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Praja, Ratih Novita
Universitas Airlangga
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Serodeteksi Brucellosis dengan Metode Rose Bengal Test dan Complement Fixation Test pada Sapi Perah di Banyuwangi Wilujeng, Estiary; Suwarno, Suwarno; Praja, Ratih Novita; Hamid, Iwan Sahrial; Yunita, Maya Nurwartanti; Wibawati, Prima Ayu
Jurnal Medik Veteriner Vol. 3 No. 2 (2020): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol3.iss2.2020.188-195

Abstract

Tujuan penelitian adalah untuk mendeteksi adanya antibodi dari Brucella pada ternak sapi perah betina di Banyuwangi menggunakan uji Rose Bengal Test (RBT) untuk uji cepat dan dikonfirmasi dengan menggunakan Complement Fixation Test (CFT). Sebanyak 84 sampel dari 516 sapi perah betina di Banyuwangi, kemudian diambil secara acak. Sampel yang diambil berasal dari serum sapi perah betina. Hasil penelitian ditemukan dua sampel yang dinyatakan positif Brucellosis dengan uji RBT dan CFT. RBT merupakan uji screening yang dapat memberikan hasil positif palsu, dan kemudian dikonfirmasi dengan uji CFT untuk melihat titer hasil vaksin atau infeksi alam. Hasil positif pada uji RBT memiliki hubungan dengan hasil titer CFT karena titer antibodi pada RBT dapat diukur dari derajat aglutinasi yang terbentuk. Hasil seropositif penyakit Brucellosis di Banyuwangi sebesar 2%.
Detection of Brucellosis in Dairy Cattle in Turen District Malang Regency using Rose Bengal Test (RBT) and Complement Fixation Test (CFT) Methods Kusumastuti, Imbi; Tyasningsih, Wiwiek; Praja, Ratih Novita; Suwarno, Suwarno; Yunita, Maya Nurwartanti; Yudhana, Aditya
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.42-47

Abstract

The purpose of this research was to detect Brucellosis in female dairy cattle in Turen District, Malang Regency. A total of 78 samples were used in this research taken from 351 total population of female dairy cattle that have been calculated based on the slovin formula. The methods used in this research were Rose Bengal Test (RBT) for the screening test and continued with Complement Fixation Test (CFT) for the confirmation test. The results RBT of this research clearly showed that positive results from 10 samples were confirmed as a final diagnostic test of Brucellosis using CFT. CFT showed a positive result from 9 samples and negative in 1 sample. The percentage of Brucellosis occurrence in the Turen District was recorded at 11.5%. Moreover, Turen District also became an area with a high Brucellosis occurrence (>2%).
Detection of Antibodies Brucella abortus in dairy cattle in Puspo District, Pasuruan using Rose Bengal Test and Complement Fixation Test Kusuma, Agung Jati; Safitri, Erma; Praja, Ratih Novita; Tyasningsih, Wiwiek; Yunita, Maya Nurwartanti; Wibawati, Prima Ayu
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.199-206

Abstract

Brucellosis was an infectious disease caused by the genus brucella. Brucellosis in dairy cattle was caused by Brucella abortus that impaction an abortion. The aim of this study was to determine the presence of antibodies of the Brucella abortus in adult female dairy cattle in the Puspo district Pasuruan using Rose Bengal Test (RBT) and Complement Fixation Test (CFT). A descriptive analysis method with quantitative approach was used in this study. Meanwhile, interviews were conducted to get supporting information from the farmer. Used 100 samples. The results of the study showed 2% samples considered positive brucellosis tested by RBT. To confidence false-positive result, RBT test was followed by CFT. After tested by CFT, in this study there were no brucella antibodies in adult female dairy cattle in Puspo district, Pasuruan.
Lime Peel Liquid (Citrus aurantifolia, Swingle) Inhibit Escherichia Coli In Vitro Sari, Devi Indah; Wahjuni, Retno Sri; Praja, Ratih Novita; Utomo, Budi; Wibawati, Prima Ayu
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.63-71

Abstract

The purpose of this study was to determine in vitro inhibition test of lime peel liquid (Citrus aurantifolia, Swingle) against E. coli. The E. coli isolate was cultured on Muller Hinton Agar (MHA) media. Antibacterial test in this study using diffusion method. Negative control (K-) using CMC-Na 0.1%. Positive control (K+) using oxytetracycline antibiotics. The treatments were given lime peel liquid (Citrus aurantifolia, Swingle) with concentrations of 100%, 75%, 50% and 25%, respectively. The study design using Completely Randomized Design (CRD) method, with six treatments and four repetitions. Data were analyzed by Kruskal-Wallis test, Moreover, the Post Hoc Mann-Whitney method. The inhibition zone analysis results were showed that K + had significant differences compared to all treatments K-, 100%, 75%, 50%, and 25%. K- was found to be significantly different from treatments 100%, 75%, 50% and 25%. The results showed that the lime peel liquid has the potential as an antibacterial activity against the growth of E. coli in vitro. Concentration of 25% has shown the presence of inhibitory zones on Mueller Hinton media that have been inoculated with lime peel liquid.
Most Probable Number of Escherichia Coli in Fresh Milk at KPSP Ijen Makmur, Licin Sub-District, Banyuwangi Wijaya, Achmad Arby; Hamid, Iwan Sahrial; Yunita, Maya Nurwartanti; Tyasningsih, Wiwiek; Praja, Ratih Novita
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.207-212

Abstract

The study aimed to identify the total number of E. coli used in fresh cow milk in KPSP Ijen Makmur. The sample was used by as many as 16 samples from a group of cattle farmers. E. coli analyzed using Most Probable Number (MPN), 3 series of tubes. Before continuing the MPN test the milk must be diluted, 25 ml of milk was poured into the dilution of the 225ml peptone water buffered then homogenized for 2 minutes. MPN consist of presumptive coliform test if positive was found of gas and was cloudy, presumptive faecal coliform test positive was found in the gas and was cloudy, confirmed E.coli test if there was a black colony with or metallic green, continued by a biochemical test with red ring-positive Indole, Methyl Red positive the color is red, negative Voges Paskauer if there is no change in color, and negative citrate will turn green. Results showed that from the 16 samples of fresh milk used there were 7 samples of fresh milk that exceeded the contamination limit E. coli or < 3 apm /ml. Conclusion, number of E. coli in fresh milk at KPSP Ijen Makmur 43.75% of the total sample exceeded maximum contamination.
Necrosis Description of Mice Liver Induced with Monosodium Glutamate and Methanol Robusta Coffee Bean Extract (Coffea canephora) Al-Zuhroh, Tisya Yumn; Santoso, Kuncoro Puguh; Yunita, Maya Nurwartanti; Hidajati, Nove; Praja, Ratih Novita
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.213-220

Abstract

This study aimed to analyze the effect of methanol robusta coffee bean extract (Coffea canephora) on liver histopathological description in mice induced with monosodium glutamate (MSG). This study was used 20 male mice for 5 treatment groups: K + (MSG 0.12 mg and Vitamin C 6 mg), K- (MSG 0.12 mg and CMC Na 0.1 ml), P1 (MSG 0.12 mg and RCE 0.1 mg), P2 (MSG 0.12 mg and RCE 0.2 mg), and P3 (MSG 0.12 mg and RCE 0.4 mg) per orally for 42 days. Liver histological scores were analyzed using Kruskal Wallis test. The results of Kruskal Wallis test scored p=0,391 which indicates that the data is not significant (p>0.05). In the control and treatment groups there were no significant. In conclusion, the giving methanol robusta coffee bean extract did not show a decrease in hepatosite damage that was different from the negative control treatment group.
The Effectiveness of Ethanol Extract of Gamal Leaves (Gliricidia sepium) on Ascaridia galli Mortality in Vitro Sandy, Berliana Dwi Nandita; Suprihati, Endang; Yudhana, Aditya; Hastutiek, Poedji; Wibawati, Prima Ayu; Praja, Ratih Novita
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.82-87

Abstract

This study aimed to determine the anthelmintic activity of the ethanol extract of gamal leaves (Gliricidia sepium) against Ascaridia galli in vitro. There were six treatment groups and each consisted of four replicates. This study used ten A. galli in each treatment for all replications. Observation and recording of death of A. galli was carried out at the 2nd, 4th, 6th, 8th, 10th and 12th hours. Death of A. galli was declared if there was no movement when clamped with anatomical tweezers. The data obtained were analyzed using the ANOVA test and continued with Duncan's test. The results showed that the ethanol extract of gamal leaves had an anthelmintic effect on A. galli worms in vitro. The extract with a concentration of 5% has almost the same anthelmintic properties as piperazine citrate 10 mg/ml. In conclusion, the higher the concentration of the extract, the higher the anthelmintic properties.
Risk Factors of Parvovirus in Dogs at De' Lab Pet Clinic, Bekasi Harelas, Genoveva Callista; Arimbi, Arimbi; Hamid, Iwan Sahrial; Praja, Ratih Novita; Yunita, Maya Nurwartanti
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.170-177

Abstract

This study aimed to determine the percentage of parvovirus, risk factor analysis, and describe the symptoms and treatment of parvovirus. The study was conducted by surveying, collecting secondary data from patient medical records and Bekasi City's rainfall data, data recording, data unification, and data analysis. Data analysis was presented descriptively and tested using Chi-Square and Odds Ratio (OR). The results shows the percentage of parvovirus was 2%. The rainfall factor <150mm/month significant to the risk of parvovirus (p<0,05) and increase the risk of parvovirus 0,154 times. The age factors 0-6 month significant to the risk of parvovirus (p<0,05) and increase the risk of parvovirus 0,017 times. The breed factors not significant to the risk of parvovirus (p>0,05) and don't increase the risk of parvovirus. The main symptoms seen in parvovirus include vomiting, diarrhea especially bloody diarrhea, and fever. The main components treatments are fluid therapy, antibiotic, antiemetic, and nutritional support. The administration of other drugs can be given to dogs whose administration must be adjusted to their respective conditions.
The Effect of The Gamal Leaf Ethanol Extract on The Growth of Escherichia coli Isolated from Semi Natural Sea Turtle Nest in Boom Beach, Banyuwangi Hutahaean, Ryan David Pandapotan; Meles, Dewa Ketut; Praja, Ratih Novita; Rahmahani, Jola; Wibawati, Prima Ayu
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.225-233

Abstract

The aim of this study was to identify the antibacterial activity potention of gamal leaf (Gliricidia sepium) ethanol extract against Escherichia coli growth which isolated from Olive Ridley Sea Turtle (Lepidochelys olivacea) semi-natural nest in Boom beach, Banyuwangi. The antibacterial test used disk diffusion method. Oxytetracycline 30 µg (Oxoid CT0041B) was used as the Positive Control (K+). CMC Na 0,5% was used as the Negative Control (K-). The treatment (P1, P2, P3, P4) used various concentrations of gamal leaves ethanol extract, which is 40%, 50%, 60%, and 70%. The study assigned six treatments and four replication. The obtained data were analyzed by ANOVA, then continued with the DUNCAN test if the results showed significant differences (p<0,05). The inhibition result of positive control (K+) was 24,70c ± 7.04; and the negative control was 0,00a ± 0,00. All extract concentration result different inhibition, concentration 1 (P1) was 10,41 b ± 3,10; concentration 2 (P2) 8,14 b ± 0,45; concentration 3 (P3) 8,08 b ± 0,47; and concentration 4 (P4) 8,01b ± 0,29. These results indicated that the gamal leaves ethanol extract has antibacterial activity to inhibit the growth of E. coli isolated from Olive Ridley semi-natural nest in Boom beach, Banyuwangi.
Risk Factors for Feline Otitis in Madiun: A Prospective Study Khasana, Uswatun; Meles, Dewa Ketut; Praja, Ratih Novita; Tyasningsih, Wiwiek; Wibawati, Prima Ayu
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.29-34

Abstract

This study aimed to determine the risk factors in cats associated with the incidence of otitis. A total of 169 cat samples were evaluated by filling out questionnaires and interviewing owners, then an examination was carried out to determine symptoms and treatment so that appropriate action could be taken. Data were analyzed descriptively and tested using Chi-Square analysis. As a result, there were 26 (15,4%) cats diagnosed with otitis. The results of the analysis of significant risk factors include bathing frequency, ear hygiene, and maintenance patterns that can increase the incidence of otitis. The most common clinical symptoms are pruritus, head shaking and accompanied by earwax. The description of the statement about otitis concluded that as many as 70 cat owners, most of them did not understand otitis.
Co-Authors Agnes Theresia Soelih Estoepangestie Agung, Arya Agustono, Bodhi Al-Madinah, Wardatul Qoryah Al-Zuhroh, Tisya Yumn Aliyyah Revinda Bima Putri Alta Safina Azzahra Amalia Sofi Ramadanti Amelia, Ezanti Nur Anastasya, Anastasya Andini, Zahrotul Annerda Yurika Arend Stam Arifin, Khoirul Arimbi Audina Putri Geraldine Aziz, Githa Nurma Baroroh, Diana Nova Boedi Setiawan Bryceton Bryant The Budi Utomo Chusniati, Sri Citra Kurnia Putri Diana Agustina Diansanto Prayoga Didik Handijatno Dilla Chelsea Aziizahrani Santoso Dwi, Welyanto Kurniawan Endang Suprihati Epy Muhammad Luqman Erma Safitri Evelyn Zalfa Winni Kusuma Fachrur Rozi Faisal Fikri Faizah, Ayu Nur Farezi, Reza Adrio Farhan, Muhammad Riesta Febriana, Melati Octavia Firmansyah, Januar Fitri Khairunisa Fransiska Okta Zania Fransiska, Eunike Melanda Haditanojo , Wiyanto Haditanojo, Wiyanto Hairi, Farizah Mohd Hamonangan, Jonathan Mark Harelas, Genoveva Callista Hayati, Laila Nur Herdiansyah, Akbar Dimas Hutahaean, Ryan David Pandapotan Ichi Arum Galerani Indriyani, Dwi Putri Insani, Alivia Khairina Iwan Sahrial Hamid Iwan Sahrial Hamid Izzato Millati Jayanti Dian Eka Sari, Jayanti Dian Jihan Annisa Kartikasari, Anjani Marisa Kenconojati, Hapsari Khasana, Uswatun Kusnoto Kusnoto, Kusnoto Kusuma, Agung Jati Kusumastuti, Imbi Lestari, Dewi Sri Mafruchati, Maslichah Mandagi, Ayik Mirayanti Masyitoh, Herlina Meles, Dewa Ketut Mufasirin Muhammad Ivan Maulana Muhammad Thohawi Elziyad Purnama Muhammad, Abdul Latif Mumtazi, Fauzan Muslimah, Bintang Nesa Putri Candra Cahyani Nor, Roslindawani Md Nove Hidajati Nurfaizah, Diza Ulya Nurwartanti, Maya Nusdianto Triakoso Poedji Hastutiek Praja, Shifa Salsabilla Prameswari, Nindya Pradnya Prameswari, Ratna Ajeng Prasetyo, Dhenatra Rifqy Prastiya, Ragil Angga Pudji Srianto Puspikawati, Septa Indra Putri, Firrial Eksa Maulidania Rahmahani, Jola Ramadhan, Alfian Dzaka Fadhil Ramadhanny, Syahrul Novenda Ratna Damayanti Reza Adrio Farezi Rofiqi, Mohammad Miftahur Romadhona, Ma'rifatunnisa' Ryan Adi Taufiqurrahman Sabitha Helmi Sadhya turahmi Saksono, Bayu Saliluddin, Suhainizam Bin Muhamad Sandy, Berliana Dwi Nandita Santoso, Kuncoro Puguh Saputro, Amung Logam Sari, Devi Indah Satria, Rafif Galih Sekarsari, Dara Ayu Pramesti Setiawan Koesdarto Setiawati, Puji Aris Shifa Salsabilla Praja Siti Mufidah Siti Nur Hilmin Soetojo Soetojo Solikhah, Tridiganita Intan Sri Pantja Madyawati Sukarno, Gerda Sunarso, Agus Suroiyah, Fifi Anik Suryanie Sarudji Susilo, Rahadian Indarto Suwarno Suwarno Syifa'ul Lailiyah Tiara Wandira Hariyanto Tri Lestari Tri Wahyu Suprayogi Tyasningsih, Wiwiek Wahjuni, Retno Sri Warsito, Sunaryo Hadi Wayan Ari wijaya Wibawati, Prima Ayu Wijaya, Achmad Arby Wilujeng, Estiary Wira Tirta Jaladara Yudhana, Aditya Yulianti, Yunika Tri Yumna, Raissa Amelia Yunika Tri Yulianti Yunita, Maya Nurwartanti Zahrudin, Elham