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All Journal JURNAL PESISIR DAN LAUT TROPIS JURNAL ILMIAH PLATAX JURNAL PERIKANAN DAN KELAUTAN TROPIS JOURNAL OF COASTAL DEVELOPMENT Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati ZOO INDONESIA Bawal : Widya Riset Perikanan Tangkap Zoo Indonesia Devotion: Journal of Research and Community Service Indonesian Journal of Mollusk EKOTON
Medy Ompi
Faculty Of Fisheries And Marine Science, Sam Ratulangi University, Manado, 95115, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Other

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The Effect of Patch Size on Morphology and Growth on The Intertidal Box Mussel Septifer bilocularis L., in North Sulawesi, Indonesia Medy Ompi; Lawrence J.L. Lumingas
Jurnal Moluska Indonesia Vol. 3 No. 1 (2019): April 2019
Publisher : Masyarakat Moluska Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54115/jmi.v3i1.33

Abstract

Isolated small and large patches of Septifer bilocularis were divided into three length size classes: small (>10 to 17 mm), medium (>17 to 23 mm), and large (>23 mm) mussels. Each group was divided into three groups of different densities to form isolated patches (10 individuals), medium patches (50 individuals), and large patches (100 individuals). Three replicates of each patch size were established. Each density group was placed in a cage and returned to their natural hard bottom substrata, placed randomly, 50 cm between cages. At low water level, the area was exposed to air. The length / weight regression line of mussels in small and large patches overlapped, but there was a significant difference between the slopes (ANCOVA, p<0.05). Mussels occurring in small patches were heavier and thicker than those of larger patches. Box mussels in small patches had significantly higher growth than mussels in medium and large patches
Rekrutmen Box Mussel, Septifer Bilocularis L. Efek Substratum Dan Kepadatan Dewasa Medy Ompi
Jurnal Moluska Indonesia Vol. 3 No. 2 (2019): Oktober 2019
Publisher : Masyarakat Moluska Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54115/jmi.v3i2.34

Abstract

An intertidal population of Septifer bilocularis L. was studied in Tongkeina, North Sulawesi, Indonesia. Open and closed cages were applied in field experiments to study the effects of four types of substrata on settling of S. bilocularis: live shells of adults, dead shells, coral rubble, and sand. Each substratum was tested with four levels of adult mussel density: 0, 10, 50, 100 individuals per compartment. The number of recruits as a function of cage, substratum, and adult density was analysed using Two-way ANOVA. The experiment showed that new recruits were affected by the substratum but not by the density of adults. The surface of adult box mussels was the most attractive substratum for recruits
LARVA AVERTEBRATA LAUT DI AIR "BALLAST" DAN PERAIRAN LAUT DI PELABUHAN BITUNG, SELAT LEMBE, SULAWESI UTARA Medy Ompi
ZOO INDONESIA Vol 19, No 1 (2010): Juli 2010
Publisher : Masyarakat Zoologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52508/zi.v19i1.2392

Abstract

Penelitian larva
PENGGUNAAN DNA BARCODING DALAM MENGIDENTIFIKASI LARVA GASTROPODA (FAMILY CYMATIIDAE) DI PERAIRAN KEPULAUAN SANGIHE- TALAUD, SULAWESI UTARA Anna Rejeki Simbolon; Medy Ompi; Ernawati Widyastuti; Diah Anggraini Wulandari
BAWAL Widya Riset Perikanan Tangkap Vol 13, No 3 (2021): (DESEMBER) 2021
Publisher : Pusat Riset Perikanan, BRSDM KP.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/bawal.13.3.2021.%p

Abstract

Perairan Kepulauan Sangihe-Talaud merupakan wilayah pemijahan hewan laut, sehingga berbagai jenis larva hewan laut dapat ditemukan di wilayah ini. Identifikasi larva gastropoda secara morfologi sangat sulit dilakukan karena tingginya kesamaan morfologi antar spesies pada fase larva. DNA Barcoding dapat digunakan untuk mengidentifikasi larva berbagai hewan laut. Penelitian ini bertujuan untuk mengidentifikasi larva gastropoda dari Family Cymatiidae di Perairan Kepulauan Sangihe-Talaud. Sampel larva diambil dengan menggunakan alat Issaac Kid Midwater Tramp (IKMT) di kedalaman 1000 m di bawah permukaan laut pada siang dan malam hari. Barcoding DNA menggunakan primer universal LCO 1490 dan HCO 2198, pengeditan dan alignment sekuen DNA dalam Geneious ver. 9 dan identifikasi menggunakan BLAST di GenBank NCBI. Pohon filogenetik dikonstruksi menggunakan metode neighbor joining (NJ) dan model Kimura-2i dalam MEGA X. Sebanyak 39 spesimen larva Cymatiidae terdiri atas 6 genus yaitu Reticutriton, Monoplex, Turritriton, Cymatium, Gutturnium, dan Ranularia; lima spesies teridentifikasi: Reticutriton pfeifferianus, Monoplex aquatilus, Monoplex comptus, Cymatium cingulatum dan Gutturnium muricinum, dengan tingkat kesamaan 97,32-100%. Berdasarkan pohon filogenetik sebagian sekuen individu termasuk kelompok monofiletik sedangkan genus Monoplex bersifat polifiletik dan mengelompok dalam genus yang berbeda dengan nilai boostrap yang cukup tinggi. Jarak genetik antar spesies berkisar 0,0761-0,1737 dengan jarak genetik antar spesies terendah pada Monoplex comptus dan Reticutricon pfeifferianus. Penelitian ini menyimpulkan DNA barcoding dapat digunakan dalam mengidentifikasi larva Gastropoda khususnya family Cymatiidae. Identifikasi jenis hewan laut secara dini menjadi salah satu kunci penting dalam pengelolaan lestari jenis hewan laut. Identifikasi larva yang tepat akan berguna dalam pengelolaan konservasi suatu wilayah perairan.The seas around the Sangihe-Talaud Islands are a spawning ground for marine animals, so many marine animals' larva can be found in this area. Morphological identification of gastropod larvae is complicated because of the high morphological similarities between species in the larval stage. DNA Barcoding can be used to identify the larvae of various marine animals. This study aims to identify gastropod larvae, especially the family Cymatiidae, in the waters around the Sangihe-Talaud Islands. Larvae samples were collected using an Isaac Kid Midwater Tramp (IKMT) at a depth of 1000m below sea level day and night. DNA barcoding used the universal primers LCO 1490 and HCO 2198. DNA sequences were edited and aligned in Geneious ver nine and identified using the NCBI GenBank BLAST routine. A phylogenetic tree was constructed using the neighbor-joining (NJ) method with the Kimura-2 model. 39 Cymatiidae larvae were identified as belonging to 6 genera: Reticutriton, Monoplex, Turritriton, Cymatium, Gutturnium, and Ranularia; five species were identified: Reticutriton pfeifferianus, Monoplex aquariums, Monoplex computers, Cymatium cingulated and Gutturnium muricinum, with a similarity 97.32-100%. Based on the phylogenetic tree, some of the individual sequences belong to monophyletic groups, while the Monoplex genus is polyphyletic and grouped into several genera with relatively high bootstrap values. The genetic distance between species ranged from 0.0761-to 0.1737 with the lowest inter-species genetic distance between Monoplex computers and Reticutricon pfeifferianus. This study concludes that DNA barcoding can be used to identify gastropod larvae, in particular the family Cymatiidae. Early identification of marine animal species is an important key to the sustainable management of marine animals. Identification of gastropod larvae can support the conservation management of marine waters.
GASTROPOD COMMUNITY STRUCTURE in the WATERS of GANGGA SATU VILLAGE, WEST LIKUPANG, NORTH MINAHASA Klaudio Mauli; Alex D Kambey; Ruddy D Moningkey; Nego E Bataragoa; Jety K Rangan; Medy Ompi
JURNAL PERIKANAN DAN KELAUTAN TROPIS Vol. 11 No. 2 (2022): EDISI MEY-AGUSTUS 2022
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jpkt.12.1.2021.42509

Abstract

This study aims to determine the types and community structures of gastropods in the waters of Gangga Satu Village, West Likupang, North Minahasa. Data were collected using the line transect quadratic method, with a transect length of 100 m and a square measure of 1 m x 1 m. Gastropod species in the southern waters of Gangga Satu village, West Likupang, North Minahasa consist of 18 species (11 genera), 5 families, and 4 orders.  namely Thais tuberosa, Morula rumphius, Nassarius distorus, Nassarius albescens, Mitra litterata, Mitra paupercula, Conus parvulus, Conus striatus, Conus Chaldeaus, Engina mendicaria, Nerita undata, Nerita plicata, Nerita polita, Clypeomorus cerithium acina, Turbo bruneus. Density of all species K = 4.76, relative density of 13% in Morula rumphius species, diversity of H' = 1.18 and dominance of C = 0.07Keywords: Community structur; Gastropod AbstrakPenelitian ini bertujuan untuk mengetahui jenis-jenis dan struktur komunitas gastropoda di Perairan Desa Gangga Satu Likupang Barat Minahasa Utara. Pengambilan data menggunakan metode line transek kuadrat , dengan panjang transek 100 m dan ukuran kuadrat 1m x 1m. Jenis Gastropoda  di perairan selatan desa Gangga Satu, Likupang Barat, Minahasa Utara terdiri dari 18 spesies (11 genus), 5 famili, dan 4 ordo  yakni Thais tuberosa, Morula rumphius, Nassarius distorus, Nassarius albescens, Mitra litterata, Mitra paupercula, Conus parvulus, Conus striatus, Conus Chaldeaus, Engina mendicaria, Nerita undata, Nerita plicata, Nerita polita, Clypeomorus bifasciata, Cerithium atratum, Lunela cineria, Turbo bruneus. Kepadatan seluru spesies K= 4,76, kepadatan relatif sebesar 13% pada spesies Morula rumphius, keanekaragaman H’= 1,18 dan dominasi C= 0,07Kata kunci:Strukturkomunitas; Gastropoda
PERAN KERANG PENYARING PARTIKEL TERHADAP KERAGAMAN MAKROBENTHOS DI AGGREGASI KERANG Medy Ompi; Farnis B. Boneka; Erly Y. Kaligis; Stella T. Kaunang
BAWAL Widya Riset Perikanan Tangkap Vol 15, No 1 (2023): (APRIL) 2023
Publisher : Politeknik Kelautan dan Perikanan Sorong

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/bawal.15.1.2023.1-13

Abstract

Tujuan penelitian ini adalah untuk mengetahui distribusi jenis, kelimpahan, keragaman, dan kesamaan makrobenthos yang berada di dalam posisi aggregasi di lokasi berbeda.  Observasi telah dilakukan di 2 stasion Tiwoho dan Blongko, selama April, 2021. Makrobentos diambil dengan meletakan ‘Core’ (PVC) dengan diameter 15 cm pada posisi tengah dan pinggir aggregasi besar, serta aggregasi kecil di 2 lokasi.  Materi yang ada di dalam ‘Core’ diangkat, dimasukkan di dalam plastik yang berlabel, selanjutnya disortir, diidentifikasi, dihitung, dan foto di laboratorium.  Pengambilan sampel dilakukan sebanyak 4 kali sebagai ulangan pada posisi aggregasi yang berbeda.  Jumlah jenis dan individu makrobenthos dianalisa dengan menggunakan 2 Arah ANOVA, di mana posisi dalam aggregasi dan stasion adalah sebagai faktor utama.  Mengetahui keragaman dilakukan melalui perhitungan Indeks ‘Simpon’ dan kesamaan dengan Indeks ‘Evenness’.  Hasil menunjukkan bahwa jumlah jenis dan jumlah individu (kelimpahan) makrobenthos bervariasi di antara posisi dalam aggregasi kerang, namun tidak dipengaruhi oleh baik posisi dalam aggregasi dan stasion.  Keragaman dan kesamaan jenis makrobenthos dikategorikan tinggi, walaupun baik ‘Indeks Simpson’ dan ‘Evenness’ bervariasi dan tidak konsiten di antara posisi dalam aggregasi dan stasion.  Jumlah individu (kelimpahan) makrobenthos nampak bebeda di antara posisi aggregasi di lokasi Blongko berdampak pada perbedaan stabilitas makrobenthos untuk kedua lokasi. 
Implementation of Environmentally Friendly Monitoring Method: "Green Watch" and "Image Analysis" in Sustainable Resource Utilization in the Coast Community of the Gulf of Amurang, North Sulawesi Fontje Georis Judri Kaligis; Medy Ompi
Jurnal Ilmiah PLATAX Vol. 4 No. 2 (2016): EDISI JULI-DESEMBER 2016
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.4.2.2016.14204

Abstract

Monitoring the condition of coral reefs using 'green watch' and 'image analysis' in the use of sustainable resource in the coastal communities of the Gulf of Amurang North Sulawesi has been conducted with the purpose of (1) to understand the condition of coral reefs in the waters of TEEP and Kapitu, (2) the coastal communities can utilize the method of 'green watch' and 'image analysis', and also can do the monitoring themselves both groups and individuals. Results of research by two methods illustrates that there are seven types of coral growth in the waters of Teep, and 8 types in the waters Kapitu village, where each type of coral growth showed different in percent cover. Overall reef condition for waters in the village of TEEP is very good with 79% of live coral, while the  Kapitu village is good with a percentage of live coral is 64%. With this condition is expected that the community can do the monitoring continuously, and also rehabilitate the damage corals. By doing so the resources will be always available for both village.   Keywords: Coral Reef, associate organisms, 'green watch', 'image analysis', live coral  and dead coral, percent cover, Abstrak Monitoring kondisi terumbu karang dengan menggunakan metode ‘green watch’ dan ‘ image analysis’ dalam pemanfaatan sumberdaya yang berkelanjutan di komunitas pesisir Teluk Amurang Sulawesi Utara telah dilakukan dengan tujuan (1) memahami kondisi terumbu karang di perairan Teep dan Kapitu, (2) masyarakat nelayan dapat memanfaatkan metode ‘green watch’ dan ‘image analysis’, sekaligus melakukan monitoring secara mandiri baik kelompok maupun individu.  Hasil penelitian dengan dua metode ini menggambarkan bahwa ada 7 tipe pertumbuhan karang di desa Teep, dan 8 tipe di perairan desa Kapitu, di mana masing masing tipe pertumbuhan karang hadir dengan presentase tutupan yang berbeda.  Kondisi karang secara keseluruhan untuk perairan di Desa Teep adalah yang sangat bagus dengan 79 % karang hidup, sedang untuk desa Kapitu adalah cukup bagus dengan presentase karang hidup adalah 64 %.  Dengan kondisi ini diharapkan masyarakat dapat mempertahankan, dengan cara melakukan kegiatan monitoring secara berkesinambungan, sekaligus merehabilitasi kondisi karang yang telah rusak. Dengan  mempertahankan kondisi yang baik ini maka servis dari ekosistem ini terhadap masyarakat di kedua Desa ini akan terus ada.   Kata kunci: Terumbu Karang, organisme asosiasi, ‘green watch’, ‘image analysis’, karang hidup, karang mati, persentase tutupan.
Shell Color Classification And Carotenoid Pigments On Littoraria pallescens (Philippi, 1846) From Mangrove Ecosystem Area On Mokupa Village, Tombariri Sub District and Basaan Village, Ratatotok Sub District) Susan M. Sumampouw; Desy M. H. Mantiri; Farnis B. Boneka; Medy Ompi; James J. H. Paulus; Adnan S. Wantasen
Jurnal Ilmiah PLATAX Vol. 6 No. 2 (2018): ISSUE JULY-DECEMBER 2018
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.6.2.2018.20650

Abstract

The purpose of this study was to classify the color of the shell and to know the carotenoid pigment content in Littoraria pallescens based on color classification and population distribution in the mangrove ecosystem area of Mokupa Village, Tombariri Sub district and Basaan Village, Ratatotok Sub district. Sampling directly on mangrove trees as water begins to recede. Identification of L. pallescens species is done by looking at the shape of the shell, the color of the shell, the color of the operculum and the shape of the genital organ. Shell color classification by inserting into the Color Explorer application. Analysis of carotenoid pigments by extraction process with acetone and petroleum ether, further separation of pigment by thin layer chromatography. The results obtained show that L. pallescence dominates life on mangrove trees. Sex was inversely proportional to the two research sites, 53.8% of the male L. pallescens species and 46.2% female in Mokupa waters while from Basaan waters there were 47.1% males and 52.9% females. Color classification based on the percentage of occurrences of constant color that is black (18.5), black orange (16.3) brown black spots (16,3), gray (10.7), angry (6,3), yellow pale (17.8), brown yellow spots (14,1). The detected pigment based on the color classification of the shell is located on the identical and identifiable Rf for all colors is the β-carotene pigment.Keyword : Littoraria pallescence, Carotenoid pigments Tujuan dari penelitian ini adalah untuk mengklasifikasikan warna cangkang dan mengetahui kandungan pigmen karotenoid pada Littoraria pallescens berdasarkan klasifikasi warna dan sebaran populasinya di wilayah ekosistem mangrove Desa Mokupa kecamatan Tombariri dan Desa Basaan Kecamatan Ratatotok. Pengambilan sampel secara langsung pada pohon mangrove saat air mulai surut. Identifikasi spesies L. pallescens dilakukan dengan melihat bentuk cangkang, warna cangkang, warna operculum dan bentuk organ genital.  Pendataan untuk klasifikasi warna cangkang dengan memasukkan ke dalam aplikasi Color Explorer. Analisis pigmen karotenoid melalui proses ekstraksi dengan aseton dan petroleum eter, selanjutnya pemisahan awal pigmen dengan kromatografi lapis tipis. Hasil yang diperoleh menunjukan bahwa L. pallescence mendominasi hidup pada pohon mangrove. Jenis kelamin berbanding terbalik pada dua lokasi penelitian, spesies L. pallescens jantan 53.8% dan betina 46.2% di perairan Mokupa sedangkan dari perairan Basaan terdapat 47.1% jantan dan 52.9% betina. Klasifikasi warna berdasarkan persentase kemunculan warna yang konstan yaitu warna hitam (18,5), hitam oranye (16,3) coklat bercak hitam (16,3), abu-abu (10,7), Marah (6,3), kuning pucat (17,8), kuning bercak coklat (14,1). Pigmen yang terdeteksi berdasarkan klasifikasi warna pada cangkang adalah berada pada Rf yang sama dan yang dapat diidentifikasi untuk semua warna adalah pigmen ß-karoten.Kata kunci : Littoraria pallescence, Pigmen Karotenoid
Foraminifera On The Beach Of Malalayang Dua Petrick Billy; Jane M. Mamuaja; Royke M. Rampengan; Medy Ompi; Esry T. Opa; Joppy Mudeng
Jurnal Ilmiah PLATAX Vol. 6 No. 2 (2018): ISSUE JULY-DECEMBER 2018
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.6.2.2018.20660

Abstract

Beach as one on the landform that reflects the work of hydro-oceanographic factors, generally in the form of loose sedimentary material. Loose sediment is a collection of organic and inorganic particles that accumulate widely and are irregular in shape. One example of organism in marine waters that contributes to the availability of organic particles in the beach landform is foraminifera. Foraminifera is a single-celled organism that has the ability to form shells from substances of CaCO3 which originate from itself or from the surrounding environment. This study was aimed to classify physical sediments on the Malalayang Dua beach according to the points of sediment sampling, and see how the composition of foraminifera in the beach area and analyze the presence of foraminifera in relation to the granulometry of beach sediments. From the result of the study, it is found that the composition of grain size of sediment on the beach of Malalayang Dua is different, in stasion 1A, 1B, 2A, and 2B the sediment were mostly composed by fine grains while in station 3A and 3B were of coarse-grained sediment. A number of a species of foraminifera (dead test) was found in the study, and the number of tests was highes in fine sediments compared to coarse sediment.Keywords : Beach Landform, Malalayang Dua Coast, Foraminifera ABSTRAKGisik sebagai salah satu bentuklahan yang merefleksikan kerja faktor-faktor hidro-oseanografi, umumnya berwujud material sedimen lepas. Sedimen lepas adalah kumpulan partikel organik dan anorganik yang terakumulasi secara luas dan bentuknya tidak beraturan. Salah satu organisme di perairan laut yang berkontribusi terhadap ketersediaan partikel organik di gisik adalah foraminifera. Foraminifera merupakan organisme bersel tunggal yang mempunyai kemampuan membentuk cangkang dari zat-zat CaCO3yang berasal dari dirinya sendiri atau dari lingkungan sekitarnya. Penelitian ini bertujuan untuk mengelompokkan sedimen gisik di pantai Malalayang Dua menurut titik-titik pengambilan sampel sedimen, dan melihat bagaimana komposisi foraminifera di kawasan gisik serta menganalisis keberadaan foraminifera dalam kaitannya dengan granulometri sedimen gisik. Dari hasil penelitian komposisi ukuran butir sedimen pada lahan gisik di pantai Malalayang Dua berbeda menurut stasiun yang ditetapkan, di ruang pantai ke arah Timur yaitu stasiun 1A, 1B, 2A, 2B komposisi sedimennya berukuran halus, sedangkan di ruang pantai ke arah Barat yaitu stasiun 3A dan 3B komposisi sedimennya berukuran kasar, komposisi sedimen di setiap stasiun gisik litoral dan sublitoral menampilkan adanya perbedaan tingkat kekasaran partikel sedimen. Dari hasil penelitian ditemukan 9 cangkang foraminifera. Pada komposisi sedimen gisik yang berukuran halus ditemukan jumlah cangkang foraminifera yang lebih banyak dibandingkan dengan gisik yang komposisi sedimen berukuran kasar.Kata kunci : Lahan Gisik, Pantai Malalalayang Dua, Foraminifera
PERKEMBANGAN DAN PERILAKU PENEMPELAN LARVA GASTROPODA ABALON TROPIS Medy Ompi
EKOTON Vol. 3 No. 2 (2014): Juli - Desember 2014
Publisher : PPLH-SDA, Lembaga Penelitian Unsrat Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Abalones release gametes in the water column, where eggs are fertilized. Fertilized eggs are develop into throchophore, veliger, and pediveliger larvae. Larvae will be in the water column up to three days, then it will go down into the sea bottom to settle, metamoprhose, and developed to be juvenile and adult of abalone. A penempelan is transition phase, where mortality rate is very hight. Larvae will search for suitable substrata, where larval behaviour, substrat quality, and current are important factors. Larval abalone will refuse to settle and swim back into water column, when substrata are not suitable. Substarta such as rock, dead coral, dead and branching coral, hard substrata covered by micro algae are favourable substrata for larva abalone to settle. Larvae of marine abalones have varying in swiming speed. These larvae can be trasported by current to a long the beach and also off shore. For abalone tropis H varia, the current speed less than 5 cm per second can stimulate larva Abalone tropis such as H varia to settle.
Co-Authors Adnan S. Wantasen Alex D Kambey Andika R Balansada Angkouw, Esther D. Anna Rejeki Simbolon Antonius P Rumengan Antonius P. Rumengan Antonius Rumengan Ari B. Rondonuwu Azzahra Aulina Billy Th Wagey Billy Theodorus Wagey Boneka, Farnis B. Boneka Calvyn Calvyn Calvyn F. A. Sondak, Calvyn F. A. Calvyn, Calvyn Carolus P Paruntu Carolus Paulus Paruntu Chatrien A Sinyal Cyska Lumenta Dairivaldo, Kettang Legrant Darus S. Paransa Darwasito, Suria daud, fitran Diah Anggraini Wulandari Djamaaludin, Rignolda Ekel, Jouvan Randy Erly Y. Kaligis Erly Y. Kaligis, Erly Y. Ernawati Widyastuti Esry T. Opa F. A. Sondak Farnis B Boneka Farnis B. Boneka Farnis B. Boneka Fernando Gultom, Fernando Fontje Georis Judri Kaligis Frans Lumoindong Fransine B. Manginsela Ginting, Elvy Like Grevo S Gerung Gulo, Puji Eli Arnita Handayani, Maymanah Indri Manembu Jane M. Mamuaja Janny D. Kusen Jety K Rangan Joice R.T.S.L Rimper Joppy Mudeng Joshian N.W. Schaduw Kaligis, Early Y. Kaligis, Erly Yosef Kaligis, Georis Kasenda, Vildo Kaunang, Stella T. Kawung, Nickson Khristin I. F. Kondoy, Khristin I. F. Klaudio Mauli Kumampung, Deislie Roxmerie H. Kumentas, Veronicha Kurniati Kemer Lalita, Jans D. Lawrence J. L. Lumingas Lawrence J.L. Lumingas Lintang, Rosita A.J. Lumingas, Aaron R. T. Lumuindong, Frans Luturkey, Maureen Fenesya Mamangkey, Gustaf N. Mamangkey, Noldy G.F Mamangkey, Noldy Gustaf Frans Mamuaja, Jane M. Manaida, Frendi Manembu, Indri Shelovita Manembu Manengkey, Hermanto Wem Kling Mantiri, Desy M. H Menajang, Febry S. I. Natalie D Rumampuk Nego E Bataragoa Nickson J. Kawung, Nickson J. Nickson Kawung Ode Mantra, Syahrun Otinus Lokbere Palit, Deyti A. Pangkey, Henneke D. Pansing, Jenita Paransa, Darus Sa’adah Johanis Paringgi, Ezra Paulus, James Pelle, Wilmy E. Petrick Billy Podung, Thania Theresia Polan, Threis S. Pratasik, Silvester B. Pungus, Faldy Rampengan, Royke Rangan, Jety Rangan, Jety K. Rembet, Unstain Rene C. Kepel, Rene C. Rignolda Djamaludin Rimper, Abraham M. Rimper, Joice R. T. S. L Rimper, Joice Rinefi T.S.L Rizald Max Rompas, Rizald Max Roeroe, Kakaskasen Andreas Rose OSE Mantiri Royke M. Rampengan Ruddy D Moningkey Rumampuk, Natalie Detty C Rumengan, Antonius Petrus Rustikasari, Irna Salaki, Meiske S. Sambali, Hariyani Saragih, Hans S. R. P. Sarif Hidayat Sondak, F. A. Stella T. Kaunang Stenly Wullur Sumilat, Deiske Adeleine Suria Darwisito Susan M. Sumampouw Suzanne L Undap Tambunan, Rose Agustin Tilaar , Sandra Veibe Warouw Verisandria, Rio Winda M Mingkid