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All Journal Journal of Food and Pharmaceutical Science Vegetalika Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Bioma : Berkala Ilmiah Biologi Jurnal Ilmu Dasar AL KAUNIYAH AGRICOLA Indonesian Journal of Biotechnology Bioeksperimen: Jurnal Penelitian Biologi Journal of Tropical Biodiversity and Biotechnology Omni-Akuatika Jurnal Biologi Tropis Biosaintifika: Journal of Biology & Biology Education Journal of Multidisciplinary Applied Natural Science Proceeding Biology Education Conference Berkala Penelitian Hayati Asia Pacific Journal of Sustainable Agriculture, Food and Energy (APJSAFE) Journal of Food and Pharmaceutical Sciences

Yekti Asih Purwestri

Faculty Of Biology, Universitas Gadjah Mada

Author-ID : 422725

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Veterinary

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α-Amilase Inhibitory Activity of Fraction of Ethanolic Extract of Lebui Seed (Cajanus cajan (L.) Millsp.) Grown in West Nusa Tenggara Dian Resti Setyaningrum; Rumiyati; Yekti Asih Purwestri; Satrijo Saloko; Agung Endro Nugroho; Yudi Pranoto; Sri Widyastuti
Journal of Food and Pharmaceutical Sciences Vol 8, No 2 (2020): J. Food Pharm. Sci
Publisher : Institute for Halal Industry and System (IHIS) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.720

Diabetes is a metabolic syndrome characterized by high blood sugar levels or hyperglycemia.Alpha-amylase inhibitors can be used as an antidiabetic that controls postprandial hyperglycemia.Alpha-amylase inhibitors are found in plants as protein and non-protein bioactive content such as in lebuiseed. The purpose of this study was to determine the activity of alpha-amylase inhibition of the fraction ofethanol extract of lebui seed. This research was carried out by extraction of lebui seed and then fractionatedlebui extract to obtain n-hexane fraction (HF), ethyl acetate fraction (EAF), and water fraction (WF). Thefractions then tested to alpha-amylase inhibition activity in vitro by measuring the reducing sugar using3.5-dinitrosalicylic (DNS) reagent. Furthermore, the identification of the bioactive content of each fraction wascarried out using thin layer chromatography. The results of alpha-amylase inhibition activity test showed thatall fractions had the ability to inhibit alpha-amylase. The IC50 value of the n-hexane fraction, ethyl acetatefraction, and water fraction were 173.05 mg/ml, 9.98 mg/ml, and 137.19 mg/ml, respectively. Ethyl acetatefraction has the smallest IC50 value compared to other fractions. Based on the identification of the bioactivecontent, bioactive content that is only found in the ethyl acetate fraction but not in other fractions are tannins.Therefore, the chemical content in lebui seed which acts as an alpha-amylase inhibitor might be tannins.

Deteksi Gen Ketahanan Hawar Daun Bakteri Xa21 pada Padi (Oryza sativa L.) Hitam dan Merah Lokal Indonesia Andi Setiawan; Alfino Sebastian; Yekti Asih Purwestri
Vegetalika Vol 10, No 2 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.36625

Beras berpigmen mulai popular dikonsumsi oleh masyarakat sebagai bahan pangan fungsional. Tetapi, terdapat faktor pembatas produksi beras berpigmen yaitu penyakit hawar daun bakteri yang disebabkan oleh Xanthomonas oryzae pv. Oryzae (Xoo). Penggunaan varietas tahan yang memiliki gen ketahanan Xa dinilai efektif untuk menanggulangi masalah penurunan hasil padi. Gen Xa ini antara lain terdiri dari gen Xa21. Penelitian ini bertujuan untuk mengetahui keberadaan gen ketahanan hawar daun bakteri Xa21 pada padi hitam kultivar Sembada Hitam, Cempo Ireng, Melik dan Hitam Toraja serta padi merah kultivar Aek sibondang, Merah Sumbawa, Segreng, dan Pari Eja di Indonesia. Metode penelitian meliputi isolasi genom padi, pengecekan hasil isolasi DNA dengan elektroforesis gel agarosa (0,8%), pengukuran konsentrasi dan kemurnian DNA, amplifikasi DNA primer pTA248, dan analisis data. Hasil penelitian ini mendeteksi keberadaan gen Xa21 pada semua kultivar padi hitam dan merah tersebut dengan dengan sifat tahan.

Optimasi Metode 1H-NMR Profiling pada Rimpang Kunyit (Curcuma domestica) Caroline Dwiseptianti; Febri Adi Susanto; Yekti Asih Purwestri; Tri Rini Nuringtyas
Bioeksperimen: Jurnal Penelitian Biologi Vol 5, No 2: September 2019
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v5i2.9238

The use of medicinal plants is increasing due to the lack of side effects caused and the number of bioactive compounds that cannot be represented by synthetic chemical synthesis compounds. However, the management and use of natural medicines for the main handling of diseases are often hampered by the quality of the ingredients which are low and unstable. The standardized quality control system of OAI (Indonesian Natural Medicine) is the main key to improve clinical assurance and safety of the use of herbal medicines in Indonesia. One of the medicinal plants known to the public is Curcuma longa L. (turmeric). The main active components contained in turmeric are curcumin, demetoksikurkumin, bis-demetoksikurkumin, and ar-turmeron. Information about the quality of turmeric is needed in its use as a raw material for drugs so we need an analytical technique that is able to identify the diversity of metabolite profiles of active compounds. In this research, an optimization method is used to improve efficiency in the extraction of turmeric rhizome metabolites so that the best solvent concentration is known for the analysis of fingerprinting secondary metabolites with 1H-NMR 500 MHz spectroscopy in turmeric rhizomes. The results were analyzed with MNOVA software and chemical shift obtained compared with the reference. From the results obtained a concentration of methanol-d4 (CD3OD) 100% able to extract curcumin better than other solvents. The solvent is able to extract saccharide (sugar) compounds in the form of sucrose, amino acids and fatty acids in the form of methionine, glutamine, acetate, and glycero phospho choline.

Morphological, Histological, and Protein Profiling of Tea Embryo Axis at Early Stage of Culture Ratna Dewi Eskundari; Taryono Taryono; Didik Indradewa; Yekti Asih Purwestri
Journal of Tropical Biodiversity and Biotechnology Vol 6, No 3 (2021): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.64403

Tissue culture is an alternative choice of plant propagation either through somatic embryogenesis or in vitro organogenesis techniques. TRI2025 tea clone has been cultured successfully, however, the scientific information related to morphology, histology, and protein profile at an early event of culturing time has not been reported yet. This study aimed to determine the differences between those pathways, in the context of morphology, histology, and protein profile. The explants were the embryo axis of TRI2025 tea clone cultured on two different induction mediums; somatic embryogenesis and in vitro organogenesis induction medium. The results showed that most of the explants cultured on A medium developed to be a globular-like structure at 11-day after culture (DAC), while all explants cultured on B medium showed the initiation stage of in vitro organogenesis. Histological analysis showed meristem reconstruction at shoot apical meristem (SAM) and root apical meristem (RAM) at 11-DAC at explants cultured on B medium, while explants cultured on A medium showed callusing at 21-DAC. Protein profile analysis using SDS-PAGE showed protein bands of 54 and 81 KDa that only appeared at explants cultured on A medium start from 14-DAC, and those two protein bands thought to be a differentiator at the early stages of the two tissue culture techniques. Thus, these parameters can be used as early detection for plant tissue culture, especially in tea.

Potensi Bakteri Endofit Asal Tanaman Pisang Klutuk (Musa balbisiana Colla) Sebagai Pendukung Pertumbuhan Tanaman Triastuti Rahayu; Yekti Asih Purwestri; Siti Subandiyah; Donny Widianto
Al-Kauniyah: Jurnal Biologi Vol 14, No 2 (2021): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v14i2.19140

AbstrakBakteri endofit yang terdapat di tanaman pisang Klutuk dan keterkaitannya dengan sifat ketahanan tanaman pisang Klutuk pada cekaman biotik dan abiotik belum dilaporkan dalam publikasi ilmiah. Sebanyak 93 isolat bakteri endofit telah diperoleh dari pisang Klutuk, tetapi belum diketahui kemampuannya sebagai pendukung pertumbuhan tanaman (PPT). Penelitian ini bertujuan untuk mengetahui karakter isolat-isolat bakteri endofit dari pisang Klutuk sebagai pendukung pertumbuhan tanaman. Kelompok bakteri Gram positif dan negatif ditentukan dengan metode pewarnaan Gram. Kemampuan memfiksasi nitrogen (N2), memproduksi asam indol asetat (AIA), dan antagonisme terhadap Fusarium oxysporum f. sp. cubense (Foc) diuji untuk mengetahui kemampuan isolat bakteri endofit sebagai pendukung pertumbuhan tanaman. Hasil penelitian menunjukkan bahwa 87,10% isolat bakteri endofit dari tanaman pisang Klutuk merupakan kelompok bakteri Gram negatif dan 82,80% (77 isolat bakteri) menunjukkan karakter tunggal atau ganda sebagai PPT. Di dalam kelompok isolat tersebut, terdapat berturut-turut 60, 38, dan 20 bakteri yang mampu memfiksasi N2, menghasilkan AIA, dan antagonisme terhadap Foc. Hasil pengujian ini menunjukkan bahwa bakteri endofit dari pisang Klutuk didominasi oleh bakteri kelompok Gram negatif yang memiliki kemampuan sebagai pendukung pertumbuhan tanaman.Abstract The role of endophytic bacteria on the biotic and abiotic resistance of Klutuk banana plants has never been reported. A total of 93 endophytic bacterial isolates were obtained from Klutuk banana plants in a previous study, but their potency as Plant Growth Promoting Bacteria (PGPB) is not elucidated. This study aims to characterize those 93 endophytic bacterial isolates. Gram staining was performed to differentiate between Gram-positive and negative bacteria among the isolates. The ability to fix nitrogen (N2), produce indole acetic acid (IAA) and antagonize Fusarium oxysporum f. sp. cubense (Foc) were also examined to determine their potency as PGPB. The results showed that 87.1% of the endophytic bacterial isolates were Gram-negative bacteria and 83.87% (78 bacterial isolates) had single or multiple traits of PGPB. Among the isolates, 60, 38, and 20 bacteria were able to fix N2, produce IAA, and antagonize Foc, respectively. The results indicated that the endophytic bacteria inhabiting Klutuk banana plant are dominated by Gram-negative PGPB.

Identifikasi Senyawa Curcumin, Bisdemethoxycurcumin dan Demethoxycurcumin Rimpang Curcuma xanthorrhiza Roxb. dan Curcuma longa L. dengan 1H NMR Erwin Nur Indiarto; Tri Rini Nuringtyas; Yekti Asih Purwestri; Respati Tri Swasono; Yosi Bayu Murti
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 15, No 1 (2018): Proceeding Biology Education Conference
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar

Curcumin and its derivates (Bisdemethoxycurcumin, Demethoxycurcumin) are natural dyes found in Curcuma xanthorrhiza dan Curcuma longa. Parmacologic evidence suggests all three are antioxidant, antiinflamatory, and anticancer. The objective of this research was to identify and measure the intensity of Curcumin compounds, Bisdemethoxycurcumin, and Demethoxycurcumin rhizome C. xanthorrhiza and C. longa by using 1H NMR spectra. Rhizome C.xanthorrhiza and C. longa collected from Wonogiri, Central Java. The rhizomes were dried in the oven at 40 °C and were powdered using a blender. The dried powder sample was extracted using methanol-d4 containing 0.01% TMSP and followed by ultrasonication for 15 minutes. The supernatant was separated from the solution by 10.000 rpm centrifugation for 10 minutes. The supernatant was transferred to NMR tube and analysed by using JEOL 500 MHz NMR. The obtained data were analysed by Mnova 12 software and Simca 14. Results of comparsion of 1H NMR spectra of both rhizomes with reference were identified Curcumin, Bisdemethoxycurcumin, Demethoxycurcumin compounds. The highest intensity all of three compound was found in Curcuma longa and with One way Anova test result obtained p<0,05. The two rhizomes show a good separation on the OPLS-DA scoreplot with Q2 82,1% indicating a good model. The study showed that Curcumin, Bisdemethoxycurcumin, Demethoxycurcumin compounds were found in both rhizomes and had different intensity

Developmental the pattern of embryogenic microspore of rice (Oryza sativa L.) based on morphological characteristic Siti Nurbaiti; Yekti Asih Purwestri; Budi Setiadi Daryono; Endang Semiarti; Ari Indrianto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 25 No 1 (2019): December 2019
Publisher : The East Java Biological Society

Microspore culture is a rapid approach to get double haploid plants in shorten time which is important for plant breeding. Microspore culture of pigmented rice cultivar Segreng was used to observe the pattern of embryogenic development based on the characteristics of morphology. Characterization between embryogenic and non-embryogenic microspores was conducted after exposed anther to carbohydrate starvation in B medium culture at 33oC for 4 days. The result showed that enlarged microspore and star-like structure of fragmented cytoplasm become a marker of embryogenic microspore after stresses treatment. More complex of fragmented microspore was found at 4 days in A2 medium and developed further into multicellular structure. These multicellular structures might be originated either by symmetrical or asymmetrical division. The longer incubation, the higher frequency of complex fragmented microspore that developed into multicellular structure release from exine has appeared.

Characterization of Lignocellulolytic Bacteria from Gut of Termite (Isoptera: Rhinotermitidae and Termitidae) Putri Dwi Mulyani; Muhammad Rizky Ulil Albab; Yekti Asih Purwestri
Jurnal Biologi Tropis Vol. 21 No. 2 (2021): Mei - Agustus
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v21i2.2737

A total of 10 bacterial isolates have been isolated from the gut of termites (Isoptera: Rhinotermitidae and Termitidae) and are known to have the ability to produce lignocellulolytic enzymes consisting of cellulase, laccase, and lignin peroxidase. The enzymatic ability allows these bacteria to be used as a source of new enzymes in the industrial world. However, further research on the character of bacteria to support identification has not been carried out. This study aims to characterize lignocellulolytic bacteria in the gut of termites morphologically and biochemically. Morphological observations were carried out including colony shape, colony edge, colony color, growth type, bacterial cell shape, and gram staining. Meanwhile, the biochemical characterization carried out included glucose fermentation test, indole formation, starch hydrolysis, catalase test, and nitrate reduction. The results showed that ten isolates of lignocellulolytic bacteria from the gut of termites (Isoptera: Rhinotermitidae and Termitidae) grew facultatively anaerobic, had almost similar morphological characters, with colony shapes including irregular and filamentous, colony edges in the form of lobate, undulate, and filamentous, and coloration. Colonies are white to yellowish white. The result of gram staining showed that most of the bacteria were gram positive bacteria with coccus and bacillus shaped bacterial cells. Biochemical analysis showed that these bacteria have the ability to ferment glucose, hydrolyze starch, reduce hydrogen peroxide (HÃ¢ââOÃ¢ââ 30%) and reduce nitrate.ÃÂ

Optimization of heat shock temperature and time on the transformation of pRGEB32 into Escherichia coli DH5ÃÂ± Rosy Feraningsih Patigu; Putri Wijayanti; Alfino Sebastian; Yekti Asih Purwestri
Jurnal Biologi Tropis Vol. 21 No. 3 (2021): September - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v21i3.2811

Genome editing technique is one of the methods for studying the expression of gene, eliminating unfavorable traits or phenotypes and generating the new characters of species. The pRGEB32 plasmid is one of the vectors that used in genome editing with carrying the Cas9 gene, restriction site of sgRNA (single guide RNA) and specific promoters that can be expressed in plants. The first step in the genome editing process is inserting pRGEB32 into Escherichia coli for propagation. The large size of the plasmid molecule becomes a challenge to determine the right method in the transformation process. This study aims to determine the temperature and time of heat shock transformation of plasmid pRGEB32 into E. coli. The transformation of pRGEB32 into plasmids was carried out with variations in temperature and time, 42Ã¢âÆ (30 seconds and 60 seconds) and 55Ã¢âÆ (30 seconds and 60 seconds). The results showed that a heat shock temperature of 55Ã¢âÆ with a time of 60 seconds was the best temperature for the transformation of pRGEB32 into E. coli. This optimization of heat shock condition will increase the transformation efficiency, which is in the range of 3322-10.989 cfu/ÃÂµg.ÃÂ

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.720

Co-Authors Abdul Rahman Siregar, Abdul Rahman Adania, Baik Aisyah Agung Endro Nugroho Alfino Sebastian Alfino Sebastian Alfino Sebastian Almunawar, Muhammad Fikri Andi Setiawan Anis Uswatun Khasanah Anjar Tri Wibowo Aprilianti, Suci Hari Ardaning Nuriliani Ari Indrianto Arnia Sari Mukaromah Bassalamah, Rizka Fahma Budi Setiadi Daryono Cahyo Wulandari Caroline Dwiseptianti Charlie Ester de Fretes Conara, Flafiani Cios Diah Rachmawati Dian Resti Setyaningrum Didik Indradewa Didik Indradewa Didik Indradewa Dio N. Wijaya Donny Widianto Donny Widianto Dwiseptianti, Caroline Dyah Ismoyowati, Dyah Ekowati, Nurhening Yuni Ekris Sutiyanti Elvani, I Sabila Endang Semiarti Erni Martani Erni Martani Erwin Nur Indiarto Fajar Sofyantoro Febri Adi Susanto Febri Adi Susanto Ghea Putri Christy Hidayah, Lailly Tsania Nur Hidayat, Rheina Faticha Asyamsa Himawan, Tyas Ikhsan Hiroyuki Tsuji Hiroyuki Tsuji, Hiroyuki Ikhsan Maulana Khasanah, Novita Uswatun Ko Shimamoto Ko Shimamoto, Ko Kumala Dewi Kumala Dewi Langkah Sembiring LANGKAH SEMBIRING Langkah Sembiring Latifah, Emi Lila Natasaputra Lila Natasaputra, Lila Lisna Hidayati, Lisna Maharesi, Chesa Ekani Masashi Kawaichi Mataram, Made Bagus Auriva Muhammad Rizky Ulil Albab Mulyani, Putri Dwi Nasution, Nurlita Putri Bela Nugrahapraja, Husna Nurhidayah, Septika Oktavya, Galuh P. Parmi Parmi P, Parmi Pratiwi Apridamayanti Pratiwi, Apriliana Priyono, Dwi Sendi Putri Dwi Mulyani Putri Dwi Mulyani Putri Wijayanti Putri, Wahyu Aristyaning Rachman, Mifta Pratiwi Radhiyah Mardhiyah Hamid Radhiyah Mardhiyah Hamid Rahayu, Hanum Mukti Rarastoeti Pratiwi Rarastoeti Pratiwi Ratna Dewi Eskundari Ratna Stia Dewi Ratna Stia Dewi Respati Tri Swasono Retnowati, Faizah Diah Rifqi Zahroh Janatunaim Rifqi Zahroh Janatunaim Rina Sri Kasiamdari Rosy Feraningsih Patigu Rumiyati Rumiyati Rumiyati Saragih, Hendry T.S.S.G Satrijo Saloko Sebastian, Alfino Sine, Yuni Siti Nurbaiti Siti Subandiyah Sofyana, Neng Tanty Sri Widyastuti Sri Widyastuti Subiastuti, Aprilia Sufi Susanto, Febri Adi Syam, Adi Mazdi Taryono Taryono Taryono, Taryono Tri Rini Nuringtyas Tri Rini Nuringtyas Triastuti Rahayu Triyaningsih Triyaningsih Triyaningsih, Triyaningsih Wahyu Aristyaning Putri Woro Anindito Sri Tunjung Yohana Theresia Maria Astuti Yohana Theresia Maria Astuti, Yohana Theresia Maria Yoshiharu Fujii Yosi Bayu Murti Yudi Pranoto Yuka Ogaki Yuka Ogaki, Yuka Yustina Carolina Febrianti Salsinha

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