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RETRACTED : The Role of Trichoderma sp. as a Biocontrol Agent in Suppression of Root Rot Disease and Soybean Plant Biostimulants Maknunin, Lu'lu'il; Abadi, Abdul Latief; Choliq, Fery Abdul
Journal of Tropical Plant Protection Vol. 6 No. 1 (2025)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2025.006.1.2

Abstract

This article has been retracted at the request of the author. The author has published the article in the Jurnal Fitopatologi Indonesia (JFI).
Extreme Learning Machine Weight Optimization using Particle Swarm Optimization to Identify Sugar Cane Disease Alauddin, Mukhammad Wildan; Mahmudy, Wayan Firdaus; Abadi, Abdul Latief
Journal of Information Technology and Computer Science Vol. 4 No. 2: September 2019
Publisher : Faculty of Computer Science (FILKOM) Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1135.372 KB) | DOI: 10.25126/jitecs.201942116

Abstract

Sugar cane disease is a major factor in reducing sugar cane yields. The low intensity of experts to go into the field to check the condition of sugar cane causes the handling of sugarcane disease tends to be slow. This problem can be solved by instilling expert intelligence on sugar cane into an expert system. In this study the method of classification of sugar cane disease was proposed using Extreme Learning Machine (ELM). However, ELM alone is not enough to classify multilabel and multiclass disease case data in this study. Therefore, it is proposed to optimize the weight of hidden neurons in ELM using Particle Swarm Optimization (PSO). The experimental results show that the classification using ELM alone can reach an accuracy rate of 71%. After the weight of hidden neurons from ELM was optimized, the accuracy rate became 79.92% or an increase of 8.92%.
UJI EFEKTIVITAS BAHAN AKTIF FLUXAPYROXAD 50% + PYRACLOSTROBIN 50% DENGAN PENAMBAHAN AGENS HAYATI Bacillus subtilis TERHADAP PENYAKIT ANTRAKNOSA (Colletotrichum gloeosporioides) PADA TANAMAN CABAI RAWIT (Capsicum frutescens L.): EFFECTIVENESS TEST OF THE ACTIVE INGREDIENTS FLUXAPYROXAD 50% + PYRACLOSTROBIN 50% WITH THE ADDITION OF THE BIOLOGICAL AGENT Bacillus subtilis AGAINST ANTHRACNOSE DISEASE (Colletotrichum gloeosporioides) IN CAYENNE PEPPER (Capsicum frutescens L.) Asyifa, Ana Nabilah; Abadi, Abdul Latief
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 13 No. 1 (2025)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jurnalhpt.2025.013.1.3

Abstract

Penyakit antraknosa merupakan salah satu penyakit penting tanaman cabai rawit. Upaya pengendalian yang dilakukan yaitu menggunakan fungisida sintetik berbahan aktif fluxapyroxad 50% + pyraclostrobin 50%. Namun kedua bahan aktif tersebut memiliki spektrum luas sehingga berpotensi membunuh organisme non-target. Penelitian ini bertujuan untuk mengurangi dosis aplikasi fungisida sintetik dengan penambahan agens hayati Bacillus subtilis sebagai upaya menjaga keberlanjutan ekologi. Pengujian daya hambat dan kompatibilitas dilakukan secara in vitro, sementara aplikasi fungisida di lapang dilakukan menggunakan Rancangan Acak Kelompok (RAK) dengan 6 perlakuan dan 4 ulangan. Hasil penelitian menunjukkan bahan aktif fluxapyroxad + pyraclostrobin dapat menghambat jamur patogen C. gloeosporioides baik secara in vitro dan in vivo. Penambahan agens hayati B. subtilis dapat mengurangi penggunaan fungisida sintetik di lapang. Pengaplikasian kombinasi fungisida sintetik 75% dengan B. subtilis bersifat kompatibel dan efektif dalam menekan intensitas serangan penyakit antraknosa dengan tingkat efektivitas fungisida serta hasil panen yang tidak berbeda nyata dengan dosis rekomendasi.
Efficacy and Compatibility Test of Pseudomonas fluorescens with Fungicides Pyraclostrobin and Fluxapyroxad Against Alternaria solani in Tomato Plants Hardiana, Sindy Sukma; Abadi, Abdul Latief
Journal of Tropical Plant Protection Vol. 6 No. 2 (2025)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2025.006.2.5

Abstract

Early Blight disease is an important disease of tomato plants caused by the fungus Alternaria solani. This disease attack can cause a decrease in production yields up to 78%. Control efforts using synthetic fungicides continuously can cause new problems for the environment. This research was conducted to determine the effect of fungicide applications with active ingredients Pyraclostrobin+Fluxapyroxad and the addition of biological agent Pseudomonas fluorescens on early blight disease and growth of tomato plants. This study consisted of in vivo and in vitro studies with observation parameters in the form of compatibility of bacteria with fungicides, fungicide inhibition, disease intensity, level of efficacy, and growth of tomato plants. The results showed that the combination of synthetic fungicide and Pseudomonas fluorescens was classified as compatible and effective in controlling Alternaria solani and able to support the growth of tomato plants. Combination treatment can reduce the use of synthetic fungicide by 25% of the recommended dose and is able to give results that are not significantly different from the recommended dose of synthetic fungicide treatment.
ISOLASI DAN IDENTIFIKASI BAKTERI ENDOFIT DARI TANAMAN PADI (Oryza sativa) SEBAGAI PENGENDALI PENYAKIT HAWAR DAUN BAKTERI AKIBAT Xanthomonas oryzae pv. oryzae Serdani, Army Dita; Aini, Luqman Qurata; Abadi, Abdul Latief
Viabel : Jurnal Ilmiah Ilmu-Ilmu Pertanian Vol 12 No 1 (2018): Mei 2018
Publisher : Universitas Islam Balitar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (509.244 KB) | DOI: 10.35457/viabel.v12i1.422

Abstract

Rice cultivation often face obstacles, and one of them is bacterial leaf blight (BLB) disease caused by Xanthomonas oryzae pv. oryzae (Xoo). The application of endophytic bacteria is one of solutions to overcome this problem. Endophytic bacteria are non-pathogenic bacteria, which live in plant tissues. These bacteria could be isolated from the plant tissues. They may adapt to the plant tissues and produce antibiosis that could increase the plant resistance. Therefore, objectives of the research were to isolate the endophytic bacteria from healthy plant tissues and to identify them through morphology, physiology, biochemistry, and molecular. Method of the research was explorative along with three principal activities, such as isolation, selection, and identification on the potential endophytic bacteria. The isolation of the endophytic bacteria from healthy rice tissues has resulted 53 isolates and five of them have antagonistic ability in vitro against Xoo. Isolate ak9 has the highest antagonistic ability, 7.67 mm, in comparison with other isolates. Results of identification showed that those five potential bacteria have close relations, such as da3 with Bacillus cereus, isolate ak9 with Burkholderia sp., isolate ak30 with Enterobacter sp and isolate da9, as well as ak15 are Corynebacterium sp.
KARAKTERISASI BAKTERI PATOGEN PENYEBAB LAYU PADA TANAMAN BAWANG MERAH (Allium ascalonicum L.) Fuji Arriani, Intan; Latief Abadi, Abdul; Qurata Aini, Luqman
Viabel : Jurnal Ilmiah Ilmu-Ilmu Pertanian Vol 14 No 1 (2020): Mei 2020
Publisher : Universitas Islam Balitar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35457/viabel.v14i1.1004

Abstract

Shallot (Allium ascalonicum L.) is one of the vegetable commodities in the form of tubers that have a high economic value. The development of shallot cultivation in Indonesia often experiences obstacles, one of which is an obstacle in the process of shallot cultivation, namely the attack of Plant Disturbing Organisms (OPT). Information about diseases caused by pathogenic bacteria is still very limited. This study aims to determine the symptoms and identification of pathogenic bacteria that cause wilt in shallots. Bacterial isolation was carried out using Nutrient Agar (NA) media and 36 bacterial isolates were collected from shallots. Bacterial isolates were then tested for pathogenicity to determine the ability of bacteria to cause wilt disease in shallots. The results of isolation obtained 10 bacterial isolates that can show symptoms on red onions namely wilted leaves, yellow and soft rotten tubers. Four isolates including positive can show hypersensitivity symptoms, namely M11, N20, N17 and N14. Based on the identification of bacteria in physiology showed 2 groups of different isolates. Biochemical test results of Isolate M11, N20 and show species suspected of B. cepacia. N3 and N14 isolates are suspected to be E. carotavora subsp. carotavora. The bacterial isolates N7, N17, P5 and P7 were suspected to be E. carotavora subsp. betavasculorum. The isolate of N4 bacteria is suspected to be E. cacticida.
Potential of Indigenous Yeast as a Paraquat Biodegradation Agent in Sumberbrantas, Batu City, Indonesia Bosha, Mohammed; Abadi, Abdul Latief; Aini, Luqman Qurata
Journal of Tropical Plant Protection Vol. 3 No. 1 (2022)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2022.003.1.1

Abstract

Herbicides are a substance or chemical compound used to inhibit growth or kill plants. Herbicides are toxic to weeds or pests as well as cultivated plants. The impact of using the herbicide paraquat causes some microbes to become more resistant and has the potential to be used as a paraquat biodegradation agent. Indigenous yeast is one of the microbes that can be used as a biodegradation agent because it is resistant to the environment. For this reason, This research aimed to investigate the indigenous yeast isolated from the potato field in Sumberbrantas village, Batu City as a bioremediation agent for paraquat herbicide residues.The sampling was carried out in the potato field of Sumberbrantas, Batu City, which in routine maintenance uses a herbicide with active paraquat (1,1 dimethyl -4, 4-bipyridinium dichloride).The isolation results obtained 6 isolates of indigenous yeast which had the potential as biodegradation agents for paraquat. Treatment of yeast isolates MB 1, MB 3, MB 4, MB 6, MB 7, MB 8 at concentrations of 250, 500, 1,000 and 2,000 paraquat were able to survive with a growth percentage of 75-100%. At a concentration of 5,000 Isolated yeast decreased with a growth percentage of 50-75% and MB 1 isolate with 10,000 ppm concentration still survive with a growth percentage of 1-25%. The highest biodegradability ability of yeast was in MB 1. The identification of MB 1 isolates based on molecular characters have similarities in the basic structure of 98.89% with Geotrichum silvicola UFMG 354-228S isolates with accession number NG_0606221.1.
The Potential of Endophytic Fungi Producing Hydrolytic Enzymes as Biocontrol Agents of Alternaria porri Causing Onion Purple Blotch Firdausi, Wita; Abadi, Abdul Latief; Aini, Luqman Qurata; Martosudiro, Mintarto; Tarno, Hagus
AGRIVITA Journal of Agricultural Science Vol 47, No 3 (2025)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v47i3.4924

Abstract

Purple blotch caused by Alternaria porri is the primary disease in shallots, causing yield losses of 30-100%. The use of endophytic fungi as biocontrol agents has several advantages, including adaptability to host plants, selective targeting of pathogenic fungal cell walls without harming plant cells, and environmental friendliness. This study aims to select endophytic fungi producing hydrolytic enzymes with the highest inhibitory effect against A. porri. Endophytic fungi were isolated from the Batu Ijo local variety of shallots in Dau District, Malang, then evaluated for antagonism and for their ability to produce hydrolytic enzymes. This study obtained 42 isolates, consisting of 25 fungi and 17 yeasts. The endophytic fungi with the highest inhibitory activity were identified as Trichoderma asperellum (71.4%) and Pichia onychis (70.04%). T. asperellum produced cellulase at concentrations of 16.78 µg/ml and chitinase at 3.85 µg/ml, while P. onychis produced cellulase at concentrations of 11.47 µg/ml and chitinase at 1.56 µg/ml. Microscopic observation of A. porri hyphae showed that both endophytic fungi caused lysis with hyphal fragmentation, discoloration, and deformation. The endophytic fungi T. asperellum and P. onychis were proven to inhibit A. porri in vitro through their ability to produce hydrolytic enzymes that caused A. porri hyphal lysis.
Exploration of Pathogenic Microbes and Antagonistic Agents in Apple Production Centers in Malang Regency Abadi, Abdul Latief; Choliq, Fery Abdul; Trianti, Irisa; Maknunin, Lu'lu'il; Yulianti, Novi Dwi
Plantropica: Journal of Agricultural Science Vol. 9 No. 1 (2024)
Publisher : Department of Agronomy, Faculty of Agriculture, Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The apple farming group is one of the built of Department of Plant Pests and Diseases, Agricultural Faculty, Brawijaya University. In 2019, this departmen received a request from apple farmers in Madiredo Village, Pujon District to held an assistance programs of apple cultivation and environmentally pest control. This is related with the increasing of fruit rot and other diseases which is caused a decrease of apple production especially in Malang Regency. In general, apple farmers still rely on disease control using chemical fungicides which can have a negative impact on the environment and create resistance to pathogenic fungi. Therefore, this research was carried out to explore and study the potential of biological agents in apple production centers in Malang Regency, specifically in Poncokusumo, Pujon, and Batu District on conventional fields and mixed fields. This research was held observationally through fields surveys and farmer interviews, took soil samples and disease samples then then isolating, identifying and testing for antagonism at laboratory. Based on interviews and observation of symptomology, two dominant diseases were found, namely fruit rot disease (Gloeosporium sp.) and marsonina leaf spot disease (Marssonina coronaria). The results of exploration of the rhizospheric apple plants obtained isolates on conventional fields in Kec. Pujon has the highest abundance of fungal colonies compared to other regions. Based on the results of the antagonism test for pathogenic fungi and biological agents, it was found that the isolate codes KH.C, KK.A, JK.B, JK.J, and BK.B had the highest percentage of inhibition or the most effective in controlling the pathogen Gloeosporium sp. So in the next, the species can be identified molecularly.
In Vitro Analysis of Effectiveness Clove Leaf Extract (Syzygium aromaticum) Against Anthracnose Disease (Colletotrichum capsici) on Chili Plants (Capsicum annum L) Mahendra, Syah Maulana; Abadi, Abdul Latief; Choliq, Fery Abdul
Journal of Tropical Plant Protection Vol. 3 No. 2 (2022)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2022.003.2.4

Abstract

Chili (Capsicum annum L) is a plant with a high economic value and is in great demand by the Indonesian people. This research aimed to determine the effectiveness of the difference in the concentration of clove leaf extract in inhibiting anthracnose disease in chili. This research was conducted from February to April 2022. This research used the Complete Randomized Design (CRD) method with six treatments and four replications. The treatment used in this research was the difference in the concentration of clove leaf extract, namely 0%, 1%, 2%, 3%, 4%, and 5%. The research showed that clove leaf extract could inhibit the growth of C. capsici, which caused anthracnose disease in chili. In vitro tests showed that the concentrations of 3%, 4%, and 5% of clove leaf extract significantly differed from all treatments and had fungicidal properties against C. capsici. In conclusion, the research results showed that the effective concentration in inhibiting the growth of C. capsici, which causes anthracnose disease in chili, is the concentration of clove leaf extract 3%, 4%, and 5%.
Co-Authors Abdul Karim Fanani Achmad Baihaqi Achmad Noerkhaerin Putra Adi Setiawan Ageng Prayoga Wicaksono Ageng Prayoga Wicaksono Agung Wicaksono Ajeng Megasari Akhmad Rizali Alauddin, Mukhammad Wildan Albert Fernando Sitorus Alvan Fajarudin Aminudin Afandhi Aminudin Afandhi Anak Agung Vini Nathasia Andhy Handoko Andhy Handoko Anggraeni Eka Puspitasari Anggraeni Eka Puspitasari Anis Rosyidah Anton Muhibbudin Anton Muhibuddin Ardiyan Taruna Ariffin Noor Sugiharto Ariffin Noor Sugiharto Arifin Noor Sugiharto Arifin Noor Sugiharto Asnawi Asnawi Asyifa, Ana Nabilah AthoÂ’illah Ahmad Athoillah Ahmad Auliya Zendhabad Aulya Retno Setyari Aulya Retno Setyari Baihaqi, Achmad Bambang Tri Rahardjo Bambang Tri Rahardjo Bambang Tri Raharjo Bambang Tri Raharjo Berliana, Sonia Bosha, Mohammed Chika Gianni Handayani Cokorda Javandira Cokorda Javandira Dewi, Fibrianti Shinta Dewi, Ovilya Kusuma Minarma Dianti, Sylvia Herli Didik Suprayogo Didik Suprayogo Dyah Ayu Kusuma Wardani Eka Kartini Eka Kartini Eko Famuji Ariyanto Eko Famuji Ariyanto Eriyanto Yusnawan Fery Abdul Choliq Firdausi, Wita Fitri Amaniyah Fuji Arriani, Intan Ganestya Indina Sari Ganestya Indina Sari Gatot Mudjiono Gatot Mudjiono Gatot Mudjiono Hagus Tarno Hakimah Halim Hanif, Luthfan Hardiana, Sindy Sukma Husna Fikriya Baroroh Ibrahim, Siti Ghia Azzahra Ichsan Firmansyah Ika Rochdjatun Sastrahidayat Ika Rochdjatun Sastrahidayat Indah Nur Khulillah Irisa Trianti Istiqomah Istiqomah Jantje Pelealu Jantje Pelealu Jasuli, Jasuli Kartika Try Rosanti Kartika Try Rosanti Kestrilia Rega Prilianti Khairunnisa', Amalia Khojin Supriadi Khojin Supriadi, Khojin Khotimah, Dwi Kusnul Liliek Sulistyowati Lilis Suryani Lilis suryani Lilis Suryani Lilis Suryani Luaili Addina LuÂ’aili Addina Luqman Qurata Aini Luqman Qurata Aini Luqman Qurata Aini Luqman Qurata Aini Luqman Qurota Aini Luqman Qurota Aini Ma'alifah, Nur Mahendra, Syah Maulana Maknunin, Lu'lu'il Marsya, Tia Indi Mayasari, Paulina Akita Meity Nelltje Tanor Mintarto Martosudiro Moch. Dawam Maghfoer Mochammad Nawawi Moh. Dawam Maghfoer Moh. Hibban Toana Muhammad Akhid Syib’li Muhammad Febriansyah Nawawi, Mochammad Novie Utami Asputri Novie Utami Asputri Nugroho Sulistyo Putro Nugroho Sulistyo Putro NURUL HIDAYAH Pamungkas, Bayu Aji Prabowo, Matheus Randy Pratama, Alifian Novaldi Satria Prillianti, Kestrillia Rega Putri, Rosy Cahyagung Dewi Basuki Qurata Aini, Luqman R. Sapto Hendri Boedi Soesatyo Ratri Kusuma Devi Ratri Kusuma Devi Rida Iswati Rifani Rusiana Dewi Rina Rachmawati Rudin, Sofyan Sudirman Ruth Stella Petrunella Thei S. Karindah Saidi, Aluna Uthilma Sama’ Iradat Tito Samharinto Samharinto Sektiono, Antok Wahyu Serdani, Army Dita Silfiani, Intan Silvi Ikawati Siska Irhamnawati Pulogu Soemarno Soemarno Sri Karindah Sri Karindah Sri Widyaningsih Supriyono Supriyono Syamsuddin Djauhari Syarifah Maulidya Rahmah Syib'li, Muhammad Akhid Syib`'li, Muhammad Akhid Syib`li, Muhammad Akhid Syib’li, Muhammad Akhid Tatiek Wardiyati Tatik Wardiyati Tatik Wardiyati Theresia Rani Kartika Ayu Toto Himawan Toto Himawan Trisnawati, Luh Putu Adi tubagus, satria wahyu Unun Triasih Vini Nathasia, Anak Agung Wardiyati, Tatiek Wayan Firdaus Mahmudy Wildanya Hafiah Wildanya Hafiah Wiwik Jatnika Wiwik Jatnika Wiwit Wicaksono Jati Yulianti, Novi Dwi Zainudin Zainudin Zainudin Zainudin Zainul Abidin Zainul Abidin