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All Journal Media Kedokteran Hewan Jurnal Medik Veteriner Journal of Public Health Research and Community Health Development (JPH RECODE) Jurnal Layanan Masyarakat (Journal of Public Service) Media Gizi Kesmas LOSARI: Jurnal Pengabdian Kepada Masyarakat journal of Basic Medical Veterinary Jurnal Kedokteran Hewan Health Dynamics Journal of Aquaculture Science
Praja, Ratih Novita
Universitas Airlangga
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Medicine & Pharmacology Nursing Public Health Social Sciences Transportation Veterinary Other

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Identification of Gram-Negative Bacteria in the Oral Cavity of Homalopsis buccata Praja, Ratih Novita; Yudhana, Aditya; Amelia, Ezanti Nur; Ramadhan, Alfian Dzaka Fadhil; Zahrudin, Elham; Setiawati, Puji Aris; Yumna, Raissa Amelia; Andini, Zahrotul; Nor, Roslindawani Md
Media Kedokteran Hewan Vol. 36 No. 2 (2025): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v36i2.2025.163-172

Abstract

The kadut belang snake (Homalopsis buccata) is a semi-aquatic snake that can be aggressive when threatened. H. buccata is often used as a pet, a source of animal protein, and raw materials for leather crafts because of its relatively large size and unique pattern. The increasing trend of keeping reptiles as pets could increase the potential for transmission of bacteria to humans. The purpose of this study was to isolate and identify Aeromonas hydrophila, Escherichia coli, Klebsiella pneumoniae, and Salmonella sp. from H. buccata. This type of research uses the accidental sampling method as the research design. The H. buccata criteria used in this study came from wild catches and were imported during the study period. Isolation was carried out to obtain separate bacterial colonies and identification of bacteria was carried out by Gram staining and biochemical reaction tests. Identification of Gram-Negative Bacteria in the Oral Cavity showed that A. hydrophila, E. coli, K. pneumoniae, and Salmonella sp. with a percentage of 33.3%, 11.1%, 55.5%, and 22.2% in the digestive tract of H. buccata snakes in the Mojokerto City area.
Hatchability Success Rate of Olive Ridley Sea Turtle (Lepidochelys olivacea) Eggs Using the INTAN BOX Incubation Device on Pulau Santen Beach, Banyuwangi Yudhana, Aditya; Sari, Jayanti Dian Eka; Praja, Ratih Novita; Herdiansyah, Akbar Dimas; Nurfaizah, Diza Ulya; Farezi, Reza Adrio; Muslimah, Bintang; Puspikawati, Septa Indra; Kenconojati, Hapsari; Soetojo, Soetojo; Susilo, Rahadian Indarto; Mufasirin, Mufasirin; Haditanojo, Wiyanto; Saksono, Bayu; Sukarno, Gerda
Journal of Basic Medical Veterinary Vol. 14 No. 1 (2025): Journal of Basic Medical Veterinary, June 2025
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v14i1.66944

Abstract

The natural hatching success rate of olive ridley sea turtle (Lepidochelys olivacea) eggs on Pulau Santen Beach is often hindered by predator disturbances, temperature fluctuations caused by human activities, and unfavorable environmental conditions, with a success rate of only 50–70%. This study aimed to analyze the hatching success rate of olive ridley sea turtle eggs using the INTAN BOX incubation device on Pulau Santen Beach, Banyuwangi. A quantitative descriptive method was employed to observe 365 eggs relocated from four nests to the INTAN BOX over an incubation period of 50–60 days. Parameters observed included temperature, humidity, and hatching success rates. Results indicated an average hatching success rate of 86%, with individual nest success rates ranging from 83% to 89%. These rates are significantly higher than the 50–70% success rate achieved through natural hatching. The INTAN BOX provides a stable environment, protects eggs from predators, and optimizes temperature and humidity conditions, thereby enhancing hatching success. Additionally, the device enables the management of hatchling sex ratios through temperature-dependent sex determination (TSD), which is critical for sea turtle population conservation. This study concluded that the INTAN BOX is an effective innovation for olive ridley turtle conservation while also supporting local community empowerment around Pulau Santen Beach.
Identifikasi Salmonella sp. pada Susu Segar di Peternakan Sapi Perah Kecamatan Licin Kabupaten Banyuwangi Satria, Rafif Galih; Hamid, Iwan Sahrial; Wibawati, Prima Ayu; Estoepangestie, Agnes Theresia Soelih; Saputro, Amung Logam; Praja, Ratih Novita
Media Kedokteran Hewan Vol. 32 No. 3 (2021): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v32i3.2021.114-118

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui tingkat cemaran bakteri Salmonella sp. pada susu segar dari peternakan sapi perah di kecamatan licin kabupaten banyuwangi. Sampel susu segar di ambil pada april 2021 sebanyak 16 sampel dari KPSP Ijen makmur di kecamatan Licin Kabupaten Banyuwangi. Sampel diambil sebanyak 25 ml dari masing-masing peternak. Hasil yang di dapat setelah melakukan isolasi dan identifikasi menggunakan media Salmonella Shigella Agar (SSA), dan dilanjutkan uji identifikasi menggunakan TSIA dan Urea didapatkan  dari ke 16 sampel semua dinyatakn negatif bakteri Salmonella sp.. Hal ini dapat terjadi karena para peternak sapi perah di Kecamatan licin sudah mulai menyadari pentingnya kebersihan kandang dan sanitasi kandang yang baik sehingga tingkat cemaran bakteri Salmonella sp. dapat dicegah.
First case report of Salmonella spp. infection in Homalopsis buccata snake: Laporan Pertama Kasus Infeksi Salmonella spp. pada Ular Homalopsis buccata Tiara Wandira Hariyanto; Amelia, Ezanti Nur; Tyasningsih, Wiwiek; Rahmahani, Jola; Praja, Ratih Novita; Yudhana, Aditya; Nurwartanti, Maya; Fikri, Faisal
Media Kedokteran Hewan Vol. 34 No. 2 (2023): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v34i2.2023.128-134

Abstract

Reptiles are a source of transmission of pathogenic bacteria to humans. Salmonella spp. are Gram-negative bacteria that are commonly found in the digestive tract of snakes. This study was conducted on Homalopsis buccata snakes with samples obtained by oral swabs and cloacal swabs on snakes. Isolation of Salmonella spp. carried out on Tetrathionate Broth media enrichment and will be inoculated on specific media in the form of Salmonella Shigella Agar media. Identification of bacteria was carried out by Gam staining and Indole, Methyl Red, Voges Proskauer and Citrate test. This study revealed the presence of Salmonella spp. in the snake Homalopsis buccata.
MOLECULAR DETECTION OF GENE RESISTANT TO VARIOUS INSECTICIDES IN Aedes aegypti AT BANYUWANGI EAST JAVA USING POLYMERASE CHAIN REACTION Yudhana, Aditya; Praja, Ratih Novita; Yunita, Maya Nurwartanti
Jurnal Kedokteran Hewan Vol 12, No 2 (2018): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i2.7625

Abstract

The purpose of this study was to determine the type of insecticides which Aedes aegypti mosquitoes are resistant to, so that other susceptibleinsecticides still can be used. The study was a cross sectional epidemiological study with cluster sampling in Sub-district Wongsorejo,Banyuwangi, Muncar, Tegaldlimo, Kalibaru which is considered sufficient to represent Banyuwangi Regency. Mosquito samples appropriate tothe characteristics were isolated and tested for insecticide-resistance primer namely voltage gated sodium channel (VGSC) to determine thespecific resistance expressed in mosquitoes. The result of resistance test using WHO standard method showed that Aedes aegypti mosquito fromBanyuwangi Regency were resistant to cypermethrin 0.25% and malathion 0.8%. There was a VGSC coding gene with 250 bp band detectedusing polymerase chain reaction (PCR) technique which was associated with the resistance of Aedes aegypti mosquitoes in Banyuwangi Regencyto organophosphate insecticides (malathione) and pyrethroid insecticides (cypermethrin).
CHARACTERIZATION OF VirB4 PROTEIN OF LOCAL ISOLATE Brucella abortus WITH WESTERN BLOTTING TECHNIQUE Praja, Ratih Novita; Handijatno, Didik; Koesdarto, Setiawan; Yudhana, Aditya
Jurnal Kedokteran Hewan Vol 12, No 1 (2018): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i1.8091

Abstract

This research aimed to characterize VirB4 protein of local isolate Brucella abortus with Western blotting method. The result showed that there were four protein bands with molecular weights of 64.61, 59.25, 21.63, and 16.70 kDa by triggering a reaction between the whole Brucella abortus and anti-Brucella abortus serum. The results also revealed that there was only one protein band with a molecular weight of 59.25 kDa triggering a reaction between the whole Brucella abortus and anti-VirB Brucella abortus serum. Finally, it can be concluded that VirB4 protein can affect the virulence factor of Brucella abortus, successfully characterized with the appearance of one band with a molecular weight of 59.25 kDa by using Western blotting method.
Environmental and Management Risk Factors for Goat Mastitis in Banyuwangi Lestari, Dewi Sri; Setiawan, Boedi; Saputro, Amung Logam; Mafruchati, Maslichah; Praja, Ratih Novita; Prastiya, Ragil Angga
Journal of Basic Medical Veterinary Vol. 14 No. 2 (2025): Journal of Basic Medical Veterinary, December 2025
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v14i2.49399

Abstract

Mastitis is a persistent and economically significant health problem in dairy goats in Banyuwangi, East Java, where smallholder production systems dominate. This review synthesises current evidence on the primary risk factors contributing to the high prevalence of subclinical mastitis in the region. Local studies consistently report Staphylococcus aureus as the leading etiological agent, frequently exhibiting antimicrobial resistance linked to unsupervised antibiotic use. Host-related factors such as advanced parity, udder lesions, and teat-end damage increase susceptibility to intramammary infection. Management practices—including inadequate milking hygiene, use of shared towels, absence of pre- and post-dipping, and irregular equipment sanitation—facilitate the transmission of contagious pathogens. Environmental conditions, particularly high humidity and poor housing sanitation, further elevate infection pressure. Socio-economic constraints and limited farmer knowledge about subclinical mastitis and antibiotic stewardship exacerbate disease persistence. The multifactorial nature of mastitis in Banyuwangi underscores the need for context-appropriate interventions combining improved hygiene practices, better housing management, routine screening, and responsible antimicrobial use. Strengthened farmer education and targeted surveillance are essential to reduce mastitis prevalence and enhance the sustainability of goat dairy production in the region.
Eksplorasi (Electric Separating Sperm) ESS dalam Keberlanjutan Peningkatan Produksi dan Reproduksi Melalui Seleksi Sperma serta Penentuan Jenis Kelamin Ternak Kambing PPKDN Desa Sambirejo, Kecamatan Bangorejo, Banyuwangi Amung Logam Saputro; Ratih Novita Praja; Wayan Ari wijaya; Shifa Salsabilla Praja; Wira Tirta Jaladara; Jihan Annisa; Dilla Chelsea Aziizahrani Santoso; Evelyn Zalfa Winni Kusuma; Agung, Arya; Muhammad Ivan Maulana; Amalia Sofi Ramadanti; Annerda Yurika
LOSARI: Jurnal Pengabdian Kepada Masyarakat Vol. 8 No. 1 (2026): April 2026
Publisher : LOSARI DIGITAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53860/losari.v8i1.547

Abstract

Bangorejo District, Banyuwangi Regency, East Java, has significant potential for goat farming development; however, it still faces challenges related to productivity and reproductive efficiency due to the limited adoption of modern reproductive technologies. This community service program aimed to improve farmers’ knowledge and skills through the implementation of Electric Separating Sperm (ESS) technology. The activities were conducted through counseling sessions, training, and demonstrations of ESS application involving 30 goat farmers in Sambirejo Village. The effectiveness of the program was evaluated using pre-test and post-test methods. The results showed an increase in the number of correct responses across all question indicators (P1–P15), with an average improvement of 1–2 participants per indicator, equivalent to approximately 3.3–6.7% of the total participants. This improvement indicates enhanced farmer understanding of sperm sexing technology and livestock reproductive management. The implementation of ESS provides a simpler reproductive technology alternative with potential applicability at the smallholder farmer level to support reproductive efficiency and sustainably improve goat productivity.
Gerakan Zero Waste Melalui Penyehatan Pantai, Pembangunan Greenhouse Ecobrick, dan Pelestarian Ekosistem Laut dalam Upaya Mewujudkan SDGs 2030 Jayanti Dian Eka Sari; Syifa'ul Lailiyah; Diansanto Prayoga; Izzato Millati; Ayik Mirayanti Mandagi; Faisal Fikri; Ratih Novita Praja; Diana Agustina; Siti Nur Hilmin; Yunika Tri Yulianti
Media Gizi Kesmas Vol 12 No 2 (2023): MEDIA GIZI KESMAS (DECEMBER 2023)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mgk.v12i2.2023.802-809

Abstract

Background: Banyuwangi Regency is one of the regions in Indonesia which is rich in beach tourism potential, one of which is Pulau Santen Beach which is located in Karangrejo Village, Banyuwangi District, Banyuwangi Regency. However, when the charm of this destination has decreased, the condition of the beach is dirty if left unchecked and no coastal vegetation is carried out, it can damage the marine biota in the vicinity. There is a need for a Santen Island community empowerment program that reviews conservation and sustainable use in order to meet the 14th Sustainable Development Goals (SDGs). Objectives: Community Service entitled "Zero Waste Movement through Beach Environment Sanitation, Greenhouse Ecobrick Development, and Marine Ecosystem Preservation" aims to increase the knowledge, skills and economic independence of the Santen Island community regarding hiding santigi plants, MSME marketing strategies in tourist attractions, and waste management through making ecobricks. Methods: The method used for the implementation of community service is through several stages including, the first stage in this community service program is the preparatory stage. This preparatory stage consists of three steps, namely an environmental survey, purchasing the right sources, and planning a community service program. The second stage of this community service program is the implementation stage and the third advanced stage of this community service program is evaluation. Results: On the first day, there was an increase in the knowledge of the Santen Island community by 18.8%. On the second day there was an increase in public knowledge of 2.2%, and on the third day with ecobrick material there was an increase in public knowledge of 20.2%. This shows that the material can be well captured by the target of community service activities. Conclusions: This activity is later expected to be a provision for the community to revive the tourism potential on Santen Island, namely edutourism which includes aspects of knowledge about cultivating santigi plants, healthy coastal environments through processing inorganic waste into goods of high economic value (ecobricks) and community economic independence through increasing financial capacity new tourism products for edutourism on Santen Island.
Antibacterial Potential of Bajakah Tampala Root Extract (Spatholobus littoralis Hassk.) Against the Growth of Staphylococcus aureus Nesa Putri Candra Cahyani; Iwan Sahrial Hamid; Epy Muhammad Luqman; Maya Nurwartanti Yunita; Ratih Novita Praja
Journal of Basic Medical Veterinary Vol. 15 No. 1 (2026): Journal of Basic Medical Veterinary, June 2026
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v15i1.88612

Abstract

Staphylococcus aureus infections remain a significant problem in human and veterinary medicine, particularly due to the increasing prevalence of antibiotic resistance. The use of natural materials as alternative antibacterial agents is a potential solution. This study aimed to determine the antibacterial activity of bajakah tampala root extract (Spatholobus littoralis Hassk.) against the growth of Staphylococcus aureus in vitro. This study was a laboratory experimental study using a post-test-only control group design. Bajakah tampala root extract (Spatholobus littoralis Hassk.) was prepared at concentrations of 25%, 50%, 75%, and 100%. Antibacterial activity was evaluated using the disc diffusion method on Mueller–Hinton Agar (MHA). Erythromycin was used as a positive control. The observed parameter was the diameter of the inhibition zone after 24 hours of incubation at 37°C. Data were analyzed using a one-way ANOVA test. The results showed that bajakah tampala root extract (Spatholobus littoralis Hassk.) inhibited the growth of Staphylococcus aureus at all tested concentrations. The diameter of the inhibition zone increased with increasing extract concentration, with the 100% concentration producing the greatest inhibition. Statistical analysis showed a significant difference between the treatment groups and the control (p < 0.05).
Co-Authors Agnes Theresia Soelih Estoepangestie Agung, Arya Agustono, Bodhi Al-Madinah, Wardatul Qoryah Al-Zuhroh, Tisya Yumn Aliyyah Revinda Bima Putri Alta Safina Azzahra Amalia Sofi Ramadanti Amelia, Ezanti Nur Anastasya, Anastasya Andini, Zahrotul Annerda Yurika Arend Stam Arifin, Khoirul Arimbi Audina Putri Geraldine Aziz, Githa Nurma Baroroh, Diana Nova Boedi Setiawan Bryceton Bryant The Budi Utomo Chusniati, Sri Citra Kurnia Putri Diana Agustina Diansanto Prayoga Didik Handijatno Dilla Chelsea Aziizahrani Santoso Dwi, Welyanto Kurniawan Endang Suprihati Epy Muhammad Luqman Erma Safitri Evelyn Zalfa Winni Kusuma Fachrur Rozi Faisal Fikri Faizah, Ayu Nur Farezi, Reza Adrio Farhan, Muhammad Riesta Febriana, Melati Octavia Firmansyah, Januar Fitri Khairunisa Fransiska Okta Zania Fransiska, Eunike Melanda Haditanojo , Wiyanto Haditanojo, Wiyanto Hairi, Farizah Mohd Hamonangan, Jonathan Mark Harelas, Genoveva Callista Hayati, Laila Nur Herdiansyah, Akbar Dimas Hutahaean, Ryan David Pandapotan Ichi Arum Galerani Indriyani, Dwi Putri Insani, Alivia Khairina Iwan Sahrial Hamid Iwan Sahrial Hamid Izzato Millati Jayanti Dian Eka Sari, Jayanti Dian Jihan Annisa Kartikasari, Anjani Marisa Kenconojati, Hapsari Khasana, Uswatun Kusnoto Kusnoto, Kusnoto Kusuma, Agung Jati Kusumastuti, Imbi Lestari, Dewi Sri Mafruchati, Maslichah Mandagi, Ayik Mirayanti Masyitoh, Herlina Meles, Dewa Ketut Mufasirin Muhammad Ivan Maulana Muhammad Thohawi Elziyad Purnama Muhammad, Abdul Latif Mumtazi, Fauzan Muslimah, Bintang Nesa Putri Candra Cahyani Nor, Roslindawani Md Nove Hidajati Nurfaizah, Diza Ulya Nurwartanti, Maya Nusdianto Triakoso Poedji Hastutiek Praja, Shifa Salsabilla Prameswari, Nindya Pradnya Prameswari, Ratna Ajeng Prasetyo, Dhenatra Rifqy Prastiya, Ragil Angga Pudji Srianto Puspikawati, Septa Indra Putri, Firrial Eksa Maulidania Rahmahani, Jola Ramadhan, Alfian Dzaka Fadhil Ramadhanny, Syahrul Novenda Ratna Damayanti Reza Adrio Farezi Rofiqi, Mohammad Miftahur Romadhona, Ma'rifatunnisa' Ryan Adi Taufiqurrahman Sabitha Helmi Sadhya turahmi Saksono, Bayu Saliluddin, Suhainizam Bin Muhamad Sandy, Berliana Dwi Nandita Santoso, Kuncoro Puguh Saputro, Amung Logam Sari, Devi Indah Satria, Rafif Galih Sekarsari, Dara Ayu Pramesti Setiawan Koesdarto Setiawati, Puji Aris Shifa Salsabilla Praja Siti Mufidah Siti Nur Hilmin Soetojo Soetojo Solikhah, Tridiganita Intan Sri Pantja Madyawati Sukarno, Gerda Sunarso, Agus Suroiyah, Fifi Anik Suryanie Sarudji Susilo, Rahadian Indarto Suwarno Suwarno Syifa'ul Lailiyah Tiara Wandira Hariyanto Tri Lestari Tri Wahyu Suprayogi Tyasningsih, Wiwiek Wahjuni, Retno Sri Warsito, Sunaryo Hadi Wayan Ari wijaya Wibawati, Prima Ayu Wijaya, Achmad Arby Wilujeng, Estiary Wira Tirta Jaladara Yudhana, Aditya Yulianti, Yunika Tri Yumna, Raissa Amelia Yunika Tri Yulianti Yunita, Maya Nurwartanti Zahrudin, Elham