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All Journal AGRITROP Jurnal Ilmu Dasar Melintas An International Journal of Philosophy and Religion Prosiding Seminar Biologi JURNAL TEKNOLOGI LINGKUNGAN AL KAUNIYAH BERKALA SAINSTEK Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Industrial and Systems Engineering Assessment Journal (INASEA) Agrointek Indonesian Journal of Agricultural Science Jurnal AgroBiogen Buletin Penelitian Tanaman Rempah dan Obat AGRIVITA, Journal of Agricultural Science ROTOR: JURNAL ILMIAH TEKNIK MESIN UNEJ e-Proceeding Extension Course Filsafat ( ECF ) Agriprima : Journal of Applied Agricultural Sciences Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal ASET (Akuntansi Riset) ANNALES BOGORIENSES Mesin Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Jurnal Pengabdian Magister Pendidikan IPA YUME : Journal of Management ACCRUALS (Accounting Research Journal of Sutaatmadja) Jurnal Permukiman J-Dinamika: Jurnal Pengabdian Kepada Masyarakat JURNAL REFORMASI ADMINISTRASI Jurnal Ilmiah untuk Mewujudkan Masyarakat Madani Journal Of Management Science (JMAS) Jurnal Review Pendidikan dan Pengajaran (JRPP) Jurnal Mantik Journal of Contemporary Accounting Jurnal Informasi dan Teknologi Jurnal JTIK (Jurnal Teknologi Informasi dan Komunikasi) JASS (Journal of Accounting for Sustainable Society) Buletin Penelitian Tanaman Rempah dan Obat Economics and Digital Business Review TSARWATICA (Islamic Economic, Accounting, and Management Journal) Operations Research: International Conference Series Jurnal Pengabdian Masyarakat (ABDIRA) International Journal of Quantitative Research and Modeling Proceeding Biology Education Conference Golden Ratio of Marketing and Applied Psychology of Business BIOVALENTIA: Biological Research Journal Golden Ratio of Mapping Idea and Literature Format Golden Ratio of Auditing Research Journal Of Human And Education (JAHE) Golden Ratio of Data in Summary Journal of Artificial Intelligence and Digital Business Journal of Law, Education and Business Kajian Akuntansi Jurnal Medika: Medika Biosaintifika: Journal of Biology & Biology Education

Bambang Sugiharto

Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Jember

Author-ID : 388822

Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Effect of Drought Stress on Morphology and Chlorophyll Content of Sorghum (Sorghum bicolor L. Moench) Rahardhianto, Arsetyo; Raharjo, Sulistyo Yusuf; Nugroho, Sandi Luqman; Purwinto, Rahmanaji Setyo; Soleh, Mohamad Badrus; Sugiharto, Bambang
Jurnal ILMU DASAR Vol 26 No 1 (2025)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v26i1.32122

Sorghum is a type of cereal plant that easily adapts to stressful environmental conditions. Sorghum has many benefits as it can be used as food, feed, and energy sources, making it a promising crop for development on dry land in Indonesia. Drought impacts plants during their growth process, causing physiological and biological changes. This study aims to determine the effect of drought stress on the morphology and chlorophyll content of sorghum plants. Preliminary research was conducted by planting three varieties of sorghum (Numbu, Super 1, and Super 2). Subsequent planting involved selecting the variety most resistant to drought based on data analysis of several parameters. The basic design used in this experiment was a completely randomized design (CRD) with one factor: the level of drought stress, which had four levels -no drought stress as a day 0 (control) and drought stress for 3, 6 and 9 days by withholding water. The results showed a decrease in the total chlorophyll content of the leaves over time. Drought stress for 6 and 9 days without watering resulted in water content dropping below 25%, indicating severe drought stress and disrupting plant growth. Severe drought stress was characterized by low soil and leaf water content, yellowing and curling of the leaves.

The Effect of Concentration and Exposure Time Acyclovir for Elimination Sugarcane Mosaic Virus (SCMV) on The Apical Bud Culture of Sugarcane PS 881 Maisaro, Maisaro; Sugiharto, Bambang; Dewanti, Parawita
Jurnal ILMU DASAR Vol 18 No 1 (2017)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

SCMV (Sugarcane Mosaik Virus) is sugarcane crop disease resulting in chlorosis in leaves with the formation of the colors yellow and green intermittents. Based on survey information obtained that the air observation of all varieties of sugarcane was already stricken with the virus SCMV. Even the most formidable attack is on PS881 varieties with the intensity of the attacks reached 80%, so that it is estimated will lose up to 40% of the harvest. The sugarcane is virus free can be obtained via organogenesis in tissue culture method directly on the apical meristem, somatic embryogenesis at, and also with the addition of khemoterapeutan (acyclovir).The workings of the khemoterapi materials are the chemotherapi will interfere with replication and synthesis of genetic material of the virus but also cause the same effect against the mechanism of synthesis of nuklet acid on plants hosts. This research aims to find the best concentration and exposure time the most good in eliminating viruses SCMV in the apical bud culture of sugarcane PS881, using the antiviral acyclovir in conditions of invitro, so that the resulting plant will be virus free. The methods used to detect the presence of the virus by using the two ways, the first is by serology test through the protein content of the virus / checking nucleic acid virus with ELISA and the second is by RT-PCR. The results of the analysis showed that the interaction between the concentration of chemotherapy and exposure time produces the best treatment in eliminating the virus was . treatment with acyclovirc oncentration of 20 ppm and exposure time of 5 weeks.

Construction and expression of Wee1 recombinant protein in Escherichia coli strain BL21 (DE3) Oktapan, Ladefa Primana; Maulani, Ardela Alief; Ermawati, Netty; Sugiharto, Bambang
Jurnal ILMU DASAR Vol 24 No 2 (2023)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v24i2.28232

Wee1 is a gene encoding for protein kinase that is located in the nucleus and it plays an essential role in determining the timing of mitosis. Overexpression of Wee1 in rice is resulting in increased plant size. However, the increasing plant size due to the increase of Wee1 protein expression is not elucidated. Immunodiagnostic using specific antibodies against Wee1 protein should be conducted to determine the role of the protein on cell division. This experiment was directed to construct Wee1 in an expression vector for synthesis of recombinant Wee1 protein using three strategies of construction. The three strategies were construction of full length (FL), deletion of putative binding site (BS), and deletion of N-terminal domain (ΔN) of Wee1. Restriction analysis with BamH1/Sal1 of FL-Wee1, BS-Wee1, and ΔN-Wee1 construct resulted in the different DNA fragments with molecular size at 1239, 1176, and 960bp, respectively. This result indicated that the Wee1 fragments have been successfully inserted in the expression plasmid which was further confirmed using DNA sequencing. Colony PCR analysis showed that Escherichia coli strain BL21 (DE3) has been transformed with the constructs. The protein analysis using SDS-PAGE revealed that the recombinant protein of Wee1 synthesized in the E.coli containing ΔN-Wee1 construct, but not in FL-Wee1 and BS-Wee1 constructs. The ΔN-Wee1 protein was synthesized in an insoluble fraction with a molecular size of 38.8 kDa which is the same as the size estimated using the software ExPASy. Interestingly, the level of synthesized ΔN-Wee1 protein was not induced by IPTG concentration. Collectively, the results indicated that the DNA construct of ΔN-Wee1 is suitable for recombinant protein production.

Enhancement of Regeneration Efficiency through Callus Induction Media Using 2,4-Dicholorophenoxyacetic acid in Indica Rice (Oryza sativa L. var. Ciherang) Azizah, Kunti Anis; Restanto, Didik Pudji; Sugiharto, Bambang
Jurnal ILMU DASAR Vol 18 No 2 (2017)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Indica rice variety Ciherang is the most planted variety in Indonesia, but the micropropagation technique is restricted because it is known has low regeneration frequency and included as recalcitrant cultivar for tissue culture and tranformation activities. One of solution to resolve that problem is developing a technique of somatic embryogenesis in callus of ciherang rice cultivar. The aims of study were to determine medium composition for inducting embryogenic callus in Ciherang rice and to know the effectivity of rice regeneration using callus as explant. The methods were included induction of embryogenic callus in callus induction media (CIM) containing MS basal, Proline 600 mg/l, Casein Hidrolisat 300 mg/l, phytagel 2,5 g/l, BAP 0.25 mg/l, sukrosa 30 %, and 2,4-D in different concentration, from 2,4-D 0 mg/l as control (CIM 1), 2,4-D 2 mg/l (CIM 2), 2,4-D 3 mg/l (CIM 3), and 2,4-D 4 mg/l (CIM 4). It then be continued to regenerate the calli in RM 1 medium containing MS basal, NAA 0,2 mg/l, Kinetin 2 mg/l, Agarose 10 g/l, and sukrosa 30 %, pH 5,8 for six days in dark and RM2 medium containing MS basal , NAA 0,2 mg/l, Kinetin 2 mg/l, Agarose 8 g/l, sukrosa 30 %, pH 5,8 in light room. Results showed CIM 4 medium using 2,4-D 4 mg/l gave optimum result in calli induction with procentage 57,63% and CIM 3 using 2,4-D 3 mg/l gave optimum result in embryonic calli induction with procentage 53,63%. Result of embryonic calli gave regeneration frequency procentage is 9,6%. The total planlet obtained after five weeks old in regeneration medum were 11 planlets ready for aclimatization. Keywords: Somatic embryo, Indica rice (Oryza sativa L. var. Ciherang), Callus

TRANSFORMASI GEN SOSPS1 PADA TANAMAN TEBU OVEREKSPRESI GEN SOSUT1 EVENT 2 MENGGUNAKAN AGROBACTERIUM TUMEFACIENS Media Ningtyas, Rinda; Sugiharto, Bambang; Utarti, Esti
BERKALA SAINSTEK Vol 3 No 1 (2015)
Publisher : Universitas Jember

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Gen SoSPS1 (Saccharum officinarum sucrose phosphate synthase 1) merupakan gen pengkode enzim SPS yang berperandalam biosintesis sukrosa pada organ fotosintesis. Gen SoSUT1 (Saccharum officinarum sucrose transporter1) merupakangen pengkode protein SUT1 yang berperan pada proses transportasi sukrosa dari organ fotosintesis (source) ke organnonfotosintesis (sink). Tujuan penelitian ini adalah untuk mendapatkan tanaman tebu overekspresi ganda yaitu gen SoSPS1dan gen SoSUT1 melalui transformasi gen SoSPS1 pada tanaman tebu overekspresi gen SoSUT1 menggunakan A.tumefaciens. Hasil analisis PCR diperoleh tanaman tebu yang positif overekspresi ganda gen SoSUT1 dan gen SoSPS1sebanyak 4 tanaman pada transformasi ke-1, 3 tanaman pada transformasi ke-2 dan 4 tanaman pada transformasi ke-3.Efektivitas rata- rata transformasi gen SoSPS1 menggunakan A. tumefaciens yang mengandung konstruk plasmid pCL4-SoSPS1 dan dikendalikan oleh promoter RUBQ2 pada tanaman tebu overekspresi gen SoSUT1 sebesar 4,59%.

TRANSFORMASI GEN SOSUT1 PADA TANAMAN TEBU MENGGUNAKAN AGROBACTERIUM TUMEFACIENS STRAIN GV 3101 DAN PANGKAL TUNAS TEBU IN VITRO Edia, Edia F.D; Sugiharto, Bambang; Utarti, Esti
BERKALA SAINSTEK Vol 2 No 1 (2014)
Publisher : Universitas Jember

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Gen SoSUT1 merupakan gen pengkode protein sucrose transporter yang memfasilitasi proses transpotasi sukrosa dari jaringan fotosintetik (source) ke jaringan pengguna (sink) pada tanaman tebu. Tujuan penelitian ini adalah mendapatkan tanaman tebu transforman melalui transformasi genetik menggunakan vektor Agrobacterium tumefaciens yang membawa gen SoSUT1. Eksplan pangkal tunas tebu in vitro diinfeksi dengan A. tumefaciens yang membawa konstruk pAct-SoSUT1 dan dilakukan seleksi pada media MS dengan penambahan antibiotik hygromycin.. Tanaman putatif transforman yang telah berhasil melewati proses seleksi dan diaklimatisasi, dilakukan isolasi DNA genom kemudian dianalisis PCR. Hasil analisis PCR dengan pasangan primer 1F/1R hpt II menunjukkan bahwa dari 24 tanaman tebu putatif transforman, didapatkan 15 tanaman tebu positif mengandung gen SoSUT1. Efektifitas rata-rata transformasi gen SoSUT1 menggunakan eksplan pangkal tunas tebu in vitro sebesar 6,8%. Kata Kunci: Agrobacterium tumefaciens, SoSUT1, sukrosa.

Digital Economy and Finance Integration in Contemporary Indonesia Sugiharto, Bambang; Harkim, Harkim; Rejekia V Simanungkalit; Ilhamsyah Siregar; Maya Andriani
Jurnal Mantik Vol. 8 No. 2 (2024): August: Manajemen, Teknologi Informatika dan Komunikasi (Mantik)
Publisher : Institute of Computer Science (IOCS)

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The digital economy is one of modern system. That is an aspect of the economy based on the utilization power of high-tech approach. Clearly that is about empowerment of digital information and communication technology. In our contry, the digital economy system is growing very fast along with the increase of large market potential actually. The purpose of this research is to find out how the role of the digital business economy for the development of the modern market economy in Indonesia. The method used is using library research or library study (library research). The results of this study indicate that the presence of digital system presented of quality, such as better of negotiation, business style, and modern markets but the regular markets still needed for people of Indonesia.

Harnessing Digital Intelligence: Enhancing Effectiveness in Digital Marketing Sugiharto, Bambang; Harkim, H.; Hiya, Nirmadarningsih; Simanungkalit, Rejekia Vaizal; Andriani, Maya
Golden Ratio of Marketing and Applied Psychology of Business Vol. 5 No. 1 (2025): July - January
Publisher : Manunggal Halim Jaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52970/grmapb.v5i1.770

This qualitative study explores the dynamics of digital entrepreneurship and marketing, aiming to provide insights into the multifaceted nature of the digital economy. Employing a systematic literature review and thematic analysis methodology, the research delves into the role of platform-based business models, digital marketing strategies, and emerging trends within the digital landscape. The study reveals that platform ecosystems play a pivotal role in driving value creation and innovation, serving as catalysts for entrepreneurship by providing essential infrastructure and opportunities for value exchange. Additionally, it emphasizes the significance of data-driven decision-making and marketing analytics in optimizing digital marketing strategies, enabling businesses to deliver personalized experiences and drive engagement across multiple channels. Key findings include the transformative implications of platform-based business models for various stakeholders, the importance of user-centric design principles in enhancing customer experiences, and the challenges posed by regulatory compliance and technological integration. The study contributes to theoretical understanding by elucidating the complexities of digital entrepreneurship and marketing, while also offering practical implications for entrepreneurs to navigate the competitive digital marketplace successfully.

PERGESERAN PARADIGMA: PADA SAINS, FILSAFAT DAN AGAMA SAAT INI Sugiharto, Bambang
MELINTAS An International Journal of Philosophy and Religion (MIJPR) Vol. 26 No. 3 (2010)
Publisher : Faculty of Philosophy, Parahyangan Catholic University, Bandung

The article seeks to elicit some current tendencies that have made science, philosophy and religion in the third millennium change their self-perception, hence a paradigm shift. The hermeneutic point of view, the bias of gender, the negative impacts, the critique of the ideological presupposition behind each field, along with their respective internal evolution, have all put science, philosophy and religion into a redefinition and recontextualization. By now science has recognized the limits of its own logic and language, and seems to see the possibility of different kinds of ‘logic’ used by other types of knowledge. Philosophy, after Heideggerian phenomenology, has seemed to lose the possibility of creating an ‘all encompassing’ system of thought, and instead has to be more modest in simply identifying hermeneutically various modes of knowledge and their interconnection with pre-reflective modes of being, as well as in uncovering the root of today’s problems of life and humanity. As for religion, to revitalize itself today it has to shift its basic orientation, from doctrine and its propositional truth-claim to the complexity of religious experience, from ‘what one believes’ to ‘what one does’. Keywords :*Flux, *Web, *Relasional, *Ideologis, *Logika, *Lebenswelt, *hermeneutik, *narasi besar, *pra-reflektif, *doktrin, *pengalaman, *religion without religion

INTERCULTURAL ENCOUNTER AND CHRISTIANITY TODAY Sugiharto, Bambang
MELINTAS An International Journal of Philosophy and Religion (MIJPR) Vol. 25 No. 2 (2009)
Publisher : Faculty of Philosophy, Parahyangan Catholic University, Bandung

Intercultural encounter is something inevitable and crucial today. Its significance for religions depends on how religions conceive of intercultural translatability and the meaning of ‘the other’. Concerning the former, there are three possibilities : different cultures can be seen as radically untranslatable, mutually translatable in terms of universal economic medium, or mutually translatable in terms of universal doctrinal message. Each brings its own consequences. Concerning the latter, the other may be viewed as the outer-other or the inner-other of which both require some kind of self-relativization on the part of religion. If Christianity is consistent with its ‘logic of love’, it would be governed by heteronomous reason in which the self lives from out of itself , whereas its dwelling place is not the privileged centre.

Co-Authors A Bagus Nur Sudrajat A. Dinar Ambarwati Agung Nur Probohudono Agus Marwan Agustien, Inyana Dwi Agustien, Inyana Dwi Ahmad Hidayat Ahmad Ilham Tanzil Ai Sumirah Setiawati Amir, Muhammad Hisyam Andriani, Maya Anita Firmanti Anjas Wida Elistia Rini Anuary Dwi Rosyid, Wafa Prasetia Arif Syaifudin, Arif Ariyono, Alief Rizky Asshidiqie, Wahyu Pratama Aventi Aventi, Aventi Azizah, Kunti Anis Bambang Subiyanto, Bambang Bambang Sugiharto Barus, Muhammad Arif Bayati, Tutiana Bon, Abdul Thalib Bin Budi Sulistiarini, Emma Dany Cahyadi De Hans, Nickytha Linch Dedi Dwilaksana Dedy Eko Rahmanto Dewita, Tiara Ajeng Dian Al Ghifari Perwitasari Digdo Listyadi, Digdo Djenal . Dr. Hadrianus Tedjoworo, S.Ag., STL. Dwi Djumhariyanto Edia F.D Edia, Edia F.D Edison Edison Edy Listanto Elizabeth Eneng Tita Tosida Eny I. Riyanti Erlia Narulita Esti Utarti Fajrianto, Sigit Fanata, Wahyu Indra Duwi Fatimah Zahara Fibriani, Suwinda Gaol, Nickytha Linch De Hans Lumban Gulo, Desi Natalia Hafiz, Syauqi Abyan Hafizi, Muhamad Halawa, Herry Martinus Hari Purnomo HARKIM Harkim, H. Harkim, Harkim Hasibuan, Aqil Muhammad Hayati, Nur Fadillah Hiya, Nirmadarningsih Husin, Andriati Amir Icih, Icih Ika Oktavianawati Ilhamsyah Siregar Imas Cintamulya Insani, Vivian Nurul Intan Ria Neliana Inyana Dwi Agustien Irawan Gulo, Sastra Darma Iryani Endah Febrianti Jayus Jayus Kesuma, Muhammad Yudha Kurnia Indriyanti Purnamasari, Kurnia Indriyanti Kurniawan , Asep Kusmana Kusmana Laily Ilman Widuri, Laily Ilman Laksono Trisnantoro Lasino Lasino, Lasino Lassa, Anita Lathifah Hanum, Lathifah Liem, Jhon Sepron Defretus Liliek Sulistyowati Lilik Yulianingsih Lina Maulidiana Maesaroh, Nurlaila Magnon, Cassidy Maisaro, Maisaro Marina Pakaya Marwan, Agus Maulani, Ardela Alief Maulida, Dinda Mala MAYA ANDRIANI Media Ningtyas, Rinda MISWAR - Mochamad Asrofi Mohd Radzi, Fathin Nuril A'isha Binti Muhammad Hazmi Muhammad, Adibillah Mukhamad Su'udi Nadya Oktafiana Neliana, Intan Ria Netty Ermawati Nikmah, Nasilatun Nina Oktaria, Nina Ningtiyas, Wulan Nursyiam Nirmadarningsih Hiya Nirwana Br Bangun Nugroho, Sandi Luqman Nur Basuki Nurfatimah, Siti Nurhayati Nursely, Dinda Oktapan, Ladefa Primana Pandiangan, Jenny Ester Parawita Dewanti Purnama Okviandari, Purnama Purwinto, Rahmanaji Setyo Putri, Trisandi Eka Rachman Setiawan Rahardhianto, Arsetyo Raharja, Ajung Gilang Putra Raharjo, Sulistyo Yusuf Rahma Rei Sakura Ramadhan, Mohamad Ibnu Ramadhani, Rifki Fahrisal Ratnasari, Tri Ratri Ciptaningtyas Rejekia V Simanungkalit Restanto, Didik Pudji Rizal, Asep Rizki, Ainur Salahuddin Junus Salih, Yasir Sari , Indaniaty Hasanah Sigit Soepardjono Simamora, Harkim Simanungkalit, Rejekia Simanungkalit, Rejekia V Simanungkalit, Rejekia V. Simanungkalit, Rejekia Vaizal Sinambela, Khayriza Sirait, Syah Paradiba Siregar, Ilhamsyah Siregar, Luthfi Hidayat Siti Arifah Siti Rohimah Siti Umayyah Slameto Slameto Soleh, Mohamad Badrus SRI AYU MADE Sri Mulyati Sri Mulyati Sri Panca Setyawati Sri Rejeki Subandi, Kotim Sugara, Victor Ilyas Sugianto, Efendi Sugiarto, Aan Sukardiman Syafina Pusparani Tanjung, Yulia Tiara Toto Hadiarto Tri Agus Siswoyo TRI HANDOYO Tri J. Santoso Tri Ratnasari Ummi Sholikhah Untung Murdiyanto Wahyu Nurkholis Hadi Syahputra Widhi Dyah Sawitri Widhiya Ninsiana, Widhiya Wulan Nursyiam Ningtyas Yanti Setianti Yayuk Fatmawati YENNI SAMRI JULIATI NASUTION, YENNI SAMRI JULIATI Yoga Yuniadi Yudi Rinanto Yundari, Yundari Yusuf Rachmandika

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