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Isolasi dan Karakterisasi Potongan DNA Gen Sterol Metiltransferase 1 (SMT1) Asal Kelapa Sawit Rizal, Syamsi; Sukma, Dewi; Siberakuno, Roberdi; Liwang, Tony; Sudarsono
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 48 No. 3 (2020): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (611.715 KB) | DOI: 10.24831/jai.v48i3.32205

Abstract

Tanaman dengan pertambahan tinggi yang lambat merupakan karakter penting dalam program pemuliaan kelapa sawit dan brasinosteroid merupakan hormon penting yang berpengaruh untuk karakter tersebut. Gen sterol metiltransferase 1 (SMT1) merupakan salah satu gen kunci dalam biosintesis brasinosteroid. Pengembangan marker molekuler dapat dimulai dengan identifikasi dan karakterisasi gen yang terkait dengan karakter target. Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi keragaman nukleotida gen SMT1. Amplifikasi PCR dilakukan menggunakan genom Elaeis oleifera (E.o.), E. guineensis (E.g.) dan hibrida dari keduanya (E.g. x E.o.) dengan sepasang primer spesifik gen SMT1. Dalam penelitian ini berhasil diamplifikasi satu potongan DNA gen SMT1 dan dari hasil DNA sequencing berhasil diidentifikasi sebanyak 501- 505 pb. Potongan DNA yang diidentifikasi terdiri atas partial intron 8, exon 9, intron 9, dan patial exon 10, yang menyandi antara 104-105 residu asam amino. Domain residu asam amino terkonservasi ditemukan dalam hasil translasi amplikon, yang berkorelasi dengan situs aktif dalam biosintesis brasinosteroid dan mengkonfirmasi identitas amplikon sebagai bagian dari gen SMT1. Keragaman nukleotida potongan DNA gen SMT1 yang teridentifikasi berpotensi dapat digunakan untuk pengembangan marker molekuler yang bermanfaat untuk pemuliaan tanaman kelapa sawit, terutama untuk karakter pertambahan tinggi tanaman yang lambat. Kata kunci: Brasinosteroid, sterol, tanaman kerdil
Morphological traits and Zn content of several cassava genotypes in nutrient solution culture Pratama, Syilvia Nosya; Sudarsono; Ardie, Sintho Wahyuning; Sukma, Dewi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 51 No. 2 (2023): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/ija.v51i2.44535

Abstract

Leaf morphological characters and nutrient content in leaf tissue can vary depending on plant genotype. The nutrient solution culture can produce leaves with good nutritional content. Therefore, this research aimed to evaluate and validate cassava putative mutants' morphological traits and Zn content using a nutrient solution culture system. This research was carried out from January to May 2022 at the greenhouse of Cikabayan Experimental Garden, IPB University, Bogor. This study used two cassava cultivars and ten mutant genotypes from the cassava research team of IPB University, resulting from mutation breeding by gamma irradiation. The plant characters observed in this study included the number of leaves, length and width of the leaflet, leaf colour, plant height, fresh and dry weight leaf, and analysis of Zn content. The data were analyzed using ANOVA at a significant level of α = 5%, followed by Tukey's further test. The results showed that the G2D1-422 genotype had a higher Zn content than the wild type (Ratim/G2) and the other genotypes. The high Zn genotypes (G2D1-422) and low Zn genotypes (Ratim (G2) had not significantly different in fresh and dry leaf weight. Meanwhile, the plant genotype did not affect other growth characteristics such as plant height, the number of leaves, and the length and width of the leaflets, and overall, the characters were similar between the observed genotypes.
Pertumbuhan Tunas Temu Putih secara In Vitro pada Beberapa Komposisi Zat Pengatur Tumbuh Nazhira, Amalia; Dinarti, Diny; Sudarsono
Buletin Agrohorti Vol. 12 No. 2 (2024): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrob.v12i2.56863

Abstract

Rimpang jahe putih memiliki masa dormansi selama 2-3 bulan. Penelitian ini bertujuan untuk mengetahui komposisi zat pengatur tumbuh (ZPT) yang sesuai untuk perbanyakan tunas jahe putih secara in vitro. Penelitian dilaksanakan di Laboratorium Kultur Jaringan 3 AGH pada bulan Maret hingga Agustus 2022. Penelitian menggunakan rancangan acak lengkap pola faktorial (RAL) dengan 5 taraf komposisi ZPT pada media dasar MS, yaitu media MSo tanpa penambahan ZPT (kontrol); 3 ppm BAP + 0.5 ppm TDZ + 0.1 ppm NAA; 9 ppm BAP; 1.5 ppm TDZ; 3 ppm Kinetin + 0.5 ppm TDZ + 0.1 ppm NAA. Setiap perlakuan diulang sebanyak 5 kali, setiap unit percobaan terdiri dari 2 botol dan setiap botol ditanam satu eksplan. Penambahan 3 ppm BAP + 0.5 ppm TDZ + 0.1 ppm NAA dan 3 ppm Kinetin + 0.5 ppm TDZ + 0.1 ppm NAA memberikan hasil multiplikasi tunas yang paling tinggi, yaitu 6,8 tunas. Penambahan ZPT pada media tidak berpengaruh nyata terhadap pemanjangan tunas, pertambahan jumlah daun, dan pembentukan akar. Perlakuan dengan penambahan 3 ppm BAP + 0.5 ppm TDZ + 0.1 ppm NAA; 9 ppm BAP; dan 3 ppm Kinetin + 0.5 ppm TDZ + 0.1 ppm NAA menghasilkan plantlet yang menunjukkan kelangsungan hidup 100% pada masa aklimatisasi dua minggu. Kata kunci: auksin, multiplikasi, planlet, sitokinin, Zingiberaceae
PERBANDINGAN SPASIO-TEMPORAL KESEIMBANGAN LINGKUNGAN PLANKTON DI EMBUNG DAERAH BANTUL YOGYAKARTA Sudarsono; Ratnawati; Budiwati; Latifa, Annisa
Berita Biologi Vol 23 No 1 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2024.390

Abstract

Penelitian ini bertujuan untuk mengetahui perbandingan keseimbangan lingkungan dilihat dari komposisi jenis plankton, produktivitas primer, kelimpahan, indeks keanekaragaman, indeks dominansi dan indeks saprobik di Embung Potorono dan Embung Imogiri pada April dan Oktober 2021. Penelitian ini dilakukan pada bulan April 2021 sampai Juli 2022. Desain penelitian ini adalah deskriptif eksploratif. Pengambilan sampel dilakukan pada 5 stasiun dengan 5 pengulangan. Hasil penelitian menunjukkan bahwa kualitas perairan di keempat lokasi dan waktu termasuk dalam kategori tercemar ringan. Parameter yang berkontribusi terhadap kualitas air di Embung Imogiri dan Embung Potorono pada April 2021 adalah kedalaman air, curah hujan, dan suhu air yang tinggi. Curah hujan memiliki hubungan yang tidak signifikan dengan kelimpahan jenis yang tinggi namun melalui COD dapat dilihat adanya hubungan yang signifikan antara COD dengan kelimpahan jenis. Parameter yang berkontribusi terhadap kualitas air di Embung Imogiri pada Oktober 2021 adalah bahan organik, nitrat, DO, kekeruhan air dan magnesium yang cukup tinggi. Intensitas cahaya memiliki hubungan yang tidak signifikan dengan indeks dominansi fitoplankton namun nitrat, DO dan kekeruhan air memiliki hubungan yang signifikan dengan indeks dominansi fitoplankton. Parameter yang berkontribusi terhadap kualitas air di Embung Potorono pada Oktober 2021 adalah intensitas cahaya, BOD, COD, kalium dan fosfat. DO berpengaruh terhadap indeks keanekaragaman zooplankton yang dapat dilihat dari hubungan yang signifikan antara DO dengan indeks dominansi zooplankton.
Social Relations and Local Community Adaptation to the Presence of Mining Corporations in Banyuwangi Sudarsono; Bonaventura Ngw; Catur Wahyudi
International Journal of Education, Vocational and Social Science Vol. 4 No. 04 (2025): September - November, International Journal of Education, Vocational and Socia
Publisher : Cita konsultindo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63922/ijevss.v4i04.2510

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This study aims to analyze the dynamics of social relations and forms of adaptation of local communities to the presence of the mining company PT. Bumi Suksesindo (PT BSI) in Sumberagung Village, Pesanggaran District, Banyuwangi Regency. The presence of the gold mining industry has significantly changed the social, economic, and cultural structure of the community. This study uses a qualitative approach with a case study method, through data collection techniques in the form of field observations, in-depth interviews with the community, company officials, and government officials, as well as documentation. Data analysis was conducted interactively through the stages of data reduction, data presentation, and conclusion drawing. The results of the study show that the social relationship between the community and PT BSI is dynamic and complex, reflecting a process of negotiation between economic, social, and ecological interests. The community showed three main patterns of response to the presence of the mine: (1) cooperative, through involvement in corporate social responsibility (CSR) programs; (2) adaptive, by adjusting economic and social activities to changes in their living space; and (3) resistant, through rejection or criticism of the perceived environmental and social impacts. These patterns form a layered configuration of social relations between the community, the company, and the government, in which processes of accommodation and conflict occur simultaneously. This study concludes that the success of social relations between mining companies and local communities is highly dependent on two-way communication, policy transparency, and respect for the social values and local wisdom of the community. The implications of this study emphasize the importance of participatory social interaction models as the basis for equitable and sustainable community-corporate relationship management in mining areas
JUDICIAL LIMITS OF INTERVENTION IN ADMINISTRATIVE COURT Nugroho, Prasetyo; Shinta Hadiyantina; Sudarsono
International Journal of Educational Review, Law And Social Sciences (IJERLAS) Vol. 5 No. 6 (2025)
Publisher : CV. RADJA PUBLIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54443/ijerlas.v5i6.4222

Abstract

This journal aims to analyze the intervention process in dispute resolution in the Administrative Court. This study identifies two main problems: first, the mechanism for third-party entry into the dispute resolution process in the Administrative Court, and second, the legal consequences of third-party intervention in the case under study. The results of the study show that based on Article 83 of Law Number 5 of 1986 concerning Administrative Courts, third parties can enter the trial process during the examination, either on their own initiative, at the request of one of the parties, or at the initiative of the judge. The legal consequence of the entry of this third party is that they obtain the status of the Second Defendant of Intervention, which gives them the right to defend their interests in the trial process. This study emphasizes the importance of the presence of third parties in maintaining justice and legal certainty in state administrative disputes. The conclusion of this paper is that although intervention provides an opportunity for interested parties to participate in the legal process, there is a need for renewal and codification of the code of procedure in the Administrative Court to be more responsive to the dynamics of existing dispute resolution.
THE URGENCY OF FORCED MONEY REGULATION IN THE STATE ADMINISTRATIVE COURT: NORMATIVE ANALYSIS OF IMPLEMENTATION MECHANISMS Ferio Wabarca Hutabarat; Shinta Hadiyantina; Sudarsono
International Journal of Educational Review, Law And Social Sciences (IJERLAS) Vol. 5 No. 5 (2025)
Publisher : CV. RADJA PUBLIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54443/ijerlas.v5i6.4320

Abstract

The regulation of forced money (dwangsom) within the State Administrative Court is an important instrument to ensure compliance of state administrative officials with court decisions that have permanent legal force. However, until now the regulations governing the mechanism for the implementation of forced money in the PTUN are still unclear, so it often causes obstacles in the practice of execution. This study aims to analyze the urgency of the regulation of forced money in the State Administrative Court by focusing on the implementation mechanism so that it can be implemented effectively. The research method used is a prescriptive method with a normative approach, namely examining applicable laws and regulations, legal doctrines, and relevant court decisions. The results of the study show that the absence of strict regulations regarding the implementation of forced money creates legal uncertainty and has the potential to weaken the function of the PTUN as judicial control over government administrative actions. Therefore, a more comprehensive regulation is needed regarding the mechanisms, procedures, and parameters of the implementation of forced money in order to provide legal certainty, ensure the effectiveness of the execution of decisions, and strengthen the principle of the rule of law in the administration of government.
PENDEKATAN HUMANISTIK DALAM PEMBELAJARAN UNTUK MENGEMBANGKAN POTENSI PESERTA DIDIK DI MI NAILUL HUDA JIMBARAN-BADUNG, BALI Katmani; Sudarsono
Pendas : Jurnal Ilmiah Pendidikan Dasar Vol. 10 No. 04 (2025): Volume 10 No. 04 Desember 2025 Terbit
Publisher : Program Studi Pendidikan Guru Sekolah Dasar FKIP Universitas Pasundan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23969/jp.v10i04.35938

Abstract

This study aims to analyze the practices and impact of a humanistic approach in learning to develop the potential of students at MI Nailul Huda, Jimbaran-Badung. The humanistic approach, which emphasizes respect for the individual, is considered relevant to the principles of Islamic education. This qualitative research utilized a Focus Group Discussion (FGD) method with five teachers from MI Nailul Huda serving as subjects. The data were analyzed thematically to identify the understanding, practices, and impact of this approach. The findings indicate that the implementation of the humanistic approach successfully created a positive learning environment, strengthened teacher-student interpersonal relationships, and significantly enhanced students' self-confidence, creativity, and empathy. The study found that this approach aligns with the concept of fitrah (human nature) in Islam and the educational vision proposed by al-Ghazali. However, challenges such as a dense curriculum and cognitive-oriented assessment remain major obstacles. The research recommends continuous training to strengthen the consistent implementation of this approach.
Business Licensing Obligations for E-Commerce under Government Regulation No. 80/2019 Achmad Haekal; Sudarsono; Shinta Hadiyantina
YURISDIKSI : Jurnal Wacana Hukum dan Sains Vol. 21 No. 3 (2025): December
Publisher : Faculty of Law, Merdeka University Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55173/yurisdiksi.v21i3.330

Abstract

This study examines the discrepancy between das sollen and das sein regarding the obligation to register a business license as mandated in Article 15(1) of Government Regulation Number 80 of 2019. Although the regulation requires all business actors to register their licenses with the Central Government, its implementation in Malang City remains ineffective. Of approximately 85,200 MSMEs, only about 22,000 had obtained official licenses by 2024. The study addresses two issues: (1) the factors contributing to the low compliance of micro-enterprise actors with licensing requirements, and (2) the legal implications for those operating without a business license. Using a socio-legal method with a juridical-sociological approach, the research relies on primary and secondary data collected through fieldwork and literature study. The sample consists of 13 respondents and 2 key informants, analyzed qualitatively. The findings reveal that the main cause of ineffective implementation is the low level of legal awareness and knowledge among micro-enterprise actors. Eight respondents were unaware of the obligation to register with the Disnaker PMPTSP of Malang City, while five respondents were aware but had not complied. This demonstrates that regulatory implementation has not been optimal. The legal implications for non-compliant business actors include the loss of legal validity of their operations and the potential imposition of administrative sanctions under applicable laws.
Desain dan Pembuatan Pemanggang Biji Kopi untuk Masyarakat Petani Kopi Desa Rejoagung Sumberwringin Bondowoso M. Arief Bustomi; Bachtera Indarto; Sudarsono; Faridawati; Eko Minarto
Sewagati Vol 9 No 5 (2025)
Publisher : Pusat Publikasi ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j26139960.v9i5.9044

Abstract

Kabupaten Bondowoso merupakan penghasil kopi robusta dan arabika di JawaTimur dengan areal perkebunan yang didominasi perkebunan rakyat. Meskipunproduksi kopi di Bondowoso cukup tinggi, pengolahan pascapanen kopi masihdilakukan secara tradisional, sehingga menyebabkan nilai tambah kopi dan produktivitaspetani belum maksimal. Tujuan kegiatan pengabdian masyarakat ini adalahuntuk meningkatkan nilai tambah kopi pasca panen dengan mesin pemanggangbiji kopi. Mesin ini dirancang dengan kontrol otomatis untuk mendeteksi tingkatkematangan biji kopi dengan baik. Sistem ini memungkinkan pengaturan suhu optimal,sehingga menghasilkan kualitas pemanggangan yang konsisten dan seragam.Dalam kegiatan pengabdian masyarakat ini, keunggulan mesin pemanggang bijikopi karya penelitian para dosen dan mahasiswa Fisika ITS diterapkan di desaRejoagung kecamatan Sumberwringin kabupaten Bondowoso. Pelaksanaan kegiatanpengabdian masyarakat di desa Rejoagung adalah kunjungan pertama akhir Juli 2025untuk survey dan sosialisasi ke petani. Kunjungan kedua akhir Agustus 2025 untukmembawa dan memasang peralatan pemanggang biji kopi. Kunjungan ketiga akhirSeptember 2025 untuk memberikan pelatihan penggunaan dan perawatan peralatan.Metode kegiatan pengabdian berupa pemberian peralatan pemanggang biji kopi danpelatihan penggunaan dan pemeliharaannya kepada petani. Hasil dari pelaksanaankegiatan pengabdian ini adalah peningkatan nilai tambah produk kopi desa Rejoagungdengan pemanfaatan mesin pemangggang biji kopi.
Co-Authors Aan Eko Widiarto Abdul Madjid Abdurahman Achmad Afandi, Achmad Achmad Haekal Agus Purwito Aisyah, S. I. Amadhan Takwir Aminur Andi Asda Astiah Annisa Latifa Asmuni Auliza, Nur Awalia, Rizki Novita Azahra, B. S. Bachtera Indarto BAMBANG SUGIRI Biahimo, Suci Ramadani I Bonaventura Ngw Budiman Sudia, Budiman Budiwati Bunau , Eusabinus Caecilia Pujiastuti Cahyoginarti, Cahyoginarti Catur Wahyudi Cyrilla Indri Parwati Darminto Darwanto David Pasaribu Demon, Fransiskus Dominikus Dewi Ratna Muchlisa Mandyara Dewi Sukma Diky Anggoro, Diky Diny Dinarti Dita Aditianingsih Dunggio, Titin Dwi Riyanti Efendi, Aan Eka Fajar Rahmani Eko Minarto Elizabeth Handini Endang Susilawati Eshan Ratu Azzahra Eusabinus Bunau Faridawati Fathurrahman Muhtar Ferio Wabarca Hutabarat Firihu, Muhammad Zamrun Hanifah, Lulu Siti Haniffudin Nurdiansah Hardianto, D. Harsini Hasniza Nordin Husain, Fitriyani Idrus, Iska Pramishella Idrus, Iska Pramishella Idrus Ika Roostika Ikhsanudin Imam Mashudi Indah Dwi Qurbani Irma Nurjannah, Irma Istislam Iwa Sutardjo Jenrison Nainggolan Katmani Kholil , Ahmad Komang Alit Antara L. Abdullah La Hasanudin Lisa Ma'rufi Lubis, Uchti Nuzul Qhinanti Luwandi Suhartono M. Arief Bustomi M. Salman Alfarisy Maat, Siti Mistima Mawaddah, Ida Mira Humaira Mochamad Zainuri Moh. Fadli Mohamad Abdun Nasir Mubarok, Fahmi Muhammad Luthfi Muhammad Yusuf Muhammad Zuardi Mushofi, Yuskhil Nazhira, Amalia Negoro, P. S. Neneng Aminah Noe, Ismiyati A Noho, Nur Novianti Nopri Esmiralda Nurrahmantyo, Guntang Palu, Raphael Putra Patamani, Flora Prasetyo Nugroho Pratama, Syilvia Nosya Pratiwi, Della Anugrah Puja Kharisma Permata Ayu Purba, R. D. Purwoko, Agus Puti Almas Raden Rinova Sisworo Rahayu Apriliaswati Rahmayanti RATNAWATI Regina resliana Retno Asih, Retno Riban Satia, Muhammad Ridway Balaka, Ridway Rini Nuraini Rizal, Syamsi Royani, J. I. Samhuddin Shandra Amarillis Shinta Hadiyantina Siberakuno, Roberdi Silfiana Sintho Wahyuning Ardie Siti Faridah Siti Sunarintyas Suasmoro Sudibyo Martono Suhartono , Luwandi Sukma Sahreny SUMARNI Suminar Pratapa Supaino Susilawati , Endang Sutapa, Yohanes Gatot Syarif Husin Taufik, Beatrice Ellen Tony Liwang Triwikantoro Viska Inda Variani Wahyudi, Edi Wardah WINARNO Yanti Sri Rejeki Yosef p. Koton Yuliani, Ummi Yurnidarsyah, Citra