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<![CDATA[INFEKSI VIRUS RABIES DI ANAK-ANAK ]]>
<![CDATA[A.A.N. Subawa]]>;
<![CDATA[DGD. Dharma Santhi]]>;
<![CDATA[A.A Raka Sudewi]]>;
<![CDATA[IWP. Sutirta Yasa]]>
<![CDATA[ INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 3 (2011) ]]>
Publisher : <![CDATA[Indonesian Association of Clinical Pathologist and Medical laboratory ]]>
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DOI: 10.24293/ijcpml.v17i3.1093
<![CDATA[Rabies has been known in ancient Egypt and China since the fifth century. The disease is caused by the Rhabdovirus of the genusLyssavirus spread from animals or bats to humans by saliva. A five-year-old kid was admitted to Sanglah General Hospital on February19. 2010 with fever complaints, before admitted to the hospital, since the morning he always stuck out his tongue, bit his lips, drinkinga little of water and hard to swallow. From the anamnesis, it is known that the patient has a history of bitten by a dog on February 4.2010 at the lower eyelid and had received wound treatment and VAR, after a while having the treatment the patient eventually died onFebruary 21. 2010. According to the post-mortem sample of the LCS it is showed a positive PCR result of rabies virus infection.]]>
<![CDATA[NEOPTERIN DAN PEROKSIDA SERUM SEBAGAI PETANDA MAKROFAG TERAKTIVASI PADA TUBERKULOSIS PARU AKTIF DAN INDIVIDU BERKEBAHAYAAN TINGGI (Serum Neopterin and Peroxide As Marker of Activated Macrophages on Active Pulmonary Tuberculosis and Individuals At High Risk) ]]>
<![CDATA[I Nyoman Wande]]>;
<![CDATA[Ni Made Linawati]]>;
<![CDATA[I Made Bagiada]]>;
<![CDATA[IWP. Sutirta Yasa]]>;
<![CDATA[AAN. Subawa]]>
<![CDATA[ INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 1 (2015) ]]>
Publisher : <![CDATA[Indonesian Association of Clinical Pathologist and Medical laboratory ]]>
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DOI: 10.24293/ijcpml.v22i1.1227
<![CDATA[Failure of macrophages to phagocytize Mycobacterium tuberculosis causes the release of hydrogen peroxide/peroxide (H2O2) bythe activated macrophages. Neopterin is one of the most important markers in the activated macrophages. Neopterin is a pteridinederivative produced by the activated macrophages through the stimulation of interferon gamma. Increased levels of Neopterin has beenreported in lung tuberculosis. Activation of macrophages is essential to the development of tuberculosis infection that can lead to activepulmonary tuberculosis or latent tuberculosis, in this case is a high-risk for healthy individuals. To determine the differences in serumlevels of Neopterin and H2O2 between patients with active pulmonary tuberculosis and healthy individuals at high risk of pulmonarytuberculosis. A total of 15 patients with active pulmonary tuberculosis and 15 healthy individuals at high risk examination serumNeopterin levels and peroxide (H2O2). Active pulmonary tuberculosis patients when the results of sputum smear examination chest x-raysis obtained positive results. Healthy individuals at high risk when the results of smear examination and chest x-rays is negative. Thelevel of Neopterin was examined using a double antibody sandwich immunoassay with Human neopterin (NEOP) ELISA Kit ® BioassayTechnology Laboratory. The level of peroxide was examined using quantitative colorimetric peroxidedetermination with QuantiChromTMPeroxide Assay Kit (DIOX-250)®. Neopterin serum levels between patients with active pulmonary tuberculosis and healthy individualsat high-risk were analysed by independent samples t-test. H2O2 serum levels between patients with active pulmonary tuberculosis andhealthy individuals at high-risk were analysed with Mann Whitney Test. The confidence level is p <0.05. The mean Neopterin levelsin patients with active pulmonary tuberculosis was 5.17±4.64 nmol/L, the mean Neopterin levels in group of healthy individuals athigh risk was 3.97±1.79 nmol/L. Statistical analysis by the independent samples t-test found no significant differences between groupsin Neopterin serum levels of patients with active pulmonary tuberculosis and healthy individuals at high risk (p=0.357). The meanserum levels of H2O2 of group of patients with active pulmonary tuberculosis was 26.38±3.00 μM, the mean levels of H2O2of group ofhealthy individuals at high risk of 20.69±4.46 μM. Statistical analysis with non-parametric Mann-Whitney Test was found significantdifference in the peroxide (H2O2) levels between groups of patients with active pulmonary tuberculosis and group of healthy individualat high-risk (p=0.000). The levels of Neopterin in patients with active pulmonary tuberculosis was not significantly higher comparedto the healthy individuals of high risk. Levels of peroxide (H2O2) serum in patients with active pulmonary tuberculosis was significantlyhigher compared to the group of healthy individuals at high risk. This shows that there is increased activity of macrophages in patientswith active pulmonary tuberculosis, but not effective in eliminating of Mycobacterium tuberculosis.]]>
<![CDATA[SUHU PENYIMPANAN KREATININ DAN ASAM URAT DALAM AIR KEMIH SELAMA 24 JAM ]]>
<![CDATA[AAN. Subawa]]>;
<![CDATA[Sianny Herawati]]>;
<![CDATA[I Nyoman Wande]]>;
<![CDATA[I Wayan Putu Sutirta Yasa]]>;
<![CDATA[Tjokorda Gede Oka]]>
<![CDATA[ INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 2 (2015) ]]>
Publisher : <![CDATA[Indonesian Association of Clinical Pathologist and Medical laboratory ]]>
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DOI: 10.24293/ijcpml.v21i2.1107
<![CDATA[Creatinine and uric acid is a product that excreted in the urine by normal kidney functions. The examination of creatinine and uricacid in urine is done on 24-hour urine collection. During the storage of the urine, it is recommended to be stored in a refrigerator withthe grade temperatures ranging from 2–8°C and is not recommended to use any preservative for the examination of creatinine anduric acid in urine. To know the comparation of creatinine and uric acid concentrations in urine between the urine tested immediatelyafter the collection with urine that was stored at a temperature 2–8°C and those at room temperature for 24 hours. A total of 45 urinesamples from outpatient clinic that came to the laboratory, were collected in particular urine vacutainer. Each urine sample is divided intothree tubes. The first tube (P1) examined concentrations of creatinine and uric acid immediately after collection, was considered as thebaseline value. The second tube (P2) stored at 2–8°C and the third tube (P3) is stored at room temperature for 24 hours, then followedby the examination of creatinine and uric acid concentrations. The examination of creatinine in urine was using reagent CREP2 RocheDiagnostic and uric acid in urine was using reagent UA2 Roche diagnostics by Cobas Integra ® 400 plus ® instrument. The mean ofcreatinine in urine concentrations which immediately examined (P1) is (125.10±74.85 mg/dL), concentrations after storage at 2−8°C(P2) and at room temperature (P3) were (123.42±73.80 mg/dL) and (124.09±73.95 mg/dL) respectively. Based on the analysis ofone-way ANOVA, there were no significant differences between the concentrations of creatinine in urine immediately checked which werestored at 2–8°C and at room temperature (P>0.05). The mean of uric acid in urine concentrations which immediately examined (P1) is(52.61±35.48 mg/dL), where as after storage at 2–8°C (P2) and room temperature (P3) were (45.11±31.62 mg/dL) and (46.38±28.91mg/dL) respectively. Based on the analysis of one-way ANOVA, there were no significant differences between the concentrations of uricacid in urine immediately checked by those stored at 2–8°C and at room temperature (P>0.05). Based on this study, it can be concludedthat there were no effect of storage temperature on the concentrations of creatinine and uric acid in urine within 24 hours.]]>
<![CDATA[UMBI UBI JALAR UNGU BALI (Ipomoea batatas) DI TRANSAMINASE SERUM, MALONDIALDEHIDE HEPAR DAN ALKOHOL KRONIS ]]>
<![CDATA[I Wayan Putu Sutirta-Yasa]]>;
<![CDATA[I Made Jawi]]>;
<![CDATA[Ida Bagus Ngurah]]>;
<![CDATA[Anak Agung Ngurah Subawa]]>
<![CDATA[ INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 3 (2011) ]]>
Publisher : <![CDATA[Indonesian Association of Clinical Pathologist and Medical laboratory ]]>
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DOI: 10.24293/ijcpml.v17i3.1170
<![CDATA[Oxidative stress as a consequence of chronic alcoholic intake causes lesions of liver tissue. The objective of this study was to identifythe antioxidant effect of ethanol extract derived from Balinese purple sweet potato on the liver tissue in mice after chronic consumptionof alcohol. Subjects of this study were 32 adults male Swiss mice (12–14 weeks) divided into 4 groups with control group post-testonly design. Post-treatment of blood and liver samples were collected from each group of 8 mice. The MDA liver was quantified withthiobarbituric acid reactive substances (TBARS) method. Samples were collected from control group of 8 mice without sweet potatoextract and alcohol, 8 mice with 1 mg extract of Balinese purple sweet potato, 8 mice with 0.8 gram alcohol, and 8 mice with combineof alcohol and extract of Balinese purple sweet potato, each treatment was carried out every day for 4 weeks. The results showed asignificant increase of serum SGOT, SGPT and liver MDA level, after chronic consumption of alcohol (p = 0.00). The decrease of serumSGOT, SGPT and liver level MDA was significant in the group with extract of Balinese purple sweet potato (p = 0.00). The researchersconcluded that the extract of Balinese purple sweet potato had an antioxidant effect in mice which consumed alcohol chronically. Basedon this study it is concluded that extract of Balinese purple sweet potato has a protective effect on the liver cell in mice which consumedalcohol chronically.]]>
<![CDATA[C-X-C RECEPTOR 4 {CXCR4} METASTASIS KANKER PAYUDARA ]]>
<![CDATA[I Wayan Sudarsa]]>;
<![CDATA[I Wayan Putu Sutirta Yasa]]>
<![CDATA[ INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 19, No 2 (2013) ]]>
Publisher : <![CDATA[Indonesian Association of Clinical Pathologist and Medical laboratory ]]>
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DOI: 10.24293/ijcpml.v19i2.1068
<![CDATA[The chemokine receptors CXCR4 (chemokine C-X-C motif receptor 4) and its ligand (stromal derived factor-1/SDF-1 or chemokinemotif ligand 12/CXCL12) play an important role in cancer invasion and metastasis. The spread of breast cancer follows a nonrandommetastatic pattern typically involving spread of tumor to regional lymph nodes, lung, liver, and/or bone marrow. The ligand for CXCR4,SDF-1/CXCL12, is highly expressed by stromal fibroblasts within these tissues. The chemokine receptors CXCR4 is structurally related tochemokine receptor belonging to the superfamily of the seven transmembrane G-protein coupled receptors. In contrast to normal breasttissue, breast cancer cells typically express high levels of functional CXCR4 receptors that can direct chemotaxis and invasive responses.Expression of SDF-1/CXCL-12 in turn, promotes the progression of breast cancer by directly enhancing tumor-cell growth and by recruitingendothelial progenitor cells that are required for tumor angiogenesis. High-level expression of CXCR4 on neoplastic cells is associated withrelatively poor overall survival and bad prognosis in patients with breast cancer. The promising results in the preclinical tumor modelsindicate that CXCR4 antagonists may have to reduce the spread of cancer that is called anti tumor activity in patients with breast cancer.The chemokine receptors CXCR4 antagonists, although initially developed for treatment of acquired immunodeficiency diseases syndrome(AIDS), actually may become effective agents as a molecular targeted therapy for breast cancer.]]>
<![CDATA[Purple Sweet Potato Reduces Malondialdehyde and TNF-a, Increases p53, and Protects Histopathological Appearance in Formaldehyde-induced Nasopharyngeal Carcinoma Rats ]]>
<![CDATA[Ni Ketut Susilawati]]>;
<![CDATA[I Wayan Putu Sutirta Yasa]]>;
<![CDATA[Wayan Suardana]]>;
<![CDATA[Sri Maliawan]]>;
<![CDATA[I Made Jawi]]>;
<![CDATA[Achmad Chusnu Romdhoni]]>;
<![CDATA[Hamsu Kadriyan]]>;
<![CDATA[I Wayan Gede Artawan Eka Putra]]>;
<![CDATA[I Gusti Kamasan Nyoman Arijana]]>
<![CDATA[ The Indonesian Biomedical Journal Vol 14, No 2 (2022) ]]>
Publisher : <![CDATA[The Prodia Education and Research Institute (PERI) ]]>
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DOI: 10.18585/inabj.v14i2.1906
<![CDATA[BACKGROUND:Purple sweet potato tuber ethanol extract (PSPTEE) has been known to have benefits in various disease, including to prevent nasopharyngeal carcinoma (NPC). However, there has been no research on PSPTEE in preventing the proliferation of nasopharyngeal mucosal epithelial cells induced by formaldehyde through the oxidative stress mechanisms and inflammatory process. Studies have proven the effect of PSP on various cancer cells, but the carcinogenesis process of the nasopharyngeal mucosal epithelium is still limited. Hence, this study aimed to demonstrate the mechanism for preventing histopathology changes with PSPTEE due to formaldehyde exposure.METHODS: Thirty-two formaldehyde-induced Wistar rats were treated with or without 1g/kgBW/day PSPTEE for 16 weeks. Malondialdehyde (MDA) level were examined with spectrophotometry method, while tumor necrosis factor (TNF)-α and p53 with enzyme linked immunosorbent assay (ELISA) method. The histopathology appearance of the nasopharyngeal epithelium was observed with hematoxylin-eosin (HE) staining.RESULTS: MDA and TNF-α levels in control and treatment group were 22.89±2.84 μM; 9.83±0.89 μM and 84.18±11.58 ng/L; 73.92±10.59 ng/L, respectively, and they showed significant difference. Meanwhile, the level of p53 showed no significant difference. Histopathology appearance showed a significant difference. Path analysis for MDA, TNF-α and p53 levels contributed 28.7% to histopathology appearance. MDA have an effect on significant direct effect on TNF-α. TNF-α has a significant direct effect to histopathological appearance. Indirect influence MDA on histopatological appearance were smaller than the direct effect. TNF-α has the greatest influence on histopatological appearance.CONCLUSION: Formaldehyde induces the histopathology appearance in nasopharyngeal epithelium. This study also demonstrates the mechanism to prevent the nasopharynx epithelial histopathology appearance by administration of PSPTEE.KEYWORDS: PSP, MDA, TNF-α, p53, nasopharynx epithelial]]>
<![CDATA[THE RELATIONSHIP BETWEEN THE STAGE OF NASOPHARYNGEAL CARCINOMA WITH ANEMIA AND NUTRITIONAL STATUS IN WEST NUSA TENGGARA GENERAL HOSPITAL ]]>
<![CDATA[Ni Ketut Susilawati]]>;
<![CDATA[Hamsu Kadriyan]]>;
<![CDATA[Wayan Putu Sutirtayasa]]>
<![CDATA[ INTERNATIONAL JOURNAL OF NASOPHARYNGEAL CARCINOMA Vol. 2 No. 01 (2020): International Journal of Nasopharyngeal Carcinoma ]]>
Publisher : <![CDATA[TALENTA PUBLISHER ]]>
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DOI: 10.32734/ijnpc.v2i01.3579
<![CDATA[Introduction: Nasopharyngeal carcinoma is the most common malignant tumor in the ENT-HNS were the highest of all malignant tumors of the head and neck. Malnutrition and cachexia is a common problem in cancer patients and impact on quality of life and survival. Anemia is a major problem in patients with nasopharyngeal cancer receiving chemotherapy or radiotherapy. Objective: Determine the relationship of nasopharyngeal carcinoma stage with anemia and nutritional status. Material and methods: This study used cross sectional analytic method. Data were obtained from medical records of patients with nasopharyngeal carcinoma who were treated in province hospital West Nusa Tenggara period January 2018 to December 2018. Data collected include the identity, diagnosis, hemoglobin value, weight and height. Having analyzed the data collected are looking for is there a relationship between stage nasopharyngeal carcinoma with anemia and nutritional status. Results: In this study, Shapiro-Wilk normality test. Anova is then performed to analyze the relationship stage nasopharyngeal carcinoma with anemia status obtained 95% CI, p value 0.847, there is no difference between stage nasopharyngeal carcinoma with anemia. Kruskal Wallis test to analyze the relationship with BMI (body mass index) with stage nasopharyngeal carcinoma obtained 95% CI, p value 0.565. There is no difference in BMI with stage nasopharyngeal carcinoma. Conclusion: There is a tendency to lower Hb in patients with nasopharyngeal cancer further stage although there is no significant difference between each stage. The same thing was found in BMI, BMI decreased tendency in patients with advanced nasopharyngeal cancer although there was no statistically significant difference.]]>
<![CDATA[Prevalensi anemia pada ibu hamil di Puskesmas IV Denpasar Selatan Tahun 2015 ]]>
<![CDATA[Yayang Christian]]>;
<![CDATA[I Wayan Putu Sutirta Yasa]]>
<![CDATA[ Intisari Sains Medis Vol. 10 No. 2 (2019): (Available online: 1 August 2019) ]]>
Publisher : <![CDATA[DiscoverSys Inc. ]]>
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DOI: 10.15562/ism.v10i2.277
<![CDATA[Introduction: The prevalence of pregnant women with anemia is quite high in Indonesia up to 63.5%. Though anemia can cause various complications in the fetus and in pregnant women themselves. There is still no data supporting the prevalence of anemia in pregnant women in Bali Province. The purpose of this study was to find the prevalence of anemia in pregnant women in Bali Province, especially the city of Denpasar and complete the data library regarding anemia in preganancy in Indonesia.Method: The research used cross-sectional design, and secondary data collection from medical record of Primary Health Care IV South Denpasar year 2015.Result: Anemia pregnant women amounted to 166 of total 521 pregnant women with 31.9% prevalence, with an average of 43 cases of pregnant women with anemia every month.Conclusion: From the data obtained found the prevalence of pregnant women who suffer from anemia is still relatively high (31.9%).]]>
<![CDATA[Karakteristik penderita suspect demam berdarah dengue berdasarkan penurunan trombosit di Instalasi Rawat Inap Puskesmas Tegalalang I tahun 2014-1015 ]]>
<![CDATA[Pande Komang Gede Bayu Wikrama]]>;
<![CDATA[I Wayan Putu Sutirta Yasa]]>
<![CDATA[ Intisari Sains Medis Vol. 10 No. 2 (2019): (Available online: 1 August 2019) ]]>
Publisher : <![CDATA[DiscoverSys Inc. ]]>
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DOI: 10.15562/ism.v10i2.290
<![CDATA[Introduction: Dengue hemorrhagic fever are found in sub-tropic area and tropical. In the last 50 years, the incident has increased 30 times in the world. World Health Organitation with more than 70 % of the population are at risk of dengue lives in Asia. In the year 2007, Indonesia is a country of dengue hemorrhagic fever cases in Southeast Asia. Tegalalang is one of the district in Gianyar, which was having an increase in dengue hemorrhagic fever cases. This study aims to determine the characteristics of patients with suspected dengue fever based on a decrease in platelets in Inpatient Unit/Installation of the Puskesmas (Public Health Centre) Tegalalang I in 2014-2015.Methods: Study design using cross sectional model with secondary data from Puskesmas (Public Health Centre) Tegalalang I started in January 2014 to June 2015. In the medical records of observable characteristics by sex, age, occupation, and decreased thrombocytes count began from the day third, fourth, and fifth of the fever.Results: From 92 samples, 62% of men and 38% of women. The most samples between 15-24 years old and students are the most. Average thrombocytes on patients who began to be hospitalized on third day of the fever is 142.76x103/ml. Average thrombocytes on patients who began to be hospitalized on four days of the fever is 123.22x103/ml and average thrombocytes on patients who began to be hospitalized on fifth day of the fever is 88.67x103/ml.Conclusion: It can be concluded that the number of thrombocytes decrease in patients with suspected dengue hemorrhagic fever can occurs on in the fever on day third, fourth and fifth.]]>
<![CDATA[Deteksi gen Gtf-B Streptococcus mutans dalam plak dengan gigi karies pada siswa di SD N 29 Dangin Puri ]]>
<![CDATA[I Gusti Agung Dyah Ambarawati]]>;
<![CDATA[I Dewa Made Sukrama]]>;
<![CDATA[I Wayan Putu Sutirta Yasa]]>
<![CDATA[ Intisari Sains Medis Vol. 11 No. 3 (2020): (Available online: 1 December 2020) ]]>
Publisher : <![CDATA[DiscoverSys Inc. ]]>
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DOI: 10.15562/ism.v11i3.337
<![CDATA[Background: Bacteria situated in the formation of dental plaque as a leading cause of caries is Streptococcus mutans. Streptococcus mutans use glycosyltransferase enzymes to convert saccharose saliva into an extracellular polysaccharide (PSE) through glycosylation process. One of the virulence factors of Streptococcus mutans is the gtf-B gene.Aim: This study aims to detect gtf-B gene in plaque with dental caries on students of SD Negeri 29 Dangin Puri.Method: The design of the study was descriptive observational research involved 51 carries children as a sample in SD Negeri 29, Dangin Puri. Bacterial culture was applied to detect colonies of Streptococcus. Additional gram staining and catalase test were also conducted to distinguish Streptococcus against Staphylococcus. After it revealed negative catalase test, PCR was continued optimally about 517 bp in size and 585 bp gtf B gene in size.Result and Conclusion: Streptococcus mutans are as many as 19 samples from 51 samples (37.25%). Three samples from 19 isolates of Streptococcus mutans were detected by gtf-B gen (16%). Bakteri yang berperan penting dalam pembentukan plak gigi sebagai penyebab karies adalah Streptococcus mutans. Streptococcus mutans memiliki enzim glikosiltransferase yang dapat mengubah sakarosa saliva menjadi polisakarida ekstraseluler (PSE) melalui proses glikosilasis. Salah satu faktor virulensi bakteri Streptococcus mutans sebagai penyebab karies gigi adalah gengtf-B Streptococcus mutans. Tujuan: Penelitian ini bertujuan untuk mendeteksi gen gtf-B Streptococcus mutans dalam plak dengan gigi karies pada anak di SD Negeri 29 Dangin Puri.Metode: Penelitian ini menggunakan 51 sampel anak di SD Negeri 29 Dangin Puri yang mengalami karies. Kultur bakteri digunakan untuk mendeteksi koloni Streptococcus Sp. kemudian dilakukan pengecatan gram, uji katalase untuk membedakan Streptococcus dengan Staphylococcus. Hasil uji katalase negatif dilakukan proses PCR Streptococcus Mutans dengan ukuran 517 bp dan gen gtf B Streptococcus mutans dengan ukuran 585 bp.Hasil dan Simpulan: ditemukan bakteri streptococcus mutans sebanyak 19 sampel dari 51 sampel (37,25%). Tiga sampel dari 19 isolat bakteri streptococcus mutans terdeteksi gen gtfB streptococcus mutans (16%). ]]>
