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The Characterization of Haemaglutinin Protein of Vili Vibrio alginolyticus as Adhesion Molecule on Epithelial Cells Receptor of Humpback Grouper (Cromileptes altivelis) Yanuhar, Uun
Jurnal Kedokteran Hewan Vol 2, No 1 (2008): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v2i1.3499

Abstract

Vibriosis was a crucial problem and a primer infection causing mortality of humpback grouper.Virulence factor of vibrio, such as vili, is an adhesion molecule protein based on haemaglutination test toblood serum of humpback grouper. This research was aimed to characterize an adhesion molecule of viliprotein of V. alginolyticus on epithelial cells of Humpback grouper as haemaglutinin protein. Themethods are laboratory exploration by isolate epithelial cells of Humpback grouper, characterize a receptorand test vili protein to adhesion molecule that is epithelial cell of Humpback grouper. The result of proteinhaemaglutinin test to vili V. alginolyticus show that an adhesion molecule with a molecule weight of38.98 kDa is an adhesin vili V. alginolyticus and a haemaglutinin. The adhesion pattern of V.alginolyticus on epithelial cells of Humpback grouper exposed to protein haemaglutinin of vili show bothdiffuse and localize pattern. Research conclusion was the haemaglutinin protein of vili V. alginolyticuswas an adhesin having a role to both virulence process of bacteria and attachment process to receptor ofepithelial cells of Humpback grouper.Keywords: adhesion molecule, epithelial cell, vili, V. alginolyticus, C. altivelis
Immunological Response of Interleukin-6 on Cantang Hybrid Grouper (Ephinephelus sp.) By Induction Of Protein Brachionus sp. Kumalaningrum, Dwi Retna; Yanuhar, Uun; Musa, Mohammad; Martiningsih, Nur Fauzi-yah
Journal of Aquaculture and Fish Health Vol. 13 No. 3 (2024): JAFH Vol. 13 No. 3 September 2024
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v13i3.30835

Abstract

This study examines the role of Brachionus sp protein on the interleukin-6 pro-inflammatory immune system in Cantang grouper infected with Viral Nervous Necrosis (VNN). The purpose of this study was to determine the protein content of Brachionus sp. which has the potential as an antivirus and to find out the benefits of Brachionus sp. on the expression of Interleukin-6 as an indicator of increased fish body defense system against VNN. Testing of Brachionus protein by injection at doses of 35 µl, 105 µl, and 170 µl /150 gram Cantang grouper. The results showed that the lowest decrease was at a dose of 105 µl/150-gram Cantang groupers.  In addition, Brachionus sp. contains 3 protein bands with molecular weights of 122.73 kDa, 75.49 kDa, and 13.77 kDa. 
The Effect of Protein Induction of Brachionus sp. Toward In-Vivo Response of Cluster Differentiation-8 (CD8) Cantang Grouper (Epinephelus sp.) Infected by VNN (Viral Nervous Necrosis) Martiningsih, Nur Fauziyah; Yanuhar, Uun; Musa, Mohammad; Kumalaningrum, Dwi Retna
Jurnal Penelitian Pendidikan IPA Vol 9 No 8 (2023): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i8.4025

Abstract

The large demand for grouper conduce increase production intensively in various ways, by increasing the production of grouper aquaculture raises causing new problems, one of which is infection with VNN disease. The use of antibiotics to treat VNN infections is prohibited or no longer recommended by the government. An alternative way to deal with VNN infection is by increasing the body's immunity in the fish itself. One effort that can be done to increase the body's immunity in grouper is by administering Brachionus sp. protein extracts. This research was conducted to determine whether the Brachionus sp. has an effect on the immune system in cantang grouper (Epinephelus sp.) as indicated by the emergence of Cluster Differentiation-8 (CD8) expression in fish that have been infected with VNN. The proteins contained in Brachionus sp. that has three type molecular weight (122.73 kDa, 75.49 kDa, and 13.77 kDa) potential as an ingredient for treating VNN-infected fish. It is hoped that this can improve the immune system in Epinephelus sp. infected with VNN. Protein Brachionus sp. given by injection at a dose of 35 µl, 105 µl and 170 µl per 150 gr Epinephelus sp. 105 µl was the best concentration in boosting the immune system in the tested Epinephelus sp., as indicated by a decreased CD8 count.
The Relationship Between Water Quality and Phytoplankton Abundance with Different Showing Density in Litopenaeus vannamei Ponds in Bayeman Village, Probolinggo District, East Java Fitrianesia, Fika; Hertika, Asus Maizar Suryanto; Yanuhar, Uun; Shiddiq, Muhammad Faiq Ash
Jurnal Penelitian Pendidikan IPA Vol 10 No 2 (2024): February
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i2.4364

Abstract

Intensive ponds often experience a decrease in water quality and cause ponds to bloom due to the fertility of the pond bottom due to the buildup of organic material originating from leftover feed, feces and the remains of dead shrimp. The aim of this research is to determine the relationship between water quality and phytoplankton abundance in intensive pond types with different densities in Bayeman Village, Probolinggo Regency. The research was carried out from January to March 2023. Samples were taken using the purposive sampling method in 6 different pond plots. Phytoplankton analysis was carried out at the Laboratory of the Sand Source Freshwater Fisheries Technical Implementation Unit and the Hydrobiology Laboratory of the Fish Resources Section of the Faculty of Fisheries and Marine Sciences. Measurement of in situ water quality parameters, namely temperature, pH, brightness, DO, TDS, light intensity, salinity and ex situ nitrate, phosphate and ammonia using spectrophotometric methods. The correlation test results of physico-chemical parameters related to phytoplankton abundance have a strong and heterogeneous relationship only with the orthophosphate parameter
Hematological Analysis of Red Bader Fish (Barbonymus altus) as a Biomarker in Assessing Pollution Status in Brantas River, Surabaya, East Java Anasiru, Arief Farhan Kurniawan; Hertika, Asus Maizar Suryanto; Yanuhar, Uun
Jurnal Penelitian Pendidikan IPA Vol 10 No 2 (2024): February
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i2.5659

Abstract

The Brantas River has many functions for both humans and the aquatic organisms in it. The type of fish that is often found in the Brantas River in Surabaya is the red bader fish (Barbonymus altus). Hematology is a sensitive parameter and can be used as a marker in assessing water conditions. The aim of this research is to analyze the hematological profile of red bader fish (Barbonymus altus) to assess the condition of the Brantas River, Surabaya. The research was carried out at 3 locations on the Brantas River in Surabaya with 3 sampling times with an interval of 1 month starting in January – March 2022. The parameters observed were water quality (temperature, TSS, TDS, pH, DO, BOD, ammonia and heavy metals Hg) and hematological parameters (erythrocytes, hemoglobin, hematocrit, leukocytes and micronuclei). Analysis of the relationship between water quality parameters and hematology using the Correspondence Correlation Analysis (CCA) method. The results show that there are water quality parameters that do not meet quality standards and there are abnormal hematological parameters. CCA analysis shows that there is a correlation between water quality and hematological parameters with moderate concentrations (erythrocytes, hemoglobin and hematocrit) and strong concentrations (leukocytes and micronuclei).
Analisis Status Kualitas Air di Sungai Porong yang Tercemar oleh Deterjen terhadap Respon Hematologi Ikan Java Barb (Barbonymus gonionotus) Caesar, Nico Rahman; Yanuhar, Uun; Faqih, Abd. Rahem; Anitasari, Septi; Ciptadi, Gatot; Musa, Muhammad; Bisri, Mohammad; Sumsanto, Muhammad; Wardani, Nezya Pramudya
Jurnal Penelitian Pendidikan IPA Vol 10 No 6 (2024): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i6.6170

Abstract

The Porong River is one of the rivers that faces high anthropogenic impacts, such as surfactant waste from detergents. The hematology of Javanese carp (Barbonymus gonionotus) serves as a sentinel organism that can be used as a biomarker for the waters of the Porong River. The aim of this study is to analyze the water quality status of the Porong River polluted with surfactants using the STORET method, as well as its impact on the hematology of Javanese carp using regression and correlation analysis. The research employed a descriptive survey method. The results of the study indicated that the Porong River was categorized as moderately polluted. The average highest surfactant concentration reached 7.2 mg/L, leading to an increase in TSS to 107.95 mg/L and a decrease in DO to 1.80 mg/L. Surfactants exhibited a negative correlation with erythrocytes (91.2%), hematocrit (47.7%), and hemoglobin (60.6%), indicating that the higher the surfactant concentration, the lower the number of erythrocytes, hematocrit levels, and hemoglobin levels in Javanese carp. Conversely, there was a positive correlation between surfactants and leukocytes (56.7%) and blood glucose (66.7%), suggesting that the higher the surfactant concentration, the higher the number of leukocytes and blood glucose levels in Javanese carp.
KARAKTERISTIK SEDIMEN DI PERAIRAN PESISIR LEKOK, KABUPATEN PASURUAN, JAWA TIMUR Anggraini, Rifka Rimbi; Yanuhar, Uun; Risjani, Yenny
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 12 No. 1 (2020): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (573.681 KB) | DOI: 10.29244/jitkt.v12i1.28705

Abstract

Sedimentasi merupakan salah satu dinamika yang terjadi di kawasan pesisir untuk mengetahui kondisi pantai tersebut. Pesisir perairan Lekok merupakan salah satu kawasan perairan terbuka yang memiliki dinamika perubahan pesisir sangat fluktuatif. Studi sebaran jenis sedimen yang dilakukan secara terstruktur pada lokasi ini digunakan untuk memberikan informasi mengenai proses transportasi dan pengendapan sedimen berdasarkan distribusi ukuran butir sedimen. Penelitian ini bertujuan untuk mengetahui ukuran butir sedimen secara horizontal di pesisir perairan Lekok. Penelitian ini dilakukan pada sembilan titik sampling dengan pengambilan sampel berupa sedimen dasar secara acak dengan menggunakan metode grab sampler. Selanjutnya dilakukan analisis lebih lanjut dengan metode gravimetri. Berdasarkan hasil penelitian diketahui bahwa karakteristik sedimen dalam Pesisir perairan Lekok umumnya didominasi oleh tiga jenis sedimen yaitu: lempung berpasir, lempung dan lempung lanau. Nilai sorting berada dalam kondisi well-sorted hingga kondisi moderately well-sorted di mana kondisi sedimen didominasi dengan well sorted. Nilai skewness berada pada nilai skewed positively dimana nilai menunjukkan hasil positif. Nilai kurtosis berada pada leptokurtic dimana sedimen dari stasiun 1 ke stasiun 9 homogen.
Kajian Cemaran Mikroplastik Terhadap Profil Darah Ikan Bandeng (Chanos chanos) di Perairan Utara Jawa Timur Uun Yanuhar; Andik Isdianto; Defa Rizqi Machfuda; Gilang Rusrita Aida; Lutfia Fahimatul Ilmi; Nezya Pramudya Wardani; Nico Rahman Caesar
Samakia : Jurnal Ilmu Perikanan Vol 16 No 1 (2025): Samakia: Jurnal Ilmu Perikanan
Publisher : Faculty of Science and Technology University Ibrahimy

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35316/jsapi.v16i1.6058

Abstract

Pencemaran mikroplastik di ekosistem perairan telah menjadi perhatian global karena dampaknya terhadap biota laut, termasuk ikan yang merupakan sumber pangan utama bagi manusia. Penelitian ini bertujuan untuk mengkaji pengaruh cemaran mikroplastik terhadap profil darah ikan bandeng (Chanos chanos) di perairan utara Jawa Timur. Sampel ikan bandeng diambil dari beberapa lokasi lokasi yang memiliki tingkat pencemaran mikroplastik berbeda. Analisis mikroplastik dalam jaringan ikan dilakukan menggunakan metode mikroskopis dan spektroskopi, sementara analisis profil darah mencakup pemeriksaan parameter hematologis seperti jumlah eritrosit, leukosit, kadar hemoglobin, dan hematokrit. Hasil penelitian menunjukkan adanya akumulasi mikroplastik dalam jaringan ikan bandeng di seluruh lokasi pengambilan sampel, dengan konsentrasi tertinggi ditemukan di area dengan aktivitas industri dan pemukiman padat. Pengaruh mikroplastik terhadap profil darah ikan terlihat dari peningkatan jumlah leukosit dan penurunan kadar hemoglobin yang signifikan pada ikan yang terpapar mikroplastik dalam jumlah tinggi. Hal ini mengindikasikan adanya respon stres dan inflamasi akibat paparan mikroplastik, yang berpotensi memengaruhi kesehatan ikan dan kemampuan adaptasinya terhadap kondisi lingkungan. Penelitian ini menunjukkan bahwa cemaran mikroplastik dapat berdampak negatif pada sistem hematologi ikan bandeng, yang berimplikasi pada kesehatan ekosistem dan keamanan pangan bagi manusia. Temuan ini mendukung perlunya tindakan mitigasi pencemaran mikroplastik di perairan pesisir serta pengembangan kebijakan pengelolaan lingkungan yang lebih efektif.
Monitoring Kualitas Air pada Tambak Udang Vaname (Litopenaeus vannamei) di Perairan Pantai Utara Jawa Timur Nico Rahman Caesar; Uun Yanuhar; Andik Isdianto; Ekwan Nofa Wiratno; Lutfi Ni`'Matus Salamah; Zainal Abidin; Alfi Nur Rusydi; Umi Zakiyah; Hartati Kartikaningsih; Gilang Rusrita Aida; Lutfia Fahimatul Ilmi
Samakia : Jurnal Ilmu Perikanan Vol 16 No 1 (2025): Samakia: Jurnal Ilmu Perikanan
Publisher : Faculty of Science and Technology University Ibrahimy

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35316/jsapi.v16i1.6153

Abstract

Penelitian ini bertujuan untuk mengevaluasi kualitas perairan di Pantai Utara Jawa Timur berdasarkan parameter suhu, salinitas, pH, Total Dissolved Solids (TDS), Total Suspended Solids (TSS), oksigen terlarut (DO), amonia, nitrat, dan ortofosfat. Penentuan lokasi sampling dilakukan menggunakan metode purposive random sampling di tiga lokasi, yaitu Kabupaten Sidoarjo, Gresik, dan Lamongan. Hasil pengukuran menunjukkan suhu perairan berkisar antara 29,5–31,7 °C, sesuai dengan standar optimal untuk biota laut (28–32 °C). Salinitas dan pH (7,3–7,6) berada dalam batas wajar, mendukung kehidupan organisme akuatik. Nilai TDS di bawah 1000 ppm, sementara rata-rata TSS adalah 10,4 mg/L, jauh di bawah ambang batas 20–80 mg/L. Konsentrasi DO berkisar 4,7–6,97 mg/L, hampir memenuhi standar optimal > 5 mg/L. Kandungan amonia (0,007–0,088 mg/L) dan nitrat (0,051–0,095 mg/L) berada dalam batas aman. Konsentrasi ortofosfat (0,025–0,123 mg/L) melampaui ambang batas 0,015 mg/L di beberapa lokasi, kemungkinan disebabkan oleh limbah domestik. Hasil penelitian ini menunjukkan kondisi perairan Pantai Utara Jawa Timur mendukung ekosistem akuatik, meskipun potensi ancaman dari aktivitas manusia memerlukan pengelolaan lebih lanjut untuk menjaga keberlanjutan ekosistem
Effect of Extracellular Crude Protein (ECP) on expression IL-1, IL-6, IFN- in nile tilapia (Oreochromis niloticus) Cucun Herlina; Mohammad Fadjar; Maftuch Maftuch; Uun Yanuhar
Depik Vol 14, No 3 (2025): SEPTEMBER 2025
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.14.3.43338

Abstract

Modern strategies incorporate preventive measures such as extracellular products, as soluble proteins with antigenic properties, as well as generating a broad immune response. The aim of research is to determine and analyze the effect of the extracellular product of E. tarda on enhancing the immunity of Nile tilapia (O. niloticus). The research design with treatment durations of d0, 3rd, 6th, 9th day, and control (A, B, C, D, K). ECP vaccination was injected intraperitoneally at a dose of 0.1 ml/fish. The parameters analyzed are the expression of IL-1, IL-6, and IFN-, phagocytic activity, leukocyte differential, total white blood cells, and relative percent survival. The results of phagocytosis activity were highest in treatment C at 84.18% 1.81. The highest leukocyte differential in treatment C was lymphocytes at 56% 2.00, monocytes at 28% 2.00, and 16% 0.00, with a total leukocyte count of 29 x 10^3 cells 2.64, and RPS values ranging from 83.33% to 100% over 9 days of post-vaccination maintenance. Then, post-vaccination, there was an increase in the expression levels of the cytokine genes IL-1 and IL-6, but there was no increase in the expression levels of the IFN- gene compared to the control in Nile tilapia. (O. niloticus).Keywords:ECPIL-1IL-6IFN-Immune Response
Co-Authors Abdul Rahem Faqih Ach. Khumaidi Adelya Desi Kurniawati Adhitya, Dewa Sukma Trinanda Agus Susilo Alfarisi, Muhammad Asnin Alfi Nur Rusydi Amasda, Naufal Rizky Aminnudin, Aminnudin Amira Baihani Amira Masitha Anasiru, Arief Farhan Kurniawan Andi Kurniawan Andik Isdianto Anggraini, Rifka Rimbi Anisa Millah Taqiyyah Anjasmara, Aang Setyawan Annisa Fitri Anggreini Apri Supii Asep Awaludin Prihanto Asus Maizar Suryanto H Asus Maizar Suryanto Hertika Awalia Oktaviana Baihani, Amira Bili Darnanto Susilo BS, Sumitro Choirul Huda Choirul Huda Cucun Herlina Defa Rizqi Machfuda Diana Arfiati Dwi Retna Kumalaningrum Dyah Kinasih Wuragil Dyah Kinasih Wuragil Dyah Kinasih Wuragil Dyah Tri Rahayu Dyah Wuragil E Ernawati, E Eddy Suprayitno Edi Susilo Ekwan Nofa Wiratno Endang Yuli Herawati Fadjar, Mohammad Fajar Shodiq Fathah, Aulia Lanudia Fauliza, Tifa feri Setiawan Fika Fitrianesia Fitrianesia, Fika Gatot Ciptadi Gede Angga Krishna Fariestha Gian Suryanatha Hartawan Gian Suryanatha Hartawan Gilang Rusrita Aida Gilang Rusrita Aida Gunanti Mahasri Hardoko Hardoko Hartati Kartikaningsih Herlina, Cucun Herly Evanuarini Herly Evanuarini Herly Evanuarini Heru Suryanto Immaria Fransira Indah Wahyuni Abida Irawanto, Yovan Endik Islamy, R Adharyan Ismail, Mahrus Ita Fitria S Jati Batoro Jibril Maulana Kharisma Nuria Fitri Kristiani, Maria Lutfi Ni`'Matus Salamah Lutfia Fahimatul Ilmi MA, Widodo Maftuch ., Maftuch Maftuch Maftuch Maftuch Maftuch Maheno Sri Widodo, Maheno Sri Marsono Marsono Martiningsih, Nur Fauzi-yah Martiningsih, Nur Fauziyah Masitha, Amira Mohammad Bisri Mohammad Fadjar Mohammad Fadjar Mohammad Musa Mufidah, Rhobithotus Muhamad Muhajir Muhammad Faiq Ash Shiddiq Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Sumsanto Muhammad Sumsanto Musa, Mohammad Nezya Pramudya Wardani Nico Caesar Nico Rahman Caesar Nizery, Sefhanissa Puspa Retno Nur Fauziyah Martiningsih Nur Junirahma Nur Sakinah Junirahma Nusantara, Fajar Osman, Azlin Fazlina Pahlefi, Reval Pradana, Yanuar Rohmat Aji Purwoko, Agus Puspitasari, Intan Dwi Putri, Berlania Mahardika Ramadhanti, Nur Fa'izah Fitri Rifka Rimbi Anggraini Rika Wahyuningtyas Rika Wahyuningtyas Rika Wahyuningtyas, Rika Rizka Rahmana Putri Sanoesi, Ellana Sansi, Delfina Husniya Septi Anitasari, Septi Setyayunita, Tamaryska Shiddiq, Muhammad Faiq Ash Sias, Quota Alief Soelistyoadi, Rachmat Noer Soemarno Soemarno Sri Andayani Sri Andayani Sri Andayani Andayani Sri Rahmaningsih Sri Rahmaningsih Sukarni Sukarni Suprayitno Suprayitno Susilowati, Surya Rachman Taufik Budhi Pramono Taufik Budhi Pramono Teotonio Gomes, Guilherme Tri Djoko Lelono Umi Zakiyah Utari, Inpita Casuarina Eqisetia Wahyudi, Yusuf Arif Wardani, Nezya Pramudya Wuragil, Dyah Kinasih Yenny Risjani Yovan Endik Irawanto Yusuf Arif Wahyudi Yusuf Wahyudi Zainal Abidin