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All Journal BALI MEDICAL JOURNAL (BMJ) Medicina journal of internal medicine INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES E-Jurnal Medika Udayana Majalah Ilmiah Fisioterapi Indonesia E-Jurnal Udayana Medica WMJ (Warmadewa Medical Journal) The Indonesian Biomedical Journal Oto Rhino Laryngologica Indonesiana ISM (Intisari Sains Medis) : Jurnal Kedokteran Bioscientia Medicina : Journal of Biomedicine and Translational Research INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Review of Primary Care Practice and Education (Kajian Praktik dan Pendidikan Layanan Primer) Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) International Journal of Nasopharyngeal Carcinoma PREPOTIF : Jurnal Kesehatan Masyarakat Bioscientia Medicina : Journal of Biomedicine and Translational Research Journal of Global Pharma Technology Eduvest - Journal of Universal Studies Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA) Physical Therapy Journal of Indonesia Biosaintifika: Journal of Biology & Biology Education
I Wayan Putu Sutirta Yasa
Bagian Patologi Klinik Fakultas Kedokteran Universitas Udayana
Aerospace Engineering Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Education Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences

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GAMBARAN UMUM HASIL PEMERIKSAAN DARAH LENGKAP PADA PASIEN TERINFEKSI VIRUS DENGUE PRIMER DAN SEKUNDER DI RSUP SANGLAH DENPASAR I Kadek Arya Candra; I Wayan Putu Sutirtayasa; Anak Agung Wiradewi Lestari
E-Jurnal Medika Udayana Vol 6 No 7 (2017): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

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Abstract

The diagnosis of primary or secondary Dengue virus infection is quite difficult because of the wide spectrum of symptoms and signs. Complete blood count testing may be the initial screening to determine diagnosis and therapy accurately. This study aimed to determine the general description of the results of a complete blood count includes the number of platelets, leukocytes, hematocrit, and hemoglobin in patients with primary and secondary Dengue virus infection. This was a descriptive cross-sectional study by taking data from medical record retrospectively with total sampling methode. Samples were patients with primary and secondary Dengue virus infection listed on Sanglah Denpasar Hospital during July 2013 - June 2014. The variables were analyzed by SPSS for Windows. The total sample were 202 peoples (46 primary-infected patients and 156 secondary-infected patients). The proportion of primary-infected patients and secondary-infected patients who has platelet count ?47.750 x 103/mL was 17.4% and 59.6%, the proportion of patients with leukocyte counts >3.965 x 103/mL was 30.4% and 55.8%, the proportion of patients with hematocrit >45.04% was 41.3% and 55.1%, and the proportion of patients with a hemoglobin level of >15.3 g/dL was 39.1% and 51.9%. It can be concluded that the majority of secondary-infected patients had lower platelet counts, but has the leukocyte count, hematocrit, and hemoglobin higher than the primary-infected patients. However, further research is needed to determine the relationship between the variables. Keywords: platelet, leukocyte, hematocrit, hemoglobin, primary and secondary Dengue virus infection.
APLASTIC ANEMIA Ni Made Dharma Laksmi; Sianny Herawati; Wayan Putu Sutirta Yasa
E-Jurnal Medika Udayana vol 2 no 7 (2013):e-jurnal medika udayana
Publisher : Universitas Udayana

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Abstract

Aplastic Anemia describes a disorder of the clinical syndrome is marked by a deficiency of red blood cells, neutrophils, monocytes and platelets in the absence of other forms of bone marrow damage. Aplastic anemia is classified as a rare disease in developed countries the incidence of 3-6 cases / 1 million inhabitants / year. The exact cause of someone suffering from aplastic anemia also can not be established with certainty, but there are several sources of potential risk factors. Prognosis or course of the disease varies widely aplastic anemia, but without treatment generally gives a poor prognosis
LABORATORY DIAGNOSIS OF HEPATITIS B Haneetha Yogarajah; AA Wiradewi Lestari; IWP Sutirta Yasa
Bali Journal of Medical and Health Sciences vol 1no1 2013
Publisher : Faculty of Medicine, Udayana University

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Abstract

Hepatitis B is a liver disease caused by a virus known as Hepatitis B virus (HBV) and is a major global health probelm. The liver is susceptible to a wide variety of adverse effects caused by an excess of alcohol, drugs, infections such as viral health department. Vaccination for children, adolescents and high-risk adults could also prevent hepatitis B. The prognosis is variable hepatitis, cancer and other metabolic disorders. Hepatitis B is the causative agent and belongs to the hepadnaviridae family of viruses. Transmission of HBV virus is through exposure to infected blood and body fluids. One of the important diagnostic criteria of Hepatitis B is by performing a thorough laboratory investigation.
IMMUNOPATHOGENESIS OF DENGUE SHOCK SYNDROME Karta Sawenda; I Wayan Putu Sutirta Yasa; DAP Rasmika Dewi; Bagus Komang Satriyasa
Bali Journal of Medical and Health Sciences vol 1no1 2013
Publisher : Faculty of Medicine, Udayana University

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Abstract

Dengue is the most important emerging tropical viral disease of humans in the world today. It is estimated that there are between 50 and 100 million cases of dengue fever (DF) and about 500.000 cases of dengue haemorrhagic fever (DHF) each year which require hospitalization. Persons of all ages can be infected and develop dengue fever. However, children younger than 15 years typically present with only a nonspecific self-limited febrile illness. Untreated dengue hemorrhagic fever mostly likely progresses to dengue shock syndrome which is defined as dengue fever with signs of circulatory failure. The prognosis depends on prevention or early recognition and treatment with case fatality rate is as high as 12% to 14% once shock has set in. Management of dengue fever requires rest, oral fluids to compensate for losses via diarrhoea or vomiting, antipyretics and analgesics. Patients who present with shock may require central venous pressure monitoring. An arterial line may be required in unstable patients for the assessment of blood gases, electrolytes, and coagulation profile to help identify patients needing ventilatory support.
THE CORRELATION OF TRANSAMINASES AND LIVER DISEASES Bastianus Alfian Juatmadja; I Wayan Putu Sutirta Yasa; DAP Rasmika dewi; Bagus Komang Satriyasa
Bali Journal of Medical and Health Sciences vol 1no1 2013
Publisher : Faculty of Medicine, Udayana University

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Abstract

The symptoms of liver diseases are very diverging, from the mild one till the severe one. Sometimes we may find that severe heart disorders but the symptoms are too less. We need some tools to make a good diagnosis. We can not only use a good anamnesis, but also have to use good physical examination and the other support test. Transaminase also called aminotransferase. This aminotransferase catalyzes the transfer of the amino group (?NH2) of an amino acid to a carbonyl compound. The liver contains specific transaminases for the transfer of an amino group from glutamic acid to ?-keto acids that correspond to most of the other amino acids. Other transaminases catalyze reactions in which an amino group is transferred from glutamic acid to other compounds. Transamination is one of the principal mechanisms for the formation of necessary amino acids in the metabolism of proteins. Transaminase as a sign to cell damage may divided into Serum Glutamic Oxalocetic Transaminase (SGOT), Serum Glutamic Pyruvic Transaminase (SGPT), and Lactic Dehydrogenase (LDH). Gamma GT and alkali fosfatase correlate with cholestasis. Cholinestrase correlate with liver synthesis capacity.
Role of HIF-1, Siah-1 and SKN-1 in Inducing Adiposity for Caenorhabditis elegans under Hypoxic Conditions I Gede Widhiantara; Anak Agung Ayu Putri Permatasari; I Wayan Rosiana; I Wayan Putu Sutirtayasa; Ferbian Milas Siswanto
The Indonesian Biomedical Journal Vol 12, No 1 (2020)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v12i1.1007

Abstract

BACKGROUND: Hypoxia has been shown to be able to induce adiposity. However, the mechanism and factors involved in this effect still remains unclear. Hence, we sought to investigate the role of oxygensensitive factors regarding hypoxia-induced adiposity in nematode Caenorhabditis elegans.METHODS: The C. elegans were grown on nematode growth medium (NGM) agar plates seeded with Escherichia coli OP50 at 20°C. The ratio of width/body length was measured using the morphometry analysis. Fat accumulation was examined using Sudan Black methods. Protein levels of sterol binding protein (SBP)-1 were assessed by immunoblotting. Lifespan assay was performed at 20°C and was monitored every two days.RESULTS: The results showed that of all mutant used, only hif-1 mutant which did not experience an increase in the ratio of width/body length (p>0.05) and fat accumulation (p>0.05), indicating that hypoxia-inducible factors (HIF)-1 plays an important role in the pathogenesis of hypoxia-induced adiposity. Both siah-1 and skn-1 mutants experienced SBP-1 protein elevation (p<0.05), and increased fat-6 mRNA expression (p<0.05) which were not experienced by a hif-1 mutant (p>0.05) further supporting that HIF-1 acts as an upstream regulator fromSBP-1.CONCLUSION: In general, the results of this study provide evidences of the involvement of the transcription factor HIF-1 in inducing adiposity under the hypoxic conditions. However, we did not find the involvement of seven in absentia homolog-1 (Siah-1) and skinhead-1 (SKN-1).KEYWORDS: hypoxia, adiposity, fat, HIF-1, Siah-1, SKN-1, C. elegans
SARI CenTeLLA ASIATICA ASLI BALI MENINGKATKAN SEKRESI TuMouR neCRoSIS FACToR ALPHA (TNF-a) PADA MENCIT YANG DIINFEKSIKAN SALMoneLLA TYPHI I Nyoman Wande; Sianny Herawati; Ida Ayu Alit Widhiartini; I Wayan Putu Sutirta Yasa; Tjokorda Gede Oka; Ni Made Linawati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 3 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i3.477

Abstract

Tumour necrosis factor alpha (TNF-α) is a cytokine produced by macrophages and other mononuclear cells, is a good antibacterial agent against Salmonella spp, especially Salmonella typhi. Centella asiatica is an alternative drug that is expected as an immunostimulant in patients with typhoid fever. Comparing the effectiveness of Centella asiatica extract the original Bali as an immunostimulant and without stimulants in mice infected Salmonella typhi in terms of TNF-α secretion. This study is an experimental study with a post test only with control group design. A total of 20 mice were divided into 4 groups. The first and second groups each given Centella asiatica extract 75 mg/20 g bw (0.5 cc) and without a given extract for 4 weeks. Both groups were inoculated orally Salmonella typhi 106 per mL of bacteria in the second week. The third and fourth groups were given thiamphenicol with Centella asiatica extract 75 mg/20 g bw (0.5 cc) and thiamphenicol without any extract for 4 weeks respectively. Both groups were inoculated orally Salmonella typhi 106 per mL of bacteria in the first day. All groups terminated on fourth week and examination levels of TNF-α by ELISA and gall culture. The mean levels of TNF-α in groups (1–4) is 86.10±2.67 pg/mL, 32.81±11.33 pg/mL, 35.87±3.90 pg/mL and 19.21±2.19 pg/mL respectively. Based on the examination of the gall cultures this study showed positive results in the first and second groups, while a negative result on the third and fourth groups. Based on the One way ANOVA analysis on levels of TNF-α, there are significant differences between the first group with the second group (p<0.05), and between the third and fourth groups also found significant differences (p<0.05) increased levels of TNF-α in mice with Salmonella typhi infection given Centella asiatica extract.
ACETOSAL, BUAH MENGKUDU (MORINDA CITRIFOLIA L.) DAN WAKTU PERDARAHAN I Wayan Putu Sutirta Yasa; Ketut Widyani Astuti; I Gusti Made Aman
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 2 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i2.1007

Abstract

Research regardless the effect of noni fruit for increasing bleeding time have already been carried out widely. The similar activity ofnoni fruit extract and acetosal can be concerned that the fruit extract has a potential activity for prolonged bleeding time. This study aimsto know the present of prolonged of bleeding time as a results of intake of combination of noni fruit extract with acetosal on mice. Thisresearch was carried out at Unit Binatang Percobaan, Departemen Farmakologi Universitas Udayana. This is an experimental study withpre and post-test control group design. Subject was compromised of 3 groups of mice and each group contain of 7 mice. The first groupwas treated with a dose of 40 mg/kg bw acetosal, the second group treated with a dose of 100 mg/kg bw ethanol noni fruit extract, andthe third group treated with combination of 40 mg/kg bw acetosal and 100 mg/kg bw ethanol extract of noni fruit. All groups werefed once per day for a week. Bleeding time was determined on the basis of tail bleeding method. This study results that the first groupexperience bleeding time increased from 61.42±9.43 second to 160.71±19.77 second. Increase bleeding time of the second group is from59.14±7.12 to 138.14±59.91 second. For the third group, the bleeding time increases from 65.00±7.91 to 213.00±20.92 second.One Way ANOVA analysis indicates that there is a significant different among these three groups after treatment p = 0.006 (p<0.05).Bleeding time of the third group which was treated with combination of noni fruit and acetosal results in the highest increase compareto the other two groups. In conclusions, combination of noni fruit and acetosal treatment results in increase of bleeding time on mice.
KADAR IL-6 PLASMA PASIEN DIABETES MELITUS DENGAN DAN TANPA PENGIDAP RETINOPATI DIABETIKA (The Level of Interleukin-6 Plasma in Diabetes Mellitus Patients With and Without Diabetic Retinopathy) I Wayan Putu Sutirta Yasa; I Nyoman Wande; Ni Ketut Niti Susila; Putu Budhiastra; Cokorda Istri Dewiyani Pemayun; Sianny Herawati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 1 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i1.1249

Abstract

Diabetic retinopathy is a complication of diabetes mellitus in the form of microvascular (aneurysma) which can cause blindness.Interleukin-6 is a pro - inflammatory cytokine produced by monocyte macrophage in the early inflammation. Chronic inflammationplays the role on artherosclerosis event, because it is directly correlated with all phases of it and cardiovascular disease as well. Theaim of this study is to know the difference in plasma IL-6 levels among patients suffering from diabetes mellitus together with diabeticretinopathy and without diabetic retinopathy. This study is an observational analytical research with cross sectional design. The sampleswere taken randomly as many as 16 patients, divided in each group suffering from or without retinopathy. The diabetic mellitus patientswere established through their related history and were examined randomly for blood glucose and their fundus or retinal abnormality.The level of IL-6 was examined by ELISA method with double antibody sandwich streptavidin - biotin. Based on this study it was foundthat the mean plasma levels of interleukin 6 (IL-6) of the diabetic mellitus patients with retinopathy was 1.165 ± 0.296 pg/mL andthe mean plasma levels of IL-6 with diabetes mellitus without retinopathy was 0.879 ± 0.230 pg/mL. Based on the normality test withShapiro-Wilk the data showed a normal distribution (p>0.05), while the Levene’s homogenity test obtained homogeneous with p= 0.369(p>0,05). The statistical analysis with two independent samples t-test showed that there were significant differences between the groupsof patients who suffered diabetes mellitus with retinopathy and without retinopathy (p=0.005). It can be concluded so far that the meanlevels of interleukin-6 (IL-6) plasma in diabetic mellitus patients with retinopathy is higher compared to patients with the same diseasebut without retinopathy.
INFEKSI VIRUS RABIES DI ANAK-ANAK A.A.N. Subawa; DGD. Dharma Santhi; A.A Raka Sudewi; IWP. Sutirta Yasa
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 3 (2011)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (566.285 KB) | DOI: 10.24293/ijcpml.v17i3.1093

Abstract

Rabies has been known in ancient Egypt and China since the fifth century. The disease is caused by the Rhabdovirus of the genusLyssavirus spread from animals or bats to humans by saliva. A five-year-old kid was admitted to Sanglah General Hospital on February19. 2010 with fever complaints, before admitted to the hospital, since the morning he always stuck out his tongue, bit his lips, drinkinga little of water and hard to swallow. From the anamnesis, it is known that the patient has a history of bitten by a dog on February 4.2010 at the lower eyelid and had received wound treatment and VAR, after a while having the treatment the patient eventually died onFebruary 21. 2010. According to the post-mortem sample of the LCS it is showed a positive PCR result of rabies virus infection.
Co-Authors Achmad Chusnu Romdhoni Adhitya, Putu Gde Surya Agung Nova Mahendra Agung Wiwiek Indrayani Airin Que Ali Djamhuri Amaral, Meriana Barreto Anak Agung Gede Sudewa Djelantik Anak Agung Ngurah Subawa Anak Agung Wiradewi Lestari Anak Agung Wiradewi Lestari, Anak Agung Anom Suardika Astini, Dewa Ayu Agung Alit Suka Bagus Komang Satriyasa Bastianus Alfian Juatmadja Benny Supono Calvin Jonathan Cokorda Agung Wahyu Purnamasidhi Cokorda Istri Dewiyani Pemayun D.P.G. Jananuraga Maharddhika Desak Gde Diah Dharma Santhi Dewa Ayu Putu Rasmika Dewi Dewi, Ni Kadek Ari Kusuma Dharayani, Luh Anandita Dharma Santhi, Desak Gde Diah Dina Sophia Margina Divasta, I G Mahapraja Fatqur Rochman Feliciano Pinto, Feliciano Ferbian Milas Siswanto Gde Ary Putra Kamajaya Gede Agus Eka Tirta Putra Gede Agus Suwiryawan Gusti Ngurah Sutapa Hamsu Kadriyan Haneetha Yogarajah I A Putri Wirawati I Dewa Made Sukrama I Gde Raka Widiana I Gde Suranaya Pandit I Gede Juliarta I Gede Widhiantara I Gst Agung Dwi Mahasurya I Gusti Agung Dyah Ambarawati I Gusti Agung Ngurah Radhitya Wijaya Radhitya Wijaya I Gusti Kamasan Arijana I Gusti Made Aman I Kadek Arya Candra I Kadek Septiawan I Ketut Agus Somia I Ketut Suastika I Ketut Suwiyoga I Made Ady Wirawan I Made Bagus Cahya Wibawa I Made Bakta I Made Dwikayana I Made Jawi I Made Oka Adi Parwata I Made Sudarmaja I Made Tomik Nurya Wardana I NYOMAN MANTIK ASTAWA I Nyoman Wande I Nyoman Wande I W. Sudarsa I Wayan Gede Artawan Eka Putra I Wayan Rosiana I Wayan Sumardika I Wayan Wita I. B. T. Wibawa Manuaba, I. B. T. Wibawa I. B. Tjakra Wibawa Manuaba I. K. Widiana, I. K. I.A.A. Widhiartini I.B.GDE ANANTA MAHESVARA IBN Dwipayana Manuaba Ida Ayu Kemala Wasita Manuaba Ida Bagus Made Suryatika Ida Bagus Ngurah Ida Bagus Putra Manuaba Ida Bagus Verry Kusumaningrat Ida Bagus Wayan Kardika Ida Kurniawati, Ida IGA Ayu Ratih Pradnyadewi Ignatius Ferdi Yuatmadja Indah Pramita Indradewa, Rhian Indrayani, Pande Kadek Putri Rahayu Intan Astariani Kamayoga, I Dewa Gede Alit Karta Sawenda Ketut Siki Kawiyana Ketut Suega Ketut Suryana Ketut Tuti Parwati Merati Ketut Widyani Astuti Lesmana, Cokorda Agung Bagus Jaya Lesmana, I Wayan Lolik Luh Putu Sukma Diyanti Made Ayu Widyaningsih Made Dyah Khrisnadewi MADE RATNA SARASWATI . Melania Antonia Barreto Cerqueira Michael Christian Widjaja Milaviwanda, Luh Komang Ayu N. K. Niti Susila, N. K. Naw, Sin War Ni Gusti Ayu Putu Lestari Santika Dewi Ni Kadek Mulyantari Ni Kadek Nita Utami Ni Ketut Susilawati Ni Ketut Susilawati Ni Luh Candra Mas Ayuni Ni Luh Wayan Pani Ambarasari Ni Made Dharma Laksmi Ni Made Linawati Ni Nyoman Mahartini Ni Nyoman Mahartini Ni Nyoman Mahartini Ni Putu Oktaviani Rinika Pranitasari Ni Putu Widya Nandasari Ni Wayan Ari Anindita Sari Ocktaviana Saputri, Legis Pande Ayu Naya Kasih Permatananda Pande Komang Gede Bayu Wikrama Permatasari, Anak Agung Ayu Putri Poniman, S. Prabasari, Pande Visca Gayatri pradnya wibawa, komang budhi Pratiwi, Cokorda Agung Pujawan, I Made Naris Purnamawati, Susy Puspa Negara, Anak Agung Gede Angga Putu Angga Wiradana Putu Astawa Putu Budhiastra Putu Diani Wirayanti Raka-Sudewi A. A. Rudi Wisaksana S. Herawati, S. Sagung Novita Widyaningrat Saisnu Supta, I Wayan Santosa, I Gusti Ngurah Putra Eka Sianny Herawati Sri Maliawan Sri Wahyuni Susy Purnawati Swari, Kadek Gyna Yadnya Thaha .. Tirtha Yasa, I Nyoman Wawan Tjokorda Gde Agung Suwardewa Tjokorda Gede Oka Tri Pramartha, I Nyoman Valari, Putu Kavita Krisnina Wayan Suardana Widiyanti, I Gusti Ayu Wijaya Kusuma Yayang Christian Yenny Kandarini