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The Effect of Red Ginger Extract (Zingiber officinale var rubrum) on The Thickness of The Epitelial and Diameter of Seminiferous Tubules in Albino Rat (Rattus norvegicus) Exposed Monosodium Glutamat Renaldi, Nogi; Rachmawati, Kadek; Widiyatno, Thomas Valentinus; Madyawati, Sri Pantja; Dhamayanti, Yeni
Journal of Basic Medical Veterinary Vol. 13 No. 1 (2024): Journal of Basic Medical Veterinary, June 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v13i1.51158

Abstract

Background: This study examined administration of MSG intraperitoneally to white rats resulted in a decrease in testicular weight, an increase in lipid peroxidase levels The testes, as the site of spermatogenesis, are highly vulnerable to oxidation processes caused by free  radicals,  which  can  disrupt spermatogenesis. Free radicals can be countered by antioxidants. One plant believed to have antioxidant activity is red ginger. Purpose: To determine the effect of red ginger against histopathology seminiferous tubules of white rat (Rattus norvegicus) exposed by monosodium glutamate. Methods: This study used 30 rats which were divided into 5 groups. The positve control group (K+) were given only monosodium glutamate 5g/kgBW and CMC Na orally, the treatments groups P1 were given monosodium glutamte 5g/KgBW + red ginger 50mg/rat/day, P2 were given 5g/KgBW + red ginger 100mg/rat/day, and P3 were given 5g/KgBW + red ginger 200mg/rat/day. All treatments were administered for 42 days. After 42 days of treatments, all rats were sacrificed and seminiferous tubules were observed with microscope from pathology laboratory. Results: Administration of red ginger extract can defend seminiferous tubules epithelial thicknes and diameter from exposure of monosodium glutamate with 100mg/KgBW dosage as the most effective dosage. Seminiferous tubules epitelial thickness and diameter were tested using one-way ANOVA and Duncan's multiple range test. Conclusion: Red Ginger Extract (Zingiber officinale var rubrum) has shown potential effects to counteract Monosodium Glutamat consequences on The Thickness of the epitelial and diameter of seminiferous tubules of white rat (Rattus norvegicus).
STEM CELL THERAPY ON ANIMAL MODEL (Rattus norvegicus) END GESTATIONAL TIME EXPOSED TO CARBON BLACK AS OBSERVED FROM CASPASE-3 EXPRESSION Achmad, Agung Budianto; Madyawati, Sri Pantja; Widjiati, Widjiati
Journal of Vocational Health Studies Vol. 1 No. 3 (2018): March 2018 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V1.I3.2018.102-106

Abstract

Background: Air pollution in the form of Diesel Exhaust Particles emerging from motor vehicles are harmful to health that have adverse reproductive health impacts, especially during pregnancy. The use of stem cells in treating white mice (Rattus norvegicus) exposed to carbon black can reveal the potential for treatment of placental impairment during pregnancy. Purpose: to demonstrate the effectivity of Rat Bone Marrow Mesenchymal Stem Cell therapy on rats (Rattus norvegicus) exposed to carbon black as observed from caspase-3 expression. Method: This research uses a completely randomized design with factorial pattern. Forty-eight gravid female rats were divided into six treatment groups. Result: caspase-3 expression in each treatment showed no significant differences in the groups treated with RBMMSC in each gravid groups treatment (therapy GD 11 and GD 17) were exposed to carbon black and not treated with RBMMSC. The same are indicated by the normal trophoblast cells (cytotrophoblast and syncytiotrophoblast cells) in the RBMMSC treated group showed no significant difference with the group exposed to carbon black only. Conclusion: this research indicate that Rat Bone Marrow Mesenchymal Stem Cell therapy in Rattus norvegicus exposed to carbon black have not been able to reduce expression of caspase-3.
Development of an Online Livestock Showroom to Enhance the Competitiveness of Local Farmers In Blora Madyawati, Sri Pantja; Mustofa, Imam; Srianto, Pudji; Saifudin, Ali
Jurnal Pengabdian Masyarakat Charitas Vol. 5 No. 01 (2025): Jurnal Pengabdian Masyarakat Charitas
Publisher : Program Studi Teknik Industri, Fakultas Teknik, Universitas Katolik Indonesia Atma Jaya Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25170/charitas.v5i01.6567

Abstract

The online livestock showroom development program aims to cut the long livestock distribution chain and create a more objective pricing system, especially for farmers in Blora Regency. The main partners of this activity are local farmers who are members of the Farmer Groups and Blora Livestock Community. The implementation method of the program involves training, mentoring, and collaboration with the government and the farming community. The results show that the showroomternak.id platform successfully improved marketing efficiency, expanded market access, and provided a more transparent pricing system based on morphometric data. As many as 87% of farmers responded positively to this application, and most were satisfied with the ease and fairness in pricing. However, we must still to address challenges like limited internet access and low technological skills. The recommendations from this program are the expansion of training, the improvement of digital infrastructure, and ongoing support for farmers to maximize their use of technology in the long term.
EMPOWERMENT OF DAIRY CATTLE FARMERS IN THE AREAS SELINGKAR WILIS THROUGH COMMUNITY SERVICE’S POSTGRADUATE SCHOOL: PEMBERDAYAAN PETERNAK SAPI PERAH DI SELINGKAR WILIS, PENGABDIAN KEPADA MASYARAKAT SEKOLAH PASCASARJANA Yuliani, M. Gandul Atik; Madyawati, Sri Pantja; Hariyono, Hariyono
Darmabakti Cendekia: Journal of Community Service and Engagements Vol. 7 No. 1 (2025): JUNE 2025
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/dc.V7.I1.2025.105-110

Abstract

Background: The Postgraduate School strives to establish itself as a world-class institution with a significant global impact. To achieve this vision, it has forged partnerships with regional leaders, emphasizing community service. Our latest initiative, "Community Empowerment of Dairy Cattle Breeders in Selingkar Wilis, Trenggalek Regency," aligns with the Sustainable Development Goals (SDGs). Objective: This initiative aims to empower the community in the Wilis region by providing training in dairy cattle reproduction and production. Method: The program is implemented in two key stages: (1) Offering guidance and training to dairy farmers, and (2) Monitoring and evaluating the outcomes. We measure knowledge gains through assessments conducted before and after the training sessions. Results: Evaluations indicated a significant improvement in dairy farmers' understanding of reproductive health management and the control of Foot and Mouth Disease in their cattle. Conclusion: This initiative has successfully enhanced the skills of breeders in the Wilis area, equipping them to address reproductive issues and manage Foot and Mouth Disease, thereby supporting the agricultural community in Trenggalek Regency.
Post-eruption economic recovery: Strengthening livelihoods in Lumajang Indonesia after Mount Semeru disaster Hariyono, Hariyono; Purwono, Rudi; Sukartini, Ni Made; Madyawati, Sri Pantja; Chrisnahutama, Adrian
Central Community Development Journal Vol. 5 No. 1 (2025): June 2025
Publisher : Privietlab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55942/ccdj.v5i1.351

Abstract

Natural disasters in Indonesia result in significant material and nonmaterial losses. According to the National Disaster Management Agency (BNPB), disasters in 2021 have caused 709 deaths, 73 missing persons, and displaced 583,840 people. Post-disaster recovery efforts, including economic assistance, are essential for restoring people’s livelihoods. The implementation of economic assistance after the Mount Semeru eruption in the Lumajang Regency included several stages: preparation, socialization and location survey, group formation, technical guidance, provision of stimulant assistance, exit strategy planning, and monitoring and evaluation. As a result, two livestock groups were established in the Bumi Semeru Damai permanent housing area, each consisting of 10 members and legally recognized by a village decree. These groups successfully carried out daily livestock management, enhanced productivity and welfare, improved market access, and increased the understanding of livestock product marketing. The initiative fostered sustainability and independence, with the groups evolving into leading livestock centers specializing in goats in the Lumajang Regency. Critical factors supporting sustainability included a sufficient supply of animal feed that met nutritional requirements. This economic assistance program played a vital role in revitalizing the local economy by utilizing local commodities, forming community-based economic institutions, and strengthening local capacities through a disaster risk reduction approach. In addition, the program secured local government and stakeholder support, contributing to long-term recovery and alignment with sustainable regional development. This case highlights the importance of integrated economic recovery programs in post-disaster contexts for building resilience and improving community welfare.
Detection of Anti-Müllerian Hormone (AMH) mRNA in Serum and Ovarian Tissue of Local Indonesian Cattle Using EvaGreen-based RT–qPCR Dikman, Dicky Mohammad; Puspitasari, Heni; Waluyo, Seagames; Kurniawan, Muhammad ‘Ahdi; Supriyadi, Supriyadi; Srianto, Pudji; Luqman, Epy Muhammad; Suprayogi, Tri Wahyu; Madyawati, Sri Pantja; Safitri, Erma; Lestari, Tita Damayanti
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.382-397

Abstract

Anti-Müllerian hormone (AMH) has been identified as a potential biomarker for assessing ovarian reserve and reproductive capacity in cattle, with high heritability and relatively stable expression. However, studies detecting AMH mRNA in bovine serum remain scarce due to the matrix’s inherently low RNA yield, susceptibility to degradation, and potential presence of PCR inhibitors. This study provides an exploratory validation of AMH mRNA detection in serum — a challenging matrix compared with ovarian tissue — using EvaGreen-based reverse transcriptase–quantitative PCR (RT–qPCR). Specific primers for the AMH and β-actin genes were designed in silico and validated through melting curve analysis and linearity testing. The results showed high amplification efficiency (AMH: 100.2%, R² = 0.994; β-actin: 109.1%, R² = 0.996), with specific amplification of both targets. AMH detection in serum samples was successful in some samples, while the β-actin gene was consistently amplified as a reference gene. Despite the low RNA quality from serum and the presence of organic contaminants, the method demonstrated its feasibility for detecting AMH transcripts in a minimally invasive manner. Physiologically, AMH levels positively correlate with antral follicle count, superovulation success, and embryo quality, and are sensitive to heat stress and other environmental factors. These findings provide a foundational basis for developing molecular diagnostic approaches based on AMH gene expression in cattle reproductive management programs and support the future development of efficient, accurate, and context-specific biomolecular-assisted selection technologies for tropical livestock systems.
Telemonitoring-Based Cardiac Care to Reduce Readmissions in Coronary Heart Disease Patients Hariyono, Hariyono; Madyawati, Sri Pantja; Romli, Leo Yosdimyati; Tan, Chin Xuan; Fauziyah, Shifa; Sucipto, Teguh Hari
Jurnal Kesehatan Masyarakat Vol. 21 No. 2 (2025)
Publisher : Universitas Negeri Semarang in collaboration with Ikatan Ahli Kesehatan Masyarakat Indonesia (IAKMI Tingkat Pusat) and Jejaring Nasional Pendidikan Kesehatan (JNPK)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v21i2.24553

Abstract

Coronary heart disease (CHD) continues to be a leading cause of hospital readmissions, especially among elderly individuals with low educational attainment and limited understanding of health information. This research aimed to evaluate how cardiac care management influences the frequency of hospital readmission in patients diagnosed with CHD. Employing a quasi-experimental method with a one-group pretest-posttest design, the study involved 100 participants at Jombang Hospital, chosen through purposive sampling. Information regarding readmission rates was gathered before and after the intervention using structured questionnaires and assessed through the Wilcoxon signed-rank test. Before receiving the intervention, 25% of patients had been readmitted more than twice, while none avoided readmission altogether. Following the management program, only 5% experienced multiple readmissions, and 25% reported none. The statistical outcome demonstrated a significant difference (p = 0.002), proving that cardiac care interventions consisting of educational support, family involvement, and consistent follow-up effectively decreased patient readmission. Most participants were aged between 61 and 75, male, and had only completed elementary school, all of which are factors associated with poor disease self-management. This trial confirms that integrated cardiac care management greatly improves the outcomes of recovery and decreases the risk of rehospitalization in CHD patients.