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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Fitopatologi Indonesia Buletin Hama dan Penyakit Tumbuhan Jurnal Agrista JIK Jurnal Ilmu Komputer Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Ilmu Komputer dan Agri-Informatika Teodolita: Media Komunikasi Ilmiah di Bidang teknik Seminar Nasional Informatika (SEMNASIF) Jurnal Perlindungan Tanaman Indonesia Sinkron : Jurnal dan Penelitian Teknik Informatika Jurnal RESTI (Rekayasa Sistem dan Teknologi Informasi) Jurnal Ilmiah Universitas Batanghari Jambi Kinetik: Game Technology, Information System, Computer Network, Computing, Electronics, and Control JITK (Jurnal Ilmu Pengetahuan dan Komputer) IKRA-ITH Informatika : Jurnal Komputer dan Informatika Jurnal Informatika Universitas Pamulang Eduscience : Jurnal Ilmu Pendidikan Jurnal Pengabdian Masyarakat AbdiMas Astonjadro IKRA-ITH ABDIMAS AL-TANZIM : JURNAL MANAJEMEN PENDIDIKAN ISLAM JUTEKIN (Jurnal Manajemen Informatika) Simtek : Jurnal Sistem Informasi dan Teknik Komputer Aptisi Transactions on Technopreneurship (ATT) CCIT (Creative Communication and Innovative Technology) Journal Jurnal Teknologi Komputer dan Sistem Informasi Jurnal Fokus Elektroda : Energi Listrik, Telekomunikasi, Komputer, Elektronika dan Kendali JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Generation Journal Jurnal Tekinkom (Teknik Informasi dan Komputer) Budapest International Research and Critics Institute-Journal (BIRCI-Journal): Humanities and Social Sciences International Journal Of Science, Technology & Management (IJSTM) Fokus Elektroda: Energi Listrik, Telekomunikasi, Komputer, Elektronika dan Kendali) IAIC Transactions on Sustainable Digital Innovation (ITSDI) Jurnal Teknologi dan Sistem Tertanam Devotion: Journal of Research and Community Service Jurnal Minfo Polgan (JMP) Journal of Social Research Ilmu Komputer untuk Masyarakat Jurnal Locus Penelitian dan Pengabdian IKA BINA EN PABOLO : PENGABDIAN KEPADA MASYARAKAT Jurnal Indonesia Sosial Teknologi Jurnal Indonesia Sosial Sains Eduvest - Journal of Universal Studies Innovative: Journal Of Social Science Research Media Abdimas Jurnal penelitian jalan dan Jembatan Asian Journal of Social and Humanities Jurnal Ilmu Ekonomi, Pendidikan dan Teknik (IDENTIK) Jurnal Pengabdian kepada MASyarakat (J-PMAS) Innovative Research in Civil and Environmental Engineering (IRCEE) Global Insights in Management and Economic Research

Budi Tjahjono

UNIVERSITAS ESA UNGGUL

Author-ID : 404087

Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Transportation Veterinary Other

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Cloning of Genomic DNA Flanking Transposon in the Nonpathogenic Mutant of Xanthomonas axonopodis pv. glycines M715 ALINA AKHDIYA RUSMANA; ANTONIUS SUWANTO; BUDI TJAHJONO
HAYATI Journal of Biosciences Vol. 12 No. 2 (2005): June 2005
Publisher : Bogor Agricultural University, Indonesia

The objective of this work is to clone flanking DNA derived from Tn-5 mutagenesis of wild type strain Xanthomonas axonopodis pv. glycines as first step to clone and to identify the gene involved in pathogenicity mechanism. We have localized the flanking DNA fragment from a nonpathogenic mutant of Xag M715. Southern hybridization analysis using 2.8 kb EcoRI from pYR103 as a probe showed that the fragment is located within 2.0 kb PstI fragment. A 0.7 kb flanking DNA was amplified using inverse PCR technique, and inserted into pGEM-T Easy vector generating a 3.7 kb recombinant plasmid (pAA01). Southern hybridization analysis of the wild type (YR32) with pAA01 as a probe indicated a hybridization signal located at approximately 3.0 kb PstI fragment. DNA sequence analysis revealed that the DNA fragment has a 64% identity to a vir gene of Bacillus anthracis.

Rapid Detection of Bacterial Pustule Disease on Soybean Employing PCR Technique with Specific Primers ANDI KHAERUNI; ANTONIUS SUWANTO; BUDI TJAHJONO; MEITY SURADJI SINAGA
HAYATI Journal of Biosciences Vol. 14 No. 2 (2007): June 2007
Publisher : Bogor Agricultural University, Indonesia

A rapid polymerase chain reaction (PCR)-based procedure was developed for detection of Xanthomonas axonopodis pv. glycines, the causal agent of bacterial pustule disease on soybean. A set of primers was designed from partial sequence of the pathogenicity gene of X. axonopodis pv. glycines strain YR32. Specific PCR product of 490 base pairs was produced from strains of X. axonopodis pv. glycines originally from Indonesia as well as from Taiwan. No other pathovars and bacterial species among those tested showed amplification product under optimized PCR conditions. Shaking infected soybean leaves in phosphate buffer saline during six hours was proved to be an essential in order to increase cell number of the bacterial. The procedure was applicable and reliable for detecting of pathogens in infected plant materials. The procedure was proved to be more effective than that of conventional detection and could be of great help for monitoring of pustule bacterial disease in the soybean fields. Key words: Xanthomonas axonopodis pv. glycines, bacterial pustule disease, rapid detection, PCR, specific primer

Desain Pengembangan Bahan Ajar Digital Berbantuan Aplikasi Animaker Pada Mata Kuliah Pendidikan TIK (Teknologi Informasi dan Komunikasi) Faiz Fauzan Muhajir; Budi Tjahjono; Badri Munawar
JTKSI (Jurnal Teknologi Komputer dan Sistem Informasi) Vol 5, No 2 (2022): JTKSI (Jurnal Teknologi Komputer dan Sistem Informasi)
Publisher : JTKSI (Jurnal Teknologi Komputer dan Sistem Informasi)

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This research aims to see the effectiveness of the use of teaching material development. digitally assisted Animaker application for STKIP student Sheikh Manshur who took ICT Education courses The use of animation in the learning process is to enrich the creativity of students in the era of learning. Technology. The problem that occurs in the education process at STKIP Syekh Manshur is the lack of access. to operate digital-based teaching materials, although there are already available facilities such as laptops or a projector. the learning process carried out in ICT education in blanded play with learning using animated videos will make it easier for Students Enriching teaching materials. Researchers use the ADDIE (Analysis, Design, Develop, Implementation, and Evaluation). The research subjects were 30 STKIP Students sheikh Manshur who took ICT Education Courses The results of the research conducted at the Evaluation stage showed The success of 85.2% can be categorized as very high. Digital teaching materials Using the help of animaker application is considered effective for use by STKIP student Syekh Manshur

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Geminivirus infecting pepper: symptom variation and transmission study Infection of geminiviruses has been reported to cause signz3cantyield loss on various crops. Polymerase chain reaction was able to detect geminivirus infection on pepper samples around Bogor and Cipanas, West Java. Symptom variation and transmission of the geminivirus infectingpepper was studied using five solanaceae plants with three dzgerent transmission mannec i.e. mechanical inoculation, side grafirng, and insect vector. The transmission study showed that mechanical inoculation was not able to transmit the eminivirus, while side grafting and insect vector, Bemisia tabaci, caused infection with symptoms varies from yellow mosaic, leaf curl leaf distortion, to stunting of the plant. Higher infection was observed on pepper var. Hot Chilli through B. tabaci (8009) and side grafting (71.4%) than those on chilli pepper and tomato. DNA fiagment of - 1.7 kb was amplified wing PCRfim those plants showing symptoms, but no DNA fiagment was observedfrom symptomless eggplant and tobacco var. White Burley. Thus it can be concluded that the last two kina3 ofplants were resistant to the geminivirus infectingpepper.

It has been known that phytoplasma inducing witches broom disease can infect various kind of leguminous plants in Indonesia, among others soybean. Information on the resistance of various kind of soybean varieties to the phytoplasma is still limited. Experiments were conducted to eveluate the resistance of ten soybean varieties/lines to the phytoplasma. The evaluation was carried out by inoculation method through Orosius argentatus Evans, with two days of acquisition feeding period, ten days lat Asniwita Asniwita; Rusmilah Suseno; Sri Hendrastuti Hidayat; Budi Tjahjono
Buletin Hama dan Penyakit Tumbuhan Vol. 11 No. 2 (1999): Buletin Hama dan Penyakit Tanaman
Publisher : Buletin Hama dan Penyakit Tumbuhan

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It has been known that phytoplasma inducing witches broom disease can infect various kind of leguminous plants in Indonesia, among others soybean. Information on the resistance of various kind of soybean varieties to the phytoplasma is still limited. Experiments were conducted to eveluate the resistance of ten soybean varieties/lines to the phytoplasma. The evaluation was carried out by inoculation method through Orosius argentatus Evans, with two days of acquisition feeding period, ten days latent period and two days inoculation feeding period. Observation was conducted on the presence of phytoplasma, incubation period, symptom and number & weight of seed. The result indicated that line Malang 3474 was resistant, Sindiro and Sriono were tolerant, while the other tested varieties/lines, i.e. Galunggung, Orba, Ringgit, Wilis, Malang 2999, Malang 2805 and Sicinang were susceptible

Komunitas Mikrofungi pada Lapisan Horizon Serasah Acacia mangium Samingan Samingan; Lisdar I. Sudirman; Dede Setiadi; Alex Hartana; Budi Tjahjono
Jurnal Agrista Vol 12, No 2 (2008): Volume 12 Nomor 2 Agustus 2008
Publisher : Fakultas Pertanian, Universitas Syiah Kuala

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Microfungal Community on Litter Horizon Layer of Acacia mangiumABSTRACT. Fungal diversity on litter horizon layer of Acacia mangium were investigated to examined fungal species and fungal community on each litter horizon layer, and also to examined relationship between organic content of litter and fungal community. Twenty two species were isolated from three litter horizon layer with dilution method. Total fungal population on five years old A. mangium standing was higher than two years old, whereas on logging former area was low. Total fungal population on standing two and five years old were highest on L layer follow by F and H layer, but on logging former area were highest F layer followed by L and H layer. Aspergillus was dominate on H layer in almost of sampling collection area, beside that Aspergillus and Penicillium were found also on L and F layer. Generally L and F layer dominated by Sp7, Sp5, Sp20, and Sp22. The highest diversity indice on two years old standing was found at different layer.; L and H on health and Genoderma attacked standing, whereas on standing five years old, highest diversity indices was found at L layer, but on logging former area highest diversity indices was found at H layer.

ARSITEKTUR TRADISIONAL JAWA BANYUMASAN PADA PENDAPA DUPLIKAT SI PANJI DI KOTA LAMA BANYUMAS BUDI TJAHJONO; R SITI RUKAYAH; EDWARD ENDRIANTO
Teodolita ( Media Komunikasi Ilmiah di Bidang Teknik ) Vol 16, No 2 (2015)
Publisher : Fakultas Teknik Universitas Wijayakusuma Purwokerto

Kota Banyumas dahulu ibu kota karesidenan Banyumas, menjadi pusat pemerintahan HindiaBelanda. Pada pusat pemerintahannya diutara alun-alun terdapat pendapa yang di sakralkan PendapaSi Panji, dibangun Tumenggung Yudanegara II sekitar tahun 1743.Pendapa ini bermodel â€JogloTajugâ€, sebutan lokal yang ganjil, tidak dikenal pada bentuk arsitektur tradisional Jawa. Disini dicaribagaimana bentuk â€œJoglo Tajugâ€ itu dan apa penyebabnya ? Karena letaknya diperbatasan Jawa Barat,dan di Banyumas terjadi akulturasi Jawa dan Sunda. maka diduga ini percampuranarsitekturtradisional Jawa dengan Sunda ,atau mungkin adanya pengaruh lain dimasa lalu. Disini dibuktikan halitu dengan meneliti Pendapa Si Panji, hasil penelitiannya di kaji dengan teori Sejarah, BudayaBanyumasan, arsitektur tradisional Jawa, dan Sunda. Didapatkan bentuk Pendapa Si Panji berupaatap Tajug yang seharusnya sama sisi dipaksakan diatas denah Joglo yang empat persegi panjangsehingga menjadi bentuk yang ganjil, dan bentuk Joglo Tajug diduga tercipta sebagai protes alaBanyumasan terhadap raja Jawa masa itu.Kata kunci : Javanese Banyumasan, Joglo, Tajug

PERAN TEKNOLOGI KOMUNIKASI DAN INFORMASI TERHADAP KEKUATAN STAKEHOLDER DALAM MEWUJUDKAN EDEMOCRACY DI INDONESIA Budi Tjahjono
Seminar Nasional Informatika (SEMNASIF) Vol 1, No 6 (2009): E-Democracy
Publisher : Jurusan Teknik Informatika

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Peranan Information and Communnication Technology (ICT) telah banyak diketahui adalah untuk efisiensi, efektivitas dan transparans. E-Democracy di Indonesia belum berjalan dengan baik. ICT diharapkan dapat merubah pola pikir dalam melakukan pesta demokrasi, transparansi yang ditawarkan ICT bisa mengurangi kecurangan yang banyak dilakukan selama ini. Stakeholder sangat memengaruhi sukses atau tidaknya pelaksanaan e-Democracy di Indonesia. Kekuatan stakeholder dalam mensukseskan perlu adanya sosialisasi sehingga mempunyai power dalam mewujudkan e-Democracy. Masing-masing aktor dalam stakeholder bisa mengubah situasi dan kondisi tercapainya e-Democracy yang baik. Apabila dilakukan perubahan dari voting tradisional ke e-voting, maka peranan local administrasi sangat berpengaruh.

Keragaman Genetika Xanthomonas axonopodis pv. glycines Asal Kedelai Varietas Edamame di Indonesia Andi Khaeruni; Antonius Suwanto; Budi Tjahjono; Meity S. Sinaga
Jurnal Perlindungan Tanaman Indonesia Vol 13, No 1 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11814

Xanthomonas axonopodis pv. glycines cause bacterial pustule disease caused a serious disease in Edamame cultivation in Indonesia. We collected a total of 29 X. axonopodis pv. glycines isolates from Edamame fields at Jember, Ciawi, Cipanas and Bogor. The genetic diversity analysis of all isolates employing ARDRA and ISR technique showed six and seven different DNA profile, respectively. Therefore there are at least seven strains of X. axonopodis pv. glycines infected Edamame in Indonesia. Both CPI from Cipanas and JA4 from Sukorejo Jember isolates possess unique DNA profle and genetically are not closely related to other isolates.

Evidence for a Link Between Pathogenicity and the Role of ImpBacterial Transport Effector Proteins in Soybean Infection by Xanthomonas axonopodis pv. glycines ANTONIUS SUWANTO; ARIS TRI WAHYUDI; BUDI TJAHJONO
Microbiology Indonesia Vol. 1 No. 2 (2007): August 2007
Publisher : Indonesian Society for microbiology

Xanthomonas axonopodis pv. glycines (Xag) is the causal agent of bacterial pustule disease of soybeans. A nonpathogenic mutant of Xag (M715) was constructed employing transposon mutagenesis which showed similar epiphytic survival in planta to its wild type strain (YR32). The objective of this work was to identify and to analyze genes involved in pathogenicity in Xag YR32. Inverse Polymerase Chain Reaction (IPCR) was used to isolate the DNA flanking transposon insertion. A 1.3 kb flanking DNA fragment was sequenced and analyzed employing BLAST program to study homology, the position of transposon insertion and to predict the structure and function of the gene. One of the Open Reading Frames (ORFs) shared homology with inner membrane proteins (imps) of Xanthomonas axonopodis pv. citri (GenBank accession No. NC 003919). Northern blot analysis revealed that an imps gene was monocistronic and the size of imps mRNA in YR32 was slightly longer than in M715. Reverse Transcriptase- PCR analysis demonstrated that the imps transcript in M715 was much less abundant than in the wild type YR32. Transposon (mini-Tn5-Kmr-Tpr) was determined to be inserted close to the end of C-terminal region of imps gene and might be sufficient to destabilize the imps transcript in M715 and so influence effectors transportation from Xag to plant cell.

Co-Authors *, Hairuduin A.A. Ketut Agung Cahyawan W Adhi Fernandes Gamaliel Adhy, Dewanto Rosian Adigunawan, Adigunawan Adilah Widiasti Aep Saepudin Ahmad Fuad Ahmad Mutedi Aji Setiawan Akbar, Habibullah Alami, Tegar Alex Hartana Alexander Alexander, Alexander ALINA AKHDIYA RUSMANA Alivia Yufitri AMARILA MALIK Andi Khaeruni Andi Khaeruni Andriana, Dian Angga VB Annazma Ghazalba Antonius Suwanto Anugerah Cahyo Adhi Ardiansyah, Miri Arfian, Muhamad Hadi Arfian, Muhammad Hadi Arief Ichwani Arif Pami Setiaji Aris Tri Wahyudi Arniansyah, Arniansyah Asniwita Asniwita Aulia, Faujiatul Azzam Robbani, Muhammad Bambang Irawan Basyarewan, Humairoh Bayu Sulistiyanto Ipung Sutejo Binastya Anggara Sekti Budi Aribowo Dadang Hermawan Dafa Rizqi, Herin Daniel Hutajulu Dede Setiadi Dermawan Zebua Dewi Marini Dewi, Riris Septiana Sita Diah Aryani Djunaedi, Bagas Dwi Guntoro Dyah Kusuma Anggraini Edi Kartawijaya EDWARD ENDRIANTO Eliza S. Rusli Erry Yudhya Mulyani Euis Heryati Evans, Richard Faiz Fauzan Muhajir Fatonah, Nenden Siti Febrianto, Haris Fernandes Gamaliel, Adhi Firdaus, M. Dzulfiqar Gerry Firmansyah Gerry Firmasyah Ghazalba, Annazma Gilang, Destian Gunawan, Sholeh Gusti Fachman Pramudi Hadjarati, Panji Ramadhan Yudha Putra HAJRIAL ASWIDINNOOR Haryoto, Iin Sahuri Hendaryatna Hendaryatna Hendry Gunawawan Hermansyah Hermanto, Hermanto Hermawan, Deky Herwanto, Agus Hidayat, Muhamad Rifqi Huda, Nur Huda Hung Fei Imam Sutanto Ipung Sutejo, Bayu Sulistiyanto Irawan, Bambang Irdika Mansur Irsyadul Anam, Reza ISKANDAR ZULKARNAEN SIREGAR Islamy, Muhammad Rafli Ismiyati Meiharsiwi Jabar, Ajib Abdul Jefry Sunupurwa Asri Joniwan Joniwan Judianto Leihitu, Donny Dwy Juliana Juliana Kartini Kartini Kartini Kartini Khairurrahman, Rifqi Kikin H Mutaqin Klaski Putri, Krisanti Kundang Karsono Kundang Karsono Kundang Karsono Juman Kundang Karsono Juman Kusuma, Mahesa Adi Kusumah, Yayi Munara Laksono Trisnantoro Leihitu, Donny Dwy Judianto Lili Hastuti Lisdar Idwan Sudirman Lisdiana Lisdiana Lisdiana Lisdiana Lista Meria Luthfie Aldino Ismail M A Chozin Maemonah, Maemonah Malabay Malabay ., Malabay Malabay Malabay Malabay Malabay Martin Saputra, Martin Marzuki Pilliang Meiharsiwi, Ismiyati Meity S. Sinaga MEITY SURADJI SINAGA Millah, Shofiyul Mita, Vira Muhamad Bahrul Ulum Muhamad Bahrul Ulum Muhamad Hadi Arfian Muhammad Abdullah Hadi Muhammad Abdullah Hadi Muhammad Azzam Robbani Muhammad Bagaskara Muhammad Fajrul Aslim Muhtadi, Yudi Muksin, Andreanata Pradifta Munawar Munawar Munawar, Badri Mutedi, Ahmad Nabila, Efa Ayu Nainggolan, Restamauli br Narul Sakron Nasihin, Anwar Nasution, Tegar Alami Natadirja, Trenggana Nenden Siti Fatomah Nenden Siti Fatonah Nenden Siti Fatonah Niko Prasetyawan Aji Nila Rusiardi Jayanti Nina Nurhasanah Nindyo Artha Dewantara Wardhana Ningsih, Susilowati Nixon Erzed Nixon Erzed Nizirwan Anwar Nugraha, William Nur Widiyasono Nur Widiyasono, Nur Nurjannah Nurjannah Pamungkas, Ryan Tri Prabowo, Ary PURNAMA HIDAYAT Purwano SK Putra, Sipky Jaya Qiqi Asmara, Abdullah Qur'ani, Isti R SITI RUKAYAH Rahaman, Mosiur Rasyid, Ainur Ravie Kurnia Reza Irsyadul Anam Rian Adi Pamungkas Ridwan, Ridwan Rifqi Khairurrahman Riya Widayanti Rizk, Muhamad ROB HARLING Roesfiansjah Rasjidin Roesfiansjah Rasjidin Rohidin Rohidin, Rohidin RR. Ella Evrita Hestiandari Rudi Hermawan Rudi Hermawan Rudy Setiawan RUSMILAH SUSENO Rusnani * Ryan Putra Laksana Sabaruddin, Satria Sabri Alim Saepudin Saepudin Sakron, Narul Samingan Samingan Samuel Samuel SARASWATI, RIA Septian, Ferby Septianto, Dian Fajar Setiawan, Sony Shine Pintor Siolemba Patiro Sholeh Gunawan Siregar, Sarah Veronica Siti Fatomah, Nenden Sri H. Hidayat SRI HENDRASTUTI HIDAYAT Sri Husni Hidayati Suardana, Made Aka Sugeng Santoso Suhendar Suhendar, Suhendar Sularso Budilaksono Sulistyo, Catur Agus Supriyade Supriyade Supriyade, Supriyade Suryahim, Iim Tarigan, Masmur Tarigan, Masmur Tengku Riza Zarzani N Teras, Deni Tugiman Ummanah Ummanah, Ummanah Vivaldi Raditya Putra Wahyu Sabiqudin, Muhammad Wardhana, Nindyo Artha Dewantara Wibowo, Yudha Widiasti, Adilah Widodo, Agung Mulyo Wijaya, Jacob S William Nugraha Wisnu Ananta Kusuma Yessy Oktafriani Yudha Putra Hadjarati, Panji Ramadhan Yulhendri Yulhendri Yuliana Susanti Yuliati - Yunita Fauzia

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