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Journal : Biomedika

UJI PENURUNAN KADAR GLUKOSA DARAH OLEH EKSTRAK ETANOL 70% DAUN BUNCIS (Phaseolus vulgaris L) PADA KELINCI JANTAN YANG DIBEBANI GLUKOSA Kurniawati, Desy; Sutrisna, EM; Wahyuni, Arifah Sri
Biomedika Vol 4, No 1 (2012): Biomedika Februari 2012
Publisher : Universitas Muhamadiyah Surakarta

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Abstract

This research aim to know the descent of blood glucose rate by extract etanol 70% of bean leaf at male rabbit which induced glucose. Research using the method test glucose tolerance of oral was including category research of sham eksperimental with complete random attempt device of unidirectional pattern. This research use 20 male rabbits which divided in 5 treatment group. Group I given by CMC Na 1% as negative control, group II given by acarbose suspensi 2,33 mg/kgBB as positive control, group III, IV and V given by extract etanol 70% bean leaf with each dose 200, 300 and 450 mg/kgBB. Treatment given at once after test animal induced glucose 50% ( 5 ml/1,5 kgBB). Glucose blood rate to define every 30 minute, 0 minute until 300 minutes by using method of enzimatik GOD PAP (Glucose Oxidase Phenol Aminoantipyrina Peroxidase), what was later then analysed with one track Anava and continued by test of LSD (Least Significant Diffeerence) with trust level 95%. Extract etanol 70% bean leaf with dose 200, 300 and 450 mg/kgBB have effect descent of blood glucose rate to negative control of CMC Na 1% (p = 0,000) with each percentage descent of blood glucose rate (% PKGD) 80,88 ± 0,93 ; 82,73 ± 0,67 and 85,32 ± 0,21.Keyword : Extract etanol 70%, bean leaf (Phaseolus vulgaris L), degradation of glucose rate
UJI PRAKLINIK ANTIHIPERURISEMIA SECARA IN VIVO PADA MENCIT PUTIH JANTAN GALUR BALB-C DARI EKSTRAK DAUN SALAM (Syzigium polyanthum Walp) DAN DAUN BELIMBING WULUH (Averrhoa bilimbi L.) Muhtadi, .; Suhendi, Andi; Wahyuningtyas, Nurcahyanti; Sutrisna, EM.
Biomedika Vol 6, No 1 (2014): Biomedika Februari 2014
Publisher : Universitas Muhamadiyah Surakarta

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Abstract

Antihyperuricemia activity in vivo testing has been done of single and combined extracts of Salam (Syzigium polyanthum Walp) and Belimbing Wuluh leaves (Averrhoa bilimbi L.), and also standardized extracts. Salam leaves extraction and Belimbing Wuluh leaves carried by the water solvent. Dried extract of each ingredient had been done antihyperuricemia preclinical in vivo testing against male white mice strain Balb-C induced by potassium oxonate dose of 250 mg / kg b.w. Antihyperuricemia preclinical in vivo testing results showed that the single extracts of Salam and Belimbing Wuluh at a dose of 200 mg / kg b.w. had lower activity levels of uric acid in the blood serum of male white mice, respectively 0.640 and 0.680. While the activity of the combination of extracts of Salam-Belimbing Wuluh is 1.380.Keywords: Antihyperurisemia preclinical in vivo testing; Salam leaves extract, Belimbing Wuluh leaves extract, male white mice Balb-C
EFEK INFUSA AKAR TEMPUYUNG (SONCHUS ARVENSIS) TERHADAP PENURUNAN KADAR ASAM URAT PADA TIKUS PUTIH (RATTUS NORVEGICUS) Retnowati, Kurnia; Sutrisna, EM; Nurmahmudah, Iin Novita
Biomedika Vol 6, No 2 (2014): Biomedika Agustus 2014
Publisher : Universitas Muhamadiyah Surakarta

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Abstract

Tempuyung (Sonchus arvensis) consisted of fiavonoid were effect as obstruct xantine oksidase enzyme, antioxidant captur superoxsidase radical. The fiavonoid total in leave of tempuyung is 0,1044%, in its root have 0,5% fiavonoid and the more is apigenin-7-O-glikosida (3,4,5). This research aim to know effect of infusa root of tempuyung (Sonchus arvensis) to lower the uric acid level at blood serum and infusa root of Tempuyung (Sonchus arvensis) to lower the uric acid level at mouse blood serum compared to allopurinol.This research is laboratory experimental method. The object were 25 Wistar male mice 2-3 months old, 150-200 gr divided into 5 groups. Negative Control group given potassium oxonate by intraperitoneal, Positive Control group given potassium oxonate by intraperitoneal, added by allopurinol 18mg/kgBB, Infusa Concentrated Group 1 given potassium oxonate by intraperitoneal added by infusa root of tempuyung 1,25g/kgBB, Infusa Concentrated Group 2 given potassium oxonate by intraperitoneal added by infusa root of tempuyung 2,5g/kgBB, Infusa Concentrated Group 3 given potassium oxonate by intraperitoneal added by infuse root of tempuyung 5g/kgBB. Executed until one day, where measurement of uric acid of mouse blood serum done before and after treatment. Measurement of uric acid level is done by using spectrophotometer. Obtained to be data to be analysed with Kolgomorov-Smirnov test, One-Way ANOVA and continued with LSD (Least Significant Difference) test with 95% confidence interval (CI). Result of statistical test of research shoe that infusa root of Tempuyung (Sonchus arvensis) dose 1,25g/kgBB, 2,5g/ kgBB, 5g/kgBB have effect to lower the uric acid level at mouse blood serum. Infusa root of Tempuyung (Sonchus arvensis) concentrated 5g/kgBB proportional wih dose allopurinol 18mg/kgBB to lower the uric acid level at mouse blood serum.Keyword: Infusa, root of Tempuyung (Sonchus arvensis), uric acid
UJI EFEK ANTIBAKTERI EKSTRAK ETIL ASETAT DAN KLOROFORM MENIRAN (Phyllanthus niruri Linn) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus ATCC 6538 DAN Escherichia coli ATCC 11229 SECARA in vitro Rahman, Dwiariawan Tauchid; Sutrisna, EM; Candrasari, Anika
Biomedika Vol 4, No 2 (2012): Biomedika Agustus 2012
Publisher : Universitas Muhamadiyah Surakarta

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Meniran is one of traditional plants that contains several compounds such as terpenoid, flavonoid, and alkaloid that role as antibactrial agent. The aims of the research are to determine the antibacteria effect of extract aethyl acetat and chloroform of meniran. This research was conducted as a laboratory experimental by using post test control group design only. The concentration of extract aethyl acetat and chloroform meniran are 5%, 10%, 20%, 40%, and 80%. The result indicate that extract aethyl acetat of meniran have not effect to Escherichia coli. Meanwhile, to Staphyloccus aureus have an effect (p=0,003). The concentration of the extract chloroform meniran are effective to inhibite the growth of Escherichia coli (p=0,029) and Staphylococcus aureus (p=0,004). The extract aethyl acetat of meniran have an antibacteria effect to Staphylococcus aureus but not to Escherichia coli. Whereas, the extract chloroform of meniran have an antibacteria effect to Staphylococcus aureus and Escherichia coli.Keyword : The aethyl acetat and chloroform extract of Meniran (Phyllanthus niruri L.), antibacteria effect, Staphylococcus aureus and Escherichia coli
FARMAKOGENETIK PENGOBATAN BETA 2 AGONIST DAN STEROID PADA PENDERITA ASMA Sutrisna, EM
Biomedika Vol 6, No 2 (2014): Biomedika Agustus 2014
Publisher : Universitas Muhamadiyah Surakarta

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In the worldwide about 300 million people have asthma. β-2 agonists and steroids are widely prescribed drugs in the treatment of asthma. Different responses raises the suspicion of a genetic infiuence on the host’s response to the treatment of this disease. The purpose of this review is to provide a comprehensive understanding of the infiuence of genetic factors on the treatment of asthma. This review is done by the method in Pubmed search using keywords Pharmacogenetic asthma; polymorphism beta agonist AND asthma. The analysis shows that there are several genes that affect treatment response. ADRB2 gene affects the bronchodilatation response for asthmatics treated with β-2 agonist. CRHR1 gene polymorphism, Glucocorticoid-complex genes, and CER2 genes and TBX21 genes affect the response to corticosteroids inhalers.Keywords: Asthma, Pharmacogenetics, Treatment response
UJI EFEK PEMBERIAN EKSTRAK ETANOL 70% BIJI BUAH TERUNG UNGU (SOLANUM MELONGENA L.) TERHADAP KADAR KOLESTEROL HDL SERUM DARAH TIKUS PUTIH JANTAN (RATTUS NORVEGICUS) GALUR WISTAR Fajriyatun, Baiq Nova; Sutrisna, EM; Nirlawati, Dona Dewi
Biomedika Vol 6, No 1 (2014): Biomedika Februari 2014
Publisher : Universitas Muhamadiyah Surakarta

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Abstract

Extract of eggplant fruit (Solanum melongena L.) seed is a plant that has anti-hypercholesterolemia effect on the state of dyslipidemia. One of the compounds contained in them are fl avonoids nasunin which can increase levels of HDL cholesterol (high density lipoprotein). HDL cholesterol is considered as a compound have the ability to remove excess cholesterol from the arteries, and therefore called good cholesterol. This study is a laboratory experimental research using pretest and posttest control group. The sample consisted of 25 rats were divided into 5 groups. Group I is a negative control (-), Group II is a positive control (+), group III was treated 1 (dose 1), group IV was treated 2 (dose 2), and V is the treatment group 3 (dose 3). Each group was given a high-cholesterol diet. Negative and positive control group were not given the extract of fruit seed of eggplant, the treatment groups III, IV, and V were given the extract of fruit seed of eggplant with each dose of 20 mg/kg, 40 mg/kg, 80 mg/kg were given for 4 weeks. HDL cholesterol levels will be measured before and after cholesterol diet and calculated each week after giving of the extract. The data obtained tested with statistical tests One-Way Anova. HDL cholesterol positive control group and the treatment group showed signifi cant differences between groups. Treatment groups 1 and 2 levels of HDL cholesterol lower than the positive control group and the treatment group 3. Extract of eggplant fruit seed dose of 80 mg/kg show an increase in HDL cholesterol levels were signifi cantly (p <0.05). The 70% ethanol extract of eggplant fruit seed at a dose of 80 mg/kg can increase HDL cholesterol levels signifi cantly.Keywords : extract of eggplant fruit seed, hypercholesterol, HDL cholesterol
ANTIOKSIDAN EKSTRAK ETANOL 70% DAUN SYZYGIUM POLYANTHUM (WIGHT) (SALAM) SECARA INVITRO Sutrisna, EM; Trisharyanti, Ika; Munawaroh, Rima; ., Suprapto
Biomedika Vol 7, No 1 (2015): Biomedika Februari 2015
Publisher : Universitas Muhamadiyah Surakarta

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Abstract

Radikal bebas merupakan satu faktor kontributor terjadinya penyakit degenerative. Antioksidan merupakan senyawa yang menghambat efek radikal bebas tersebut. Pada penelitian sebelumnya, ekstrak methanol daun Daun salam mempunyai efek antioksidan. Penelitian ini bertujuan menguji efek antioksidan ekstrak ethanol 70% daun salam (Syzygium polyanthum (Wight)). Metode yang digunakan adalah uji invitro dengan metode DPPH (Diphenyl picrylhydrazyl). Ekstrak dilarutkan dalam ethanol, kemudian dibuat dalam seri konsentrasi 10, 30, 50 dan 70 ug/mL. Masing-masing seri sebanyak 10 ml. Dalam masing-masing larutan tersebut ditambahkan 1 ml larutan DPPH 1 mM. larutan kemudian diinkubasi pada suhu 37°C selama 30 menit. Panjang gelombang yang digunakan adalah 515 nm. Metanol dan DPPH 1mM digunakan sebagai blanko.Untuk pembanding digunakan butylated hydroxytoluene (BHT) konsentrasi 2, 4, 6, 8 ug/mL. Hasil penelitian menunjukkan bahwa ekstrak ethanol 70% daun salam mempunyai efek antioksidan dengan IC 50 27,80 ug/mL.Kata kunci: daun salam (Syzygium polyanthum (Wight) , antioksidan, IC 50
EFEK INFUSA AKAR TEMPUYUNG (SONCHUS ARVENSIS) TERHADAP PENURUNAN KADAR ASAM URAT PADA TIKUS PUTIH (RATTUS NORVEGICUS) Retnowati, Kurnia; Sutrisna, EM; Nurhidayati Mahmuda, Iin Novita
Biomedika Vol 6, No 2 (2014): Biomedika Agustus 2014
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v6i2.274

Abstract

Tempuyung (Sonchus arvensis) consisted of fiavonoid were effect as obstruct xantine oksidase enzyme, antioxidant captur superoxsidase radical. The fiavonoid total in leave of tempuyung is 0,1044%, in its root have 0,5% fiavonoid and the more is apigenin-7-O-glikosida (3,4,5). This research aim to know effect of infusa root of tempuyung (Sonchus arvensis) to lower the uric acid level at blood serum and infusa root of Tempuyung (Sonchus arvensis) to lower the uric acid level at mouse blood serum compared to allopurinol.This research is laboratory experimental method. The object were 25 Wistar male mice 2-3 months old, 150-200 gr divided into 5 groups. Negative Control group given potassium oxonate by intraperitoneal, Positive Control group given potassium oxonate by intraperitoneal, added by allopurinol 18mg/kgBB, Infusa Concentrated Group 1 given potassium oxonate by intraperitoneal added by infusa root of tempuyung 1,25g/kgBB, Infusa Concentrated Group 2 given potassium oxonate by intraperitoneal added by infusa root of tempuyung 2,5g/kgBB, Infusa Concentrated Group 3 given potassium oxonate by intraperitoneal added by infuse root of tempuyung 5g/kgBB. Executed until one day, where measurement of uric acid of mouse blood serum done before and after treatment. Measurement of uric acid level is done by using spectrophotometer. Obtained to be data to be analysed with Kolgomorov-Smirnov test, One-Way ANOVA and continued with LSD (Least Significant Difference) test with 95% confidence interval (CI). Result of statistical test of research shoe that infusa root of Tempuyung (Sonchus arvensis) dose 1,25g/kgBB, 2,5g/ kgBB, 5g/kgBB have effect to lower the uric acid level at mouse blood serum. Infusa root of Tempuyung (Sonchus arvensis) concentrated 5g/kgBB proportional wih dose allopurinol 18mg/kgBB to lower the uric acid level at mouse blood serum.Keyword: Infusa, root of Tempuyung (Sonchus arvensis), uric acid
EFEK EKSTRAK ETANOL 70% DAUN KEMBANG SEPATU (Hibiscus rosa-sinensis L.) DALAM MEMPERPENDEK WAKTU PERDARAHAN DAN WAKTU PEMBEKUAN PADA MENCIT JANTAN GALUR SWISS Aisyah, Riandini; Gunawan, Ryan Budi; Sutrisna, EM
Biomedika Vol 8, No 1 (2016): Biomedika Februari 2016
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v8i1.3023

Abstract

Daun kembang sepatu (Hibiscus rosa-sinensis L.) mengandung senyawa tannin dan flavonoid yang berefek sebagai agen hemostatik/penghentian perdarahan melalui mekanisme vasokonstriksi dan astringent. Penelitian ini dilakukan untuk mengetahui efek ekstrak topikal kembang sepatu terhadap penghentian perdarahan luar pada mencit. Penelitian bersifat eksperimental laboratoris dengan rancangan post test only with control group design. Subyek penelitian adalah daun kembang sepatu (Hibiscus rosa-sinensis L.) dan menggunakan 24 ekor mencit jantan galur Swiss yang dibagi dalam 6 kelompok yaitu : kontrol negatif (aquadest), kontrol positif (Epinefrin), dan kelompok III, IV, V, VI : diberikan ekstrak daun kembang sepatu dengan konsentrasi 5%, 10%, 20% dan 40% diberikan secara topikal. Ekor tikus diberi perlukaan kemudian dicelupkan pada tiap-tiap kelompok untuk diamati dan dihitung waktu perdarahan dan waktu pembekuan. Data yang diperoleh dianalisis dengan uji statistic Oneway Anova dan uji statistic LSD (Least significant Difference). Terdapat perbedaan bermakna dari bleeding timeantara kontrol negatif dengan perlakuan (p.0,000, sementara tidak terdapat perbedaan dalam cotting time  (p,0,313.Kesimpulan: ekstrak etanol 70% daun kembang sepatu (Hibiscus rosa-sinensis L.) konsentrasi 5%, 10%, 20% dan 40% mampu memperpendek waktu perdarahan, namun, tidak berefek memperpendek waktu pembekuan.Kata kunci : ekstrak daun kembang sepatu (Hibiscus rosa-sinensis L.), waktu perdarahan, waktu pembekuan
UJI EFEK EKSTRAK ETANOL 96% ANGGUR MERAH (Vitis vinifera) TERHADAP PENURUNAN KADAR TRIGLISERIDA PADA TIKUS PUTIH (Rattus Novergicus) YANG DIINDUKSI TRITON X-100 Saputra, Frans; Sutrisna, EM; Nurhayani, Nurhayani
Biomedika Vol 8, No 2 (2016): Biomedika Agustus 2016
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v8i2.2914

Abstract

Buah anggur merah diduga memiliki kandungan pterostilbene, resveratrol, proantosianidin dan likopen yang memiliki efek terhadap penurunan kadar trigliserida. Penelitian ini bersifat eksperimental laboratorium dengan metode pre and post test with control group design. Objek penelitian 25 ekor tikus putih jantan, Rattus Novergicus, berat badan 150-200 gram, berumur 3-4 bulan yang dibagi menjadi 5 kelompok dengan teknik simple random sampling, kontrol negatif (aquadest), kontrol positif (Simvastatin 0,2mg/200gramBB/hari), kelompok perlakuan dosis I (100mg/200gramBB/hari), dosis II (250mg/200gramBB/hari), dosis III (500mg/200gramBB/ hari). Ekstrak etanol anggur merah dosis I, dosis II, dosis IIIdapat menurunkan kadar trigliserida darah dengan rerata penurunan secara berturut-turut adalah 147,4mg/dL, 135,2mg/dL, 97,2mg/dL. Pada uji statistic menggunakan one-way ANOVA didapatkan nilai p=0,000 (p<0,05), sehingga terdapat perbedaan signifikan kadar trigliserida darah tikus putih antar kelompok. Ekstrak etanol 96% anggur merah dosis 100mg; 250 dan 500 /200 gramBB/hari dapat menurunkan kadar trigliserida darah tikus putih. Kata kunci :Ekstrak Anggur Merah, Trigliserida, Rattus Novergicus
Co-Authors . Muhtadi . MUHTADI Abd Ghani, Mohd Faiz Abdussalam, Arrizqi Hafidh Amalinda Ayu Faroska Andi Suhendi Andi Suhendi Andi, Andi Suhendi Andriani, Betty Anika Candrasari Annissa, Yanis Nur Arifah Sri Wahyuni Aris Fitriyani Ashshiddiiq, Zaid Ziyaadatulhuda Aulia, Qurrotu A’yuni Awal Prasetyo Ayustine Winarta, Selly Baiq Nova Fajriyatun Baiq Nova Fajriyatun, Baiq Nova Darnoto , Sri Dedi Hanwar DESY KURNIAWATI Desy Kurniawati Devi Usdiana Rosyidah Diana Risanti, Erika Dodik Nursanto Dona Dewi Nirlawati Dwiariawan Tauchid Rahman Dwiariawan Tauchid Rahman, Dwiariawan Tauchid Erna Kristin Esti Dwi Widayanti Faradisa, Nida Fardhiyanti, Fardhiyanti Faroska, Amalinda Ayu Fauziana , Eni Fauziana, Eni Flora Ramona Sigit Prakoeswa Frans Saputra Gunawan, Ryan Budi Hadi, Cahyono Happy Kurnia Permatasari Hasna Zahro Iftikhonsa Ihwan Susila Iin Novita Nurhidayati Mahmuda Ika Trisharyanti Dian Kusumowati Imronudin Imronudin, Imronudin Indra Nuroso Indwiani Astuti Iwan Dwiprahasto Iwan Dwiprahasto Kelik Wardiono Khaeditama Purnama Anwar Khusnul Khotimah Kurnia Retnowati Kurnia Retnowati, Kurnia Listiana Masyita Dewi Mada Sukma Dytho Maimun, Muhammad Halim Maisje Marlyn Kuhu Maryati Maryati Maryati Maryati Masyita Dewi, Listiana Maulindar, Joni Mida Pratiwi Moefida, Noeroel Muchlison Anis Mufidah, Fathin Muhammad Ibrahim Muhtadi Muhtadi Muhtadi MULYOTO PANGESTU Muzakar Isa N Nurhayani Noer Safita Noer Safita Nordin, Noraziah Nur Mahmudah Nurcahyanti . Nurcahyanti Wahyuningtyas Nurcahyanti Wahyuningtyas, Nurcahyanti Nurfauzia, Elita Nadia Nurhaliza, Alfida Aulia Rahma Firdauzy Nurhayani Nurhayani Nurhayani nurhayani Nurhayani, N Nurjanah, Adha Nuroso, Indra Peni Indrayudha Pranesti, Rahma Pratiwi, Betty Intan Purwani Rahmi , Anggraita Nur Rahmi, Anggraita Nur Ratih Pramuningtyas Ratnaningrum, Anna Retno Sintowati - Riandini Aisyah Rima Munawaroh Rini Kuswati Rochmadina Suci Bestari Roslan, Nuruliza Ryan Budi Gunawan Safari Wahyu Jatmiko Safita, Noer Saputra, Frans Selly Ayustine Winarta Setyowatiningsih, Lilik Siti Zulaekah Sri Darnoto Sri Wahyuni Studiviani Dwi Binuko, Raafika Sukma Dytho, Mada Suprapto . Suprapto . Surati Surati Tanti Azizah Sujono Usdiana Rosyidah, Devi Vianti, Melita Febry Wahyu Jatmiko, Safari Wardiono , Kelik Wasifah, Wasifah Werdany, Kusuma Estu Widananda , Clarisa Widananda, Clarisa Widiyanto, Santo Yoseph Didik Yanis Nur Annissa Yuli Kusumawati Yuni Prastyo Kurniati Yuniar, Siska Yusuf Alam Romadhon Zahro Iftikhonsa, Hasna Zaid Ziyaadatulhuda Ashshiddiiq