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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Akuakultur Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Jurnal Kedokteran Brawijaya Jurnal Ilmu dan Teknologi Hasil Ternak The Journal of Experimental Life Sciences (JELS) AGRIKA Jurnal Harpodon Borneo Jurnal Kelautan : Indonesian Journal of Marine Science and Technology Jurnal Mahasiswa Manajemen Sumberdaya Perairan Wacana, Jurnal Sosial dan Humaniora Research Journal of Life Science REKAYASA Jurnal Sumberdaya Akuatik Indopasifik JFMR (Journal of Fisheries and Marine Research) Jurnal Penelitian Pendidikan IPA (JPPIPA) Samakia: Jurnal Ilmu Perikanan Indonesian Journal of Natural Pigments Journal of Aquaculture and Fish Health Syntax Idea Jurnal Karinov Jurnal Abdi Insani Jurnal Pengabdian, Pendidikan dan Teknologi Teknomekanik JURNAL BAHARI PAPADAK Livestock and Animal Research Jurnal Penelitian Chanos Chanos Jurnal Kedokteran Hewan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Journal of Fisheries & Marine

Yanuhar, Uun

Aquatic Resources Management, Fisheries And Marine Science Faculty, Universitas Brawijaya

Author-ID : 422230

Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Physics Social Sciences Veterinary Other

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Monitoring Kualitas Air pada Tambak Udang Vaname (Litopenaeus vannamei) di Perairan Pantai Utara Jawa Timur Nico Rahman Caesar; Uun Yanuhar; Andik Isdianto; Ekwan Nofa Wiratno; Lutfi Ni`'Matus Salamah; Zainal Abidin; Alfi Nur Rusydi; Umi Zakiyah; Hartati Kartikaningsih; Gilang Rusrita Aida; Lutfia Fahimatul Ilmi
Samakia : Jurnal Ilmu Perikanan Vol 16 No 1 (2025): Samakia: Jurnal Ilmu Perikanan
Publisher : Faculty of Science and Technology University Ibrahimy

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35316/jsapi.v16i1.6153

Penelitian ini bertujuan untuk mengevaluasi kualitas perairan di Pantai Utara Jawa Timur berdasarkan parameter suhu, salinitas, pH, Total Dissolved Solids (TDS), Total Suspended Solids (TSS), oksigen terlarut (DO), amonia, nitrat, dan ortofosfat. Penentuan lokasi sampling dilakukan menggunakan metode purposive random sampling di tiga lokasi, yaitu Kabupaten Sidoarjo, Gresik, dan Lamongan. Hasil pengukuran menunjukkan suhu perairan berkisar antara 29,5–31,7 °C, sesuai dengan standar optimal untuk biota laut (28–32 °C). Salinitas dan pH (7,3–7,6) berada dalam batas wajar, mendukung kehidupan organisme akuatik. Nilai TDS di bawah 1000 ppm, sementara rata-rata TSS adalah 10,4 mg/L, jauh di bawah ambang batas 20–80 mg/L. Konsentrasi DO berkisar 4,7–6,97 mg/L, hampir memenuhi standar optimal > 5 mg/L. Kandungan amonia (0,007–0,088 mg/L) dan nitrat (0,051–0,095 mg/L) berada dalam batas aman. Konsentrasi ortofosfat (0,025–0,123 mg/L) melampaui ambang batas 0,015 mg/L di beberapa lokasi, kemungkinan disebabkan oleh limbah domestik. Hasil penelitian ini menunjukkan kondisi perairan Pantai Utara Jawa Timur mendukung ekosistem akuatik, meskipun potensi ancaman dari aktivitas manusia memerlukan pengelolaan lebih lanjut untuk menjaga keberlanjutan ekosistem

Effect of Extracellular Crude Protein (ECP) on expression IL-1, IL-6, IFN- in nile tilapia (Oreochromis niloticus) Cucun Herlina; Mohammad Fadjar; Maftuch Maftuch; Uun Yanuhar
Depik Vol 14, No 3 (2025): SEPTEMBER 2025
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.14.3.43338

Modern strategies incorporate preventive measures such as extracellular products, as soluble proteins with antigenic properties, as well as generating a broad immune response. The aim of research is to determine and analyze the effect of the extracellular product of E. tarda on enhancing the immunity of Nile tilapia (O. niloticus). The research design with treatment durations of d0, 3rd, 6th, 9th day, and control (A, B, C, D, K). ECP vaccination was injected intraperitoneally at a dose of 0.1 ml/fish. The parameters analyzed are the expression of IL-1, IL-6, and IFN-, phagocytic activity, leukocyte differential, total white blood cells, and relative percent survival. The results of phagocytosis activity were highest in treatment C at 84.18% 1.81. The highest leukocyte differential in treatment C was lymphocytes at 56% 2.00, monocytes at 28% 2.00, and 16% 0.00, with a total leukocyte count of 29 x 10^3 cells 2.64, and RPS values ranging from 83.33% to 100% over 9 days of post-vaccination maintenance. Then, post-vaccination, there was an increase in the expression levels of the cytokine genes IL-1 and IL-6, but there was no increase in the expression levels of the IFN- gene compared to the control in Nile tilapia. (O. niloticus).Keywords:ECPIL-1IL-6IFN-Immune Response

The Impact of Heavy Metal Fe, Zn, and Cu in Water and Sediment on the Histopathology of Gills and Liver of Mullet Fish (Crenimugil seheli) at Kamal Beach, Madura Strait Kharisma Nuria Fitri; Muhammad Musa; Uun Yanuhar
Jurnal Penelitian Pendidikan IPA Vol 10 No 12 (2024): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i12.8196

Kamal Beach in the Madura Strait is suspected to have experienced heavy metal contamination due to ship recycling industry activities. Heavy metals have a negative impact on the aquatic environment, aquatic biota and even humans. This study aims to analyze the impact of heavy metal content in Kamal Beach, especially heavy metals Fe, Zn, and Cu as heavy metals found in the region on the histopathological aspects of the gill and liver organs of aquatic biota, namely mullet fish (Crenimugil seheli). The levels of heavy metals Fe, Zn, and Cu were analyzed using the AAS (Atomic Absorption Spectometry) method in accordance with SNI 6989.4:2009. Hiatopathological analysis of the gill and liver organs was carried out using the scoring method where from the results of the analysis it was known that the damage that occurred in the gill organs was in the form of edema, hyperplasia, lamellar fusion, and necrosis while damage to the liver organs included degeneration, necrosis, and congestion. Damage to the gill and liver organs of mullet fish is caused by the high content of Zn and Cu in water and sediment.

The Analysis of Hepatopancreas Histologycal Damage in Neocallichirus karumba (Poore and Griffin) Shrimp Caused by Heavy Metal Pb Exposure in Madura Strait Kristiani, Maria; Herawati, Endang Yuli; Yanuhar, Uun
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Graduate School, Universitas Brawijaya

Madura strait known as the second busiest shipping lanes in Indonesia. Human activities on the environment can influence the marine ecosystem derived from the household, fishery processing and capture fisheries. It can pollute coastal environments, one of which is heavy metal exposure such as Cd, Hg, Ca, As and Pb. These metals are greatly impacting the life of sea biota. The objective of this research, therefore, is to examine the hepatopancreas damage rate of shrimp Neocallichirus karumba due to the exposure of heavy metal Pb in Madura Strait. Method of research is by analyzing Pb content in water, sediment and shrimp by taking sample from 3 different stations. Hepatopancreas damage is then analyzed with SEM-EDX. Result of research indicates that at Station A, Pb pollution in water is the biggest and counted for 0.25 ppm, while those in sediment and shrimp are 5.85 ppm and 1.24 ppm. At Station B, Pb pollution in water is 0.19 ppm, whereas that in sediment is 5.51 ppm, but that in shrimp is 1.04 ppm. At Station C, Pb pollution in water counts for 0.18 ppm, and in sediment, it stands for 5.5 ppm but 0.02 ppm for shrimp. Result of analysis against hepatopancreas damage is explained as follows. At Station A, vacuolization is 20 % and Pb content in organ is 0.520 ppm. At Station B, the parameters are 10% and 0.196ppm. At Station C, it includes 15% and 0.173ppm. Organ damage is straightforwardly related to Pb content in water and sediment. Shrimp age is quite influential to the percentage of organ damage.Keywords: Heavy metal Pb pollution, hepatopancreas organ damage, Madura Strait, N. karumba shrimp.

Assessing the Genotoxic Potentials of Methomyl-based Pesticide in Tilapia (Oreochromis niloticus) Using Micronucleus Assay Islamy, R Adharyan; Yanuhar, Uun; Hertika, Asus Maizar Suryanto
The Journal of Experimental Life Science Vol. 7 No. 2 (2017)
Publisher : Graduate School, Universitas Brawijaya

Pesticides are recognized as serious pollutants in the aquatic environment with the potential to cause genotoxic on the aquatic organism, especially fish. The micronucleus (MN) assay has been used to evaluate genotoxicity of many compounds in polluted ecosystems such pesticides. The aim of this study to determine genotoxic effect of methomyl-based pesticide on tilapia (Oreochromis niloticus). Fish were exposed to six different concentrations base on range finding test (0 ppm, 3.2 ppm, 4.2 ppm, 6.5 ppm, 8.7 ppm and 10 ppm) of methomyl-based pesticide. The micronucleus were collected from peripheral blood erythrocyte of fish after 96 h exposure. Peripheral blood samples smears were stained with Giemsa, MN frequencies were counted and statistically analyzed using one-way ANOVA. The result of this study showed after 96 hours exposed to methomyl-based pesticide, at concentration 0 ppm causes 0% mortality, at concentration to 3.2 ppm causes 30% mortality, at concentration 4.2 ppm causes 60% mortality, at concentration 6.5 ppm causes 70% mortality, at concentration 8.7 ppm causes 80% mortality, at concentration 10 ppm causes 100% mortality of fish test. Lethal Concentration 50 (LC50 - 96 hours) of methomy-base pesticide towards tilapia (O. niloticus) is 4.015 ppm. Through micronuclei assay during 96 hour exposure of methomyl-based pesticide, the result shows that frequencies of micronuclei in erythrocyte of fish test at concentration at 0ppm is 12â€°, 18â€° and 16â€°; at concentration at 3.2ppm is 33â€°, 26â€° and 29â€°; at concentration at 4.2ppm is 41â€°, 38â€° and 46â€°; at concentration at 6.5ppm is 68â€°, 81â€° and 82â€°; at concentration 8.7ppm is 133â€°, 130â€° and 137â€°; at concentration 10ppm is 163â€°, 166â€° and 156â€°. It revealed that methomyl-based pesticide exposure induced after 96 h significantly (P<0.05) increased genotoxic potentials simultaneous with increased concentration.Keywords: Genotoxic, Methomyl, Micronucleus Assay, Pesticide, Tilapia.

Analysis of Genotype and Phylogenetic of Koi Herpesvirus (KHV) on Carp (Cyprinus carpio) in Lombok Island of West Nusa Tenggara Province Based on Thymidine Kinase Sequence Baihani, Amira; Yanuhar, Uun; Maftuch, Maftuch
The Journal of Experimental Life Science Vol. 8 No. 1 (2018)
Publisher : Graduate School, Universitas Brawijaya

Koi herpesvirus (KHV) is a virus that usually infects carp and koi causing mass mortality, as well as economic and social loss. The results of varied test are often found in its development. Allegedly, there are mutation or genetic variation of KHV in Indonesia. Based on these problems, this research aims to understand the genetic variation and kindship of KHV isolate in the carp (Cyprinus carpio) in Lombok Island of West Nusa Tenggara Province based on thymidine kinase sequence. Water quality of fish pond was checked. KHV clinical symptom was checked visually in each fish sample, then sacrifices the organ (gill) for PCR analysis and DNA sequencing. The research results show that clinical symptoms that appear are pale fish gill and bleeding on the surface of the body. The temperature in the research site shows an optimum range for KHV development. The results electrophoresis of carp suspected with KHV shows five positives out of a total six samples. Genetically, KHV isolates from Lombok Island of West Nusa Tenggara Province is identical with the GenBank isolate code KHV-J/U/I and Indo_0C05WNT2.Keywords: Carp, Koi herpesvirus, Phylogenetic, Thymidine kinase.

Expression of Hsp70 and β-actin Genes as The Immune Response against Viral Nervous Necrosis that Infected Asian Seabass (Lates calcalifer) Wahyudi, Yusuf Arif; Yanuhar, Uun; Maftuch, Maftuch
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Graduate School, Universitas Brawijaya

The viral nervous necrosis (VNN) is betanodavirus of the family Nodaviridae with acute infection and associated with high levels mortality up to 100% of numerous larval-stage marine and freshwater fish species. The danger signals of viral infection that are capable of activating APCs (Antigen Presenting Cells), furthermore produced molecules protein receptors such as interferon and heat shock proteins. Stimulating interferon type I (IFN I) induce several antiviral molecules, further binding the actin cytoskeleton to reach the site of infection. This study demonstrated a correlation between increased levels of Hsp70 (heat shock proteins) and actin filamentous (β-actin) within invasion wild-type isolate of ssRNA VNN from Asian Seabass (L. calcalifer) juvenile. Furthermore, expression of Hsp70 and actin as an indicator or biomarker of stressed states in fish. The reverse transcriptase polymerase chain reaction (RT-PCR) method used to finding expression Hsp70 and β-actin. Whereas nested RT-PCR used for VNN genes. The up-regulation of Hsp70 was observed on the brain tissue higher than eye tissue of sample positive infected VNN. Whereas different expression of β-actin, β-actin receptor expression tends to be stable in the organs of the brain and eye, both invaded VNN or normal, while on the eye, the ratio slightly increased based on the intensity of the band. The research shows that altered expression of heat shock protein 70 and beta-actin gene receptors in the target organs that response to invasion VNN at Asian seabass (L. calcalifer) juvenile. Alteration expression of heat shock protein 70 and beta-actin can be used as an indicator or biomarker of stress cells, especially from VNN invasion at fish. Keywords: Asian seabass, β-actin, Hsp70, Immune response, Viral nervous necrosis.

Potential of Dayak Onion (Eleutherine palmifolia (L) Merr) Extract As Antibacterial Against Pseudomonas fluorescens Fransira, Immaria; Yanuhar, Uun; Maftuch, Maftuch
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Graduate School, Universitas Brawijaya

Dayak onions (E. palmifolia (L) Merr) is a herb used as medicine and known to have antibacterial activity. This research aimed to evaluate antibacterial activity of Dayak onion (Eleutherine palmifolia (L) Merr) extract against fish pathogen Pseudomonas fluorescens. The method used in this study was extraction by maceration, UV-Vis spectrophotometry analysis on the extract and antibacterial activity test with Minimum Inhibitory Concentration (MIC) as well as disc diffusion test. The results of this study showed that the yield value was 8.87%. Based on UV-Vis analysis, it confirmed that extract of Dayak onion (Eleutherine palmifolia (L) Merr) contains predominantly flavonoids and phenols with its derivatives, that have antibacterial activity. The results of MIC and disc diffusion test showed potential antibacterial activity against P. fluorescens, as observed from the value of absorbance and inhibition zones that formed. Keywords: Antibacterial, Disc diffusion, Eleutherine palmifolia (L) Merr, MIC, Pseudomonas fluorescens.

The Effect of Protein Induction of Brachionus sp. Toward In-Vivo Response of Cluster Differentiation-8 (CD8) Cantang Grouper (Epinephelus sp.) Infected by VNN (Viral Nervous Necrosis) Nur Fauziyah Martiningsih; Uun Yanuhar; Mohammad Musa; Dwi Retna Kumalaningrum
Jurnal Penelitian Pendidikan IPA Vol 9 No 8 (2023): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i8.4025

The large demand for grouper conduce increase production intensively in various ways, by increasing the production of grouper aquaculture raises causing new problems, one of which is infection with VNN disease. The use of antibiotics to treat VNN infections is prohibited or no longer recommended by the government. An alternative way to deal with VNN infection is by increasing the body's immunity in the fish itself. One effort that can be done to increase the body's immunity in grouper is by administering Brachionus sp. protein extracts. This research was conducted to determine whether the Brachionus sp. has an effect on the immune system in cantang grouper (Epinephelus sp.) as indicated by the emergence of Cluster Differentiation-8 (CD8) expression in fish that have been infected with VNN. The proteins contained in Brachionus sp. that has three type molecular weight (122.73 kDa, 75.49 kDa, and 13.77 kDa) potential as an ingredient for treating VNN-infected fish. It is hoped that this can improve the immune system in Epinephelus sp. infected with VNN. Protein Brachionus sp. given by injection at a dose of 35 Âµl, 105 Âµl and 170 Âµl per 150 gr Epinephelus sp. 105 Âµl was the best concentration in boosting the immune system in the tested Epinephelus sp., as indicated by a decreased CD8 count.

The Relationship Between Water Quality and Phytoplankton Abundance with Different Showing Density in Litopenaeus vannamei Ponds in Bayeman Village, Probolinggo District, East Java Fika Fitrianesia; Asus Maizar Suryanto Hertika; Uun Yanuhar; Muhammad Faiq Ash Shiddiq
Jurnal Penelitian Pendidikan IPA Vol 10 No 2 (2024): February
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i2.4364

Intensive ponds often experience a decrease in water quality and cause ponds to bloom due to the fertility of the pond bottom due to the buildup of organic material originating from leftover feed, feces and the remains of dead shrimp. The aim of this research is to determine the relationship between water quality and phytoplankton abundance in intensive pond types with different densities in Bayeman Village, Probolinggo Regency. The research was carried out from January to March 2023. Samples were taken using the purposive sampling method in 6 different pond plots. Phytoplankton analysis was carried out at the Laboratory of the Sand Source Freshwater Fisheries Technical Implementation Unit and the Hydrobiology Laboratory of the Fish Resources Section of the Faculty of Fisheries and Marine Sciences. Measurement of in situ water quality parameters, namely temperature, pH, brightness, DO, TDS, light intensity, salinity and ex situ nitrate, phosphate and ammonia using spectrophotometric methods. The correlation test results of physico-chemical parameters related to phytoplankton abundance have a strong and heterogeneous relationship only with the orthophosphate parameter

Co-Authors Abdul Rahem Faqih Ach. Khumaidi Adelya Desi Kurniawati Adhitya, Dewa Sukma Trinanda Agus Susilo Agus Susilo Alfarisi, Muhammad Asnin Alfi Nur Rusydi Amasda, Naufal Rizky Aminnudin, Aminnudin Amira Baihani Amira Masitha Andi Kurniawan Andik Isdianto Anggraini, Rifka Rimbi Anisa Millah Taqiyyah Anjasmara, Aang Setyawan Annisa Fitri Anggreini Apri Supii Arief Farhan Kurniawan Anasiru Asep Awaludin Prihanto Asus Maizar Suryanto H Asus Maizar Suryanto Hertika Awalia Oktaviana Baihani, Amira Bili Darnanto Susilo BS, Sumitro Choirul Huda Choirul Huda Cucun Herlina Defa Rizqi Machfuda Diana Arfiati Dwi Retna Kumalaningrum Dyah Kinasih Wuragil Dyah Kinasih Wuragil Dyah Kinasih Wuragil Dyah Tri Rahayu Dyah Wuragil E Ernawati, E Eddy Suprayitno Edi Susilo Ekwan Nofa Wiratno Endang Yuli Herawati Fadjar, Mohammad Fajar Shodiq Fathah, Aulia Lanudia Fauliza, Tifa feri Setiawan Fika Fitrianesia Gatot Ciptadi Gede Angga Krishna Fariestha Gian Suryanatha Hartawan Gian Suryanatha Hartawan Gilang Rusrita Aida Gilang Rusrita Aida Gunanti Mahasri Hardoko Hardoko Hartati Kartikaningsih Herlina, Cucun Herly Evanuarini Herly Evanuarini Heru Suryanto Immaria Fransira Indah Wahyuni Abida Irawanto, Yovan Endik Islamy, R Adharyan Ismail, Mahrus Ita Fitria S Jati Batoro Jibril Maulana Kharisma Nuria Fitri Kristiani, Maria Lutfi Ni`'Matus Salamah Lutfia Fahimatul Ilmi MA, Widodo Maftuch ., Maftuch Maftuch Maftuch Maftuch Maftuch Maheno Sri Widodo, Maheno Sri Marsono Marsono Martiningsih, Nur Fauzi-yah Masitha, Amira Mohammad Bisri Mohammad Fadjar Mohammad Fadjar Mohammad Musa Mohammad Musa Mufidah, Rhobithotus Muhajir, Muhamad Muhammad Faiq Ash Shiddiq Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Sumsanto Muhammad Sumsanto Muhammad Sumsanto Musa, Mohammad Nezya Pramudya Wardani Nezya Pramudya Wardani Nico Caesar Nico Rahman Caesar Nizery, Sefhanissa Puspa Retno Nur Fauziyah Martiningsih Nur Fauziyah Martiningsih Nur Junirahma Nur Sakinah Junirahma Nusantara, Fajar Osman, Azlin Fazlina Pahlefi, Reval Pradana, Yanuar Rohmat Aji Purwoko, Agus Puspitasari, Intan Dwi Putri, Berlania Mahardika Ramadhanti, Nur Fa'izah Fitri Rifka Rimbi Anggraini Rika Wahyuningtyas Rika Wahyuningtyas Rika Wahyuningtyas, Rika Rizka Rahmana Putri Sanoesi, Ellana Sansi, Delfina Husniya Septi Anitasari Septi Anitasari, Septi Setyayunita, Tamaryska Sias, Quota Alief Soelistyoadi, Rachmat Noer Soemarno Soemarno Sri Andayani Sri Andayani Sri Andayani Andayani Sri Rahmaningsih Sri Rahmaningsih Sukarni Sukarni Suprayitno Suprayitno Susilowati, Surya Rachman Taufik Budhi Pramono Taufik Budhi Pramono Teotonio Gomes, Guilherme Tri Djoko Lelono Umi Zakiyah Utari, Inpita Casuarina Eqisetia Wahyudi, Yusuf Arif Wardani, Nezya Pramudya Wuragil, Dyah Kinasih Yenny Risjani Yovan Endik Irawanto Yusuf Arif Wahyudi Yusuf Wahyudi Zainal Abidin

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