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Journal : Jurnal Veteriner

Produksi Rekombinan Bovine Lactoferrin pada Sistem Ekspresi Eschericihia coli Ni Luh Wayan Yulia Mirayanti; I Gusti Ngurah Kade Mahardika; Made Pharmawati
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (391.111 KB) | DOI: 10.19087/jveteriner.2022.23.1.105

Abstract

Lactoferrin is an 80 kDa glycoprotein which has advantages in biological activity as an antimicrobial, antibacterial, antiviral, antiparasitic, and immunomodulatory agent. Advances in recombinant technology have made it possible to produce lactoferrin proteins on a large scale, the production of recombinant lactoferrin can be carried out in various expression systems. Escherichia coli is one of the hosts in expression systems that are widely used in the production of recombinant proteins. The recombinant protein lactoferrin is produced by insertion of the bovine lactoferrin gene in the plasmid pET 11-a. The bovine lactoferrin gene that has been inserted into the plasmid pET, transformed and expressed in the cell host of E. coli BL21. The bovine lactoferrin protein expression was induced by the addition of a chaperone one of the coexpressions, which accompanied the E. coli synthesis system to achieve the desired protein expression. The expression of pET11a - Bovlacto plasmid in E. coli was supported by the inducer L-arabinose and IPTG. The bovine lactoferrin gene embedded in the recombinant plasmid pET-11a was able to be well expressed in E. coli BL21 with the hybridization signal in the dot blot method test and the target specific band, namely in the 80-85 kDa position range of SDS-PAGE electrophoresis results.
Co-Authors A. A. Putri Ardyanti A.A.S. A. Sukmaningsih Agriani Dewinta Anak Agung Ayu Putri Ardyanti Anak Agung Gde Raka Dalem Andi Bahtiar Batti Andrianus Sembiring, Andrianus Aziz, Alifya Ibnu Bayu Adjie Bayu Adjie Bayu Adjie Bram Setyadji Deny Suhermawan Yusup Dian Catur Prayantini Dwi Nur Rikhma Sari Eka Fibayani Imaniar Eka Fibayani Imaniar Ema Hendriyani Faukha Aisyatur Rahma Fransiska Nitti Fransiskus Jimmy Roga Gde Evayanti, Luh GUIJUN YAN Gusti Ayu Putri Arnila I Gde Suryadi I Gede Putu Ardhana I Gusti Agung Eka Saraswati I Gusti Ayu Agung Pradnya Paramitha I Gusti Ngurah Kade Mahardika I Ketut Catur Wiguna I Ketut Junitha I Ketut Suada I Komang Adi Widyastama I MADE AGUS WIARTANA I Made Anom Sutrisna Wijaya I Made Murna I Putu Agus Tirta Cahyana I Putu Satwika I Wayan Arnata I Wayan Rosiana I. Setyawati I.G.A.S. Wahyuni I.M.A.S. Wijaya IAN JAMES MACFARLANE IDA AYU ASTARINI Ida Ayu Ratih Purnama Dewi Ida Bagus Made Suaskara Imaniar, Eka Fibayani Indah Tria Hoky Inna Narayani IRIANI SETYAWATI Joko Raharjo, Sentot Junita Hardini Ketut Queena Fredlina Luh Putu Wrasiati Made Ria Defiani Maharani, Rai Janaki Maliza Kurnia Maya Agustina Maya Agustina Mery Wintari Ngurah Intan Wiratmini Ni Kadek Dewi Rustini NI KADEK YUNITA SARI Ni LUH ARPIWI Ni Luh Ayu Jami Wistiani, Ni Luh Ayu Jami Ni Luh Putu Agustini Ni Luh Watiniasih Ni Luh Wayan Yulia Mirayanti NI MADE DIAN PRATIWI Ni Made Gari Ni Made Sastriyani Wiendra Ni Made Sastriyani Wiendra Ni Made Suartin Ni Nyoman Wirasiti Ni Putu Adriani Astiti Ni Putu Eka Krisnayanti Ni Wayan Ayu Wiartini Ni Wayan Karolina Ni Wayan Sudatri PATRICK FINNEGAN Putera, I Kadek Aridena Putu Angga Wiradana Putu Apriliani Putu Yunita Putri Aryani Retno Kawuri Rifky Firmansyah Anwar Satwika, I Putu Septarini Dian Anitasari Septarini Dian Anitasari Siddique, Kadambot.H.M. Tassanai Jaruwatanaphan Tassanai Jaruwatanaphan Tassanai Jaruwatanaphan, Tassanai Tiwi Wati Uslan UUL SHOVI NURKAMILA Vandi Dama Setiawan Vanesa Martida Vanesa Martida Viryanando Evan Rahardja WAHYU WIDOWATI Wawan Sujarwo WENNI SETYO LESTARI Wenni Setyo Lestari Wenni Setyo Lestari, Wenni Setyo Wiguna, I Ketut Catur Wijaya, Putu Austin Widyasari Wildan Mujahidul Basyar Yasuyuki Watano Yasuyuki Watano Yasuyuki Watano, Yasuyuki Yenni Ciawi Zaetuna, Hanifa Laila