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All Journal Media Kedokteran Hewan Jurnal Medik Veteriner Journal of Public Health Research and Community Health Development (JPH RECODE) Jurnal Layanan Masyarakat (Journal of Public Service) Media Gizi Kesmas LOSARI: Jurnal Pengabdian Kepada Masyarakat journal of Basic Medical Veterinary Jurnal Kedokteran Hewan Health Dynamics Journal of Aquaculture Science
Praja, Ratih Novita
Universitas Airlangga
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Medicine & Pharmacology Nursing Public Health Social Sciences Transportation Veterinary Other

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Hatchability Success Rate of Olive Ridley Sea Turtle (Lepidochelys olivacea) Eggs Using the INTAN BOX Incubation Device on Pulau Santen Beach, Banyuwangi Yudhana, Aditya; Sari, Jayanti Dian Eka; Praja, Ratih Novita; Herdiansyah, Akbar Dimas; Nurfaizah, Diza Ulya; Farezi, Reza Adrio; Muslimah, Bintang; Puspikawati, Septa Indra; Kenconojati, Hapsari; Soetojo, Soetojo; Susilo, Rahadian Indarto; Mufasirin, Mufasirin; Haditanojo, Wiyanto; Saksono, Bayu; Sukarno, Gerda
Journal of Basic Medical Veterinary Vol. 14 No. 1 (2025): Journal of Basic Medical Veterinary, June 2025
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v14i1.66944

Abstract

The natural hatching success rate of olive ridley sea turtle (Lepidochelys olivacea) eggs on Pulau Santen Beach is often hindered by predator disturbances, temperature fluctuations caused by human activities, and unfavorable environmental conditions, with a success rate of only 50–70%. This study aimed to analyze the hatching success rate of olive ridley sea turtle eggs using the INTAN BOX incubation device on Pulau Santen Beach, Banyuwangi. A quantitative descriptive method was employed to observe 365 eggs relocated from four nests to the INTAN BOX over an incubation period of 50–60 days. Parameters observed included temperature, humidity, and hatching success rates. Results indicated an average hatching success rate of 86%, with individual nest success rates ranging from 83% to 89%. These rates are significantly higher than the 50–70% success rate achieved through natural hatching. The INTAN BOX provides a stable environment, protects eggs from predators, and optimizes temperature and humidity conditions, thereby enhancing hatching success. Additionally, the device enables the management of hatchling sex ratios through temperature-dependent sex determination (TSD), which is critical for sea turtle population conservation. This study concluded that the INTAN BOX is an effective innovation for olive ridley turtle conservation while also supporting local community empowerment around Pulau Santen Beach.
Identifikasi Salmonella sp. pada Susu Segar di Peternakan Sapi Perah Kecamatan Licin Kabupaten Banyuwangi Satria, Rafif Galih; Hamid, Iwan Sahrial; Wibawati, Prima Ayu; Estoepangestie, Agnes Theresia Soelih; Saputro, Amung Logam; Praja, Ratih Novita
Media Kedokteran Hewan Vol. 32 No. 3 (2021): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v32i3.2021.114-118

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui tingkat cemaran bakteri Salmonella sp. pada susu segar dari peternakan sapi perah di kecamatan licin kabupaten banyuwangi. Sampel susu segar di ambil pada april 2021 sebanyak 16 sampel dari KPSP Ijen makmur di kecamatan Licin Kabupaten Banyuwangi. Sampel diambil sebanyak 25 ml dari masing-masing peternak. Hasil yang di dapat setelah melakukan isolasi dan identifikasi menggunakan media Salmonella Shigella Agar (SSA), dan dilanjutkan uji identifikasi menggunakan TSIA dan Urea didapatkan  dari ke 16 sampel semua dinyatakn negatif bakteri Salmonella sp.. Hal ini dapat terjadi karena para peternak sapi perah di Kecamatan licin sudah mulai menyadari pentingnya kebersihan kandang dan sanitasi kandang yang baik sehingga tingkat cemaran bakteri Salmonella sp. dapat dicegah.
First case report of Salmonella spp. infection in Homalopsis buccata snake: Laporan Pertama Kasus Infeksi Salmonella spp. pada Ular Homalopsis buccata Tiara Wandira Hariyanto; Amelia, Ezanti Nur; Tyasningsih, Wiwiek; Rahmahani, Jola; Praja, Ratih Novita; Yudhana, Aditya; Nurwartanti, Maya; Fikri, Faisal
Media Kedokteran Hewan Vol. 34 No. 2 (2023): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v34i2.2023.128-134

Abstract

Reptiles are a source of transmission of pathogenic bacteria to humans. Salmonella spp. are Gram-negative bacteria that are commonly found in the digestive tract of snakes. This study was conducted on Homalopsis buccata snakes with samples obtained by oral swabs and cloacal swabs on snakes. Isolation of Salmonella spp. carried out on Tetrathionate Broth media enrichment and will be inoculated on specific media in the form of Salmonella Shigella Agar media. Identification of bacteria was carried out by Gam staining and Indole, Methyl Red, Voges Proskauer and Citrate test. This study revealed the presence of Salmonella spp. in the snake Homalopsis buccata.
MOLECULAR DETECTION OF GENE RESISTANT TO VARIOUS INSECTICIDES IN Aedes aegypti AT BANYUWANGI EAST JAVA USING POLYMERASE CHAIN REACTION Yudhana, Aditya; Praja, Ratih Novita; Yunita, Maya Nurwartanti
Jurnal Kedokteran Hewan Vol 12, No 2 (2018): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i2.7625

Abstract

The purpose of this study was to determine the type of insecticides which Aedes aegypti mosquitoes are resistant to, so that other susceptibleinsecticides still can be used. The study was a cross sectional epidemiological study with cluster sampling in Sub-district Wongsorejo,Banyuwangi, Muncar, Tegaldlimo, Kalibaru which is considered sufficient to represent Banyuwangi Regency. Mosquito samples appropriate tothe characteristics were isolated and tested for insecticide-resistance primer namely voltage gated sodium channel (VGSC) to determine thespecific resistance expressed in mosquitoes. The result of resistance test using WHO standard method showed that Aedes aegypti mosquito fromBanyuwangi Regency were resistant to cypermethrin 0.25% and malathion 0.8%. There was a VGSC coding gene with 250 bp band detectedusing polymerase chain reaction (PCR) technique which was associated with the resistance of Aedes aegypti mosquitoes in Banyuwangi Regencyto organophosphate insecticides (malathione) and pyrethroid insecticides (cypermethrin).
CHARACTERIZATION OF VirB4 PROTEIN OF LOCAL ISOLATE Brucella abortus WITH WESTERN BLOTTING TECHNIQUE Praja, Ratih Novita; Handijatno, Didik; Koesdarto, Setiawan; Yudhana, Aditya
Jurnal Kedokteran Hewan Vol 12, No 1 (2018): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i1.8091

Abstract

This research aimed to characterize VirB4 protein of local isolate Brucella abortus with Western blotting method. The result showed that there were four protein bands with molecular weights of 64.61, 59.25, 21.63, and 16.70 kDa by triggering a reaction between the whole Brucella abortus and anti-Brucella abortus serum. The results also revealed that there was only one protein band with a molecular weight of 59.25 kDa triggering a reaction between the whole Brucella abortus and anti-VirB Brucella abortus serum. Finally, it can be concluded that VirB4 protein can affect the virulence factor of Brucella abortus, successfully characterized with the appearance of one band with a molecular weight of 59.25 kDa by using Western blotting method.
Gerakan Zero Waste Melalui Penyehatan Pantai, Pembangunan Greenhouse Ecobrick, dan Pelestarian Ekosistem Laut dalam Upaya Mewujudkan SDGs 2030 Jayanti Dian Eka Sari; Syifa'ul Lailiyah; Diansanto Prayoga; Izzato Millati; Ayik Mirayanti Mandagi; Faisal Fikri; Ratih Novita Praja; Diana Agustina; Siti Nur Hilmin; Yunika Tri Yulianti
Media Gizi Kesmas Vol 12 No 2 (2023): MEDIA GIZI KESMAS (DECEMBER 2023)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mgk.v12i2.2023.802-809

Abstract

Background: Banyuwangi Regency is one of the regions in Indonesia which is rich in beach tourism potential, one of which is Pulau Santen Beach which is located in Karangrejo Village, Banyuwangi District, Banyuwangi Regency. However, when the charm of this destination has decreased, the condition of the beach is dirty if left unchecked and no coastal vegetation is carried out, it can damage the marine biota in the vicinity. There is a need for a Santen Island community empowerment program that reviews conservation and sustainable use in order to meet the 14th Sustainable Development Goals (SDGs). Objectives: Community Service entitled "Zero Waste Movement through Beach Environment Sanitation, Greenhouse Ecobrick Development, and Marine Ecosystem Preservation" aims to increase the knowledge, skills and economic independence of the Santen Island community regarding hiding santigi plants, MSME marketing strategies in tourist attractions, and waste management through making ecobricks. Methods: The method used for the implementation of community service is through several stages including, the first stage in this community service program is the preparatory stage. This preparatory stage consists of three steps, namely an environmental survey, purchasing the right sources, and planning a community service program. The second stage of this community service program is the implementation stage and the third advanced stage of this community service program is evaluation. Results: On the first day, there was an increase in the knowledge of the Santen Island community by 18.8%. On the second day there was an increase in public knowledge of 2.2%, and on the third day with ecobrick material there was an increase in public knowledge of 20.2%. This shows that the material can be well captured by the target of community service activities. Conclusions: This activity is later expected to be a provision for the community to revive the tourism potential on Santen Island, namely edutourism which includes aspects of knowledge about cultivating santigi plants, healthy coastal environments through processing inorganic waste into goods of high economic value (ecobricks) and community economic independence through increasing financial capacity new tourism products for edutourism on Santen Island.
Antibacterial Potential of Bajakah Tampala Root Extract (Spatholobus littoralis Hassk.) Against the Growth of Staphylococcus aureus Nesa Putri Candra Cahyani; Iwan Sahrial Hamid; Epy Muhammad Luqman; Maya Nurwartanti Yunita; Ratih Novita Praja
Journal of Basic Medical Veterinary Vol. 15 No. 1 (2026): Journal of Basic Medical Veterinary, June 2026
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v15i1.88612

Abstract

Staphylococcus aureus infections remain a significant problem in human and veterinary medicine, particularly due to the increasing prevalence of antibiotic resistance. The use of natural materials as alternative antibacterial agents is a potential solution. This study aimed to determine the antibacterial activity of bajakah tampala root extract (Spatholobus littoralis Hassk.) against the growth of Staphylococcus aureus in vitro. This study was a laboratory experimental study using a post-test-only control group design. Bajakah tampala root extract (Spatholobus littoralis Hassk.) was prepared at concentrations of 25%, 50%, 75%, and 100%. Antibacterial activity was evaluated using the disc diffusion method on Mueller–Hinton Agar (MHA). Erythromycin was used as a positive control. The observed parameter was the diameter of the inhibition zone after 24 hours of incubation at 37°C. Data were analyzed using a one-way ANOVA test. The results showed that bajakah tampala root extract (Spatholobus littoralis Hassk.) inhibited the growth of Staphylococcus aureus at all tested concentrations. The diameter of the inhibition zone increased with increasing extract concentration, with the 100% concentration producing the greatest inhibition. Statistical analysis showed a significant difference between the treatment groups and the control (p < 0.05).
Application of Probiotics Based on Immunostimulant β-Glucan Saccharomyces cerevisiae Combination of Antibacterial Sonneratia alba Leaves as Vibrio harveyi Inhibitor of White Shrimp (Litopenaeus vannamei) Alta Safina Azzahra; Siti Mufidah; Sadhya turahmi; Bryceton Bryant The; Ratih Novita Praja, drh., M. Si
Journal of Aquaculture Science Vol 9 No 2 (2024): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/joas.v9i2.61942

Abstract

Vibrio harveyi can cause mass death in vaname shrimp. The β-Glucan content in Saccharomyces cerevisiae can stimulate the immune system non- specifically. Meanwhile, the endophytic bacteria Sonneratia alba has antibacterial activity. This study aims to determine the effectiveness of the probiotic fee formulation of β Glucan S. cerevisiae using a combination of antibacterial S. alba leaves as an effort to inhibit the growth of V. harveyi in vaname shrimp (L. vannamei). The research method used is an experimental probiotic formulation from a combination of β Glucan S. cerevisiae and endophytic bacteria S. alba through shrimp feed with 6 treatments and 4 replications, The parameters used are Total Haemocyte Count, Differential Haemocyte Count and Survival Rate. This study was conducted in vivo tests for 10 days of probiotic feed maintenance and 7 days of infection with a total research period of four months at the Faculty of Health, Medicine, and Natural Sciences, Airlangga. The results of the research show that the combination of ingredients can inhibit the growth of V. harveyi through the immune response and survival of vannamei shrimp. The best treatment was P3 with a Total Haemocyte Count (THC) of 7,54x106 cells/ml and Differential Haemocyte Cout (DHC) (60,56% granular, 16,68% semi-granular, 22,76% hyaline) with 85% survival. The provision of probiotics had a real effect and became the optimum formulation to inhibit the growth of V. harveyi.
Optimized Dunaliella salina-Salvinia molesta Probiotic Feed Enhances Hemocyte-Mediated Immunity of Litopenaeus vannamei under Taura Syndrome Virus Challenge Ryan Adi Taufiqurrahman; Reza Adrio Farezi; Fitri Khairunisa; Sabitha Helmi; Ratih Novita Praja; Arend Stam
Journal of Aquaculture Science Vol 11 No 1 (2026): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/joas.v11i1.82312

Abstract

Taura Syndrome Virus (TSV) is one of the diseases that is capable of causing mass mortality ranging from 40% to 90% during the post-larval and juvenile stages of vannamei shrimp. The prevention of viral diseases in shrimp generally relies on antibiotics or vaccines, but it's still not effective. The use of probiotic feed containing extracts from Dunaliella salina and Salvinia molesta can enhance shrimp immunity. This research aims to determine the optimization of D. salina and S. molesta carotenoids as an effective alternative for preventing Taura Syndrome Virus (TSV) based on in vivo test results. The methods used for this research are carotenoids from D. salina, which were extracted at peak density (day 6) using solvent extraction, while β-carotene was extracted by maceration using n-hexane for 24 hours under dark conditions. The obtained extract was stabilized with NaHCO₃ and further processed for microencapsulation. Biomass was Shrimp were given various feed formulations: F1 (conventional feed + injected with PBS solution 0.1 ml), F2 (conventional feed), F3 (1:1), F4 (1:2), F5 (2:1), all groups were exposed to 0.1 ml TSV virus, except F1.  Therefore, F4 was selected as the best formulation, as it was most effective in improving growth performance and survival rate, as well as acting as an immunostimulant by increasing hyaline, granular, and semi-granular cells based on in vivo test results.
Pentahelix Approach in Community Empowerment-Based Ecotourism: Turtle Conservation and Waste Management on Santen Island Beach, Banyuwangi Jayanti Dian Eka Sari; Aditya Yudhana; Melati Octavia Febriana; Syahrul Novenda Ramadhanny; Firrial Eksa Maulidania Putri; Suhainizam Bin Muhamad Saliluddin; Ratih Novita Praja
Health Dynamics Vol 1, No 12 (2024): December 2024
Publisher : Knowledge Dynamics

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hd11205

Abstract

Background: Santen Island Beach, Banyuwangi, faces significant challenges from waste mismanagement and its impact on marine ecosystems, particularly the endangered sea turtles. This study addresses these issues through a community empowerment program grounded in the Pentahelix framework, engaging stakeholders from government, academia, businesses, communities, and media. The program, active since 2022, aims to integrate sustainable waste management and turtle conservation into community development. Methods: The study employed a community empowerment approach, incorporating waste segregation, educational tourism initiatives, and turtle conservation training. Collaborations with local organizations like the Banyuwangi Sea Turtle Foundation and waste management entities ensured resource and expertise mobilization. Activities included waste alms programs and sea turtle egg relocation to sandless hatching boxes, supplemented by training and social media campaigns. Results: The program achieved key milestones, such as forming empowered cadres for waste management and turtle conservation, introducing effective waste segregation practices, and increasing hatchling survival rates through innovative relocation techniques. Outputs included community-led eco-tourism branding, scientific and media publications, and economic benefits for local stakeholders. Conclusion: This initiative highlights the potential of integrating environmental conservation and economic empowerment within a sustainable development framework. By leveraging the Pentahelix model, the program fosters local ownership and long-term impact, addressing SDG goals related to responsible consumption and marine ecosystem preservation. Continued efforts, such as enhancing cadre competencies and optimizing ecotourism branding, are recommended for sustained outcomes.
Co-Authors Agnes Theresia Soelih Estoepangestie Agustono, Bodhi Al-Madinah, Wardatul Qoryah Al-Zuhroh, Tisya Yumn Aliyyah Revinda Bima Putri Alta Safina Azzahra Amalia Sofi Ramadanti Amelia, Ezanti Nur Anastasya, Anastasya Andini, Zahrotul Annerda Yurika Arend Stam Arifin, Khoirul Arimbi Arya Agung Audina Putri Geraldine Aziz, Githa Nurma Baroroh, Diana Nova Boedi Setiawan Bryceton Bryant The Budi Utomo Chusniati, Sri Citra Kurnia Putri Diana Agustina Diansanto Prayoga Didik Handijatno Dilla Chelsea Aziizahrani Santoso Dwi, Welyanto Kurniawan Endang Suprihati Epy Muhammad Luqman Erma Safitri Evelyn Zalfa Winni Kusuma Fachrur Rozi Faisal Fikri Faizah, Ayu Nur Farezi, Reza Adrio Farhan, Muhammad Riesta Firmansyah, Januar Firrial Eksa Maulidania Putri Fitri Khairunisa Fransiska Okta Zania Fransiska, Eunike Melanda Haditanojo , Wiyanto Haditanojo, Wiyanto Hairi, Farizah Mohd Hamonangan, Jonathan Mark Harelas, Genoveva Callista Hayati, Laila Nur Herdiansyah, Akbar Dimas Hutahaean, Ryan David Pandapotan Ichi Arum Galerani Indriyani, Dwi Putri Insani, Alivia Khairina Iwan Sahrial Hamid Iwan Sahrial Hamid Izzato Millati Jayanti Dian Eka Sari, Jayanti Dian Jihan Annisa Kartikasari, Anjani Marisa Kenconojati, Hapsari Khasana, Uswatun Kusnoto Kusnoto, Kusnoto Kusuma, Agung Jati Kusumastuti, Imbi Lestari, Dewi Sri Mafruchati, Maslichah Mandagi, Ayik Mirayanti Masyitoh, Herlina Melati Octavia Febriana Meles, Dewa Ketut Mufasirin Muhammad Ivan Maulana Muhammad Thohawi Elziyad Purnama Mumtazi, Fauzan Muslimah, Bintang Nawawi, Muhammad Latif Nesa Putri Candra Cahyani Nor, Roslindawani Md Nove Hidajati Nurfaizah, Diza Ulya Nurwartanti, Maya Nusdianto Triakoso Poedji Hastutiek Praja, Shifa Salsabilla Prameswari, Nindya Pradnya Prameswari, Ratna Ajeng Prasetyo, Dhenatra Rifqy Prastiya, Ragil Angga Pudji Srianto Puspikawati, Septa Indra Rahmahani, Jola Ramadhan, Alfian Dzaka Fadhil Ratna Damayanti Reza Adrio Farezi Rofiqi, Mohammad Miftahur Romadhona, Ma'rifatunnisa' Ryan Adi Taufiqurrahman Sabitha Helmi Sadhya turahmi Saksono, Bayu Sandy, Berliana Dwi Nandita Santoso, Kuncoro Puguh Saputro, Amung Logam Sari, Devi Indah Satria, Rafif Galih Sekarsari, Dara Ayu Pramesti Setiawan Koesdarto Setiawati, Puji Aris Shifa Salsabilla Praja Siti Mufidah Siti Nur Hilmin Soetojo Soetojo Solikhah, Tridiganita Intan Sri Pantja Madyawati Suhainizam Bin Muhamad Saliluddin Sukarno, Gerda Sunarso, Agus Suroiyah, Fifi Anik Suryanie Sarudji Susilo, Rahadian Indarto Suwarno Suwarno Syahrul Novenda Ramadhanny Syifa'ul Lailiyah Tiara Wandira Hariyanto Tri Lestari Tri Wahyu Suprayogi Tyasningsih, Wiwiek Wahjuni, Retno Sri Warsito, Sunaryo Hadi Wayan Ari wijaya Wibawati, Prima Ayu Wijaya, Achmad Arby Wilujeng, Estiary Wira Tirta Jaladara Yudhana, Aditya Yulianti, Yunika Tri Yumna, Raissa Amelia Yunika Tri Yulianti Yunita, Maya Nurwartanti Zahrudin, Elham