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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Jurnal Veteriner OKARA: Jurnal Bahasa dan Sastra Jurnal Panorama Hukum Jurnal Ilmu Alam dan Lingkungan Scientific Journal of Reflection : Economic, Accounting, Management and Business Jurnal Medik Veteriner ARSHI Veterinary Letters Jurnal Dinamika UMT KREATIF : Jurnal Ilmiah Prodi Manajemen Universitas Pamulang JURNAL ILMIAH FEASIBLE (JIF) ZIRAA'AH MAJALAH ILMIAH PERTANIAN JURNAL SeMaRaK Journal of Parasite Science JURNAL ABDIMAS TRIDHARMA MANAJEMEN Jurnal Disrupsi Bisnis Abdimisi International Journal of Economy, Education and Entrepreneurship (IJE3) Indonesian Journal of Economy, Business, Entrepreneuship and Finance (IJEBEF) Journal of Economics and Business Letters Jurnal Bina Pengabdian Kepada Masyarakat Jurnal PKM Manajemen Bisnis International Journal of Multidisciplinary Research and Literature (IJOMRAL) AKADEMIK: Jurnal Mahasiswa Ekonomi & Bisnis AKADEMIK: Jurnal Mahasiswa Humanis Jurnal Ilmiah Mahasiswa (JIMAWA) Asean International Journal of Business Jurnal Indonesia Sosial Sains Pro Health Jurnal Ilmiah Kesehatan Jurnal Bisnis dan Manajemen West Science West Science Interdisciplinary Studies Kreativitas Pada Pengabdian Masyarakat (Krepa) West Science Interdisciplinary Studies Jurnal 7 Samudra Politeknik Pelayaran Surabaya Prosiding Seminar Hukum Aktual Fakultas Hukum Universitas Islam Indonesia Adpebi International Journal of Multidisciplinary Sciences Cakrawala: Jurnal Ekonomi, Manajemen dan Bisnis

Retno Wulansari

Departemen Klinik Dan Reproduksi, Fakultas Kedokteran Hewan Institut Pertanian Bogor, Jalan Agatis Kampus IPB Darmaga Bogor 16880

Author-ID : 420581

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Konsentrasi Serum Anjing yang Optimum untuk Menumbuhkan dan Memelihara Babesia canis dalam Biakan Tutuk Astyawati; Retno Wulansari; Cahyono -; Ferry Ardhiansyah; Ari Rumekso; Dhetty -
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The use of cultivation system in vitro is very important in the future study of Babesia canis. Theaim of this study was to cultivate B. canis in vitro using RPMI media with different concentration of dogsera. B. canis infected erythrocytes were collected from splenectomized infected dog. Parasites werecultivated with RPMI 1640 medium supplemented with normal dog sera at the concentration of 10%, 20%and 40%, the culture were then incubated in 5% CO2 , 37oC temperature for 17 days and subcultured every48 hours. The Percentage of Parasitized Erythrocytes (PPE) in culture with 10% dog serum was significantlylower than those 20%, and 40% The used of 20% and 40 % sera were better than 10%. It is recommendedthat 40 % serum can be used for initiation phase of cultivation, whereas 20% concentration were used formaintenance of the culture.

Dinamika Total Protein Serum Tikus Putih (Rattus novergicus) yang Diberi Mikrokapsul Imunoglobulin-G Anti-H5N1 (DYNAMICS OF TOTAL PROTEIN SERUM OF RATTUS NOVERGICUS GIVEN MICROCAPSULES OF IMMUNOGLOBULIN G ANTI H5N1) Kathirina Beatrik Riwu Wolo; Anita Esfandiari; Sri Murtini; Retno Wulansari
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Immunoglobulin G (IgG) from colostrum of hyperimmune colostrum obtained from vaccinated cattle with H5N1 vaccine is potential to be developed into passive immunotherapy product for “bird flu” infection. The administration of passive therapy can be done orally, but the environmental conditions of the digestive tract can cause IgG damage, so the microencapsulation technique was used in this study to protect IgG. This study aimed to evaluate the absobrption of H5N1 IgG anti-Avian Influenza which was microencapsulated using rats (Rattus novergicus) as animals model. This study was conducted using 108 adult male white rats (Rattus norvegicus), Wistar strain, approx. 250 grams of body weight. Rats were divided into three treatment groups (N = 36), i.e. control group (K) were given aqua distilled water, suspension group (S) were given anti-H5N1 IgG suspension, and microcapsules group (M) were given microcapsules equivalent to suspension with the titer of 25. Necropsy was carried out at four observation points (2, 4, 6 and 8 hours) to collected blood through the heart and collected in vacutainer without anticoagulants to obtain serum. The blood serum then analyzed for total protein and globulin and albumin/globulin ratio (A/ G). The results showed that the level of total protein and globulin group M was higher and significantly different (P> 0.05) compared to K and S at the end point of observation. The A/G ratio in group K showed a significant increase (P> 0.05) at the end point of observation while in group M there was a marked decrease (P> 0.05) at the time point of 2 hours to 4 hours and then constant until the end of observation. These results showed that administration of anti-H5N1 IgG microcapsules can increase total levels of protein and globulin in the blood.

Kejadian dan Terapi Babesiosis dengan Clindamycin pada Kucing (THE INCIDENCE AND TREATMENT OF BABESIOSIS WITH CLINDAMYCIN IN CAT) Retno Wulansari; Raden Roro Soesatyoratih; Suryono .
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The patients : 5 cats, came to “Klinik Hewan Cimanggu”, complains from the clients were includinglistlessness, anorexia, diarrhea, and constipation. From the Physical examination they showed a palemucous membrane, hyperaemic on sclera, larger of cranial abdominal . Laboratory finding on bloodssmear showed blood parasites in red cells that suspected as Babesia sp. One of them concurrently withhaemobartonella sp infections. The general result of blood laboratory test showed anemia andthrombocytopenia. Its treated by clindamycin (10 mg/Kg BW) and multivitamin twice a day for 3 weeksand parasitemia level in 1000 red cell was count before treated. Reexamination of smear red cell wasdone during and after treatment. In general they had demonstrated the decrease of parasitemia level.Some of them didn’t showed any changes of parasitemia level, however they showed morphological changesthat indicate inactive condition of parasites. The decrease of parasitemia level or the morphologicalchanges of parasites indicates that the development of parasitemia level has been depressed, so theclinical signs decreased and the animal’s condition improved. It believed that clindamycin inhibits proteinsynthesization in ribosome causing the damage to the parasite, but it will not eliminate the parasitesrapidly from peripheral blood. The Clindamycin treatment on cats with babesiosis will not induct the sideeffects .

Penurunan Kadar Gula Darah pada Monyet Ekor Panjang Obes dengan Pemberian Nikotin Dosis Rendah (DECRESE IN BLOOD SUGAR LEVEL IN LONG TAILED OBESE MACAQUES GIVING WITH LOW DOSE NICOTINE) Chusnul Choliq; Dondin Sajuthi; Irma Herawati Suparto; Dewi Apri Astuti; Retno Wulansari
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (133.242 KB)

The aim of the study was to evaluate the role of low dose nicotine on the profile of blood glucose and â-cell of pancreatic islets. Fourteen adult (aged 6 – 8 years) male cynomolgus monkeys grouped based ontheir Body Mass Index (BMI) into preobese (BMI=23.65 – 25.00) and obese (BMI e” 26.00) were used inthis study. Subsequently animals were grouped into four: (i) preobese monkeys with nicotine (pOb+), (ii)obese monkeys with nicotine (Ob+), (iii) preobese monkeys without nicotine (pOb-), and (iv) obese monkeyswithout nicotine (Ob-). Animals in the nicotine groups were fed with high fat diet mixed with nicotine dose0.5–0.75mg/kg body weight/day for three months and the others were fed Monkey Chow® only as thecontrol group. Blood samples were collected every month for glucose analysis and necropsy was performedat the end of study. Pancreas tissues were processed histologically and stained using immunohistochemicalmethod. The results showed that the blood glucose either preobese (28.37%) or obese (33.72%) animals inthe nicotine groups significantly decrease (p<0.05) during the study period in comparison to the controlgroup. Based on brown color intensity of granules cytoplasm of insulin producing cells or immunoreactiveâ-cells, it is shown that the cells of animals in the non nicotine group were more reactive than those in thenicotine groups. In conclusion, there was positive effect of low dose nicotine in maintaining the bloodglucose level in normal range by stimulation of islet cells proliferation to maintain the production ofinsulin in the pancreatic islet.

Ekhokardiografi Endokardiosis Penyakit Katup Mitral Jantung Anjing (ECHOCARDIOGRAPHY OF ENDOCARDIOSIS MITRAL VALVE HEART DISEASE IN DOGS) Deni Noviana; Retno Wulandari; Retno Wulansari
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Endocardiosis is a disease commonly found in Pomeranian dog characterized by progressive myxomatousdegeneration of the atrio-ventricular valves especially in the mitral valve. The purpose of this study was todefine the diagnose and severeity of this disease on the Pomeranian by using brightness mode, motion mode,dan color flow Doppler echocardiography technique. Echocardiography was performed on 8 Pomeranianconsisting of 6 males and 2 females with age range of 2-14 years. Brightness mode echocardiography wasused to see the echotexture of endocardium, mitral valve, and the valve movement. The results showedendocardium thickening, along with chronic fibrosis and nodular thickening of the anterior and posteriormitral valve leaflet. Three out of seven cases showed prolapsed of the mitral valve. Motion modeechocardiography was performed in order to measure left ventricle internal dimension, myocardium thickness,fractional shortening, left atrial and aortic dimension. The results showed myocardium thickening, alongwith left atrial enlargement. Color flow Doppler echocardiography was used to confirm the mitral valveregurgitation. Three of seven cases showed the presence of regurgitation signed by turbulence color of theprolapsed mitral valve. Based on the degree of severity, scoring system used in this study, endocardiosis canbe divided into three types that are mild, moderate and severe.

Keberhasilan Hiperimunisasi Pada Sapi Bunting: Kajian Terhadap Ketepatan Waktu Vaksinasi Safitria Wulandari; Anita Esfandiari; Sri Murtini; Retno Wulansari
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Penelitian ini bertujuan untuk melihat pengaruh umur kebuntingan induk sapi saat vaksin pertama kali diberikan terhadap keberhasilan hiperimunisasi. Sebanyak 12 ekor induk sapi bunting jenis sapi Friesian Holstein, dibagi menjadi kelompok kontrol dan vaksin, dengan masing-masing kelompok terdiri dari umur kebuntingan enam bulan (n=3) dan tujuh bulan (n=3). Induk sapi diinjeksi vaksin Avian influenza (AI) H5N1 komersial (killed vaccine) sub-kutan, sebanyak 5 dosis/ekor (106 EID50/dosis). Vaksinasi dilakukan dua kali dengan interval antar vaksinasi selama dua minggu. Sebelum vaksinasi, induk sapi diberi imunomodulator 0,1 mg/kg bb per oral selama tiga hari berturut-turut kemudian dilakukan priming dengan menyuntikkan antigen AI H5N1 inaktif tanpa adjuvant (intravena) tiga hari berturut-turut (10x28 HAU/ekor). Sampel darah dikoleksi melalui vena coccygea sebelum vaksinasi pertama, dua minggu pascavaksinasi I dan dua minggu pascavaksinasi II untuk dianalisis terhadap parameter leukosit (jumlah total leukosit dan limfosit), kadar total protein, globulin dan rasio albumin/ globulin (A/G), serta titer IgG anti AI H5N1 dalam darah dan kolostrum. Hasil pengamatan menunjukkan tidak terdapat perbedaan yang signifikan pada jumlah total leukosit dan limfosit, kadar total protein dan globulin, rasio A/G, serta titer IgG anti AI H5N1 dalam darah dan kolostrum, baik antar kelompok maupun antar waktu pengamatan (p>0.05). Namun demikian, pada induk sapi kelompok vaksin umur kebuntingan enam bulan menunjukkan parameter leukosit, kadar total protein dan globulin serta titer IgG anti AI H5N1 dalam darah dan kolostrum lebih tinggi dibandingkan kelompok induk sapi umur kebuntingan tujuh bulan. Umur kebuntingan pada saat vaksinasi pertama kali dilakukan tidak memengaruhi keberhasilan hiperimunisasi pada induk sapi bunting trimester akhir.

Kadar Protein Serum pada Sapi Peranakan Ongole di Balai Embrio Ternak Cipelang Bogor Putri Indah Ningtias; Sus Derthi Widhyari; Retno Wulansari
Jurnal Veteriner Vol 23 No 4 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.4.531

Salah satu plasma nutfah yang dimiliki oleh negara Indonesia yaitu sapi peranakan ongole (PO). Data dan nilai referensi normal kadar protein serum pada sapi PO yang digunakan sebagai donor dalam produksi embrio belum banyak dilaporkan. Tujuan penelitian ini adalah mengetahui gambaran protein serum yaitu albumin, globulin dan rasio albumin/globulin sapi PO induk yang digunakan sebagai donor sebelum produksi embrio. Penelitian ini menggunakan 10 ekor sapi PO, berumur 4-8 tahun dengan BCS 2,5-4,0. Sampel darah diambil melalui vena coccygea sebanyak 10 mL menggunakan jarum nomor 18-G. Serum dianalisis terhadap parameter protein total, albumin dan globulin dengan menggunakan alat spektrofotometer. Hasil penelitian menunjukkan bahwa rataan konsentrasi protein total, albumin, globulin, dan rasio A/G sapi donor PO masing-masing secara berurutan adalah 8,19±0,40 g/dL, 3,72±0,26 g/dL, 4,48±0,41 g/dL, dan 0,84±0,10. Data hasil penelitian ini merupakan data referensi kadar protein pada sapi PO induk. Hasil ini mendekati nilai referensi normal untuk jenis sapi yang lain. Kadar protein dapat dijadikan salah satu indikator dalam menentukan status kesehatan hewan sapi donor dalam upaya penyiapan produksi embrio.

Co-Authors . Suryono Aditya Primawidyawan Afton Atabany Agus Lelana Agus Wijaya Agus Wijaya Andalusi, Ratih Andiani, Paramita Andrea Citra Brilliantina Andriani, Jeni Andriansyah, Riva Anita Esfandiari Annastasya Yuliani Rahayu Ari Rumekso Arif Purwo Mihardi Arifin, Muhammad Zakka Putra Karim Samsul Arum Maula Hidayah Attabany, Afton Azzahrah, Putri Bagya Agung Prabowo, Bagya Agung BAHITS, ABDUL Bambang Pontjo Priosoeryanto Bayu Febram Bayu Firmala Kusuma Boedi Setiawan Cahyono - Chusnul Choliq Chusnul Choliq Citra Maharani Deni Noviana Deny Setyo Wibowo DEVI PARAMITHA DEWI APRI ASTUTI Dewi, Fitriya Nur Annisa Dewi, Winda Kusuma Dhetty - Dondin Sajuthi Dyah Ratnaningsih Effendi, Siti Aisyah Rahmalia Endang Sholehin Endang Suprihati erfandari, ovy Esti Suntari Eva Harlina Faidah, Thalita Farah Fauziah Septiani Febriana, Rafadila Ferry Ardhiansyah Fitri Handayani Fitri, Arni Diana Ghifari Lutfi Fauzi Gunanti . Hadi, Reza Khairul Haryodharu Putra Santoso Herwin Pisestyani Huda, Ade Naelul Husnul Hamdi I wayan Teguh Wibawan Indah Ayu Johanda Intan Permata Putri Irma Herawati Suparto Januarilla, Jahra’ Marwah Joesoef, Jayusman Arsiyanti Judijanto, Loso Kartjito, Nicolas Edward Christanto Kathirina Beatrik Riwu Wolo Kenny Astria Kristanti Ayuanita, Kristanti Lanniari, Nafrina Leni Maylina Mar'ah, Dian Latifathul Maria Rosalia Maswarni Meles, Dewa Ketut Mirnawati B Sudarwanto Mirnawati Sudarwanto Moh. Hafid Effendy, Moh. Hafid Mubarok, Ali Nabillah Anissa Nalanwal, Khayla Ningtias, Putri Indah Nur Fitriany, Neisa Nur Rahayu, Salsabila Nurfianti, Syifa Nurhasanah, Dila Padila Octovia , Carina Okabayashi, Tamaki Oktavianti, Nopi Pirwanto Midiani Poedji Hastutiek Prastiwi, Nurul Anjar Pratama, Muhammad Satria Resa Prianto, Joko Putikadyanto, Agus Purnomo Ahmad Putri Indah Ningtias Putri Indah Ningtias Putu Angga Wiradana Putu Eka Sudaryatma Putu Jodie Kusuma Wijaya Rachmi Ridho Raden Rara Sayyidati Alfi Ilmiah Putri Raden Roro Soesatyoratih Radya Rafi Setyawan Retno Sri Wahjuni Retno Wulandari Rini Dianti Fauzi Rostantinata, Riski S, Riri Sabina, Fitri Safitria Wulandari Salapudin, Salapudin Setyo Widodo Setyo Widodo Shafaa Istiana Nadzary Soeharsono Soeharsono Soesatyoratih, Roro Soleha Soleha Soleha Solinda, Gamelita Rizkawandi sri murtini . Subangkit, Mawar Suflani Sugeng Widodo Sugunavathy Palanisamy SULISTIANI, SINTA Sunanto Sunanto Surachmi Setiyaningsih Suryono Suryono . Sus Derthi Widhyai Sus Derthi Widhyari Sus Derthi Widhyarti Tabroni Tika Leoni Putri Tutuk Astyawati Tyasningsih, Wiwiek Ulhaq, Ziyad Wahyu Andri Wibowo Wolo, Kathirina Beathrik Riwu

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