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All Journal Vegetalika AGRITROP International Journal of Biosciences and Biotechnology Jurnal Ilmu Dasar JURNAL TEKNOLOGI LINGKUNGAN AL KAUNIYAH BERKALA SAINSTEK Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Agrointek Indonesian Journal of Agricultural Science Jurnal AgroBiogen Buletin Penelitian Tanaman Rempah dan Obat ROTOR: JURNAL ILMIAH TEKNIK MESIN Indonesian Journal of Biotechnology UNEJ e-Proceeding Agriprima : Journal of Applied Agricultural Sciences Jurnal Bioteknologi & Biosains Indonesia (JBBI) ANNALES BOGORIENSES Mesin Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Jurnal Pengabdian Magister Pendidikan IPA J-Dinamika: Jurnal Pengabdian Kepada Masyarakat Jurnal Informasi dan Teknologi Jurnal JTIK (Jurnal Teknologi Informasi dan Komunikasi) Buletin Penelitian Tanaman Rempah dan Obat International Journal of Quantitative Research and Modeling BIOVALENTIA: Biological Research Journal Journal of Law, Education and Business Biosaintifika: Journal of Biology & Biology Education

Bambang Sugiharto

Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Jember

Author-ID : 388822

Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Social Sciences

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Budidaya Terpadu Ikan dan Sayuran melalui Metode Akuaponik Di Desa Serut Kecamatan Panti Kabupaten Jember Parawita Dewanti; Sigit Soepardjono; Didik Pudji Restanto; Bambang Sugiharto
J-Dinamika : Jurnal Pengabdian Masyarakat Vol 5 No 1 (2020): Juni
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/j-dinamika.v5i1.1398

Sosialisasi dan praktek usaha hidroponik sudah dilakukan melalui posdaya yang dibentuk oleh Lembaga Pengabdian Universitas Jember di Dusun Karang Anom desa Serut Kecamatan Panti Kabupaten Jember. Desa Serut dipilih sebagai target pada program pengembangan desa mitra karena mempunyai potensi tinggi untuk pengembangan sayuran hidroponik ditinjau dari lokasi, kepedulian dan kemandirian masyarakatnya serta mempunyai jiwa kewirausahaan. Disisi lain, hasil observasi ditemukan pula sebagian penduduk di Dusun Karang Anom desa Serut Kecamatan Panti memiliki usaha budidaya ikan yang sudah berjalan beberapa tahun terakhir. Upaya Budidaya terpadu sayuran dan ikan melalui metode Akuaponik diharapkan menghasilkan 2 produk sekaligus dalam satu kali proses produksi, sehingga akan diperoleh produk yang bermanfaat bagi masyarakat setempat dan masyarakat secara luas untuk penenuhan pangan dan gizi keluarga.Target yang diharapkan adalah agar anggota kelompok mitra memiliki kemampuan dan ketrampilan dalam mengelola budidaya terpadu ikan dan sayuran dalam satu kali proses produksi. Oleh karena itu diperlukan sarana dan prasarana yang memadai sehingga kelompok mitra dapat memanfaatkan teknologi akuaponik. Hasil kegiatan menunjukkan bahwa terjadi respon positif dari anggota kelompok mitra yang ditunjukkan oleh antusias peserta dalam mengikuti serangkaian pelatihan dari awal sampai selesai. Telah diperoleh hasil produk dalam satu kali produksi akuaponik per bulan akan menghasilkan ikan lele sebanyak 50 kg dan sayuran 40 kg. Untuk 3 unit kolam ikan akan dihasilkan ikan lele 150 kg dan 120 kg sayuran dalam waktu 3 bulan

Produksi Antibodi Poliklonal Menggunakan Protein Rekombinan RBD-spike Untuk Deteksi SARS-CoV-2 Iryani Endah Febrianti; Yayuk Fatmawati; Intan Ria Neliana; Widhi Dyah Sawitri; Erlia Narulita; Bambang Sugiharto
Al-Kauniyah: Jurnal Biologi Vol 16, No 2 (2023): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v16i2.25664

AbstrakSARS-CoV-2 merupakan virus yang menyebabkan Coronavirus Disease 2019 (COVID-19) di seluruh dunia dan sampai saat ini kasus terbaru masih terus dilaporkan. Diagnostic test merupakan hal yang krusial untuk dikembangkan. Prinsip diagnostic test COVID-19 berbasis antigen, yaitu mendeteksi virus SARS-CoV-2 melalui respon antibodi dari penderita. Penelitian ini bertujuan untuk memproduksi antibodi poliklonal menggunakan protein rekombinan RBD-Spike untuk mendeteksi virus SARS-CoV-2 berbasis antibodi. Penelitian dimulai dengan penentuan domain RBD-Spike menggunakan pensejajaran asam amino, dan konstruksi DNA untuk RBD-Spike pada vektor ekspresi pET28a menggunakan sintetik nukleotida. Produksi protein rekombinan RBD-Spike diekspresikan pada sel bakteri Escherichia coli. Purifikasi dilakukan untuk memperoleh protein RBD-Spike dan selanjutnya digunakan sebagai antigen untuk induksi antibodi poliklonal pada kelinci. Hasil penelitian menunjukkan bahwa ekspresi protein rekombinan RBD-Spike SARS-CoV-2 memerlukan induksi IPTG 0,1 mM dan terekspresi dalam bentuk inclusion bodies dengan ukuran 39 kDa. Purifikasi protein RBD-Spike dilakukan menggunaan resin afinitas NiNTA, elektroelusi, dan dialisis. Total protein RBD-Spike yang diperoleh sebanyak 4 mL dengan konsentrasi 10 mg/mL. Analisa Ouchterlony menunjukkan bahwa antibodi poliklonal terdeteksi pada minggu kedua setelah injeksi booster dan analisa spesifitas antibodi terhadap antigen menunjukkan bahwa antibodi poliklonal dapat mendeteksi protein RBD-Spike pada konsentrasi 0,1 µg. Selanjutnya diharapkan antibodi poliklonal dapat digunakan untuk deteksi keberadaan virus SARS-CoV-2 dan dapat dikembangkan untuk kit deteksi berbasis antibodi.AbstractSARS-CoV-2 is the virus that causes Coronavirus Disease 2019 (COVID-19) worldwide and the latest cases are still being reported until now. The diagnostic test is a crucial to be developed. The principle of the antigen-based COVID-19 diagnostic test is to detect the SARS-CoV-2 virus through antibody response from the patients. This study was conducted to produce polyclonal antibodies using recombinant protein RBD-Spike. The research was carried out by determining the RBD-Spike domain using amino acid alignment and constructing the DNA of RBD-Spike to the expression vector of pET28a using nucleotide synthesis. Production of RBD-Spike recombinant protein was expressed in Escherichia coli. Purification was carried out to obtain RBD-Spike protein and used to induce polyclonal antibody ina rabbit. The results showed that the expression of RBD-Spike recombinant protein required induction of IPTG 0.1 mM and was expressed in inclusion bodies with molecular size of 39 kDa. The purification of RBD-Spike protein was carried out using resin affinity, electroelution, and dialysis. The total protein of RBD-Spike obtained was 4 mL with a concentration of 10 mg/mL. Ouchterlony analysis revealed that polyclonal antibody was detected in the second week after booster injection and analysis of antibody specificity showed that polyclonal antibodies detected RBD-Spike protein at the concentration of 0.1µg of RBD-Spike protein. Moreover, it is expected that our polyclonal antibody detect the SARS-CoV-2 virus and can be developed for antibody-based detection kits.

Pemanfaatan Data Analytics untuk Mengkaji Pengaruh Motivasi, Kepuasan Kerja, dan Lingkungan Terhadap Kinerja Karyawan di Glory Industrial Semarang Rejeki, Sri; Sugianto, Efendi; Sugiharto, Bambang; Lassa, Anita; Liem, Jhon Sepron Defretus
Jurnal JTIK (Jurnal Teknologi Informasi dan Komunikasi) Vol 8 No 4 (2024): OCTOBER-DECEMBER 2024
Publisher : Lembaga Otonom Lembaga Informasi dan Riset Indonesia (KITA INFO dan RISET)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35870/jtik.v8i4.2921

This study employs data analytics to examine the influence of motivation, job satisfaction, and the work environment on employee performance at Glory Industrial Semarang. Using a quantitative approach, the study involved a population of 300 employees, with a sample selected through probability sampling using the simple random sampling technique, resulting in 78 respondents. Data analysis was conducted using SPSS, revealing that the variables of job satisfaction, motivation, and the work environment each have a positive and significant impact on employee performance. The job satisfaction variable has a regression coefficient of 0.293 with a significance of 0.024, the motivation variable has a coefficient of 0.349 with a significance of 0.002, and the work environment variable has a coefficient of 0.262 with a significance of 0.000. These findings confirm that data analytics is effective in identifying factors that contribute to the enhancement of employee performance within the company

Meningkatkan Kesejahteraan Petani: Menuju Sektor Pertanian yang Tangguh dan Berdaya Saing di Indonesia Hasibuan, Aqil Muhammad; Sugiharto, Bambang; Hayati, Nur Fadillah; Dewita, Tiara Ajeng; Bayati, Tutiana
Journal of Law, Education and Business Vol 2, No 2 (2024): Oktober 2024
Publisher : CV. Rayyan Dwi Bharata

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57235/jleb.v2i2.3130

Sektor pertanian memiliki peran vital dalam perekonomian global, terutama di negara berkembang seperti Indonesia. Sektor ini tidak hanya memenuhi kebutuhan pangan nasional tetapi juga menjadi tulang punggung bagi penduduk pedesaan, memberikan kontribusi signifikan terhadap Produk Domestik Bruto (PDB), penciptaan lapangan kerja, dan stabilitas sosial. Penelitian ini mengeksplorasi strategi pengembangan sektor pertanian di Indonesia untuk meningkatkan produktivitas, keberlanjutan, dan kesejahteraan petani melalui analisis kualitatif dari artikel ilmiah serta data dari Badan Pusat Statistik (BPS). Hasil penelitian menunjukkan upaya pemerintah dalam meningkatkan produktivitas melalui teknologi modern, penyediaan kredit, dan pengembangan infrastruktur serta adaptasi terhadap perubahan iklim dan perlindungan lahan. Implementasi regulasi seperti Undang-Undang Pangan dan Peraturan Menteri Pertanian menunjukkan komitmen pemerintah dalam mendukung sektor pertanian.

EFFECT OF VOLUME FRACTION OF GENETICALLY MODIFIED ORGANISM (GMO) SUGARCANE BAGASSE FIBER ON THE MECHANICAL PROPERTIES AND MORPHOLOGY OF BIOCOMPOSITE Anuary Dwi Rosyid, Wafa Prasetia; Sakura, Rahma Rei; Djumhariyanto, Dwi; Asrofi, Mochamad; Setyawan, Digdo Listyadi; Sugiharto, Bambang; Junus, Salahuddin; Dwilaksana, Dedi
ROTOR Vol 16 No 2 (2023)
Publisher : Jurusan Teknik Mesin Fakultas Teknik Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/rotor.v16i2.41551

Science and technology are currently developing in all fields, such as vehicle construction, building construction, industry, and materials engineering. Especially in the field of material engineering used for the manufacture of composite materials. Currently the transition of materials from metal to composites is also in great demand in various fields including in the automotive, transportation, shipping, aerospace, health, and various other fields. In the components of a vehicle, the components that have the most potential to be reduced are the main structural components, namely the floor, body, and ribs. Efforts to reduce the weight of the material in these components are carried out to reduce the amount of fuel consumption, so the ratio of strength and density of a material is one of the important properties that needs to be considered. This study used the hand lay up method with a comparison of volume fraction variations of 15%: 85% and 20%: 80%. With the aim of adding volume fractions with optimal combinations, it is hoped that the tensile strength will get better. The highest average tensile strength is with a volume fraction ratio of 15%: 85% of 19.04 MPa. While the lowest average tensile strength is with a volume fraction ratio of 20%: 80% of 17.78 MPa. It can be concluded that with increasing fiber volume fraction, the tensile strength decreases. This happens because there are several defects in the form of holes and voids which indicate poor bonding between the fibers and the matrix. Keywords: PRG sugarcane fibers, volume fraction, epoxy, tensile properties

Analysis of The Influence of Trust In Digital Transactions and Website Reputation on Customers Purchase Intention Through Footwear Website Setianti, Yanti; Budi Sulistiarini, Emma; Sugiharto, Bambang; Elizabeth; Syaifudin, Arif
Jurnal Informasi dan Teknologi 2024, Vol. 6, No. 1
Publisher : SEULANGA SYSTEM PUBLISHER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.60083/jidt.v6i1.518

This study examines how attitudes regarding online buying and the consequences for purchase intention are influenced by perceived website repute, relative advantage, perceived website image, and trust. Researchers employed a survey method in this study to conduct descriptive research. A questionnaire was used in this survey approach. E-commerce consumers who reside in Jabodetabek make up the study's population. Using judgmental sampling, the researcher employed a non-probability sampling technique. In this study, the single-cross-sectional approach was employed for data collecting. The structural equation model method will be used to analyze the data. Amos is the program that's being used. According to the data processing results, it was discovered that, in the first place, relative profits, trust, and website image are all significantly positively impacted by perceived website reputation. Second, trust, the perception of websites, and attitudes toward online commerce are considerably improved by relative advantage. Third, perceptions of websites have a detrimental effect on attitudes toward internet buying. Fourth, sentiments toward internet purchasing are positively impacted by trust, though not much. Opinions regarding internet purchasing significantly positively influence fifth intentions to purchase. This demonstrates that consumers' intentions to buy through a website positively correlate with their attitude about online buying.

Effect of Drought Stress on Morphology and Chlorophyll Content of Sorghum (Sorghum bicolor L. Moench) Rahardhianto, Arsetyo; Raharjo, Sulistyo Yusuf; Nugroho, Sandi Luqman; Purwinto, Rahmanaji Setyo; Soleh, Mohamad Badrus; Sugiharto, Bambang
Jurnal ILMU DASAR Vol 26 No 1 (2025)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v26i1.32122

Sorghum is a type of cereal plant that easily adapts to stressful environmental conditions. Sorghum has many benefits as it can be used as food, feed, and energy sources, making it a promising crop for development on dry land in Indonesia. Drought impacts plants during their growth process, causing physiological and biological changes. This study aims to determine the effect of drought stress on the morphology and chlorophyll content of sorghum plants. Preliminary research was conducted by planting three varieties of sorghum (Numbu, Super 1, and Super 2). Subsequent planting involved selecting the variety most resistant to drought based on data analysis of several parameters. The basic design used in this experiment was a completely randomized design (CRD) with one factor: the level of drought stress, which had four levels -no drought stress as a day 0 (control) and drought stress for 3, 6 and 9 days by withholding water. The results showed a decrease in the total chlorophyll content of the leaves over time. Drought stress for 6 and 9 days without watering resulted in water content dropping below 25%, indicating severe drought stress and disrupting plant growth. Severe drought stress was characterized by low soil and leaf water content, yellowing and curling of the leaves.

The Effect of Concentration and Exposure Time Acyclovir for Elimination Sugarcane Mosaic Virus (SCMV) on The Apical Bud Culture of Sugarcane PS 881 Maisaro, Maisaro; Sugiharto, Bambang; Dewanti, Parawita
Jurnal ILMU DASAR Vol 18 No 1 (2017)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

SCMV (Sugarcane Mosaik Virus) is sugarcane crop disease resulting in chlorosis in leaves with the formation of the colors yellow and green intermittents. Based on survey information obtained that the air observation of all varieties of sugarcane was already stricken with the virus SCMV. Even the most formidable attack is on PS881 varieties with the intensity of the attacks reached 80%, so that it is estimated will lose up to 40% of the harvest. The sugarcane is virus free can be obtained via organogenesis in tissue culture method directly on the apical meristem, somatic embryogenesis at, and also with the addition of khemoterapeutan (acyclovir).The workings of the khemoterapi materials are the chemotherapi will interfere with replication and synthesis of genetic material of the virus but also cause the same effect against the mechanism of synthesis of nuklet acid on plants hosts. This research aims to find the best concentration and exposure time the most good in eliminating viruses SCMV in the apical bud culture of sugarcane PS881, using the antiviral acyclovir in conditions of invitro, so that the resulting plant will be virus free. The methods used to detect the presence of the virus by using the two ways, the first is by serology test through the protein content of the virus / checking nucleic acid virus with ELISA and the second is by RT-PCR. The results of the analysis showed that the interaction between the concentration of chemotherapy and exposure time produces the best treatment in eliminating the virus was . treatment with acyclovirc oncentration of 20 ppm and exposure time of 5 weeks.

Construction and expression of Wee1 recombinant protein in Escherichia coli strain BL21 (DE3) Oktapan, Ladefa Primana; Maulani, Ardela Alief; Ermawati, Netty; Sugiharto, Bambang
Jurnal ILMU DASAR Vol 24 No 2 (2023)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v24i2.28232

Wee1 is a gene encoding for protein kinase that is located in the nucleus and it plays an essential role in determining the timing of mitosis. Overexpression of Wee1 in rice is resulting in increased plant size. However, the increasing plant size due to the increase of Wee1 protein expression is not elucidated. Immunodiagnostic using specific antibodies against Wee1 protein should be conducted to determine the role of the protein on cell division. This experiment was directed to construct Wee1 in an expression vector for synthesis of recombinant Wee1 protein using three strategies of construction. The three strategies were construction of full length (FL), deletion of putative binding site (BS), and deletion of N-terminal domain (ΔN) of Wee1. Restriction analysis with BamH1/Sal1 of FL-Wee1, BS-Wee1, and ΔN-Wee1 construct resulted in the different DNA fragments with molecular size at 1239, 1176, and 960bp, respectively. This result indicated that the Wee1 fragments have been successfully inserted in the expression plasmid which was further confirmed using DNA sequencing. Colony PCR analysis showed that Escherichia coli strain BL21 (DE3) has been transformed with the constructs. The protein analysis using SDS-PAGE revealed that the recombinant protein of Wee1 synthesized in the E.coli containing ΔN-Wee1 construct, but not in FL-Wee1 and BS-Wee1 constructs. The ΔN-Wee1 protein was synthesized in an insoluble fraction with a molecular size of 38.8 kDa which is the same as the size estimated using the software ExPASy. Interestingly, the level of synthesized ΔN-Wee1 protein was not induced by IPTG concentration. Collectively, the results indicated that the DNA construct of ΔN-Wee1 is suitable for recombinant protein production.

Enhancement of Regeneration Efficiency through Callus Induction Media Using 2,4-Dicholorophenoxyacetic acid in Indica Rice (Oryza sativa L. var. Ciherang) Azizah, Kunti Anis; Restanto, Didik Pudji; Sugiharto, Bambang
Jurnal ILMU DASAR Vol 18 No 2 (2017)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Indica rice variety Ciherang is the most planted variety in Indonesia, but the micropropagation technique is restricted because it is known has low regeneration frequency and included as recalcitrant cultivar for tissue culture and tranformation activities. One of solution to resolve that problem is developing a technique of somatic embryogenesis in callus of ciherang rice cultivar. The aims of study were to determine medium composition for inducting embryogenic callus in Ciherang rice and to know the effectivity of rice regeneration using callus as explant. The methods were included induction of embryogenic callus in callus induction media (CIM) containing MS basal, Proline 600 mg/l, Casein Hidrolisat 300 mg/l, phytagel 2,5 g/l, BAP 0.25 mg/l, sukrosa 30 %, and 2,4-D in different concentration, from 2,4-D 0 mg/l as control (CIM 1), 2,4-D 2 mg/l (CIM 2), 2,4-D 3 mg/l (CIM 3), and 2,4-D 4 mg/l (CIM 4). It then be continued to regenerate the calli in RM 1 medium containing MS basal, NAA 0,2 mg/l, Kinetin 2 mg/l, Agarose 10 g/l, and sukrosa 30 %, pH 5,8 for six days in dark and RM2 medium containing MS basal , NAA 0,2 mg/l, Kinetin 2 mg/l, Agarose 8 g/l, sukrosa 30 %, pH 5,8 in light room. Results showed CIM 4 medium using 2,4-D 4 mg/l gave optimum result in calli induction with procentage 57,63% and CIM 3 using 2,4-D 3 mg/l gave optimum result in embryonic calli induction with procentage 53,63%. Result of embryonic calli gave regeneration frequency procentage is 9,6%. The total planlet obtained after five weeks old in regeneration medum were 11 planlets ready for aclimatization. Keywords: Somatic embryo, Indica rice (Oryza sativa L. var. Ciherang), Callus

Co-Authors A Bagus Nur Sudrajat A. Dinar Ambarwati Agustien, Inyana Dwi Agustien, Inyana Dwi Ahmad Ilham Tanzil Amir, Muhammad Hisyam Anjas Wida Elistia Rini Anuary Dwi Rosyid, Wafa Prasetia Arif Syaifudin, Arif Ariyono, Alief Rizky Arsy Chairiyah Asshidiqie, Wahyu Pratama Azizah, Kunti Anis Bayati, Tutiana Bon, Abdul Thalib Bin Budi Sulistiarini, Emma Dedi Dwilaksana Dedy Eko Rahmanto Dewita, Tiara Ajeng Dian Al Ghifari Perwitasari Digdo Listyadi, Digdo Djenal . Dwi Djumhariyanto Edia F.D Edia, Edia F.D Edy Listanto Elizabeth Eneng Tita Tosida Eny I. Riyanti Erlia Narulita Esti Utarti Fajrianto, Sigit Fanata, Wahyu Indra Duwi Ferdi Hariyanto Fibriani, Suwinda Hafiz, Syauqi Abyan Hafizi, Muhamad HARDIAN SUSILO ADDY Hari Purnomo Hasibuan, Aqil Muhammad Hayati, Nur Fadillah Husin, Andriati Amir Ida Ayu Putri Darmawanti Ika Oktavianawati Imas Cintamulya Inyana Dwi Agustien Iryani Endah Febrianti Jayus Jayus Khiar Ayatina Hasbi Kusmana Kusmana Laily Ilman Widuri, Laily Ilman Laksono Trisnantoro Lasino Lasino, Lasino Lassa, Anita Liem, Jhon Sepron Defretus Magnon, Cassidy Maisaro, Maisaro Maulani, Ardela Alief Maulida, Dinda Mala Media Ningtyas, Rinda MISWAR - Mochamad Asrofi Muhammad Hazmi Muhammad, Adibillah Mukhamad Su'udi Muslimah Arniyanti Nadya Oktafiana Neliana, Intan Ria Netty Ermawati Netty Ermawati Ni Putu Ayu Erninda Oktaviani Suputri Nikmah, Nasilatun Nina Oktaria, Nina Ningtiyas, Wulan Nursyiam Nugroho, Sandi Luqman Nurhayati Oktapan, Ladefa Primana Parawita Dewanti Purnama Okviandari Purnama Okviandari, Purnama Purwinto, Rahmanaji Setyo Rachman Setiawan Rahardhianto, Arsetyo Raharja, Ajung Gilang Putra Raharjo, Sulistyo Yusuf Rahma Rei Sakura Ramadhani, Rifki Fahrisal Ratnasari, Tri Restanto, Didik Pudji Retnosari Apriasti Rindang Dwiyani Risky Mulana Anur Salahuddin Junus Salih, Yasir Sigit Soepardjono Siswahyudianto Siti Rohimah Siti Umayyah Soleh, Mohamad Badrus SRI AYU MADE Sri Rejeki Subandi, Kotim Sugara, Victor Ilyas Sugianto, Efendi Sukardiman Syafina Pusparani Toto Hadiarto Tri Agus Siswoyo TRI HANDOYO Tri J. Santoso Tri Ratnasari Ummi Sholikhah Untung Murdiyanto Wahyu Indra Duwi Fanata Wahyu Nurkholis Hadi Syahputra Weny Nailul Hidayati Widhi Dyah Sawitri Wulan Nursyiam Ningtyas Yanti Setianti Yayuk Fatmawati Yoga Yuniadi Yudi Rinanto Yundari, Yundari Yusuf Rachmandika

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