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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Agroland : Jurnal Ilmu-Ilmu Pertanian Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Journal of Indonesian Tourism and Development Studies Jurnal Produksi Tanaman JTSL (Jurnal Tanah dan Sumberdaya Lahan) Research Journal of Life Science Jurnal Pembangunan dan Alam Lestari Plantropica: Journal of Agricultural Science Journal of Tropical Plant Protection AGRIVITA, Journal of Agricultural Science JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Journal of Information Technology and Computer Science BUANA SAINS Viabel : Jurnal Ilmiah Ilmu-Ilmu Pertanian Agro Bali: Agricultural Journal Jurnal HPT (Hama Penyakit Tumbuhan)
Abdul Latief Abadi
Departemen Hama Dan Penyakit Tumbuhan Fakultas Pertanian Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Electrical & Electronics Engineering Engineering Environmental Science Mathematics Medicine & Pharmacology Physics Social Sciences

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Effectiveness of Binahong (Anredera cordifolia) Leaf Extract in Suppressing The Growth of Xanthomonas oryzae and The Occurrence of Bacterial Leaf Blight Disease in Rice Plant (Oryza sativa) Khotimah, Dwi Kusnul; Abadi, Abdul Latief; Choliq, Fery Abdul
Journal of Tropical Plant Protection Vol. 4 No. 1 (2023)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2023.004.1.4

Abstract

Rice, as a staple food, is widely produced across Indonesia. However, bacterial leaf blight (BLB) in rice is caused by Xanthomonas oryzae pv. oryzae (Xoo) has been reported to cause significant crop yield losses. The continuous use of chemical bactericides to control BLB is not environmentally sustainable. This study investigated the potential of the Binahong plant as a botanical pesticide to suppress the growth of Xoo bacteria. Binahong is readily available in Indonesia and can be propagated generatively and vegetatively. The research employed a Completely Randomized Design (CRD) experimental method to evaluate the effectiveness of Binahong leaf extract against Xoo pathogens in vitro at various concentrations (0.5%, 1%, 1.5%, 2%) alongside positive and negative controls. In-vivo observations were made on several parameters: seed germination percentage, disease incidence, incubation period, and plant length. Data analysis revealed that Binahong leaf extract inhibited the growth of Xoo bacterial colonies in vitro at all tested concentrations (0.5% to 2%). Furthermore, soaking rice seeds in Binahong leaf extract was effective in vivo at all concentrations, demonstrating significant differences compared to the negative control and no significant differences compared to the positive control.
Potential of Siam Weed Leaves (Chromolaena odorata) in Suppressing Anthracnose Disease (Colletotrichum gloeosporioides) in Shallot (Allium ascalonicum L.) in In Vitro Mayasari, Paulina Akita; Abadi, Abdul Latief; Choliq, Fery Abdul
Journal of Tropical Plant Protection Vol. 4 No. 2 (2023)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2023.004.2.2

Abstract

Anthracnose disease (Colletotrichum gloeosporioides) is one of the important diseases in shallots. Generally, farmers use synthetic fungicides. Thus, Siam weed (Chromolaena odorata) leaf extract can be used to reduce it because it has antifungal compounds. This study aimed to examine the effect of siam weed leaf extract on the growth of C. gloeosporioides and its antifungal compounds. This research used a completely randomized design (CRD). It would be continued with the SRD test at the 5% level if the result was significantly different. Treatment consisted of 0, 2, 4, 6, 8, and 10% Siam weed leaf extract with four replications. Bioactive compounds in siam weed leaves were tested by the UPT Herbal Materia Medica Batu Laboratory and Chem-Mix Pratama Laboratory, Yogyakarta. Probit analysis was also used to obtain the LC50 value. The results showed that 10% concentration was the most effective treatment because it produced a fungicidal effect on C. gloeosporioides. However, a 2.09% concentration of siam weed leaf extract was able to cause 50% of C. gloeosporioides' mortality. In addition, siam weed leaf extract contains antifungal compounds, such as tannins, phenols, flavonoids, alkaloids, and saponins.
IDENTIFICATION OF BACTERIAL WILT AND LEAF BLIGHT DISEASE ON MAIZE (Zea mays) FOUND IN KEDIRI, INDONESIA Aini, Luqman Qurata; Suryani, Lilis; Sugiharto, Arifin Noor; Abadi, Abdul Latief
AGRIVITA Journal of Agricultural Science Vol 35, No 1 (2013)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v35i1.245

Abstract

Recently, a new bacterial disease of maize (Zea mays) was observed in Kediri, East Java, Indonesia. Infected plants showed wilt symptoms occasionally accompanied by leaf blight. This study aims to characterize the causal agent of bacterial wilt and leaf blight of maize observed in Kediri. Gram-negative, facultative anaerobic bacteria were isolated from the diseased tissues. All bacterial strains (KD1A, KB2A, KD1, KD4, KB1) gave positive result both in the hypersensitive response and pathogen-nicity assays. However, only KD1 and KB1 strains could be re-isolated from the diseased tissues. Based on several physiological and biochemical assays, the bacteria resembled Pantoea agglomerans. Moreover, the strains showed negative result on PCR amplification using HRP1d and HRP3r, a primer pair specific for detection of P. stewartii subsp. stewartii. Analysis of 16S rRNA gene sequence of KD1 and KB1 showed highest homology at 88% and 90%, respectively to P. agglomerans strain DSM 3493 (NR 0419781).  The homology values were too low to conclude that the bacteria were similar to P. agglomerans. These results suggest that bacterial pathogens isolated from maize in Kediri were strains of Pantoea sp. Keywords: maize, Pantoea sp., wilt, leaf blight
THE POTENCY OF Bacillus sp. AND Pseudomonas sp. AS BIOLOGICALCONTROL AGENTS AGAINST CORN LEAF BLIGHT DISEASE CAUSED BY Pantoea sp. Javandira, Cokorda; Aini, Luqman Qurata; Sugiharto, Ariffin Noor; Abadi, Abdul Latief
AGRIVITA Journal of Agricultural Science Vol 35, No 2 (2013)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v35i2.291

Abstract

One of new biotic constraints in corn production in Indonesia is leaf blight disease caused by Pantoea sp. which is needed to be controlled. The purpose of this research is to study the potential of Bacillus sp. and Pseudomonas sp. as biological control agents against corn leaf blight caused by Pantoea sp. The results showed that all bacterial strains of Bacillus sp. and Pseudomonas sp. have potential in inhibiting the growth of Pantoea sp. by showing the clear zone on the agar plate. The antibiosis types are bactercide or bacteriostatic. On pot experiment all bacterial strains showed the reduction of the disease incidence at the same level compared with that of bactericide streptomycin suphate. All bacterial strains as well as bactericide could reduce the disease incidence at 18-24% compared with that of control (aquades treatment only). The results suggest that all bacterial strains are potential as biological control agent against leaf blight disease on corn leaf caused by Pantoea sp.Keywords: Biological control, Bacillus sp., Pseudomonas sp. and Pantoea sp.
Efficacy of Biofungicide with Active Ingredients Trichoderma sp. Against Late Blight Disease (Phytophthora infestans) in Potato Plants Abadi, Abdul Latief; Trianti, Irisa; Choliq, Fery Abdul; Sektiono, Antok Wahyu; Yulianti, Novi Dwi
Agro Bali : Agricultural Journal Vol 7, No 1 (2024)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v7i1.1591

Abstract

The cultivation of potatoes in Indonesia faces challenges, particularly a decline in production attributed to the devastating impact of late blight caused by the fungus P. infestans. Traditional control methods involve the frequent use of chemical pesticides, which pose environmental and health risks. To address this, the research aimed to assess the efficacy of biofungicide containing Trichoderma sp. as a biological agent in controlling late blight on potatoes. The study, conducted in Sumberbrantas Village, Bumiaji District, Batu City, East Java, and the plant disease laboratory at Brawijaya University from January to April 2023, employed both in vivo and in vitro experiments. A Randomized Block Design (RBD) was used for in vivo research, while a Completely Randomized Design (CRD) was employed for in vitro studies, each comprising five concentration treatments with five repetitions. Parameters such as disease intensity, efficacy level, potato production, and biofungicide inhibition were observed. Analysis of variance and the Tukey Honestly Significant Difference (HSD) test were applied to the data and processed using Microsoft Excel. The results indicated that biofungicide with Trichoderma sp. concentrations of 6 ml/l, 4.5 ml/l, 3 ml/l, and 1.5 ml/l effectively reduced the intensity of late blight on potatoes, with efficacy levels exceeding 50%. The highest potato production occurred with a concentration of 6 ml/l, yielding 15.44 tons/ha, followed by 14.67 tons/ha for the 4.5 ml/l concentration. The biofungicide with a concentration of 6 ml/l exhibited the lowest disease intensity at 14.28% and the highest efficacy at 83.08%. Inhibition tests demonstrated that concentrations of 6 ml/l and 4.5 ml were highly effective, inhibiting P. infestans growth by an average of 62.04% and 59.90%, respectively. These findings highlight the potential of biofungicide with Trichoderma sp. in managing late blight on potatoes, providing a sustainable and environmentally friendly alternative to chemical pesticides.
TEKNIK APLIKASI Trichoderma Sp. TERHADAP PERTUMBUHAN DAN HASIL TANAMAN KENTANG (Solanum tuberosum L.) Baihaqi, Achmad; Nawawi, Mochammad; Abadi, Abdul Latief
Produksi Tanaman Vol. 1 No. 3 (2013)
Publisher : Jurusan Budidaya Pertanian Fakultas Pertanian Universitas Brawijaya

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Abstract

Tujuan dari Penelitian ini ialah untuk mengetahui interaksi konsentrasi dan waktu aplikasi Trichoderma sp. terhadap pertumbuhan dan hasil tanaman kentang (Solanum tuberosum L.). Penelitian ini dilaksanakan di Kebun Percobaan Cangar, Fakultas Pertanian Universitas Brawijaya berada di Desa Sumberbrantas, Kecamatan Bumiaji, Kota Batu pada ketinggian tempat 1650 mdpl. Penelitian ini dilaksanakan pada bulan Mei sampai Agustus 2012. Penelitian ini menggunakan Rancangan Petak Terbagi (RPT) Faktorial yakni dengan dua faktor. Faktor pertama sebagai petak utama ialah konsentrasi Trichoderma sp. cair terdiri atas T1: konsentrasi 5 ml.l-1, T2: konsentrasi 10 ml.l-1. Faktor kedua sebagai anak petak ialah waktu aplikasi Trichoderma sp. cair terdiri atas W0: aplikasi sekali, W1: aplikasi 2 hari sekali, W2: aplikasi 4 hari sekali, W3: aplikasi 6 hari sekali, W4: aplikasi 8 hari sekali. Terdapat 10 perlakuan kombinasi dengan setiap perlakuan diulang sebanyak tiga kali dan diperoleh 30 petak percobaan. Berdasarkan hasil percobaan terdapat interaksi nyata antara konsentrasi Trichoderma sp. dan waktu aplikasi terhadap saat muncul serangan penyakit hawar daun, namun tidak berpengaruh nyata terhadap pertumbuhan dan hasil umbi kentang. Hasil bobot umbi kentang tertinggi pada konsentrasi Trichoderma sp. 10 ml l-1 dengan waktu aplikasi delapan hari sekali (T2W4) ialah 2,34 ton ha-1.
UJI KETAHANAN 7 KLON TANAMAN KENTANG (Solanum Tuberosum L.) TERHADAP PENYAKIT HAWAR DAUN (Phytopthora Infestans (Mont.) de Barry) Vini Nathasia, Anak Agung; Abadi, Abdul Latief; Wardiyati, Tatiek
Produksi Tanaman Vol. 1 No. 6 (2013)
Publisher : Jurusan Budidaya Pertanian Fakultas Pertanian Universitas Brawijaya

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Abstract

Ketahanan tanaman terhadap penyakit merupakan salah satu sifat unggul dari suatu varietas tanaman yang diwariskan yang dapat dilihat dari berkurangnya kejadian dan atau keparahan penyakit. Tujuan penelitian ialah untuk mengetahui dan mendapatkan ketahanan 7 klon tanaman kentang (Solanum tuberosum L.) terhadap penyakit hawar daun. Hipotesis yang diajukan yaitu terdapat klon tanaman kentang (Solanum tuberosum L.) yang toleran terhadap penyakit hawar daun. Penelitian dilaksanakan pada bulan Agustus 2012 - Januari 2013 di Desa Kalitejo, Kecamatan Tosari, Pasuruan, menggunakan Rancangan Acak Kelompok (RAK) dengan 7 perlakuan dan 4 kali ulangan. Klon yang di uji UB1, UB2 (Granola transgenik), UB3 (Atlantik transgenik),  UB4 (Superjhon transgenik), Superjhon, Atlantik, dan Granola. Hasil penelitian menunjukkan klon UB1 dan UB4 (Superjhon transgenik) merupakan klon yang toleran terhadap serangan hawar daun, dengan produksi berdasarkan hasil umbi per hektar UB1 (36,08 ton/ha), UB4 (Superjhon transgenik) (20,67 ton/ha).   Kata kunci: Kentang, Klon, Uji ketahanan, Phytopthora infestans
Potensi Trichoderma sp. Indigenus Gorontalo sebagai Dekomposer Limbah Tanaman Jagung Iswati, Rida; Abadi, Abdul Latief; Aini, Luqman Qurata; Soemarno, Soemarno; Asnawi, Asnawi; Pulogu, Siska Irhamnawati; Rudin, Sofyan Sudirman
Jurnal Ilmu Pertanian Indonesia Vol. 29 No. 2 (2024): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.29.2.163

Abstract

Using decomposers of fungi to increase the composting process is very necessary. The research aims to determine the potential of Trichoderma sp. Gorontalo local isolate as a decomposer of maize stover. The research was carried out from November 2022‒January 2023 at the Biological Agents Laboratory, Agricultural Plant Protection Center, Gorontalo Province Agricultural Department. This research determined the ability of six isolates of Trichoderma, namely TZ11DI1 (T. asperellum), TZ21BN2 (T. breviconvenctum), TZ21BT1 (T. virens), TZ12PO1 (T. ghanence), TZ21DU1 (T. reesei), TZ21LU1 (T. dorothopsis), and control (EM4) to produce cellulase and as a maize stover decomposer. The parameters observed were the clear zone in the isolate growing media as well as the physical and chemical characteristics of the compost produced. The results showed that Trichoderma isolates produced cellulase and potential as a decomposer for maize stover, as indicated by the ability to increase NPK nutrient levels and reduce the C/N ratio of maize stover compost. Keywords: compost, decomposer, maize stover, Trichoderma
Peran Trichoderma spp. sebagai Agen Biokontrol pada Penyakit Rebah Semai dan Biostimulan Kedelai: The Role of Trichoderma spp. as a Biocontrol Agent of Damping Off Disease and Soybean Biostimulant Maknunin, Lu'lu'il; Abdul Latief Abadi; Fery Abdul Choliq
Jurnal Fitopatologi Indonesia Vol. 20 No. 6 (2024): November 2024
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.20.6.304-314

Abstract

The Role of Trichoderma spp. as a Biocontrol Agent of Damping Off Disease and Soybean Biostimulant   Domestic demand for soybeans in Indonesia continues to increase each year, yet the average production has declined. To meet national needs, the government must even import soybeans. One of the causes of low soybean production is damage from plant pests and diseases, particularly damping-off caused by Sclerotium rolfsii, which can lead to total plant death under severe infection. One promising, environmentally friendly, and cost-effective approach to disease management is the use of biological control agents such as Trichoderma spp., which function not only as biocontrol agents but also as biostimulants. This research was conducted at the Plant Pest and Disease Laboratory, Faculty of Agriculture Brawijaya University and Central Laboratory of BALITKABI for in vitro experiments and in vivo study located at a greenhouse of Agriculture Experimental Land Brawijaya University, Jatimulyo, Lowokwaru, Malang. A completely randomized design was used with five treatments and five replications. Applications of Trichoderma harzianum and T. asperellum, either individually or in combination, successfully reduced the incidence and severity of damping-off disease by 14% to 26.6% under field conditions. These treatments also increased the total phenolic content of the plants, indicating enhanced resistance, thus supporting the role of Trichoderma as an effective biocontrol agent. In addition, the combined application of Trichoderma species significantly increased the number of soybean leaves, indicating a biostimulant effect.
Identification of Bacterial Soft Rot in Potatoes From Batu City, Indonesia Berliana, Sonia; Abadi, Abdul Latief; Aini, Luqman Qurata; Yusnawan, Eriyanto
Agro Bali : Agricultural Journal Vol 8, No 3 (2025)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v8i3.2312

Abstract

Soft rot is a plant disease that can reduce both the quality and quantity of potato production, primarily caused by the Pectobacterium carotovorum. This study aims to isolate and identify the bacteria responsible for soft rot disease in potato tubers using molecular methods. Bacterial isolation was performed on a diseased potato tuber collected from Sumber Brantas Village, followed by a pathogenicity test, PCR, 16S rRNA gene sequencing, and phylogenetic analysis.  Based on macroscopic and microscopic examination, four isolates exhibiting soft rot symptoms were identified: P1, P2, K5, and K7. The 16S rRNA sequencing results and BLAST analysis showed that isolate K5 was closely related to Serratia rubidaea, isolate P2 was closely associated with Pectobacterium carotovorum, isolate P1 was closely related to Pseudomonas koreensis, and isolate K7 was closely associated with Ralstonia pseudosolanacearum. This study presents the first molecular characterization of bacteria responsible for soft rot in potato tubers originating from Batu City, East Java. The findings provide foundational insights that may support the development of molecular-based diagnostic tools and region-specific disease management strategies for potato cultivation in Indonesia.
Co-Authors Abdul Karim Fanani Achmad Baihaqi Achmad Noerkhaerin Putra Ageng Prayoga Wicaksono Ageng Prayoga Wicaksono Agung Wicaksono Ajeng Megasari Akhmad Rizali Alauddin, Mukhammad Wildan Albert Fernando Sitorus Alifia, Reva Yunisa Alvan Fajarudin Aminudin Afandhi Aminudin Afandhi Anak Agung Vini Nathasia Andhy Handoko Andhy Handoko Anggraeni Eka Puspitasari Anggraeni Eka Puspitasari Anis Rosyidah Anton Muhibbudin Anton Muhibuddin Ardiyan Taruna Ariffin Noor Sugiharto Ariffin Noor Sugiharto Arifin Noor Sugiharto Arifin Noor Sugiharto Asnawi Asnawi Asyifa, Ana Nabilah AthoÂ’illah Ahmad Athoillah Ahmad Auliya Zendhabad Aulya Retno Setyari Aulya Retno Setyari Baihaqi, Achmad Bambang Tri Rahardjo Bambang Tri Rahardjo Bambang Tri Raharjo Bambang Tri Raharjo Berliana, Sonia Bosha, Mohammed Chika Gianni Handayani Cokorda Javandira Cokorda Javandira Dewi, Fibrianti Shinta Dewi, Ovilya Kusuma Minarma Dianti, Sylvia Herli Didik Suprayogo Didik Suprayogo Dyah Ayu Kusuma Wardani Eka Kartini Eka Kartini Eko Famuji Ariyanto Eko Famuji Ariyanto Eriyanto Yusnawan Fery Abdul Choliq Firdausi, Wita Fitri Amaniyah Fuji Arriani, Intan Ganestya Indina Sari Ganestya Indina Sari Gatot Mudjiono Gatot Mudjiono Gatot Mudjiono Hagus Tarno Hakimah Halim Hanif, Luthfan Hardiana, Sindy Sukma Husna Fikriya Baroroh Ibrahim, Siti Ghia Azzahra Ichsan Firmansyah Ika Rochdjatun Sastrahidayat Ika Rochdjatun Sastrahidayat Indah Nur Khulillah Irisa Trianti Istiqomah Istiqomah Jantje Pelealu Jantje Pelealu Jasuli, Jasuli Kartika Try Rosanti Kartika Try Rosanti Kepel, Charles Kestrilia Rega Prilianti Khairunnisa', Amalia Khojin Supriadi Khojin Supriadi, Khojin Khotimah, Dwi Kusnul Kliwon Hidayat Liliek Sulistyowati Lilis Suryani Lilis Suryani Lilis Suryani Lilis suryani Luaili Addina LuÂ’aili Addina Luqman Qurata Aini Luqman Qurata Aini Luqman Qurata Aini Luqman Qurata Aini Luqman Qurota Aini Luqman Qurota Aini Ma'alifah, Nur Mahendra, Syah Maulana Maknunin, Lu'lu'il Marsya, Tia Indi Mayasari, Paulina Akita Meity Nelltje Tanor Mintarto Martosudiro Moch. Dawam Maghfoer Mochammad Nawawi Moh. Dawam Maghfoer Moh. Hibban Toana Muhammad Akhid Syib’li Muhammad Febriansyah Nawawi, Mochammad Novie Utami Asputri Novie Utami Asputri Nugroho Sulistyo Putro Nugroho Sulistyo Putro NURUL HIDAYAH Pamungkas, Bayu Aji Prabowo, Matheus Randy Pratama, Alifian Novaldi Satria Prillianti, Kestrillia Rega Putri, Rosy Cahyagung Dewi Basuki Qurata Aini, Luqman R. Sapto Hendri Boedi Soesatyo Ratri Kusuma Devi Ratri Kusuma Devi Rida Iswati Rifani Rusiana Dewi Rina Rachmawati Rudin, Sofyan Sudirman Ruth Stella Petrunella Thei S. Karindah Saidi, Aluna Uthilma Sama’ Iradat Tito Samharinto Samharinto Sektiono, Antok Wahyu Serdani, Army Dita Silfiani, Intan Silvi Ikawati Siska Irhamnawati Pulogu Soemarno Soemarno Sri Karindah Sri Karindah Sri Widyaningsih Sukamto, Ika Sumiyarsi Supriyono Supriyono Syamsuddin Djauhari Syarifah Maulidya Rahmah Syib'li, Muhammad Akhid Syib`'li, Muhammad Akhid Syib`li, Muhammad Akhid Syib’li, Muhammad Akhid Tambas, Jane Sulinda Tatiek Wardiyati Tatik Wardiyati Tatik Wardiyati Theresia Rani Kartika Ayu Toto Himawan Toto Himawan Trisnawati, Luh Putu Adi tubagus, satria wahyu Unun Triasih Vini Nathasia, Anak Agung Wardiyati, Tatiek Wayan Firdaus Mahmudy Wildanya Hafiah Wildanya Hafiah Wiwik Jatnika Wiwik Jatnika Wiwit Wicaksono Jati Yulianti, Novi Dwi Zainudin Zainudin Zainudin Zainudin Zainul Abidin Zainul Abidin