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All Journal Journal of Food and Pharmaceutical Science Vegetalika Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Bioma : Berkala Ilmiah Biologi Jurnal Ilmu Dasar AL KAUNIYAH AGRICOLA Indonesian Journal of Biotechnology Bioeksperimen: Jurnal Penelitian Biologi Journal of Tropical Biodiversity and Biotechnology Omni-Akuatika Jurnal Biologi Tropis Biosaintifika: Journal of Biology & Biology Education Journal of Multidisciplinary Applied Natural Science Proceeding Biology Education Conference Berkala Penelitian Hayati Asia Pacific Journal of Sustainable Agriculture, Food and Energy (APJSAFE) Journal of Food and Pharmaceutical Sciences
Yekti Asih Purwestri
Faculty Of Biology, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Veterinary

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Ethanolic Extract of Black Rice ‘Sembada Hitam’ Bran Did not Show Cytotoxic Effect on HeLa Cell (Cervical Cancer Cell Line) Nuriliani, Ardaning; Conara, Flafiani Cios; Oktavya, Galuh; Hidayah, Lailly Tsania Nur; Purwestri, Yekti Asih
Bioma : Berkala Ilmiah Biologi Vol. 25, No 1, Tahun 2023
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.25.1.49-59

Abstract

Black rice (Oryza sativa L.) contains various phenolic compounds such as anthocyanins which act as antioxidants. Antioxidants inhibit cell proliferation and induced apoptosis of cancer cells as well as protect cells from damage that triggers cancer. 'Sembada Hitam' is a new cultivar of black rice in Indonesia that has not been studied for its anticancer potential. This study aimed to study the cytotoxic activity and apoptosis induction of the ethanolic extract of black rice (Oryza sativa L. 'Sembada Hitam') bran against HeLa cells. The method used was the cytotoxicity test with the MTT assay and the apoptosis test with the AO-PI double staining method. The test used various concentrations of black rice bran extract, DMSO as solvent control, and doxorubicin as a positive control for 24 and 48 hours. Data analysis was performed using one-way ANOVA (p = 0.05) followed by the Tukey HSD test. The results showed that ethanolic extract of black rice 'Sembada Hitam' bran has no cytotoxic activity against HeLa cells (p > 0.05). Moreover, extracts at the concentrations of 250 and 500 µg/mL only induced apoptosis of HeLa cells by 11.62 ± 0.50 and 11.49 ± 9.88% respectively. These results indicate that the ethanolic extract of black rice 'Sembada Hitam' bran has no potential to be developed as an agent for cervical cancer therapy.
Optimasi Metode 1H-NMR Profiling pada Rimpang Kunyit (Curcuma domestica) Dwiseptianti, Caroline; Susanto, Febri Adi; Purwestri, Yekti Asih; Nuringtyas, Tri Rini
Bioeksperimen: Jurnal Penelitian Biologi Vol 5, No 2: September 2019
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v5i2.9238

Abstract

The use of medicinal plants is increasing due to the lack of side effects caused and the number of bioactive compounds that cannot be represented by synthetic chemical synthesis compounds. However, the management and use of natural medicines for the main handling of diseases are often hampered by the quality of the ingredients which are low and unstable. The standardized quality control system of OAI (Indonesian Natural Medicine) is the main key to improve clinical assurance and safety of the use of herbal medicines in Indonesia. One of the medicinal plants known to the public is Curcuma longa L. (turmeric). The main active components contained in turmeric are curcumin, demetoksikurkumin, bis-demetoksikurkumin, and ar-turmeron. Information about the quality of turmeric is needed in its use as a raw material for drugs so we need an analytical technique that is able to identify the diversity of metabolite profiles of active compounds. In this research, an optimization method is used to improve efficiency in the extraction of turmeric rhizome metabolites so that the best solvent concentration is known for the analysis of fingerprinting secondary metabolites with 1H-NMR 500 MHz spectroscopy in turmeric rhizomes. The results were analyzed with MNOVA software and chemical shift obtained compared with the reference. From the results obtained a concentration of methanol-d4 (CD3OD) 100% able to extract curcumin better than other solvents. The solvent is able to extract saccharide (sugar) compounds in the form of sucrose, amino acids and fatty acids in the form of methionine, glutamine, acetate, and glycero phospho choline.
Orchid‐associated endophytic Bacillus mediates Fusarium suppression and promotes in vitro regeneration of banana plantlets via culture supernatant Sasongko, Aries Bagus; Purwestri, Yekti Asih; Daryono, Budi Setiadi; Subandiyah, Siti
Indonesian Journal of Biotechnology Vol 30, No 4 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.111727

Abstract

The application of Fusarium‐antagonistic endophytic bacteria with plant growth‐promoting traits offers an effective method to enhance the success of banana plantlet tissue culture while combating Fusarium wilt disease caused by Fusarium oxysporum f.sp. cubense Tropical Race 4 (FocTR4) (VCG 01213). This study evaluates the endophytic bacterium AP3311, isolated from healthy banana roots in direct association with orchid roots. AP3311 exhibited strong antagonism toward FocTR4, hyphal colonization ability, and multiple growth‐promoting activities, including phosphate solubilization, nitrogen fixation and auxin production. 16S rRNA gene sequencing identified that AP3311 belongs to the genus Bacillus, while metabarcoding analysis revealed that Bacillus species dominate the root microbiomes of both bananas and orchids. The bacterial supernatants stimulated root development and leaf growth in vitro. Metabolomic profiling indicated that antimicrobial compounds, together with plant growth regulators, promoted both root and shoot growth. Overall, the research demonstrates that Bacillus sp. AP3311 and its supernatants are valuable components in banana tissue culture, providing the dual benefits of plant growth promotion and effective disease control.
Co-Authors Abdul Rahman Siregar, Abdul Rahman Adania, Baik Aisyah Agung Endro Nugroho Alfino Sebastian Alfino Sebastian Alfino Sebastian Almunawar, Muhammad Fikri Andi Setiawan Anis Uswatun Khasanah Anjar Tri Wibowo Aprilianti, Suci Hari Ardaning Nuriliani Ari Indrianto Aries Bagus Sasongko, Aries Bagus Arnia Sari Mukaromah Bassalamah, Rizka Fahma Budi Setiadi Daryono BUDI SETIADI DARYONO Cahyo Wulandari Caroline Dwiseptianti Charlie Ester de Fretes Conara, Flafiani Cios Diah Rachmawati Dian Resti Setyaningrum Didik Indradewa Didik Indradewa Didik Indradewa Dio N. Wijaya Donny Widianto Donny Widianto Dwiseptianti, Caroline Dyah Ismoyowati, Dyah Ekowati, Nurhening Yuni Ekris Sutiyanti Elvani, I Sabila Endang Semiarti Erni Martani Erni Martani Erwin Nur Indiarto Fajar Sofyantoro Febri Adi Susanto Febri Adi Susanto Ghea Putri Christy Hidayah, Lailly Tsania Nur Hidayat, Rheina Faticha Asyamsa Himawan, Tyas Ikhsan Hiroyuki Tsuji Hiroyuki Tsuji, Hiroyuki Ikhsan Maulana Khasanah, Novita Uswatun Ko Shimamoto Ko Shimamoto, Ko Kumala Dewi Kumala Dewi Langkah Sembiring Langkah Sembiring LANGKAH SEMBIRING Latifah, Emi Lila Natasaputra Lila Natasaputra, Lila Lisna Hidayati, Lisna Maharesi, Chesa Ekani Masashi Kawaichi Mataram, Made Bagus Auriva Muhammad Rizky Ulil Albab Nasution, Nurlita Putri Bela Nugrahapraja, Husna Nurhidayah, Septika Oktavya, Galuh P. Parmi Parmi P, Parmi Pratiwi Apridamayanti Pratiwi, Apriliana Priyono, Dwi Sendi Putri Dwi Mulyani Putri Wijayanti Putri, Wahyu Aristyaning Rachman, Mifta Pratiwi Radhiyah Mardhiyah Hamid Radhiyah Mardhiyah Hamid Rahayu, Hanum Mukti Rarastoeti Pratiwi Rarastoeti Pratiwi Ratna Dewi Eskundari Ratna Stia Dewi Ratna Stia Dewi Respati Tri Swasono Retnowati, Faizah Diah Rifqi Zahroh Janatunaim Rifqi Zahroh Janatunaim Rina Sri Kasiamdari Rosy Feraningsih Patigu Rumiyati Rumiyati Rumiyati Saragih, Hendry T.S.S.G Satrijo Saloko Sebastian, Alfino Sine, Yuni Siti Nurbaiti Siti Subandiyah Sofyana, Neng Tanty Sri Widyastuti Sri Widyastuti Subiastuti, Aprilia Sufi Susanto, Febri Adi Syam, Adi Mazdi Taryono Taryono Taryono, Taryono Tri Rini Nuringtyas Tri Rini Nuringtyas Triastuti Rahayu Triyaningsih Triyaningsih Triyaningsih, Triyaningsih Wahyu Aristyaning Putri Woro Anindito Sri Tunjung Yohana Theresia Maria Astuti Yohana Theresia Maria Astuti, Yohana Theresia Maria Yoshiharu Fujii Yosi Bayu Murti Yudi Pranoto Yuka Ogaki Yuka Ogaki, Yuka Yustina Carolina Febrianti Salsinha