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All Journal Vegetalika AGRITROP International Journal of Biosciences and Biotechnology Jurnal Ilmu Dasar JURNAL TEKNOLOGI LINGKUNGAN AL KAUNIYAH BERKALA SAINSTEK Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Agrointek Indonesian Journal of Agricultural Science Jurnal AgroBiogen Buletin Penelitian Tanaman Rempah dan Obat ROTOR: JURNAL ILMIAH TEKNIK MESIN Indonesian Journal of Biotechnology UNEJ e-Proceeding Agriprima : Journal of Applied Agricultural Sciences Jurnal Bioteknologi & Biosains Indonesia (JBBI) ANNALES BOGORIENSES Mesin Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Jurnal Pengabdian Magister Pendidikan IPA J-Dinamika: Jurnal Pengabdian Kepada Masyarakat Jurnal Informasi dan Teknologi Jurnal JTIK (Jurnal Teknologi Informasi dan Komunikasi) Buletin Penelitian Tanaman Rempah dan Obat Agroradix : Jurnal Ilmu Pertanian International Journal of Quantitative Research and Modeling BIOVALENTIA: Biological Research Journal Journal of Law, Education and Business Biosaintifika: Journal of Biology & Biology Education
Bambang Sugiharto
Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Jember
Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Social Sciences

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TRANSFORMASI GEN SOSPS1 PADA TANAMAN TEBU OVEREKSPRESI GEN SOSUT1 EVENT 2 MENGGUNAKAN AGROBACTERIUM TUMEFACIENS Media Ningtyas, Rinda; Sugiharto, Bambang; Utarti, Esti
BERKALA SAINSTEK Vol 3 No 1 (2015)
Publisher : Universitas Jember

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Abstract

Gen SoSPS1 (Saccharum officinarum sucrose phosphate synthase 1) merupakan gen pengkode enzim SPS yang berperandalam biosintesis sukrosa pada organ fotosintesis. Gen SoSUT1 (Saccharum officinarum sucrose transporter1) merupakangen pengkode protein SUT1 yang berperan pada proses transportasi sukrosa dari organ fotosintesis (source) ke organnonfotosintesis (sink). Tujuan penelitian ini adalah untuk mendapatkan tanaman tebu overekspresi ganda yaitu gen SoSPS1dan gen SoSUT1 melalui transformasi gen SoSPS1 pada tanaman tebu overekspresi gen SoSUT1 menggunakan A.tumefaciens. Hasil analisis PCR diperoleh tanaman tebu yang positif overekspresi ganda gen SoSUT1 dan gen SoSPS1sebanyak 4 tanaman pada transformasi ke-1, 3 tanaman pada transformasi ke-2 dan 4 tanaman pada transformasi ke-3.Efektivitas rata- rata transformasi gen SoSPS1 menggunakan A. tumefaciens yang mengandung konstruk plasmid pCL4-SoSPS1 dan dikendalikan oleh promoter RUBQ2 pada tanaman tebu overekspresi gen SoSUT1 sebesar 4,59%.
TRANSFORMASI GEN SOSUT1 PADA TANAMAN TEBU MENGGUNAKAN AGROBACTERIUM TUMEFACIENS STRAIN GV 3101 DAN PANGKAL TUNAS TEBU IN VITRO Edia, Edia F.D; Sugiharto, Bambang; Utarti, Esti
BERKALA SAINSTEK Vol 2 No 1 (2014)
Publisher : Universitas Jember

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Abstract

Gen SoSUT1 merupakan gen pengkode protein sucrose transporter yang memfasilitasi proses transpotasi sukrosa dari jaringan fotosintetik (source) ke jaringan pengguna (sink) pada tanaman tebu. Tujuan penelitian ini adalah mendapatkan tanaman tebu transforman melalui transformasi genetik menggunakan vektor Agrobacterium tumefaciens yang membawa gen SoSUT1. Eksplan pangkal tunas tebu in vitro diinfeksi dengan A. tumefaciens yang membawa konstruk pAct-SoSUT1 dan dilakukan seleksi pada media MS dengan penambahan antibiotik hygromycin.. Tanaman putatif transforman yang telah berhasil melewati proses seleksi dan diaklimatisasi, dilakukan isolasi DNA genom kemudian dianalisis PCR. Hasil analisis PCR dengan pasangan primer 1F/1R hpt II menunjukkan bahwa dari 24 tanaman tebu putatif transforman, didapatkan 15 tanaman tebu positif mengandung gen SoSUT1. Efektifitas rata-rata transformasi gen SoSUT1 menggunakan eksplan pangkal tunas tebu in vitro sebesar 6,8%. Kata Kunci: Agrobacterium tumefaciens, SoSUT1, sukrosa.
Pelatihan Aplikasi Zat Pengatur Tumbuh Paclobutrazol pada Lima Varietas Tanaman Padi di Kecamatan Ledokombo, Kabupaten Jember Tanzil, Ahmad Ilham; Fanata, Wahyu Indra Duwi; Sholikhah, Ummi; Ratnasari, Tri; Sugiharto, Bambang; Nikmah, Nasilatun; Raharja, Ajung Gilang Putra; Asshidiqie, Wahyu Pratama; Ramadhani, Rifki Fahrisal; Muhammad, Adibillah; Ariyono, Alief Rizky; Neliana, Intan Ria; Hari Purnomo; Yusuf Rachmandika; Syafina Pusparani; Wahyu Nurkholis Hadi Syahputra
Jurnal Pengabdian Magister Pendidikan IPA Vol 8 No 1 (2025): Januari-Maret 2025
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v7i3.8316

Abstract

Tanaman padi yang menghasilkan beras merupakan komoditas utama di Kecamatan Ledokombo. Kendala yang dihadapi petani dalam budidaya termasuk cekaman abiotik berupa angin kencang pada musim penghujan. Hal ini mengakibatkan tanaman padi lokal dengan postur tinggi rentan mengalami rebah. Untuk mengatasi masalah ini, diperlukan pelatihan terkait penggunaan zat pengatur tumbuh guna mengendalikan pertumbuhan tanaman yang tinggi. Paclobutrazol (PBZ), sebagai salah satu jenis zat pengatur tumbuh, berperan penting dalam mengurangi tinggi tanaman. Pelatihan dilakukan langsung di lahan petani dengan menggunakan lima varietas padi yang berbeda. Hasil aplikasi menunjukkan bahwa padi yang ditanam oleh petani menjadi lebih pendek dibandingkan yang tidak diberi perlakuan serupa.
Modeling Queue Length at The Toll Gate Using Promodel Before and After Ramp-Off Construction Hafizi, Muhamad; Hafiz, Syauqi Abyan; Sugiharto, Bambang; Tosida, Eneng Tita; Bon, Abdul Thalib Bin; Sugara, Victor Ilyas; Subandi, Kotim; Salih, Yasir
International Journal of Quantitative Research and Modeling Vol 6, No 1 (2025)
Publisher : Research Collaboration Community (RCC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46336/ijqrm.v6i1.905

Abstract

In everyday life, queues often occur. Waiting at the counter to get train or movie tickets, at the toll gate, at the bank, at the supermarket, and in other situations that we often encounter Queues occur when the need for services exceeds the capacity or capacity of the service facility. As a result, users of the facility cannot get immediate service due to the busyness of the service. The Amplas Toll Gate queue is the object of this research. The Amplas Toll Gate is one of the densest toll gates that is heavily traveled by vehicles both entering and exiting. This makes it often seen a fairly long queue, especially during peak hours in the late afternoon to evening. The Medan City Government built an off ramp at the Amplas flyover in 2016. This off ramp leads directly to the Amplas toll gate. The vehicle arrival rate increases along with the queue length because vehicles can arrive faster to the toll gate. This study aims to calculate the queue length at the Amplas toll gate before and after the construction of the ramp off. Data is obtained by recording the volume of vehicles at the research location. With an average service time of 7 seconds, the queuing method produces a queue length of 11.98 meters, while the results using Pro Model software are 11.98 meters. In addition, the queue length after the construction of the ramp off decreased to 6.67 meters from before the construction of the ramp off. Promodel is a windows-based simulation software used to simulate and analyze a system.
Molecular Detection of Rust Fungus (Puccinia spp.) on Sugarcane using ITS region Tanzil, Ahmad Ilham; Maulida, Dinda Mala; Fanata, Wahyu Indra Duwi; Neliana, Intan Ria; Sugiharto, Bambang; Magnon, Cassidy
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 3 (2025): November 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i3.32946

Abstract

Identifying pathogenic fungi at the species level based on morphology, especially for leaf rust, is quite difficult. Further investigation, such as molecular techniques, is required to identify leaf rust fungi precisely. One way that can be employed is DNA barcoding. This study seeks to detect leaf rust fungi (Puccinia spp.) on sugarcane. Accurate species identification allows early detection of Puccinia kuehnii, helping farmers and extension agencies to apply targeted control strategies. A fungal sample was collected from sugarcane fields in Bondowoso, East Java, Indonesia. Morphological identification was done with an SEM, whereas molecular identification was done through DNA extraction, amplification, visualization, sequencing, and phylogenetic tree creation. The primers employed are obtained from the ITS region of ribosomal DNA, which contains enough diversity to differentiate fungi at the species level. The amplification findings revealed a DNA band of 500 bp at both loci. Sequencing results indicate that samples The sequencing results indicatethat the samples from Bondowoso are closely related to the Puccinia kuehnii sequence from NCBI. The findings of the phylogenetic tree construction revealed that samples from both locations are still associated with P. kuehnii sequences from other nations. This is the first study documenting P. kuehnii in Indonesia, especially East Java, that integrates morphological and molecular characterization.
Optimasi Optical Density (OD) Dan Dosis Kanamisin Dalam Transformasi Gen Sucrose Phosphate Synthase (SoSPS1) Pada Anggrek Dendrobium sp. Arsy Chairiyah; Bambang Sugiharto; Purnama Okviandari; Parawita Dewanti; Khiar Ayatina Hasbi
Vegetalika Vol 14, No 4 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.106229

Abstract

Genetic transformation in plants involves the insertion of isolated foreign genes into the plant genome. Sucrose Phosphate Synthase (SPS) is a key enzyme in sucrose biosynthesis, the primary sugar serving as an energy source and carbon transport molecule in most plants. Dendrobium orchids, which utilize Crassulacean Acid Metabolism (CAM), often exhibit a slow growth rate, thereby limiting their commercial potential. The insertion of the SPS gene into the orchid genome is expected to optimize the sucrose metabolic pathway, potentially influencing the rate of photosynthesis, carbon allocation, and growth characteristics of the orchid. Optimizing the optical density (OD) of the Agrobacterium suspension and the dosage of kanamycin is crucial for enhancing the success rate of the gene transformation process. This study aimed to determine the optimal concentrations of OD and kanamycin to achieve efficient SoSPS1 gene transformation in orchids. Three-month-old Protocorm-Like Bodies (PLBs) of Dendrobium Glory White were used as the explant source. The transformed explants were subsequently evaluated for the effectiveness of the kanamycin dosage, the percentage of explant shoots, the number of shoots per explant, the transformation efficiency, and phenotypic changes in the transformed plants. The results showed that a transformation efficiency of up to 40% was achieved using an Agrobacterium suspension at an OD600nm of 0.4. A lethal dose (LD50) of 100 mgL-1 kanamycin resulted in 34% of plantlets surviving post-selection. Transformation with the SoSPS1 gene in Dendrobium sp. induced distinct phenotypic plants including split leaves, swollen stems (pseudobulb), and increased shoot multiplication.
Perubahan Karakter Agronomi, Fisiologi, dan Biokimia pada Beberapa Varietas Tebu Selama Cekaman Kekeringan Muslimah Arniyanti; Risky Mulana Anur; Bambang Sugiharto; Tri Handoyo
Vegetalika Vol 15, No 1 (2026)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.112692

Abstract

Climate change in Indonesia has led to an increase in abiotic stress, particularly drought, which significantly affects sugarcane productivity. This research was conducted from 2024 to 2025 at the Laboratory of Molecular Biology and Biotechnology, Waste Management Unit, and Integrated Laboratory, University of Jember. The aim of this study was to evaluate the agronomic, physiological, and biochemical responses of five sugarcane varieties under drought stress conditions. The results showed that drought stress had varying impacts depending on the tolerance of each variety. The drought-tolerant transgenic variety (NXI4T) exhibited more stable responses, as indicated by lower drought and leaf rolling scores, higher soil water content (SWC) and relative water content (RWC), and relatively stable levels of chlorophyll, phenolics, and flavonoids. In addition, lower H₂O₂ accumulation and a stronger root system supported better adaptation to drought conditions.
Peningkatan Aktivitas Enzim Metabolisme Sukrosa dan Glikolisis pada Tanaman Padi Transgenik Overekspresi Gen SoSPS1 Ferdi Hariyanto; Bambang Sugiharto; Netty Ermawati; Wahyu Indra Duwi Fanata
Vegetalika Vol 15, No 2 (2026): In Publish
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.114798

Abstract

Sucrose Phosphate Synthase (SPS) is a key enzyme in the sucrose biosynthesis pathway and plays an essential role in regulating sucrose accumulation in plants. Overexpression of the SPS gene has been reported to enhance sucrose accumulation and growth in various plant species. However, its effects on the dynamics of sucrose-metabolising and glycolytic enzymes in rice remain insufficiently explored. This study aimed to analyze the activities of sucrose metabolism enzymes, including SPS, invertases (acid and neutral), sucrose synthase (SuSy), and the glycolytic enzyme hexokinase (HXK) in transgenic rice plants overexpressing the SoSPS1 gene. The experiment was conducted using a completely randomized design with five genotypes, one of which was the wild type, and four transgenic rice lines, T4, T6, T8, and T9, with four replicates. The results showed that overexpression of the SoSPS1 gene led to significant increases in plant height and biomass, as well as markedly enhanced SPS activity in all transgenic rice plants compared to the wild type. Invertase activity (A-INV and N-INV) did not differ significantly among genotypes. In contrast, SuSy activity increased dramatically in transgenic lines T6, T8, and T9, suggesting that sucrose degradation in response to the genetic modification is primarily regulated through SuSy. The increase in SuSy activity was accompanied by the rise in HXK activity in the same lines (T6, T8, and T9). These results indicate that HXK subsequently phosphorylate hexose sugars, which may act as substrates for cellulose synthesis and activate the transcription factors that lead to higher biomass production. Overall, this study demonstrates that overexpression of SoSPS1 influences carbon flux and sugar metabolism, providing deeper insights into the relationship among sucrose biosynthesis, sugar sensing, and biomass enhancement in transgenic rice.
Pengaruh Metode Isolasi DNA terhadap Kuantitas dan Kualitas Hasil Ekstraksi Genom Padi dan Tebu Ahmad Ilham Tanzil; Intan Ria Neliana; Wahyu Indra Duwi Fanata; Raudhotun Jamila; Bambang Sugiharto; Ummi Sholikhah; Tri Ratnasari
AGRORADIX : Jurnal Ilmu Pertanian Vol. 9 No. 2 (2026): Januari-Juli 2026
Publisher : Agroteknologi Fakultas Pertanian Universitas Islam Darul 'Ulum (UNISDA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52166/agroteknologi.v9i2.12535

Abstract

Teknik isolasi DNA merupakan salah satu metode dasar dalam bidang biologi molekuler yang bertujuan untuk memisahkan DNA dari komponen seluler lainnya. Memperoleh DNA berkualitas tinggi dengan hasil yang baik merupakan faktor pembatas dalam analisis genetik tanaman, karena sulit untuk memperoleh DNA berkualitas tinggi dalam jumlah yang memadai. Penelitian ini kami lakukan untuk mengoptimalkan teknik isolasi DNA genomik dari sampel tanaman, dengan tujuan memastikan kualitas dan kuantitas DNA yang diisolasi cukup untuk analisis molekuler. Metode yang digunakan melibatkan perbandingan teknik ekstraksi menggunakan metode mekanis dan metode gerus. Daun tebu dan padi, baik dalam kondisi basah maupun kering, digunakan sebagai sampel. Hasil penelitian menunjukkan peningkatan signifikan dalam konsentrasi dan kualitas DNA saat sampel basah digiling menggunakan proses gerus. Berdasarkan perbandingan absorbansi pada A260/A280 nm dan A260/A230 nm, serta hasil uji nanodrop, proses penggerusan menghasilkan kualitas dan konsentrasi DNA yang lebih tinggi. Dibandingkan dengan metode mekanis, hasil visualisasi PCR menunjukkan bahwa metode gerus dapat mengekspresikan pita DNA yang lebih tebal pada sampel daun padi. Sementara itu, terdapat beberapa variasi dalam ekspresi pita DNA pada sampel daun tebu. Data kuantitatif dihasilkan dari temuan visualisasi PCR. Metode gerus terbukti menghasilkan jumlah dan kualitas DNA sampel terbaik, berdasarkan hasil penelitian.
Isolasi dan Identifikasi Khamir Toleran Alkohol dari Molase Nurhayati; Jay Jayus; Anjas Wida Elistia Rini; Bambang Sugiharto; Dedy Eko Rahmanto
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 7, No 1 (2022): February 2022
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v7i1.5426

Abstract

Khamir mampu menghasilkan bioetanol dari molases. Selama proses produksi (proses fermentasi) terjadi proses akumulasi alkohol sehingga konsentrasi alkohol semakin meningkat. Konsentrasi etanol yang melebihi 8% (v/v) dapat menyebabkan ketidakstabilan membran yang kemudian dapat menurunkan permeabilitas membran dan homeostasis sel. Penelitian ini dilakukan isolasi dan identifikasi khamir indigenus dari molase yang toleran terhadap alkohol. Karakterisasi morfologi meliputi bentuk koloni, bentuk sel dan tipe pertunasa, sedangkan karaktersasi fisiologis dilakukan dengan kit API 20C. Identifikasi molekuler dilakukan dengan PCR dan sequencing DNA menggunakan primer ITS1-ITS4. Hasil penelitian didapatkan dua isolat khamir yaitu isolat X dan Z yang memiliki potensi toleran terhadap alkohol. Berdasarkan hasil karakteristik fisiologis, isolat X dan isolate Z memiliki kedekatan dengan Candida tropicalis. Hasil sequencing DNA dengan primer ITS1-ITS4 dan analisis dengan program blastn menunjukkan bahwa isolat X teridentifikasi sebagai spesies Candida parapsilosis strain AUMC 10714 dan isolat Z teridentifikasi sebagai spesies Candida parapsilosis ZA012.
Co-Authors A Bagus Nur Sudrajat A. Dinar Ambarwati Agustien, Inyana Dwi Agustien, Inyana Dwi Ahmad Ilham Tanzil Ahmad Ilham Tanzil Amir, Muhammad Hisyam Anjas Wida Elistia Rini Anuary Dwi Rosyid, Wafa Prasetia Arif Syaifudin, Arif Ariyono, Alief Rizky Arsy Chairiyah Asshidiqie, Wahyu Pratama Azizah, Kunti Anis Bayati, Tutiana Bon, Abdul Thalib Bin Budi Sulistiarini, Emma Dedi Dwilaksana Dedy Eko Rahmanto Dewita, Tiara Ajeng Dian Al Ghifari Perwitasari Digdo Listyadi, Digdo Djenal . Dwi Djumhariyanto Edia F.D Edia, Edia F.D Edy Listanto Elizabeth Eneng Tita Tosida Eny I. Riyanti Erlia Narulita Esti Utarti Fajrianto, Sigit Fanata, Wahyu Indra Duwi Ferdi Hariyanto Fibriani, Suwinda Hafiz, Syauqi Abyan Hafizi, Muhamad HARDIAN SUSILO ADDY Hari Purnomo Hasibuan, Aqil Muhammad Hayati, Nur Fadillah Husin, Andriati Amir Ida Ayu Putri Darmawanti Ika Oktavianawati Imas Cintamulya Inyana Dwi Agustien Iryani Endah Febrianti Jayus Jayus Khiar Ayatina Hasbi Kusmana Kusmana Laily Ilman Widuri, Laily Ilman Laksono Trisnantoro Lasino Lasino, Lasino Lassa, Anita Liem, Jhon Sepron Defretus Magnon, Cassidy Maisaro, Maisaro Maulani, Ardela Alief Maulida, Dinda Mala Media Ningtyas, Rinda MISWAR - Mochamad Asrofi Muhammad Hazmi Muhammad, Adibillah Mukhamad Su'udi Muslimah Arniyanti Nadya Oktafiana Neliana, Intan Ria Netty Ermawati Netty Ermawati Ni Putu Ayu Erninda Oktaviani Suputri Nikmah, Nasilatun Nina Oktaria, Nina Ningtiyas, Wulan Nursyiam Nugroho, Sandi Luqman Nurhayati Oktapan, Ladefa Primana Parawita Dewanti Purnama Okviandari Purnama Okviandari, Purnama Purwinto, Rahmanaji Setyo Rachman Setiawan Rahardhianto, Arsetyo Raharja, Ajung Gilang Putra Raharjo, Sulistyo Yusuf Rahma Rei Sakura Ramadhani, Rifki Fahrisal Ratnasari, Tri Raudhotun Jamila Restanto, Didik Pudji Retnosari Apriasti Rindang Dwiyani Risky Mulana Anur Salahuddin Junus Salih, Yasir Sigit Soepardjono Siswahyudianto Siti Rohimah Siti Umayyah Soleh, Mohamad Badrus SRI AYU MADE Sri Rejeki Subandi, Kotim Sugara, Victor Ilyas Sugianto, Efendi Sukardiman Syafina Pusparani Toto Hadiarto Tri Agus Siswoyo TRI HANDOYO Tri J. Santoso Tri Ratnasari Tri Ratnasari Ummi Sholikhah Ummi Sholikhah Untung Murdiyanto Wahyu Indra Duwi Fanata Wahyu Indra Duwi Fanata Wahyu Nurkholis Hadi Syahputra Weny Nailul Hidayati Widhi Dyah Sawitri Wulan Nursyiam Ningtyas Yanti Setianti Yayuk Fatmawati Yoga Yuniadi Yudi Rinanto Yundari, Yundari Yusuf Rachmandika