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All Journal AGRITROP Jurnal Ilmu Dasar Melintas An International Journal of Philosophy and Religion Prosiding Seminar Biologi JURNAL TEKNOLOGI LINGKUNGAN AL KAUNIYAH BERKALA SAINSTEK Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Industrial and Systems Engineering Assessment Journal (INASEA) Agrointek Indonesian Journal of Agricultural Science Jurnal AgroBiogen Buletin Penelitian Tanaman Rempah dan Obat ROTOR: JURNAL ILMIAH TEKNIK MESIN UNEJ e-Proceeding Extension Course Filsafat ( ECF ) Agriprima : Journal of Applied Agricultural Sciences Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal ASET (Akuntansi Riset) ANNALES BOGORIENSES Mesin Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Jurnal Pengabdian Magister Pendidikan IPA YUME : Journal of Management ACCRUALS (Accounting Research Journal of Sutaatmadja) J-Dinamika: Jurnal Pengabdian Kepada Masyarakat JURNAL REFORMASI ADMINISTRASI Jurnal Ilmiah untuk Mewujudkan Masyarakat Madani Jurnal Mantik Journal of Contemporary Accounting Jurnal Informasi dan Teknologi Jurnal JTIK (Jurnal Teknologi Informasi dan Komunikasi) JASS (Journal of Accounting for Sustainable Society) Buletin Penelitian Tanaman Rempah dan Obat Economics and Digital Business Review TSARWATICA (Islamic Economic, Accounting, and Management Journal) Operations Research: International Conference Series International Journal of Quantitative Research and Modeling Proceeding Biology Education Conference Golden Ratio of Marketing and Applied Psychology of Business BIOVALENTIA: Biological Research Journal Golden Ratio of Mapping Idea and Literature Format Golden Ratio of Data in Summary Journal of Artificial Intelligence and Digital Business Journal of Law, Education and Business Kajian Akuntansi Jurnal Medika: Medika
Bambang Sugiharto
Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Jember
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AKTIVITAS SUCROSE PHOSPHATE SYNTHASE KULTIVAR TEBU (SACCHARUM OFFICINARUM L.) TOLERAN DAN SENSITIF SELAMA CEKAMAN KEKERINGAN SUCROSE PHOSPHATE SYNTHASE ACTIVITY ON TOLERANCE AND SENSITIVE SUGARCANE CULTIVAR DURING WATER STRESS Rinanto, Yudi; Sugiharto, Bambang
Prosiding Seminar Biologi Vol 8, No 1 (2011): Seminar Nasional VIII Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (436.593 KB)

Abstract

ABSTRAK   Penelitian telah dilaksanakan untuk mengkaji aktivitas enzim SPS (Sucrose Phosphate Synthase) dan ekspresi gen SPS tanaman tebu pada kondisi cekaman kekeringan.  Sebanyak 3 varietas  masing-masing mewakili toleran, moderat dan peka kekeringan ditanam dalam polibag dengan media tanah masing-masing diulang 5 kali.  Setelah berumur 2 bulan diperlakukan cekaman kekeringan dengan cara tanpa diberi pengairan sampai menunjukkan gejala daun menggulung pada daun pertama (K1).  Sampel daun diekstrasi untuk pengujian lebih lanjut. Pengujian enzimatis dilakukan menggunakan sampel daun pertama (K1) tehadap ke 3 varietas masing-masing diulang 3 kali, setelah diperlakukan cekaman kekeringan kemudian dilakukan analisa.  Pengujian enzimatis dilakukakan terhadap enzim SPS yang merupakan enzim penentu yang berperan mengakumulasi sukrosa dalam batang tebu. Hasil penelitian menunjukkan respon aktivitas SPS varietas tebu toleran, moderat dan peka terhadap cekaman kekeringan berbeda-beda.  Aktivitas SPS ke tiga varietas meningkat selama cekaman kekeringan, dengan kecenderungan M442-51 menghasilkan aktivitas lebih tinggi dibanding dua varietas lainnya. Ekspresi gen SPS mengalami peningkatan selama cekaman kekeringan. Perlakuan hormon BA (Benzil Adenin), JA (Jasmonic Acid) dan IAA (Indole Acetic Acid) tidak mampu meningkatkan ekspresi gen SPS, Rubisco maupun GS (Glutamin Synthase).  Cekaman kekeringan dan ABA menyebabkan peningkatkan aktivitas SPS. Berdasarkan hasil penelitian ini diusulkan untuk menggunakan SPS sebagai indikator fisiologis faktor ketahan kekeringan pada tebu.   Kata Kunci : tebu, cekaman kekeringan dan aktivitas SPS
PENELITIAN TINGKAT TOKSISITAS PRODUK KOMPONEN BANGUNAN DARI BAHAN DRILL CUTTING Husin, Andriati Amir; Lasino, Lasino; Sugiharto, Bambang
Jurnal Teknologi Lingkungan Vol. 12 No. 3 (2011)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (347.982 KB) | DOI: 10.29122/jtl.v12i3.1233

Abstract

Sumber daya panas bumi adalah salah satu sumber energi paling potensial di dunia. Dan salah satu produk samping panas bumi aktivitas pengeboran sumur adalah dalambentuk pasir berlumpur. Ini berisi bahan kimia tertentu yang dapat mencemari lingkungan jika tidak ditangani dan direncanakan secara baik. Produk penyebaranPemotonganBor termasuk dalam kategori limbah berbahaya dan beracun berdasarkan Peraturan Pemerintah no 85 (1999). Namu demikian produk sampai tersebut dapat digunakankembali dengan ketentuan bahwa logam berat yang terkandung kontaminan berada pada tingkat yang dapat diterima berdasarkan uji kronis sub. Hal ini karena pemotongan bor tidak aman bagi organisme hidup dan kesehatan manusia. Dalam penelitian ini kami menganalisis kontaminasi logam berat dengan mengekstraksi TCLP dari beberapa komponen bangunan seperti blok beton berongga, genteng beton, paving blok dan panel beton. Pemotongan bor konten yang digunakan adalah 60% untuk blok betonberongga, genteng beton, panel beton dan 40% untuk paving block. Hasil penelitian menunjukkan bahwa kandungan logam berat dalam komponen bangunan yang diuji berada di bawah tingkat batas toleransi dan karena itu dapat diterima.
TRANSFORMASI GENETIK DAN EKSPRESI MUTAN SUCROSE PHOSPHATE SYNTHASE PADA TANAMAN TOMAT Fibriani, Suwinda; Agustien, Inyana Dwi; Sawitri, Widhi Dyah; Sugiharto, Bambang
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (699.256 KB) | DOI: 10.29122/jbbi.v6i1.3341

Abstract

Genetic Transformation and Expression of Sucrose Phosphate Synthase Mutant in Tomato Plant ABSTRACTSucrose phosphate synthase (SPS) is a key enzyme responsible for sucrose biosynthesis. In its regulation, SPS activity is modulated by an allosteric effector glucose-6-phosphate (G6P) suggested to have an ability to bind SPS N-terminus domain. To understand the role of N-terminus in regulating SPS, the SPS gene was mutated with the deletion of N-terminus domain (∆N-SPS). The ∆N-SPS gen was transformed into tomato plants with 5% transformation efficiency. Three transgenic tomato plant 4.20, 5.5.1, and 5.10 were obtained and confirmed by PCR analysis. Transgenic tomato expression was characterized by enzymatic analysis. Result showed that the G6P allosteric regulation in transgenic ∆N-SPS had lost and the SPS activity increased by 2-fold compared to non-transgenic plant. This showed that N-terminus domain-deleted SPS could be actively expressed in plant. Keywords: enzyme, genetic transformation, N-terminus domain deletion, sucrose phosphate synthase, tomato ABSTRAKSucrose phosphate synthase (SPS) merupakan enzim kunci yang bertanggung jawab dalam sintesis sukrosa. Dalam regulasinya, aktifitas SPS dipengaruhi oleh alosterik efektor glukosa-6-fosfat (G6P) yang diduga dapat berikatan pada domain N-terminus SPS. Untuk mengetahui peran N-terminus pada regulasi SPS, dilakukan mutasi SPS dengan penghilangan domain N-terminus (∆N-SPS). Gen ∆N-SPS diinsersi pada tanaman tomat melalui transformasi genetik dengan efisiensi transformasi 5%. Tiga tanaman transgenik tomat (event4.20; 5.5.1; dan 5.10) didapatkan dan positif terkonfirmasi melalui analisis PCR. Ekspresi mutan dikarakterisasi melalui analisis enzimatik. Hasil menunjukkan bahwa tanaman tomat transgenik ∆N-SPS tidak dipengaruhi regulasi alosterik G6P dan aktifitas SPS 2 kali lipat lebih tinggi daripada tanaman bukan transgenik. Ini menunjukkan bahwa SPS dengan delesi domain N-terminus dapat terekspresi aktif pada tanaman.  Kata Kunci: delesi domain N-terminus, enzim, sucrose phosphate synthase, tomat, transformasi genetik 
Pengaruh Locus of Control, Ethical Sensitivity, Kecerdasan Intelektual, Kecerdasan Emosional, Kecerdasan Spiritual dan Tingkat Pendidikan Terhadap Perilaku Etis Astuti, Rika; Sugiharto, Bambang; kurniawan, Asep
Jurnal ASET (Akuntansi Riset) Vol 11, No 2 (2019): Jurnal ASET (Akuntansi Riset) Juli -Desember 2019 [DOAJ & SINTA INDEXED]
Publisher : Universitas Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17509/jaset.v11i2.19921

Abstract

Abstract. The purpose of this study was to determine the effect of locus of control, ethical sensitivity, intellectual intelligence, emotional intelligence, spiritual intelligence and education level on ethical behavior in accounting students of vocational high schools (SMK) and accounting students in Subang. This research is included in research with quantitative data. The sample in this study were 407 respondents consisting of SMK accounting students and accounting students in Subang. The sample selection technique used is probability sampling while for sampling in this study using simple random sampling. The method used for data collection is the survey method through a questionnaire or questionnaire. The data analysis technique used is descriptive statistical analysis and infective statistical analysis. The results showed that locus of control, ethical sensitivity, intellectual intelligence, spiritual intelligence, and education level had a positive and significant effect on the ethical behavior of SMK accounting students and accounting students in Subang. While emotional intelligence does not affect the ethical behavior of SMK accounting students and accounting students in Subang
Pengembangan Modul IPA Materi Tata Surya Berbasis Perspektif Ayat-Ayat Al Qur’an Pada SMPIT dan MTs Sugiharto, Bambang; Cintamulya, Imas
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 15, No 1 (2018): Proceeding Biology Education Conference
Publisher : Universitas Sebelas Maret

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Abstract

This research is a development research with the aim to produce product in the form of IPA Module of Solar Matter Based on Perspective Al-qur'an Verses In SMPIT and MTs class VII. The problems in this study include, the limited module used as a reference of learning that contain the relationship between science materials and verses of the Qur'an. So that raises the formulation of the problem how to develop IPA module Solar Problem Material Based on Perspectives Al-qur'an verses are valid, practical and effective. This research is expected to facilitate the comprehension of students in understanding the science materials related to the verses of Al-Qur'an and not only to increase the knowledge of students but also to play a supporting role in filling the students character education. This research development uses R & D method (Research and Development) with 4-D development model (Four D Model) with three stages of research that is Define (define), Design (design) and Develop (development). The module validation test generated in this study was conducted by 3 expert validators in the field of science education.The trials were conducted on 20 MTs students. Manbail Futuh Jenu class VII. Data collection to test the practicality of this research using observation and test techniques. Collecting data of effectiveness using questionnaire technique. The results show that the modules developed have met the valid, practical and effective criteria.
A SIMPLE PROTOCOL FOR SOMATIC EMBRYOGENESIS INDUCTION OF IN VITRO SUGARCANE ( Saccharum officinarum. L) BY 2,4-D AND BAP Widuri, Laily Ilman; Dewanti, Parawita; Sugiharto, Bambang
BIOVALENTIA: Biological Research Journal Vol. 2 No. 1 (2016)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (288.079 KB) | DOI: 10.24233/BIOV.2.1.2016.32

Abstract

Induction of in vitro sugarcane through somatic embryogenesis technique influenced by addition of plant growth regulator. The objective of this research was to determine appropriate formulation medium for indirect somatic embryogenesis induction on two potential sugarcane SUT Event 02 and PS 881. This research carried out in three steps, callus induction, callus proliferation, and shoot regeneration. Explants taken from basal of in vitro plantlet one month SUT Event 02 and PS 881 resulted from shoot regeneration previously. Five different medium formulas applied for callus induction and one formula for proliferation and shoot regeneration. Research using completely randomized design (CRD) factorial with five different formulation induction mediums. The result showed that the best respond of indirect somatic embryogenesis on SUT Event 02 and PS 881 was medium containing  3 mgL-1of  2,4-D.
TRANSFORMASI GENETIK DAN EKSPRESI MUTAN SUCROSE PHOSPHATE SYNTHASE PADA TANAMAN TOMAT Suwinda Fibriani; Inyana Dwi Agustien; Widhi Dyah Sawitri; Bambang Sugiharto
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (699.256 KB) | DOI: 10.29122/jbbi.v6i1.3341

Abstract

Genetic Transformation and Expression of Sucrose Phosphate Synthase Mutant in Tomato Plant ABSTRACTSucrose phosphate synthase (SPS) is a key enzyme responsible for sucrose biosynthesis. In its regulation, SPS activity is modulated by an allosteric effector glucose-6-phosphate (G6P) suggested to have an ability to bind SPS N-terminus domain. To understand the role of N-terminus in regulating SPS, the SPS gene was mutated with the deletion of N-terminus domain (∆N-SPS). The ∆N-SPS gen was transformed into tomato plants with 5% transformation efficiency. Three transgenic tomato plant 4.20, 5.5.1, and 5.10 were obtained and confirmed by PCR analysis. Transgenic tomato expression was characterized by enzymatic analysis. Result showed that the G6P allosteric regulation in transgenic ∆N-SPS had lost and the SPS activity increased by 2-fold compared to non-transgenic plant. This showed that N-terminus domain-deleted SPS could be actively expressed in plant. Keywords: enzyme, genetic transformation, N-terminus domain deletion, sucrose phosphate synthase, tomato ABSTRAKSucrose phosphate synthase (SPS) merupakan enzim kunci yang bertanggung jawab dalam sintesis sukrosa. Dalam regulasinya, aktifitas SPS dipengaruhi oleh alosterik efektor glukosa-6-fosfat (G6P) yang diduga dapat berikatan pada domain N-terminus SPS. Untuk mengetahui peran N-terminus pada regulasi SPS, dilakukan mutasi SPS dengan penghilangan domain N-terminus (∆N-SPS). Gen ∆N-SPS diinsersi pada tanaman tomat melalui transformasi genetik dengan efisiensi transformasi 5%. Tiga tanaman transgenik tomat (event4.20; 5.5.1; dan 5.10) didapatkan dan positif terkonfirmasi melalui analisis PCR. Ekspresi mutan dikarakterisasi melalui analisis enzimatik. Hasil menunjukkan bahwa tanaman tomat transgenik ∆N-SPS tidak dipengaruhi regulasi alosterik G6P dan aktifitas SPS 2 kali lipat lebih tinggi daripada tanaman bukan transgenik. Ini menunjukkan bahwa SPS dengan delesi domain N-terminus dapat terekspresi aktif pada tanaman.  Kata Kunci: delesi domain N-terminus, enzim, sucrose phosphate synthase, tomat, transformasi genetik 
KEAMANAN PANGAN KOMODITAS TRANSGENIK: STUDI KASUS SEDIAAN PAKAN HIJAUAN DARI DAUN TEBU OVEREKSPRESI SoSPS1 A Bagus Nur Sudrajat; Nurhayati Nurhayati; Bambang Sugiharto
AGROINTEK Vol 15, No 3 (2021)
Publisher : Agroindustrial Technology, University of Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrointek.v15i3.7868

Abstract

Sugarcane (Saccharum officinarum L) is a sucrose-producing plantation that has been cultivated in various countries. Genetically modified sugar cane sugar cane plants are sugar cane plants that are assembled biotechnology through overexpression of the SoSPS1 gene. The SoSPS1 gene is responsible for the biosynthesis of sucrose sugar in leaves. Waste in the form of leaves, shoots, and bagasse has not been used optimally as animal feed. Thus it takes a lot of innovation and appropriate technology in the use of sugarcane waste for animal feed, so that it is expected to achieve zero waste farming systems that can be utilized all without being wasted and polluting the environment. As an alternative feed for livestock, then genetically engineered sugar cane must be tested for feed safety. Feed safety testing includes substantial equivalence value and feed digestibility test in which genetically engineered products have nutritional and digestive values commensurate with conventional products. Substantial value of sugarcane planted by genetically engineered overexpression of SoSPS1 gene did not differ significantly compared to conventional sugarcane. However, there are some significant nutritional content, but these values are still in the range of values in the OECD.
DNA Barcoding of Medicinal Orchid Dendrobium discolor Lindl. Tanimbar Using rbcL and ITS genes Dian Al Ghifari Perwitasari; Siti Rohimah; Tri Ratnasari; Bambang Sugiharto; Mukhamad Su'udi
Buletin Penelitian Tanaman Rempah dan Obat Vol 31, No 1 (2020): Buletin Penelitian Tanaman Rempah dan Obat
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v31n1.2020.8-20

Abstract

Dendrobium discolor Lindl., Tanimbar is one of the medicinal orchids that has been used to treat skin diseases. Morphologically, D. discolor Tanimbar shows similarities with D. discolor Merauke and D. bigibbum, making it challenging to identify. DNA barcoding using ribulose-1,5-bisphosphate carboxylase large (rbcL) and Internal Transcribed Spacer (ITS) markers expected to be used to identify D. discolor accurately. This study aimed to identify potential DNA sequences as barcodes for the identification of medicinal orchid D. discolor Tanimbar using molecular markers rbcL and ITS. The DNA genome of D. discolor Tanimbar was isolated and used as a template in the PCR reaction. The resulting amplicons were then sequenced. The results showed that the rbcL gene sequence of D. discolor had high homology with D. salaccense (Accession: LC193510.1, Prect. Ident  :   99.45 %),   whereas   the   ITS   had  high homology with D. nindii (Accession: AY239985.1 Identification: 98.67 %). Bioinformatics analysis showed that the rbcL gene sequence from D. discolor had more homology sequence than the ITS. However, the ITS sequence was more specific and could differentiate to species level. Based on the results of this study, the ITS sequence can be recommended as a molecular marker for the identification of the medicinal orchid D. discolor Tanimbar.
Isolasi dan Identifikasi Khamir Toleran Alkohol dari Molase Nurhayati; Jay Jayus; Anjas Wida Elistia Rini; Bambang Sugiharto; Dedy Eko Rahmanto
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 7, No 1 (2022): February 2022
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v7i1.5426

Abstract

Khamir mampu menghasilkan bioetanol dari molases. Selama proses produksi (proses fermentasi) terjadi proses akumulasi alkohol sehingga konsentrasi alkohol semakin meningkat. Konsentrasi etanol yang melebihi 8% (v/v) dapat menyebabkan ketidakstabilan membran yang kemudian dapat menurunkan permeabilitas membran dan homeostasis sel. Penelitian ini dilakukan isolasi dan identifikasi khamir indigenus dari molase yang toleran terhadap alkohol. Karakterisasi morfologi meliputi bentuk koloni, bentuk sel dan tipe pertunasa, sedangkan karaktersasi fisiologis dilakukan dengan kit API 20C. Identifikasi molekuler dilakukan dengan PCR dan sequencing DNA menggunakan primer ITS1-ITS4. Hasil penelitian didapatkan dua isolat khamir yaitu isolat X dan Z yang memiliki potensi toleran terhadap alkohol. Berdasarkan hasil karakteristik fisiologis, isolat X dan isolate Z memiliki kedekatan dengan Candida tropicalis. Hasil sequencing DNA dengan primer ITS1-ITS4 dan analisis dengan program blastn menunjukkan bahwa isolat X teridentifikasi sebagai spesies Candida parapsilosis strain AUMC 10714 dan isolat Z teridentifikasi sebagai spesies Candida parapsilosis ZA012.
Co-Authors A Bagus Nur Sudrajat A. Dinar Ambarwati Agung Nur Probohudono Agustien, Inyana Dwi Agustien, Inyana Dwi Ahmad Hidayat Ahmad Ilham Tanzil Ai Sumirah Setiawati Amir, Muhammad Hisyam Andriani, Maya Anjas Wida Elistia Rini Anuary Dwi Rosyid, Wafa Prasetia Arif Syaifudin, Arif Ariyono, Alief Rizky Asshidiqie, Wahyu Pratama Azizah, Kunti Anis Bambang Sugiharto Bayati, Tutiana Bon, Abdul Thalib Bin Budi Sulistiarini, Emma Dedi Dwilaksana Dedy Eko Rahmanto Dewita, Tiara Ajeng Dian Al Ghifari Perwitasari Digdo Listyadi, Digdo Djenal . Dr. Hadrianus Tedjoworo, S.Ag., STL. Dwi Djumhariyanto Edia F.D Edia, Edia F.D Edison Edison Edy Listanto Elizabeth Eneng Tita Tosida Eny I. Riyanti Erlia Narulita Esti Utarti Fajrianto, Sigit Fanata, Wahyu Indra Duwi Fibriani, Suwinda Gaol, Nickytha Linch De Hans Lumban Gulo, Desi Natalia Hafiz, Syauqi Abyan Hafizi, Muhamad Halawa, Herry Martinus Hari Purnomo Harkim, H. Harkim, Harkim Hasibuan, Aqil Muhammad Hayati, Nur Fadillah Hiya, Nirmadarningsih Husin, Andriati Amir Icih, Icih Ika Oktavianawati Ilhamsyah Siregar Imas Cintamulya Insani, Vivian Nurul Intan Ria Neliana Inyana Dwi Agustien Irawan Gulo, Sastra Darma Iryani Endah Febrianti Jayus Jayus Kesuma, Muhammad Yudha Kurnia Indriyanti Purnamasari, Kurnia Indriyanti Kurniawan , Asep Kusmana Kusmana Laily Ilman Widuri, Laily Ilman Laksono Trisnantoro Lasino Lasino, Lasino Lassa, Anita Lathifah Hanum, Lathifah Liem, Jhon Sepron Defretus Lina Maulidiana Maesaroh, Nurlaila Maisaro, Maisaro Marina Pakaya Maulani, Ardela Alief MAYA ANDRIANI Media Ningtyas, Rinda MISWAR - Mochamad Asrofi Mohd Radzi, Fathin Nuril A'isha Binti Muhammad Hazmi Muhammad, Adibillah Mukhamad Su'udi Nadya Oktafiana Neliana, Intan Ria Netty Ermawati Nikmah, Nasilatun Nina Oktaria, Nina Ningtiyas, Wulan Nursyiam Nirwana Br Bangun Nugroho, Sandi Luqman Nurfatimah, Siti Nurhayati Nursely, Dinda Oktapan, Ladefa Primana Parawita Dewanti Purnama Okviandari, Purnama Purwinto, Rahmanaji Setyo Putri, Trisandi Eka Rachman Setiawan Rahardhianto, Arsetyo Raharja, Ajung Gilang Putra Raharjo, Sulistyo Yusuf Rahma Rei Sakura Ramadhan, Mohamad Ibnu Ramadhani, Rifki Fahrisal Ratnasari, Tri Ratri Ciptaningtyas Rejekia V Simanungkalit Restanto, Didik Pudji Rizal, Asep Rizki, Ainur Salahuddin Junus Salih, Yasir Sigit Soepardjono Simamora, Harkim Simanungkalit, Rejekia Vaizal Sirait, Syah Paradiba Siti Arifah Siti Rohimah Siti Umayyah Soleh, Mohamad Badrus SRI AYU MADE Sri Mulyati Sri Mulyati Sri Panca Setyawati Sri Rejeki Subandi, Kotim Sugara, Victor Ilyas Sugianto, Efendi Sukardiman Syafina Pusparani Tanjung, Yulia Tiara Toto Hadiarto Tri Agus Siswoyo TRI HANDOYO Tri J. Santoso Tri Ratnasari Ummi Sholikhah Untung Murdiyanto Wahyu Nurkholis Hadi Syahputra Widhi Dyah Sawitri Widhiya Ninsiana, Widhiya Wulan Nursyiam Ningtyas Yanti Setianti Yayuk Fatmawati Yoga Yuniadi Yudi Rinanto YULIANINGSIH, LILIK Yundari, Yundari Yusuf Rachmandika