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Journal : INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY

Blumea balsamifera and Sargassum aquifolium extracts reduce fatty liver damage through lipid metabolism signalling pathways Widhiantara, I Gede; Wiradana, Putu Angga; Permatasari, Anak Agung Ayu Putri; Sari, Ni Kadek Yunita; Rosiana, I Wayan; Sandhika, I Made Gde Sudyadnyana; Panjaitan, Novaria Sari Dewi
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 6 No 2 (2024): Promising and Valuable Research Towards Diagnosis, Prognosis and Treatment of Dis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v6i2.5697

Abstract

Non-alcoholic fatty liver disease (NAFLD) is a condition marked by excessive fat accumulation in the liver and poses a significant health challenge. The leaves of Blumea balsamifera and Sargassum aquifolium have been reported to have anti-atherogenic effects. This study aims to determine the effectiveness of B. balsamifera extract (BBLE) and S. aquifolium extract (SAE) in preventing and treating liver fat accumulation in Wistar rats induced by a high-cholesterol diet through the expression of the AMP-activated protein kinase (AMPK)/ Sirtuin 1 (SIRT1)/peroxisome proliferator-activated receptor γ (PPARγ) pathway, and the leptin receptor. The experimental design of this study is laboratory-based, involving, 20 Wistar rats were fed a high-cholesterol diet over a period of 21 days. The rats were divided into four groups for the evaluation of BBLE and SAE effect: negative control (P0): induced with a high-cholesterol diet + distilled water, positive control (P1): induced with a high-cholesterol diet + simvastatin, P2: induced with a high-cholesterol diet + 4 mg/kg/bw BBLE, and P3: induced with a high-cholesterol diet + 4 mg/kg/bw BBLE and 4 mg/kg/bw SAE. The treatment duration extended over three months. Immunohistochemical analyses were performed on liver tissues to measure AMPK, SIRT1, PPARγ, and leptin receptor expression. The results indicated that leptin expression was lower in the BBLE+SAE group compared to the simvastatin group, and differences were significant between the BBLE and BBLE+SAE groups. No significant differences were noted in AMPK, SIRT1, and PPARγ expression between the simvastatin and BBLE+SAE groups (p≥0.05). In conclusion, BBLE and SAE effectively reduce liver lipid accumulation and enhance fat metabolism in hypercholesterolemic rats.
Antidyslipidaemic effect of Ipomoea batatas L. extract with increased PCSK-9 levels in atherosclerotic rats Yasa, I Wayan Putu Sutirta; Jawi, I Made; Mahendra, Agung Nova; Wiradana, Putu Angga; I Gede Widhiantara
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 8 No 1 (2026): Integration of Molecular Approaches in Addressing Drug Resistance and Changing Gl
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v8i1.7260

Abstract

Atherosclerosis is the largest cause of mortality worldwide, and Low-Density Lipoprotein (LDL) is a primary risk factor for this illness. The purpose of this research was to determine the effect of purple sweet potato (Ipomoea batatas L.) extract on cholesterol, LDL, PCSK9, and MDA levels in rats fed a high-cholesterol diet. This true experimental study used a randomised pre-test-post-test control group design. Wistar rats were randomly assigned to three groups. Purple sweet potato extract (PSPE) was prepared by 24-hour maceration with 70% ethanol. All rats were fed a high-cholesterol diet for 14 days, followed by pre-test measurements of cholesterol, LDL, PCSK9, and MDA on day 15. Rats were then randomly divided into control (no treatment), PSPE (200 mg/day), and Simvastatin (0.9 mg/kg BW) groups. After one month of treatment, post-test analyses were performed for all parameters. The results showed an increase in serum cholesterol, LDL and MDA levels in rats fed a high-cholesterol diet (p<0.05) compared to the PSPE and Simvastatin groups. The high-cholesterol diet also resulted in a significant (p<0.05) decrease in PCSK9 when compared to the PSPE and Simvastatin groups. In the PSPE group there was a decrease in cholesterol, LDL, and MDA, and PSPE (p<0.05) compared to the control group. In the Simvastatin group, the increase in PCSK9 was very low compared to the PSPE group. PSPE has antidyslipidaemic activity by reducing total cholesterol status, LDL, and oxidative stress while promoting PCSK-9 function. Further research is still needed to provide a comprehensive understanding of the mechanism of action of PSPE as an antioxidant source in patients with atherosclerosis.
Co-Authors Abd. Rahman As-syakur Adam Saba Anggara Adi Ariyanto Wibisono Adi Sofyan Ansori, Muhammad Adnorita Fandah Oktariani Afta Daniel Zato Waruwu Agoes Soegianto Agung Nova Mahendra Agung Wahyu Permadi Agustono Agustono Anak Agung Ayu Putri Permatasari Anak Agung Ngurah Subawa Aondohemba Samuel Nege Aprodita, Ni Wayan Seila Aris Jatmiko Aryaswari, Ni Kadek Dwi Liopita Aulia Iefan Datya Bagus Komang Satriyasa Baladrat, Nur Komariah Bambang Pontjo Priosoeryanto Bambang Yulianto Bambang Yulianto Corne, Yohanes Deny Suhermawan Yusup Dewa Ayu Angga Pebriani Dewa Ayu Intan Tirta Sari Dewi, Pande Putu Indira Prima Eka Putri Suryantari Elizabeth, Gladys Eva Harlina Fahror Rosi Fimaputra, Jatu Maranatha Fransiskus Jimmy Roga Garry Ganvis Jonteo Hani Gede Surya Indrawan Hanipa, Petronela Herdiansyah, Mochammad Aqilah I Gede Widhiantara I Ketut Wija Negara I Made Gde Sudyadnyana Sandhika I Made Jawi I Made Murna I Made Saka Wijaya I Made Sara Wijana I Made Sara Wijana I Made Wisnu Adhi Putra I Wayan Putu Sutirta Yasa I Wayan Rosiana Ida Ayu Mirah Meliana Dewi Juniartini, Nyoman Sweet Kismono Kudoasmoro Komang Kartika Indi Swari Kusala, Muhammad Khaliim Jati Kusala Kusuma, Nathania Eka Putri Lanniari, Nafrina Luh Putu Widiastini M. Rheza Rizki Syahputra Made Nyandra MADE PHARMAWATI Matilda, Yuliana Matius Victorino Ola Dame Mirni Lamid Moch. Nurhudah Mochammad Aqilah Herdiansyah Muhammad Taufiq Shidqi Naw, Sin War Ni Kadek Dwipayani Lestari NI KADEK YUNITA SARI Ni Komang Widiastuti Ni Luh Sunarsih Ni Luh Watiniasih Ni Made Wagi Ambakesari Ni Putu Adriani Astiti Ni Putu Putri Wijayanti Ni Wayan Ayu Wiartini Ni Wayan Deswiniyanti Novaria Sari Dewi Panjaitan Nurhudah, Moch. Nurina, Dien Uma Nwet Darli Kyaw Zaw Okabayashi, Tamaki Otie Dylan Soebhakti Hasan Panjaitan, Novaria Sari Dewi Panjaitan, Novaria Sari Dewi Panjaitan Pelupessy, Yesha Ainesis El Gracianita Permatasari , Anak Agung Ayu Putri Permatasari, Anak Agung Ayu Putri Permatasari, Anak Agung Ayu Putri Permatasari Prastyadi Wibawa Rahayu Putri, Fadillaisyia Riandani Putu Eka Sudaryatma Putu Eka Sudaryatma Rachmi Ridho Raden Joko Kuncoroningrat Susilo Rahardja, Viryanando Evan Rahardjo, Sinung Rahayu, Tb. Haeru Rahmadi Prasetijo Razaq, Imanuddin Retno Wulansari Rizhar Eman Karunia Akbar Rizqy, Aimatun Nisfia Rostantinata, Riski Sari, Ni Kadek Yunita Sari Sektiana, Sinar Pagi Setyo Budi Kurniawan Sin War Naw Sinung Rahardjo Siregar, Lindah Erlia Safira Sri Puji Astuti Wahyuningsih Sugama, I Ketut Surachmi Setiyaningsih Sutrisno, Sutrisno Teguh Hari Sucipto, Teguh Hari Triyanto , Triyanto Utari, Heny Budi Viryanando Evan Rahardja Waruwu, Ernawati Wiartini, Ni Wayan Ayu Wiartini Wibisono, Adi Ariyanto Widiiastuti, Ni Komang Wijaya, I Made Saka Win Darmanto Yesha Ainensis El G. Pelupessy Yohanes Corne