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The potential of local orange peel-derived eco-enzymes in producing indole acetic acid Siska Alicia Farma; Nurfa Dewiza Luzik; Salma Sakina; Irma Leilani Eka Putri; Linda Advinda; Azwir Anhar
Acta Biochimica Indonesiana Vol. 6 No. 2 (2023): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.135

Abstract

Background: Ecoenzymes, created from the fermentation of organic citrus waste, offer a sustainable method to produce Indole Acetic Acid (IAA), a phytohormone vital for plant growth. This study investigates the potential of these ecoenzymes in promoting sustainable agriculture. Objective: This study aims to evaluate the capacity of ecoenzymes derived from local citrus organic waste to synthesize IAA hormones. Methods: The ecoenzyme was extracted from fruit powders and centrifuged to separate the supernatant. One ml of ecoenzyme supernatant was then mixed with 2 ml of Salkowski reagent and incubated for 12 hours at room temperature in the dark to facilitate reaction. The presence and concentration of IAA were determined using spectrophotometry at a wavelength of 530 nm, while total protein levels were measured using the Warburg-Christian method. Results: ecoenzymes from local citrus sources contain IAA, with the highest concentration observed in sample 7A (30.26 µg/ml). The ecoenzyme exhibited favorable characteristics, including an average degree of acidity of 3.55, and the highest total protein content was found in sample 2A (144.277 mg/mL). Conclusion: Ecoenzymes from local orange peels successfully produce IAA, supported by fermentation-induced microbial activity and acidic conditions. This highlights their potential in sustainable agriculture.
The Role of Plant Growth Promoting Rhizobacteria (PGPR) in Sustainable Agriculture Huda Asra, Rahmatul; Advinda, Linda; Anhar, Azwir; Irdawati
Jurnal Serambi Biologi Vol. 9 No. 1 (2024): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/srmb.v9i1.306

Abstract

The majority of farmers currently use chemical fertilizers and pesticides in farming. However, continuous use of these materials can cause negative impacts such as environmental pollution, health risks, nutrient cycle imbalances, and reduced crop yields. To overcome this problem, research has been conducted that focuses on the use of microorganisms found in the soil layer surrounding plant roots, called the rhizosphere. These microorganisms such as bacteria, fungi and protozoa form a group known as Plant Growth Promoting Rhizobacteria (PGPR). PGPR can increase plant growth through various mechanisms such as nitrogen fixation, phosphorus dissolution, production of cyanide acid compounds (HCN), and Indole Acetic Acid (IAA) compounds. This research aims to collect and analyze articles related to the role of PGPR in sustainable agriculture. The method used is a literature review by collecting sources from Google Scholar and other internet databases. The results of the articles collected were on topics related to PGPR, including mechanisms of nitrogen fixation and phosphorus solubilization, as well as their role in producing IAA and siderophores. Apart from that, the research also discusses the role of PGPR as a biocontrol agent that can control plant pathogens, and the antagonistic activity of PGPR against pathogens.
Potential of Pseudomonads Fluorescents as Plant Disease Biocontrol Agents Rusendra, Nabilla Makra; Advinda, Linda; Chatri, Moralita; Handayani, Dezi
Jurnal Serambi Biologi Vol. 9 No. 1 (2024): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/srmb.v9i1.320

Abstract

Fluorescent pseudomonas are a group of rhizobacteria that colonize plant root areas and have potential as biocontrol agents for plant diseases that can be developed. This group of bacteria can increase plant growth by producing growth hormones, one of which is producing hormones Indole Acetic Acid (IAA), solubilizing phosphate, and siderophores. Apart from that, fluorescent pseudomonas are also able to induce plant resistance by producing secondary metabolite compounds which have antimicrobial properties. Many studies have shown that one of the most abundant microorganisms in the rhizosphere (around the roots) is bacteria Pseudomonas spp. This review will discuss the occurrence, distribution, growth studies, mechanisms of action, and control of plant diseases by fluorescent pseudomonad bacteria. This literature review will be helpful for future research aimed at providing information that fluorescent pseudomonads have potential as biocontrol agents for plant diseases.
Pseudomonas Fluorescent As A Biocontrol Agent Against Plant Pathogens Dilla, Aprina; Advinda, Linda; Handayani, Dezi; Chatri, Moralita
Jurnal Serambi Biologi Vol. 9 No. 1 (2024): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/srmb.v9i1.321

Abstract

The use of biological control agents as alternatives to synthetic fungicides is increasingly being developed as farmers become more aware of the negative effects of synthetic fungicides. Various biocontrol agents have been developed from several bacteria that are very effective against plant diseases, including pseudomonas fluorescent. The mechanism of bacterial inhibition against pathogens can take place through several ways, namely colonization, competition and antibiosis. Pseudomonas fluorescent is able to produce secondary metabolite compounds such as siderophores, HCN, antibiotics, enzymes that can inhibit the growth and development of pathogens and can induce systemic resistance.
The Role of Phosphate Solubilizing Bacteria in Sustainable Agriculture Arlina, Sistika; Advinda, Linda; Chatri, Moralita; Putri, Dwi Hilda
Jurnal Serambi Biologi Vol. 9 No. 1 (2024): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/srmb.v9i1.338

Abstract

The repeated and unwise use of chemical fertilizers agricultural land can cause various negative impacts such as disrupting natural microbes and losing soil fertility. Phosphate solubilizing bacteria are soil bacteria that can convert phosphate from insoluble to soluble so that it can be absorbed by plants. Phosphate solubilizing bacteria play an important role in increasing plant productivity. Although the availability of phosphorus (P) in soil is in high concentrations, only 0.1% of the total phosphorus is available to plants and represents a small portion of the total in the soil. This occurs because of the low level of solubility of phosphorus and its fixation ability in the soil with several other metal elements in the soil such as Al, Ca, Fe to form aluminum phosphate, calcium phosphate and iron phosphate. There is no availability of phosphate for plants so that the role of phosphate-soluble bacteria is needed which plays a role in providing phosphate for plants so that it can increase agricultural yields. Phosphate solubilizing bacteria have great potential as biofertilizers because they can increase the bioavailability of phosphorus for plants, promote sustainable agriculture, and increase soil fertility, and increase crop yields.
Total Bakteri Asam Laktat (BAL) dari Sauerkraut Kubis Singgalang (Brassica oleracea var. capitata L.) dengan Konsentrasi Garam yang Berbeda: Total Lactic Acid Bacteria (LAB) from Sauerkraut of Singgalang Cabbage (Brassica oleracea var. capitata L.) with Addition of Various Salt Concentration Niken Maharani Putri; Resti Fevria; Linda Advinda; Moralita Chatri
Jurnal Sains dan Kesehatan Vol. 5 No. SE-1 (2023): Spesial Edition J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v5iSE-1.2062

Abstract

Lactic Acid Bacteria are a group of bacteria that produce lactic acid as their main product in the fermentation process and are probiotic agents that are good and safe for humans. LAB is found in many fermented processed food products, one of which is sauerkraut. Sauerkraut is a fermented product that only uses cabbage and salt in the manufacturing process. Sauerkraut can be made from various types of cabbage compatriots such as for example Singgalang Cabbage (Brassica oleracea var. capitata L.). Cabbage can be fermented into sauerkraut using a certain concentration of salt. The research was carried out with 3 treatment groups, namely Singgalang Cabbage Sauerkraut with salt concentrations of 2%, 2.5%, and 3%. The results showed that the highest total LAB was found in the addition of 2.5% salt, namely 186.67×106 cfu/ml. Meanwhile, the total LAB at concentration of 2% was 83.33×106 cfu/ml and at concentration of 3%, it was 51×106 cfu/ml. This amount has met the minimum standards of LAB in each product and meets the minimum standards to be beneficial for human body. Keywords: Lactic Acid Bacteria, Sauerkraut Abstrak Bakteri Asam Laktat merupakan kelompok bakteri penghasil asam laktat sebagai produk utamanya dalam proses fermentasi dan merupakan agen probiotik yang baik dan aman bagi manusia. BAL banyak ditemukan pada produk makanan olahan fermentasi salah satunya pada sauerkraut. Sauerkraut  merupakan produk fermentasi yang hanya menggunakan kol dan garam dalam proses pembuatannya. Sauerkraut dapat dibuat dari berbagai jenis sayuran sebangsa kol seperti contohnya Kubis Singgalang (Brassica oleracea var. capitata L.). Kol dapat difermentasi menjadi sauerkraut menggunakan garam dengan konsentrasi tertentu. Penelitan dilakukan dengan 3 kelompok perlakuan yaitu Sauerkraut Kubis Singgalang dengan pemberian konsentrasi garam 2%, 2,5%, dan 3%. Hasil penelitian didapatkan bahwa total BAL terbanyak terdapat pada penambahan garam 2,5% yaitu 186,67×106 cfu/ml. Sedangkan total BAL pada konsentrasi 2% yaitu 83,33×106 cfu/ml dan konsentrasi 3% yaitu 51×106 cfu/ml. Jumlah tersebut sudah memenuhi standar minimum BAL dalam tiap produk dan memenuhi standar minimum agar bermanfaat bagi tubuh manusia. Kata Kunci: Bakteri Asam Laktat, Sauerkraut
Training on The Utilization of Coconut Water Into Nata De Coco A Food Product With High Fiber Content in Communities in Nagari Tanjung Balik, Solok District Advinda, Linda; Irdawati, Irdawati; Syahrastani, Syahrastani; Rahmi, Elva; Larashinda, Mentari
Pelita Eksakta Vol 7 No 2 (2024): Pelita Eksakta, Vol. 7, No. 2
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol7-iss2/246

Abstract

The use of coconut water by the community is still rarely done, even though coconut water can be used as a basic ingredient for making nata de coco. Nata de coco is a processed product that is quite popular in Indonesia. The aim of the service activity is to provide training in making nata de coco to PKK women in Nagari Tanjung Balik, Solok District. Training on making nata de coco was carried out considering that PKK women in Nagari Tanjung Balik really need knowledge and skills that can be used as provisions to start a business. The training provided starts with an introduction to nata de coco seeds, the manufacturing process, and packaging nata de coco. After receiving training, participants who initially did not know about nata de coco became aware of it and were able to produce nata de coco.
Isolasi Staphylococcus Aureus Pada Jajanan di SMA 1 Pertiwi Padang Sari, Feby Djumaita; Advinda, Linda
Jurnal Pendidikan Tambusai Vol. 9 No. 1 (2025)
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai, Riau, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jptam.v9i1.25290

Abstract

Keamanan pangan di lingkungan sekolah merupakan aspek fundamental dalam menjaga kesehatan dan para siswa, khususnya mereka yang terbiasa mengonsumsi jajanan. Jajanan yang diolah dengan cara yang tidak tepat dapat menjadi media kontaminasi bakteri berbahaya, seperti Staphylococcus aureus yang berpotensi menimbulkan berbagai penyakit dan gangguan kesehatan. Penelitian ini bertujuan untuk mengidentifikasi dan mengetahui tingkat kontaminasi Staphylococcus aureus pada jajanan di SMA 1 Pertiwi Padang. Penelitian ini dilakukan dengan cara mengisolasi sampel jajanan yang didapatkan dari 3 penjual di sekitar SMA 1 Pertiwi Padang. Hasil penelitian menunjukan tingkat kontaminasi Staphylococcus aureus yang tinggi pada ketiga sampel jajanan dengan karakteristik makroskopis warna koloni kuning keemasan, bentuk dan ukuran bulat kecil & titik, dan elevasi koloni convex. Dan karakteristik mikroskopis bentuk bakteri yang bulah (coccus) serta susunan bakteri yang berantai seperti anggur (stapilokokus). Kombinasi paparan udara, suhu, dan kelembaban yang tinggi merupakan faktor yang meningkatkan risiko kontaminasi Staphylococcus aureus pada jajanan.
Deteksi Staphylococcus Aureus Pada Beberapa Jenis Jajanan Di SD Negeri 19 Air Tawar Padang Aprilika, Kenny; Linda Advinda
Jurnal Biologi dan Pembelajarannya (JB&P) Vol 12 No 1 (2025): APRIL 2025
Publisher : Universitas Nusantara PGRI Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29407/jbp.v12i1.25007

Abstract

Keamanan jajanan sekolah menjadi perhatian utama bagi orang tua, pendidik, dan pengelola sekolah dasar karena risiko kontaminasi oleh bahan biologis, fisik, dan kimia yang dapat membahayakan kesehatan anak. Penelitian ini bertujuan untuk mendeteksi keberadaan bakteri Staphylococcus aureus pada jajanan di SD Negeri 19 Air Tawar Barat Padang. Isolasi Staphylococcus aureus dilakukan terhadap 3 jenis jajanan dan medium yang digunakan Mannitol Salt Agar (MSA). Pengamatan penelitian adalah jumlah bakteri Staphylococcus aureus, pengamatan makroskopis dan mikroskopisnya. Hasil penelitian menunjukkan bahwa semua sampel jajanan dari SD Negeri 19 Air Tawar Barat Padang mengandung bakteri Staphylococcus aureus. Morfologi makroskopisnya beragam: pada sampel K1 (tahu isi), terdapat 49.105 CFU dengan ciri-ciri serupa pada sampel K2 (shanghai), sedangkan pada sampel K3 (sosis goreng), terdapat 25.105 CFU dengan karakteristik yang berbeda. Secara mikroskopis, bentuk dan susunan bakteri S.aureus  tetap konsisten sebagai coccus dengan susunan stafilokokus.
LITERATUR REVIEW: SENYAWA AKTIF TUMBUHAN YANG EFEKTIF SEBAGAI PESTISIDA NABATI UNTUK PENGENDALIAN PENYAKIT TANAMAN Nadia; Chatri, Moralita; Advinda, Linda; Putri, Dwi Hilda
JURNAL BIOSENSE Vol 8 No 1 (2025): Edisi Januari 2025
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v8i1.5028

Abstract

Abstrak Perlunya pengendalian penyakit tanaman yang ramah lingkungan mendorong dikembangkannya fitopestisida berbahan aktif senyawa tanaman. Senyawa aktif seperti alkaloid, flavonoid, saponin, tanin dan minyak atsiri terbukti efektif mengendalikan patogen tanaman sekaligus mengurangi efek negatif pestisida kimia. Senyawa tersebut berasal dari bagian tumbuhan seperti akar, batang, daun, bunga dan buah. Penelitian ini bertujuan untuk mengumpulkan dan menganalisis artikel terkait efektivitas senyawa aktif pada tanaman sebagai pestisida nabati. Perancangan menggunakan literatur review yang dikumpulkan melalui mesin pencari seperti PubMed, Google Scholar, dan ScienceDirect. Kriteria artikel yang digunakan adalah artikel terbitan tahun 2019 hingga 2024 yang membahas tentang penggunaan pestisida tanaman untuk mengendalikan penyakit tanaman. Hasil analisis menunjukkan bahwa senyawa aktif pada tanaman dapat menghambat pertumbuhan patogen dengan berbagai cara, misalnya dengan merusak membran sel patogen, mengganggu metabolisme, dan menghentikan beberapa enzim. Oleh karena itu, pestisida nabati dapat digunakan untuk pertanian organik dan kelestarian lingkungan.
Co-Authors A. Nabilla Nabilla Achyar, Afifatul Ade Indra Utama Aditya Willy Putra Ahmad Wibisana, Ahmad Aissa Febrya, Malika Alya Fariani Fariani Amelya Ningsih, Putri Andesmora, Evan Vria Andriani Andriani Aprilika, Kenny Arlina, Sistika Atifah, Yusni Atiqahh Muthiah Aulia, Fadila Azwir Anhar Beni Gusnadi Betti Anggrayeni Chahya, Anisha Claudia Ayesha Dartiani Sagurung, Mariance Delfi Suganda Des M Devi Aprillia Sary Dezi Handayani Dhea Sabrina Aprilia Dilla, Aprina Dina Sukma Dwi Hilda Putri Eka Nuraini, Fauzi Elsa Badriyya Elsa Yuniarti Ernawati Ernawati Febri Doni Febrianti, Liza Feby Yeriska Fevria, Resti Fitri Angraini Fitri Arsih Fitri, Ryantika Fitria, Davina Fronica, Imelda Ghiffari, Muhammad Hamdah, Alya Handayani, Delsi Fitri Haqil Triyatdipa Hervi, Fauziah Huda Asra, Rahmatul Husnul Khatimah Idos Susila Ningsih Ilham Pratama Inayatul Fatia Indra Hartanto Indrawani Matondang Intan Febriani Irawati, Wike IRDAWATI Irdawati Irdawati Irma Leilani Irma Leilani Eka Putri Irma Leilani Eka Putri Jannah Koftiah Jumatul Hafsah Kiki Amelia Kiki Amelia, Kiki Larashinda, Mentari Lati Jovanita Listi Handayani M Maizirwan Mades Fifendy Mantoviana, Tiffany Mardhiyah Nazri, Laila Marisa Marisa Marsha Utami Maysyarah Ardiana Mentari Larashinda Milka Saputri Moralita Chatri Muhammad Deedat Ayasy Mukhlis Mukhlis Mulia Mulia Mulia Mulia, Nanta Mulyadi, Rival Nabilah, Rezi Nadia Nazri, Laila Mardhiyah Nella Fauziah Niken Maharani Putri Nola Nurdianata Norman, Mayanda Novi Novi Nur Vaizi Nuraini, Fauzi Eka Nurfa Dewiza Luzik Nurfatihah Z, Zahara Pratama Putra, Abil Puspa Anggraeni Putri Putri Andam Dewi Putri, Santi Diana Rahmadhani Fitri Rahmawita Rahmawita Rahmawita Rahmawita Rahmi Holinesti Rahmi, Elva Ramadhan Sumarmin Resti Yulia Rusendra, Nabilla Makra S. Syamsurizal Salma Sakina Salsabilla, Vishtari Sari, Feby Djumaita sari, Mutia Selly Fildawati Silviana Okwisan Simbolon, Perima Siska Alicia Farma Situmorang, Rivaldo Sri Rahmadani Fitri Syahrastani, Syahrastani Syamsurizal Syamsurizal - - Syamsurizal Syamsurizal Taufiqqurrahman Taufiqqurrahman Vauzia, Vauzia Violita Violita Violita Violita Viona, Alda Wilna Sari Wilna Sari Winardi, Fhuji Windi Nopitasari Wulandari, Tesya Yossi Rahmadeni Yulanda Saputri Yuni Ahda Yunis Marni Yusrizal Y Yusrizal Yusrizal Yusrizal Yusrizal Zen, Muhammad Nazif Zulyusri, Zulyusri