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Learning Bats Handling for Oral Swab Sampling Azmi, Sevira Nur; Rustiati, Elly Lestari; Priyambodo, Priyambodo; Saswiyanti, Enny; Srihanto, Eko Agus; Pratiwi, Dian Neli; Master, Jani; Susandi, Waryoko
Jurnal Agrosci Vol 3 No 4 (2026): Vol 3 No 4 March 2026
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/agrosci.v3i4.1154

Abstract

Background. The only flying mammals, bats play an essential role in their natural habitat. Based on the type of food, bats can be divided into two groups: fruit-eating bats and insect-eating bats. Bats rank second-highest in species diversity, with 1,439 worldwide and 239 in Indonesia. Known as reservoirs of viruses, including coronaviruses. Bats are suspected to be related to COVID-19. Aims. Under the Research Innovation and Collaboration Program - Higher Education for Technology and Innovation Project (HETI) University of Lampung 2024-2025, and in collaboration with the Lampung Disease Investigation Centre, learning the procedures for handling bats properly so as not to physically harm bats in oral swab sampling and species recognition in Braja Harjosari, directly next to Way Kambas National Park was done. The life-trapping technique uses a mist net. Methods. Bat handling for taking an oral swab was carried out using the pinch grip method, holding both bat arms backwards with the thumb and middle finger, with the bat positioned facing upwards. Conclusion. The bat's mouth is then blown open, and a cotton swab is gently inserted into the bat's mouth. Afterwards, the bat is rested and given a drink/water and released into nature. Oral swab samples from 10 individual bats, fruit-eating bats, Cynopterus brachyotis (n = 8), Cynopterus horsfieldii (n = 1), and an insect-eating bat, Scotophilus kuhlii (n = 1).
RNA Isolation from Oral Swab Sample On Bats of Rural Area, Braja Harjosari, East Lampung Kurniasih, Vidyanti; Rustiati, Elly Lestari; Priyambodo, Priyambodo; Srihanto, Eko Agus; Saswiyanti, Enny; Pratiwi, Dian Neli
Jurnal Agrosci Vol 3 No 3 (2026): Vol 3 No 3 January 2026
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/agrosci.v3i3.1161

Abstract

Background. Bats are considered animals that carry infectious diseases, are known to be reservoirs of viruses, and are suspected of causing disease outbreaks. The World Health Organization stated that this disease outbreak is caused by Coronavirus Disease 2019 (COVID-19), which is caused by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2). Aims. Under the Research Innovation and Collaboration Program - Higher Education for Technology and Innovation Project (HETI) and in collaboration with the Lampung Disease Investigation Center, a preliminary study on EID detection on bats based on Predict Protocols was done to identify the presence of coronavirus on bats, including rural areas, Braja Harjosari, East Lampung. It is located next to Way Kambas National Park. Methods. Oral swab samples were taken from ten bats, 3 species: Cynopterus brachyotis and Cynopterus horsfieldi, and Scotophilus kuhlii. RNA extraction was conducted by Predict Protocols, which has four stages: lysis, binding, washing, and elution. Conclusion. Two samples showed thin-band luminescence. While in the quantity test, there are three samples of good concentration results between 1.8 and 2.0.
Genetic Research Trends in Caffeine Biosynthesis of Robusta Coffee (Coffea canephora) Permatasari, Nindy; Sari, Sismita; Nurmayanti, Sri; Fauziah, Lu'lu' Kholidah; Sari, Resti Puspa Kartika; Hardani, Maisuri; Aliyah, Siti Hamidatul; Priyambodo, Priyambodo
Jurnal Biologi Tropis Vol. 26 No. 2 (2026): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i2.11738

Abstract

Research on caffeine biosynthesis in Coffea canephora has expanded across multiple disciplines, yet its global genetic research trends remain insufficiently mapped. This study aimed to analyze the development and thematic structure of genetic research on caffeine biosynthesis in C. canephora using a bibliometric approach. Data were retrieved from the Scopus database through keyword-based sampling and filtered based on relevance to genetic and biosynthetic aspects. Bibliometric analyses were conducted to examine publication trends, authorship patterns, country contributions, and keyword co-occurrence networks. The results show a significant increase in publications since the early 2000s, with dominant contributions from the United States, Brazil, and China. Keyword mapping reveals four major research clusters, including plant metabolism, clinical and nutritional studies, experimental models, and genetic variability. However, studies explicitly focusing on the genetic regulation of caffeine biosynthesis, particularly involving N-methyltransferase genes, remain relatively limited compared to broader multidisciplinary themes.These findings indicate that caffeine research in C. canephora is highly interdisciplinary but still lacks a strong emphasis on genetic mechanisms, highlighting the need for more targeted molecular and genomic studies to support coffee improvement and functional trait development.
Co-Authors 'Aliyah, Siti Hamidatul Adelia, Ersa Imelda Adhiak, Annisa Nur Adhiak Afandi, Aril Afifi, Mohammad Hafizh Agus Srihanto, Eko Aliyah, Ni’matul Aliyah Apriani, Vivin Apriliasari, Messy Armando, M Zona Bintang Ashari, Minanti Mayda Aspita Laila Az Zahra, Flora Bintang Azmi, Sevira Nur Azzahra, Laila Salwa Azzaid, Fahrez Isnanta Baruno, Agustiawan Djoko Brumadyadisty, Garry Buhani Buhani Busman, Hendri Busman DEDI CANDRA Devi Krismuniarti, Elisabeth Djoko Baruno, Agustiawan Eko Agus Srihanto, Eko Agus Elly Lestari Rustiati Endah Setyaningrum, Endah Erdi Suroso Erika Diana Risanti Ernawiati, Eti Fahrezi, Aditya Farid, Muhammad Faiz Fathiyya, Naila Nur Fauziah, Lu'lu' Kholidah Febriansyah, Muhammad Fitrisyah, Asyifa Zahara Gina Dania Pratami, Gina Dania Heri Satria Ikhsanudin, Ahmad Ikhsanudin, Ahmad Ikhsanudin Imtitsal, Aulia Indah Ayu Lestari, Indah Ayu Jani Master, Jani John Hendri JOKO SUSANTO Junaidi Junaidi Koentjoro, Dani Djati Krismuniarti, Elisabeth Devi Kurniasih, Vidyanti Kusuma, Andriyani Wijaya Lestari Rustitati, Elly Lestari, Septi Wahyu Liza Angeliya Lumbanraja, Favorisen R Maharani, Annisa Lidya Maisuri Hardani, Maisuri Manik, Lastiarma Master, Jani Master Melya Riniarti Mohammad Kanedi, Mohammad Mutia, Aprila Nayla, Azza Dera Rahma Neli Pratiwi, Dian Nevrettia Christantyawati, Nevrettia Niken Satuti Nur Handayani Novianasari, Tika Nugraha, M. Aqwam Nuning Nurcahyani Nurmayanti, Sri Parabi, M. Iqbal Pawaka, Arrahmaan Syah Permatasari, Nindy Pratama, M. Idris Afta Pratama, Ngakan Putu Agung Dharma Pratama Pratiwi, Dian Neli Priyanatha, Dzulfikar Yusuf Priyanatha, Dzulfikar Yusuf Priyanatha Priyanto, Davit Agus Putri, Desma Ramadhina Qoyima, Allafia Raden Mohamad Herdian Bhakti Ramadan, Viki Ramadhan, Viki Rhamadaningtyas, Nabila Aulia Rinawati Rinawati Rochmah Agustrina Romanda, Fitka Royani, Vika Aulia Rusitati, Elly Lestari Rustiaati, Elly Lestari Sa'uddah, Lousanja D. Sabana, Amaliya Safitri, Anggi Sandra, Shifa Saputra, Rama Arsalta Bara Saputri, Nur Ayu Sari, Resti Puspa Kartika Saswiyanti, Enny Sa’uddah, Lousanja Dira Sidik, Mahfud Simatupang, Dwi Rani Asfarini Siringo-ringo, Regina Stephani Mesina Sismita Sari, Sismita Sitorus, Justin S Sitorus Slamet Riyadi Soegiharto, Yolande Cathleya Sufa, Siska Armawati Suhada Suhada, Suhada SUMADI SUMADI Susandi, Waryoko Susanto, Alvin Wiwiet Syarif Hidayat Amrullah Taufik, Nadya Syifa Thesalonika, Natasya Utoyo, Laji Vega, Cindy Ameliya Vika Aulia Royani Virnarenata, Elsa Wan Abbas Zakaria Warsono Warsono Winarno Winarno WR, Dilla Joedi Yani, Ani Andri Yanti Yulianti Yuliana Andriyani ‘Aliyah, Siti Hamidatul