Article Per Year (5 Year)
p-Index From 2021 - 2026
2.108
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Jurnal Pengolahan Hasil Perikanan Indonesia Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Ilmu Dasar Jurnal Sumberdaya Hayati Journal of Multidisciplinary Applied Natural Science Molekul: Jurnal Ilmiah Kimia Berita Biologi
Primahana, Gian
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Physics

Published : 15 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 15 Documents
Search

 1   2 
Antioxidant, Anti-Aging and Antibacterial Activity from Dewa Leaves Ethanolic Extract (Gynura japonica (Thunb.) Juel) Fatmawati, Umi; Zienitha, Anggie Meilinda; Anggraini, Meisi; Rosita, Lisa; Indrowati, Meti; Harlita, Harlita; Santosa, Slamet; Permatasari, Vera; Primahana, Gian; Yati, Indri; Prastya, Muhamad Eka
Molekul Vol 20 No 2 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.2.13792

Abstract

ABSTRACT. Due to their phytochemical constituents, Dewa leaves (Gynura japonica (Thunb.) Juel.) are often used in traditional medicinal herbs. However, in vitro and in vivo of antioxidative and anti-aging studies of Dewa leaves on yeast as a eukaryotic cell model have not been widely carried out. This study aims to determine the antioxidant, anti-aging, and antibacterial activities derived from G. japonica leaves extract. Extraction was conducted using 70% and 96% ethanol solvents, total phenolic content (TPC) was assayed using Folin-Ciocalteu method, flavonoid contents (TFC) was assayed using aluminum chloride method, antioxidant activity was tested using DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2-azinobis-3-Ethylbenzothiazoline)-6-sulfonic acid) radicals. Subsequently, an anti-aging activity test was performed on the yeast Saccharomyces cerevisiae as a model organism following antibacterial activity. The antibacterial activity test was carried out using well diffusion agar, and the Minimal Inhibitory Concentration (MIC) was determined using the microplate method. We obtained that G. japonica leaves extracted from 70% and 96% methanol solvents, have TPC of 13.14 and 22.11 mg GAE/gr extract, and TFC of 8.04 and 14.09 mg QE/gr extract, respectively. As for DPPH and ABTS antioxidant activity, D70 showed the best activity with IC50 values of 1411.36±56.35 µg/mL and 2516.10±18.77 µg/mL, respectively. The anti-aging test showed that both 70% and 96% ethanol extracts were able to maintain the yeast cell viability under H2O2 oxidative stress. Further, 70% and 96% ethanol extract also showed antibacterial activity at the best value against Staphylococcus aureus with a MIC value of 390.62 µg/mL, it means that Gynura leaves extract has a potency as antibacterial agent. The results of this study indicate that the ethanol extract of G. japonica leaves can be developed for further investigation as an antioxidant and antibacterial therapeutic agent. Keywords: Anti-aging, antibacterial, antioxidant, Gynura japonica, Saccharomyces cerevisiae
Dammarane-Type Triterpenoids from Twigs of Aglaia Foveolata and Their Antibacterial Activity Hutagaol, Ricson Pemimpin; Mozef, Tjandrawati; Nurilmala, Febi; Primahana, Gian; Fajriah, Sofa; Prastya, Muhammad Eka; Hidayatullah, Sigit; Satyaningsih, Desy
Molekul Vol 20 No 2 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.2.16241

Abstract

ABSTRACT. The Aglaia species, which contains triterpenoids, is the most numerous in the Meliaceae family. Aglaia foveolata (A. foveolata) is a type of plant that has many benefits, as medicinal ingredients. The potential of this plant is inseparable from the content of various bioactive compounds. This study aims to isolate, characterize the active compound from the twigs of A. foveolata and test its activity as an antibacterial. Three dammarane-type triterpenoids were isolated from the A. foveolata twigs which is, namely dammar-24-en-3β,20-diol (1), an epimeric mixture of shoreic and eichlerianic acid (2, 3). Their chemical structures were determined based on spectroscopic data using infrared, high-resolution mass spectrometry, and including one and two-dimensional NMR techniques, as well as through data comparison of the reported compound. Compound 1 was reported for the first time to be successfully isolated from this species. All these substances were tested for the first time for their antibacterial activity against two Gram-positive bacteria Staphylococcus aureus and Bacillus subtilis and two Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa, through this study. Compound 1 was inactive, the epimeric mixture of 2 and 3 showed moderate antibacterial activity with a minimum inhibitory concentration (MIC) value ranging from 31.7 to 126.6 ppm, particularly against S. aureus with a MIC value of 31.7 ppm. Keywords: Aglaia, Elucidation, Isolation, Spectroscopy, Bacterial
Evaluasi Aktivitas Antioksidan dan Antibakteri Dari Daun Empat Tumbuhan Suku Baduy Sebagai Sumber Bahan Alam Potensial Permatasari, Vera; Riskiana, Angger Arfi; Saefurohman, Asep; Prastya, Muhammad Eka; Ridha, Rafifa; Primahana, Gian; Dewijanti, Indah Dwiatmi
Jurnal Sumberdaya Hayati Vol. 11 No. 3 (2025): 2025
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.11.3.122-129

Abstract

Baduy indigenous plants have great potential as natural sources of bioactive compounds, yet scientific studies on their biological activities remain limited. This study aimed to evaluate the antioxidant, antibacterial activities, and total phenolic content of the leaves from four Baduy plant species: Gardenia jasminoides, Strobilanthes crispus, Ficus callosa, and Artocarpus camansi. The leaves were extracted with methanol, followed by fractionation using n-hexane and ethyl acetate. Antioxidant activity was assessed using DPPH and ABTS assays, total phenolic content was determined by the Folin–Ciocalteu method, and antibacterial activity was tested against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, and Escherichia coli using the broth dilution method to determine Minimum Inhibitory Concentration (MIC). The results showed that the ethyl acetate fraction of A. camansi leaves exhibited the highest total phenolic content (23.53 mg GAE/g extract). This fraction demonstrated strong antioxidant activity in the ABTS assay (IC50 = 18.55 ppm) and moderate activity in the DPPH assay (IC50 = 50.67 ppm). Moreover, it showed strong antibacterial activity against S. aureus, B. subtilis, and P. aeruginosa. These findings indicate that A. camansi leaves possess promising potential as a natural source of antioxidant and antibacterial agents.
Aktivitas Antibakteri dan Antioksidan dari Senyawa Bioaktif Asal Bakteri Endofit Tanaman Nyatoh (Palaquium amboinense B.) : Antibacterial and Antioxidant Properties of Bioactive Compounds Derived from Endophytic Bacteria Isolated from Nyatoh (Palaquium amboinense B.) Putra, Rayhan Helmyana; Retnowati, Dwi; Cordova, Datu Muhammad; Zahra, Aliya Azkia; Primahana, Gian; Dewi, Rizna Triana; Filailla, Euis; Sukirno; Prastya, Muhammad Eka
Jurnal Sumberdaya Hayati Vol. 10 No. 1 (2024): 2024
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.10.1.25-32

Abstract

Nyatoh plant (Palaquium amboinense B.) is native to Indonesia which is known to have the ability to produce potential secondary metabolites. The aim of this study was to evaluate the antibacterial and antioxidant activities from bioactive compounds produced by endophytic bacteria from nyatoh leaves. Five endophytic bacteria were tested against 4 targeted bacteria including Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 15442, Bacillus subtilis ATCC 19659, and Staphylococcus aureus ATCC 6538. Bacterial isolate which had the strongest antibacterial activity was then fermented and extracted. The corresponding extract was further tested on disc diffusion method, determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values, antioxidant evaluation, and analyzed for its bioactive compound components via GC-MS analysis. The results showed that one isolate, namely D1 can produce secondary metabolite which act as antibacterial and antioxidant potentials. This corresponding extract has the strongest MIC and MBC against B. subtilis with value of 39.5 and >78.1 µg/ml and exhibited antioxidant activity against 2,2-difenil-1-pikrilhidrazil (DPPH) with inhibition value of 85.37%. Based on GC-MS analysis, secondary metabolites derived from this potential extract contained some major active constituents including Tributyl acetylcitrate and bis (2-ethylhexyl) ester which might act as antibacterial and antioxidant agents.
ISOLATION AND IDENTIFICATION OF APIGENIN, A FLAVONOID COMPOUND FROM MACARANGA HYPOLEUCA (REICHB.F. & ZOLL.) Aisya, Sitti; Megawati, Megawati; Ariani, Novita; Sukirno, Sukirno; Minarti, Minarti; Kurniawan, Hendris Hendarsyah; Hidayat, Arief; Hendra, Medi; Primahana, Gian; Darmawan, Akhmad
Berita Biologi Vol 23 No 1 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2024.3242

Abstract

The study on Macarang hypolueca (Reichb.f. & Zoll.), which was collected from secondary forests around Samarinda City, East Kalimantan, involved phytochemical investigations that led to the isolation of a flavone type compound of flavonoid from the ethyl acetate fraction. To separate the compounds, silica gel column chromatography was utilized with a gradient solvent system of n-hexane and ethyl acetate, along with the addition of 5%. Infrared analysis (FTIR), mass spectrum (LC-ESI-MS), and nuclear magnetic resonance (1D- and 2D-NMR) were used to identify and elucidate the structure. Based on spectroscopic data and comparison with appropriate references, the isolated compound was identified as apigenin
Co-Authors A.A. Ketut Agung Cahyawan W Abda Abda Ace Tatang Hidayat Ahmad Randy Aisya, Sitti Akhmad Darmawan Anggraini, Meisi Ariani, Novita Asep Saefurohman Atwita, Syelziva Yonda Barokah, Giri Rohmad Chang, Helen Cordova, Datu Muhammad Desi Harneti Putri Huspa Dewijanti, Indah D. Dewijanti, Indah Dwiatmi Dwi Retnowati Eti Nurwening Sholikhah Febi Nurilmala Fikriyah, Annisaa Nurul Filailla, Euis Harlita Harlita Hasidu, La Ode Abdul Fajar Hendris Hendarsyah Kurniawan, Hendris Hendarsyah Hening, Egiyanti Nur Widhia Hidayatullah, Sigit Jekmal Malau Jepri Agung Priyanto, Jepri Agung Khalijah Awang Linar Zalinar Udin, Linar Zalinar Maulidiyah Medi Hendra Megawati Megawati - Meti Indrowati Minarti Minarti Muhammad Nurdin N. Nurjanah Permatasari, Vera Prastya, Muhamad Eka Prastya, Muhammad Eka Pulung, Maria Ludya Putra, Rayhan Helmyana Respati Tri Swasono Rhesi Kristiana Ricson Pemimpin Hutagaol Ridha, Rafifa Riskiana, Angger Arfi Rizna Triana Dewi Rosita, Lisa Rosmalena, Rosmalena Satyaningsih, Desy Shiono, Yoshihito Simbolon, Sumihartati Slamet Santosa Sofa Fajriah Sri Pujiyanto SUKIRNO Sukirno Sukirno Suryanti, Erma TATI NURHAYATI Tjandrawati Mozef Tri Joko Raharjo UMI FATMAWATI Unang Supratman Vivitri Dewi Prasasty yati, indri Yogaswara, Radite Yuswan, Apriza Zahra, Aliya Azkia Zienitha, Anggie Meilinda