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All Journal HAYATI Journal of Biosciences ACTA VETERINARIA INDONESIANA Hemera Zoa Media Veteriner Jurnal Sain Veteriner Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter BIOTROPIA - The Southeast Asian Journal of Tropical Biology ZOO INDONESIA ARSHI Veterinary Letters Dinamika Informatika: Jurnal Ilmiah Teknologi Informasi Indonesian Journal of Applied Research (IJAR) PARADIGMA Borneo Journal of Science and Mathematics Education Borneo Journal of Primary Educaton Al-Muraqabah: Journal of Management and Sharia Business Jurnal Kedokteran Hewan Jurnal Kajian Ekonomi dan Manajemen Indonesia Policy Brief Pertanian, Kelautan, dan Biosains Tropika Al-Insyiroh: Jurnal Studi Keislaman Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Tarbiyah Jamiat Kheir Research Economics Management and Business Jurnal Bioteknologi & Biosains Indonesia
MUHAMMAD AGIL
Bagian Reproduksi Dan Kebidanan Departemen Klinik Reproduksi Dan Patologi Fakultas Kedokteran Hewan Institut Pertanian Bogor, Bogor
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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COMPARATIVE ASSESSMENT OF BULL SPERM MOTILITY AND CONCENTRATION: CONVENTIONAL METHODS VERSUS PORTABLE ANDROSCOPE CASA SYSTEM Arifiantini, Raden Iis; Gusman, Khalis Talitha; Agil, Muhammad; Karja, Ni Wayan Kurniani
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10400

Abstract

Operational implementation, such as laboratory staff and equipment, differ for each frozen semen producer, resulting in variability in the quality assessment of the frozen semen produced. Conventional assessments subject to subjectivity, human error, and high variability. Computer-assisted semen analysis (CASA) is considered more objective. This study aimed was to conduct a comparative analysis of motility and concentration assessment of frozen semen sperm using conventional and the portable CASA AndroScope. Twenty-one laboratory assistants from 21 national and regional frozen semen producers participated in this study. Sperm motility and concentration were assessed conventionally (using a Neubauer chamber) and AndroScope was used with five replicates. The results of the comparison of sperm motility scores revealed significant differences (p<0.05) between conventional examination and using AndroScope. The results of the comparison of the sperm concentration calculations revealed no significant difference (p>0.05) between the calculations performed using the Neubauer chamber and AndroScope. Assessment with the AndroScope is considered reliable and can replace conventional assessment in assessing motility and sperm concentration, thereby improving and standardizing the assessment performed by frozen semen producers.
COMPARATIVE ASSESSMENT OF BULL SPERM MOTILITY AND CONCENTRATION: CONVENTIONAL METHODS VERSUS PORTABLE ANDROSCOPE CASA SYSTEM Arifiantini, Raden Iis; Gusman, Khalis Talitha; Agil, Muhammad; Karja, Ni Wayan Kurniani
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10400

Abstract

Operational implementation, such as laboratory staff and equipment, differ for each frozen semen producer, resulting in variability in the quality assessment of the frozen semen produced. Conventional assessments subject to subjectivity, human error, and high variability. Computer-assisted semen analysis (CASA) is considered more objective. This study aimed was to conduct a comparative analysis of motility and concentration assessment of frozen semen sperm using conventional and the portable CASA AndroScope. Twenty-one laboratory assistants from 21 national and regional frozen semen producers participated in this study. Sperm motility and concentration were assessed conventionally (using a Neubauer chamber) and AndroScope was used with five replicates. The results of the comparison of sperm motility scores revealed significant differences (p<0.05) between conventional examination and using AndroScope. The results of the comparison of the sperm concentration calculations revealed no significant difference (p>0.05) between the calculations performed using the Neubauer chamber and AndroScope. Assessment with the AndroScope is considered reliable and can replace conventional assessment in assessing motility and sperm concentration, thereby improving and standardizing the assessment performed by frozen semen producers.
BIOLOGICAL CHARACTERISTIC OF INDONESIAN GAYO HORSE Melia, Juli; Amrozi, Amrozi; Agil, Muhammad; Supriatna, Iman
BIOTROPIA Vol. 29 No. 1 (2022): BIOTROPIA Vol. 29 No. 1 April 2022
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2022.29.1.834

Abstract

This study aims to find out the characteristic of gayo horse and to identify the population of gayo horse. Data collection was conducted through selection process over 100 local horse distributed in Central Aceh Sub-districts, Gayo Lues and Bener Meriah. The result was 30 gayo horses with varying age distribution. Detail observation was performed on six gayo horses of male (n=3) and female (n=3). The observation consisted of morphological observation and morphometry. Data collection of gayo horse population was based on annual report from local Animal Husbandry and Fishery Official over the last 5 years. Result showed that gayo horse has straight cranial shape similar to thoroughbred with smaller size, thick and stiff mane, and ears akin to donkey. Gayo horse body height ranges between 113-120 with body weight ranging around 215-280 kg. Gayo horse is agile despite living in mountain and able to carry heavy weight. There has been drastic decline of population in year 2010-2014. In conclusion, gayo horse is included as large pony category, and without any rescue effort, gayo horse is predicted to be extinct in 2037.
Longevity and Recovery Rate of Indonesian Local Ram Spermatozoa in Homemade and Commercial Extenders Kamal, Lutfhi Annisa; Agil, Muhammad; Said, Syahruddin; Nugraha, Taufiq Purna; Arifiantini, Raden Iis
Indonesian Journal of Applied Research (IJAR) Vol. 7 No. 1 (2026): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v7i1.827

Abstract

Sheep farming plays a crucial role in Indonesia's livestock industry; however, the effectiveness of artificial insemination (AI) is hindered by the less-than-ideal quality of cryopreserved semen. This research assessed the longevity and progressive motility recovery rate (RR) of frozen semen from local Indonesian rams, using a homemade Tris egg yolk (TEY) extender and three commercial extenders (AndroMed®, Steridyl®, BoviFree®). Semen from three rams (Batur, Garut, and Sakub) was processed, frozen in 0.25 mL straws (50 × 10⁶ sperm per straw), and its quality was evaluated using CASA. Longevity was monitored hourly from 0 to 4 h at 37°C. The data were analyzed using a Randomized Block Design with ANOVA, followed by Duncan’s Multiple Range Test. Sperm motility decreased over incubation time for all extenders, with notable differences at 4 h (total motility, p = 0.017; progressive motility, p = 0.023). Commercial extenders preserved higher total and progressive motility than TEY, with Steridyl® showing the highest mean RR (64.11%; mean all rams; RR analysis, n = 18), significantly outperforming Andromed®, BoviFree®, and TEY (p = 0.007). The superior results of Steridyl® align with its low-density lipoprotein content, which stabilizes the sperm membranes during cryopreservation. In summary, Steridyl® was the most effective extender for maintaining motility and RR of frozen semen from local Indonesian rams, potentially enhancing AI success in small ruminant programs.
Co-Authors A Amrozi Abdul Samik Adji, Vita Adonia Afiff , Usamah Ahmada, Milla Alwi, Alwi Shobri Amala, Karisma Wahyu Amarudin, Amin Awal Amrozi Amrozi, A Andre Pasetha Anriansyah Renggaman Antje Engelhardt Antje Engelhardt Antje Engelhardt Antje Engelhardt, Antje Asmawan, Yudhi Ferdi Andri Asri Pudjirahaju Bakti Tamba Bambang Purwantara Berlin Pandapotan Pardede Borman, Mohammad Riski Cece Sumantri Dandan Haryono Dea Indriani Astuti DEDI CANDRA Dedi Rahmat Setiadi Desy Desy, Desy Dilla frastantie Dondin Sajuthi DYAH PERWITASARI -FARAJALLAH Entang Iskandar Fathur Rahman Fitriani, Lia Gholib Gholib Gholib Gholib, Gholib Gono Semiadi GONO SEMIADI Gusman, Khalis Talitha Hamidudin, Ahmad Yahya Hamny Sofyan Harianto Budi Rahardjo Heistermann, Michael Hermansyah, Endang HUSNUL KHOTIMAH I Ketut Karya Wisana Idawati Nasution Iis Afriantini Iman Supriatna Intam Kurnia Islachiyana, Rifda Jansen Manansang Jansen Manansang Juli Melia Juli Melia Juli Melia Kamal, Lutfhi Annisa Ketut Adnyane Mudite koekoeh santoso La Ode Muhammad Aswad Salam Lidya Elizabeth M. Manik Lisa Michelle Danish M Agus Setiadi Mahmudi Mahmudi Marniati Kadir Michael Heistermann Minarti, Melisa Muchidin Noordin Muhammad Fathoni, Muhammad Muhammad Jalaluddin Muhammad Subhan Muhammad Syawal Muhammad Syawal Muslim Akmal Mustainah Mappatoba Nanik Hidayatik Ni Wayan Kurniani Karja Nisa, Hairun Nisa Pardede, Berlin Pandapotan Putra, Saiful Aminudin Al Kusuma R. Iis Arifiantini R. Taufiq Purna Nugraha R. Taufiq Purna Nugraha Ridzky Pratama Sartika Lina Mulani Setyaningsih Rambu Liwa Setyaningsih Rambu Liwa Sevy Tiara Ilham Sholikhah, Noviana Nur Sinta Maryam, Sinta Sri Wahyuni Sri Wahyuni Srihadi Agungpriyono Surya Agus Prihatno Sutrisnak Sutrisnak Syahruddin Said Taufiq P. Nugraha Taufiq Purna Nugraha, Taufiq Purna Teguh Sumarsono Tuty L Yusuf Tuty L Yusuf Tuty L Yusuf Tuty L. Yusuf TUTY LASWARDI YUSUF Tuty Laswardi Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf Tuty Laswardy Yusuf Vincentia Trisna Yoelinda Wahono Esthi Prasetyaningtyas Wahyuni, Sri Yohana Tri Hastuti Yohana Tri Hastuti Yosua Kristian Adi Zunaidi, Arif