantly reduced the wound area compared to the control group. However, treatment with dried amnion sheets showed higher efficacy in reducing wound area compared to treatment with dry powdered amnion. Statistical analysis confirmed significant differences in wound area reduction between groups. Dry powdered amnion and dried amnion sheets were effective in reducing wound area compared to standard wet wound care. Treatment with dried amnion sheets showed greater efficacy than treatment with dry powdered amnion.
Perbedaan Ekspresi Transforming Growth Factor Beta 1 antara Rinosinusitis Kronis dengan Polip dan Tanpa Polip Rahmadona Rahmadona; Bestari Jaka Budiman; Effy Huriyati; Hirowati Ali; Hafni Bachtiar
Jurnal Otorinolaringologi Kepala dan Leher Indonesia Vol. 1 No. 1 (2022)
Publisher : Fakultas Kedokteran Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jokli.v1i1.23

Abstract

Latar belakang: Rinosinusitis kronis (RSK) merupakan inflamasi pada mukosa hidung dan sinus paranasal. Rinosinusitis kronis berdasarkan perbedaan ekspresi sitokin dan pola remodeling inflamasi dibedakan menjadi dua fenotipe, RSK dengan polip dan RSK tanpa polip. Transforming growth factor–?1 (TGF–?1) merupakan salah satu sitokin yang berperan pada remodeling jaringan.       Tujuan: Mengetahui ekspresi gen TGF–?1 pada RSK dengan polip dan RSK tanpa polip. Metode: Penelitian analitik komparatif menggunakan desain potong lintang (cross sectional comparative study) pada 12 responden RSK dengan polip dan 12 responden RSK tanpa polip. Sampel diambil saat operasi Bedah Sinus Endoskopi Fungsional (BSEF) pada jaringan polip dan mukosa sinus etmoid atau maksila. Semua sampel dilakukan pemeriksaan ekspresi gen dengan metode Real Time Polymerase Chain Reaction (RT-PCR). Data dianalisis dengan SPSS, dikatakan bermakna jika p<0,05. Hasil: Ekspresi TGF-?1 pada RSK tanpa polip lebih tinggi (18,63±24,58) dibandingkan RSK dengan polip (2,82±4,02). Secara statistik perbedaan antara RSK polip dan RSK tanpa polip ini bermakna (p<0,05). Kesimpulan: Terdapat peningkatan ekspresi TGF-?1 pada RSK tanpa polip yang bermakna secara statistik dibandingkan dengan RSK dengan polip.  
Peran Ki67 pada Karsinoma Nasofaring Tika Hakikah; Sukri Rahman; Hirowati Ali
Jurnal Otorinolaringologi Kepala dan Leher Indonesia Vol. 3 No. 1 (2024)
Publisher : Fakultas Kedokteran Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jokli.v3i1.64

Abstract

Latar Belakang: Pendahuluan: Karsinoma nasofaring memiliki rekurensi atau metastasis jauh, yang menandakan suatu adanya aktifitas sel yaitu proliferasi sel. Penilaian adanya suatu proliferasi sel memerlukan biomarker, salah satunya Ki67 yang sampai saat ini sudah dikenal baik sebagai penanda proliferasi sel. Tujuan: Mengetahui peran Ki67 pada karsinoma nasofaring. Tinjauan Pustaka: Karsinoma nasofaring adalah tumor ganas sel skuamosa yang terjadi di daerah perbatasan epitel nasofaring dimana epitel kubus beralih menjadi epitel skuamosa. Pada stadium dini tidak mudah dideteksi karena secara anatomis terletak jauh di dalam kepala. Ki67 berhubungan erat dengan mitosis dan aktivitas proliferasi tumor ganas serta kinerja pengobatan kanker nasofaring. Kesimpulan: Ekspresi Ki67 yang tinggi merupakan penanda untuk prognosis buruk pada karsinoma nasofaring. ABSTRACT Background: Introduction: Nasopharyngeal carcinoma has recurrence or distant metastasis, which indicates a cell activity, namely cell proliferation. Assessment of the presence of a cell proliferation requires biomarkers, one of which is Ki67 which has been established as a marker of cell proliferation. Objective: To determine the role of Ki67 in nasopharyngeal carcinoma. Literature Review: Nasopharyngeal carcinoma is a malignant squamous cell tumor that occurs in the border area of the nasopharyngeal epithelium where the cuboidal epithelium turns into the squamous epithelium. At an early stage, it is difficult to detect because it is anatomically located deep in the head. Ki67 is closely related to the mitotic and proliferative activity of malignant tumors and the treatment performance of nasopharyngeal carcinoma. Conclusion: High Ki67 expression is a marker for poor prognosis in nasopharyngeal carcinoma.    
Co-Authors Afrah Diba Faisal Afriwardi Afriwardi Almurdi Almurdi Amel Yanis Annita ., Arifin, Yulia Aswiyanti Asri Bestari Jaka Budiman David Sontani Perdanakusuma Desmawati Desmawati Desmiwarti Desmiwarti Dessy Arisanty Desy Arisanti Desy Arisanti Dhina Lidya Lestari Dinda Aprilia Dio Kurniawan Djong Hon Tjong Effy Huriyati Eryati Darwin Eti Yerizel Fadina Rizki Febianne Eldrian Febry Mutiariami Dahlan Ferdinand Ferdinand Fitri Amita Fitri Ramadhaniati Gusti Revilla Hafifa Ramadhani Hafni Bachtiar Hafni Bachtiar Hardisman Haria Fitri Hasmiwati Havina Nurul Aizah Hudila Rifa Karmia Husnil Kadri Ilmiawati, Ilmiawati Indah Muthara Intan Putri Syamtoni Johanes C Mose Johanes C. Mose Joserizal Serudji Masrul Djalal Masrul Masrul Muhammad Arif Muhammad Faisal Rachman Muhammad Iqbal Dasril Nabila Priscilla Putri Nila Kasuma Nora Harminarti Nur Indrawati Lipoeto Nuzulia Irawati Rahmadona Rahmadona Ranti Verdiana Rauza Sukma Rita Restu Susanti Rizki Rahmadian Rozetti Rozetti Sisca Dwi Yarni Siti Aisyah Siti Nurhajjah Sukri Rahman Tika Hakikah Tio Firmansyah Tofrizal Tofrizal Vivi Sofia Wahyu Agnata Surya Widia Febrina Wildayani, Desi Yuastika Puspita Sari Yusrawati Yusrawati Yusri Dianne Jurnalis Yusticia Katar Yustini Alioes Zilfi Yola Pitri