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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Pengolahan Hasil Perikanan Indonesia Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Ilmu Dasar Jurnal Sumberdaya Hayati JSFK (Jurnal Sains Farmasi & Klinis) Journal of Multidisciplinary Applied Natural Science Journal Of World Science Molekul: Jurnal Ilmiah Kimia Berita Biologi
Primahana, Gian
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Energy Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences

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Metabolomic and Cytotoxicity Profiles of Ethanol Extract of Peronema canescens Jack on Human Non-small Lung Cancer Cell A549 Anjayani, Nuri; Amalia, Senya Putri; Sukmarini, Linda; Tamhid, Hady Anshory; Primahana, Gian; Triastuti, Asih
JSFK (Jurnal Sains Farmasi & Klinis) Vol 12 No 1 (2025): J Sains Farm Klin 12(1), April 2025
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jsfk.12.1.7-14.2025

Abstract

Cancer has a high prevalence and mortality in the world. Cancer treatment is still hampered by high levels of side effects, drug resistance and the lack of affordable prices for anticancer drugs. It is necessary to develop new anticancer drugs to help overcome this problem. One of the plants that has the potential to be developed as an anticancer drug is Sungkai (Peronema canescens Jack). This study aims to determine the cytotoxic activity of sungkai leaf and identify its metabolomic profile. The sungkai leaves were macerated for 24 hours with 96% ethanol. Metabolomic profiles were analyzed with Ultra High-Performance Liquid Chromatography-High Resolution Mass Spectrometry (UHPLC-HRMS). Chemical structure identification was performed by MS-DIAL and MS-FINDER platforms. The ethanol extract of sungkai leaves was analyzed for its cytotoxic activity using the MTT test on A549 lung cancer cells and its selectivity on normal HDFa fibroblast cells. The ethanol extract of sungkai showed IC50 105,21 µg/mL on A549 cells and no cytotoxic activity against normal HDFa cells. Based on the metabolomic analysis, 7 furano terpenoid compounds were detected in the ethanol extract, namely peronemin A2; A3; B1; B2; B3: C1; and D1 along with other compounds. In conclusion, the ethanol extract of P. canescens leaves has cytotoxic and selective activity against A549 lung cancer cells, and potential to be further developed as an anticancer drug candidate. Peronemins and other substances like flavonoids and polyphenols may be linked to the cytotoxic properties of sungkai leaves
Cytotoxic of Usnic Acid Isolated from Ramalina sp.: Cytotoxic of Usnic Acid Isolated from Ramalina sp. Darmawan, Akhmad; Maulidiyah; Megawati; Ariani, Novita; Aisya, Sitti; Sukirno; Randy, Ahmad; Primahana, Gian; Hendra, Medi; Nurdin, Muhammad
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.14

Abstract

Ramalina sp. (Ramalinaceae) is a type of lichen known to contain many active secondary metabolite compounds that have the potential to be used as medicine or medicinal raw materials. One of the biological activities possessed by Ramalina sp. lichen is its anticancer activity. This research aims to isolate and identify active secondary metabolite compounds from the methanol extract of the Ramalina sp. lichen and to find out the cytotoxic activity of the isolated compound against MCF7 breast cancer cells. Compound 1 (usnic acid) was successfully isolated from fraction A. The isolation and purification process was carried out starting with a maceration process using acetone solvent, followed by silica gel column chromatography using a gradient solvent system consisting of n-hexane, n-hexane:EtOAc, EtOAc, EtOAc:MeOH, and MeOH with 5% increment of polarity to obtain 17 fractions (F-1 to F-17). From the 17 fractions obtained, fraction 3 (F-3) and fraction 4 (F-4) (eluted with n-hexane:EtOAc 30%), which had the same TLC profile, were combined and named as fraction A. Compound 1 (50 mg) is a yellow needle crystal that was formed in a bottle of fraction A, which was obtained after the process of combining fractions F-3 and F-4 and solvent evaporation process. The crystals were then separated and purified with CHCl3 and MeOH. Compound 1 was then characterized based on spectroscopic data. Various spectroscopic analysis data, including FTIR, 1D- and 2D-NMR, and LC-ESI-MS, show that Compound 1 is a dibenzofuran derivative compound with 18 carbons (3 from carbonyl groups (C=O) and 3 from methyl groups) and 2 hydroxyl (-OH). Cytotoxicity assay showed that at a low concentration of 18.75 ug/mL, Compound 1 caused a 67.06% decrease in MCF7 viability
Antioxidant, Anti-Aging and Antibacterial Activity from Dewa Leaves Ethanolic Extract (Gynura japonica (Thunb.) Juel) Fatmawati, Umi; Zienitha, Anggie Meilinda; Anggraini, Meisi; Rosita, Lisa; Indrowati, Meti; Harlita, Harlita; Santosa, Slamet; Permatasari, Vera; Primahana, Gian; Yati, Indri; Prastya, Muhamad Eka
Molekul Vol 20 No 2 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.2.13792

Abstract

ABSTRACT. Due to their phytochemical constituents, Dewa leaves (Gynura japonica (Thunb.) Juel.) are often used in traditional medicinal herbs. However, in vitro and in vivo of antioxidative and anti-aging studies of Dewa leaves on yeast as a eukaryotic cell model have not been widely carried out. This study aims to determine the antioxidant, anti-aging, and antibacterial activities derived from G. japonica leaves extract. Extraction was conducted using 70% and 96% ethanol solvents, total phenolic content (TPC) was assayed using Folin-Ciocalteu method, flavonoid contents (TFC) was assayed using aluminum chloride method, antioxidant activity was tested using DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2-azinobis-3-Ethylbenzothiazoline)-6-sulfonic acid) radicals. Subsequently, an anti-aging activity test was performed on the yeast Saccharomyces cerevisiae as a model organism following antibacterial activity. The antibacterial activity test was carried out using well diffusion agar, and the Minimal Inhibitory Concentration (MIC) was determined using the microplate method. We obtained that G. japonica leaves extracted from 70% and 96% methanol solvents, have TPC of 13.14 and 22.11 mg GAE/gr extract, and TFC of 8.04 and 14.09 mg QE/gr extract, respectively. As for DPPH and ABTS antioxidant activity, D70 showed the best activity with IC50 values of 1411.36±56.35 µg/mL and 2516.10±18.77 µg/mL, respectively. The anti-aging test showed that both 70% and 96% ethanol extracts were able to maintain the yeast cell viability under H2O2 oxidative stress. Further, 70% and 96% ethanol extract also showed antibacterial activity at the best value against Staphylococcus aureus with a MIC value of 390.62 µg/mL, it means that Gynura leaves extract has a potency as antibacterial agent. The results of this study indicate that the ethanol extract of G. japonica leaves can be developed for further investigation as an antioxidant and antibacterial therapeutic agent. Keywords: Anti-aging, antibacterial, antioxidant, Gynura japonica, Saccharomyces cerevisiae
Dammarane-Type Triterpenoids from Twigs of Aglaia Foveolata and Their Antibacterial Activity Hutagaol, Ricson Pemimpin; Mozef, Tjandrawati; Nurilmala, Febi; Primahana, Gian; Fajriah, Sofa; Prastya, Muhammad Eka; Hidayatullah, Sigit; Satyaningsih, Desy
Molekul Vol 20 No 2 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.2.16241

Abstract

ABSTRACT. The Aglaia species, which contains triterpenoids, is the most numerous in the Meliaceae family. Aglaia foveolata (A. foveolata) is a type of plant that has many benefits, as medicinal ingredients. The potential of this plant is inseparable from the content of various bioactive compounds. This study aims to isolate, characterize the active compound from the twigs of A. foveolata and test its activity as an antibacterial. Three dammarane-type triterpenoids were isolated from the A. foveolata twigs which is, namely dammar-24-en-3β,20-diol (1), an epimeric mixture of shoreic and eichlerianic acid (2, 3). Their chemical structures were determined based on spectroscopic data using infrared, high-resolution mass spectrometry, and including one and two-dimensional NMR techniques, as well as through data comparison of the reported compound. Compound 1 was reported for the first time to be successfully isolated from this species. All these substances were tested for the first time for their antibacterial activity against two Gram-positive bacteria Staphylococcus aureus and Bacillus subtilis and two Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa, through this study. Compound 1 was inactive, the epimeric mixture of 2 and 3 showed moderate antibacterial activity with a minimum inhibitory concentration (MIC) value ranging from 31.7 to 126.6 ppm, particularly against S. aureus with a MIC value of 31.7 ppm. Keywords: Aglaia, Elucidation, Isolation, Spectroscopy, Bacterial
Exploration and Antimicrobial Activity Test of Secondary Metabolites from Endophytic Fungi of Curcuma Zedoaria from West Nusa Tenggara Mario, Mario; Rizky Prima, Sylvia; Primahana, Gian
Journal of World Science Vol. 4 No. 9 (2025): Journal of World Science
Publisher : Riviera Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58344/jws.v4i9.1505

Abstract

Antibiotic resistance has become a significant public health issue in Indonesia, prompting the search for alternative antimicrobial agents from natural sources. Endophytic fungi symbiotically living inside medicinal plants such as Curcuma zedoaria (temu putih) have the potential to produce bioactive secondary metabolites that could serve as new antibacterial agents. This study aimed to isolate endophytic fungi from the rhizome of temu putih originating from West Nusa Tenggara (NTB), evaluate the antibacterial activity of isolates grown on Potato Dextrose Broth (PDB) and Yeast Malt Broth (YMB), and identify as well as elucidate the structure of the secondary metabolite compounds produced. This experimental comparative study involved isolation of endophytic fungi by surface sterilization, culturing on PDB and YMB media, extraction with ethyl acetate, and antibacterial testing against Staphylococcus aureus ATCC 6538 and Escherichia coli ATCC 8739 using the microdilution method. Metabolite profiling was performed using HPLC-DAD, preparative HPLC for fractionation, LC-HRMS for molecular mass identification, and NMR spectroscopy for structural elucidation. Five endophytic fungal isolates were successfully obtained from the temu putih rhizome. Antibacterial activity was stronger in isolates cultured on PDB compared to YMB. From isolate TP 3 cultured in PDB, several secondary metabolite fractions were isolated, with fraction 4 (F4) containing linoleic acid as the pure compound. F4 demonstrated a Minimum Inhibitory Concentration (MIC) of 200 µg/mL against S. aureus, comparable to the TP 3 PDB isolate, while against E. coli, F4 showed MIC > 200 µg/mL and TP 3 PDB isolate showed an MIC of 100 µg/mL.
Evaluasi Aktivitas Antioksidan dan Antibakteri Dari Daun Empat Tumbuhan Suku Baduy Sebagai Sumber Bahan Alam Potensial Permatasari, Vera; Riskiana, Angger Arfi; Saefurohman, Asep; Prastya, Muhammad Eka; Ridha, Rafifa; Primahana, Gian; Dewijanti, Indah Dwiatmi
Jurnal Sumberdaya Hayati Vol. 11 No. 3 (2025): 2025
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.11.3.122-129

Abstract

Baduy indigenous plants have great potential as natural sources of bioactive compounds, yet scientific studies on their biological activities remain limited. This study aimed to evaluate the antioxidant, antibacterial activities, and total phenolic content of the leaves from four Baduy plant species: Gardenia jasminoides, Strobilanthes crispus, Ficus callosa, and Artocarpus camansi. The leaves were extracted with methanol, followed by fractionation using n-hexane and ethyl acetate. Antioxidant activity was assessed using DPPH and ABTS assays, total phenolic content was determined by the Folin–Ciocalteu method, and antibacterial activity was tested against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, and Escherichia coli using the broth dilution method to determine Minimum Inhibitory Concentration (MIC). The results showed that the ethyl acetate fraction of A. camansi leaves exhibited the highest total phenolic content (23.53 mg GAE/g extract). This fraction demonstrated strong antioxidant activity in the ABTS assay (IC50 = 18.55 ppm) and moderate activity in the DPPH assay (IC50 = 50.67 ppm). Moreover, it showed strong antibacterial activity against S. aureus, B. subtilis, and P. aeruginosa. These findings indicate that A. camansi leaves possess promising potential as a natural source of antioxidant and antibacterial agents.
Aktivitas Antibakteri dan Antioksidan dari Senyawa Bioaktif Asal Bakteri Endofit Tanaman Nyatoh (Palaquium amboinense B.) : Antibacterial and Antioxidant Properties of Bioactive Compounds Derived from Endophytic Bacteria Isolated from Nyatoh (Palaquium amboinense B.) Putra, Rayhan Helmyana; Retnowati, Dwi; Cordova, Datu Muhammad; Zahra, Aliya Azkia; Primahana, Gian; Dewi, Rizna Triana; Filailla, Euis; Sukirno; Prastya, Muhammad Eka
Jurnal Sumberdaya Hayati Vol. 10 No. 1 (2024): 2024
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.10.1.25-32

Abstract

Nyatoh plant (Palaquium amboinense B.) is native to Indonesia which is known to have the ability to produce potential secondary metabolites. The aim of this study was to evaluate the antibacterial and antioxidant activities from bioactive compounds produced by endophytic bacteria from nyatoh leaves. Five endophytic bacteria were tested against 4 targeted bacteria including Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 15442, Bacillus subtilis ATCC 19659, and Staphylococcus aureus ATCC 6538. Bacterial isolate which had the strongest antibacterial activity was then fermented and extracted. The corresponding extract was further tested on disc diffusion method, determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values, antioxidant evaluation, and analyzed for its bioactive compound components via GC-MS analysis. The results showed that one isolate, namely D1 can produce secondary metabolite which act as antibacterial and antioxidant potentials. This corresponding extract has the strongest MIC and MBC against B. subtilis with value of 39.5 and >78.1 µg/ml and exhibited antioxidant activity against 2,2-difenil-1-pikrilhidrazil (DPPH) with inhibition value of 85.37%. Based on GC-MS analysis, secondary metabolites derived from this potential extract contained some major active constituents including Tributyl acetylcitrate and bis (2-ethylhexyl) ester which might act as antibacterial and antioxidant agents.
ISOLATION AND IDENTIFICATION OF APIGENIN, A FLAVONOID COMPOUND FROM MACARANGA HYPOLEUCA (REICHB.F. & ZOLL.) Aisya, Sitti; Megawati, Megawati; Ariani, Novita; Sukirno, Sukirno; Minarti, Minarti; Kurniawan, Hendris Hendarsyah; Hidayat, Arief; Hendra, Medi; Primahana, Gian; Darmawan, Akhmad
Berita Biologi Vol 23 No 1 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2024.3242

Abstract

The study on Macarang hypolueca (Reichb.f. & Zoll.), which was collected from secondary forests around Samarinda City, East Kalimantan, involved phytochemical investigations that led to the isolation of a flavone type compound of flavonoid from the ethyl acetate fraction. To separate the compounds, silica gel column chromatography was utilized with a gradient solvent system of n-hexane and ethyl acetate, along with the addition of 5%. Infrared analysis (FTIR), mass spectrum (LC-ESI-MS), and nuclear magnetic resonance (1D- and 2D-NMR) were used to identify and elucidate the structure. Based on spectroscopic data and comparison with appropriate references, the isolated compound was identified as apigenin
Co-Authors A.A. Ketut Agung Cahyawan W Abda Abda Ace Tatang Hidayat Ahmad Randy Aisya, Sitti Akhmad Darmawan Amalia, Senya Putri Anggraini, Meisi Anisa, Hida Arliani Nur Anjayani, Nuri Ariani, Novita Asep Saefurohman Asih Triastuti Atwita, Syelziva Yonda Barokah, Giri Rohmad Chang, Helen Cordova, Datu Muhammad Desi Harneti Putri Huspa Dewijanti, Indah D. Dewijanti, Indah Dwiatmi Dwi Retnowati Eti Nurwening Sholikhah Febi Nurilmala Fikriyah, Annisaa Nurul Filailla, Euis Harlita Harlita Hasidu, La Ode Abdul Fajar Hendris Hendarsyah Kurniawan, Hendris Hendarsyah Hening, Egiyanti Nur Widhia Hidayatullah, Sigit Hutagaol, Ricson Pemimpin Iffa Afiqa Khairani Imaniar, Lisana Husna Jekmal Malau Jepri Agung Priyanto, Jepri Agung Khalijah Awang Linar Zalinar Udin, Linar Zalinar LINDA SUKMARINI Mario Mario Maulidiyah Medi Hendra, Medi Megawati Megawati - Meti Indrowati Minarti Minarti Muhammad Nurdin N. Nurjanah Permatasari, Vera Prastya, Muhamad Eka Prastya, Muhammad Eka Prima, Sylvia Rizky Pulung, Maria Ludya Putra, Rayhan Helmyana Respati Tri Swasono Rhesi Kristiana Riana, Elisa Nurma Ridha, Rafifa Riskiana, Angger Arfi Rizna Triana Dewi Rosita, Lisa Rosmalena, Rosmalena Satyaningsih, Desy Shiono, Yoshihito Simbolon, Sumihartati Slamet Santosa Sofa Fajriah Sri Pujiyanto SUKIRNO Sukirno Sukirno Suryanti, Erma Tamhid, Hady Anshory TATI NURHAYATI Tjandrawati Mozef Tri Joko Raharjo UMI FATMAWATI Unang Supratman Vivitri Dewi Prasasty yati, indri Yogaswara, Radite Yuswan, Apriza Zahra, Aliya Azkia Zienitha, Anggie Meilinda