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Novia Novia
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Journal : Journal of Integrated and Advanced Engineering (JIAE)

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Enzymatic hydrolysis of cellulose banana stem (alkaline microwave-assisted pre-treatment) Samara, Faras Saskia; Novia, Novia; Melwita, Elda
Journal of Integrated and Advanced Engineering (JIAE) Vol 4, No 1 (2024)
Publisher : Akademisi dan Saintis Indonesia (ASASI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51662/jiae.v4i1.120

Abstract

The banana stem waste holds immense promise as a readily available and abundant source of lignocellulosic biomass, making it a compelling alternative for biofuel and biochemical applications. Therefore, this study focuses on investigating the impact of both time and substrate loading on the enzymatic hydrolysis of banana stem cellulose that has undergone alkaline microwave-assisted pre-treatment. The pre-treatment method involves subjecting the biomass to 5% KOH for 30 min, followed by microwave exposure at 300 W for 5 min, a process aimed at enhancing the accessibility of cellulose. Enzymatic hydrolysis experiments were carried out utilizing cellulase enzymes derived from Aspergillus niger, with variations in hydrolysis times (ranging from 5 to 45 h) and enzyme-to-substrate ratios (ranging from 1:1 to 1:10). The results of this investigation revealed a substantial improvement in hydrolysis efficiency, owing to the synergistic effects of alkaline microwave-assisted pre-treatment, signifying enhanced cellulose accessibility. Notably, the highest concentration of reducing sugars (1.3 mg mL−1) was achieved at a substrate-to-enzyme ratio of 1:1 and a hydrolysis duration of 45 h. These findings provide valuable insights into the conversion of lignocellulosic biomass, emphasizing the potential of integrated pre-treatment strategies for sustainable biorefinery applications. This research contributes to advancing our understanding of lignocellulosic biomass utilization, offering a promising avenue for biofuel and biochemical production from banana stem waste.
Co-Authors . Elvitriana . Ermanto Adrianto, Fajri Agustien , Anthoni Ahmad Nusi Al Muttaqii, Muhammad Alya Rahmaditya Arfan Amallia Putri Amelia, Ririn Andi Bungawati, Andi Anggriani, Ulfa Meila Anugrah Wati, Luki Anugrah, Muhammad Risky Aprianti, Tine Ardila, Ardila Arfan , Alya Rahmaditya ARINAFRIL ARINAFRIL Aryani, Mentari Dian Asep Eka Nugraha, Asep Eka Asri, Wan Ryan Azhari, Muhamad Thohir Bahagia Bahagia Bazlina Dawami Afrah Belinda, Michelle Daimun, Daimun David Bahrin Deasy Ovi Harsachatri Desven Hecca, Desven Dewirahmadanirwati, Dewirahmadanirwati Difa Intannia Erwiantono Fadila Harisi, Fitria Fahira, Nurul Savira Fahma Riyanti Fitri Hadiah Habibi , Alif Rahman Halida Hambali, Daimun Harisi, Fitria Fadila Harsachatri , Deasy Ovi Hasanudin Hasanudin Havid Ardi Hermansyah Hermansyah Herningtyas Nautika Lingga Huwaidah, Wulan Ariqah Idris Idris Ira Geraldina Kasmiarti, Getari Larasati , Julia Veronika Lestari, Ade Ayu Lia Milana, Lia Lovelytan, Fidelia Lutfin, Nursamsilis Maryana, Roni Mas'ud Muhammadiah Maulin Wiraningsih, Maulin Melwita, Elda Mifthahul Jannah Mifthahul Jannah Muhammad Faisal Muhammad Faizal Muhammad Khairul MUHAMMAD SAID Mulyadi, Akbar Nakashima, Mhika Napiah Nasution, Ali Nino Rinaldi Nuraina, Nuraina Nurhayati Nurhayati Nurlaili , Nurlaili Nurlaili Nurlaili Nurul Fajri Saminan, Nurul Fajri Nusi , Ahmad Pangestika , Yuliandini Permatasari, Rindah Poedji Loekitowati Hariani Pramita Sylvia Dewi Prastyawan, Riyani Pratiwi, Astrid Siska Putri, Riska Dwi Putri, Tiara Maharani Ramona Qoriah Saleha Radjagukguk, Djudjur Luciana Rahmat, Karel Gusmin Rahmawati, Rahmawati Raihan, Abiyandra Radika rendana, muhammad Rida, Cut Nova Rosnilawati Rosnilawati Samara, Faras Saskia Saputri, Jasmine Fadhilah Delli Selpiana, Selpiana Septa Liana Shailendra, Faris Habib Shean, Rizqi Akbar Sonia Sonia, Sonia Suhartina Suhartina, Suhartina Suhendrayatna Suhendrayatna Supangadi, Egita Riyanti Supriyadi Supriyadi Susi Susanti Syafi’i, Syafi’i TATI NURHAYATI Trezenki, Randa Tuti Indah Sari, Tuti Indah Uli Sitanggang, Cesilia Sondang Wijaya, Ali Satria Wirawan, Fandi Surya Yazila, Syukran Yuhelna . Yulandari, Septya Yumi Lindawati Yunia Wardi Yuniarti, Hafizhah Yunisrul Yunisrul z, rohantizani Zuardi, Zuardi