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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Berkala Ilmiah Pertanian Jurnal Produksi Tanaman Biotropika Journal of Degraded and Mining Lands Management Research Journal of Life Science Jurnal Sumberdaya Alam dan Lingkungan Journal of Tropical Plant Protection AGRIVITA, Journal of Agricultural Science JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Terapan JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) BUANA SAINS Viabel : Jurnal Ilmiah Ilmu-Ilmu Pertanian Agro Bali: Agricultural Journal Nusantara Science and Technology Proceedings Jurnal HPT (Hama Penyakit Tumbuhan) International Journal of Agriculture System

Luqman Qurata Aini

Departemen Hama Dan Penyakit Tumbuhan Fakultas Pertanian Universitas Brawijaya

Author-ID : 5363792

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Engineering Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics

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KARAKTERISASI BAKTERI PENYEBAB PENYAKIT HAWAR DAUN EDAMAME DI JEMBER Rachmi Masnilah; Abd Latif Abadi; Tutung Hadi Astono; Luqman Qurata Aini
Berkala Ilmiah Pertanian Vol 1 No 1 (2013): AGUSTUS
Publisher : Jember University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (218.477 KB)

[ENGLISH] One among important diseases in Edamame soybean is bacterial bligh. The disease is relatively new and causes significant loss of soybean yield. This research was aim to know the characterize the pathogen of bacterial blight in Jember. Survey was one in several soybean field in Jember and the plant sample was culture on King's B medium to grow the pathogen. Identification was done through pysiological and biochemistry assay. The result showed that the pathogen of bacterial blight disease on Edamame soybean di Jember (Panti, Sukorambi, Wirolegi, Ajung, and Sumbersari) was Pseudomonas syringae pv. glycinea with properties as Gram negative, produce fluorescent pigment on King's B medium, grew on 20-40O C, pH range of 4.5-8.5, tolerant on 0.5-2% NaCl and also pathogenic and virulent on Edamame while reinoculated to Edamame. Keywords: Edamame, bacterial blight, soybean [INDONESIAN] Salah satu penyakit penting pada kedelai khususnya edamame ialah hawar daun bakteri, penyakit ini merupakan penyakit yang relatif baru dan menyebabkan kerugian yang cukup signifikan. Penelitian ini bertujuan untuk mengetahui karakteristik bakteri penyebab penyakit hawar daun edamame di daerah Jember. Penelitian diawali dengan pengamatan gejala di lapangan di beberapa lokasi di daerah Jember. Bagian tanaman yang sakit dibawa ke laboratorium, selanjutnya dilakukan isolasi pada medium King B. Identifikasi dilakukan dengan serangkaian pengujian fisiologi dan biokimia. Berdasarkan pengujian yang telah dilakukan disimpulkan bahwa bakteri penyebab penyakit hawar daun edamame di Jember (Panti, Sukorambi, Wirolegi, Ajung, dan Sumbersari) ialah Pseudomonas syringae pv. glycinea yang bersifat gram negatif, mampu membentuk pigmen fluoresen pada medium king B, mampu tumbuh baik pada kisaran suhu 20-400 C, mempunyai kisaran pH 4,5-8,5, toleran pada kandungan NaCl 0,5-2%, serta bersifat patogenik dan virulen pada tanaman edamame. Keywords: Edamame; Hawar Daun; Kedelai How to citate: Masnilah R, AL Abadi, TH Astono, LQ Aini. 2013. Karakterisasi bakteri penyebab penyakit hawar daun edamame di Jember. Berkala Ilmiah Pertanian 1(1): 10-14.

Efektifitas Pestisida Biologis Bacillus Cereus dan Bacillus Megaterium. sebagai Pengendali Spodoptera litura Fabr (Lepidoptera: Noctuidae) I Made Indra Agastya; Aminudin Afandhi; Luqman Qurata Aini
Jurnal Penelitian Pertanian Terapan Vol 17 No 2 (2017)
Publisher : Politeknik Negeri Lampung.

This research was conducted in vitro in the laboratory. Studies conducted in the Laboratory of Bacteriology Department of Plant Pests and Diseases of the Faculty of Agriculture, University of Brawijaya, on the effectiveness of the bacteria Bacillus cereus and Bacillus megaterium as biological pesticides controlling Spodoptera litura. The objective of this study was to determine the effectiveness of the bacteria Bacillus sp as biological control. Effectiveness pesticide was measured by testing the incubation period and mortality in larvae of S. litura instar 3. This study used a completely randomized design (CRD), conducted observations every 6 hours until the larvae dead. The results showed that the percentage of mortality of S. litura reached 94.66% compared to the control, while the incubation time of the bacteria B. cereus cause disease until 29.84 hours. B. cereus and B. megaterium have the ability to incubate third instar larvae of S. litura up to 29.84 hours and caused the death of larvae up to 94.66%. Keywords: Biological pesticides, Bacillus cereus, Bacillus megaterium, entomopatogen, Spodoptera litura

Application of bacterial isolates to mitigate the effects of salt stress on red chilli growth and yields Nurul Aini; Wiwin Sumiya Dwi Yamika; Luqman Qurata Aini; Puput Wahyuningsih
Journal of Degraded and Mining Lands Management Vol 6, No 3 (2019)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2019.063.1771

The objective of this research was to study the effect of bacterial isolates on red pepper at the saline condition. The research conducted in the station of Brawijaya University in Jatikerto village, Malang regency. The study used a randomized block design with bacteria isolated from the soil of saline-prone regions of Lamongan, in coastal East Java Indonesia. The treatments consisted of P0: saline soil without bacteria, P1: saline soil + bacteria SN 13, P2: saline + bacteria SN 22, P3: saline soil + bacteria SN 23, P4: saline soil + bacteria (SN 13 + SN 22), P5: saline soil + bacteria (SN 13 + SN 23), P6: saline soil + bacteria (SN 22 + SN 23), and P7: saline soil + bacterial (SN 13 + SN 22 + SN 23). Red chilli variety used was Gada MK F1. The results showed that the application of bacterial isolates increased leaf area, dry weight of roots and shoot. Uptake of N, P, K and Na also increased with bacterial isolate application. The number of fruit and fruit weight increased by 11.94% and 15.93%, respectively, compared with no bacteria. Proline content increased with the application of bacterial, while the capsaicin content decreased.

Eating Behavior of Imago Aulacophora similis Oliver on Cucumber (Cucumis sativus L.) with Treatment Plant Growth Promoting Rhizobacteria (PGPR) Dwi Wulandari; Luqman Qurata Aini; Hagus Tarno
Research Journal of Life Science Vol 7, No 3 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.03.10

The cucumber harvests failure occurred mainly due to the destruction of crops by the leaf-eating beetle A. similis Oliver. Many ways are taken especially pesticides that are synthesized widely because it is considered the fastest and most potent to overcome pest disorders. Based on, one of the control techniques that can be used is PGPR, which is a group of bacteria that can be utilized to strengthen plants against pest attacks and plant diseases, so research needs to be done by using this technique to change the feeding behavior of the A. similis Oliver beetle on cucumber plants. The research was conducted at the Green House Department of Pest and Plant Diseases University of Brawijaya Malang in September 2018 to March 2019. It was conducted using Randomized Block Design (RBD) 5 treatment, i.e., PGPR, urea fertilizer and control (without treatment). Based on the results of the study shows that: Application of PGPR on cucumber plant can reduce damage on either leaves or flower due to the eating behavior of imago A. similis Oliver and Application of PGPR can increase the cucumber plant growth during the eating behavior of imago A. similis Oliver.

The Potency of UB Forest Bacteria as Chitinolytic Bacteria to Inhibit Anthracnose Disease on Cayenne Pepper Nava Karina; Achmad Roekhan; Cindy Diah Ayu Fitriana; Luqman Qurata Aini
Research Journal of Life Science Vol 7, No 1 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.01.5

Anthracnose disease is a major disease in cayenne pepper triggered by fungal pathogen Colletotrichum capsici which can cause yield losses of up to 90%. Control of disease generally utilizes synthetic fungicides, but it damages the environment and human health. Hence, alternative controls that are ecofriendly are required. One of the attempts is to make use of chitinolytic bacteria's potential. This study aimed to screen UB Forest chitinolytic bacteria and to determine their potential in suppressing anthracnose in cayenne pepper. Stages of research include a screening of 78 UB Forest bacteria as chitinolytic bacteria, selection of chitinolytic bacteria that are antagonistic against C. capsici, inhibition test of chitinase crude extracts against C. capsici growth, controlling anthracnose disease in cayenne using chitinolytic bacteria, and molecular identification. Based on the results of the selection, there were 76 isolates of UB Forest bacteria classified as chitinolytic bacteria, 74 bacteria antagonistic against C. capsici. Chitinase crude extracts significantly affected C. capsici growth inhibition compared to fungicide treatment. The application of Pseudomonas aeruginosa (UB 11) and Bacillus cereus (UB 12) can control anthracnose disease in cayenne pepper with the intensity of 41.67% and 38.89% respectively with the efficacy level of 58.33% and 61.11%.

Exploration and Antifungal Assay of Endophytic Fungi as Biocontrol of Onion Purple Blotch Disease Caused by Alternaria porri (Ell) Cif In Vitro Wita Firdausi; Liliek Sulistyowati; Luqman Qurata Aini
AGRIVITA, Journal of Agricultural Science Vol 43, No 1 (2021)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v43i1.2838

Purple blotch disease caused by Alternaria porri is the main destructive foliar disease of genus Allium, causing significant losses in yield of the crops. Recently, purple blotch disease is controlled by synthetic fungicides. However, fungicides have negative effects on the environment. Endophytic fungi can be used as an alternative control because a close symbiosis with the internal tissue of the host can minimize competition in new and complex ecosystems. This study aimed to explore and identify endophytic fungi that have the highest inhibition ability against A. porri and investigate the antagonistic mechanism. The method used in this study is an exploration of endophytic fungi, isolation of A. porri, in vitro antagonism tests, observation of the antagonistic mechanism, extraction of crude protein, SDS-PAGE, and identification. The antagonistic fungi that had the highest inhibition ability were identified as Penicillium citrinum with an inhibitory of 60.04%. Crude protein extracted from P. citrinum which showed inhibitory activity against A. porri is saturation level of ammonium sulfate 80% with a molecular weight of 40 kDa. This study implies that P. citrinum can inhibit the growth of A. porri through its anti fungi compounds. Further in vivo assays or field trials will need to be conducted in future studies.

Mycoparasitic Activity of Indigenous Trichoderma virens Strains Against Mungbean Soil Borne Pathogen Rhizoctonia solani: Hyperparasite and Hydrolytic Enzyme Production Alfi Inayati; Liliek Sulistyowati; Luqman Qurata Aini; Eriyanto Yusnawan
AGRIVITA, Journal of Agricultural Science Vol 42, No 2 (2020)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v0i0.2514

Rhizoctonia solani is one of the harmful pathogens on mungbean, which is very challenging to be controlled. T. virens has been studied intensively and has great potency to control R. solani through mycoparasitism. Seven strains of T. virens isolated from various rhizospheres were tested for their mycoparasitic potential by observing their hyperparasitism and the production of hydrolytic enzymes. All strains showed the ability to suppress the growth of R. solani on dual culture assay as well as on culture filtrate test with the inhibition ability from 43.8 to 68.6% on the dual culture assay and from 22.2 to 71.1% on the culture filtrate assay. Inter-fungal interaction, which was observed by an electron microscope, showed hyperparasitic action of T. virens against R. solani involved the formation of the knob-like structure followed by the growth of Trichoderma hyphae inside host mycelia, coiling, lysed cell wall, and swollen of mycelial tips. Mycoparasitism of T. virens also correlated with the synthesis of hydrolytic enzymes, such as cellulase and chitinase, which influenced the overall hyperparasitic ability of T. virens against the pathogen. Based on in vitro assay, the Tv3 strain proposed as a promising strain to control R. solani due to its high growth inhibition and relatively high cellulase and chitinase productionse.

KEMAMPUAN Bacillus subtilis dan Pseudomonas fluorescens DALAM MELARUTKAN FOSFAT DAN MEMPRODUKSI HORMON IAA (Indole Acetic Acid) UNTUK MENINGKATKAN PERTUMBUHAN TANAMAN TOMAT Istiqomah Istiqomah; Luqman Qurata Aini; Abdul Latief Abadi
BUANA SAINS Vol 17, No 1 (2017)
Publisher : Universitas Tribhuwana Tunggadewi

Tomato is an important horticultural commodity in Indonesia and plays strategic role in fulfilling daily needs of the public. The increase in production of tomato can be done by optimizing the provision of nutrients that support growth. The ability of bacteria as biological fertilizer to dissolve phosphate and produce IAA (Indole Acetic Acid) are important criteria for the use of bacteria as agents of plant growth promoters. The purpose of this study was to determine the ability of some isolates of Bacillus subtilis and Pseudomonas fluorescens in dissolving phosphate and produce IAA to enhance the growth of tomato. Phosphate test results showed that all isolates of B. subtilis and P. fluorescens capable of dissolving phosphate with the highest value indicated by P. fluorescens UB-PF5 (18 mm). All isolates of bacteria capable to produce IAA. The resulting value IAA ranged from 0.69 to 1.09 ppm with the highest value produced by B. subtilis UB-ABS2 (1.09 ppm). The test results on the parameters of root weight, root length, plant height and number of leaves showed that tomato treated with B. subtilis and P. fluorescens increased significant growth compared with watering only with distilled water. The highest increased root weight and root length were resulted by P. fluorescens UB-PF5 with values 97.10% and 64.83%, respectively. The highest increased plant height and number of leaves length were resulted by P. fluorescens UB-PF6 with values 38,80% and 30,7%, respectively.

KEMAMPUAN DUA SPESIES Enterobacter sp. SEBAGAI BAKTERI PEMACU PERTUMBUHAN TANAMAN KENTANG (Solanum tuberosum L.) PADA KONDISI CEKAMAN KEKERINGAN Khoirun Enisa Maharina; Nurul Aini; Luqman Qurata Aini
BUANA SAINS Vol 17, No 1 (2017)
Publisher : Universitas Tribhuwana Tunggadewi

Enterobacter xiangfangensis and Enterobacter cloaceae are known to have the ability as a phosfat solubilizing and producing hormone IAA. Their also have ability to survive under drought stress in vitro assay conditions. Both of these bacteria has good potential as a potato plant growth promoting bacteria, especially in drought stress conditions. This is an alternative to improve tolerance and growth of the potato crop in drought stress conditions. The results showed that the level of drought stress 75% of field capacity can boost the potential of endophytic bacteria E. xiangfangensis in increasing leaf area, plant dry weight, root length and physiological activity i.e total phosphorus content in potato plants. Endophytic bacteria E. xiangfangensis is better to increase potato plant height than endophytic bacteria E. cloaceae. These results are supported by the results of potential test of E. xiangfangensis which have the advantage of producing hormones IAA compared with E. cloaceae. Furthermore, in drought stress conditions 50% of field capacity and 75% of field capacity have different effects on the observations of root length growth parameters potato plants.

The Synergy Between Several Bacteria Isolated from the Biodiversity of UB Forest Coffee Litter in Vitro Gallyndra Fatkhu Dinata; Luqman Qurata Aini; Abdul Latief Abadi
Nusantara Science and Technology Proceedings Bioinformatics and Biodiversity Conferences (BBC)
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.0704

Exploration of bacteria from coffee litter in UB Forest has several new studies, which have the ability as a biocontrol agent. From previous research, it was known the potential of coffee litter bacteria as the single biological control against basal rot in shallots. This study aims to evaluate the ability of bacteria to synergize with other beneficial bacteria of coffee litter. The research consisted of the preparation of coffee litter bacteria isolates and synergy tests using the cross streak method. Based on the research results, not all bacteria can form a consortium. Bacteria that can synergize with each other are Pseudomonas sp., and Alcaligenes faecalis. Three bacteria that cannot be synergized with each other, are Bacillus mycoides, Clostridium sp. and Erwinia sp which produces clear zones up to more than 30 mm. This is preliminary research before starting a bacterial consortium. With this research, it is expected to be a solution for biological control from a consortium of beneficial bacteria from exploration in UB Forest.

Co-Authors Abd Latif Abadi Abd Latif Abadi Abdul Cholil Abdul Cholil Abdul Karim Fanani Abdul Latief Abadi Abdul Latief Abadi Abdul Latief Abadi Achmad Noerkhaerin Putra Achmad Roekhan Adi Setiawan Agung Nugroho Agung Wahyu Susilo Aini Nurul Aini, Febrilia Nur Akhmad Rizali Albert Fernando Sitorus Alda Dwi Aprilia Alfi Inayati Aminudin Afandhi Aminudin Afandhi Anastasya, Nabilla Alya Andhy Handoko Andhy Handoko Anton Muhibuddin Apriyani, Aliffia Nuraita Ardiyan Taruna Arifah, Fitri Ariffin Noor Sugiharto Arifin Noor Sugiharto Aris Budiman Asnawi Asnawi Asparita, Yulia Dwi Auliya Zendhabad Aulya Retno Setyari Aulya Retno Setyari Bayu Setyawan, Bayu Berliana, Sonia Bogi Diyansah Bosha, Mohammed Cindy Diah Ayu Fitriana Cokorda Javandira Cokorda Javandira Daniel Happy Putra Dayanti, Ayu Ike Dewi, Fibrianti Shinta Dian Eka Kusumawati Dianti, Sylvia Dianti, Sylvia Herli Dinata, Gallyndra Fatkhu Diyana Lestari Dwi Wulandari Dyah Ayu Kusuma Wardani Eka Kartini Eriyanto Yusnawan Eriyanto Yusnawan Fibrianti Shinta Dewi Firdausi, Wita Galuh Setyanto Pradana Galuh Setyanto Pradana Ganestya Indina Sari Ganestya Indina Sari Hagus Tarno Hapsari, Femita Hasanah, Ifa Maulidah Husna Fikriya Baroroh I Made Indra Agastya Ichsan Firmansyah Imam Chanif Imam Chanif, Imam Indah Anita Sari Irisa Trianti Irisa Trianti Ismat Ishak Istiqomah Istiqomah Istiqomah, Istiqomah Ito Fernando Ito, Satoshi Iva Dewi Lestariningsih, Iva Dewi Izza, Junda Fauzul Karuniawan Puji Wicaksono Kestrilia Rega Prilianti Khoirun Enisa Maharina Khoirun Enisa Maharina Khoirunisa, Fenti Rahma Kusuma, Restu Rizkyta Liliek Sulistyowati Lilis Suryani Ma'alifah, Nur Maharina, Khoirun Enisa Minal Maimanah Mintarto Martosudiro Mochammad Syamsul Hadi Mochammad Syamsul Hadi Muhammad Akhid Syibâ€™li Muhammad Akhid Syibli Muhammad Febriansyah Muhidin, Ahmad Nava Karina Nindi Kurniasari Novia Dwirani Novie Utami Asputri Nugroho Sulistyo Putro Nugroho Sulistyo Putro Nur Azizah Nurul Aini Nurul Aini Nurul Aini Nurul Aini Oktaviani, Rahmania Pamungkas, Bayu Aji Paramyta Nila Permanasari, Paramyta Nila Prabowo, Matheus Randy Prillianti, Kestrillia Rega Puput Wahyuningsih Rachmi Masnilah Rahmadani, Pradina Rahman, Fakih Nur Ramadhan, Varotama Putra Ramadhani, Fatchuliani Safitri Rania, Khansa Diva Ratri Kusuma Devi Ratri Kusuma Devi Rida Iswati Rifani Rusiana Dewi Rina Rachmawati Rina Rachmawati Roekhan, Achmad Rudin, Sofyan Sudirman Saitama, Akbar Sallygresya Theodora Dwifelita Matondang Santoso, Verisca Agil Winanda Sektiono, Antok Wahyu Serdani, Army Dita Setyono Yudo Tyasmoro Sheila Desi Kharisma Sheila Desi Kharisma Silfiani, Intan Siska Irhamnawati Pulogu Soemarno Soemarno Suharjono Sukmarani, Elok Syamsuddin Djauhari Syamsul Arifin Syarifah Maulidya Rahmah Syib'li, Muhammad Akhid Syibli, Muhammad Akhi Syib`li, Muhammad Akhid Syib’li, Muhammad Akhid Taruna, Ardiyan Tatiek Wardiyati Theresia Rani Kartika Ayu Tita Widjayanti Toto Himawan Toto Himawan Tri Ardiyati Tri Ardiyati Tri Ardyati Trisnawati, Luh Putu Adi Tutung Hadi Astono Tutung Hadiastono Tutung Hadiastono Udayana, Cicik Uma Khumairoh Wardiyati, Tatiek Wita Firdausi Wiwik Jatnika Wiwin Sumiya Dwi Yamika Wiwin Sumiya Dwi Yamika Wiwin Sumiya Dwi Yamika Wiwit Wicaksono Jati Yani Kurniawan, Yani Yogo Setiawan Zainudin Zainudin Zainudin Zainudin Zainul Abidin

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