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All Journal HAYATI Journal of Biosciences ACTA VETERINARIA INDONESIANA Hemera Zoa Media Veteriner Jurnal Sain Veteriner Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter BIOTROPIA - The Southeast Asian Journal of Tropical Biology ZOO INDONESIA ARSHI Veterinary Letters Dinamika Informatika: Jurnal Ilmiah Teknologi Informasi Indonesian Journal of Applied Research (IJAR) PARADIGMA Borneo Journal of Science and Mathematics Education Borneo Journal of Primary Educaton Al-Muraqabah: Journal of Management and Sharia Business Jurnal Kedokteran Hewan Jurnal Kajian Ekonomi dan Manajemen Indonesia Policy Brief Pertanian, Kelautan, dan Biosains Tropika Al-Insyiroh: Jurnal Studi Keislaman Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Tarbiyah Jamiat Kheir Research Economics Management and Business Jurnal Bioteknologi & Biosains Indonesia

MUHAMMAD AGIL

Bagian Reproduksi Dan Kebidanan Departemen Klinik Reproduksi Dan Patologi Fakultas Kedokteran Hewan Institut Pertanian Bogor, Bogor

Author-ID : 3194459

Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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FA-9 Reproductive Efficiency of Brahman Cross Cattle Using Artificial Insemination with Frozen Semen from Bali, Brahman, Limousin, and Simmental Cattle Husnul Khotimah; Muhammad Agil; Bakti Tamba; I Ketut Karya Wisana; Sutrisnak Sutrisnak; Harianto Budi Rahardjo; Tuty L Yusuf
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (647.995 KB)

Demand and consumpsion of beef meat increases every year in Indonesia. The increase of demand is not followed by the availability supply of beef cattle production nationaly. Statistical data from Direktorat Jenderal Peternakan Indonesia showed that on the year of 2016 the demand of beef was 604.968 ton and the production of beef was only 354.770 ton. The goverment imported of beef and cattle to fulfill the lack of supply, as of 2016 there was 116.761 ton beef and 1.298.560 cattle imported to Indonesia(1).Indonesia has determined to achieve beef self–sufficiency by 2024. To achieve that goal the goverment want to increase the cattle population nationaly by requiring the cattle and buffalo farm industry to oblige the breeding programe. The goverment made some policy including Upsus SIWAB and an obligation of the feedloters of imported cattle to do breeding. According to PERMENTAN no 49 thn 2016 and it’s add on PERMENTAN no 2 thn 2017, importation of beef cattle must meet the ratio 1:5 between breeding cattle and feeder cattle.Reproductive efficiency is a parameter used to determine the success of breeding programe. Reproductive eficiency is a measure of the ability if a cow to become pregnant and produce offspring(2). Optimalization of reproductive efficiency can be one of means to increase national cattle population.Brahman Cross is one of the most common imported cattle breed to Indonesia. Husbandry and reproductive management play an important role to achieve the best result on cattle breeding programe. Feedloters who conduct breeding programe of Brahman Cross cattle (Breedlot) have the capacity and potential to increase the reproductive efficiency of their breeding programe due to they have capital and good management. Thus it is important to assess and measure the reproductive efficiency of Brahman Cross cattle artificially inseminated with frozen semen to increase the reproductive efficiency of beef cattle in the Breedlot.

AH-25 Sperm Morphology of the Javan Muntjak, Muntiacus muntjak muntjak Sri Wahyuni; Gholib Gholib; Wahono Esthi Prasetyaningtyas; I Ketut Mudite Adnyane; Srihadi Agungpriyono; Hamny Hamny; Muhammad Jalaluddin; Mustafa Sabri; Muslim Akmal; Muhammad Agil; Tuty Laswardi Yusuf
Media Veteriner Proceedings of The 5th Congress of Asian Association of Veterinary Anatomists (Asian AVA) 2015
Publisher : Media Veteriner

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Sperm Morphology of the Javan Muntjak, Muntiacus muntjak muntjak

FAECAL GLUCOCORTICOID MEASUREMENT AS INDICATOR STRESS IN WILD CRESTED MACAQUES (MACACA NIGRA): THE IMPORTANCE OF VALIDATION AND SAMPLE PROCESSING TECHNIQUES . Gholib; Taufiq P. Nugraha; Muhammad Agil; Iman Supriatna; Bambang Purwantara; Antje Engelhardt
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 4, No 2 (2014): Life Sciences
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (244.231 KB)

Monitoring of physiological stress in wild and/or endangered animals living in their natural habitat can be generated via measuring faecal glucocorticoid metabolites (FGCMs) through enzyme immunoassay technique. However, a careful validation of each enzyme immunoassays (EIAs) and methodological issues such as samples preservation, and extraction procedures must be a significant concern and validated because it may influence FGCMs levels. In this present study, the aim was to carry out a biological validation to find reliable EIAs that could be used for non-invasive measurement of glucocorticoid levels in crested macaques. Moreover, we compared three different techniques of preservation: lyophilisation, oven drying and field extraction and three different solvents for extraction: 80% methanol, 90% pure ethanol and 90% commercial alcohol (isopropyl alcohol) to find the simple and practical techniques for sample processing. For hormone analysis, we colIected 189 faecal samples from wild crested macaques living in the Tangkoko-Batuangus Nature Reserve, North Sulawesi, Indonesia. The results show that biological validation can be used to validate measurement of faecal glucocorticoid which 3α,11β-dihydroxy-etiocholanolone was the most reliable EIAsfor this species. Different preservation and solvent extraction were not significantly influenced levels of FGCMs (p0.05). In conclusion, Our study highlights the importance of biological validation of FGCMassays and presents practical techniques for the non-invasive monitoring of physiological stress in crested macaques.

Spermatogenesis and semen quality of male muntjak (Muntiacus muntjak muntjak) during antler growth periods Sri Wahyuni; Srihadi Agungpriyono; Muhammad Agil; Idawati Nasution; . Hamny; Tuty L. Yusuf
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (512.016 KB)

Muntjak (Muntiacus muntjak muntjak) belongs to Cervidae family which distributed in Java Island and Southern part of Sumatera. This cervid has been protected by Indonesian Government since 1999. In order to support breeding program of the species and to avoid them from extinction, itsreproductive biology such as spermatogenesis and the correlation to semen quality is important to be investigated. Therefore, the objective of this study was to examine spermatogenesis and semen quality of two adult male muntjaks during antler growth periods that consist of hard antler (H), casting (C), andvelvet antler (V). Testicular tissues and semen (ejaculates) were obtained by core needle biopsy and electroejaculation methods respectively. Testicular tissues were processed histologically and stained with periodic acid Schiff (PAS) to observe spermatogenesis whereas semen was evaluated to obtaine itsquality. The results showed that spermatogenic activities were detected in all antler periods which marked by PAS positive staining (magenta colour) of round and elongated spermatid acrosomes. In H period, spermatogenic activity was higher than those C and V periods. According to semen evaluation,motile spermatozoa were found with different concentration in all antler periods. The highest sperm concentration (x 106 spermatozoa/ml) in both of muntjaks was found in H period (506.25 ± 61.87), and slightly decreased in C (288.75 ± 37.12), and V periods (362.60 ± 17.68). These finding showed thatspermatogenesis to produce spermatozoa is taken place while muntjaks are in C and V periods with differ activities that provable with the existence of motile spermatozoa from ejaculates in both of male muntjaks. Therefore, muntjaks could provide reproductive function throughout the year of reproductiveaseasonality which is similar to the reeves and formosan muntjaks

Identifikasi Mikroba dari Lendir Estrus dan Deteksi Kebuntingan Sapi Kawin Berulang di Sleman, Yogyakarta Surya Agus Prihatno; Abdul Samik; Dea Indriani Astuti; Muhammad Agil; Usamah Afiff; Anriansyah Renggaman; Dedi Rahmat Setiadi; Yosua Kristian Adi
Jurnal Sain Veteriner Vol 39, No 3 (2021): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.70916

Repeat breeding is condition of cows that have normal or near normal estrous cycles but fail to become pregnant after several insemination. In this study, we investigated the microbes in the cervical and vaginal mucus of beef cattle and dairy cows that experience repeat breeding and detected the pregnancy after the third or more artificial insemination. A total of 14 beef cattle and 6 dairy cows that experience repeat breeding in the cattle herd in the Yogyakarta region of Indonesia were used as samples in this study. Cervical and vaginal mucus samples were collected using plastic sheet when the cow was re-estrus. The samples were put into the 5 ml Brain Heart Infusion Broth for bacterial isolation and identification. Pregnancy examination was carried out on day 45 after artificial insemination using ultrasound method. Bacteria that could be isolated and identified from cervical mucus and vaginal mucus of beef cattle and dairy cows included Bacillus sp., Staphylococcus epidermidis, Staphylococcus aureus, and Pseudomonas sp. Some cows detected positive in pregnancy examination even though the bacteria were presence in the cervical and/or vaginal mucus. There was various composition of bacteria found in the cervical mucus and vaginal mucus, of beef cattle and dairy cows with repeat breeding in livestock groups in Yogyakarta. The presence of bacterial in the cervical and vaginal mucus during estrus was not always become the causes of failed pregnancy.

Gambaran Umum Kajian Profil Hormon Steroid Menggunakan Metode Non-Invasif dari Sampel Feses R. Taufiq Purna Nugraha; Bambang Purwantara; Iman Supriatna; Muhammad Agil; Gono Semiadi
ZOO INDONESIA Vol 25, No 1 (2016): Juli 2016
Publisher : Masyarakat Zoologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52508/zi.v25i1.3029

Kajian terhadap profil hormon-hormon steroid merupakan kunci penting dalam upaya memahami aspek fisiologis satwa. Beberapa dekade terakhir telah dikembangkan metode alternatif untuk mengetahui profil hormon steroid, yaitu melalui pengukuran metabolit hormon steroid yang diekskresikan melalui ekskreta tubuh seperti feses. Metode tersebut dikenal sebagai metode non-invasif. Metode ini memungkinkan pengumpulan sampel secara terus menerus dalam jangka panjang dengan meminimalisasi gangguan terutama pada satwa liar. Kajian terhadap profil metabolit hormon steroid yang terukur dapat diaplikasikan antara lain untuk mengetahui status reproduksi, penentuan jenis kelamin, studi perilaku hingga monitoring tingkat stres satwa. Berbagai kajian dengan memanfaatkan metabolit hormon steroid telah berhasil diaplikasikan pada berbagai taksa vertebrata. Tulisan ini memberikan gambaran terkini mengenai aplikasi metode non-invasif untuk kajian profil metabolit hormon steroid dari sampel feses.

Analisis Kolorimetri Kadar Hemoglobin Darah dengan Metode Pencitraan Digital Menggunakan Desktop Scanner Koekoeh Santoso; Muhammad Agil; Ridzky Pratama
ARSHI Veterinary Letters Vol. 1 No. 2 (2017): ARSHI Veterinary Letters - November 2017
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Spektrofotometer adalah sistem baku berbasis kolorimetri dalam pengukuran kadar Hemoglobin (Hb). Penggunaan scanner untuk mengukur kadar Hb berbasis kolorimetri telah dilakukan sebagai sistem alternatif. Kurva baku kanal warna scanner yang paling curam adalah blue. Perbandingan kurva baku spektrofotometer dengan kanal warna blue menunjukan nilai y = 0.3131x + 0.0565 dengan R2 = 0.9954 dan angka konversi nilai absorbansi desktop scanner menjadi kadar Hb adalah 73.6. Hasil uji Pasangan T menghasilkan nilai p sebesar 0.325 atau tidak berbeda nyata pada p ˃ 0.05.

Potensi Alfa Enolase (ENO1) Membran Plasma Spermatozoa Sapi Bali Sebagai protein Antigenik Teguh Sumarsono; Bambang Purwantara; Iman Supriatna; Mohamad Agus Setiadi; Muhammad Agil
Acta VETERINARIA Indonesiana Vol. 10 No. 3 (2022): November 2022
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.10.3.262-269

Antibodi imunoglobulin G (IgG) merupakan salah satu immunoglobulin yang dikandung oleh antibodi anti-sperma (ASA) yang terdapat pada saluran reproduksi betina. Imunoglobulin G dapt berikatan dengan protein-protein yang berpotensi sebagai protein antigenik seperti alfa enolase (ENO1). Penelitian ini bertujuan untuk mengidentifikasi dinamika dan potensi ENO1 membran plasma spermatozoa sebagai protein antigenik serta kualitas spermatozoa sapi Bali dengan perlakuan 0,5 mg/ml IgG. Sampel penelitian adalah 16 ejakulat yang diperoleh dari 4 ekor pejantan. Motilitas spermatozoa dievaluasi menggunakan CASA, viabilitas melalui metode pewarnaan diferensial, Keutuhan Membran Plasma (MPU) menggunakan metode Hypo-osmotic Swelling Test (HOS-Test), nilai Mix Anti-globulin Reaction (MAR) diperoleh dari MAR-Test, sedangkan kuantitas ENO1 diukur dengan ELISA. Analisis data menggunakan analisis ragam RAK. Hasil penelitian menunjukkan bahwa perlakuan dengan IgG sampai 0,5 mg/ml menurunkan motilitas, kinematika spermatozoa, viabilitas, MPU dan kuantitas ENO1 membran plasma spermatozoa (P<0,01), serta meningkatkan nilai MAR-test (P<0,05). Penelitian menyimpulkan bahwa IgG dapat menurunkan kualitas spermatozoa dan kuantitas ENO1, sekaligus menunjukkan bahwa ENO1 membran plasma spermatozoa sapi Bali berpotensi sebagai protein antigenik.

THE EFFECTIVENESS OF PROGESTERONE HORMONE OR PGF2α AND hCG APPLICATION TO INCREASE REPRODUCTIVE EFFICIENCY OF POSTPARTUM OF ANESTRUS PE GOATS Muhammad Syawal; Tuty L Yusuf; Muhammad Agil
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): December
Publisher : Universitas Syiah Kuala

This study aimed to improve reproductive condition of postpartum anestrus of Etawa crossbreed (PE) goats using PGF2α hormone or progesterone (controlled internal drug release/CIDR-G) and improve pregnancy success from artificial insemination (AI) with human chorionic gonadotrophin (hCG) hormone administration. Administration of progesterone increased estrus by 100%, which was higher than the 70% produced from prostaglandin F2 alfa (PGF2α) administration. Estrus onset in CIDR group (35.80±13.33 hours) was faster than PGF2α group (45.6±9.2 hours). Clinical signs of vulva (redness and swollen) were more intense in CIDR-G group (80% and 70%, respectively) compared to PGF2α group (70% and 57%, respectively). Estrus duration in PGF2α group (52.17±3.34 hours) was longer than CIDR-G group (49.61±3.56 hours). Pregnancy success in group given hCG was 66.7% while in non-hCG group was 50%. In conclusion, CIDR-G and PGF2α administration is effective to induce estrus of postpartum anestrus PE goats. Administration of hCG at the onset of estrus is effective to improve pregnancy success.

ANATOMI DAN GAMBARAN ULTRASOUND ORGAN REPRODUKSI SELAMA SIKLUS ESTRUS PADA KUDA GAYO BETINA (Anatomy and Ultrasound Imaging of Reproductive Organs of Gayo Mares During Estrous Cycle) Juli Melia; Muhammad Agil; Iman Supriatna; Amrozi Amrozi
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

The present study examines anatomy of Gayo mare reproductive organs. This study used three sample of Gayo mare reproductive organs (n= 3) for observation of morphology and morphometric of the mare reproductive organs. The ovarium was fixed in 4% paraformaldehyde solution then followed by histological method and stained using hematoxylin and eosin (HE) and Masson’s trichome (MT). Three mares were observed for diameter and changes overview of uterus during estrous cycle in real time using ultrasound. The results showed that, in general, the anatomy of Gayo mare’s reproductive organs was similar to other mares, but smaller in morphometry. The total length of the Gayo mare’s reproductive tract from labia to apex cornua was 48.00±1.00 cm. Weight of Gayo mare’s left ovary was 19.07±7.70 g and the right was 24.43±0.83 g. Histologically, there was no difference between Gayo mare’s structure and other mares. In cortex uteri there were some follicles surrounded by capillary, various development stages of follicles, healthy follicles, atretic follicle, and corpus albican; while in medulla there were a lot of connective tissues. Ultrasound of the uterus showed the change in diameter during estrous cycle with the largest diameter of corpus uteri was 4.43±0.10 cm in horses with estrous cycle of 21 days and 6.30±0.93 cm in horses with 24 days estrous cycle. In conclusion, the morphometry of Gayo mare reproductive organs are smaller than the other horses and there are differences in diameter of the uterus during the estrous cycle due to the changes of endometrium thickness.

Co-Authors A Amrozi Abdul Samik Adji, Vita Adonia Afiff , Usamah Ahmada, Milla Alwi, Alwi Shobri Amala, Karisma Wahyu Amarudin, Amin Awal Amrozi Amrozi, A Andre Pasetha Anriansyah Renggaman Antje Engelhardt Antje Engelhardt Antje Engelhardt Antje Engelhardt, Antje Asmawan, Yudhi Ferdi Andri Asri Pudjirahaju Bakti Tamba Bambang Purwantara Berlin Pandapotan Pardede Borman, Mohammad Riski Cece Sumantri Dandan Haryono Dea Indriani Astuti DEDI CANDRA Dedi Rahmat Setiadi Desy Desy, Desy Dilla frastantie Dondin Sajuthi DYAH PERWITASARI -FARAJALLAH Entang Iskandar Fathur Rahman Fitriani, Lia Gholib Gholib Gholib Gholib, Gholib GONO SEMIADI Gono Semiadi Gusman, Khalis Talitha Hamidudin, Ahmad Yahya Hamny Sofyan Harianto Budi Rahardjo Heistermann, Michael Hermansyah, Endang HUSNUL KHOTIMAH I Ketut Karya Wisana Idawati Nasution Iis Afriantini Iman Supriatna Intam Kurnia Islachiyana, Rifda Jansen Manansang Jansen Manansang Juli Melia Juli Melia Juli Melia Kamal, Lutfhi Annisa Ketut Adnyane Mudite koekoeh santoso La Ode Muhammad Aswad Salam Lidya Elizabeth M. Manik Lisa Michelle Danish M Agus Setiadi Mahmudi Mahmudi Marniati Kadir Michael Heistermann Minarti, Melisa Muchidin Noordin Muhammad Fathoni, Muhammad Muhammad Jalaluddin Muhammad Subhan Muhammad Syawal Muhammad Syawal Muslim Akmal Mustainah Mappatoba Nanik Hidayatik Ni Wayan Kurniani Karja Nisa, Hairun Nisa Pardede, Berlin Pandapotan Putra, Saiful Aminudin Al Kusuma R. Iis Arifiantini R. Taufiq Purna Nugraha R. Taufiq Purna Nugraha Ridzky Pratama Sartika Lina Mulani Setyaningsih Rambu Liwa Setyaningsih Rambu Liwa Sevy Tiara Ilham Sholikhah, Noviana Nur Sinta Maryam, Sinta Sri Wahyuni Sri Wahyuni Srihadi Agungpriyono Surya Agus Prihatno Sutrisnak Sutrisnak Syahruddin Said Taufiq P. Nugraha Taufiq Purna Nugraha, Taufiq Purna Teguh Sumarsono Tuty L Yusuf Tuty L Yusuf Tuty L Yusuf Tuty L. Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf TUTY LASWARDI YUSUF Tuty Laswardi Yusuf Tuty Laswardy Yusuf Vincentia Trisna Yoelinda Wahono Esthi Prasetyaningtyas Wahyuni, Sri Yohana Tri Hastuti Yohana Tri Hastuti Yosua Kristian Adi Zunaidi, Arif

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